Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0286860 | Msa0286890 | 0.847906 | 8.594817e-60 | -8.615850e-47 |
| Msa0286880 | Msa0286890 | 0.846382 | 2.249000e-59 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAACCAACGAGGTGATTC+TGG | 0.232258 | 2_3:+28699908 | None:intergenic |
| AGCTTTACTTAACTTCGATT+CGG | 0.432129 | 2_3:-28699803 | Msa0286890:CDS |
| GGAACATGGAGGTGGATGTT+AGG | 0.455461 | 2_3:-28699980 | None:intergenic |
| ACCTCGTTGGTTGTTCAGAA+AGG | 0.461288 | 2_3:-28699900 | Msa0286890:intron |
| ATTTCATCTTCAACATCTTG+TGG | 0.465872 | 2_3:+28699713 | None:intergenic |
| GTTAGGCGTTGCAGCAGTAC+CGG | 0.494152 | 2_3:-28699963 | Msa0286890:CDS |
| ACAACCAACGAGGTGATTCT+GGG | 0.512931 | 2_3:+28699909 | None:intergenic |
| CATCTTCAACATCTTGTGGA+GGG | 0.542544 | 2_3:+28699717 | None:intergenic |
| AATACGATTTGTGTTAAAGC+CGG | 0.560109 | 2_3:+28699944 | None:intergenic |
| TGCTCCCAGAATCACCTCGT+TGG | 0.561859 | 2_3:-28699913 | Msa0286890:CDS |
| AGGCACCAGGAACATGGAGG+TGG | 0.563216 | 2_3:-28699988 | None:intergenic |
| TCATCTTCAACATCTTGTGG+AGG | 0.583395 | 2_3:+28699716 | None:intergenic |
| ACCTTTCTGAACAACCAACG+AGG | 0.733259 | 2_3:+28699899 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATATGTTAAACGAAAAAC+AGG | - | chr2_3:28699898-28699917 | Msa0286890:intron | 20.0% |
| !! | TATATGTTAAACGAAAAACA+GGG | - | chr2_3:28699899-28699918 | Msa0286890:intron | 20.0% |
| !!! | AAGAAGCTAAAGAAATTTTA+AGG | - | chr2_3:28699930-28699949 | Msa0286890:CDS | 20.0% |
| AATACGATTTGTGTTAAAGC+CGG | + | chr2_3:28699736-28699755 | None:intergenic | 30.0% | |
| AATTAGATTACATGCTCTCA+TGG | + | chr2_3:28699834-28699853 | None:intergenic | 30.0% | |
| AGCTTTACTTAACTTCGATT+CGG | - | chr2_3:28699874-28699893 | Msa0286890:intron | 30.0% | |
| ATTTCATCTTCAACATCTTG+TGG | + | chr2_3:28699967-28699986 | None:intergenic | 30.0% | |
| CATCTTCAACATCTTGTGGA+GGG | + | chr2_3:28699963-28699982 | None:intergenic | 40.0% | |
| TCATCTTCAACATCTTGTGG+AGG | + | chr2_3:28699964-28699983 | None:intergenic | 40.0% | |
| ACAACCAACGAGGTGATTCT+GGG | + | chr2_3:28699771-28699790 | None:intergenic | 45.0% | |
| AACAACCAACGAGGTGATTC+TGG | + | chr2_3:28699772-28699791 | None:intergenic | 45.0% | |
| ! | ACCTCGTTGGTTGTTCAGAA+AGG | - | chr2_3:28699777-28699796 | Msa0286890:CDS | 45.0% |
| ACCTTTCTGAACAACCAACG+AGG | + | chr2_3:28699781-28699800 | None:intergenic | 45.0% | |
| ! | GTTAGGCGTTGCAGCAGTAC+CGG | - | chr2_3:28699714-28699733 | Msa0286890:CDS | 55.0% |
| TGCTCCCAGAATCACCTCGT+TGG | - | chr2_3:28699764-28699783 | Msa0286890:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 28699702 | 28699997 | 28699702 | ID=Msa0286890;Name=Msa0286890 |
| chr2_3 | mRNA | 28699702 | 28699997 | 28699702 | ID=Msa0286890-mRNA-1;Parent=Msa0286890;Name=Msa0286890-mRNA-1;_AED=0.42;_eAED=0.90;_QI=0|0|0|1|0|0|2|0|72 |
| chr2_3 | exon | 28699901 | 28699997 | 28699901 | ID=Msa0286890-mRNA-1:exon:10148;Parent=Msa0286890-mRNA-1 |
| chr2_3 | exon | 28699702 | 28699823 | 28699702 | ID=Msa0286890-mRNA-1:exon:10147;Parent=Msa0286890-mRNA-1 |
| chr2_3 | CDS | 28699901 | 28699997 | 28699901 | ID=Msa0286890-mRNA-1:cds;Parent=Msa0286890-mRNA-1 |
| chr2_3 | CDS | 28699702 | 28699823 | 28699702 | ID=Msa0286890-mRNA-1:cds;Parent=Msa0286890-mRNA-1 |
| Gene Sequence |
| Protein sequence |
