AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0286860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0286860 MtrunA17_Chr2g0303731 82.883 333 7 2 1 283 248 580 0.0 535
Msa0286860 MtrunA17_Chr8g0376631 72.340 329 40 3 1 279 248 575 1.38e-154 443
Msa0286860 MtrunA17_Chr8g0376611 65.528 322 62 3 1 274 181 501 7.52e-138 398
Msa0286860 MtrunA17_Chr2g0303701 57.669 326 82 5 1 274 248 569 2.78e-119 353
Msa0286860 MtrunA17_Chr8g0376601 56.740 319 88 3 2 271 249 566 8.93e-116 344
Msa0286860 MtrunA17_Chr2g0290181 48.464 293 100 3 23 265 274 565 1.04e-85 267
Msa0286860 MtrunA17_Chr1g0213611 48.944 284 108 4 4 283 300 550 5.31e-82 256
Msa0286860 MtrunA17_Chr5g0434251 73.077 156 37 2 123 274 3 157 6.01e-81 241
Msa0286860 MtrunA17_Chr7g0214451 47.751 289 107 5 1 282 249 500 1.02e-78 247
Msa0286860 MtrunA17_Chr5g0434261 44.340 318 82 4 2 270 56 327 1.73e-77 238
Msa0286860 MtrunA17_Chr2g0290161 47.099 293 104 4 23 265 181 472 2.03e-77 243
Msa0286860 MtrunA17_Chr1g0213631 47.535 284 113 4 2 280 204 456 6.79e-76 238
Msa0286860 MtrunA17_Chr3g0121674 43.509 285 118 6 4 283 61 307 1.68e-73 227
Msa0286860 MtrunA17_Chr6g0450291 75.610 123 30 0 1 123 1 123 3.04e-55 176
Msa0286860 MtrunA17_Chr6g0450281 75.610 123 30 0 1 123 1 123 9.36e-55 176
Msa0286860 MtrunA17_Chr6g0450271 75.610 123 30 0 1 123 57 179 1.94e-54 177
Msa0286860 MtrunA17_Chr6g0450241 74.797 123 31 0 1 123 1 123 2.99e-54 174
Msa0286860 MtrunA17_Chr5g0434241 79.208 101 21 0 1 101 1 101 1.01e-51 165
Msa0286860 MtrunA17_Chr4g0021771 34.979 243 118 5 22 259 298 505 4.63e-30 118
Msa0286860 MtrunA17_Chr2g0282031 31.301 246 131 3 17 258 291 502 4.88e-28 112
Msa0286860 MtrunA17_Chr5g0394341 29.588 267 142 7 16 274 274 502 4.76e-27 109
Msa0286860 MtrunA17_Chr1g0205671 29.600 250 131 6 27 268 284 496 1.11e-25 105
Msa0286860 MtrunA17_Chr7g0250881 31.169 231 118 4 30 256 309 502 2.05e-25 105
Msa0286860 MtrunA17_Chr7g0273261 30.502 259 137 6 3 257 19 238 3.00e-25 101
Msa0286860 MtrunA17_Chr7g0259221 32.143 252 128 8 11 257 263 476 3.77e-25 104
Msa0286860 MtrunA17_Chr4g0039651 30.924 249 130 4 10 254 288 498 5.55e-25 103
Msa0286860 MtrunA17_Chr7g0259471 33.058 242 118 7 21 256 270 473 9.24e-25 103
Msa0286860 MtrunA17_Chr1g0205691 29.104 268 152 6 16 279 274 507 1.04e-24 103
Msa0286860 MtrunA17_Chr1g0155221 29.297 256 141 7 7 254 263 486 1.34e-24 102
Msa0286860 MtrunA17_Chr6g0457781 31.034 232 119 4 30 257 349 543 2.20e-24 102
