AlfalfaGEDB | Msa0277510

M. sativa cultivar zhongmu-4 / Msa0277510

KEGG: K08145
GO: GO:0000041 GO:0000325 GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0009507 GO:0009536 GO:0009705 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0034219 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0070838 GO:0071702 GO:0072511 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1902600 GO:1904659
PFAM: PF00083.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277510	KEH36721.1	89.552	67	7	0	15	81	330	396	6.27e-32	124

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277510	sp\|Q8LBI9\|EDL16_ARATH	68.657	67	21	0	13	79	412	478	4.04e-24	97.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277510	A0A072V4W8	89.552	67	7	0	15	81	330	396	3.00e-32	124

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0142560	Msa0277510	0.802310	5.960806e-49	-8.615850e-47
Msa0277510	Msa0277520	0.837343	5.496008e-57	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277510	MtrunA17_Chr2g0286721	89.552	67	7	15	81	419	485	1.73e-35	124
Msa0277510	MtrunA17_Chr4g0066581	83.582	67	11	15	81	422	488	1.22e-33	119
Msa0277510	MtrunA17_Chr7g0259221	61.194	67	26	15	81	411	477	9.91e-22	87.0
Msa0277510	MtrunA17_Chr7g0273261	60.606	66	26	15	80	173	238	1.71e-21	84.3
Msa0277510	MtrunA17_Chr7g0273281	58.209	67	28	15	81	431	497	5.95e-20	82.0
Msa0277510	MtrunA17_Chr7g0259471	55.224	67	30	15	81	409	475	1.73e-19	80.9
Msa0277510	MtrunA17_Chr5g0404181	48.438	64	33	13	76	65	128	3.53e-14	62.8
Msa0277510	MtrunA17_Chr8g0373181	48.438	64	33	13	76	419	482	1.70e-13	63.5
Msa0277510	MtrunA17_Chr1g0155221	49.206	63	32	14	76	423	485	1.07e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277510	AT5G18840.1	68.657	67	21	13	79	412	478	4.11e-25	97.1
Msa0277510	AT2G48020.2	57.353	68	29	13	80	395	462	5.95e-21	85.1
Msa0277510	AT2G48020.1	57.353	68	29	13	80	395	462	5.95e-21	85.1
Msa0277510	AT3G05150.1	61.404	57	22	21	77	411	467	2.84e-20	83.2
Msa0277510	AT3G05150.3	61.404	57	22	21	77	411	467	2.84e-20	83.2
Msa0277510	AT3G05150.2	61.404	57	22	21	77	411	467	2.99e-20	83.2
Msa0277510	AT1G54730.5	53.030	66	31	15	80	380	445	2.71e-19	80.5
Msa0277510	AT1G54730.2	53.030	66	31	15	80	401	466	2.76e-19	80.5
Msa0277510	AT3G20460.1	58.621	58	24	23	80	430	487	5.65e-17	73.9
Msa0277510	AT1G08900.3	56.604	53	23	24	76	395	447	1.02e-16	73.2
Msa0277510	AT1G08900.5	56.604	53	23	24	76	361	413	1.05e-16	73.2
Msa0277510	AT1G08900.1	56.604	53	23	24	76	403	455	1.07e-16	73.2
