AlfalfaGEDB | Msa0223170

M. sativa cultivar zhongmu-4 / Msa0223170

KEGG: -
GO: -
PFAM: PF07019.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223170	XP_003597363.1	99.194	124	1	0	1	124	1	124	5.34e-82	246

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223170	sp\|Q9CQT9\|RCAF1_MOUSE	37.097	124	77	1	2	124	5	128	2.60e-25	95.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223170	B7FMT6	99.194	124	1	0	1	124	1	124	2.55e-82	246

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0016540	Msa0223170	0.835181	1.948299e-56	-8.615850e-47
Msa0029630	Msa0223170	0.822472	2.326120e-53	-8.615850e-47
Msa0032020	Msa0223170	0.820030	8.506671e-53	-8.615850e-47
Msa0033880	Msa0223170	0.828020	1.133827e-54	-8.615850e-47
Msa0041690	Msa0223170	0.820685	6.019940e-53	-8.615850e-47
Msa0048180	Msa0223170	0.801260	9.791269e-49	-8.615850e-47
Msa0077900	Msa0223170	0.812029	5.207909e-51	-8.615850e-47
Msa0079920	Msa0223170	0.809686	1.674088e-50	-8.615850e-47
Msa0093850	Msa0223170	0.809055	2.285962e-50	-8.615850e-47
Msa0116130	Msa0223170	0.800704	1.272311e-48	-8.615850e-47
Msa0123510	Msa0223170	0.818787	1.633646e-52	-8.615850e-47
Msa0125450	Msa0223170	0.816476	5.423525e-52	-8.615850e-47
Msa0126840	Msa0223170	0.835953	1.242605e-56	-8.615850e-47
Msa0126880	Msa0223170	0.838669	2.507324e-57	-8.615850e-47
Msa0127040	Msa0223170	0.814160	1.775068e-51	-8.615850e-47
Msa0127850	Msa0223170	0.831043	2.087970e-55	-8.615850e-47
Msa0136120	Msa0223170	0.811194	7.910577e-51	-8.615850e-47
Msa0138360	Msa0223170	0.818079	2.363457e-52	-8.615850e-47
Msa0142110	Msa0223170	0.815969	7.038248e-52	-8.615850e-47
Msa0142410	Msa0223170	0.832807	7.656016e-56	-8.615850e-47
Msa0146080	Msa0223170	0.811569	6.557236e-51	-8.615850e-47
Msa0147980	Msa0223170	0.833897	4.096678e-56	-8.615850e-47
Msa0166750	Msa0223170	0.807619	4.626255e-50	-8.615850e-47
Msa0167480	Msa0223170	0.819556	1.091408e-52	-8.615850e-47
Msa0168090	Msa0223170	0.832687	8.198023e-56	-8.615850e-47
Msa0169610	Msa0223170	0.814413	1.560576e-51	-8.615850e-47
Msa0171940	Msa0223170	0.806272	8.917726e-50	-8.615850e-47
Msa0171980	Msa0223170	0.800605	1.332386e-48	-8.615850e-47
Msa0174000	Msa0223170	0.835054	2.097687e-56	-8.615850e-47
Msa0178120	Msa0223170	0.836368	9.751776e-57	-8.615850e-47
Msa0179030	Msa0223170	0.802505	5.435020e-49	-8.615850e-47
Msa0188690	Msa0223170	0.844525	7.163031e-59	-8.615850e-47
Msa0221030	Msa0223170	0.809918	1.492291e-50	-8.615850e-47
Msa0223170	Msa0231100	0.817488	3.212643e-52	-8.615850e-47
Msa0223170	Msa0236620	0.823869	1.098131e-53	-8.615850e-47
Msa0223170	Msa0241830	0.836729	7.889141e-57	-8.615850e-47
Msa0223170	Msa0244970	0.803510	3.368860e-49	-8.615850e-47
Msa0223170	Msa0244980	0.816041	6.783245e-52	-8.615850e-47
Msa0223170	Msa0245580	0.847065	1.463307e-59	-8.615850e-47
Msa0223170	Msa0262480	0.852364	4.846090e-61	-8.615850e-47
Msa0223170	Msa0264440	0.876985	9.029603e-69	-8.615850e-47
Msa0223170	Msa0270210	0.870448	1.447722e-66	-8.615850e-47
Msa0223170	Msa0296250	0.831442	1.665626e-55	-8.615850e-47
Msa0223170	Msa0296920	0.803670	3.121086e-49	-8.615850e-47
Msa0223170	Msa0300350	0.849142	3.908673e-60	-8.615850e-47
Msa0223170	Msa0301520	0.807479	4.953386e-50	-8.615850e-47
Msa0223170	Msa0307850	0.839554	1.478296e-57	-8.615850e-47
Msa0223170	Msa0322900	0.827778	1.296320e-54	-8.615850e-47
Msa0223170	Msa0339690	0.942117	1.267698e-101	-8.615850e-47
Msa0223170	Msa0363060	0.814051	1.875508e-51	-8.615850e-47
Msa0223170	Msa0370160	-0.800724	1.260322e-48	-8.615850e-47
Msa0223170	Msa0384670	0.844142	9.077873e-59	-8.615850e-47
Msa0223170	Msa0423750	0.823376	1.431883e-53	-8.615850e-47
Msa0223170	Msa0434570	0.826787	2.239677e-54	-8.615850e-47
Msa0223170	Msa0434770	0.829510	4.944782e-55	-8.615850e-47
Msa0223170	Msa0436200	0.821092	4.852854e-53	-8.615850e-47
Msa0223170	Msa0443390	0.833477	5.214485e-56	-8.615850e-47
Msa0223170	Msa0474700	0.810307	1.230250e-50	-8.615850e-47
Msa0223170	Msa0478580	0.842418	2.617165e-58	-8.615850e-47
Msa0223170	Msa0511770	0.823230	1.549122e-53	-8.615850e-47
Msa0223170	Msa0518030	0.843791	1.127629e-58	-8.615850e-47
Msa0223170	Msa0524590	0.806419	8.302274e-50	-8.615850e-47
Msa0223170	Msa0531250	0.801527	8.632764e-49	-8.615850e-47
Msa0223170	Msa0531650	0.806566	7.728822e-50	-8.615850e-47
Msa0223170	Msa0534750	0.814390	1.578691e-51	-8.615850e-47
Msa0223170	Msa0542470	0.826331	2.876804e-54	-8.615850e-47
Msa0223170	Msa0547990	0.868902	4.622702e-66	-8.615850e-47
Msa0223170	Msa0550570	0.802634	5.111883e-49	-8.615850e-47
Msa0223170	Msa0581110	0.829385	5.302018e-55	-8.615850e-47
Msa0223170	Msa0583500	0.830496	2.842363e-55	-8.615850e-47
Msa0223170	Msa0593700	0.805521	1.282467e-49	-8.615850e-47
Msa0223170	Msa0615580	0.821158	4.684000e-53	-8.615850e-47
Msa0223170	Msa0619420	0.804390	2.210798e-49	-8.615850e-47
Msa0223170	Msa0633570	0.800511	1.392790e-48	-8.615850e-47
Msa0223170	Msa0644030	0.861088	1.311280e-63	-8.615850e-47
Msa0223170	Msa0656810	0.821384	4.155511e-53	-8.615850e-47
Msa0223170	Msa0658480	0.814697	1.350043e-51	-8.615850e-47
Msa0223170	Msa0659550	0.807264	5.501898e-50	-8.615850e-47
Msa0223170	Msa0662630	0.804102	2.537715e-49	-8.615850e-47
Msa0223170	Msa0666230	0.819225	1.298644e-52	-8.615850e-47
Msa0223170	Msa0669180	0.828740	7.598403e-55	-8.615850e-47
Msa0223170	Msa0687230	0.813542	2.427750e-51	-8.615850e-47
Msa0223170	Msa0687870	0.832763	7.851452e-56	-8.615850e-47
Msa0223170	Msa0694990	0.807488	4.932097e-50	-8.615850e-47
Msa0223170	Msa0744100	0.820442	6.845484e-53	-8.615850e-47
Msa0223170	Msa0751010	0.805094	1.575760e-49	-8.615850e-47
Msa0223170	Msa0785130	0.819405	1.181562e-52	-8.615850e-47
Msa0223170	Msa0788120	0.820700	5.971991e-53	-8.615850e-47
Msa0223170	Msa0790400	0.837978	3.776673e-57	-8.615850e-47
Msa0223170	Msa0794050	0.802190	6.310996e-49	-8.615850e-47
Msa0223170	Msa0808800	0.800017	1.755336e-48	-8.615850e-47
Msa0223170	Msa0819540	0.805910	1.062488e-49	-8.615850e-47
Msa0223170	Msa0825270	0.835650	1.483354e-56	-8.615850e-47
Msa0223170	Msa0826500	0.810835	9.458179e-51	-8.615850e-47
Msa0223170	Msa0826510	0.851549	8.255369e-61	-8.615850e-47
Msa0223170	Msa0864420	0.813738	2.199084e-51	-8.615850e-47
Msa0223170	Msa0865620	0.849239	3.673230e-60	-8.615850e-47
Msa0223170	Msa0867060	0.808013	3.814407e-50	-8.615850e-47
Msa0223170	Msa0892640	0.807026	6.179744e-50	-8.615850e-47
Msa0223170	Msa0943170	0.845364	4.253215e-59	-8.615850e-47
Msa0223170	Msa0970600	0.800780	1.227200e-48	-8.615850e-47
Msa0223170	Msa0981430	0.811753	5.980408e-51	-8.615850e-47
Msa0223170	Msa1019240	0.827231	1.753302e-54	-8.615850e-47
Msa0223170	Msa1019260	0.825521	4.479726e-54	-8.615850e-47
Msa0223170	Msa1030080	0.837514	4.969617e-57	-8.615850e-47
Msa0223170	Msa1070390	0.842068	3.240563e-58	-8.615850e-47
Msa0223170	Msa1079190	0.805270	1.447367e-49	-8.615850e-47
Msa0223170	Msa1083880	0.812745	3.631427e-51	-8.615850e-47
Msa0223170	Msa1104210	0.807735	4.371370e-50	-8.615850e-47
Msa0223170	Msa1123350	0.861393	1.058882e-63	-8.615850e-47
Msa0223170	Msa1126830	0.829394	5.275942e-55	-8.615850e-47
Msa0223170	Msa1127540	0.846735	1.802133e-59	-8.615850e-47
Msa0223170	Msa1127590	0.835705	1.436199e-56	-8.615850e-47
Msa0223170	Msa1131560	0.837825	4.136194e-57	-8.615850e-47
Msa0223170	Msa1133540	0.806338	8.633539e-50	-8.615850e-47
Msa0223170	Msa1145240	0.814755	1.310575e-51	-8.615850e-47
Msa0223170	Msa1158230	0.817632	2.981962e-52	-8.615850e-47
Msa0223170	Msa1165780	0.846025	2.813933e-59	-8.615850e-47
Msa0223170	Msa1166870	0.823910	1.073878e-53	-8.615850e-47
Msa0223170	Msa1169080	0.808568	2.905981e-50	-8.615850e-47
Msa0223170	Msa1179980	0.804880	1.747187e-49	-8.615850e-47
Msa0223170	Msa1194230	0.821308	4.325738e-53	-8.615850e-47
Msa0223170	Msa1201830	0.820759	5.788027e-53	-8.615850e-47
Msa0223170	Msa1206650	0.825884	3.674724e-54	-8.615850e-47
Msa0223170	Msa1208020	0.824980	6.013319e-54	-8.615850e-47
Msa0223170	Msa1214320	0.803413	3.528445e-49	-8.615850e-47
Msa0223170	Msa1227690	0.822432	2.376818e-53	-8.615850e-47
Msa0223170	Msa1255410	0.812366	4.396153e-51	-8.615850e-47
Msa0223170	Msa1262890	0.801797	7.600814e-49	-8.615850e-47
Msa0223170	Msa1266060	0.805578	1.247833e-49	-8.615850e-47
Msa0223170	Msa1270620	0.819372	1.202045e-52	-8.615850e-47
Msa0223170	Msa1270690	0.807010	6.229237e-50	-8.615850e-47
Msa0223170	Msa1276970	0.826089	3.284337e-54	-8.615850e-47
Msa0223170	Msa1290450	0.817487	3.214743e-52	-8.615850e-47
Msa0223170	Msa1294840	0.816457	5.476309e-52	-8.615850e-47
Msa0223170	Msa1329300	0.803821	2.903348e-49	-8.615850e-47
Msa0223170	Msa1331250	0.821269	4.415864e-53	-8.615850e-47
Msa0223170	Msa1333560	0.807023	6.187358e-50	-8.615850e-47
Msa0223170	Msa1343290	0.812709	3.699367e-51	-8.615850e-47
Msa0223170	Msa1344310	0.831311	1.793930e-55	-8.615850e-47
Msa0223170	Msa1351540	0.820413	6.950090e-53	-8.615850e-47
Msa0223170	Msa1356610	0.836203	1.074056e-56	-8.615850e-47
Msa0223170	Msa1371520	0.801020	1.096578e-48	-8.615850e-47
Msa0223170	Msa1373170	0.849620	2.877677e-60	-8.615850e-47
Msa0223170	Msa1373820	0.815097	1.100934e-51	-8.615850e-47
Msa0223170	Msa1376650	0.802952	4.395136e-49	-8.615850e-47
Msa0223170	Msa1381350	0.807701	4.444700e-50	-8.615850e-47
Msa0223170	Msa1391480	0.831206	1.903295e-55	-8.615850e-47
Msa0223170	Msa1398790	0.825833	3.777329e-54	-8.615850e-47
Msa0223170	Msa1403570	0.860238	2.374155e-63	-8.615850e-47
Msa0223170	Msa1409260	0.800122	1.671513e-48	-8.615850e-47
Msa0223170	Msa1410440	0.801263	9.779653e-49	-8.615850e-47
Msa0223170	Msa1411500	0.827434	1.567690e-54	-8.615850e-47
Msa0223170	Msa1411950	0.821160	4.680870e-53	-8.615850e-47
Msa0223170	Msa1412660	0.804297	2.311033e-49	-8.615850e-47
Msa0223170	Msa1418500	0.802494	5.461728e-49	-8.615850e-47
Msa0223170	Msa1427160	0.995314	4.808614e-215	-8.615850e-47
Msa0223170	Msa1432480	0.833432	5.352066e-56	-8.615850e-47
Msa0223170	Msa1443300	0.836495	9.051364e-57	-8.615850e-47
Msa0223170	Msa1458810	0.804503	2.093484e-49	-8.615850e-47
Msa0223170	Msa1459910	0.850907	1.253487e-60	-8.615850e-47
Msa0223170	Msa1459960	0.862838	3.815670e-64	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223170	MtrunA17_Chr2g0328321	99.194	124	1	0	1	124	1	124	4.91e-86	246

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223170	AT5G59410.1	73.077	130	29	1	1	124	1	130	5.20e-66	196
Msa0223170	AT2G29020.1	71.795	117	33	0	8	124	17	133	6.15e-52	160

Find 85 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGCGGTGAAATTGGGAATT+AGG	0.168597	2_1:-72716851	None:intergenic
CACACGTTTGTGGCAAATTA+TGG	0.174659	2_1:+72720605	Msa0223170:three_prime_UTR
TTCCAGTGGTTGGAGGATTT+TGG	0.190036	2_1:+72718381	Msa0223170:CDS
TTTATAATCAAAGTATTTAA+TGG	0.206914	2_1:-72720798	None:intergenic
CAAGCAATGGGCCTCGTATT+TGG	0.214066	2_1:+72718341	Msa0223170:CDS
CCTCGTATTTGGATTACTTT+GGG	0.235564	2_1:+72718352	Msa0223170:CDS
TCTAATCTGCGCAGCTTTCC+TGG	0.257466	2_1:+72720349	Msa0223170:intron
TGCTCGATCCCGAAGCTTCT+TGG	0.267462	2_1:+72717089	Msa0223170:CDS
TATTGACCCGTGTTTAATAA+AGG	0.296142	2_1:+72720660	Msa0223170:three_prime_UTR
TTTATATTTGATTTCATGTT+TGG	0.298071	2_1:+72720450	Msa0223170:three_prime_UTR
TTGTCCTCCGGGATCATATA+TGG	0.299115	2_1:+72719921	Msa0223170:CDS
TTATAAGATAAAATAAATAA+TGG	0.304898	2_1:-72716947	None:intergenic
AACGGCAAGAGCGGTGAAAT+TGG	0.307608	2_1:-72716859	None:intergenic
AGCTTCGGGATCGAGCAATT+TGG	0.310638	2_1:-72717083	None:intergenic
AGCGGTGAAATTGGGAATTA+GGG	0.318885	2_1:-72716850	None:intergenic
AAGGTTGATGAAGAGGAATT+TGG	0.323605	2_1:+72719963	Msa0223170:CDS
TGGATTCTAGTCTACAGTTT+AGG	0.323678	2_1:+72720369	Msa0223170:CDS
TCTTCACTCTCTTTGTTACT+TGG	0.330250	2_1:+72716797	Msa0223170:five_prime_UTR
TGTTTAATAAAGGTGCTTGC+AGG	0.378128	2_1:+72720670	Msa0223170:three_prime_UTR
GCTCGATCCCGAAGCTTCTT+GGG	0.378769	2_1:+72717090	Msa0223170:CDS
GGAATTAGGGTTTGGTAAAA+CGG	0.379819	2_1:-72716837	None:intergenic
AACCTTGTCCCAAGAAGCTT+CGG	0.386161	2_1:-72717098	None:intergenic
GCCTCGTATTTGGATTACTT+TGG	0.386345	2_1:+72718351	Msa0223170:CDS
GCATCAGAGAGACAAAATAT+TGG	0.394751	2_1:-72720773	None:intergenic
TGAATTAATTAGTGTAAAGT+AGG	0.398454	2_1:-72716920	None:intergenic
AAGGAGAAAGGTGACCATTC+TGG	0.409042	2_1:-72717051	None:intergenic
GTGAAGAATTAGAAGATGAC+TGG	0.409526	2_1:-72716781	None:intergenic
AATTAAGTTCCAGTGAGTTA+TGG	0.410416	2_1:-72716757	None:intergenic
TTTGAATCATAAGGTAGATT+GGG	0.412860	2_1:-72716893	None:intergenic
TGAAATTGGGAATTAGGGTT+TGG	0.420838	2_1:-72716845	None:intergenic
TATAAGATAAAATAAATAAT+GGG	0.427970	2_1:-72716946	None:intergenic
TCATTGATTTCAGAACACTA+TGG	0.428967	2_1:-72720473	None:intergenic
CATCAGAGAGACAAAATATT+GGG	0.432128	2_1:-72720772	None:intergenic
TGGGGCGCCTTTCCAGTGGT+TGG	0.443020	2_1:+72718371	Msa0223170:CDS
TTAATGACACCACACGTTTG+TGG	0.447372	2_1:+72720595	Msa0223170:three_prime_UTR
ACGGCAAGAGCGGTGAAATT+GGG	0.458037	2_1:-72716858	None:intergenic
TGGCGAATTTGAAAGGAGAA+AGG	0.461188	2_1:-72717063	None:intergenic
GAAGAGGAATTTGGCGGCCA+TGG	0.466186	2_1:+72719972	Msa0223170:CDS
GAATTAATTAGTGTAAAGTA+GGG	0.468340	2_1:-72716919	None:intergenic
GAATTAGGGTTTGGTAAAAC+GGG	0.470212	2_1:-72716836	None:intergenic
TTAGGACACTTCTGATCGGC+GGG	0.474638	2_1:+72720387	Msa0223170:exon
CCTCTTGGAGGAGGGCTCCA+TGG	0.480198	2_1:-72719989	None:intergenic
AGCAATTTGGCGAATTTGAA+AGG	0.482080	2_1:-72717070	None:intergenic
ATGAGAAAATGCGGGTTGGC+GGG	0.484750	2_1:+72720492	Msa0223170:three_prime_UTR
TTACTATTCAGTAATATTGA+AGG	0.488058	2_1:+72719944	Msa0223170:CDS
CATTGGATTCGACAAGCAAT+GGG	0.488187	2_1:+72718329	Msa0223170:CDS
AACTTGGAGATGTATTGCAT+TGG	0.493240	2_1:+72718312	Msa0223170:CDS
GCATTGGATTCGACAAGCAA+TGG	0.498506	2_1:+72718328	Msa0223170:CDS
ACCTTGTCCCAAGAAGCTTC+GGG	0.501872	2_1:-72717097	None:intergenic
CATGGAGCCCTCCTCCAAGA+GGG	0.507327	2_1:+72719990	Msa0223170:CDS
CCATTCTGGTGGTGTTGTTG+AGG	0.511709	2_1:-72717037	None:intergenic
TAAACTGTAGACTAGAATCC+AGG	0.517183	2_1:-72720367	None:intergenic
AACCAAAATCCTCCAACCAC+TGG	0.518282	2_1:-72718383	None:intergenic
GGCGCCTTTCCAGTGGTTGG+AGG	0.519426	2_1:+72718374	Msa0223170:CDS
AATGAGAAAATGCGGGTTGG+CGG	0.525036	2_1:+72720491	Msa0223170:three_prime_UTR
AATATTGAAGGTTGATGAAG+AGG	0.533923	2_1:+72719956	Msa0223170:CDS
ATGGAGCCCTCCTCCAAGAG+GGG	0.538216	2_1:+72719991	Msa0223170:CDS
TGAAGAATTAGAAGATGACT+GGG	0.540663	2_1:-72716780	None:intergenic
GATTGGGTAACTACGAGAAA+CGG	0.544095	2_1:-72716877	None:intergenic
TCCCGAAGCTTCTTGGGACA+AGG	0.549633	2_1:+72717096	Msa0223170:CDS
GTTGATGAAGAGGAATTTGG+CGG	0.560236	2_1:+72719966	Msa0223170:CDS
AACCCCAAATAGGATCAACT+TGG	0.560316	2_1:+72718296	Msa0223170:intron
CTCGTATTTGGATTACTTTG+GGG	0.563337	2_1:+72718353	Msa0223170:CDS
CTGAAATCAATGAGAAAATG+CGG	0.568466	2_1:+72720483	Msa0223170:three_prime_UTR
TGAAATCAATGAGAAAATGC+GGG	0.575073	2_1:+72720484	Msa0223170:three_prime_UTR
CCTCAACAACACCACCAGAA+TGG	0.583456	2_1:+72717037	Msa0223170:CDS
AAATCCTCCAACCACTGGAA+AGG	0.583783	2_1:-72718378	None:intergenic
ATAATCAAAGTATTTAATGG+AGG	0.593603	2_1:-72720795	None:intergenic
CCATGGAGCCCTCCTCCAAG+AGG	0.596639	2_1:+72719989	Msa0223170:CDS
TTAATATCATAATTCAAGTG+TGG	0.597042	2_1:-72720827	None:intergenic
ACTTTGGGGCGCCTTTCCAG+TGG	0.601275	2_1:+72718367	Msa0223170:CDS
TGCTTGCAGGCAAATTCTGA+AGG	0.601769	2_1:+72720683	Msa0223170:three_prime_UTR
GCAAGCACCTTTATTAAACA+CGG	0.602743	2_1:-72720667	None:intergenic
ATCAATGAGAAAATGCGGGT+TGG	0.603593	2_1:+72720488	Msa0223170:three_prime_UTR
ACTACGAGAAACGGCAAGAG+CGG	0.607619	2_1:-72716868	None:intergenic
GATATCTTCAGAATTTGCAA+AGG	0.610174	2_1:-72720709	None:intergenic
ATAGTAACCATATATGATCC+CGG	0.618972	2_1:-72719928	None:intergenic
CATAATTTGCCACAAACGTG+TGG	0.620918	2_1:-72720604	None:intergenic
CAGTTTAGGACACTTCTGAT+CGG	0.623936	2_1:+72720383	Msa0223170:exon
TTTAGGACACTTCTGATCGG+CGG	0.628007	2_1:+72720386	Msa0223170:exon
CCCAAAGTAATCCAAATACG+AGG	0.634018	2_1:-72718352	None:intergenic
CAAGCACCTTTATTAAACAC+GGG	0.647466	2_1:-72720666	None:intergenic
GAGAAAGGTGACCATTCTGG+TGG	0.680555	2_1:-72717048	None:intergenic
TAGGACACTTCTGATCGGCG+GGG	0.755597	2_1:+72720388	Msa0223170:exon
GTAACCATATATGATCCCGG+AGG	0.792581	2_1:-72719925	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTATATAAACTATGT+GGG	-	chr2_1:72718076-72718095	None:intergenic	10.0%
!!!	TTTTTTTTATATAAACTATG+TGG	-	chr2_1:72718077-72718096	None:intergenic	10.0%
!!!	TTTATAATCAAAGTATTTAA+TGG	-	chr2_1:72720801-72720820	None:intergenic	10.0%
!!!	TTATTTTTGAGTAAAAAAAG+TGG	+	chr2_1:72717217-72717236	Msa0223170:intron	15.0%
!!!	TATTTTTGAGTAAAAAAAGT+GGG	+	chr2_1:72717218-72717237	Msa0223170:intron	15.0%
!!!	TGGGTTTTATTAATATATTA+TGG	+	chr2_1:72717237-72717256	Msa0223170:intron	15.0%
!!	TAATTGATTATATTTAGACA+CGG	+	chr2_1:72717382-72717401	Msa0223170:intron	15.0%
!!	AATAAAATTTACACATATAG+GGG	-	chr2_1:72718757-72718776	None:intergenic	15.0%
!!	GAATAAAATTTACACATATA+GGG	-	chr2_1:72718758-72718777	None:intergenic	15.0%
!!	TGAATAAAATTTACACATAT+AGG	-	chr2_1:72718759-72718778	None:intergenic	15.0%
!!	TGATATATGATAAAACATTA+TGG	+	chr2_1:72718948-72718967	Msa0223170:intron	15.0%
!!!	TACTATTTGTTTTCATTTTT+TGG	+	chr2_1:72719040-72719059	Msa0223170:intron	15.0%
!!!	ATAAATTTTCTAATACATAC+TGG	-	chr2_1:72719145-72719164	None:intergenic	15.0%
!!	ATTACCTAAAATTAAGTTAA+GGG	-	chr2_1:72719437-72719456	None:intergenic	15.0%
!!!	TTTATATTTGATTTCATGTT+TGG	+	chr2_1:72720450-72720469	Msa0223170:three_prime_UTR	15.0%
!!	GAATTAATTAGTGTAAAGTA+GGG	-	chr2_1:72716922-72716941	None:intergenic	20.0%
!!	TGAATTAATTAGTGTAAAGT+AGG	-	chr2_1:72716923-72716942	None:intergenic	20.0%
!!	CTCAAAAATAAACAAAATCT+GGG	-	chr2_1:72717208-72717227	None:intergenic	20.0%
!!	ACTCAAAAATAAACAAAATC+TGG	-	chr2_1:72717209-72717228	None:intergenic	20.0%
!!	AAAACTTGAGAGTAAATTAA+TGG	-	chr2_1:72717279-72717298	None:intergenic	20.0%
!!	ATTACTCATCTAATTAAACA+TGG	-	chr2_1:72717347-72717366	None:intergenic	20.0%
!!	AATGAATCATGAACAAATTA+AGG	-	chr2_1:72717501-72717520	None:intergenic	20.0%
!!	TACGGAAAAATATATTTGTT+GGG	-	chr2_1:72717672-72717691	None:intergenic	20.0%
!!	AAAACTAAATGGTTAATCTA+GGG	-	chr2_1:72717986-72718005	None:intergenic	20.0%
!!!	GTATTGACATTAAATTATGT+AGG	-	chr2_1:72718580-72718599	None:intergenic	20.0%
!!	ATAAAATTTACACATATAGG+GGG	-	chr2_1:72718756-72718775	None:intergenic	20.0%
!!!	TGTAAATTTTATTCAGAACT+TGG	+	chr2_1:72718764-72718783	Msa0223170:intron	20.0%
!!	TTACCTAAAATTAAGTTAAG+GGG	-	chr2_1:72719436-72719455	None:intergenic	20.0%
!!	CATTACCTAAAATTAAGTTA+AGG	-	chr2_1:72719438-72719457	None:intergenic	20.0%
!!!	TTTTTTTCTTCACTTCATTT+TGG	+	chr2_1:72719477-72719496	Msa0223170:intron	20.0%
!!!	TTTTTTTTTCCACATCATTT+TGG	-	chr2_1:72719652-72719671	None:intergenic	20.0%
!!!	AATGTGTACTATTTTAAACT+AGG	-	chr2_1:72719727-72719746	None:intergenic	20.0%
!!!	AGTACACATTATGTTTTTAT+TGG	+	chr2_1:72719737-72719756	Msa0223170:intron	20.0%
!!	TTACTATTCAGTAATATTGA+AGG	+	chr2_1:72719944-72719963	Msa0223170:CDS	20.0%
!!!	GCAAATTTAATTTTTTGTGT+GGG	-	chr2_1:72720089-72720108	None:intergenic	20.0%
!!!	AGCAAATTTAATTTTTTGTG+TGG	-	chr2_1:72720090-72720109	None:intergenic	20.0%
!!!	GCAAATTTAATTTTTTGTGT+GGG	-	chr2_1:72720089-72720108	None:intergenic	20.0%
!!!	AGCAAATTTAATTTTTTGTG+TGG	-	chr2_1:72720090-72720109	None:intergenic	20.0%
!!!	GCAAATTTAATTTTTTGTGT+GGG	-	chr2_1:72720089-72720108	None:intergenic	20.0%
!!!	AGCAAATTTAATTTTTTGTG+TGG	-	chr2_1:72720090-72720109	None:intergenic	20.0%
!!!	ATAATCAAAGTATTTAATGG+AGG	-	chr2_1:72720798-72720817	None:intergenic	20.0%
!!	TTAATATCATAATTCAAGTG+TGG	-	chr2_1:72720830-72720849	None:intergenic	20.0%
!!!	TTTTATTTTCCATAACTCAC+TGG	+	chr2_1:72716748-72716767	Msa0223170:five_prime_UTR	25.0%
!	TTTGAATCATAAGGTAGATT+GGG	-	chr2_1:72716896-72716915	None:intergenic	25.0%
!!	TTTTGAATCATAAGGTAGAT+TGG	-	chr2_1:72716897-72716916	None:intergenic	25.0%
!!!	GACTTTTTTTTCTTTAGAGT+TGG	+	chr2_1:72717431-72717450	Msa0223170:intron	25.0%
!	TCATGATTCATTGATATTGT+TGG	+	chr2_1:72717509-72717528	Msa0223170:intron	25.0%
!	GTACGGAAAAATATATTTGT+TGG	-	chr2_1:72717673-72717692	None:intergenic	25.0%
!	AAACTACACTATAATCAAAC+GGG	-	chr2_1:72717868-72717887	None:intergenic	25.0%
!	GAAACTACACTATAATCAAA+CGG	-	chr2_1:72717869-72717888	None:intergenic	25.0%
!!	TATAGTGTAGTTTCTTCTTT+TGG	+	chr2_1:72717875-72717894	Msa0223170:intron	25.0%
!!	ATAGTGTAGTTTCTTCTTTT+GGG	+	chr2_1:72717876-72717895	Msa0223170:intron	25.0%
!	GAAAACTAAATGGTTAATCT+AGG	-	chr2_1:72717987-72718006	None:intergenic	25.0%
!	ACAAATCAGTGAAAACTAAA+TGG	-	chr2_1:72717997-72718016	None:intergenic	25.0%
!!!	TTAGTTTTCACTGATTTGTT+TGG	+	chr2_1:72717998-72718017	Msa0223170:intron	25.0%
!	TTAAGATAAACATACAGGAT+CGG	-	chr2_1:72718431-72718450	None:intergenic	25.0%
!!!	CAACTTTAAGATAAACATAC+AGG	-	chr2_1:72718436-72718455	None:intergenic	25.0%
!!!	AAGATGTGCTATTTTTTAAG+TGG	-	chr2_1:72718817-72718836	None:intergenic	25.0%
!	TGTCAACTAATGAGTTATTT+AGG	+	chr2_1:72718921-72718940	Msa0223170:intron	25.0%
!	GTCAACTAATGAGTTATTTA+GGG	+	chr2_1:72718922-72718941	Msa0223170:intron	25.0%
!	AAAATGTGACTTAAGTTACA+TGG	+	chr2_1:72719079-72719098	Msa0223170:intron	25.0%
!	TGTATACAGTATATCTAGAA+AGG	-	chr2_1:72719180-72719199	None:intergenic	25.0%
!	AATAACTAAACAAGCATCTA+AGG	-	chr2_1:72719315-72719334	None:intergenic	25.0%
!!!	AGTACTGGTTATATTTTGAT+TGG	-	chr2_1:72719340-72719359	None:intergenic	25.0%
!	TTGAAATACATATCCAAACA+TGG	-	chr2_1:72719512-72719531	None:intergenic	25.0%
!!!	AAATCTAAGGTTTTCTTTTG+GGG	-	chr2_1:72719592-72719611	None:intergenic	25.0%
!!!	CAAATCTAAGGTTTTCTTTT+GGG	-	chr2_1:72719593-72719612	None:intergenic	25.0%
!!!	TCAAATCTAAGGTTTTCTTT+TGG	-	chr2_1:72719594-72719613	None:intergenic	25.0%
	AATTAAGTTCCAGTGAGTTA+TGG	-	chr2_1:72716760-72716779	None:intergenic	30.0%
	TGAAGAATTAGAAGATGACT+GGG	-	chr2_1:72716783-72716802	None:intergenic	30.0%
!	GTAGGGATTTTTGAATCATA+AGG	-	chr2_1:72716905-72716924	None:intergenic	30.0%
	AGATCGAAAAACATGAAAAC+AGG	-	chr2_1:72717143-72717162	None:intergenic	30.0%
!!!	AGGTTGTTTTTTCAGGAAAT+AGG	+	chr2_1:72717168-72717187	Msa0223170:intron	30.0%
	TAAATGCAACCAACATAGTT+TGG	-	chr2_1:72717745-72717764	None:intergenic	30.0%
	GATCATAGATAATGAGATCA+TGG	-	chr2_1:72717784-72717803	None:intergenic	30.0%
	CAAAACTGAATGCTTAATCA+AGG	-	chr2_1:72717816-72717835	None:intergenic	30.0%
!!!	ATTCAGTTTTGCTGATTTGT+TGG	+	chr2_1:72717825-72717844	Msa0223170:intron	30.0%
!!!	TTCAGTTTTGCTGATTTGTT+GGG	+	chr2_1:72717826-72717845	Msa0223170:intron	30.0%
	AACTACACTATAATCAAACG+GGG	-	chr2_1:72717867-72717886	None:intergenic	30.0%
	CATGTAGTATGTGATTGATA+TGG	+	chr2_1:72718123-72718142	Msa0223170:intron	30.0%
	ATTGTGGAAGATACTTTAGA+AGG	+	chr2_1:72718161-72718180	Msa0223170:intron	30.0%
	AAAGATAAAAACAGAGAAGC+TGG	-	chr2_1:72718267-72718286	None:intergenic	30.0%
	TCGGTAAAATAAACACTTAC+AGG	-	chr2_1:72718412-72718431	None:intergenic	30.0%
	ATCAGTGAATATGAGAATTC+TGG	-	chr2_1:72718510-72718529	None:intergenic	30.0%
	TCGTGCATAAACAACTTTAT+TGG	+	chr2_1:72718539-72718558	Msa0223170:intron	30.0%
	CGTGCATAAACAACTTTATT+GGG	+	chr2_1:72718540-72718559	Msa0223170:intron	30.0%
!	ATTATCTGAAATACCAATGC+TGG	+	chr2_1:72718619-72718638	Msa0223170:intron	30.0%
!	AACAAAATTTTCTCCAGCAT+TGG	-	chr2_1:72718635-72718654	None:intergenic	30.0%
!	AATGCTGGAGAAAATTTTGT+TGG	+	chr2_1:72718634-72718653	Msa0223170:intron	30.0%
	ATGACAACTTGTAAGAACAA+AGG	-	chr2_1:72718663-72718682	None:intergenic	30.0%
	CTAAGTGTGATGCAAATATT+GGG	-	chr2_1:72718686-72718705	None:intergenic	30.0%
!	TGCTCTAAGTACAGAAATTA+GGG	+	chr2_1:72718728-72718747	Msa0223170:intron	30.0%
	GGTCATGAAATCAAAAACAA+TGG	+	chr2_1:72718785-72718804	Msa0223170:intron	30.0%
	CATGAAATCAAAAACAATGG+AGG	+	chr2_1:72718788-72718807	Msa0223170:intron	30.0%
	GATATAAAAACGAAGAACTG+AGG	-	chr2_1:72718844-72718863	None:intergenic	30.0%
!	TGATCGTCTTTTGCATAAAT+TGG	+	chr2_1:72719108-72719127	Msa0223170:intron	30.0%
!!!	GTGAAATTTTTTGTCCAGAA+GGG	+	chr2_1:72719231-72719250	Msa0223170:intron	30.0%
	TCTCAGTCTTAAGTATATGT+TGG	+	chr2_1:72719565-72719584	Msa0223170:intron	30.0%
	CTCAGTCTTAAGTATATGTT+GGG	+	chr2_1:72719566-72719585	Msa0223170:intron	30.0%
	ATTCTCAAGCTTCAAATCTA+AGG	-	chr2_1:72719605-72719624	None:intergenic	30.0%
	TGAGAATCCATATACAAACA+TGG	+	chr2_1:72719618-72719637	Msa0223170:intron	30.0%
!	CTTAATTTCAGGTAACGATT+TGG	-	chr2_1:72719680-72719699	None:intergenic	30.0%
	TCGTTACCTGAAATTAAGTT+TGG	+	chr2_1:72719682-72719701	Msa0223170:intron	30.0%
	CGTTACCTGAAATTAAGTTT+GGG	+	chr2_1:72719683-72719702	Msa0223170:intron	30.0%
!	AAACTTTTCATTGCACTAGT+AGG	+	chr2_1:72719813-72719832	Msa0223170:intron	30.0%
	AAAAAGCTGCAATACAAACA+GGG	-	chr2_1:72719897-72719916	None:intergenic	30.0%
	TAAAAAGCTGCAATACAAAC+AGG	-	chr2_1:72719898-72719917	None:intergenic	30.0%
	ATAGTAACCATATATGATCC+CGG	-	chr2_1:72719931-72719950	None:intergenic	30.0%
!!	AATATTGAAGGTTGATGAAG+AGG	+	chr2_1:72719956-72719975	Msa0223170:CDS	30.0%
	AGAAAAGAAGTCTTAGATTG+TGG	-	chr2_1:72720126-72720145	None:intergenic	30.0%
	AAACTTTCTACTGTTAATCC+AGG	-	chr2_1:72720164-72720183	None:intergenic	30.0%
	AGAAAAGAAGTCTTAGATTG+TGG	-	chr2_1:72720126-72720145	None:intergenic	30.0%
	AAACTTTCTACTGTTAATCC+AGG	-	chr2_1:72720164-72720183	None:intergenic	30.0%
	AGAAAAGAAGTCTTAGATTG+TGG	-	chr2_1:72720126-72720145	None:intergenic	30.0%
!!	TCATTGATTTCAGAACACTA+TGG	-	chr2_1:72720476-72720495	None:intergenic	30.0%
	CTGAAATCAATGAGAAAATG+CGG	+	chr2_1:72720483-72720502	Msa0223170:three_prime_UTR	30.0%
	TGAAATCAATGAGAAAATGC+GGG	+	chr2_1:72720484-72720503	Msa0223170:three_prime_UTR	30.0%
!	AAAACATTCTGATTTTGTCG+AGG	+	chr2_1:72720532-72720551	Msa0223170:three_prime_UTR	30.0%
!	AAACATTCTGATTTTGTCGA+GGG	+	chr2_1:72720533-72720552	Msa0223170:three_prime_UTR	30.0%
	TATTGACCCGTGTTTAATAA+AGG	+	chr2_1:72720660-72720679	Msa0223170:three_prime_UTR	30.0%
	GATATCTTCAGAATTTGCAA+AGG	-	chr2_1:72720712-72720731	None:intergenic	30.0%
	CATCAGAGAGACAAAATATT+GGG	-	chr2_1:72720775-72720794	None:intergenic	30.0%
!!!	ATTAGTTTTGCACTATGTTG+AGG	+	chr2_1:72720865-72720884	Msa0223170:three_prime_UTR	30.0%
!!!	TTAGTTTTGCACTATGTTGA+GGG	+	chr2_1:72720866-72720885	Msa0223170:three_prime_UTR	30.0%
	GTGAAGAATTAGAAGATGAC+TGG	-	chr2_1:72716784-72716803	None:intergenic	35.0%
	TCTTCACTCTCTTTGTTACT+TGG	+	chr2_1:72716797-72716816	Msa0223170:five_prime_UTR	35.0%
!	GAATTAGGGTTTGGTAAAAC+GGG	-	chr2_1:72716839-72716858	None:intergenic	35.0%
!	GGAATTAGGGTTTGGTAAAA+CGG	-	chr2_1:72716840-72716859	None:intergenic	35.0%
	TGAAATTGGGAATTAGGGTT+TGG	-	chr2_1:72716848-72716867	None:intergenic	35.0%
	AGCAATTTGGCGAATTTGAA+AGG	-	chr2_1:72717073-72717092	None:intergenic	35.0%
!!	CATGTTTTTCGATCTGTTTG+AGG	+	chr2_1:72717148-72717167	Msa0223170:intron	35.0%
!!!	CTGTTTGAGGTTGTTTTTTC+AGG	+	chr2_1:72717161-72717180	Msa0223170:intron	35.0%
	CGGTATAAGTATCAGTGATT+TGG	+	chr2_1:72717402-72717421	Msa0223170:intron	35.0%
	AGAGAGTGAGATCCATTTAA+GGG	+	chr2_1:72717636-72717655	Msa0223170:intron	35.0%
!	ATATATTTTTCCGTACGCAC+CGG	+	chr2_1:72717677-72717696	Msa0223170:intron	35.0%
	ACTTGGGTCTCTTAAGTATT+CGG	-	chr2_1:72717708-72717727	None:intergenic	35.0%
	TGCATTTAGCTGATATGAGT+TGG	+	chr2_1:72717757-72717776	Msa0223170:intron	35.0%
!!!	TTTTGCTGATTTGTTGGGTT+CGG	+	chr2_1:72717831-72717850	Msa0223170:intron	35.0%
!!	ATTTAACAGTGGTTCTGAGT+TGG	+	chr2_1:72717928-72717947	Msa0223170:intron	35.0%
	ACTAAATGGTTAATCTAGGG+TGG	-	chr2_1:72717983-72718002	None:intergenic	35.0%
	GATGTAGCTTCTCTAAATTG+TGG	+	chr2_1:72718145-72718164	Msa0223170:intron	35.0%
!	TTTTGCACTTCTCATTCTAG+AGG	+	chr2_1:72718207-72718226	Msa0223170:intron	35.0%
	TAAAAACAGAGAAGCTGGTT+AGG	-	chr2_1:72718262-72718281	None:intergenic	35.0%
!!	TCTCCAAGTTGATCCTATTT+GGG	-	chr2_1:72718302-72718321	None:intergenic	35.0%
!!	ATCTCCAAGTTGATCCTATT+TGG	-	chr2_1:72718303-72718322	None:intergenic	35.0%
	AACTTGGAGATGTATTGCAT+TGG	+	chr2_1:72718312-72718331	Msa0223170:CDS	35.0%
	CCTCGTATTTGGATTACTTT+GGG	+	chr2_1:72718352-72718371	Msa0223170:CDS	35.0%
	CTCGTATTTGGATTACTTTG+GGG	+	chr2_1:72718353-72718372	Msa0223170:CDS	35.0%
	TCTGGAAAGACTAAATCATC+AGG	-	chr2_1:72718492-72718511	None:intergenic	35.0%
	CCTAAGTGTGATGCAAATAT+TGG	-	chr2_1:72718687-72718706	None:intergenic	35.0%
	CCAATATTTGCATCACACTT+AGG	+	chr2_1:72718684-72718703	Msa0223170:intron	35.0%
!	CTGCTCTAAGTACAGAAATT+AGG	+	chr2_1:72718727-72718746	Msa0223170:intron	35.0%
	CCTTGCAAAGCAAAATTTGA+TGG	-	chr2_1:72719212-72719231	None:intergenic	35.0%
!	CCATCAAATTTTGCTTTGCA+AGG	+	chr2_1:72719209-72719228	Msa0223170:intron	35.0%
!!!	GGTGAAATTTTTTGTCCAGA+AGG	+	chr2_1:72719230-72719249	Msa0223170:intron	35.0%
	AAAGACAAATGATGAAGGTG+AGG	-	chr2_1:72719260-72719279	None:intergenic	35.0%
	TTGGGAAAGACAAATGATGA+AGG	-	chr2_1:72719265-72719284	None:intergenic	35.0%
!!	TAGAGTTTTGTCGTAAGTCT+GGG	-	chr2_1:72719288-72719307	None:intergenic	35.0%
!!	ATAGAGTTTTGTCGTAAGTC+TGG	-	chr2_1:72719289-72719308	None:intergenic	35.0%
	TGAGGTGTAAAATACAGTAC+TGG	-	chr2_1:72719355-72719374	None:intergenic	35.0%
	ATGTGATGATCTCACATTTG+AGG	-	chr2_1:72719373-72719392	None:intergenic	35.0%
	ACAAGTGTGAGGAAGTTAAA+AGG	+	chr2_1:72719393-72719412	Msa0223170:intron	35.0%
!	CTGCCCCTTAACTTAATTTT+AGG	+	chr2_1:72719430-72719449	Msa0223170:intron	35.0%
	CATATCCAAACATGGATAGA+AGG	-	chr2_1:72719504-72719523	None:intergenic	35.0%
	CCTTCATCCATGTTTGTATA+TGG	-	chr2_1:72719628-72719647	None:intergenic	35.0%
	CCATATACAAACATGGATGA+AGG	+	chr2_1:72719625-72719644	Msa0223170:intron	35.0%
!	TGAAATTAAGTTTGGGACCA+CGG	+	chr2_1:72719690-72719709	Msa0223170:intron	35.0%
	AAACAGGGAAACTCTACTTT+AGG	-	chr2_1:72719882-72719901	None:intergenic	35.0%
!!!	AGCTTTTTAGTTTTGTCCTC+CGG	+	chr2_1:72719909-72719928	Msa0223170:intron	35.0%
!!	AAGGTTGATGAAGAGGAATT+TGG	+	chr2_1:72719963-72719982	Msa0223170:CDS	35.0%
!	TGCATCTACAACTCTTTTTC+TGG	+	chr2_1:72720019-72720038	Msa0223170:CDS	35.0%
	TAAACTGTAGACTAGAATCC+AGG	-	chr2_1:72720370-72720389	None:intergenic	35.0%
	TGGATTCTAGTCTACAGTTT+AGG	+	chr2_1:72720369-72720388	Msa0223170:CDS	35.0%
	CAAGCACCTTTATTAAACAC+GGG	-	chr2_1:72720669-72720688	None:intergenic	35.0%
	GCAAGCACCTTTATTAAACA+CGG	-	chr2_1:72720670-72720689	None:intergenic	35.0%
!!	TGTTTAATAAAGGTGCTTGC+AGG	+	chr2_1:72720670-72720689	Msa0223170:three_prime_UTR	35.0%
	GCATCAGAGAGACAAAATAT+TGG	-	chr2_1:72720776-72720795	None:intergenic	35.0%
	AGCGGTGAAATTGGGAATTA+GGG	-	chr2_1:72716853-72716872	None:intergenic	40.0%
	GATTGGGTAACTACGAGAAA+CGG	-	chr2_1:72716880-72716899	None:intergenic	40.0%
	TGGCGAATTTGAAAGGAGAA+AGG	-	chr2_1:72717066-72717085	None:intergenic	40.0%
	ATTGTGAGCAGAGGATAACT+TGG	-	chr2_1:72717609-72717628	None:intergenic	40.0%
	CTCAGATAAATTGTGAGCAG+AGG	-	chr2_1:72717618-72717637	None:intergenic	40.0%
	GAGAGAGTGAGATCCATTTA+AGG	+	chr2_1:72717635-72717654	Msa0223170:intron	40.0%
	GAGAGTGAGATCCATTTAAG+GGG	+	chr2_1:72717637-72717656	Msa0223170:intron	40.0%
	GAATGCTTAATCAAGGACAG+AGG	-	chr2_1:72717809-72717828	None:intergenic	40.0%
!!	TTTTGGGCTTTAATGCGACA+TGG	+	chr2_1:72717892-72717911	Msa0223170:intron	40.0%
	GCTGGTGGTTTATTTAACAG+TGG	+	chr2_1:72717917-72717936	Msa0223170:intron	40.0%
	ACTGCAATCAAACAAGGAGT+AGG	-	chr2_1:72718028-72718047	None:intergenic	40.0%
	AACTGCACTGCAATCAAACA+AGG	-	chr2_1:72718034-72718053	None:intergenic	40.0%
!	GCAGTGCAGTTTCTTCTTTT+TGG	+	chr2_1:72718043-72718062	Msa0223170:intron	40.0%
	CAGCTAAGCATAGCAATTAC+TGG	-	chr2_1:72718103-72718122	None:intergenic	40.0%
!	AGTTGATCCTATTTGGGGTT+GGG	-	chr2_1:72718296-72718315	None:intergenic	40.0%
!	AAGTTGATCCTATTTGGGGT+TGG	-	chr2_1:72718297-72718316	None:intergenic	40.0%
	AACCCCAAATAGGATCAACT+TGG	+	chr2_1:72718296-72718315	Msa0223170:intron	40.0%
!!	CTCCAAGTTGATCCTATTTG+GGG	-	chr2_1:72718301-72718320	None:intergenic	40.0%
	CATTGGATTCGACAAGCAAT+GGG	+	chr2_1:72718329-72718348	Msa0223170:CDS	40.0%
	GCCTCGTATTTGGATTACTT+TGG	+	chr2_1:72718351-72718370	Msa0223170:CDS	40.0%
	CCCAAAGTAATCCAAATACG+AGG	-	chr2_1:72718355-72718374	None:intergenic	40.0%
!	TTTTGTCGTAAGTCTGGGTT+GGG	-	chr2_1:72719283-72719302	None:intergenic	40.0%
	GATCATCACATACAAGTGTG+AGG	+	chr2_1:72719382-72719401	Msa0223170:intron	40.0%
	AAATTAAGTTAAGGGGCAGC+AGG	-	chr2_1:72719429-72719448	None:intergenic	40.0%
	TTGGTCCTTCTATCCATGTT+TGG	+	chr2_1:72719496-72719515	Msa0223170:intron	40.0%
	GATGAAGGACCAAAATGATG+TGG	+	chr2_1:72719640-72719659	Msa0223170:intron	40.0%
	GTGGTCCCAAACTTAATTTC+AGG	-	chr2_1:72719691-72719710	None:intergenic	40.0%
	ACTAGGCAAAATTACTTCCG+TGG	-	chr2_1:72719710-72719729	None:intergenic	40.0%
	ACCTGGGAATTGTATGAGTT+TGG	+	chr2_1:72719785-72719804	Msa0223170:intron	40.0%
	ACCAAACTCATACAATTCCC+AGG	-	chr2_1:72719789-72719808	None:intergenic	40.0%
!!!	GCTTTTTAGTTTTGTCCTCC+GGG	+	chr2_1:72719910-72719929	Msa0223170:intron	40.0%
!	GTTGATGAAGAGGAATTTGG+CGG	+	chr2_1:72719966-72719985	Msa0223170:CDS	40.0%
!	CAGTTTAGGACACTTCTGAT+CGG	+	chr2_1:72720383-72720402	Msa0223170:exon	40.0%
	ATCAATGAGAAAATGCGGGT+TGG	+	chr2_1:72720488-72720507	Msa0223170:three_prime_UTR	40.0%
	TTAATGACACCACACGTTTG+TGG	+	chr2_1:72720595-72720614	Msa0223170:three_prime_UTR	40.0%
!	CATAATTTGCCACAAACGTG+TGG	-	chr2_1:72720607-72720626	None:intergenic	40.0%
	CACACGTTTGTGGCAAATTA+TGG	+	chr2_1:72720605-72720624	Msa0223170:three_prime_UTR	40.0%
	GAGCGGTGAAATTGGGAATT+AGG	-	chr2_1:72716854-72716873	None:intergenic	45.0%
	AAGGAGAAAGGTGACCATTC+TGG	-	chr2_1:72717054-72717073	None:intergenic	45.0%
	AACCTTGTCCCAAGAAGCTT+CGG	-	chr2_1:72717101-72717120	None:intergenic	45.0%
	GGAGAAGTCAACCCCTTAAA+TGG	-	chr2_1:72717651-72717670	None:intergenic	45.0%
	TCTTAAGTATTCGGTCTGGC+CGG	-	chr2_1:72717699-72717718	None:intergenic	45.0%
	GGTCTCTTAAGTATTCGGTC+TGG	-	chr2_1:72717703-72717722	None:intergenic	45.0%
	GGCACAAGTGCTAGATACTT+GGG	-	chr2_1:72717724-72717743	None:intergenic	45.0%
	TGGCACAAGTGCTAGATACT+TGG	-	chr2_1:72717725-72717744	None:intergenic	45.0%
	GCACTTGTGCCAAACTATGT+TGG	+	chr2_1:72717733-72717752	Msa0223170:intron	45.0%
	CTTTAATGCGACATGGTTGC+TGG	+	chr2_1:72717899-72717918	Msa0223170:intron	45.0%
!	GTTGATCCTATTTGGGGTTG+GGG	-	chr2_1:72718295-72718314	None:intergenic	45.0%
	GCATTGGATTCGACAAGCAA+TGG	+	chr2_1:72718328-72718347	Msa0223170:CDS	45.0%
	AAATCCTCCAACCACTGGAA+AGG	-	chr2_1:72718381-72718400	None:intergenic	45.0%
!	TTCCAGTGGTTGGAGGATTT+TGG	+	chr2_1:72718381-72718400	Msa0223170:CDS	45.0%
	AACCAAAATCCTCCAACCAC+TGG	-	chr2_1:72718386-72718405	None:intergenic	45.0%
	TCACTCTATCCATAAGCCAC+TGG	+	chr2_1:72718462-72718481	Msa0223170:intron	45.0%
	TAAATCATCAGGCAAGCCAG+TGG	-	chr2_1:72718481-72718500	None:intergenic	45.0%
!	AAGCCTTATCCCACTAAGTG+GGG	-	chr2_1:72718997-72719016	None:intergenic	45.0%
!	CAAGCCTTATCCCACTAAGT+GGG	-	chr2_1:72718998-72719017	None:intergenic	45.0%
!	GTTTTGTCGTAAGTCTGGGT+TGG	-	chr2_1:72719284-72719303	None:intergenic	45.0%
	CTTAAGACTGAGAGCTCACT+GGG	-	chr2_1:72719558-72719577	None:intergenic	45.0%
	ACTTAAGACTGAGAGCTCAC+TGG	-	chr2_1:72719559-72719578	None:intergenic	45.0%
	ATACAATTCCCAGGTGCTTG+TGG	-	chr2_1:72719780-72719799	None:intergenic	45.0%
	TTGTCCTCCGGGATCATATA+TGG	+	chr2_1:72719921-72719940	Msa0223170:CDS	45.0%
	GTAACCATATATGATCCCGG+AGG	-	chr2_1:72719928-72719947	None:intergenic	45.0%
	TAGATGCAAAAAGCCCCTCT+TGG	-	chr2_1:72720007-72720026	None:intergenic	45.0%
!	TTTAGGACACTTCTGATCGG+CGG	+	chr2_1:72720386-72720405	Msa0223170:exon	45.0%
!!	AATGAGAAAATGCGGGTTGG+CGG	+	chr2_1:72720491-72720510	Msa0223170:three_prime_UTR	45.0%
!	CATTCTGATTTTGTCGAGGG+TGG	+	chr2_1:72720536-72720555	Msa0223170:three_prime_UTR	45.0%
	TGCTTGCAGGCAAATTCTGA+AGG	+	chr2_1:72720683-72720702	Msa0223170:three_prime_UTR	45.0%
!!	TATAAGATAAAATAAATAAT+GGG	-	chr2_1:72716949-72716968	None:intergenic	5.0%
!!	TTATAAGATAAAATAAATAA+TGG	-	chr2_1:72716950-72716969	None:intergenic	5.0%
	ACGGCAAGAGCGGTGAAATT+GGG	-	chr2_1:72716861-72716880	None:intergenic	50.0%
	AACGGCAAGAGCGGTGAAAT+TGG	-	chr2_1:72716862-72716881	None:intergenic	50.0%
	ACTACGAGAAACGGCAAGAG+CGG	-	chr2_1:72716871-72716890	None:intergenic	50.0%
!!	CCATTCTGGTGGTGTTGTTG+AGG	-	chr2_1:72717040-72717059	None:intergenic	50.0%
	CCTCAACAACACCACCAGAA+TGG	+	chr2_1:72717037-72717056	Msa0223170:CDS	50.0%
	GAGAAAGGTGACCATTCTGG+TGG	-	chr2_1:72717051-72717070	None:intergenic	50.0%
	AGCTTCGGGATCGAGCAATT+TGG	-	chr2_1:72717086-72717105	None:intergenic	50.0%
	ACCTTGTCCCAAGAAGCTTC+GGG	-	chr2_1:72717100-72717119	None:intergenic	50.0%
	TAATGCGACATGGTTGCTGG+TGG	+	chr2_1:72717902-72717921	Msa0223170:intron	50.0%
	CAAGCAATGGGCCTCGTATT+TGG	+	chr2_1:72718341-72718360	Msa0223170:CDS	50.0%
	CTTATCCCACTAAGTGGGGT+CGG	-	chr2_1:72718993-72719012	None:intergenic	50.0%
!	CCAAGCCTTATCCCACTAAG+TGG	-	chr2_1:72718999-72719018	None:intergenic	50.0%
	CCACTTAGTGGGATAAGGCT+TGG	+	chr2_1:72718996-72719015	Msa0223170:intron	50.0%
	TGAAGGTGAGGATTCCCTTC+TGG	-	chr2_1:72719248-72719267	None:intergenic	50.0%
	ACCACAAATCCACAAGCACC+TGG	+	chr2_1:72719768-72719787	Msa0223170:intron	50.0%
	CCACAAATCCACAAGCACCT+GGG	+	chr2_1:72719769-72719788	Msa0223170:intron	50.0%
	AAAAAGCCCCTCTTGGAGGA+GGG	-	chr2_1:72720000-72720019	None:intergenic	50.0%
	ATGCAAAAAGCCCCTCTTGG+AGG	-	chr2_1:72720004-72720023	None:intergenic	50.0%
	TCTAATCTGCGCAGCTTTCC+TGG	+	chr2_1:72720143-72720162	Msa0223170:intron	50.0%
	TCTAATCTGCGCAGCTTTCC+TGG	+	chr2_1:72720143-72720162	Msa0223170:intron	50.0%
	TCTAATCTGCGCAGCTTTCC+TGG	+	chr2_1:72720143-72720162	Msa0223170:intron	50.0%
	TTAGGACACTTCTGATCGGC+GGG	+	chr2_1:72720387-72720406	Msa0223170:exon	50.0%
!!	ATGAGAAAATGCGGGTTGGC+GGG	+	chr2_1:72720492-72720511	Msa0223170:three_prime_UTR	50.0%
	TGCTCGATCCCGAAGCTTCT+TGG	+	chr2_1:72717089-72717108	Msa0223170:CDS	55.0%
	GCTCGATCCCGAAGCTTCTT+GGG	+	chr2_1:72717090-72717109	Msa0223170:CDS	55.0%
	TCCCGAAGCTTCTTGGGACA+AGG	+	chr2_1:72717096-72717115	Msa0223170:CDS	55.0%
	TCACCTCCCCAACCCCAAAT+AGG	+	chr2_1:72718286-72718305	Msa0223170:intron	55.0%
!	GATCCTATTTGGGGTTGGGG+AGG	-	chr2_1:72718292-72718311	None:intergenic	55.0%
	TCAGGCAAGCCAGTGGCTTA+TGG	-	chr2_1:72718474-72718493	None:intergenic	55.0%
	ACTAAGTGGGGTCGGTTGCA+TGG	-	chr2_1:72718985-72719004	None:intergenic	55.0%
	ATGCAACCGACCCCACTTAG+TGG	+	chr2_1:72718984-72719003	Msa0223170:intron	55.0%
	TGCAACCGACCCCACTTAGT+GGG	+	chr2_1:72718985-72719004	Msa0223170:intron	55.0%
	CGACCCCACTTAGTGGGATA+AGG	+	chr2_1:72718991-72719010	Msa0223170:intron	55.0%
	CCCAGGTGCTTGTGGATTTG+TGG	-	chr2_1:72719772-72719791	None:intergenic	55.0%
	GAAGAGGAATTTGGCGGCCA+TGG	+	chr2_1:72719972-72719991	Msa0223170:CDS	55.0%
	CAAAAAGCCCCTCTTGGAGG+AGG	-	chr2_1:72720001-72720020	None:intergenic	55.0%
	TAGGACACTTCTGATCGGCG+GGG	+	chr2_1:72720388-72720407	Msa0223170:exon	55.0%
!!	ACTTTGGGGCGCCTTTCCAG+TGG	+	chr2_1:72718367-72718386	Msa0223170:CDS	60.0%
	CATGGAGCCCTCCTCCAAGA+GGG	+	chr2_1:72719990-72720009	Msa0223170:CDS	60.0%
	ATGGAGCCCTCCTCCAAGAG+GGG	+	chr2_1:72719991-72720010	Msa0223170:CDS	60.0%
	TTCGGTCTGGCCGGTGCGTA+CGG	-	chr2_1:72717690-72717709	None:intergenic	65.0%
!	TGGGGCGCCTTTCCAGTGGT+TGG	+	chr2_1:72718371-72718390	Msa0223170:CDS	65.0%
	GGCGCCTTTCCAGTGGTTGG+AGG	+	chr2_1:72718374-72718393	Msa0223170:CDS	65.0%
	CCTCTTGGAGGAGGGCTCCA+TGG	-	chr2_1:72719992-72720011	None:intergenic	65.0%
	CCATGGAGCCCTCCTCCAAG+AGG	+	chr2_1:72719989-72720008	Msa0223170:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	72716692	72720918	72716692	ID=Msa0223170;Name=Msa0223170
chr2_1	mRNA	72716692	72720918	72716692	ID=Msa0223170-mRNA-1;Parent=Msa0223170;Name=Msa0223170-mRNA-1;_AED=0.05;_eAED=0.05;_QI=324\|1\|1\|1\|0.66\|0.75\|4\|517\|124
chr2_1	exon	72716692	72717117	72716692	ID=Msa0223170-mRNA-1:exon:21045;Parent=Msa0223170-mRNA-1
chr2_1	exon	72718308	72718411	72718308	ID=Msa0223170-mRNA-1:exon:21046;Parent=Msa0223170-mRNA-1
chr2_1	exon	72719911	72720040	72719911	ID=Msa0223170-mRNA-1:exon:21047;Parent=Msa0223170-mRNA-1
chr2_1	exon	72720363	72720918	72720363	ID=Msa0223170-mRNA-1:exon:21048;Parent=Msa0223170-mRNA-1
chr2_1	five_prime_UTR	72716692	72717015	72716692	ID=Msa0223170-mRNA-1:five_prime_utr;Parent=Msa0223170-mRNA-1
chr2_1	CDS	72717016	72717117	72717016	ID=Msa0223170-mRNA-1:cds;Parent=Msa0223170-mRNA-1
chr2_1	CDS	72718308	72718411	72718308	ID=Msa0223170-mRNA-1:cds;Parent=Msa0223170-mRNA-1
chr2_1	CDS	72719911	72720040	72719911	ID=Msa0223170-mRNA-1:cds;Parent=Msa0223170-mRNA-1
chr2_1	CDS	72720363	72720401	72720363	ID=Msa0223170-mRNA-1:cds;Parent=Msa0223170-mRNA-1
chr2_1	three_prime_UTR	72720402	72720918	72720402	ID=Msa0223170-mRNA-1:three_prime_utr;Parent=Msa0223170-mRNA-1

Gene Sequence	>Msa0223170 ATGAAAGAAGCTAAATCTGTTCCTCAACAACACCACCAGAATGGTCACCTTTCTCCTTTCAAATTCGCCAAATTGCTCGATCCCGAAGCTTCTTGGGACAAGGATCAACTTGGAGATGTATTGCATTGGATTCGACAAGCAATGGGCCTCGTATTTGGATTACTTTGGGGCGCCTTTCCAGTGGTTGGAGGATTTTGGTTTATCCTCTTTTTAGTTTTGTCCTCCGGGATCATATATGGTTACTATTCAGTAATATTGAAGGTTGATGAAGAGGAATTTGGCGGCCATGGAGCCCTCCTCCAAGAGGGGCTTTTTGCATCTACAACTCTTTTTCTGCTTTCCTGGATTCTAGTCTACAGTTTAGGACACTTCTGA
Protein sequence	>Msa0223170 MKEAKSVPQQHHQNGHLSPFKFAKLLDPEASWDKDQLGDVLHWIRQAMGLVFGLLWGAFPVVGGFWFILFLVLSSGIIYGYYSVILKVDEEEFGGHGALLQEGLFASTTLFLLSWILVYSLGHF*