Msa0286860 MtrunA17_Chr3g0144361 30.252 238 132 4 20 253 281 488 2.40e-24 102
Msa0286860 MtrunA17_Chr8g0373181 30.769 260 135 9 4 253 258 482 6.82e-24 100
Msa0286860 MtrunA17_Chr4g0036021 28.077 260 147 5 17 272 274 497 8.78e-24 100
Msa0286860 MtrunA17_Chr1g0205681 27.668 253 138 6 24 268 282 497 1.69e-23 99.8
Msa0286860 MtrunA17_Chr3g0144261 28.195 266 124 6 8 250 490 711 7.66e-23 98.2
Msa0286860 MtrunA17_Chr4g0034681 27.306 271 139 6 17 274 278 503 8.77e-23 97.8
Msa0286860 MtrunA17_Chr5g0432581 30.364 247 138 4 17 259 269 485 1.17e-22 97.1
Msa0286860 MtrunA17_Chr3g0146061 30.290 241 121 5 23 250 515 721 2.26e-22 96.7
Msa0286860 MtrunA17_Chr8g0373531 29.839 248 138 5 17 259 269 485 4.10e-22 95.5
Msa0286860 MtrunA17_Chr7g0273281 28.346 254 143 6 3 253 275 492 1.09e-21 94.4
Msa0286860 MtrunA17_Chr3g0128081 25.954 262 160 5 17 273 274 506 1.33e-21 94.4
Msa0286860 MtrunA17_Chr8g0370151 26.038 265 138 5 23 272 136 357 1.89e-21 92.4
Msa0286860 MtrunA17_Chr4g0065311 28.000 275 152 9 1 268 255 490 2.11e-21 93.6
Msa0286860 MtrunA17_Chr4g0032021 26.459 257 147 4 1 253 333 551 1.03e-20 91.7
Msa0286860 MtrunA17_Chr5g0420841 25.279 269 157 7 16 274 272 506 1.67e-20 90.9
Msa0286860 MtrunA17_Chr8g0390141 28.244 262 153 6 17 274 277 507 2.27e-20 90.5
Msa0286860 MtrunA17_Chr3g0128171 26.515 264 163 6 5 265 216 451 3.14e-20 90.1
Msa0286860 MtrunA17_Chr8g0373191 28.736 261 143 8 7 258 233 459 3.99e-20 89.7
Msa0286860 MtrunA17_Chr4g0048051 28.571 259 151 7 18 273 275 502 4.42e-20 89.7
Msa0286860 MtrunA17_Chr3g0085971 24.060 266 166 6 16 275 272 507 6.54e-20 89.4
Msa0286860 MtrunA17_Chr8g0390521 28.395 243 139 2 17 255 259 470 1.45e-19 88.2
Msa0286860 MtrunA17_Chr4g0066581 27.953 254 143 6 11 260 273 490 1.94e-19 87.8
Msa0286860 MtrunA17_Chr2g0286721 29.482 251 135 8 11 256 270 483 3.97e-19 87.0
Msa0286860 MtrunA17_Chr5g0419321 25.806 248 137 5 16 250 516 729 1.56e-18 85.5
Msa0286860 MtrunA17_Chr3g0115721 28.897 263 146 8 21 275 277 506 3.50e-18 84.3
Msa0286860 MtrunA17_Chr6g0485661 28.139 231 132 4 20 246 300 500 3.82e-18 84.0
Msa0286860 MtrunA17_Chr4g0065324 28.369 282 156 10 1 275 263 505 3.94e-18 84.0
Msa0286860 MtrunA17_Chr3g0115751 25.379 264 162 6 16 276 277 508 1.10e-17 82.8
Msa0286860 MtrunA17_Chr3g0078991 26.820 261 156 6 16 272 275 504 1.28e-17 82.4
Msa0286860 MtrunA17_Chr3g0078971 28.309 272 155 10 1 268 260 495 1.31e-17 82.4
