AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0256790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256790 MtrunA17_Chr2g0318561 99.394 495 3 0 135 629 2 496 0.0 1019
Msa0256790 MtrunA17_Chr8g0360821 68.903 611 129 6 76 627 63 671 0.0 866
Msa0256790 MtrunA17_Chr7g0218341 50.778 514 236 6 113 622 206 706 4.82e-177 519
Msa0256790 MtrunA17_Chr2g0326871 37.876 499 288 10 120 612 177 659 1.13e-105 335
Msa0256790 MtrunA17_Chr4g0028861 39.785 465 263 9 120 579 224 676 2.36e-105 333
Msa0256790 MtrunA17_Chr3g0139071 39.121 478 269 11 123 591 181 645 6.84e-100 320
Msa0256790 MtrunA17_Chr3g0125651 37.764 474 269 9 123 583 139 599 2.32e-98 315
Msa0256790 MtrunA17_Chr3g0126541 33.552 611 315 18 44 580 41 634 2.79e-98 316
Msa0256790 MtrunA17_Chr2g0305771 37.980 495 282 11 123 608 151 629 8.93e-98 314
Msa0256790 MtrunA17_Chr1g0158861 31.803 610 321 14 66 610 74 653 3.38e-96 310
Msa0256790 MtrunA17_Chr1g0181121 37.393 468 274 8 120 581 144 598 3.46e-96 309
Msa0256790 MtrunA17_Chr4g0073941 37.580 471 264 12 123 580 203 656 1.01e-94 307
Msa0256790 MtrunA17_Chr4g0001481 35.319 470 285 9 120 580 202 661 1.51e-89 293
Msa0256790 MtrunA17_Chr7g0228181 36.688 477 284 11 123 590 147 614 2.12e-89 291
Msa0256790 MtrunA17_Chr7g0275581 35.593 472 272 8 123 580 187 640 6.35e-89 291
Msa0256790 MtrunA17_Chr8g0347121 32.800 500 287 10 123 583 164 653 3.19e-81 271
Msa0256790 MtrunA17_Chr6g0481021 34.864 479 289 8 123 588 227 695 4.84e-79 264
Msa0256790 MtrunA17_Chr6g0480851 33.659 511 307 10 90 585 255 748 8.01e-78 262
Msa0256790 MtrunA17_Chr6g0480781 29.630 513 325 11 90 585 240 733 1.47e-75 256
Msa0256790 MtrunA17_Chr6g0480671 35.759 481 281 12 123 590 297 762 2.09e-70 243
Msa0256790 MtrunA17_Chr6g0480741 29.020 510 324 13 90 582 186 674 2.90e-59 210
Msa0256790 MtrunA17_Chr6g0480681 29.955 444 273 12 156 584 280 700 4.29e-50 186
Msa0256790 MtrunA17_Chr2g0318551 61.878 181 12 4 14 139 1 179 5.78e-47 164
Msa0256790 MtrunA17_Chr5g0395421 32.961 358 207 8 217 561 18 355 2.64e-40 151
Msa0256790 MtrunA17_Chr6g0480771 33.333 249 146 6 345 581 12 252 3.53e-32 126
Msa0256790 MtrunA17_Chr6g0480841 36.181 199 110 5 399 585 2 195 5.53e-26 106
Msa0256790 MtrunA17_Chr2g0326861 30.357 168 105 3 120 286 40 196 4.53e-21 92.4
Msa0256790 MtrunA17_Chr5g0395411 29.771 131 88 2 90 218 230 358 3.13e-15 77.8
Msa0256790 MtrunA17_Chr2g0326851 38.462 91 52 2 522 612 3 89 4.26e-12 63.9
Msa0256790 MtrunA17_Chr6g0480821 20.571 175 125 3 136 308 25 187 5.22e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256790 AT5G54250.2 75.810 525 123 3 104 627 171 692 0.0 823
Msa0256790 AT5G54250.1 75.810 525 123 3 104 627 171 692 0.0 823
Msa0256790 AT5G54250.4 75.810 525 123 3 104 627 171 692 0.0 823
Msa0256790 AT5G54250.3 74.857 525 123 4 104 627 171 687 0.0 806
Msa0256790 AT5G15410.2 47.573 515 262 6 113 622 210 721 3.39e-158 472
Msa0256790 AT5G15410.1 47.573 515 262 6 113 622 211 722 5.44e-158 471
Msa0256790 AT2G28260.2 39.787 470 264 8 120 583 128 584 1.19e-108 341
Msa0256790 AT2G28260.1 39.787 470 264 8 120 583 168 624 2.23e-108 342
Msa0256790 AT5G57940.1 39.662 474 259 11 123 583 192 651 1.04e-100 322
Msa0256790 AT5G57940.2 39.662 474 259 11 123 583 192 651 1.04e-100 322
Msa0256790 AT5G57940.3 39.662 474 259 11 123 583 185 644 1.30e-100 322
Msa0256790 AT2G24610.1 38.365 477 274 11 123 591 178 642 6.09e-99 318
Msa0256790 AT2G24610.2 38.365 477 274 11 123 591 193 657 2.55e-98 317
Msa0256790 AT2G23980.6 38.125 480 271 10 123 589 180 646 1.21e-96 312
Msa0256790 AT5G53130.1 38.116 467 262 10 123 580 188 636 1.47e-96 311
Msa0256790 AT2G23980.9 38.125 480 271 10 123 589 207 673 2.41e-96 312
Msa0256790 AT2G23980.10 38.125 480 271 10 123 589 207 673 2.41e-96 312
Msa0256790 AT2G23980.1 38.125 480 271 10 123 589 207 673 2.41e-96 312
Msa0256790 AT2G23980.4 38.125 480 271 10 123 589 207 673 2.41e-96 312
Msa0256790 AT2G23980.5 38.125 480 271 10 123 589 207 673 2.41e-96 312
Msa0256790 AT1G01340.2 36.235 494 284 9 123 610 172 640 3.86e-96 310
Msa0256790 AT4G30360.1 37.238 478 279 9 123 592 179 643 4.60e-96 310
Msa0256790 AT1G01340.1 36.235 494 284 9 123 610 167 635 5.07e-96 310
Msa0256790 AT1G15990.1 37.657 478 264 11 123 583 195 655 1.02e-95 310
Msa0256790 AT1G19780.1 37.447 470 276 10 123 583 201 661 3.17e-95 309
Msa0256790 AT4G01010.1 35.758 495 286 10 123 610 172 641 5.99e-95 307
Msa0256790 AT4G01010.2 35.758 495 286 10 123 610 172 641 5.99e-95 307
Msa0256790 AT4G30560.5 37.866 478 274 11 123 589 181 646 6.90e-95 307
Msa0256790 AT4G30560.2 37.866 478 274 11 123 589 181 646 6.90e-95 307
Msa0256790 AT4G30560.3 37.866 478 274 11 123 589 181 646 6.90e-95 307
Msa0256790 AT4G30560.1 37.866 478 274 11 123 589 207 672 1.57e-94 306
Msa0256790 AT4G30560.4 37.866 478 274 11 123 589 207 672 1.57e-94 306
Msa0256790 AT5G14870.1 37.238 478 281 11 123 591 143 610 4.20e-94 305
Msa0256790 AT1G19780.2 38.391 435 253 8 157 583 23 450 3.38e-93 298
Msa0256790 AT3G48010.1 36.632 475 275 9 123 583 148 610 1.16e-91 298
Msa0256790 AT2G23980.3 36.382 503 271 11 123 589 207 696 4.58e-91 298
Msa0256790 AT2G23980.2 36.382 503 271 11 123 589 207 696 4.58e-91 298
Msa0256790 AT2G23980.8 36.382 503 271 11 123 589 207 696 4.58e-91 298
Msa0256790 AT2G23980.7 36.382 503 271 11 123 589 207 696 4.58e-91 298
Msa0256790 AT2G46430.1 35.020 494 286 12 123 609 176 641 5.18e-86 283
Msa0256790 AT2G46430.2 35.020 494 286 12 123 609 176 641 5.18e-86 283
Msa0256790 AT3G17690.1 33.663 505 306 13 90 583 229 715 5.67e-80 268
Msa0256790 AT3G17700.1 36.481 466 269 10 123 577 296 745 1.98e-78 265
