AlfalfaGEDB | Msa0282490

M. sativa cultivar zhongmu-4 / Msa0282490

KEGG: K13025
GO: -
PFAM: PF00270.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282490	XP_013463348.1	98.058	412	2	1	1	412	1	406	0.0	829

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282490	sp\|Q5VNM3\|IF43A_ORYSJ	85.819	409	52	1	4	412	2	404	0.0	739

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282490	A0A072V6Z3	98.058	412	2	1	1	412	1	406	0.0	829

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0282490	Msa0719500	0.800006	1.764671e-48	-8.615850e-47
Msa0282490	Msa0931450	0.807095	5.974614e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282490	MtrunA17_Chr2g0297261	98.058	412	2	1	1	412	1	406	0.0	829
Msa0282490	MtrunA17_Chr2g0299681	64.908	379	127	1	34	412	41	413	0.0	518
Msa0282490	MtrunA17_Chr2g0329931	63.588	379	132	1	34	412	41	413	0.0	511
Msa0282490	MtrunA17_Chr8g0342861	63.061	379	134	1	34	412	41	413	1.55e-180	509
Msa0282490	MtrunA17_Chr4g0070981	63.852	379	131	1	34	412	41	413	1.12e-179	506
Msa0282490	MtrunA17_Chr1g0169331	42.569	397	185	6	52	412	9	398	3.25e-103	311
Msa0282490	MtrunA17_Chr8g0342851	43.466	352	163	5	63	412	2	319	7.02e-92	280
Msa0282490	MtrunA17_Chr7g0247191	38.743	382	224	4	26	405	140	513	3.38e-91	285
Msa0282490	MtrunA17_Chr7g0247181	38.220	382	226	4	26	405	130	503	8.66e-91	283
Msa0282490	MtrunA17_Chr7g0247221	38.220	382	226	4	26	405	133	506	1.22e-90	283

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282490	AT3G19760.1	85.024	414	54	2	1	412	1	408	0.0	724
Msa0282490	AT1G51380.1	72.589	394	99	2	19	412	8	392	0.0	602
Msa0282490	AT3G13920.3	64.644	379	128	1	34	412	30	402	0.0	516
Msa0282490	AT3G13920.1	64.644	379	128	1	34	412	40	412	0.0	516
Msa0282490	AT1G54270.1	63.852	379	131	1	34	412	40	412	0.0	510
Msa0282490	AT1G72730.1	63.325	379	133	1	34	412	42	414	5.27e-179	505
Msa0282490	AT3G13920.4	63.588	379	127	2	34	412	40	407	2.27e-177	501
Msa0282490	AT1G54270.2	62.797	379	130	2	34	412	40	407	5.79e-175	494
Msa0282490	AT3G13920.2	65.826	357	116	1	34	390	40	390	5.62e-173	490
Msa0282490	AT3G13920.5	65.826	357	116	1	34	390	44	394	8.68e-173	489