Msa0286860 MtrunA17_Chr5g0407441 25.000 248 139 5 16 250 490 703 1.64e-17 82.4
Msa0286860 MtrunA17_Chr4g0065351 26.515 264 147 8 21 275 117 342 1.85e-17 81.3
Msa0286860 MtrunA17_Chr3g0115731 28.125 256 139 8 21 268 256 474 1.33e-16 79.3
Msa0286860 MtrunA17_Chr7g0257631 28.151 238 126 8 37 267 330 529 1.39e-15 76.6
Msa0286860 MtrunA17_Chr4g0004821 28.016 257 138 7 2 253 255 469 1.70e-15 76.3
Msa0286860 MtrunA17_Chr5g0445601 27.737 274 152 9 1 267 261 495 2.53e-15 75.9
Msa0286860 MtrunA17_Chr3g0115741 24.514 257 155 5 16 265 275 499 3.13e-15 75.5
Msa0286860 MtrunA17_Chr5g0404181 34.426 122 66 4 144 253 9 128 2.50e-13 65.9
Msa0286860 MtrunA17_Chr3g0115781 25.969 258 150 8 16 265 274 498 4.87e-13 68.9
Msa0286860 MtrunA17_Chr3g0115711 23.047 256 159 6 21 270 276 499 5.98e-13 68.6
Msa0286860 MtrunA17_Chr3g0078791 23.361 244 125 6 18 252 220 410 1.61e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0286860 AT1G30220.1 61.371 321 71 3 2 270 251 570 1.09e-133 390
Msa0286860 AT4G16480.2 47.751 289 96 3 23 257 274 561 1.15e-86 270
Msa0286860 AT4G16480.3 47.751 289 96 3 23 257 274 561 1.15e-86 270
Msa0286860 AT4G16480.1 47.751 289 96 3 23 257 274 561 1.15e-86 270
Msa0286860 AT2G35740.1 43.770 313 118 4 2 257 249 560 5.84e-85 265
Msa0286860 AT2G43330.1 48.746 279 108 4 2 275 251 499 2.88e-80 251
Msa0286860 AT2G18480.1 36.402 239 115 5 22 255 276 482 5.12e-31 121
Msa0286860 AT2G48020.2 31.349 252 130 7 11 257 249 462 3.10e-28 113
Msa0286860 AT2G48020.1 31.349 252 130 7 11 257 249 462 3.10e-28 113
Msa0286860 AT2G20780.1 30.742 283 134 7 4 259 260 507 7.51e-27 109
Msa0286860 AT2G20780.2 30.742 283 134 7 4 259 202 449 1.38e-26 108
Msa0286860 AT3G51490.2 32.143 252 124 5 16 254 501 718 6.84e-26 107
Msa0286860 AT3G51490.3 32.143 252 124 5 16 254 451 668 7.89e-26 107
Msa0286860 AT5G18840.1 32.937 252 124 9 15 259 268 481 1.13e-25 105
Msa0286860 AT3G05150.2 31.698 265 136 9 11 269 256 481 1.24e-25 105
Msa0286860 AT3G05150.1 32.143 252 127 8 11 256 256 469 1.88e-25 105
Msa0286860 AT3G05150.3 32.143 252 127 8 11 256 256 469 1.88e-25 105
Msa0286860 AT4G36670.1 30.000 270 139 7 1 258 250 481 4.34e-25 104
Msa0286860 AT4G35300.5 31.923 260 124 5 7 250 301 523 1.94e-24 102
Msa0286860 AT1G75220.1 29.730 259 147 6 4 257 259 487 2.62e-24 102
Msa0286860 AT4G35300.4 31.923 260 124 5 7 250 498 720 3.43e-24 102
Msa0286860 AT4G35300.8 31.923 260 124 5 7 250 498 720 3.43e-24 102
