AlfalfaGEDB | Msa0263420

M. sativa cultivar zhongmu-4 / Msa0263420

KEGG: -
GO: -
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263420	QDX01706.1	100.000	312	0	0	2	313	1	312	0.0	643

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263420	sp\|O49255\|NAC29_ARATH	66.471	170	55	1	11	180	5	172	4.42e-80	247

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263420	A0A6B7JGD8	100.000	312	0	0	2	313	1	312	0.0	643

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TFs/TRs:

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Gene ID	Type	Classification
Msa0263420	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0263420	Msa0338560	0.959769	8.280266e-118	-8.615850e-47
Msa0263420	Msa0909140	0.800958	1.128633e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263420	MtrunA17_Chr2g0326361	93.396	318	21	0	2	319	1	318	0.0	610
Msa0263420	MtrunA17_Chr7g0263931	48.024	329	141	5	11	319	7	325	2.42e-94	283
Msa0263420	MtrunA17_Chr5g0417791	67.059	170	54	1	15	184	3	170	7.43e-83	251
Msa0263420	MtrunA17_Chr4g0042561	67.052	173	55	1	9	181	3	173	4.22e-82	249
Msa0263420	MtrunA17_Chr2g0318101	48.810	252	84	6	15	259	15	228	9.28e-80	246
Msa0263420	MtrunA17_Chr7g0252971	66.286	175	50	4	15	181	8	181	6.12e-79	244
Msa0263420	MtrunA17_Chr4g0047841	63.842	177	45	2	15	172	15	191	1.49e-78	243
Msa0263420	MtrunA17_Chr3g0124761	61.538	169	61	2	11	179	3	167	3.69e-72	224
Msa0263420	MtrunA17_Chr8g0384721	54.595	185	74	2	15	192	7	188	1.50e-70	220
Msa0263420	MtrunA17_Chr2g0318081	58.046	174	67	3	1	174	2	169	2.11e-67	214

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263420	AT1G69490.1	66.471	170	55	1	11	180	5	172	4.49e-81	247
Msa0263420	AT1G61110.1	67.647	170	38	3	15	172	16	180	3.34e-79	244
Msa0263420	AT3G15510.1	61.404	171	59	1	19	182	21	191	3.02e-76	238
Msa0263420	AT1G77450.1	58.989	178	69	2	9	186	4	177	1.52e-73	227
Msa0263420	AT3G04070.2	55.340	206	76	5	14	207	9	210	2.11e-73	230
Msa0263420	AT3G04070.1	53.153	222	72	6	14	207	9	226	1.32e-72	228
Msa0263420	AT1G01720.1	66.452	155	49	1	15	169	7	158	7.84e-72	224
Msa0263420	AT5G08790.1	58.857	175	68	2	10	183	2	173	1.01e-69	218
Msa0263420	AT1G52880.1	69.427	157	42	3	19	169	21	177	6.25e-69	218
Msa0263420	AT5G63790.1	57.865	178	70	3	10	185	45	219	6.18e-68	215