Msa0256790 AT2G46450.2 32.845 478 276 15 123 583 110 559 6.55e-70 238
Msa0256790 AT2G46450.3 32.845 478 276 15 123 583 97 546 1.14e-69 238
Msa0256790 AT2G46450.4 32.845 478 276 15 123 583 133 582 1.25e-69 238
Msa0256790 AT2G46450.1 32.845 478 276 15 123 583 133 582 1.25e-69 238
Msa0256790 AT3G17690.2 33.254 421 261 10 90 507 229 632 4.97e-67 231
Msa0256790 AT3G17700.2 37.113 388 227 8 123 509 296 667 9.03e-67 231
Msa0256790 AT2G46440.1 32.008 503 290 15 123 609 133 599 3.21e-60 212
Msa0256790 AT2G46440.2 32.569 436 255 10 187 609 12 421 1.47e-56 198
Msa0256790 AT3G17690.3 30.333 300 194 7 90 386 229 516 1.86e-37 146
Msa0256790 AT5G37500.4 24.651 215 155 5 304 515 261 471 3.89e-14 76.3
Msa0256790 AT5G37500.2 24.766 214 154 5 305 515 262 471 5.02e-14 76.3
Msa0256790 AT5G37500.1 24.766 214 154 5 305 515 262 471 5.16e-14 75.9
Msa0256790 AT5G37500.3 24.766 214 154 5 305 515 208 417 5.99e-14 75.9
Msa0256790 AT3G02850.3 21.860 215 161 5 305 516 279 489 4.71e-12 69.3
Msa0256790 AT3G02850.1 21.963 214 160 5 305 515 279 488 5.95e-12 69.3
Msa0256790 AT3G02850.2 21.963 214 160 5 305 515 279 488 6.18e-12 69.3

Find 129 sgRNAs with CRISPR-Local

Find 323 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTACAACAGTCGAAGTATT+TGG 0.199388 2_2:+51849238 Msa0256790:CDS
GGTGATGTTGTCAGCACTTT+TGG 0.210717 2_2:+51848104 Msa0256790:intron
AGATACTCCTTCCTATCTTT+TGG 0.215218 2_2:+51847336 Msa0256790:CDS
CTTCTAGTCACTATGCTAAT+TGG 0.241612 2_2:+51848194 Msa0256790:CDS
TTTGTCAAGGTCCACTTCTT+TGG 0.250073 2_2:+51849580 Msa0256790:CDS
AAACATAAACGCTCCTCTTT+TGG 0.273915 2_2:+51844791 Msa0256790:CDS
TATCAAGTACCATCTTTGTT+TGG 0.281982 2_2:+51848757 Msa0256790:CDS
AGTGCTGATAACTATGAATT+TGG 0.287072 2_2:+51847263 Msa0256790:CDS
TGAGTTCTTTGTCCTTTATT+CGG 0.292219 2_2:+51849546 Msa0256790:CDS
GATAAAGATGTTAAAGAAAA+AGG 0.300042 2_2:-51844867 None:intergenic
GTTGGAAGACGTTCTATAAA+TGG 0.307863 2_2:-51849193 None:intergenic
GGCTGCTGTGGCAATTCAAT+TGG 0.324383 2_2:+51849493 Msa0256790:CDS
ATCAAGTACCATCTTTGTTT+GGG 0.333486 2_2:+51848758 Msa0256790:CDS
TAACTCCATCTCTTAGGAAT+TGG 0.337128 2_2:-51849102 None:intergenic
ACTCACCTGGTTGGAGAACT+TGG 0.342981 2_2:+51849471 Msa0256790:CDS
AGGGTCATGTTGGTATTTGT+TGG 0.347194 2_2:+51847061 Msa0256790:CDS
TTATAGTTTCCATAGTATAT+TGG 0.355926 2_2:-51847167 None:intergenic
GTACCATCTTTGTTTGGGTT+TGG 0.357835 2_2:+51848763 Msa0256790:CDS
TCATCTCCACTGAAGTTTCC+TGG 0.360097 2_2:-51849145 None:intergenic
GAGTCAAAATCATCTTGATT+TGG 0.362918 2_2:-51849650 None:intergenic
AAATCATCTTGATTTGGCTT+TGG 0.367402 2_2:-51849644 None:intergenic
TGCAAAGAACCAATATACTA+TGG 0.378037 2_2:+51847158 Msa0256790:CDS
TAGACAGAACAAGGTTGGTT+TGG 0.386399 2_2:+51847204 Msa0256790:CDS
ATGAATTTGGAGTTTATGAA+TGG 0.394019 2_2:+51847276 Msa0256790:CDS
GGGTCATGTTGGTATTTGTT+GGG 0.397628 2_2:+51847062 Msa0256790:CDS
ACAAATAGCATTCTACGAAC+TGG 0.398087 2_2:-51849055 None:intergenic
AATTGGCTTGGAGAAGGTAT+AGG 0.400107 2_2:+51849510 Msa0256790:CDS
CAATTAGCATAGTGACTAGA+AGG 0.401970 2_2:-51848193 None:intergenic
TACTTGATCCAAGAGGAAAA+TGG 0.403260 2_2:+51844567 Msa0256790:CDS
TTAGGAATTGGCTACTTTGA+AGG 0.405487 2_2:-51849090 None:intergenic
TCTTCATAACATCGTGTTTG+TGG 0.412923 2_2:+51849671 Msa0256790:exon
GGCTTTGTTGGCTTTGAAGA+AGG 0.425788 2_2:-51844846 None:intergenic
AAACTTGAGTGATGTTGATC+GGG 0.429397 2_2:-51844350 None:intergenic
AGGCCTACGAGGCCGAATAA+AGG 0.432529 2_2:-51849558 None:intergenic
TCCAACATCATCATCGACTT+TGG 0.439571 2_2:+51849210 Msa0256790:CDS
TTGTTTGGGTTTGGTGAGAC+AGG 0.441635 2_2:+51848772 Msa0256790:CDS
CCATCCTTTCTGTTGTACTC+TGG 0.448749 2_2:-51848133 None:intergenic
ACTTGATCCAAGAGGAAAAT+GGG 0.451152 2_2:+51844568 Msa0256790:CDS
GTTAAAAGTTGTTGTATGCT+AGG 0.457056 2_2:+51849121 Msa0256790:CDS
GTCATTAGGCCCCAAAAGAT+AGG 0.457093 2_2:-51847347 None:intergenic
GTAGAATGCTATTTGTGGTA+AGG 0.461877 2_2:+51849062 Msa0256790:CDS
TTTGGGGCCTAATGACTCTC+AGG 0.466761 2_2:+51847354 Msa0256790:CDS
GAAATTAGACAGAACAAGGT+TGG 0.478036 2_2:+51847199 Msa0256790:CDS
CTGTGGCAATTCAATTGGCT+TGG 0.478572 2_2:+51849498 Msa0256790:CDS
GAGGAAAATGGGCTGAAGAA+TGG 0.486947 2_2:+51844579 Msa0256790:CDS
TTTCTCCCTTTGTGAATACA+AGG 0.489039 2_2:-51848928 None:intergenic
TATCCTCCTCCCTCTCCCTC+AGG 0.489371 2_2:+51844886 Msa0256790:CDS
CCTTCCTAAAATTTATCACT+CGG 0.489565 2_2:+51846767 Msa0256790:CDS
GTGCGTCGAAACCTCACAAA+TGG 0.493386 2_2:+51846798 Msa0256790:CDS
TCCTTTCTGTTGTACTCTGG+AGG 0.494095 2_2:-51848130 None:intergenic
TAGAATGCTATTTGTGGTAA+GGG 0.494337 2_2:+51849063 Msa0256790:CDS
AGTTCGTAGAATGCTATTTG+TGG 0.494344 2_2:+51849057 Msa0256790:CDS
AAAACTTGAGTGATGTTGAT+CGG 0.495921 2_2:-51844351 None:intergenic
AAGGTCCACTTCTTTGGAAG+AGG 0.502180 2_2:+51849586 Msa0256790:CDS
ATATCCCTTCTCAAACCCTC+CGG 0.502776 2_2:-51848737 None:intergenic
GATCCTTGCTCTAACAAAGA+TGG 0.503367 2_2:-51846696 None:intergenic
TCTATCCTCTTCCAAAGAAG+TGG 0.518250 2_2:-51849591 None:intergenic
GGTATTTGTTGGGACTTCAA+AGG 0.518699 2_2:+51847072 Msa0256790:CDS
TATATGGTGAAACAGGCAGC+AGG 0.520127 2_2:+51847041 Msa0256790:intron
TTACCTGAGGGAGAGGGAGG+AGG 0.520907 2_2:-51844889 None:intergenic
TTGGAGAACTTGGGCTGCTG+TGG 0.530447 2_2:+51849481 Msa0256790:CDS
TAACTACGAGAGACAGTGTT+GGG 0.530575 2_2:+51848670 Msa0256790:CDS
CAATTCAATTGGCTTGGAGA+AGG 0.530721 2_2:+51849504 Msa0256790:CDS
AAGTCCCTTGTATTCACAAA+GGG 0.534584 2_2:+51848923 Msa0256790:CDS
TGTCCTTTATTCGGCCTCGT+AGG 0.536746 2_2:+51849555 Msa0256790:CDS
CAAATAGCATTCTACGAACT+GGG 0.537490 2_2:-51849054 None:intergenic
ATTCCATCTTTGTTAGAGCA+AGG 0.537624 2_2:+51846693 Msa0256790:CDS
AGTAGCCAATTCCTAAGAGA+TGG 0.538975 2_2:+51849097 Msa0256790:CDS
TAAATAATTACCTGAGGGAG+AGG 0.541037 2_2:-51844896 None:intergenic
GAAAATACCTGAGAGTCATT+AGG 0.541685 2_2:-51847361 None:intergenic
GTATTTGTTGGGACTTCAAA+GGG 0.546964 2_2:+51847073 Msa0256790:CDS
CAGTGTTGGGCTGCCATGCG+CGG 0.554243 2_2:+51848683 Msa0256790:CDS
CTTGGAGAAGGTATAGGCAT+AGG 0.554858 2_2:+51849516 Msa0256790:CDS
ATGATGAATGAAGCCAAAAG+AGG 0.559467 2_2:-51844804 None:intergenic
TTAGATATTACTCACCTGGT+TGG 0.563077 2_2:+51849462 Msa0256790:CDS
CCAGAGTACAACAGAAAGGA+TGG 0.563680 2_2:+51848133 Msa0256790:CDS
GTGGAGATGAACTTCTATCA+TGG 0.565820 2_2:+51849158 Msa0256790:CDS
ATCTTGATTTGGCTTTGGTG+AGG 0.566095 2_2:-51849639 None:intergenic
TCACCAAACCCAAACAAAGA+TGG 0.566389 2_2:-51848766 None:intergenic
CTCACCTGGTTGGAGAACTT+GGG 0.567396 2_2:+51849472 Msa0256790:CDS
TGCATACAGTTAACAAGAGA+AGG 0.570005 2_2:+51849028 Msa0256790:intron
AACACTATTATATTAACTAG+TGG 0.571292 2_2:+51848167 Msa0256790:CDS
ACCAAAGTCGATGATGATGT+TGG 0.573525 2_2:-51849211 None:intergenic
GACGGTTGCCACAAGGACTT+CGG 0.574652 2_2:+51848636 Msa0256790:CDS
ACATTCATCAACGCCGCGCA+TGG 0.577989 2_2:-51848696 None:intergenic
TGTGAGACAACACCTGGTTG+TGG 0.579757 2_2:+51847119 Msa0256790:CDS
TATGCTAATTGGAAACATCA+AGG 0.582197 2_2:+51848205 Msa0256790:CDS
ATCTCCCGGAGGGTTTGAGA+AGG 0.585770 2_2:+51848732 Msa0256790:CDS
TATCCCTTCTCAAACCCTCC+GGG 0.586412 2_2:-51848736 None:intergenic
AGTGTTAGATATTACTCACC+TGG 0.588815 2_2:+51849458 Msa0256790:CDS
AACAGGCAGCAGGGTCATGT+TGG 0.591008 2_2:+51847051 Msa0256790:intron
TAATTACCTGAGGGAGAGGG+AGG 0.593631 2_2:-51844892 None:intergenic
AGTTGTTGTATGCTAGGACC+AGG 0.595053 2_2:+51849127 Msa0256790:CDS
ATCGTCGATGTCATCGTCAG+TGG 0.598201 2_2:-51844405 None:intergenic
GGCCTCGTAGGCCTTTGTCA+AGG 0.602074 2_2:+51849567 Msa0256790:CDS
TGAAGAAATTAGACAGAACA+AGG 0.603321 2_2:+51847195 Msa0256790:CDS
AAATAATTACCTGAGGGAGA+GGG 0.607454 2_2:-51844895 None:intergenic
GTGAGACAACACCTGGTTGT+GGG 0.608869 2_2:+51847120 Msa0256790:CDS
AACAAAAGACGGTTGCCACA+AGG 0.610620 2_2:+51848629 Msa0256790:CDS
TTAGGCCCCAAAAGATAGGA+AGG 0.610853 2_2:-51847343 None:intergenic
AACTTGAGTGATGTTGATCG+GGG 0.611958 2_2:-51844349 None:intergenic
GATAGCGAATCCCCACCAAA+CGG 0.616213 2_2:-51846835 None:intergenic
TCTCCCGGAGGGTTTGAGAA+GGG 0.616232 2_2:+51848733 Msa0256790:CDS
TTCTCCCTTTGTGAATACAA+GGG 0.616802 2_2:-51848927 None:intergenic
AGAGCAAGGATCAATTGCGC+GGG 0.622769 2_2:+51846707 Msa0256790:CDS
CAAGTCCCTTGTATTCACAA+AGG 0.623284 2_2:+51848922 Msa0256790:CDS
GCAGCCCAAGTTCTCCAACC+AGG 0.629666 2_2:-51849476 None:intergenic
CTAGGACCAGGAAACTTCAG+TGG 0.632219 2_2:+51849139 Msa0256790:CDS
ACCTCCAGAGTACAACAGAA+AGG 0.635309 2_2:+51848129 Msa0256790:CDS
AGAATGCTATTTGTGGTAAG+GGG 0.637257 2_2:+51849064 Msa0256790:CDS
TAGAGCAAGGATCAATTGCG+CGG 0.643328 2_2:+51846706 Msa0256790:CDS
GAGCAATGTGAGACAACACC+TGG 0.645036 2_2:+51847113 Msa0256790:CDS
AAAGAAGTGGACCTTGACAA+AGG 0.645468 2_2:-51849578 None:intergenic
ACACGCTGCCGAAGTCCTTG+TGG 0.648094 2_2:-51848644 None:intergenic
AGTGTTCTTAGCCCACAACC+AGG 0.653747 2_2:-51847131 None:intergenic
GAAATTGAAGATGACAAACA+TGG 0.653767 2_2:+51848595 Msa0256790:CDS
AGTATTTGGACTTGAAGCAG+AGG 0.666739 2_2:+51849252 Msa0256790:CDS
TGAAGATGACAAACATGGAG+TGG 0.674136 2_2:+51848600 Msa0256790:CDS
GGTCACAGAACACAGAAGCA+AGG 0.678907 2_2:+51847225 Msa0256790:CDS
ATATGGTGAAACAGGCAGCA+GGG 0.685470 2_2:+51847042 Msa0256790:intron
AGATGACAAACATGGAGTGG+TGG 0.685912 2_2:+51848603 Msa0256790:CDS
GTAACTACGAGAGACAGTGT+TGG 0.686561 2_2:+51848669 Msa0256790:CDS
GACCTTGACAAAGGCCTACG+AGG 0.688299 2_2:-51849569 None:intergenic
CGATAGCGACGAGTACGATG+AGG 0.693290 2_2:+51844451 Msa0256790:CDS
CGACGATATCGTCAACAACG+AGG 0.696721 2_2:+51844421 Msa0256790:CDS
TTAGCATAGTGACTAGAAGG+AGG 0.697558 2_2:-51848190 None:intergenic
AGGGTATTACTTGATCCAAG+AGG 0.706932 2_2:+51844560 Msa0256790:CDS
AGTGGTGGATGAACAAAAGA+CGG 0.713345 2_2:+51848618 Msa0256790:CDS
ACTTGAGTGATGTTGATCGG+GGG 0.715749 2_2:-51844348 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGTATTAAGATTAAATT+TGG + chr2_2:51845983-51846002 Msa0256790:intron 10.0%
!! ATAAAACAAATAAAATATGT+TGG - chr2_2:51846112-51846131 None:intergenic 10.0%
!!! ATAGAATATTTTAATTTGTT+TGG - chr2_2:51846573-51846592 None:intergenic 10.0%
!!! TGAAATTTTTAAAATGTATT+TGG + chr2_2:51846623-51846642 Msa0256790:intron 10.0%
!!! AACTTTTTATACTATATATA+TGG + chr2_2:51847025-51847044 Msa0256790:intron 10.0%
!!! AAATAAGTATTTTAATTGAA+GGG - chr2_2:51847726-51847745 None:intergenic 10.0%
!! TTCAATTAAAATACTTATTT+GGG + chr2_2:51847726-51847745 Msa0256790:intron 10.0%
!!! TTTTTCTTAAAAAAATTGAA+GGG - chr2_2:51848361-51848380 None:intergenic 10.0%
!!! ATTTTTCTTAAAAAAATTGA+AGG - chr2_2:51848362-51848381 None:intergenic 10.0%
!! GGTAAAAAATAAAATAAATT+GGG - chr2_2:51848806-51848825 None:intergenic 10.0%
!! TGGTAAAAAATAAAATAAAT+TGG - chr2_2:51848807-51848826 None:intergenic 10.0%
!! CTATTTATAAGAAGAATATT+TGG - chr2_2:51845216-51845235 None:intergenic 15.0%
!! CAAATATTCTTCTTATAAAT+AGG + chr2_2:51845214-51845233 Msa0256790:intron 15.0%
!!! TAAGCAAAAATCAATTTTTT+AGG - chr2_2:51845366-51845385 None:intergenic 15.0%
!!! TGTGAATTTTGTTATTATAT+TGG + chr2_2:51845553-51845572 Msa0256790:intron 15.0%
!!! TTAAAATTAGTCATCTTTTT+AGG + chr2_2:51845760-51845779 Msa0256790:intron 15.0%
!! AATTTAAAAATATCACTTCT+AGG + chr2_2:51845941-51845960 Msa0256790:intron 15.0%
!! AAAAAATTAAGGGATATTTA+TGG - chr2_2:51846071-51846090 None:intergenic 15.0%
!! TAAATAGACTTATTTGAATT+TGG - chr2_2:51847542-51847561 None:intergenic 15.0%
!!! CAAAAAAAAACTCTTTTTAT+TGG + chr2_2:51847611-51847630 Msa0256790:intron 15.0%
!!! CAAATAAGTATTTTAATTGA+AGG - chr2_2:51847727-51847746 None:intergenic 15.0%
!! CTTCAATTAAAATACTTATT+TGG + chr2_2:51847725-51847744 Msa0256790:intron 15.0%
!! GAATATCAAAATGTAATAAT+AGG - chr2_2:51848517-51848536 None:intergenic 15.0%
!!! TATTTTATTTTTTACCAAAG+AGG + chr2_2:51848810-51848829 Msa0256790:intron 15.0%
!!! ATTTTATTTTTTACCAAAGA+GGG + chr2_2:51848811-51848830 Msa0256790:intron 15.0%
!! TGCATTATTATTATTATTCT+AGG + chr2_2:51848966-51848985 Msa0256790:intron 15.0%
!! GATAAAGATGTTAAAGAAAA+AGG - chr2_2:51844870-51844889 None:intergenic 20.0%
!! TATTACATTGTAAATACCAT+TGG + chr2_2:51844962-51844981 Msa0256790:intron 20.0%
!! TATCTTATAAAAAGAACCAA+TGG - chr2_2:51844981-51845000 None:intergenic 20.0%
!! TATGTAAATAATACTCCATT+CGG + chr2_2:51845107-51845126 Msa0256790:intron 20.0%
!! CATTGAGTAAATAATGTATT+TGG - chr2_2:51845430-51845449 None:intergenic 20.0%
!! CCATTATTAAATACTTTATC+CGG - chr2_2:51845487-51845506 None:intergenic 20.0%
!! TAAGAGAAGTAAAATATTGT+GGG - chr2_2:51845523-51845542 None:intergenic 20.0%
!! ATAAGAGAAGTAAAATATTG+TGG - chr2_2:51845524-51845543 None:intergenic 20.0%
!!! AAAGATGACTAATTTTAAGT+GGG - chr2_2:51845758-51845777 None:intergenic 20.0%
!!! AAAAGATGACTAATTTTAAG+TGG - chr2_2:51845759-51845778 None:intergenic 20.0%
!! GAAAAGATTCAAAAACTAAA+AGG + chr2_2:51845858-51845877 Msa0256790:intron 20.0%
!! TCCTTAAAAAAGAAAAAACT+AGG + chr2_2:51846168-51846187 Msa0256790:intron 20.0%
!!! ACCTAGTTTTTTCTTTTTTA+AGG - chr2_2:51846172-51846191 None:intergenic 20.0%
!! TTATAGTTTCCATAGTATAT+TGG - chr2_2:51847170-51847189 None:intergenic 20.0%
!! TTGGAATATAACAAATTTAG+AGG - chr2_2:51847523-51847542 None:intergenic 20.0%
!!! CACAACATATTTTTTTTGAA+TGG - chr2_2:51847580-51847599 None:intergenic 20.0%
!!! TTAAAATACTTATTTGGGTT+CGG + chr2_2:51847731-51847750 Msa0256790:intron 20.0%
!!! CAACATCTTTTAAATTTACA+AGG - chr2_2:51847758-51847777 None:intergenic 20.0%
!! AACACTATTATATTAACTAG+TGG + chr2_2:51848167-51848186 Msa0256790:CDS 20.0%
!! ATTAATCTTGCTAAACAAAA+TGG - chr2_2:51848301-51848320 None:intergenic 20.0%
!! TAATTATGTCACACATATTA+TGG - chr2_2:51848325-51848344 None:intergenic 20.0%
!!! ATGAATCTTGATAATTCATT+TGG + chr2_2:51848380-51848399 Msa0256790:intron 20.0%
!!! TAATTCATTTGGAGTTTAAT+AGG + chr2_2:51848391-51848410 Msa0256790:intron 20.0%
!!! AATAATAGGCTACTTATTTT+TGG - chr2_2:51848503-51848522 None:intergenic 20.0%
!!! CATTTTGATATTCTTTATGT+TGG + chr2_2:51848524-51848543 Msa0256790:intron 20.0%
!!! GAAAAAGATGAACTTTTTTG+TGG - chr2_2:51844533-51844552 None:intergenic 25.0%
!!! TTCATCTTTTTCATTGAGTA+GGG + chr2_2:51844541-51844560 Msa0256790:CDS 25.0%
! ATCCATAAATAATTACCTGA+GGG - chr2_2:51844904-51844923 None:intergenic 25.0%
! TGAGTTAACAAAAAATTACG+TGG + chr2_2:51845005-51845024 Msa0256790:intron 25.0%
! TTGTCTCGTATAAAAAGAAT+TGG + chr2_2:51845068-51845087 Msa0256790:intron 25.0%
! CCAAACTTTCTCTTATAAAT+AGG - chr2_2:51845134-51845153 None:intergenic 25.0%
! CCTATTTATAAGAGAAAGTT+TGG + chr2_2:51845131-51845150 Msa0256790:intron 25.0%
! TTCTTATAAATAGGACAAGA+GGG + chr2_2:51845223-51845242 Msa0256790:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + chr2_2:51845465-51845484 Msa0256790:intron 25.0%
!! CCGGATAAAGTATTTAATAA+TGG + chr2_2:51845484-51845503 Msa0256790:intron 25.0%
!! AAAACTAAAAGGACTTGTTT+GGG - chr2_2:51845869-51845888 None:intergenic 25.0%
!! AAAAACTAAAAGGACTTGTT+TGG - chr2_2:51845868-51845887 None:intergenic 25.0%
! GAAACGATTCAAAAACTAAA+AGG - chr2_2:51845656-51845675 None:intergenic 25.0%
!! TTTCAAACAAGTCTTTTTAG+CGG - chr2_2:51845842-51845861 None:intergenic 25.0%
!! AAAAACTAAAAGGACTTGTT+TGG + chr2_2:51845868-51845887 Msa0256790:intron 25.0%
!! AAAACTAAAAGGACTTGTTT+GGG + chr2_2:51845869-51845888 Msa0256790:intron 25.0%
!! ATGCATAACTTTATGTGTTT+TGG + chr2_2:51846341-51846360 Msa0256790:intron 25.0%
! AACAAAGTTGAGTATTTGTA+TGG - chr2_2:51846484-51846503 None:intergenic 25.0%
!! ATTTTGTCTATGTAGATGTT+TGG + chr2_2:51846963-51846982 Msa0256790:intron 25.0%
! TACTATATATATGGTGAAAC+AGG + chr2_2:51847034-51847053 Msa0256790:intron 25.0%
!! ATGAATTTGGAGTTTATGAA+TGG + chr2_2:51847276-51847295 Msa0256790:CDS 25.0%
!! CACATTTTACCGTAATATAA+AGG - chr2_2:51847460-51847479 None:intergenic 25.0%
! CTTTATATTACGGTAAAATG+TGG + chr2_2:51847458-51847477 Msa0256790:intron 25.0%
! CAACAAATCTTCAATTTAAG+AGG + chr2_2:51847858-51847877 Msa0256790:intron 25.0%
! ATTCATTTGAGAGAAAAAGT+TGG - chr2_2:51847937-51847956 None:intergenic 25.0%
!!! TCATTCTCACAAAAGTTTTT+TGG + chr2_2:51848083-51848102 Msa0256790:intron 25.0%
! AAGGTACGTAATAATTAACA+AGG + chr2_2:51848224-51848243 Msa0256790:intron 25.0%
! TAATTAACAAGGACTTACTT+AGG + chr2_2:51848235-51848254 Msa0256790:intron 25.0%
!! ATATTCTTTATGTTGGTGAA+TGG + chr2_2:51848531-51848550 Msa0256790:intron 25.0%
!! AATGCTTCAAAATCTTGTTT+AGG + chr2_2:51848844-51848863 Msa0256790:intron 25.0%
!! TTTTCAACATATGGATGAAT+TGG + chr2_2:51848874-51848893 Msa0256790:CDS 25.0%
!! AATATGTGACGCAAAATTTT+AGG + chr2_2:51849281-51849300 Msa0256790:CDS 25.0%
! CAAATGTGTACCTAAAATTA+GGG - chr2_2:51849418-51849437 None:intergenic 25.0%
AAAACTTGAGTGATGTTGAT+CGG - chr2_2:51844354-51844373 None:intergenic 30.0%
!! GTTCATCTTTTTCATTGAGT+AGG + chr2_2:51844540-51844559 Msa0256790:CDS 30.0%
! ATGGAATAGAGTTTTTCTTC+TGG + chr2_2:51844598-51844617 Msa0256790:CDS 30.0%
!!! TGTATGTGTCTGTTTTTAGA+TGG + chr2_2:51844683-51844702 Msa0256790:intron 30.0%
!! TGTTACATCTCTGGAATATT+TGG + chr2_2:51844750-51844769 Msa0256790:intron 30.0%
! GTTAAAGAAAAAGGCTTTGT+TGG - chr2_2:51844861-51844880 None:intergenic 30.0%
GATCCATAAATAATTACCTG+AGG - chr2_2:51844905-51844924 None:intergenic 30.0%
TCTCGTATAAAAAGAATTGG+AGG + chr2_2:51845071-51845090 Msa0256790:intron 30.0%
CTTCTTATAAATAGGACAAG+AGG + chr2_2:51845222-51845241 Msa0256790:intron 30.0%
TATTGGTCAAATGCATCTAA+AGG + chr2_2:51845570-51845589 Msa0256790:intron 30.0%
!!! TTGGGACTTTTGAAACTTAA+AGG - chr2_2:51845887-51845906 None:intergenic 30.0%
CCAATTATGCTATCTACAAT+AGG - chr2_2:51845687-51845706 None:intergenic 30.0%
CCTATTGTAGATAGCATAAT+TGG + chr2_2:51845684-51845703 Msa0256790:intron 30.0%
!! ACTAATTTTAAGTGGGTGTT+AGG - chr2_2:51845751-51845770 None:intergenic 30.0%
!! ATTAGTCATCTTTTTAGGCA+TGG + chr2_2:51845765-51845784 Msa0256790:intron 30.0%
!!! TTGGGACTTTTGAAACTTAA+AGG + chr2_2:51845887-51845906 Msa0256790:intron 30.0%
! TGAAACTTAAAGGACTTGTT+TGG + chr2_2:51845897-51845916 Msa0256790:intron 30.0%
! GAAACTTAAAGGACTTGTTT+GGG + chr2_2:51845898-51845917 Msa0256790:intron 30.0%
AAGGGATATTTATGGTGAAT+GGG - chr2_2:51846063-51846082 None:intergenic 30.0%
TAAGGGATATTTATGGTGAA+TGG - chr2_2:51846064-51846083 None:intergenic 30.0%
TTTAAGGATCATGACAAAGT+AGG - chr2_2:51846156-51846175 None:intergenic 30.0%
! TTGTTTTCATACATGCAGAT+TGG + chr2_2:51846248-51846267 Msa0256790:intron 30.0%
! AGCTTATGATTACACACTTT+TGG + chr2_2:51846422-51846441 Msa0256790:intron 30.0%
! TGTTTTGCAGATTGTGTTAT+GGG + chr2_2:51846665-51846684 Msa0256790:intron 30.0%
!!! TTTTAGGAAGGTATTGAAAC+AGG - chr2_2:51846758-51846777 None:intergenic 30.0%
CCTTCCTAAAATTTATCACT+CGG + chr2_2:51846767-51846786 Msa0256790:CDS 30.0%
CAAAAATGAAACCATTTGTG+AGG - chr2_2:51846812-51846831 None:intergenic 30.0%
! CTCACAAATGGTTTCATTTT+TGG + chr2_2:51846810-51846829 Msa0256790:CDS 30.0%
GTAATCGTTAGTACTTACAT+GGG - chr2_2:51846886-51846905 None:intergenic 30.0%
AGTAATCGTTAGTACTTACA+TGG - chr2_2:51846887-51846906 None:intergenic 30.0%
TCTACATAGACAAAATTGCA+GGG - chr2_2:51846959-51846978 None:intergenic 30.0%
ATCTACATAGACAAAATTGC+AGG - chr2_2:51846960-51846979 None:intergenic 30.0%
TATGTAGATGTTTGGATTGA+CGG + chr2_2:51846971-51846990 Msa0256790:intron 30.0%
CGGTTAAATTGTCAAAATCA+TGG + chr2_2:51846991-51847010 Msa0256790:intron 30.0%
TGCAAAGAACCAATATACTA+TGG + chr2_2:51847158-51847177 Msa0256790:CDS 30.0%
TGAAGAAATTAGACAGAACA+AGG + chr2_2:51847195-51847214 Msa0256790:CDS 30.0%
!! AGTGCTGATAACTATGAATT+TGG + chr2_2:51847263-51847282 Msa0256790:CDS 30.0%
!!! GAAGGAGTATCTTTTCAATT+CGG - chr2_2:51847328-51847347 None:intergenic 30.0%
ATGAATCCATTATACCCAAT+TGG + chr2_2:51847951-51847970 Msa0256790:intron 30.0%
TGAATTCCAATTGGGTATAA+TGG - chr2_2:51847960-51847979 None:intergenic 30.0%
TGTCATCATGAATTCCAATT+GGG - chr2_2:51847968-51847987 None:intergenic 30.0%
ATGTCATCATGAATTCCAAT+TGG - chr2_2:51847969-51847988 None:intergenic 30.0%
AATAATGTGAATCACGTACA+TGG - chr2_2:51848016-51848035 None:intergenic 30.0%
GATTCACATTATTTGTACGT+TGG + chr2_2:51848023-51848042 Msa0256790:intron 30.0%
TATGCTAATTGGAAACATCA+AGG + chr2_2:51848205-51848224 Msa0256790:CDS 30.0%
GAAATTGAAGATGACAAACA+TGG + chr2_2:51848595-51848614 Msa0256790:CDS 30.0%
!! TCAGTTGATAAAAAATCTCC+CGG + chr2_2:51848718-51848737 Msa0256790:CDS 30.0%
TATCAAGTACCATCTTTGTT+TGG + chr2_2:51848757-51848776 Msa0256790:CDS 30.0%
! ATCAAGTACCATCTTTGTTT+GGG + chr2_2:51848758-51848777 Msa0256790:CDS 30.0%
ATTAACATTCAAACCCTCTT+TGG - chr2_2:51848827-51848846 None:intergenic 30.0%
TCATCCATATGTTGAAAAAG+TGG - chr2_2:51848872-51848891 None:intergenic 30.0%
! TAGAATGCTATTTGTGGTAA+GGG + chr2_2:51849063-51849082 Msa0256790:CDS 30.0%
!! AACTTTTAACTCCATCTCTT+AGG - chr2_2:51849111-51849130 None:intergenic 30.0%
GTTAAAAGTTGTTGTATGCT+AGG + chr2_2:51849121-51849140 Msa0256790:CDS 30.0%
GCAAATGTGTACCTAAAATT+AGG - chr2_2:51849419-51849438 None:intergenic 30.0%
TGAGTTCTTTGTCCTTTATT+CGG + chr2_2:51849546-51849565 Msa0256790:CDS 30.0%
!! AAATCATCTTGATTTGGCTT+TGG - chr2_2:51849647-51849666 None:intergenic 30.0%
GAGTCAAAATCATCTTGATT+TGG - chr2_2:51849653-51849672 None:intergenic 30.0%
AAACTTGAGTGATGTTGATC+GGG - chr2_2:51844353-51844372 None:intergenic 35.0%
!!! TGAACTTTTTTGTGGCTGTT+TGG - chr2_2:51844525-51844544 None:intergenic 35.0%
TACTTGATCCAAGAGGAAAA+TGG + chr2_2:51844567-51844586 Msa0256790:CDS 35.0%
ACTTGATCCAAGAGGAAAAT+GGG + chr2_2:51844568-51844587 Msa0256790:CDS 35.0%
AGAGTGCATAGAAGAAAAGT+GGG - chr2_2:51844649-51844668 None:intergenic 35.0%
!!! TGTGTCTGTTTTTAGATGGT+TGG + chr2_2:51844687-51844706 Msa0256790:intron 35.0%
! AAACATAAACGCTCCTCTTT+TGG + chr2_2:51844791-51844810 Msa0256790:CDS 35.0%
ATGATGAATGAAGCCAAAAG+AGG - chr2_2:51844807-51844826 None:intergenic 35.0%
!!! TCTTTTGGCTTCATTCATCA+TGG + chr2_2:51844806-51844825 Msa0256790:CDS 35.0%
AAATAATTACCTGAGGGAGA+GGG - chr2_2:51844898-51844917 None:intergenic 35.0%
TAAATAATTACCTGAGGGAG+AGG - chr2_2:51844899-51844918 None:intergenic 35.0%
CTCCCTCAGGTAATTATTTA+TGG + chr2_2:51844899-51844918 Msa0256790:intron 35.0%
CTCTTATAAATAGGACCGAA+TGG - chr2_2:51845125-51845144 None:intergenic 35.0%
!! GTTTTTCGTTTTTCCCAAAG+TGG + chr2_2:51845600-51845619 Msa0256790:intron 35.0%
GAAACTTAAAGGACCACTTT+GGG - chr2_2:51845616-51845635 None:intergenic 35.0%
TGAAACTTAAAGGACCACTT+TGG - chr2_2:51845617-51845636 None:intergenic 35.0%
!! ATCTTTTTAGGCATGGCAAA+TGG + chr2_2:51845772-51845791 Msa0256790:intron 35.0%
CATGTTGCATAATACTGAGT+TGG + chr2_2:51846195-51846214 Msa0256790:intron 35.0%
! AATACTGAGTTGGCAAATGA+AGG + chr2_2:51846205-51846224 Msa0256790:intron 35.0%
TGAAGCTATAAGTGTAATGC+AGG - chr2_2:51846284-51846303 None:intergenic 35.0%
! GACATAAGATGTGTACGTTT+TGG + chr2_2:51846363-51846382 Msa0256790:intron 35.0%
!! CACACTTTTGGAAAACTTGT+TGG + chr2_2:51846434-51846453 Msa0256790:intron 35.0%
! AATGTCTGATTGCTGATTAG+AGG - chr2_2:51846460-51846479 None:intergenic 35.0%
! CTGTTTTGCAGATTGTGTTA+TGG + chr2_2:51846664-51846683 Msa0256790:intron 35.0%
ATTCCATCTTTGTTAGAGCA+AGG + chr2_2:51846693-51846712 Msa0256790:CDS 35.0%
!! CCGAGTGATAAATTTTAGGA+AGG - chr2_2:51846770-51846789 None:intergenic 35.0%
! CAGACCGAGTGATAAATTTT+AGG - chr2_2:51846774-51846793 None:intergenic 35.0%
!!! GTTTCATTTTTGGAACCGTT+TGG + chr2_2:51846820-51846839 Msa0256790:CDS 35.0%
GCAGGGTTTAACAACATTTA+TGG - chr2_2:51846942-51846961 None:intergenic 35.0%
! GTATTTGTTGGGACTTCAAA+GGG + chr2_2:51847073-51847092 Msa0256790:CDS 35.0%
GAAATTAGACAGAACAAGGT+TGG + chr2_2:51847199-51847218 Msa0256790:CDS 35.0%
! AGATACTCCTTCCTATCTTT+TGG + chr2_2:51847336-51847355 Msa0256790:CDS 35.0%
! GATACTCCTTCCTATCTTTT+GGG + chr2_2:51847337-51847356 Msa0256790:CDS 35.0%
! ATACTCCTTCCTATCTTTTG+GGG + chr2_2:51847338-51847357 Msa0256790:CDS 35.0%
GAAAATACCTGAGAGTCATT+AGG - chr2_2:51847364-51847383 None:intergenic 35.0%
CGCTGCAAACCTTTATATTA+CGG + chr2_2:51847448-51847467 Msa0256790:intron 35.0%
CGGTAAAATGTGGATAATCA+CGG + chr2_2:51847468-51847487 Msa0256790:intron 35.0%
GTGGATAATCACGGTATTTA+CGG + chr2_2:51847477-51847496 Msa0256790:intron 35.0%
TACATGACCCAACATAATCA+CGG - chr2_2:51847818-51847837 None:intergenic 35.0%
! TTGTACGTTGGTTGAAGTTA+AGG + chr2_2:51848035-51848054 Msa0256790:intron 35.0%
CAATTAGCATAGTGACTAGA+AGG - chr2_2:51848196-51848215 None:intergenic 35.0%
CTTCTAGTCACTATGCTAAT+TGG + chr2_2:51848194-51848213 Msa0256790:CDS 35.0%
!! TTTTGGCTGGTCAAATTGAT+CGG - chr2_2:51848486-51848505 None:intergenic 35.0%
!!! ATAGGCTACTTATTTTTGGC+TGG - chr2_2:51848499-51848518 None:intergenic 35.0%
! TCTTTATGTTGGTGAATGGT+AGG + chr2_2:51848535-51848554 Msa0256790:intron 35.0%
TTGATAAAAAATCTCCCGGA+GGG + chr2_2:51848722-51848741 Msa0256790:CDS 35.0%
!!! GGTACCACTTTTTCAACATA+TGG + chr2_2:51848865-51848884 Msa0256790:intron 35.0%
AAGTCCCTTGTATTCACAAA+GGG + chr2_2:51848923-51848942 Msa0256790:CDS 35.0%
TTCTCCCTTTGTGAATACAA+GGG - chr2_2:51848930-51848949 None:intergenic 35.0%
TTTCTCCCTTTGTGAATACA+AGG - chr2_2:51848931-51848950 None:intergenic 35.0%
TGCATACAGTTAACAAGAGA+AGG + chr2_2:51849028-51849047 Msa0256790:intron 35.0%
CAAATAGCATTCTACGAACT+GGG - chr2_2:51849057-51849076 None:intergenic 35.0%
ACAAATAGCATTCTACGAAC+TGG - chr2_2:51849058-51849077 None:intergenic 35.0%
! AGTTCGTAGAATGCTATTTG+TGG + chr2_2:51849057-51849076 Msa0256790:CDS 35.0%
! GTAGAATGCTATTTGTGGTA+AGG + chr2_2:51849062-51849081 Msa0256790:CDS 35.0%
! AGAATGCTATTTGTGGTAAG+GGG + chr2_2:51849064-51849083 Msa0256790:CDS 35.0%
! TTAGGAATTGGCTACTTTGA+AGG - chr2_2:51849093-51849112 None:intergenic 35.0%
TAACTCCATCTCTTAGGAAT+TGG - chr2_2:51849105-51849124 None:intergenic 35.0%
GTTGGAAGACGTTCTATAAA+TGG - chr2_2:51849196-51849215 None:intergenic 35.0%
! CTTACAACAGTCGAAGTATT+TGG + chr2_2:51849238-51849257 Msa0256790:CDS 35.0%
! AACAAGTGTGCCCTAATTTT+AGG + chr2_2:51849405-51849424 Msa0256790:intron 35.0%
AGTGTTAGATATTACTCACC+TGG + chr2_2:51849458-51849477 Msa0256790:CDS 35.0%
TTAGATATTACTCACCTGGT+TGG + chr2_2:51849462-51849481 Msa0256790:CDS 35.0%
TCTTCATAACATCGTGTTTG+TGG + chr2_2:51849671-51849690 Msa0256790:exon 35.0%
AACTTGAGTGATGTTGATCG+GGG - chr2_2:51844352-51844371 None:intergenic 40.0%
! AGGGTATTACTTGATCCAAG+AGG + chr2_2:51844560-51844579 Msa0256790:CDS 40.0%
! TTTTCTTCTGGTGTGTGCAA+TGG + chr2_2:51844610-51844629 Msa0256790:intron 40.0%
TTTCTTCTGGTGTGTGCAAT+GGG + chr2_2:51844611-51844630 Msa0256790:intron 40.0%
! GTGTGCAATGGGTTTATTTG+TGG + chr2_2:51844622-51844641 Msa0256790:intron 40.0%
! GAGAGTGCATAGAAGAAAAG+TGG - chr2_2:51844650-51844669 None:intergenic 40.0%
TGCTTCCTTGAACTCATCTT+TGG + chr2_2:51845711-51845730 Msa0256790:intron 40.0%
TGGTACCAAAGATGAGTTCA+AGG - chr2_2:51845719-51845738 None:intergenic 40.0%
CTCATCTTTGGTACCAAATC+TGG + chr2_2:51845723-51845742 Msa0256790:intron 40.0%
!! TTTTTAGGCATGGCAAATGG+TGG + chr2_2:51845775-51845794 Msa0256790:intron 40.0%
!! CAAGTCTTTTTAGCGGATGA+TGG - chr2_2:51845835-51845854 None:intergenic 40.0%
GATCCTTGCTCTAACAAAGA+TGG - chr2_2:51846699-51846718 None:intergenic 40.0%
!!! TCATTTTTGGAACCGTTTGG+TGG + chr2_2:51846823-51846842 Msa0256790:CDS 40.0%
!!! CATTTTTGGAACCGTTTGGT+GGG + chr2_2:51846824-51846843 Msa0256790:CDS 40.0%
!!! ATTTTTGGAACCGTTTGGTG+GGG + chr2_2:51846825-51846844 Msa0256790:CDS 40.0%
AGGGTCATGTTGGTATTTGT+TGG + chr2_2:51847061-51847080 Msa0256790:CDS 40.0%
GGGTCATGTTGGTATTTGTT+GGG + chr2_2:51847062-51847081 Msa0256790:CDS 40.0%
! GGTATTTGTTGGGACTTCAA+AGG + chr2_2:51847072-51847091 Msa0256790:CDS 40.0%
! TAGACAGAACAAGGTTGGTT+TGG + chr2_2:51847204-51847223 Msa0256790:CDS 40.0%
GTAGTTGCGATGATGACTAA+TGG + chr2_2:51847416-51847435 Msa0256790:intron 40.0%
CGGTTGAAATATCATTGCAG+AGG + chr2_2:51847497-51847516 Msa0256790:intron 40.0%
! GTGATGTTGTCAGCACTTTT+GGG + chr2_2:51848105-51848124 Msa0256790:intron 40.0%
TTAGCATAGTGACTAGAAGG+AGG - chr2_2:51848193-51848212 None:intergenic 40.0%
TTATGCTAGAGATCGAACGT+AGG + chr2_2:51848450-51848469 Msa0256790:intron 40.0%
! TTTGGCTGGTCAAATTGATC+GGG - chr2_2:51848485-51848504 None:intergenic 40.0%
TGAAGATGACAAACATGGAG+TGG + chr2_2:51848600-51848619 Msa0256790:CDS 40.0%
!! AGTGGTGGATGAACAAAAGA+CGG + chr2_2:51848618-51848637 Msa0256790:CDS 40.0%
TAACTACGAGAGACAGTGTT+GGG + chr2_2:51848670-51848689 Msa0256790:CDS 40.0%
! GTTGATAAAAAATCTCCCGG+AGG + chr2_2:51848721-51848740 Msa0256790:CDS 40.0%
! GTACCATCTTTGTTTGGGTT+TGG + chr2_2:51848763-51848782 Msa0256790:CDS 40.0%
TCACCAAACCCAAACAAAGA+TGG - chr2_2:51848769-51848788 None:intergenic 40.0%
CAAGTCCCTTGTATTCACAA+AGG + chr2_2:51848922-51848941 Msa0256790:CDS 40.0%
AGTAGCCAATTCCTAAGAGA+TGG + chr2_2:51849097-51849116 Msa0256790:CDS 40.0%
GTGGAGATGAACTTCTATCA+TGG + chr2_2:51849158-51849177 Msa0256790:CDS 40.0%
!! TCCAACATCATCATCGACTT+TGG + chr2_2:51849210-51849229 Msa0256790:CDS 40.0%
! ACCAAAGTCGATGATGATGT+TGG - chr2_2:51849214-51849233 None:intergenic 40.0%
AGTATTTGGACTTGAAGCAG+AGG + chr2_2:51849252-51849271 Msa0256790:CDS 40.0%
!! CAATTCAATTGGCTTGGAGA+AGG + chr2_2:51849504-51849523 Msa0256790:CDS 40.0%
!! AATTGGCTTGGAGAAGGTAT+AGG + chr2_2:51849510-51849529 Msa0256790:CDS 40.0%
AAAGAAGTGGACCTTGACAA+AGG - chr2_2:51849581-51849600 None:intergenic 40.0%
TTTGTCAAGGTCCACTTCTT+TGG + chr2_2:51849580-51849599 Msa0256790:CDS 40.0%
TCTATCCTCTTCCAAAGAAG+TGG - chr2_2:51849594-51849613 None:intergenic 40.0%
!! ATCTTGATTTGGCTTTGGTG+AGG - chr2_2:51849642-51849661 None:intergenic 40.0%
ACTTGAGTGATGTTGATCGG+GGG - chr2_2:51844351-51844370 None:intergenic 45.0%
! TCTTCAGCCCATTTTCCTCT+TGG - chr2_2:51844578-51844597 None:intergenic 45.0%
!! GAGGAAAATGGGCTGAAGAA+TGG + chr2_2:51844579-51844598 Msa0256790:CDS 45.0%
GTAACATGTCCGTCATGCAA+CGG - chr2_2:51844736-51844755 None:intergenic 45.0%
! TGACGGACATGTTACATCTC+TGG + chr2_2:51844741-51844760 Msa0256790:intron 45.0%
!! GGCTTTGTTGGCTTTGAAGA+AGG - chr2_2:51844849-51844868 None:intergenic 45.0%
! AGCGGATGATGGTGATGATT+CGG - chr2_2:51845824-51845843 None:intergenic 45.0%
TAGAGCAAGGATCAATTGCG+CGG + chr2_2:51846706-51846725 Msa0256790:CDS 45.0%
TATATGGTGAAACAGGCAGC+AGG + chr2_2:51847041-51847060 Msa0256790:intron 45.0%
ATATGGTGAAACAGGCAGCA+GGG + chr2_2:51847042-51847061 Msa0256790:intron 45.0%
TTAGGCCCCAAAAGATAGGA+AGG - chr2_2:51847346-51847365 None:intergenic 45.0%
GTCATTAGGCCCCAAAAGAT+AGG - chr2_2:51847350-51847369 None:intergenic 45.0%
GCGATGATGACTAATGGCTA+TGG + chr2_2:51847422-51847441 Msa0256790:intron 45.0%
GGCTCAATAAGCTCACAGTA+GGG - chr2_2:51847789-51847808 None:intergenic 45.0%
TGGCTCAATAAGCTCACAGT+AGG - chr2_2:51847790-51847809 None:intergenic 45.0%
CACGTGACCGTGATTATGTT+GGG + chr2_2:51847808-51847827 Msa0256790:intron 45.0%
! GGTGATGTTGTCAGCACTTT+TGG + chr2_2:51848104-51848123 Msa0256790:intron 45.0%
ACCTCCAGAGTACAACAGAA+AGG + chr2_2:51848129-51848148 Msa0256790:CDS 45.0%
TCCTTTCTGTTGTACTCTGG+AGG - chr2_2:51848133-51848152 None:intergenic 45.0%
CCATCCTTTCTGTTGTACTC+TGG - chr2_2:51848136-51848155 None:intergenic 45.0%
CCAGAGTACAACAGAAAGGA+TGG + chr2_2:51848133-51848152 Msa0256790:CDS 45.0%
CTGGTCAAATTGATCGGGAA+CGG - chr2_2:51848480-51848499 None:intergenic 45.0%
AGATGACAAACATGGAGTGG+TGG + chr2_2:51848603-51848622 Msa0256790:CDS 45.0%
AACAAAAGACGGTTGCCACA+AGG + chr2_2:51848629-51848648 Msa0256790:CDS 45.0%
GTAACTACGAGAGACAGTGT+TGG + chr2_2:51848669-51848688 Msa0256790:CDS 45.0%
ATATCCCTTCTCAAACCCTC+CGG - chr2_2:51848740-51848759 None:intergenic 45.0%
!! TTGTTTGGGTTTGGTGAGAC+AGG + chr2_2:51848772-51848791 Msa0256790:CDS 45.0%
AGTTGTTGTATGCTAGGACC+AGG + chr2_2:51849127-51849146 Msa0256790:CDS 45.0%
TCATCTCCACTGAAGTTTCC+TGG - chr2_2:51849148-51849167 None:intergenic 45.0%
CTGTGGCAATTCAATTGGCT+TGG + chr2_2:51849498-51849517 Msa0256790:CDS 45.0%
! CTTGGAGAAGGTATAGGCAT+AGG + chr2_2:51849516-51849535 Msa0256790:CDS 45.0%
! AAGGTCCACTTCTTTGGAAG+AGG + chr2_2:51849586-51849605 Msa0256790:CDS 45.0%
!! AAGTAAAATAAAAAAATTAA+GGG - chr2_2:51846081-51846100 None:intergenic 5.0%
!! AAAGTAAAATAAAAAAATTA+AGG - chr2_2:51846082-51846101 None:intergenic 5.0%
ATCGTCGATGTCATCGTCAG+TGG - chr2_2:51844408-51844427 None:intergenic 50.0%
CGACGATATCGTCAACAACG+AGG + chr2_2:51844421-51844440 Msa0256790:CDS 50.0%
! CATGCAACGGATAACGGTGA+CGG - chr2_2:51844723-51844742 None:intergenic 50.0%
CACCGTTATCCGTTGCATGA+CGG + chr2_2:51844724-51844743 Msa0256790:intron 50.0%
GTCCGTCATGCAACGGATAA+CGG - chr2_2:51844729-51844748 None:intergenic 50.0%
TAATTACCTGAGGGAGAGGG+AGG - chr2_2:51844895-51844914 None:intergenic 50.0%
TGGGTGTTAGGAGCCAGATT+TGG - chr2_2:51845739-51845758 None:intergenic 50.0%
AGAGCAAGGATCAATTGCGC+GGG + chr2_2:51846707-51846726 Msa0256790:CDS 50.0%
GTGCGTCGAAACCTCACAAA+TGG + chr2_2:51846798-51846817 Msa0256790:CDS 50.0%
GATAGCGAATCCCCACCAAA+CGG - chr2_2:51846838-51846857 None:intergenic 50.0%
GAGCAATGTGAGACAACACC+TGG + chr2_2:51847113-51847132 Msa0256790:CDS 50.0%
TGTGAGACAACACCTGGTTG+TGG + chr2_2:51847119-51847138 Msa0256790:CDS 50.0%
GTGAGACAACACCTGGTTGT+GGG + chr2_2:51847120-51847139 Msa0256790:CDS 50.0%
AGTGTTCTTAGCCCACAACC+AGG - chr2_2:51847134-51847153 None:intergenic 50.0%
GGTCACAGAACACAGAAGCA+AGG + chr2_2:51847225-51847244 Msa0256790:CDS 50.0%
! TTTGGGGCCTAATGACTCTC+AGG + chr2_2:51847354-51847373 Msa0256790:CDS 50.0%
CCAACATAATCACGGTCACG+TGG - chr2_2:51847810-51847829 None:intergenic 50.0%
CCACGTGACCGTGATTATGT+TGG + chr2_2:51847807-51847826 Msa0256790:intron 50.0%
TATCCCTTCTCAAACCCTCC+GGG - chr2_2:51848739-51848758 None:intergenic 50.0%
!! TGGGTTTGGTGAGACAGGTA+AGG + chr2_2:51848777-51848796 Msa0256790:intron 50.0%
CTAGGACCAGGAAACTTCAG+TGG + chr2_2:51849139-51849158 Msa0256790:CDS 50.0%
ACTCACCTGGTTGGAGAACT+TGG + chr2_2:51849471-51849490 Msa0256790:CDS 50.0%
CTCACCTGGTTGGAGAACTT+GGG + chr2_2:51849472-51849491 Msa0256790:CDS 50.0%
GGCTGCTGTGGCAATTCAAT+TGG + chr2_2:51849493-51849512 Msa0256790:CDS 50.0%
TGTCCTTTATTCGGCCTCGT+AGG + chr2_2:51849555-51849574 Msa0256790:CDS 50.0%
CGATAGCGACGAGTACGATG+AGG + chr2_2:51844451-51844470 Msa0256790:CDS 55.0%
AACAGGCAGCAGGGTCATGT+TGG + chr2_2:51847051-51847070 Msa0256790:intron 55.0%
GACGGTTGCCACAAGGACTT+CGG + chr2_2:51848636-51848655 Msa0256790:CDS 55.0%
ACATTCATCAACGCCGCGCA+TGG - chr2_2:51848699-51848718 None:intergenic 55.0%
ATCTCCCGGAGGGTTTGAGA+AGG + chr2_2:51848732-51848751 Msa0256790:CDS 55.0%
TCTCCCGGAGGGTTTGAGAA+GGG + chr2_2:51848733-51848752 Msa0256790:CDS 55.0%
TTGGAGAACTTGGGCTGCTG+TGG + chr2_2:51849481-51849500 Msa0256790:CDS 55.0%
AGGCCTACGAGGCCGAATAA+AGG - chr2_2:51849561-51849580 None:intergenic 55.0%
GACCTTGACAAAGGCCTACG+AGG - chr2_2:51849572-51849591 None:intergenic 55.0%
TATCCTCCTCCCTCTCCCTC+AGG + chr2_2:51844886-51844905 Msa0256790:CDS 60.0%
TTACCTGAGGGAGAGGGAGG+AGG - chr2_2:51844892-51844911 None:intergenic 60.0%
ACACGCTGCCGAAGTCCTTG+TGG - chr2_2:51848647-51848666 None:intergenic 60.0%
GCAGCCCAAGTTCTCCAACC+AGG - chr2_2:51849479-51849498 None:intergenic 60.0%
GGCCTCGTAGGCCTTTGTCA+AGG + chr2_2:51849567-51849586 Msa0256790:CDS 60.0%
! CAGTGTTGGGCTGCCATGCG+CGG + chr2_2:51848683-51848702 Msa0256790:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 51844344 51849734 51844344 ID=Msa0256790;Name=Msa0256790
chr2_2 mRNA 51844344 51849734 51844344 ID=Msa0256790-mRNA-1;Parent=Msa0256790;Name=Msa0256790-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.87|0.77|1|1|1|9|55|629
chr2_2 exon 51844344 51844619 51844344 ID=Msa0256790-mRNA-1:exon:15640;Parent=Msa0256790-mRNA-1
chr2_2 exon 51844779 51844907 51844779 ID=Msa0256790-mRNA-1:exon:15641;Parent=Msa0256790-mRNA-1
chr2_2 exon 51846675 51846887 51846675 ID=Msa0256790-mRNA-1:exon:15642;Parent=Msa0256790-mRNA-1
chr2_2 exon 51847056 51847375 51847056 ID=Msa0256790-mRNA-1:exon:15643;Parent=Msa0256790-mRNA-1
chr2_2 exon 51848118 51848226 51848118 ID=Msa0256790-mRNA-1:exon:15644;Parent=Msa0256790-mRNA-1
chr2_2 exon 51848557 51848793 51848557 ID=Msa0256790-mRNA-1:exon:15645;Parent=Msa0256790-mRNA-1
chr2_2 exon 51848866 51848952 51848866 ID=Msa0256790-mRNA-1:exon:15646;Parent=Msa0256790-mRNA-1
chr2_2 exon 51849037 51849302 51849037 ID=Msa0256790-mRNA-1:exon:15647;Parent=Msa0256790-mRNA-1
chr2_2 exon 51849427 51849734 51849427 ID=Msa0256790-mRNA-1:exon:15648;Parent=Msa0256790-mRNA-1
chr2_2 CDS 51844344 51844619 51844344 ID=Msa0256790-mRNA-1:cds;Parent=Msa0256790-mRNA-1
chr2_2 CDS 51844779 51844907 51844779 ID=Msa0256790-mRNA-1:cds;Parent=Msa0256790-mRNA-1
chr2_2 CDS 51846675 51846887 51846675 ID=Msa0256790-mRNA-1:cds;Parent=Msa0256790-mRNA-1
chr2_2 CDS 51847056 51847375 51847056 ID=Msa0256790-mRNA-1:cds;Parent=Msa0256790-mRNA-1
chr2_2 CDS 51848118 51848226 51848118 ID=Msa0256790-mRNA-1:cds;Parent=Msa0256790-mRNA-1
chr2_2 CDS 51848557 51848793 51848557 ID=Msa0256790-mRNA-1:cds;Parent=Msa0256790-mRNA-1
chr2_2 CDS 51848866 51848952 51848866 ID=Msa0256790-mRNA-1:cds;Parent=Msa0256790-mRNA-1
chr2_2 CDS 51849037 51849302 51849037 ID=Msa0256790-mRNA-1:cds;Parent=Msa0256790-mRNA-1
chr2_2 CDS 51849427 51849679 51849427 ID=Msa0256790-mRNA-1:cds;Parent=Msa0256790-mRNA-1
chr2_2 three_prime_UTR 51849680 51849734 51849680 ID=Msa0256790-mRNA-1:three_prime_utr;Parent=Msa0256790-mRNA-1
Gene Sequence

>Msa0256790

ATGGCCCCCGATCAACATCACTCAAGTTTTAATTCAAACATGCAACACTACTATTACAACACCACTGACGATGACATCGACGATATCGTCAACAACGAGGTTGTTAACGATAGCGACGAGTACGATGAGGATGAGTATCAATATCAGTATCAGCATGAAGAAGATGAAAAAGAAGAAACCAAACAGCCACAAAAAAGTTCATCTTTTTCATTGAGTAGGGTATTACTTGATCCAAGAGGAAAATGGGCTGAAGAATGGAATAGAGTTTTTCTTCTGTTCTATATTCATAAACATAAACGCTCCTCTTTTGGCTTCATTCATCATGGAACTTCTTCTTCTGACTCCTTCTTCAAAGCCAACAAAGCCTTTTTCTTTAACATCTTTATCCTCCTCCCTCTCCCTCAGATTGTGTTATGGGTGACAATTCCATCTTTGTTAGAGCAAGGATCAATTGCGCGGGTGATTTCTGTATTGTTGATTATGTTCCTGTTTCAATACCTTCCTAAAATTTATCACTCGGTCTGCTTCGTGCGTCGAAACCTCACAAATGGTTTCATTTTTGGAACCGTTTGGTGGGGATTCGCTATCAATATGATCGCTTATTTTGTCGCTTCCCATGCAGCAGGGTCATGTTGGTATTTGTTGGGACTTCAAAGGGCAGCAAAGTGTCTAGAAGAGCAATGTGAGACAACACCTGGTTGTGGGCTAAGAACACTATGTTGCAAAGAACCAATATACTATGGAAACTATAACATGTTGAAGAAATTAGACAGAACAAGGTTGGTTTGGTCACAGAACACAGAAGCAAGGTCTACATGTTTAGCTAGTGCTGATAACTATGAATTTGGAGTTTATGAATGGAGTGTTCAACTTGTTACAAATAATAGCCGAATTGAAAAGATACTCCTTCCTATCTTTTGGGGCCTAATGACTCTCAGCACTTTTGGGAACCTCCAGAGTACAACAGAAAGGATGGAAGTCGTTTTCAACACTATTATATTAACTAGTGGCCTCCTTCTAGTCACTATGCTAATTGGAAACATCAAGGTATTTTTACATGCTACAACATCAAAGAAGCAAGCAATGAAATTGAAGATGACAAACATGGAGTGGTGGATGAACAAAAGACGGTTGCCACAAGGACTTCGGCAGCGTGTGCGTAACTACGAGAGACAGTGTTGGGCTGCCATGCGCGGCGTTGATGAATGTCAGTTGATAAAAAATCTCCCGGAGGGTTTGAGAAGGGATATCAAGTACCATCTTTGTTTGGGTTTGGTGAGACAGGTACCACTTTTTCAACATATGGATGAATTGGTGCTAGAGTACATTTGTGACTGTGTCAAGTCCCTTGTATTCACAAAGGGAGAAACATTAACAAGAGAAGGAGACCCAGTTCGTAGAATGCTATTTGTGGTAAGGGGTCACCTTCAAAGTAGCCAATTCCTAAGAGATGGAGTTAAAAGTTGTTGTATGCTAGGACCAGGAAACTTCAGTGGAGATGAACTTCTATCATGGTGTTTAAGAAGACCATTTATAGAACGTCTTCCAACATCATCATCGACTTTGGTGACGCTTACAACAGTCGAAGTATTTGGACTTGAAGCAGAGGATGTCAAATATGTGACGCAAAATTTTAGGTACACATTTGCAAAAGAAAATGTTAGAAGAAGTGTTAGATATTACTCACCTGGTTGGAGAACTTGGGCTGCTGTGGCAATTCAATTGGCTTGGAGAAGGTATAGGCATAGGTTGACTTTGAGTTCTTTGTCCTTTATTCGGCCTCGTAGGCCTTTGTCAAGGTCCACTTCTTTGGAAGAGGATAGACTTCGTCTTTATACAGCTTTGCTAACCTCACCAAAGCCAAATCAAGATGATTTTGACTCTTCATAA
Protein sequence

>Msa0256790

MAPDQHHSSFNSNMQHYYYNTTDDDIDDIVNNEVVNDSDEYDEDEYQYQYQHEEDEKEETKQPQKSSSFSLSRVLLDPRGKWAEEWNRVFLLFYIHKHKRSSFGFIHHGTSSSDSFFKANKAFFFNIFILLPLPQIVLWVTIPSLLEQGSIARVISVLLIMFLFQYLPKIYHSVCFVRRNLTNGFIFGTVWWGFAINMIAYFVASHAAGSCWYLLGLQRAAKCLEEQCETTPGCGLRTLCCKEPIYYGNYNMLKKLDRTRLVWSQNTEARSTCLASADNYEFGVYEWSVQLVTNNSRIEKILLPIFWGLMTLSTFGNLQSTTERMEVVFNTIILTSGLLLVTMLIGNIKVFLHATTSKKQAMKLKMTNMEWWMNKRRLPQGLRQRVRNYERQCWAAMRGVDECQLIKNLPEGLRRDIKYHLCLGLVRQVPLFQHMDELVLEYICDCVKSLVFTKGETLTREGDPVRRMLFVVRGHLQSSQFLRDGVKSCCMLGPGNFSGDELLSWCLRRPFIERLPTSSSTLVTLTTVEVFGLEAEDVKYVTQNFRYTFAKENVRRSVRYYSPGWRTWAAVAIQLAWRRYRHRLTLSSLSFIRPRRPLSRSTSLEEDRLRLYTALLTSPKPNQDDFDSS*