Msa0286860 AT4G35300.6 31.923 260 124 5 7 250 498 720 3.43e-24 102
Msa0286860 AT4G35300.11 31.923 260 124 5 7 250 498 720 3.43e-24 102
Msa0286860 AT4G35300.1 31.923 260 124 5 7 250 498 720 3.43e-24 102
Msa0286860 AT4G35300.10 31.923 260 124 5 7 250 498 720 3.43e-24 102
Msa0286860 AT4G35300.7 31.923 260 124 5 7 250 498 720 3.43e-24 102
Msa0286860 AT4G35300.9 31.923 260 124 5 7 250 488 710 3.79e-24 102
Msa0286860 AT4G35300.2 31.923 260 124 5 7 250 488 710 3.79e-24 102
Msa0286860 AT4G35300.3 31.923 260 124 5 7 250 488 710 3.79e-24 102
Msa0286860 AT5G26340.1 27.925 265 150 5 24 280 282 513 1.18e-23 100
Msa0286860 AT2G16130.1 30.705 241 134 4 20 256 278 489 1.93e-23 99.8
Msa0286860 AT3G05960.1 27.046 281 167 6 5 280 259 506 2.52e-23 99.8
Msa0286860 AT3G18830.1 28.077 260 141 5 20 272 288 508 3.27e-23 99.4
Msa0286860 AT3G05155.1 28.740 254 137 7 7 254 241 456 5.36e-23 98.2
Msa0286860 AT2G16120.1 29.461 241 137 4 20 256 278 489 2.44e-22 96.7
Msa0286860 AT3G51490.1 31.837 245 126 5 16 254 501 710 2.54e-22 97.1
Msa0286860 AT1G54730.2 27.843 255 143 6 3 253 245 462 3.22e-22 96.3
Msa0286860 AT5G26250.1 28.623 276 159 6 5 275 260 502 3.97e-22 96.3
Msa0286860 AT5G27360.2 31.418 261 138 6 3 259 144 367 4.02e-22 95.1
Msa0286860 AT5G27360.5 31.418 261 138 6 3 259 144 367 4.02e-22 95.1
Msa0286860 AT5G27360.3 31.418 261 138 6 3 259 296 519 4.33e-22 95.9
Msa0286860 AT5G27360.1 31.418 261 138 6 3 259 248 471 5.97e-22 95.5
Msa0286860 AT3G03090.1 29.218 243 130 4 16 254 296 500 2.15e-21 94.0
Msa0286860 AT5G16150.2 30.736 231 119 4 30 256 349 542 2.29e-21 94.0
Msa0286860 AT5G16150.1 30.736 231 119 4 30 256 349 542 2.29e-21 94.0
Msa0286860 AT5G16150.3 30.736 231 119 4 30 256 349 542 2.29e-21 94.0
Msa0286860 AT5G17010.1 28.916 249 135 4 10 254 290 500 3.22e-21 93.6
Msa0286860 AT5G17010.3 28.916 249 135 4 10 254 290 500 3.22e-21 93.6
Msa0286860 AT5G17010.4 28.916 249 135 4 10 254 290 500 3.22e-21 93.6
Msa0286860 AT5G59250.1 28.755 233 125 3 25 253 358 553 1.46e-20 91.7
Msa0286860 AT1G20840.2 31.395 258 119 6 23 265 507 721 1.97e-20 91.7
Msa0286860 AT1G20840.1 31.395 258 119 6 23 265 507 721 1.97e-20 91.7
Msa0286860 AT1G19450.1 28.897 263 144 7 4 257 260 488 3.13e-20 90.5
Msa0286860 AT1G79820.2 28.906 256 137 6 2 253 273 487 6.64e-20 89.7
Msa0286860 AT1G79820.1 28.906 256 137 6 2 253 273 487 6.64e-20 89.7
Msa0286860 AT3G05400.1 28.627 255 138 7 3 253 242 456 1.30e-19 88.6
Msa0286860 AT3G05400.2 28.627 255 138 7 3 253 222 436 1.87e-19 88.2