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Find 122 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCGTGATGAATTGACAT+TGG	0.623480	2_3:-18492250	Msa0282490:CDS
AACAACCTGACAAACCGTCA+AGG	0.644356	2_3:+18494446	None:intergenic
AACTAGCAACTTGATGGCCC+TGG	0.522591	2_3:+18493189	None:intergenic
AACTTGCCTGTTGTACATCA+AGG	0.552831	2_3:+18491728	None:intergenic
AAGAGAACCTGAAGATCCGG+TGG	0.623806	2_3:+18492995	None:intergenic
AAGCCCATGCTTGCATTGGA+GGG	0.587223	2_3:-18493315	Msa0282490:CDS
AAGGCCACGAGCCCAAACAT+CGG	0.689261	2_3:+18491747	None:intergenic
AAGGGAAAGAGATGCCATTA+TGG	0.307907	2_3:-18491809	Msa0282490:CDS
AAGGTTTCTCAAATCCGTAT+TGG	0.482981	2_3:+18494562	None:intergenic
AATAAGCTACGCCTTACGTT+TGG	0.311845	2_3:+18494799	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAATAAGTCAATCCAAAC+AGG	-	chr2_3:18491355-18491374	Msa0282490:intron	30.0%
CAGATGATTAAAAACATCTC+CGG	+	chr2_3:18491735-18491754	None:intergenic	30.0%
ACAGACTGAGAAAGTTATAT+TGG	-	chr2_3:18491887-18491906	Msa0282490:intron	30.0%
GAGAAAGTTATATTGGCAAT+TGG	-	chr2_3:18491894-18491913	Msa0282490:intron	30.0%
GAAAGTTATATTGGCAATTG+GGG	-	chr2_3:18491896-18491915	Msa0282490:intron	30.0%
AAACACAATGACAAAGATGA+GGG	+	chr2_3:18492290-18492309	None:intergenic	30.0%
AAAACACAATGACAAAGATG+AGG	+	chr2_3:18492291-18492310	None:intergenic	30.0%
TTAAAACAAACTAAGGTGAC+AGG	-	chr2_3:18492348-18492367	Msa0282490:intron	30.0%
TAAAACAAACTAAGGTGACA+GGG	-	chr2_3:18492349-18492368	Msa0282490:intron	30.0%
GAAAAAATAAGCAGCTACAT+TGG	+	chr2_3:18492591-18492610	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	18490431	18494840	18490431	ID=Msa0282490;Name=Msa0282490
chr2_3	mRNA	18490431	18494840	18490431	ID=Msa0282490-mRNA-1;Parent=Msa0282490;Name=Msa0282490-mRNA-1;_AED=0.11;_eAED=0.11;_QI=110\|0.85\|0.87\|1\|0.85\|0.87\|8\|368\|412
chr2_3	exon	18490431	18490912	18490431	ID=Msa0282490-mRNA-1:exon:7620;Parent=Msa0282490-mRNA-1
chr2_3	exon	18491100	18491186	18491100	ID=Msa0282490-mRNA-1:exon:7619;Parent=Msa0282490-mRNA-1
chr2_3	exon	18491717	18491902	18491717	ID=Msa0282490-mRNA-1:exon:7618;Parent=Msa0282490-mRNA-1
chr2_3	exon	18492047	18492166	18492047	ID=Msa0282490-mRNA-1:exon:7617;Parent=Msa0282490-mRNA-1
chr2_3	exon	18492248	18492355	18492248	ID=Msa0282490-mRNA-1:exon:7616;Parent=Msa0282490-mRNA-1
chr2_3	exon	18493003	18493083	18493003	ID=Msa0282490-mRNA-1:exon:7615;Parent=Msa0282490-mRNA-1
chr2_3	exon	18493186	18493426	18493186	ID=Msa0282490-mRNA-1:exon:7614;Parent=Msa0282490-mRNA-1
chr2_3	exon	18494429	18494840	18494429	ID=Msa0282490-mRNA-1:exon:7613;Parent=Msa0282490-mRNA-1
chr2_3	five_prime_UTR	18494731	18494840	18494731	ID=Msa0282490-mRNA-1:five_prime_utr;Parent=Msa0282490-mRNA-1
chr2_3	CDS	18494429	18494730	18494429	ID=Msa0282490-mRNA-1:cds;Parent=Msa0282490-mRNA-1
chr2_3	CDS	18493186	18493426	18493186	ID=Msa0282490-mRNA-1:cds;Parent=Msa0282490-mRNA-1
chr2_3	CDS	18493003	18493083	18493003	ID=Msa0282490-mRNA-1:cds;Parent=Msa0282490-mRNA-1
chr2_3	CDS	18492248	18492355	18492248	ID=Msa0282490-mRNA-1:cds;Parent=Msa0282490-mRNA-1
chr2_3	CDS	18492047	18492166	18492047	ID=Msa0282490-mRNA-1:cds;Parent=Msa0282490-mRNA-1
chr2_3	CDS	18491717	18491902	18491717	ID=Msa0282490-mRNA-1:cds;Parent=Msa0282490-mRNA-1
chr2_3	CDS	18491100	18491186	18491100	ID=Msa0282490-mRNA-1:cds;Parent=Msa0282490-mRNA-1
chr2_3	CDS	18490799	18490912	18490799	ID=Msa0282490-mRNA-1:cds;Parent=Msa0282490-mRNA-1
chr2_3	three_prime_UTR	18490431	18490798	18490431	ID=Msa0282490-mRNA-1:three_prime_utr;Parent=Msa0282490-mRNA-1