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Find 66 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCCGACATATTCTCAGT+TGG	0.595124	2_2:+62759480	None:intergenic
AACATACTTGGTAATTCCCA+TGG	0.531160	2_2:+62760208	None:intergenic
AACTCCTCCAACTAGAATAG+AGG	0.529579	2_2:+62759509	None:intergenic
AAGCCAATAATTTCTTCATG+TGG	0.547565	2_2:-62759966	Msa0263420:CDS
AATGGTATGTTTGGTTCACT+AGG	0.489315	2_2:+62759624	None:intergenic
ACATACCATTAAACCTACCA+AGG	0.620960	2_2:-62759611	Msa0263420:CDS
ACCTCCAACTGAGAATATGT+CGG	0.601698	2_2:-62759484	Msa0263420:CDS
ACGAAATCATTCAAACTCAA+AGG	0.572394	2_2:-62759834	Msa0263420:CDS
ACTATGAGTTCTTCATCAGA+AGG	0.609045	2_2:+62760310	None:intergenic
ACTTAAAAGAAAATCTAATG+AGG	0.595312	2_2:-62759349	Msa0263420:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAGTCAAGAACATACT+TGG	+	chr2_2:62759416-62759435	None:intergenic	30.0%
TTAATCCATTCGGATACTTT+CGG	+	chr2_2:62759573-62759592	None:intergenic	30.0%
AAGCCAATAATTTCTTCATG+TGG	-	chr2_2:62759643-62759662	Msa0263420:CDS	30.0%
GATCCACATGAAGAAATTAT+TGG	+	chr2_2:62759649-62759668	None:intergenic	30.0%
GATCATGAATGAGTATAGAT+TGG	-	chr2_2:62759741-62759760	Msa0263420:intron	30.0%
ACGAAATCATTCAAACTCAA+AGG	-	chr2_2:62759775-62759794	Msa0263420:intron	30.0%
GTTCAAGCACTATGAATAAA+GGG	+	chr2_2:62759855-62759874	None:intergenic	30.0%
AGTTCAAGCACTATGAATAA+AGG	+	chr2_2:62759856-62759875	None:intergenic	30.0%
ATTGAAAGTTCACGTTTGTA+GGG	+	chr2_2:62760035-62760054	None:intergenic	30.0%
AAAGAAGTCAAGAACATACT+TGG	+	chr2_2:62759416-62759435	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	62759233	62760398	62759233	ID=Msa0263420;Name=Msa0263420
chr2_2	mRNA	62759233	62760398	62759233	ID=Msa0263420-mRNA-1;Parent=Msa0263420;Name=Msa0263420-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0\|0\|1\|1\|1\|3\|0\|319
chr2_2	exon	62760215	62760398	62760215	ID=Msa0263420-mRNA-1:exon:19429;Parent=Msa0263420-mRNA-1
chr2_2	exon	62759824	62760110	62759824	ID=Msa0263420-mRNA-1:exon:19428;Parent=Msa0263420-mRNA-1
chr2_2	exon	62759233	62759721	62759233	ID=Msa0263420-mRNA-1:exon:19427;Parent=Msa0263420-mRNA-1
chr2_2	CDS	62760215	62760398	62760215	ID=Msa0263420-mRNA-1:cds;Parent=Msa0263420-mRNA-1
chr2_2	CDS	62759824	62760110	62759824	ID=Msa0263420-mRNA-1:cds;Parent=Msa0263420-mRNA-1
chr2_2	CDS	62759233	62759721	62759233	ID=Msa0263420-mRNA-1:cds;Parent=Msa0263420-mRNA-1

Gene Sequence

>Msa0263420

TTGATGGAGGGACAACAAAATGGATCCAAATCAAATTACACTTTTCCACCTGGTTTCAGATTCCATCCTTCTGATGAAGAACTCATAGTTCATTACTTGCAAAACAAAATCAAATCGCGTCCACTTCCAGCGTCGATTATAGCTGAAATTGATCTCTATAAGTATAATCCATGGGAATTACCAAAGAAGTCTTTGTTTGGTGAGGAAGAATGGTATTTCTTTAGTCCGAGAGACCGAAAGTATCCGAATGGATTAAGGCCAAATAGAACAGCAGCTTCAGGATACTGGAAGGCAACTGGAACTGATAAGCCAATAATTTCTTCATGTGGATCAAAGCATATAGGAGTGAAGAAATCTCTTGTGTTTTATTCAGGTAGACCTCCTAAGGGAGTTAAGACAGATTGGATCATGAATGAGTATAGATTGGTCGACACAACCACGAAATCATTCAAACTCAAAGGTTCCATGCGGCTGGATGACTGGGTATTATGTCGGGTTCGAAATAAAGGCTATTCATTGAAGAATTTATCTGAAAATCAAGAGAATCCTAGTGAACCAAACATACCATTAAACCTACCAAGGGGTGAAGAACGCCCTACAAACGTGAACTTTCAATCTGACATGATCAAAGATTATCAGTTCAAAGACTATCAGATCATAGCCTCTATTCTAGTTGGAGGAGTTATACCTCCAACTGAGAATATGTCGGGTTTGAGCGTTAATGGAAGCAAATGCAACAACATAACTTCAGTTTCTGAAGAAGGTTTCAATAAAGTGAATTCTCAAACTAAAATCCCTTCTTTAGAATGTTACTTCAATCCACTTAAAAGAAAATCTAATGAGGATGATGATCAGTTTGAACATCTCATTTCTTTTAACAGCAAGTTCAACACTGAGAACAAATTAGACAAATCTCCTAGCATCGATTTTCAAAAGCTAAACGAATTTACCTTTACATAA

Protein sequence

>Msa0263420

LMEGQQNGSKSNYTFPPGFRFHPSDEELIVHYLQNKIKSRPLPASIIAEIDLYKYNPWELPKKSLFGEEEWYFFSPRDRKYPNGLRPNRTAASGYWKATGTDKPIISSCGSKHIGVKKSLVFYSGRPPKGVKTDWIMNEYRLVDTTTKSFKLKGSMRLDDWVLCRVRNKGYSLKNLSENQENPSEPNIPLNLPRGEERPTNVNFQSDMIKDYQFKDYQIIASILVGGVIPPTENMSGLSVNGSKCNNITSVSEEGFNKVNSQTKIPSLECYFNPLKRKSNEDDDQFEHLISFNSKFNTENKLDKSPSIDFQKLNEFTFT*