Msa0286860 AT4G21480.1 27.273 253 150 5 18 265 273 496 3.03e-19 87.8
Msa0286860 AT1G07340.1 29.119 261 147 6 13 268 267 494 5.45e-19 87.0
Msa0286860 AT1G11260.1 25.461 271 165 7 16 280 274 513 6.35e-19 86.7
Msa0286860 AT1G08930.1 27.615 239 131 5 27 260 294 495 2.76e-18 84.7
Msa0286860 AT1G08930.2 27.615 239 131 5 27 260 294 495 2.76e-18 84.7
Msa0286860 AT5G27350.1 30.588 255 136 6 3 253 244 461 9.20e-18 83.2
Msa0286860 AT5G61520.2 25.899 278 168 6 1 275 216 458 8.68e-17 80.5
Msa0286860 AT5G61520.1 25.899 278 168 6 1 275 264 506 1.64e-16 79.7
Msa0286860 AT4G04750.3 29.825 228 120 7 30 253 321 512 1.99e-16 79.3
Msa0286860 AT4G04750.1 29.310 232 124 6 27 254 274 469 3.05e-16 79.0
Msa0286860 AT4G04750.2 29.310 232 124 6 27 254 274 469 3.05e-16 79.0
Msa0286860 AT4G04760.3 29.565 230 122 7 30 255 214 407 7.21e-16 77.4
Msa0286860 AT4G04760.1 29.437 231 123 7 30 256 273 467 1.07e-15 77.0
Msa0286860 AT1G54730.5 24.706 255 130 6 3 253 245 441 2.26e-15 76.3
Msa0286860 AT1G08920.1 28.571 231 125 5 30 256 271 465 5.17e-15 75.1
Msa0286860 AT1G08920.2 28.139 231 133 6 30 256 271 472 9.34e-15 74.3
Msa0286860 AT1G08900.5 26.991 226 125 5 37 258 229 418 1.10e-14 73.9
Msa0286860 AT1G08900.3 26.991 226 125 5 37 258 263 452 1.21e-14 73.9
Msa0286860 AT1G08900.1 26.991 226 125 5 37 258 271 460 1.21e-14 73.9
Msa0286860 AT1G08900.2 26.991 226 125 5 37 258 271 460 1.21e-14 73.9
Msa0286860 AT1G08890.1 25.221 226 125 6 37 256 273 460 3.01e-14 72.8
Msa0286860 AT4G04760.2 28.958 259 140 11 2 256 246 464 1.66e-13 70.9
Msa0286860 AT1G79820.3 28.333 180 91 3 78 253 294 439 8.84e-12 65.5
Msa0286860 AT1G79820.5 28.333 180 91 3 78 253 232 377 1.83e-11 64.3

Find 40 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGGAAGTCCCTTTGTTTC+TGG 0.144531 2_3:+28682869 None:intergenic
CATATTCCTCTGTATCTTAA+TGG 0.207748 2_3:+28683031 None:intergenic
TAAACCTCTTCTTACTGTTT+TGG 0.262901 2_3:+28684125 None:intergenic
CTATCATTGACTGATGCTAT+TGG 0.289689 2_3:-28682950 Msa0286860:CDS
CATGGAACTGTTCCCATTCC+TGG 0.335209 2_3:+28683076 None:intergenic
CAGCAAAAGTTTCTTCCTTC+CGG 0.336912 2_3:+28683918 None:intergenic
GAGAATGAGTTGTAGTTTGA+TGG 0.346522 2_3:+28683845 None:intergenic
GTTGGAAGACGAATCGTCTT+TGG 0.354197 2_3:+28684176 None:intergenic
GAATAGCATCAACATCAAAT+TGG 0.360652 2_3:-28683003 Msa0286860:CDS
TTTGATTATGCTGACTTTGT+TGG 0.388815 2_3:+28684158 None:intergenic