Msa0277510	AT1G08900.2	56.604	53	23	24	76	403	455	1.07e-16	73.2
Msa0277510	AT1G08890.1	54.717	53	24	24	76	405	457	5.71e-16	71.2
Msa0277510	AT4G04750.1	52.727	55	26	27	81	419	473	2.03e-14	66.6
Msa0277510	AT4G04750.2	52.727	55	26	27	81	419	473	2.03e-14	66.6
Msa0277510	AT4G04750.3	52.727	55	26	27	81	463	517	2.05e-14	66.6
Msa0277510	AT4G04760.3	50.909	55	27	27	81	356	410	2.40e-14	66.6
Msa0277510	AT4G04760.2	50.909	55	27	27	81	412	466	2.82e-14	66.2
Msa0277510	AT4G04760.1	54.902	51	23	27	77	415	465	3.15e-14	66.2
Msa0277510	AT3G05160.3	43.284	67	38	15	81	341	407	7.00e-14	65.1
Msa0277510	AT3G05160.2	43.284	67	38	15	81	341	407	7.00e-14	65.1
Msa0277510	AT3G05160.1	43.284	67	38	15	81	390	456	8.56e-14	65.1
Msa0277510	AT1G19450.1	52.308	65	31	13	77	421	485	9.14e-14	64.7
Msa0277510	AT1G08930.1	44.828	58	32	24	81	436	493	1.12e-13	64.7
Msa0277510	AT1G08930.2	44.828	58	32	24	81	436	493	1.12e-13	64.7
Msa0277510	AT3G05165.3	42.373	59	34	23	81	407	465	1.29e-13	64.3
Msa0277510	AT3G05165.2	42.373	59	34	23	81	407	465	1.29e-13	64.3
Msa0277510	AT3G05165.1	42.373	59	34	23	81	407	465	1.29e-13	64.3
Msa0277510	AT5G27360.1	44.615	65	36	15	79	404	468	1.23e-12	61.6
Msa0277510	AT5G27360.3	46.032	63	34	15	77	452	514	1.24e-12	61.6
Msa0277510	AT5G27360.2	44.615	65	36	15	79	300	364	1.35e-12	61.6
Msa0277510	AT5G27360.5	44.615	65	36	15	79	300	364	1.35e-12	61.6
Msa0277510	AT3G05155.1	47.619	63	33	14	76	393	455	3.93e-12	60.1
Msa0277510	AT5G27350.1	55.102	49	22	29	77	414	462	5.60e-12	59.7
Msa0277510	AT1G75220.1	46.154	65	35	13	77	420	484	7.03e-12	59.7
Msa0277510	AT1G08920.2	39.655	58	35	24	81	417	474	6.25e-11	57.0
Msa0277510	AT1G08920.1	39.655	58	35	24	81	410	467	6.88e-11	56.6

Find 26 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTAAATATATCAAAATAT+TGG	0.220828	2_3:+10092114	None:intergenic
CAAAACATTGGAAGAAATTC+AGG	0.233434	2_3:-10092230	Msa0277510:CDS
TGATAACTAATGCCGATAAT+TGG	0.240590	2_3:+10092054	None:intergenic
CATTAGTTATCAAATTCTTT+TGG	0.260068	2_3:-10092043	Msa0277510:three_prime_UTR
TTTGAACCAAGATGTCATTA+AGG	0.306072	2_3:-10091975	Msa0277510:three_prime_UTR
TGGATCGGTTATATACATTA+TGG	0.339834	2_3:-10092163	Msa0277510:three_prime_UTR
CAAAGCATAGATAGAATAAC+AGG	0.349701	2_3:-10092004	Msa0277510:three_prime_UTR
ATTAAATATATCAAAATATT+GGG	0.353502	2_3:+10092115	None:intergenic
GACATTGCTTCTGTCTCTCT+TGG	0.367254	2_3:-10092141	Msa0277510:three_prime_UTR
CCTGGACACTGTCCAATTAT+CGG	0.407891	2_3:-10092066	Msa0277510:three_prime_UTR
CTACAAATAGAATGGTTAAG+AGG	0.421225	2_3:+10092278	None:intergenic
CTTATGAGTTGGAGTTCTCC+TGG	0.439978	2_3:-10092428	Msa0277510:intron
TCCAGGCCTTAATGACATCT+TGG	0.442306	2_3:+10091969	None:intergenic
CCATAGCGTGGCTAGGAGCT+TGG	0.470850	2_3:-10092475	Msa0277510:CDS
TATGCTTTGTCTGTCCTGAA+TGG	0.495388	2_3:+10092018	None:intergenic
CCGATAATTGGACAGTGTCC+AGG	0.503983	2_3:+10092066	None:intergenic
TTAAGTAAATTACAACATCC+AGG	0.527916	2_3:+10091952	None:intergenic
ACCAAGATGTCATTAAGGCC+TGG	0.537644	2_3:-10091970	Msa0277510:three_prime_UTR
TACAAATAGAATGGTTAAGA+GGG	0.541695	2_3:+10092279	None:intergenic
TGGTGACCTGATACATGAAC+AGG	0.553998	2_3:-10093173	None:intergenic
CCAAGCTCCTAGCCACGCTA+TGG	0.563149	2_3:+10092475	None:intergenic
TCAGAAACCATAGCGTGGCT+AGG	0.575426	2_3:-10092482	Msa0277510:CDS
TGAAACCAAAGGCAAAACAT+TGG	0.583752	2_3:-10092242	Msa0277510:CDS
ATCAAGCTATAAAGCAGACC+TGG	0.605933	2_3:-10092084	Msa0277510:three_prime_UTR
AAAGTAGTCCCTGAAACCAA+AGG	0.635083	2_3:-10092253	Msa0277510:CDS
CTGCTTCAGAAACCATAGCG+TGG	0.697033	2_3:-10092487	Msa0277510:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GCAATATATATATATATATA+TGG	-	chr2_3:10092908-10092927	Msa0277510:intron	10.0%
!!	ATATATATATATATATGGAT+CGG	-	chr2_3:10092913-10092932	Msa0277510:intron	10.0%
!!	CATTAAATATATCAAAATAT+TGG	+	chr2_3:10092980-10092999	None:intergenic	10.0%
!!	GCAATATATATATATATATA+TGG	-	chr2_3:10092908-10092927	Msa0277510:intron	10.0%
!!	ATATATATATATATATGGAT+CGG	-	chr2_3:10092913-10092932	Msa0277510:intron	10.0%
!!	CATTAAATATATCAAAATAT+TGG	+	chr2_3:10092980-10092999	None:intergenic	10.0%
!!!	ATTTATTTTGTAATTGAATG+TGG	+	chr2_3:10091959-10091978	None:intergenic	15.0%
!!!	TAATAATTGTATTGTTTTTG+TGG	-	chr2_3:10092758-10092777	Msa0277510:intron	15.0%
!!!	ATTTATTTTGTAATTGAATG+TGG	+	chr2_3:10091959-10091978	None:intergenic	15.0%
!!!	TAATAATTGTATTGTTTTTG+TGG	-	chr2_3:10092758-10092777	Msa0277510:intron	15.0%
!!	TAATTCAGTAATGAATCAAT+AGG	-	chr2_3:10091989-10092008	Msa0277510:three_prime_UTR	20.0%
!!!	TTTTATTGTCTGTTTATGTT+TGG	+	chr2_3:10092150-10092169	None:intergenic	20.0%
!!	TTGTTAAGGATATAATAGTT+TGG	+	chr2_3:10092245-10092264	None:intergenic	20.0%
!!!	ACATGTTTTTGTTTTTGTTA+AGG	+	chr2_3:10092259-10092278	None:intergenic	20.0%
!!	CATGATAAAGAACTTTAATT+TGG	+	chr2_3:10092425-10092444	None:intergenic	20.0%
!!	TCAAGTGTTAAAGTAAAATT+GGG	+	chr2_3:10092503-10092522	None:intergenic	20.0%
!!	TTCAAGTGTTAAAGTAAAAT+TGG	+	chr2_3:10092504-10092523	None:intergenic	20.0%