Gene Sequence

>Msa0282490

ATGGCGACAGCTTCTGTGGTTCCGGCGAATCGCCGACGAACGGTTACGGCGAACGAGGACATGGATTTCGAAACTACCGAAGGCGTGAAAGCAATCGGAAGCTTCGAAGAGATGGGGATAAAAGACGATTTGCTTCGTGGAATCTACCAATACGGATTTGAGAAACCTTCTGCGATTCAACAGCGTGCTGTGGCGCCGATTATTCAAGGAAGGGATGTTATTGCTCAGGCGCAATCTGGTACTGGAAAGACTTCGATGATTGCCTTGACGGTTTGTCAGGTTGTTGATACCTCCGTCAGAGAGGTGCAAGCTTTAATTGTGTCTCCGACAAGAGAACTGGCCTCACAGACTGAGAAAGTTATATTGGCAATTGGGGATTTTATCAATATACAAGCCCATGCTTGCATTGGAGGGAAAAGTGTGGGAGAAGACATTAGAAAACTTGAGCACGGAGTTCATGTCGTGTCTGGAACTCCTGGCCGAGTCTGTGACATGATAAAGAGGAGAACATTGCGCACCAGGGCCATCAAGTTGCTAGTTCTGGATGAATCTGATGAAATGTTGAGCAGAGGGTTTAAAGATCAGATTTATGATGTGTACAGATATCTCCCACCGGATCTTCAGGTTTGCCTGATTTCTGCTACGCTACCTCATGAAATTCTGGAGATGACAAACAAGTTTATGACAGATCCAGTGAGGATCCTTGTAAAGCGTGATGAATTGACATTGGAGGGCATTAAGCAATTTTTTGTTGCGGTTGAAAGGGAGGAATGGAAGTTTGATACTCTGTGTGATCTATATGATACTCTCACCATTACTCAAGCTGTTATATTCTGCAACACCAAGCGGAAGGTGGACTGGTTAACTGAAAAAATGCGTAACAATAATTTCACTGTTTCATCCATGCACGGTGACATGCCTCAAAGGGAAAGAGATGCCATTATGGGTGAATTCCGTGCTGGAACAACCCGAGTATTGATAACAACCGATGTTTGGGCTCGTGGCCTTGATGTACAACAGGCAAGTTATCTGTCCAGTGTTTCTCTAGTTATCAATTATGACCTTCCAAATAATCGAGAGCTCTACATTCATCGGATTGGTCGTTCTGGACGTTTTGGACGAAAGGGTGTTGCAATAAATTTTGTTAAAAGCGATGACATCAAGATTTTGAGAGATATTGAGCAATATTACAGTACCCAGATTGATGAGATGCCAATGAATGTTGCTGATCTTATATAA

Protein sequence

>Msa0282490

MATASVVPANRRRTVTANEDMDFETTEGVKAIGSFEEMGIKDDLLRGIYQYGFEKPSAIQQRAVAPIIQGRDVIAQAQSGTGKTSMIALTVCQVVDTSVREVQALIVSPTRELASQTEKVILAIGDFINIQAHACIGGKSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVCLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRNNNFTVSSMHGDMPQRERDAIMGEFRAGTTRVLITTDVWARGLDVQQASYLSSVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI*