AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0264440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0264440 MtrunA17_Chr2g0328321 98.387 124 2 0 1 124 1 124 2.63e-85 244
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0264440 AT5G59410.1 72.308 130 30 1 1 124 1 130 4.92e-65 193
Msa0264440 AT2G29020.1 70.940 117 34 0 8 124 17 133 4.09e-51 158

Find 84 sgRNAs with CRISPR-Local

Find 290 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCGGTGAAATTGGGAATT+AGG 0.168597 2_2:+64391823 None:intergenic
CACACGTTTGTGGCAAATTA+TGG 0.174659 2_2:-64388069 Msa0264440:three_prime_UTR
TTCCAGTGGTTGGAGGATTT+TGG 0.190036 2_2:-64390293 Msa0264440:CDS
CACTCTCTTTGTTTGTTACT+TGG 0.194473 2_2:-64391877 Msa0264440:five_prime_UTR
TTTATAATCAAAGTATTTAA+TGG 0.206914 2_2:+64387876 None:intergenic
CAAGCAATGGGCCTCGTATT+TGG 0.214066 2_2:-64390333 Msa0264440:CDS
CCTCGTATTTGGATTACTTT+GGG 0.235564 2_2:-64390322 Msa0264440:CDS
TCTAATCTGCGCAGCTTTCC+TGG 0.257466 2_2:-64388325 Msa0264440:intron
TGCTCGATCCCGAAGCTTCT+TGG 0.267462 2_2:-64391585 Msa0264440:CDS
TATTGACCCGTGTTTAATAA+AGG 0.296142 2_2:-64388014 Msa0264440:three_prime_UTR
TTTATATTTGATTTCATGTT+TGG 0.298071 2_2:-64388224 Msa0264440:three_prime_UTR
TTGTCCTCCGGGATCATATA+TGG 0.299115 2_2:-64388753 Msa0264440:CDS
TTATAAGATAAAATAAATAA+TGG 0.304898 2_2:+64391727 None:intergenic
AACGGCAAGAGCGGTGAAAT+TGG 0.307608 2_2:+64391815 None:intergenic
AGCTTCGGGATCGAGCAATT+TGG 0.310638 2_2:+64391591 None:intergenic
AGCGGTGAAATTGGGAATTA+GGG 0.318885 2_2:+64391824 None:intergenic
AAGGTTGATGAAGAGGAATT+TGG 0.323605 2_2:-64388711 Msa0264440:CDS
TGGATTCTAGTCTACAGTTT+AGG 0.323678 2_2:-64388305 Msa0264440:CDS
AAGGAGAAAGGTGACCATTC+TGG 0.360688 2_2:+64391623 None:intergenic
TGTTTAATAAAGGTGCTTGC+AGG 0.378128 2_2:-64388004 Msa0264440:three_prime_UTR
GCTCGATCCCGAAGCTTCTT+GGG 0.378769 2_2:-64391584 Msa0264440:CDS
GGAATTAGGGTTTGGTAAAA+CGG 0.379819 2_2:+64391837 None:intergenic
AACCTTGTCCCAAGAAGCTT+CGG 0.386161 2_2:+64391576 None:intergenic
GCCTCGTATTTGGATTACTT+TGG 0.386345 2_2:-64390323 Msa0264440:CDS
GCATCAGAGAGACAAAATAT+TGG 0.394751 2_2:+64387901 None:intergenic
TGAATTAATTAGTGTAAAGT+AGG 0.398454 2_2:+64391754 None:intergenic
GTGAAGAATTAGAAGATGAC+TGG 0.409526 2_2:+64391897 None:intergenic
AATTAAGTTCCAGTGAGTTA+TGG 0.410416 2_2:+64391921 None:intergenic
TTTGAATCATAAGGTAGATT+GGG 0.412860 2_2:+64391781 None:intergenic
TGAAATTGGGAATTAGGGTT+TGG 0.420838 2_2:+64391829 None:intergenic
TATAAGATAAAATAAATAAT+GGG 0.427970 2_2:+64391728 None:intergenic
TCATTGATTTCAGAACACTA+TGG 0.428967 2_2:+64388201 None:intergenic
CATCAGAGAGACAAAATATT+GGG 0.432128 2_2:+64387902 None:intergenic
TGGGGCGCCTTTCCAGTGGT+TGG 0.443020 2_2:-64390303 Msa0264440:CDS
TTAATGACACCACACGTTTG+TGG 0.447372 2_2:-64388079 Msa0264440:three_prime_UTR
ACGGCAAGAGCGGTGAAATT+GGG 0.458037 2_2:+64391816 None:intergenic
TGGCGAATTTGAAAGGAGAA+AGG 0.461188 2_2:+64391611 None:intergenic
GAAGAGGAATTTGGCGGCCA+TGG 0.466186 2_2:-64388702 Msa0264440:CDS
GAATTAATTAGTGTAAAGTA+GGG 0.468340 2_2:+64391755 None:intergenic
GAATTAGGGTTTGGTAAAAC+GGG 0.470212 2_2:+64391838 None:intergenic
CCATTCTGGTTGTGTTGTTG+AGG 0.471592 2_2:+64391637 None:intergenic
TTAGGACACTTCTGATCGGC+GGG 0.474638 2_2:-64388287 Msa0264440:exon
CCTCTTGGAGGAGGGCTCCA+TGG 0.480198 2_2:+64388685 None:intergenic
AGCAATTTGGCGAATTTGAA+AGG 0.482080 2_2:+64391604 None:intergenic
ATGAGAAAATGCGGGTTGGC+GGG 0.484750 2_2:-64388182 Msa0264440:three_prime_UTR
TTACTATTCAGTAATATTGA+AGG 0.488058 2_2:-64388730 Msa0264440:CDS
CATTGGATTCGACAAGCAAT+GGG 0.488187 2_2:-64390345 Msa0264440:CDS
AACTTGGAGATGTATTGCAT+TGG 0.493240 2_2:-64390362 Msa0264440:CDS
GCATTGGATTCGACAAGCAA+TGG 0.498506 2_2:-64390346 Msa0264440:CDS
ACCTTGTCCCAAGAAGCTTC+GGG 0.501872 2_2:+64391577 None:intergenic