!!!	CATTAGTTATCAAATTCTTT+TGG	-	chr2_3:10093048-10093067	Msa0277510:intron	20.0%
!!	TAATTCAGTAATGAATCAAT+AGG	-	chr2_3:10091989-10092008	Msa0277510:three_prime_UTR	20.0%
!!!	TTTTATTGTCTGTTTATGTT+TGG	+	chr2_3:10092150-10092169	None:intergenic	20.0%
!!	TTGTTAAGGATATAATAGTT+TGG	+	chr2_3:10092245-10092264	None:intergenic	20.0%
!!!	ACATGTTTTTGTTTTTGTTA+AGG	+	chr2_3:10092259-10092278	None:intergenic	20.0%
!!	CATGATAAAGAACTTTAATT+TGG	+	chr2_3:10092425-10092444	None:intergenic	20.0%
!!	TCAAGTGTTAAAGTAAAATT+GGG	+	chr2_3:10092503-10092522	None:intergenic	20.0%
!!	TTCAAGTGTTAAAGTAAAAT+TGG	+	chr2_3:10092504-10092523	None:intergenic	20.0%
!!!	CATTAGTTATCAAATTCTTT+TGG	-	chr2_3:10093048-10093067	Msa0277510:intron	20.0%
!	TCTCACAAAAAGAATTATCA+GGG	+	chr2_3:10092045-10092064	None:intergenic	25.0%
!	ATCTCACAAAAAGAATTATC+AGG	+	chr2_3:10092046-10092065	None:intergenic	25.0%
!!	TGATAATTCTTTTTGTGAGA+TGG	-	chr2_3:10092045-10092064	Msa0277510:three_prime_UTR	25.0%
!	TCAAATGATCAATTGTCAAA+AGG	-	chr2_3:10092119-10092138	Msa0277510:three_prime_UTR	25.0%
!	AACAAAAACAAAAACATGTC+TGG	-	chr2_3:10092260-10092279	Msa0277510:CDS	25.0%
!!!	ATTTTGATGATGCATTAAGT+TGG	+	chr2_3:10092301-10092320	None:intergenic	25.0%
!!!	GATAAAGAACTTTAATTTGG+TGG	+	chr2_3:10092422-10092441	None:intergenic	25.0%
!!!	GTAAGTGTAGATTTTATTTG+AGG	+	chr2_3:10092450-10092469	None:intergenic	25.0%
!!	CTTTCAATTTTCTTATGAGT+TGG	-	chr2_3:10092652-10092671	Msa0277510:intron	25.0%
!	TACAAATAGAATGGTTAAGA+GGG	+	chr2_3:10092815-10092834	None:intergenic	25.0%
!!	TACTTTTGCTACAAATAGAA+TGG	+	chr2_3:10092824-10092843	None:intergenic	25.0%
!	TTAAGTAAATTACAACATCC+AGG	+	chr2_3:10093142-10093161	None:intergenic	25.0%
!	TCTCACAAAAAGAATTATCA+GGG	+	chr2_3:10092045-10092064	None:intergenic	25.0%
!	ATCTCACAAAAAGAATTATC+AGG	+	chr2_3:10092046-10092065	None:intergenic	25.0%
!!	TGATAATTCTTTTTGTGAGA+TGG	-	chr2_3:10092045-10092064	Msa0277510:three_prime_UTR	25.0%
!	TCAAATGATCAATTGTCAAA+AGG	-	chr2_3:10092119-10092138	Msa0277510:three_prime_UTR	25.0%
!	AACAAAAACAAAAACATGTC+TGG	-	chr2_3:10092260-10092279	Msa0277510:CDS	25.0%
!!!	ATTTTGATGATGCATTAAGT+TGG	+	chr2_3:10092301-10092320	None:intergenic	25.0%
!!!	GATAAAGAACTTTAATTTGG+TGG	+	chr2_3:10092422-10092441	None:intergenic	25.0%
!!!	GTAAGTGTAGATTTTATTTG+AGG	+	chr2_3:10092450-10092469	None:intergenic	25.0%
!!	CTTTCAATTTTCTTATGAGT+TGG	-	chr2_3:10092652-10092671	Msa0277510:intron	25.0%
!	TACAAATAGAATGGTTAAGA+GGG	+	chr2_3:10092815-10092834	None:intergenic	25.0%
!!	TACTTTTGCTACAAATAGAA+TGG	+	chr2_3:10092824-10092843	None:intergenic	25.0%
!	TTAAGTAAATTACAACATCC+AGG	+	chr2_3:10093142-10093161	None:intergenic	25.0%
	TCACAAAAAGAATTATCAGG+GGG	+	chr2_3:10092043-10092062	None:intergenic	30.0%
	CTCACAAAAAGAATTATCAG+GGG	+	chr2_3:10092044-10092063	None:intergenic	30.0%
!	TAATTCTTTTTGTGAGATGG+AGG	-	chr2_3:10092048-10092067	Msa0277510:three_prime_UTR	30.0%
!	AATTCTTTTTGTGAGATGGA+GGG	-	chr2_3:10092049-10092068	Msa0277510:three_prime_UTR	30.0%
	ATACAGTTCACTTCAACTTA+AGG	-	chr2_3:10092353-10092372	Msa0277510:intron	30.0%
!!	AATCTCACTTGTTATCGATT+AGG	+	chr2_3:10092546-10092565	None:intergenic	30.0%
	TGATTATGTGCTTAATTACC+AGG	+	chr2_3:10092684-10092703	None:intergenic	30.0%
!!!	ATTGTATTGTTTTTGTGGAC+AGG	-	chr2_3:10092763-10092782	Msa0277510:intron	30.0%
	CTACAAATAGAATGGTTAAG+AGG	+	chr2_3:10092816-10092835	None:intergenic	30.0%
	CAAAACATTGGAAGAAATTC+AGG	-	chr2_3:10092861-10092880	Msa0277510:intron	30.0%
	TGGATCGGTTATATACATTA+TGG	-	chr2_3:10092928-10092947	Msa0277510:intron	30.0%
	TGATAACTAATGCCGATAAT+TGG	+	chr2_3:10093040-10093059	None:intergenic	30.0%
!	AAATTCTTTTGGATCCATTC+AGG	-	chr2_3:10093059-10093078	Msa0277510:intron	30.0%
	CAAAGCATAGATAGAATAAC+AGG	-	chr2_3:10093087-10093106	Msa0277510:intron	30.0%
	TTTGAACCAAGATGTCATTA+AGG	-	chr2_3:10093116-10093135	Msa0277510:intron	30.0%
	TCACAAAAAGAATTATCAGG+GGG	+	chr2_3:10092043-10092062	None:intergenic	30.0%
	CTCACAAAAAGAATTATCAG+GGG	+	chr2_3:10092044-10092063	None:intergenic	30.0%
!	TAATTCTTTTTGTGAGATGG+AGG	-	chr2_3:10092048-10092067	Msa0277510:three_prime_UTR	30.0%
!	AATTCTTTTTGTGAGATGGA+GGG	-	chr2_3:10092049-10092068	Msa0277510:three_prime_UTR	30.0%
	ATACAGTTCACTTCAACTTA+AGG	-	chr2_3:10092353-10092372	Msa0277510:intron	30.0%
!!	AATCTCACTTGTTATCGATT+AGG	+	chr2_3:10092546-10092565	None:intergenic	30.0%
	TGATTATGTGCTTAATTACC+AGG	+	chr2_3:10092684-10092703	None:intergenic	30.0%
!!!	ATTGTATTGTTTTTGTGGAC+AGG	-	chr2_3:10092763-10092782	Msa0277510:intron	30.0%
	CTACAAATAGAATGGTTAAG+AGG	+	chr2_3:10092816-10092835	None:intergenic	30.0%
	CAAAACATTGGAAGAAATTC+AGG	-	chr2_3:10092861-10092880	Msa0277510:intron	30.0%
	TGGATCGGTTATATACATTA+TGG	-	chr2_3:10092928-10092947	Msa0277510:intron	30.0%
	TGATAACTAATGCCGATAAT+TGG	+	chr2_3:10093040-10093059	None:intergenic	30.0%
!	AAATTCTTTTGGATCCATTC+AGG	-	chr2_3:10093059-10093078	Msa0277510:intron	30.0%
	CAAAGCATAGATAGAATAAC+AGG	-	chr2_3:10093087-10093106	Msa0277510:intron	30.0%
	TTTGAACCAAGATGTCATTA+AGG	-	chr2_3:10093116-10093135	Msa0277510:intron	30.0%
!	ATTCTTTTTGTGAGATGGAG+GGG	-	chr2_3:10092050-10092069	Msa0277510:three_prime_UTR	35.0%
	GAAGACGAGGATCTTTATAT+AGG	+	chr2_3:10092178-10092197	None:intergenic	35.0%
	ATAGTCAAACTAATCCCGTA+AGG	-	chr2_3:10092200-10092219	Msa0277510:exon	35.0%
!	TATAATAGTTTGGTTCCCTC+TGG	+	chr2_3:10092235-10092254	None:intergenic	35.0%
	TATGTCCATCCAGTTTAATG+TGG	+	chr2_3:10092336-10092355	None:intergenic	35.0%
!	GGGTTTAATGATTGATAGCA+TGG	+	chr2_3:10092483-10092502	None:intergenic	35.0%
!!	GGTACTATGTTTTTGTATGC+TGG	-	chr2_3:10092784-10092803	Msa0277510:intron	35.0%
!!	ATGTTTTGCCTTTGGTTTCA+GGG	+	chr2_3:10092849-10092868	None:intergenic	35.0%
!!	AATGTTTTGCCTTTGGTTTC+AGG	+	chr2_3:10092850-10092869	None:intergenic	35.0%
	TGAAACCAAAGGCAAAACAT+TGG	-	chr2_3:10092849-10092868	Msa0277510:intron	35.0%
!	TTCTTCCAATGTTTTGCCTT+TGG	+	chr2_3:10092857-10092876	None:intergenic	35.0%
!	ATTCTTTTTGTGAGATGGAG+GGG	-	chr2_3:10092050-10092069	Msa0277510:three_prime_UTR	35.0%
	GAAGACGAGGATCTTTATAT+AGG	+	chr2_3:10092178-10092197	None:intergenic	35.0%
	ATAGTCAAACTAATCCCGTA+AGG	-	chr2_3:10092200-10092219	Msa0277510:exon	35.0%
!	TATAATAGTTTGGTTCCCTC+TGG	+	chr2_3:10092235-10092254	None:intergenic	35.0%
	TATGTCCATCCAGTTTAATG+TGG	+	chr2_3:10092336-10092355	None:intergenic	35.0%
!	GGGTTTAATGATTGATAGCA+TGG	+	chr2_3:10092483-10092502	None:intergenic	35.0%
!!	GGTACTATGTTTTTGTATGC+TGG	-	chr2_3:10092784-10092803	Msa0277510:intron	35.0%
!!	ATGTTTTGCCTTTGGTTTCA+GGG	+	chr2_3:10092849-10092868	None:intergenic	35.0%
!!	AATGTTTTGCCTTTGGTTTC+AGG	+	chr2_3:10092850-10092869	None:intergenic	35.0%
	TGAAACCAAAGGCAAAACAT+TGG	-	chr2_3:10092849-10092868	Msa0277510:intron	35.0%
!	TTCTTCCAATGTTTTGCCTT+TGG	+	chr2_3:10092857-10092876	None:intergenic	35.0%
!	TTCTTTTTGTGAGATGGAGG+GGG	-	chr2_3:10092051-10092070	Msa0277510:three_prime_UTR	40.0%
	AGTTTGACTATCTGAAGACG+AGG	+	chr2_3:10092191-10092210	None:intergenic	40.0%
	ATGATGCCACCACATTAAAC+TGG	-	chr2_3:10092324-10092343	Msa0277510:intron	40.0%
	CTTCAACTTAAGGAAGCCAA+TGG	-	chr2_3:10092363-10092382	Msa0277510:intron	40.0%
	AGCCAATGGCTACATAATCA+AGG	-	chr2_3:10092377-10092396	Msa0277510:intron	40.0%
	CTCCTTGATTATGTAGCCAT+TGG	+	chr2_3:10092382-10092401	None:intergenic	40.0%
	AAAGTAGTCCCTGAAACCAA+AGG	-	chr2_3:10092838-10092857	Msa0277510:intron	40.0%
	ATCAAGCTATAAAGCAGACC+TGG	-	chr2_3:10093007-10093026	Msa0277510:intron	40.0%
	TATGCTTTGTCTGTCCTGAA+TGG	+	chr2_3:10093076-10093095	None:intergenic	40.0%
!	TTCTTTTTGTGAGATGGAGG+GGG	-	chr2_3:10092051-10092070	Msa0277510:three_prime_UTR	40.0%
	AGTTTGACTATCTGAAGACG+AGG	+	chr2_3:10092191-10092210	None:intergenic	40.0%
	ATGATGCCACCACATTAAAC+TGG	-	chr2_3:10092324-10092343	Msa0277510:intron	40.0%
	CTTCAACTTAAGGAAGCCAA+TGG	-	chr2_3:10092363-10092382	Msa0277510:intron	40.0%
	AGCCAATGGCTACATAATCA+AGG	-	chr2_3:10092377-10092396	Msa0277510:intron	40.0%
	CTCCTTGATTATGTAGCCAT+TGG	+	chr2_3:10092382-10092401	None:intergenic	40.0%
	AAAGTAGTCCCTGAAACCAA+AGG	-	chr2_3:10092838-10092857	Msa0277510:intron	40.0%
	ATCAAGCTATAAAGCAGACC+TGG	-	chr2_3:10093007-10093026	Msa0277510:intron	40.0%
	TATGCTTTGTCTGTCCTGAA+TGG	+	chr2_3:10093076-10093095	None:intergenic	40.0%
!!	TCTGGTGATGGATTCCTTAC+GGG	+	chr2_3:10092217-10092236	None:intergenic	45.0%
!!	CTCTGGTGATGGATTCCTTA+CGG	+	chr2_3:10092218-10092237	None:intergenic	45.0%
	GTAAGGAATCCATCACCAGA+GGG	-	chr2_3:10092217-10092236	Msa0277510:CDS	45.0%
	TGCCACCACATTAAACTGGA+TGG	-	chr2_3:10092328-10092347	Msa0277510:intron	45.0%
	GTCCATCCAGTTTAATGTGG+TGG	+	chr2_3:10092333-10092352	None:intergenic	45.0%
!!!	CGAAGCAGAACGTTTTGAAG+AGG	+	chr2_3:10092574-10092593	None:intergenic	45.0%
!	CTTATGAGTTGGAGTTCTCC+TGG	-	chr2_3:10092663-10092682	Msa0277510:intron	45.0%
	GACATTGCTTCTGTCTCTCT+TGG	-	chr2_3:10092950-10092969	Msa0277510:intron	45.0%
	CCTGGACACTGTCCAATTAT+CGG	-	chr2_3:10093025-10093044	Msa0277510:intron	45.0%
	ACCAAGATGTCATTAAGGCC+TGG	-	chr2_3:10093121-10093140	Msa0277510:intron	45.0%
	TCCAGGCCTTAATGACATCT+TGG	+	chr2_3:10093125-10093144	None:intergenic	45.0%
!!	TCTGGTGATGGATTCCTTAC+GGG	+	chr2_3:10092217-10092236	None:intergenic	45.0%
!!	CTCTGGTGATGGATTCCTTA+CGG	+	chr2_3:10092218-10092237	None:intergenic	45.0%
	GTAAGGAATCCATCACCAGA+GGG	-	chr2_3:10092217-10092236	Msa0277510:CDS	45.0%
	TGCCACCACATTAAACTGGA+TGG	-	chr2_3:10092328-10092347	Msa0277510:intron	45.0%
	GTCCATCCAGTTTAATGTGG+TGG	+	chr2_3:10092333-10092352	None:intergenic	45.0%
!!!	CGAAGCAGAACGTTTTGAAG+AGG	+	chr2_3:10092574-10092593	None:intergenic	45.0%
!	CTTATGAGTTGGAGTTCTCC+TGG	-	chr2_3:10092663-10092682	Msa0277510:intron	45.0%
	GACATTGCTTCTGTCTCTCT+TGG	-	chr2_3:10092950-10092969	Msa0277510:intron	45.0%
	CCTGGACACTGTCCAATTAT+CGG	-	chr2_3:10093025-10093044	Msa0277510:intron	45.0%
	ACCAAGATGTCATTAAGGCC+TGG	-	chr2_3:10093121-10093140	Msa0277510:intron	45.0%
	TCCAGGCCTTAATGACATCT+TGG	+	chr2_3:10093125-10093144	None:intergenic	45.0%
!!	ATTAAATATATCAAAATATT+GGG	+	chr2_3:10092979-10092998	None:intergenic	5.0%
!!	ATTAAATATATCAAAATATT+GGG	+	chr2_3:10092979-10092998	None:intergenic	5.0%
	CGTAAGGAATCCATCACCAG+AGG	-	chr2_3:10092216-10092235	Msa0277510:CDS	50.0%
!	AGTTTGGTTCCCTCTGGTGA+TGG	+	chr2_3:10092229-10092248	None:intergenic	50.0%
	CTGCTTCAGAAACCATAGCG+TGG	-	chr2_3:10092604-10092623	Msa0277510:intron	50.0%
	TCAGAAACCATAGCGTGGCT+AGG	-	chr2_3:10092609-10092628	Msa0277510:intron	50.0%
	CCGATAATTGGACAGTGTCC+AGG	+	chr2_3:10093028-10093047	None:intergenic	50.0%
	CGTAAGGAATCCATCACCAG+AGG	-	chr2_3:10092216-10092235	Msa0277510:CDS	50.0%
!	AGTTTGGTTCCCTCTGGTGA+TGG	+	chr2_3:10092229-10092248	None:intergenic	50.0%
	CTGCTTCAGAAACCATAGCG+TGG	-	chr2_3:10092604-10092623	Msa0277510:intron	50.0%
	TCAGAAACCATAGCGTGGCT+AGG	-	chr2_3:10092609-10092628	Msa0277510:intron	50.0%
	CCGATAATTGGACAGTGTCC+AGG	+	chr2_3:10093028-10093047	None:intergenic	50.0%
	CCAAGCTCCTAGCCACGCTA+TGG	+	chr2_3:10092619-10092638	None:intergenic	60.0%
	CCATAGCGTGGCTAGGAGCT+TGG	-	chr2_3:10092616-10092635	Msa0277510:intron	60.0%
	CCAAGCTCCTAGCCACGCTA+TGG	+	chr2_3:10092619-10092638	None:intergenic	60.0%
	CCATAGCGTGGCTAGGAGCT+TGG	-	chr2_3:10092616-10092635	Msa0277510:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	10091932	10093181	10091932	ID=Msa0277510;Name=Msa0277510
chr2_3	mRNA	10091932	10093181	10091932	ID=Msa0277510-mRNA-1;Parent=Msa0277510;Name=Msa0277510-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0\|0.5\|0.33\|0.66\|0\|0\|3\|281\|81
chr2_3	exon	10091932	10092328	10091932	ID=Msa0277510-mRNA-1:exon:4548;Parent=Msa0277510-mRNA-1
chr2_3	exon	10092429	10092550	10092429	ID=Msa0277510-mRNA-1:exon:4547;Parent=Msa0277510-mRNA-1
chr2_3	exon	10093174	10093181	10093174	ID=Msa0277510-mRNA-1:exon:4546;Parent=Msa0277510-mRNA-1
chr2_3	CDS	10093174	10093181	10093174	ID=Msa0277510-mRNA-1:cds;Parent=Msa0277510-mRNA-1
chr2_3	CDS	10092429	10092550	10092429	ID=Msa0277510-mRNA-1:cds;Parent=Msa0277510-mRNA-1
chr2_3	CDS	10092213	10092328	10092213	ID=Msa0277510-mRNA-1:cds;Parent=Msa0277510-mRNA-1
chr2_3	three_prime_UTR	10091932	10092212	10091932	ID=Msa0277510-mRNA-1:three_prime_utr;Parent=Msa0277510-mRNA-1

Gene Sequence	>Msa0277510 ATGAACAGATTAAATACCTCTTCAAAACGTTCTGCTTCGTTAGCTGATTCTGCTTCAGAAACCATAGCGTGGCTAGGAGCTTGGATAGTTTCATATACTTTCAATTTTCTTATGAGTTGGAGTTCTCCTGGTACTATGTTTTTGTATGCTGGATGTTCCCTCTTAACCATTCTATTTGTAGCAAAAGTAGTCCCTGAAACCAAAGGCAAAACATTGGAAGAAATTCAGGCTTGCATTAGTTCTTAG
Protein sequence	>Msa0277510 MNRLNTSSKRSASLADSASETIAWLGAWIVSYTFNFLMSWSSPGTMFLYAGCSLLTILFVAKVVPETKGKTLEEIQACISS*