CATGGAGCCCTCCTCCAAGA+GGG 0.507327 2_2:-64388684 Msa0264440:CDS
TAAACTGTAGACTAGAATCC+AGG 0.517183 2_2:+64388307 None:intergenic
AACCAAAATCCTCCAACCAC+TGG 0.518282 2_2:+64390291 None:intergenic
GGCGCCTTTCCAGTGGTTGG+AGG 0.519426 2_2:-64390300 Msa0264440:CDS
AATGAGAAAATGCGGGTTGG+CGG 0.525036 2_2:-64388183 Msa0264440:three_prime_UTR
AATATTGAAGGTTGATGAAG+AGG 0.533923 2_2:-64388718 Msa0264440:CDS
ATGGAGCCCTCCTCCAAGAG+GGG 0.538216 2_2:-64388683 Msa0264440:CDS
TGAAGAATTAGAAGATGACT+GGG 0.540663 2_2:+64391898 None:intergenic
GATTGGGTAACTACGAGAAA+CGG 0.544095 2_2:+64391797 None:intergenic
TCCCGAAGCTTCTTGGGACA+AGG 0.549633 2_2:-64391578 Msa0264440:intron
GTTGATGAAGAGGAATTTGG+CGG 0.560236 2_2:-64388708 Msa0264440:CDS
AACCCCAAATAGGATCAACT+TGG 0.560316 2_2:-64390378 Msa0264440:intron
CTCGTATTTGGATTACTTTG+GGG 0.563337 2_2:-64390321 Msa0264440:CDS
CTGAAATCAATGAGAAAATG+CGG 0.568466 2_2:-64388191 Msa0264440:three_prime_UTR
TGAAATCAATGAGAAAATGC+GGG 0.575073 2_2:-64388190 Msa0264440:three_prime_UTR
CCTCAACAACACAACCAGAA+TGG 0.579984 2_2:-64391637 Msa0264440:CDS
AAATCCTCCAACCACTGGAA+AGG 0.583783 2_2:+64390296 None:intergenic
ATAATCAAAGTATTTAATGG+AGG 0.593603 2_2:+64387879 None:intergenic
CCATGGAGCCCTCCTCCAAG+AGG 0.596639 2_2:-64388685 Msa0264440:CDS
TTAATATCATAATTCAAGTG+TGG 0.597042 2_2:+64387847 None:intergenic
ACTTTGGGGCGCCTTTCCAG+TGG 0.601275 2_2:-64390307 Msa0264440:CDS
TGCTTGCAGGCAAATTCTGA+AGG 0.601769 2_2:-64387991 Msa0264440:three_prime_UTR
GCAAGCACCTTTATTAAACA+CGG 0.602743 2_2:+64388007 None:intergenic
ATCAATGAGAAAATGCGGGT+TGG 0.603593 2_2:-64388186 Msa0264440:three_prime_UTR
ACTACGAGAAACGGCAAGAG+CGG 0.607619 2_2:+64391806 None:intergenic
GATATCTTCAGAATTTGCAA+AGG 0.610174 2_2:+64387965 None:intergenic
ATAGTAACCATATATGATCC+CGG 0.618972 2_2:+64388746 None:intergenic
CATAATTTGCCACAAACGTG+TGG 0.620918 2_2:+64388070 None:intergenic
CAGTTTAGGACACTTCTGAT+CGG 0.623936 2_2:-64388291 Msa0264440:exon
TTTAGGACACTTCTGATCGG+CGG 0.628007 2_2:-64388288 Msa0264440:exon
CCCAAAGTAATCCAAATACG+AGG 0.634018 2_2:+64390322 None:intergenic
CAAGCACCTTTATTAAACAC+GGG 0.647466 2_2:+64388008 None:intergenic
TAGGACACTTCTGATCGGCG+GGG 0.755597 2_2:-64388286 Msa0264440:exon
GTAACCATATATGATCCCGG+AGG 0.792581 2_2:+64388749 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTATATAAACTATGT+GGG + chr2_2:64389166-64389185 None:intergenic 10.0%
!!! TTTTTTTTATATAAACTATG+TGG + chr2_2:64389167-64389186 None:intergenic 10.0%
!!! TTTATAATCAAAGTATTTAA+TGG + chr2_2:64391891-64391910 None:intergenic 10.0%
!!! TTATTTTTGAGTAAAAAAAG+TGG - chr2_2:64388307-64388326 Msa0264440:CDS 15.0%
!!! TATTTTTGAGTAAAAAAAGT+GGG - chr2_2:64388308-64388327 Msa0264440:CDS 15.0%
!!! TGGGTTTTATTAATATATTA+TGG - chr2_2:64388327-64388346 Msa0264440:intron 15.0%
!! TAATTGATTATATTTAGACA+CGG - chr2_2:64388472-64388491 Msa0264440:intron 15.0%
!! AATAAAATTTACACATATAG+GGG + chr2_2:64389847-64389866 None:intergenic 15.0%
!! GAATAAAATTTACACATATA+GGG + chr2_2:64389848-64389867 None:intergenic 15.0%
!! TGAATAAAATTTACACATAT+AGG + chr2_2:64389849-64389868 None:intergenic 15.0%
!! TGATATATGATAAAACATTA+TGG - chr2_2:64390038-64390057 Msa0264440:intron 15.0%
!!! TACTATTTGTTTTCATTTTT+TGG - chr2_2:64390130-64390149 Msa0264440:intron 15.0%
!!! ATAAATTTTCTAATACATAC+TGG + chr2_2:64390235-64390254 None:intergenic 15.0%
!! ATTACCTAAAATTAAGTTAA+GGG + chr2_2:64390527-64390546 None:intergenic 15.0%
!!! TTTATATTTGATTTCATGTT+TGG - chr2_2:64391540-64391559 Msa0264440:intron 15.0%
!! GAATTAATTAGTGTAAAGTA+GGG + chr2_2:64388012-64388031 None:intergenic 20.0%
!! TGAATTAATTAGTGTAAAGT+AGG + chr2_2:64388013-64388032 None:intergenic 20.0%
!! CTCAAAAATAAACAAAATCT+GGG + chr2_2:64388298-64388317 None:intergenic 20.0%
!! ACTCAAAAATAAACAAAATC+TGG + chr2_2:64388299-64388318 None:intergenic 20.0%
!! AAAACTTGAGAGTAAATTAA+TGG + chr2_2:64388369-64388388 None:intergenic 20.0%
!! ATTACTCATCTAATTAAACA+TGG + chr2_2:64388437-64388456 None:intergenic 20.0%
!! AATGAATCATGAACAAATTA+AGG + chr2_2:64388591-64388610 None:intergenic 20.0%
!! TACGGAAAAATATATTTGTT+GGG + chr2_2:64388762-64388781 None:intergenic 20.0%
!! AAAACTAAATGGTTAATCTA+GGG + chr2_2:64389076-64389095 None:intergenic 20.0%
!!! GTATTGACATTAAATTATGT+AGG + chr2_2:64389670-64389689 None:intergenic 20.0%
!! ATAAAATTTACACATATAGG+GGG + chr2_2:64389846-64389865 None:intergenic 20.0%
!!! TGTAAATTTTATTCAGAACT+TGG - chr2_2:64389854-64389873 Msa0264440:intron 20.0%
!! TTACCTAAAATTAAGTTAAG+GGG + chr2_2:64390526-64390545 None:intergenic 20.0%
!! CATTACCTAAAATTAAGTTA+AGG + chr2_2:64390528-64390547 None:intergenic 20.0%
!!! TTTTTTTCTTCACTTCATTT+TGG - chr2_2:64390567-64390586 Msa0264440:intron 20.0%
!!! TTTTTTTTTCCACATCATTT+TGG + chr2_2:64390742-64390761 None:intergenic 20.0%
!!! AATGTGTACTATTTTAAACT+AGG + chr2_2:64390817-64390836 None:intergenic 20.0%
!!! AGTACACATTATGTTTTTAT+TGG - chr2_2:64390827-64390846 Msa0264440:intron 20.0%
!! TTACTATTCAGTAATATTGA+AGG - chr2_2:64391034-64391053 Msa0264440:intron 20.0%
!!! GCAAATTTAATTTTTTGTGT+GGG + chr2_2:64391179-64391198 None:intergenic 20.0%
!!! AGCAAATTTAATTTTTTGTG+TGG + chr2_2:64391180-64391199 None:intergenic 20.0%
!!! GCAAATTTAATTTTTTGTGT+GGG + chr2_2:64391179-64391198 None:intergenic 20.0%
!!! AGCAAATTTAATTTTTTGTG+TGG + chr2_2:64391180-64391199 None:intergenic 20.0%
!!! GCAAATTTAATTTTTTGTGT+GGG + chr2_2:64391179-64391198 None:intergenic 20.0%
!!! AGCAAATTTAATTTTTTGTG+TGG + chr2_2:64391180-64391199 None:intergenic 20.0%
!!! ATAATCAAAGTATTTAATGG+AGG + chr2_2:64391888-64391907 None:intergenic 20.0%
!! TTAATATCATAATTCAAGTG+TGG + chr2_2:64391920-64391939 None:intergenic 20.0%
!!! TTTTATTTTCCATAACTCAC+TGG - chr2_2:64387834-64387853 Msa0264440:three_prime_UTR 25.0%
! TTTGAATCATAAGGTAGATT+GGG + chr2_2:64387986-64388005 None:intergenic 25.0%
!! TTTTGAATCATAAGGTAGAT+TGG + chr2_2:64387987-64388006 None:intergenic 25.0%
!!! GACTTTTTTTTCTTTAGAGT+TGG - chr2_2:64388521-64388540 Msa0264440:intron 25.0%
! TCATGATTCATTGATATTGT+TGG - chr2_2:64388599-64388618 Msa0264440:intron 25.0%
! GTACGGAAAAATATATTTGT+TGG + chr2_2:64388763-64388782 None:intergenic 25.0%
! AAACTACACTATAATCAAAC+GGG + chr2_2:64388958-64388977 None:intergenic 25.0%
! GAAACTACACTATAATCAAA+CGG + chr2_2:64388959-64388978 None:intergenic 25.0%
!! TATAGTGTAGTTTCTTCTTT+TGG - chr2_2:64388965-64388984 Msa0264440:intron 25.0%
!! ATAGTGTAGTTTCTTCTTTT+GGG - chr2_2:64388966-64388985 Msa0264440:intron 25.0%
! GAAAACTAAATGGTTAATCT+AGG + chr2_2:64389077-64389096 None:intergenic 25.0%
! ACAAATCAGTGAAAACTAAA+TGG + chr2_2:64389087-64389106 None:intergenic 25.0%
!!! TTAGTTTTCACTGATTTGTT+TGG - chr2_2:64389088-64389107 Msa0264440:intron 25.0%
! TTAAGATAAACATACAGGAT+CGG + chr2_2:64389521-64389540 None:intergenic 25.0%
!!! CAACTTTAAGATAAACATAC+AGG + chr2_2:64389526-64389545 None:intergenic 25.0%
!!! AAGATGTGCTATTTTTTAAG+TGG + chr2_2:64389907-64389926 None:intergenic 25.0%
! TGTCAACTAATGAGTTATTT+AGG - chr2_2:64390011-64390030 Msa0264440:intron 25.0%
! GTCAACTAATGAGTTATTTA+GGG - chr2_2:64390012-64390031 Msa0264440:intron 25.0%
! AAAATGTGACTTAAGTTACA+TGG - chr2_2:64390169-64390188 Msa0264440:intron 25.0%
! TGTATACAGTATATCTAGAA+AGG + chr2_2:64390270-64390289 None:intergenic 25.0%
! AATAACTAAACAAGCATCTA+AGG + chr2_2:64390405-64390424 None:intergenic 25.0%
!!! AGTACTGGTTATATTTTGAT+TGG + chr2_2:64390430-64390449 None:intergenic 25.0%
! TTGAAATACATATCCAAACA+TGG + chr2_2:64390602-64390621 None:intergenic 25.0%
!!! AAATCTAAGGTTTTCTTTTG+GGG + chr2_2:64390682-64390701 None:intergenic 25.0%
!!! CAAATCTAAGGTTTTCTTTT+GGG + chr2_2:64390683-64390702 None:intergenic 25.0%
!!! TCAAATCTAAGGTTTTCTTT+TGG + chr2_2:64390684-64390703 None:intergenic 25.0%
AATTAAGTTCCAGTGAGTTA+TGG + chr2_2:64387846-64387865 None:intergenic 30.0%
TGAAGAATTAGAAGATGACT+GGG + chr2_2:64387869-64387888 None:intergenic 30.0%
! GTAGGGATTTTTGAATCATA+AGG + chr2_2:64387995-64388014 None:intergenic 30.0%
AGATCGAAAAACATGAAAAC+AGG + chr2_2:64388233-64388252 None:intergenic 30.0%
!!! AGGTTGTTTTTTCAGGAAAT+AGG - chr2_2:64388258-64388277 Msa0264440:three_prime_UTR 30.0%
TAAATGCAACCAACATAGTT+TGG + chr2_2:64388835-64388854 None:intergenic 30.0%
GATCATAGATAATGAGATCA+TGG + chr2_2:64388874-64388893 None:intergenic 30.0%
CAAAACTGAATGCTTAATCA+AGG + chr2_2:64388906-64388925 None:intergenic 30.0%
!!! ATTCAGTTTTGCTGATTTGT+TGG - chr2_2:64388915-64388934 Msa0264440:intron 30.0%
!!! TTCAGTTTTGCTGATTTGTT+GGG - chr2_2:64388916-64388935 Msa0264440:intron 30.0%
AACTACACTATAATCAAACG+GGG + chr2_2:64388957-64388976 None:intergenic 30.0%
CATGTAGTATGTGATTGATA+TGG - chr2_2:64389213-64389232 Msa0264440:intron 30.0%
ATTGTGGAAGATACTTTAGA+AGG - chr2_2:64389251-64389270 Msa0264440:intron 30.0%
AAAGATAAAAACAGAGAAGC+TGG + chr2_2:64389357-64389376 None:intergenic 30.0%
TCGGTAAAATAAACACTTAC+AGG + chr2_2:64389502-64389521 None:intergenic 30.0%
ATCAGTGAATATGAGAATTC+TGG + chr2_2:64389600-64389619 None:intergenic 30.0%
TCGTGCATAAACAACTTTAT+TGG - chr2_2:64389629-64389648 Msa0264440:intron 30.0%
CGTGCATAAACAACTTTATT+GGG - chr2_2:64389630-64389649 Msa0264440:intron 30.0%
! ATTATCTGAAATACCAATGC+TGG - chr2_2:64389709-64389728 Msa0264440:intron 30.0%
! AACAAAATTTTCTCCAGCAT+TGG + chr2_2:64389725-64389744 None:intergenic 30.0%
! AATGCTGGAGAAAATTTTGT+TGG - chr2_2:64389724-64389743 Msa0264440:intron 30.0%
ATGACAACTTGTAAGAACAA+AGG + chr2_2:64389753-64389772 None:intergenic 30.0%
CTAAGTGTGATGCAAATATT+GGG + chr2_2:64389776-64389795 None:intergenic 30.0%
! TGCTCTAAGTACAGAAATTA+GGG - chr2_2:64389818-64389837 Msa0264440:intron 30.0%
GGTCATGAAATCAAAAACAA+TGG - chr2_2:64389875-64389894 Msa0264440:intron 30.0%
CATGAAATCAAAAACAATGG+AGG - chr2_2:64389878-64389897 Msa0264440:intron 30.0%
GATATAAAAACGAAGAACTG+AGG + chr2_2:64389934-64389953 None:intergenic 30.0%
! TGATCGTCTTTTGCATAAAT+TGG - chr2_2:64390198-64390217 Msa0264440:intron 30.0%
!!! GTGAAATTTTTTGTCCAGAA+GGG - chr2_2:64390321-64390340 Msa0264440:CDS 30.0%
TCTCAGTCTTAAGTATATGT+TGG - chr2_2:64390655-64390674 Msa0264440:intron 30.0%
CTCAGTCTTAAGTATATGTT+GGG - chr2_2:64390656-64390675 Msa0264440:intron 30.0%
ATTCTCAAGCTTCAAATCTA+AGG + chr2_2:64390695-64390714 None:intergenic 30.0%
TGAGAATCCATATACAAACA+TGG - chr2_2:64390708-64390727 Msa0264440:intron 30.0%
! CTTAATTTCAGGTAACGATT+TGG + chr2_2:64390770-64390789 None:intergenic 30.0%
TCGTTACCTGAAATTAAGTT+TGG - chr2_2:64390772-64390791 Msa0264440:intron 30.0%
CGTTACCTGAAATTAAGTTT+GGG - chr2_2:64390773-64390792 Msa0264440:intron 30.0%
! AAACTTTTCATTGCACTAGT+AGG - chr2_2:64390903-64390922 Msa0264440:intron 30.0%
AAAAAGCTGCAATACAAACA+GGG + chr2_2:64390987-64391006 None:intergenic 30.0%
TAAAAAGCTGCAATACAAAC+AGG + chr2_2:64390988-64391007 None:intergenic 30.0%
ATAGTAACCATATATGATCC+CGG + chr2_2:64391021-64391040 None:intergenic 30.0%
!! AATATTGAAGGTTGATGAAG+AGG - chr2_2:64391046-64391065 Msa0264440:intron 30.0%
AGAAAAGAAGTCTTAGATTG+TGG + chr2_2:64391216-64391235 None:intergenic 30.0%
AAACTTTCTACTGTTAATCC+AGG + chr2_2:64391254-64391273 None:intergenic 30.0%
AGAAAAGAAGTCTTAGATTG+TGG + chr2_2:64391216-64391235 None:intergenic 30.0%
AAACTTTCTACTGTTAATCC+AGG + chr2_2:64391254-64391273 None:intergenic 30.0%
AGAAAAGAAGTCTTAGATTG+TGG + chr2_2:64391216-64391235 None:intergenic 30.0%
!! TCATTGATTTCAGAACACTA+TGG + chr2_2:64391566-64391585 None:intergenic 30.0%
CTGAAATCAATGAGAAAATG+CGG - chr2_2:64391573-64391592 Msa0264440:intron 30.0%
TGAAATCAATGAGAAAATGC+GGG - chr2_2:64391574-64391593 Msa0264440:intron 30.0%
! AAAACATTCTGATTTTGTCG+AGG - chr2_2:64391622-64391641 Msa0264440:CDS 30.0%
! AAACATTCTGATTTTGTCGA+GGG - chr2_2:64391623-64391642 Msa0264440:CDS 30.0%
TATTGACCCGTGTTTAATAA+AGG - chr2_2:64391750-64391769 Msa0264440:five_prime_UTR 30.0%
GATATCTTCAGAATTTGCAA+AGG + chr2_2:64391802-64391821 None:intergenic 30.0%
CATCAGAGAGACAAAATATT+GGG + chr2_2:64391865-64391884 None:intergenic 30.0%
!!! ATTAGTTTTGCACTATGTTG+AGG - chr2_2:64391955-64391974 Msa0264440:five_prime_UTR 30.0%
!!! TTAGTTTTGCACTATGTTGA+GGG - chr2_2:64391956-64391975 Msa0264440:five_prime_UTR 30.0%
GTGAAGAATTAGAAGATGAC+TGG + chr2_2:64387870-64387889 None:intergenic 35.0%
! CACTCTCTTTGTTTGTTACT+TGG - chr2_2:64387887-64387906 Msa0264440:three_prime_UTR 35.0%
! GAATTAGGGTTTGGTAAAAC+GGG + chr2_2:64387929-64387948 None:intergenic 35.0%
! GGAATTAGGGTTTGGTAAAA+CGG + chr2_2:64387930-64387949 None:intergenic 35.0%
TGAAATTGGGAATTAGGGTT+TGG + chr2_2:64387938-64387957 None:intergenic 35.0%
AGCAATTTGGCGAATTTGAA+AGG + chr2_2:64388163-64388182 None:intergenic 35.0%
!! CATGTTTTTCGATCTGTTTG+AGG - chr2_2:64388238-64388257 Msa0264440:three_prime_UTR 35.0%
!!! CTGTTTGAGGTTGTTTTTTC+AGG - chr2_2:64388251-64388270 Msa0264440:three_prime_UTR 35.0%
CGGTATAAGTATCAGTGATT+TGG - chr2_2:64388492-64388511 Msa0264440:intron 35.0%
AGAGAGTGAGATCCATTTAA+GGG - chr2_2:64388726-64388745 Msa0264440:CDS 35.0%
! ATATATTTTTCCGTACGCAC+CGG - chr2_2:64388767-64388786 Msa0264440:intron 35.0%
ACTTGGGTCTCTTAAGTATT+CGG + chr2_2:64388798-64388817 None:intergenic 35.0%
TGCATTTAGCTGATATGAGT+TGG - chr2_2:64388847-64388866 Msa0264440:intron 35.0%
!!! TTTTGCTGATTTGTTGGGTT+CGG - chr2_2:64388921-64388940 Msa0264440:intron 35.0%
!! ATTTAACAGTGGTTCTGAGT+TGG - chr2_2:64389018-64389037 Msa0264440:intron 35.0%
ACTAAATGGTTAATCTAGGG+TGG + chr2_2:64389073-64389092 None:intergenic 35.0%
GATGTAGCTTCTCTAAATTG+TGG - chr2_2:64389235-64389254 Msa0264440:intron 35.0%
! TTTTGCACTTCTCATTCTAG+AGG - chr2_2:64389297-64389316 Msa0264440:intron 35.0%
TAAAAACAGAGAAGCTGGTT+AGG + chr2_2:64389352-64389371 None:intergenic 35.0%
!! TCTCCAAGTTGATCCTATTT+GGG + chr2_2:64389392-64389411 None:intergenic 35.0%
!! ATCTCCAAGTTGATCCTATT+TGG + chr2_2:64389393-64389412 None:intergenic 35.0%
AACTTGGAGATGTATTGCAT+TGG - chr2_2:64389402-64389421 Msa0264440:intron 35.0%
CCTCGTATTTGGATTACTTT+GGG - chr2_2:64389442-64389461 Msa0264440:intron 35.0%
CTCGTATTTGGATTACTTTG+GGG - chr2_2:64389443-64389462 Msa0264440:intron 35.0%
TCTGGAAAGACTAAATCATC+AGG + chr2_2:64389582-64389601 None:intergenic 35.0%
CCTAAGTGTGATGCAAATAT+TGG + chr2_2:64389777-64389796 None:intergenic 35.0%
CCAATATTTGCATCACACTT+AGG - chr2_2:64389774-64389793 Msa0264440:intron 35.0%
! CTGCTCTAAGTACAGAAATT+AGG - chr2_2:64389817-64389836 Msa0264440:intron 35.0%
CCTTGCAAAGCAAAATTTGA+TGG + chr2_2:64390302-64390321 None:intergenic 35.0%
! CCATCAAATTTTGCTTTGCA+AGG - chr2_2:64390299-64390318 Msa0264440:CDS 35.0%
!!! GGTGAAATTTTTTGTCCAGA+AGG - chr2_2:64390320-64390339 Msa0264440:CDS 35.0%
AAAGACAAATGATGAAGGTG+AGG + chr2_2:64390350-64390369 None:intergenic 35.0%
TTGGGAAAGACAAATGATGA+AGG + chr2_2:64390355-64390374 None:intergenic 35.0%
!! TAGAGTTTTGTCGTAAGTCT+GGG + chr2_2:64390378-64390397 None:intergenic 35.0%
!! ATAGAGTTTTGTCGTAAGTC+TGG + chr2_2:64390379-64390398 None:intergenic 35.0%
TGAGGTGTAAAATACAGTAC+TGG + chr2_2:64390445-64390464 None:intergenic 35.0%
ATGTGATGATCTCACATTTG+AGG + chr2_2:64390463-64390482 None:intergenic 35.0%
ACAAGTGTGAGGAAGTTAAA+AGG - chr2_2:64390483-64390502 Msa0264440:intron 35.0%
! CTGCCCCTTAACTTAATTTT+AGG - chr2_2:64390520-64390539 Msa0264440:intron 35.0%
CATATCCAAACATGGATAGA+AGG + chr2_2:64390594-64390613 None:intergenic 35.0%
CCTTCATCCATGTTTGTATA+TGG + chr2_2:64390718-64390737 None:intergenic 35.0%
CCATATACAAACATGGATGA+AGG - chr2_2:64390715-64390734 Msa0264440:intron 35.0%
! TGAAATTAAGTTTGGGACCA+CGG - chr2_2:64390780-64390799 Msa0264440:intron 35.0%
AAACAGGGAAACTCTACTTT+AGG + chr2_2:64390972-64390991 None:intergenic 35.0%
!!! AGCTTTTTAGTTTTGTCCTC+CGG - chr2_2:64390999-64391018 Msa0264440:intron 35.0%
!! AAGGTTGATGAAGAGGAATT+TGG - chr2_2:64391053-64391072 Msa0264440:intron 35.0%
! TGCATCTACAACTCTTTTTC+TGG - chr2_2:64391109-64391128 Msa0264440:intron 35.0%
TAAACTGTAGACTAGAATCC+AGG + chr2_2:64391460-64391479 None:intergenic 35.0%
TGGATTCTAGTCTACAGTTT+AGG - chr2_2:64391459-64391478 Msa0264440:intron 35.0%
CAAGCACCTTTATTAAACAC+GGG + chr2_2:64391759-64391778 None:intergenic 35.0%
GCAAGCACCTTTATTAAACA+CGG + chr2_2:64391760-64391779 None:intergenic 35.0%
!! TGTTTAATAAAGGTGCTTGC+AGG - chr2_2:64391760-64391779 Msa0264440:five_prime_UTR 35.0%
GCATCAGAGAGACAAAATAT+TGG + chr2_2:64391866-64391885 None:intergenic 35.0%
AGCGGTGAAATTGGGAATTA+GGG + chr2_2:64387943-64387962 None:intergenic 40.0%
GATTGGGTAACTACGAGAAA+CGG + chr2_2:64387970-64387989 None:intergenic 40.0%
TGGCGAATTTGAAAGGAGAA+AGG + chr2_2:64388156-64388175 None:intergenic 40.0%
ATTGTGAGCAGAGGATAACT+TGG + chr2_2:64388699-64388718 None:intergenic 40.0%
CTCAGATAAATTGTGAGCAG+AGG + chr2_2:64388708-64388727 None:intergenic 40.0%
GAGAGAGTGAGATCCATTTA+AGG - chr2_2:64388725-64388744 Msa0264440:CDS 40.0%
GAGAGTGAGATCCATTTAAG+GGG - chr2_2:64388727-64388746 Msa0264440:CDS 40.0%
GAATGCTTAATCAAGGACAG+AGG + chr2_2:64388899-64388918 None:intergenic 40.0%
!! TTTTGGGCTTTAATGCGACA+TGG - chr2_2:64388982-64389001 Msa0264440:intron 40.0%
GCTGGTGGTTTATTTAACAG+TGG - chr2_2:64389007-64389026 Msa0264440:intron 40.0%
ACTGCAATCAAACAAGGAGT+AGG + chr2_2:64389118-64389137 None:intergenic 40.0%
AACTGCACTGCAATCAAACA+AGG + chr2_2:64389124-64389143 None:intergenic 40.0%
! GCAGTGCAGTTTCTTCTTTT+TGG - chr2_2:64389133-64389152 Msa0264440:intron 40.0%
CAGCTAAGCATAGCAATTAC+TGG + chr2_2:64389193-64389212 None:intergenic 40.0%
! AGTTGATCCTATTTGGGGTT+GGG + chr2_2:64389386-64389405 None:intergenic 40.0%
! AAGTTGATCCTATTTGGGGT+TGG + chr2_2:64389387-64389406 None:intergenic 40.0%
AACCCCAAATAGGATCAACT+TGG - chr2_2:64389386-64389405 Msa0264440:intron 40.0%
!! CTCCAAGTTGATCCTATTTG+GGG + chr2_2:64389391-64389410 None:intergenic 40.0%
CATTGGATTCGACAAGCAAT+GGG - chr2_2:64389419-64389438 Msa0264440:intron 40.0%
GCCTCGTATTTGGATTACTT+TGG - chr2_2:64389441-64389460 Msa0264440:intron 40.0%
CCCAAAGTAATCCAAATACG+AGG + chr2_2:64389445-64389464 None:intergenic 40.0%
! TTTTGTCGTAAGTCTGGGTT+GGG + chr2_2:64390373-64390392 None:intergenic 40.0%
GATCATCACATACAAGTGTG+AGG - chr2_2:64390472-64390491 Msa0264440:intron 40.0%
AAATTAAGTTAAGGGGCAGC+AGG + chr2_2:64390519-64390538 None:intergenic 40.0%
TTGGTCCTTCTATCCATGTT+TGG - chr2_2:64390586-64390605 Msa0264440:intron 40.0%
GATGAAGGACCAAAATGATG+TGG - chr2_2:64390730-64390749 Msa0264440:intron 40.0%
GTGGTCCCAAACTTAATTTC+AGG + chr2_2:64390781-64390800 None:intergenic 40.0%
ACTAGGCAAAATTACTTCCG+TGG + chr2_2:64390800-64390819 None:intergenic 40.0%
ACCTGGGAATTGTATGAGTT+TGG - chr2_2:64390875-64390894 Msa0264440:intron 40.0%
ACCAAACTCATACAATTCCC+AGG + chr2_2:64390879-64390898 None:intergenic 40.0%
!!! GCTTTTTAGTTTTGTCCTCC+GGG - chr2_2:64391000-64391019 Msa0264440:intron 40.0%
! GTTGATGAAGAGGAATTTGG+CGG - chr2_2:64391056-64391075 Msa0264440:intron 40.0%
! CAGTTTAGGACACTTCTGAT+CGG - chr2_2:64391473-64391492 Msa0264440:intron 40.0%
ATCAATGAGAAAATGCGGGT+TGG - chr2_2:64391578-64391597 Msa0264440:intron 40.0%
TTAATGACACCACACGTTTG+TGG - chr2_2:64391685-64391704 Msa0264440:five_prime_UTR 40.0%
! CATAATTTGCCACAAACGTG+TGG + chr2_2:64391697-64391716 None:intergenic 40.0%
CACACGTTTGTGGCAAATTA+TGG - chr2_2:64391695-64391714 Msa0264440:five_prime_UTR 40.0%
GAGCGGTGAAATTGGGAATT+AGG + chr2_2:64387944-64387963 None:intergenic 45.0%
! CCATTCTGGTTGTGTTGTTG+AGG + chr2_2:64388130-64388149 None:intergenic 45.0%
CCTCAACAACACAACCAGAA+TGG - chr2_2:64388127-64388146 Msa0264440:three_prime_UTR 45.0%
AAGGAGAAAGGTGACCATTC+TGG + chr2_2:64388144-64388163 None:intergenic 45.0%
AACCTTGTCCCAAGAAGCTT+CGG + chr2_2:64388191-64388210 None:intergenic 45.0%
GGAGAAGTCAACCCCTTAAA+TGG + chr2_2:64388741-64388760 None:intergenic 45.0%
TCTTAAGTATTCGGTCTGGC+CGG + chr2_2:64388789-64388808 None:intergenic 45.0%
GGTCTCTTAAGTATTCGGTC+TGG + chr2_2:64388793-64388812 None:intergenic 45.0%
GGCACAAGTGCTAGATACTT+GGG + chr2_2:64388814-64388833 None:intergenic 45.0%
TGGCACAAGTGCTAGATACT+TGG + chr2_2:64388815-64388834 None:intergenic 45.0%
GCACTTGTGCCAAACTATGT+TGG - chr2_2:64388823-64388842 Msa0264440:intron 45.0%
CTTTAATGCGACATGGTTGC+TGG - chr2_2:64388989-64389008 Msa0264440:intron 45.0%
! GTTGATCCTATTTGGGGTTG+GGG + chr2_2:64389385-64389404 None:intergenic 45.0%
GCATTGGATTCGACAAGCAA+TGG - chr2_2:64389418-64389437 Msa0264440:intron 45.0%
AAATCCTCCAACCACTGGAA+AGG + chr2_2:64389471-64389490 None:intergenic 45.0%
! TTCCAGTGGTTGGAGGATTT+TGG - chr2_2:64389471-64389490 Msa0264440:intron 45.0%
AACCAAAATCCTCCAACCAC+TGG + chr2_2:64389476-64389495 None:intergenic 45.0%
TCACTCTATCCATAAGCCAC+TGG - chr2_2:64389552-64389571 Msa0264440:intron 45.0%
TAAATCATCAGGCAAGCCAG+TGG + chr2_2:64389571-64389590 None:intergenic 45.0%
! AAGCCTTATCCCACTAAGTG+GGG + chr2_2:64390087-64390106 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG + chr2_2:64390088-64390107 None:intergenic 45.0%
! GTTTTGTCGTAAGTCTGGGT+TGG + chr2_2:64390374-64390393 None:intergenic 45.0%
CTTAAGACTGAGAGCTCACT+GGG + chr2_2:64390648-64390667 None:intergenic 45.0%
ACTTAAGACTGAGAGCTCAC+TGG + chr2_2:64390649-64390668 None:intergenic 45.0%
ATACAATTCCCAGGTGCTTG+TGG + chr2_2:64390870-64390889 None:intergenic 45.0%
TTGTCCTCCGGGATCATATA+TGG - chr2_2:64391011-64391030 Msa0264440:intron 45.0%
GTAACCATATATGATCCCGG+AGG + chr2_2:64391018-64391037 None:intergenic 45.0%
TAGATGCAAAAAGCCCCTCT+TGG + chr2_2:64391097-64391116 None:intergenic 45.0%
! TTTAGGACACTTCTGATCGG+CGG - chr2_2:64391476-64391495 Msa0264440:intron 45.0%
!! AATGAGAAAATGCGGGTTGG+CGG - chr2_2:64391581-64391600 Msa0264440:CDS 45.0%
! CATTCTGATTTTGTCGAGGG+TGG - chr2_2:64391626-64391645 Msa0264440:CDS 45.0%
TGCTTGCAGGCAAATTCTGA+AGG - chr2_2:64391773-64391792 Msa0264440:five_prime_UTR 45.0%
!! TATAAGATAAAATAAATAAT+GGG + chr2_2:64388039-64388058 None:intergenic 5.0%
!! TTATAAGATAAAATAAATAA+TGG + chr2_2:64388040-64388059 None:intergenic 5.0%
ACGGCAAGAGCGGTGAAATT+GGG + chr2_2:64387951-64387970 None:intergenic 50.0%
AACGGCAAGAGCGGTGAAAT+TGG + chr2_2:64387952-64387971 None:intergenic 50.0%
ACTACGAGAAACGGCAAGAG+CGG + chr2_2:64387961-64387980 None:intergenic 50.0%
AGCTTCGGGATCGAGCAATT+TGG + chr2_2:64388176-64388195 None:intergenic 50.0%
ACCTTGTCCCAAGAAGCTTC+GGG + chr2_2:64388190-64388209 None:intergenic 50.0%
TAATGCGACATGGTTGCTGG+TGG - chr2_2:64388992-64389011 Msa0264440:intron 50.0%
CAAGCAATGGGCCTCGTATT+TGG - chr2_2:64389431-64389450 Msa0264440:intron 50.0%
CTTATCCCACTAAGTGGGGT+CGG + chr2_2:64390083-64390102 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG + chr2_2:64390089-64390108 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG - chr2_2:64390086-64390105 Msa0264440:intron 50.0%
TGAAGGTGAGGATTCCCTTC+TGG + chr2_2:64390338-64390357 None:intergenic 50.0%
ACCACAAATCCACAAGCACC+TGG - chr2_2:64390858-64390877 Msa0264440:intron 50.0%
CCACAAATCCACAAGCACCT+GGG - chr2_2:64390859-64390878 Msa0264440:intron 50.0%
AAAAAGCCCCTCTTGGAGGA+GGG + chr2_2:64391090-64391109 None:intergenic 50.0%
ATGCAAAAAGCCCCTCTTGG+AGG + chr2_2:64391094-64391113 None:intergenic 50.0%
TCTAATCTGCGCAGCTTTCC+TGG - chr2_2:64391233-64391252 Msa0264440:intron 50.0%
TCTAATCTGCGCAGCTTTCC+TGG - chr2_2:64391233-64391252 Msa0264440:intron 50.0%
TCTAATCTGCGCAGCTTTCC+TGG - chr2_2:64391233-64391252 Msa0264440:intron 50.0%
TTAGGACACTTCTGATCGGC+GGG - chr2_2:64391477-64391496 Msa0264440:intron 50.0%
!! ATGAGAAAATGCGGGTTGGC+GGG - chr2_2:64391582-64391601 Msa0264440:CDS 50.0%
TGCTCGATCCCGAAGCTTCT+TGG - chr2_2:64388179-64388198 Msa0264440:three_prime_UTR 55.0%
GCTCGATCCCGAAGCTTCTT+GGG - chr2_2:64388180-64388199 Msa0264440:three_prime_UTR 55.0%
TCCCGAAGCTTCTTGGGACA+AGG - chr2_2:64388186-64388205 Msa0264440:three_prime_UTR 55.0%
TCACCTCCCCAACCCCAAAT+AGG - chr2_2:64389376-64389395 Msa0264440:intron 55.0%
! GATCCTATTTGGGGTTGGGG+AGG + chr2_2:64389382-64389401 None:intergenic 55.0%
TCAGGCAAGCCAGTGGCTTA+TGG + chr2_2:64389564-64389583 None:intergenic 55.0%
ACTAAGTGGGGTCGGTTGCA+TGG + chr2_2:64390075-64390094 None:intergenic 55.0%
ATGCAACCGACCCCACTTAG+TGG - chr2_2:64390074-64390093 Msa0264440:intron 55.0%
TGCAACCGACCCCACTTAGT+GGG - chr2_2:64390075-64390094 Msa0264440:intron 55.0%
CGACCCCACTTAGTGGGATA+AGG - chr2_2:64390081-64390100 Msa0264440:intron 55.0%
CCCAGGTGCTTGTGGATTTG+TGG + chr2_2:64390862-64390881 None:intergenic 55.0%
GAAGAGGAATTTGGCGGCCA+TGG - chr2_2:64391062-64391081 Msa0264440:intron 55.0%
CAAAAAGCCCCTCTTGGAGG+AGG + chr2_2:64391091-64391110 None:intergenic 55.0%
TAGGACACTTCTGATCGGCG+GGG - chr2_2:64391478-64391497 Msa0264440:intron 55.0%
!! ACTTTGGGGCGCCTTTCCAG+TGG - chr2_2:64389457-64389476 Msa0264440:intron 60.0%
CATGGAGCCCTCCTCCAAGA+GGG - chr2_2:64391080-64391099 Msa0264440:intron 60.0%
ATGGAGCCCTCCTCCAAGAG+GGG - chr2_2:64391081-64391100 Msa0264440:intron 60.0%
TTCGGTCTGGCCGGTGCGTA+CGG + chr2_2:64388780-64388799 None:intergenic 65.0%
! TGGGGCGCCTTTCCAGTGGT+TGG - chr2_2:64389461-64389480 Msa0264440:intron 65.0%
GGCGCCTTTCCAGTGGTTGG+AGG - chr2_2:64389464-64389483 Msa0264440:intron 65.0%
CCTCTTGGAGGAGGGCTCCA+TGG + chr2_2:64391082-64391101 None:intergenic 65.0%
CCATGGAGCCCTCCTCCAAG+AGG - chr2_2:64391079-64391098 Msa0264440:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 64387778 64392008 64387778 ID=Msa0264440;Name=Msa0264440
chr2_2 mRNA 64387778 64392008 64387778 ID=Msa0264440-mRNA-1;Parent=Msa0264440;Name=Msa0264440-mRNA-1;_AED=0.05;_eAED=0.05;_QI=328|1|1|1|0.66|0.75|4|517|124
chr2_2 exon 64387778 64388333 64387778 ID=Msa0264440-mRNA-1:exon:20131;Parent=Msa0264440-mRNA-1
chr2_2 exon 64388656 64388785 64388656 ID=Msa0264440-mRNA-1:exon:20130;Parent=Msa0264440-mRNA-1
chr2_2 exon 64390285 64390388 64390285 ID=Msa0264440-mRNA-1:exon:20129;Parent=Msa0264440-mRNA-1
chr2_2 exon 64391579 64392008 64391579 ID=Msa0264440-mRNA-1:exon:20128;Parent=Msa0264440-mRNA-1
chr2_2 five_prime_UTR 64391681 64392008 64391681 ID=Msa0264440-mRNA-1:five_prime_utr;Parent=Msa0264440-mRNA-1
chr2_2 CDS 64391579 64391680 64391579 ID=Msa0264440-mRNA-1:cds;Parent=Msa0264440-mRNA-1
chr2_2 CDS 64390285 64390388 64390285 ID=Msa0264440-mRNA-1:cds;Parent=Msa0264440-mRNA-1
chr2_2 CDS 64388656 64388785 64388656 ID=Msa0264440-mRNA-1:cds;Parent=Msa0264440-mRNA-1
chr2_2 CDS 64388295 64388333 64388295 ID=Msa0264440-mRNA-1:cds;Parent=Msa0264440-mRNA-1
chr2_2 three_prime_UTR 64387778 64388294 64387778 ID=Msa0264440-mRNA-1:three_prime_utr;Parent=Msa0264440-mRNA-1
Gene Sequence

>Msa0264440

ATGAAAGAAGCTAAATCTGTTCCTCAACAACACAACCAGAATGGTCACCTTTCTCCTTTCAAATTCGCCAAATTGCTCGATCCCGAAGCTTCTTGGGACAAGGATCAACTTGGAGATGTATTGCATTGGATTCGACAAGCAATGGGCCTCGTATTTGGATTACTTTGGGGCGCCTTTCCAGTGGTTGGAGGATTTTGGTTTATCCTCTTTTTAGTTTTGTCCTCCGGGATCATATATGGTTACTATTCAGTAATATTGAAGGTTGATGAAGAGGAATTTGGCGGCCATGGAGCCCTCCTCCAAGAGGGGCTTTTTGCATCTACAACTCTTTTTCTGCTTTCCTGGATTCTAGTCTACAGTTTAGGACACTTCTGA
Protein sequence

>Msa0264440

MKEAKSVPQQHNQNGHLSPFKFAKLLDPEASWDKDQLGDVLHWIRQAMGLVFGLLWGAFPVVGGFWFILFLVLSSGIIYGYYSVILKVDEEEFGGHGALLQEGLFASTTLFLLSWILVYSLGHF*