CCATTCCTGGTGAGAAGAAT+AGG 0.401944 2_3:+28683089 None:intergenic
ACAGCTGGAATCAATGCATT+TGG 0.414045 2_3:-28683973 Msa0286860:CDS
AACGGTTGGCTAGCACTAAT+CGG 0.428488 2_3:-28683127 Msa0286860:CDS
ATTATTGTGCAGCTATTACC+TGG 0.428833 2_3:-28683238 Msa0286860:intron
CCTATTCTTCTCACCAGGAA+TGG 0.445918 2_3:-28683089 Msa0286860:CDS
TGAAGAGGTTGAGAAAATGT+TGG 0.471924 2_3:-28682846 Msa0286860:CDS
CTATTCTTCTCACCAGGAAT+GGG 0.473967 2_3:-28683088 Msa0286860:CDS
ATTTCAGAGTTTACAACCCA+TGG 0.480742 2_3:+28683058 None:intergenic
CCAACCATCGTTCAACTAGC+TGG 0.480899 2_3:-28684036 Msa0286860:CDS
TGTTGATTCCGACGAATTGT+TGG 0.492825 2_3:+28684076 None:intergenic
GTAAGAAGAGGTTTATATGC+AGG 0.494340 2_3:-28684117 Msa0286860:CDS
CTTCTGTCACTCGTCACAGC+TGG 0.501982 2_3:-28683988 Msa0286860:CDS
AACTACAACTCATTCTCCTA+TGG 0.511200 2_3:-28683839 Msa0286860:intron
TTATTGTGCAGCTATTACCT+GGG 0.530370 2_3:-28683237 Msa0286860:intron
CAGGAATGGGAACAGTTCCA+TGG 0.531089 2_3:-28683075 Msa0286860:CDS
AAAACCAAAACAGTAAGAAG+AGG 0.540201 2_3:-28684129 Msa0286860:CDS
TTAAGATACAGAGGAATATG+TGG 0.557495 2_3:-28683028 Msa0286860:CDS
AGAGGTTTATATGCAGGAGT+AGG 0.565880 2_3:-28684111 Msa0286860:CDS
CCAGCTAGTTGAACGATGGT+TGG 0.571403 2_3:+28684036 None:intergenic
AAAGGGACTTCCTATTGAAG+AGG 0.575338 2_3:-28682861 Msa0286860:CDS
AGGAATGGGAACAGTTCCAT+GGG 0.596384 2_3:-28683074 Msa0286860:CDS
TACATCCTATTCTTCTCACC+AGG 0.604815 2_3:-28683094 Msa0286860:CDS
CAAATCTTCCAACAATTCGT+CGG 0.617999 2_3:-28684084 Msa0286860:CDS
TTTGATATTAGACATGCCCC+AGG 0.618868 2_3:+28683220 None:intergenic
TATTGTGCAGCTATTACCTG+GGG 0.645299 2_3:-28683236 Msa0286860:intron
AGTCGACAATGGTACACAAG+AGG 0.654966 2_3:-28683163 Msa0286860:CDS
AGATACAGAGGAATATGTGG+AGG 0.659965 2_3:-28683025 Msa0286860:CDS
TCTAATATCAAATGACACAA+CGG 0.660534 2_3:-28683209 Msa0286860:CDS
ATATATCCATTAAGATACAG+AGG 0.661567 2_3:-28683037 Msa0286860:CDS
AAAACCAGCTAGTTGAACGA+TGG 0.712111 2_3:+28684032 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATATTGTAATTCAATAT+TGG + chr2_3:28683543-28683562 None:intergenic 15.0%
!! TCAATATTGTAATTCAATAT+TGG + chr2_3:28683543-28683562 None:intergenic 15.0%
!!! GAATTTTGTATTTTAAGTTT+TGG - chr2_3:28684129-28684148 Msa0286860:CDS 15.0%
!! TCAATATTGTAATTCAATAT+TGG + chr2_3:28683543-28683562 None:intergenic 15.0%
!! TCAATATTGTAATTCAATAT+TGG + chr2_3:28683543-28683562 None:intergenic 15.0%
!!! GAATTTTGTATTTTAAGTTT+TGG - chr2_3:28684129-28684148 Msa0286860:CDS 15.0%
!!! ATATATTTTATTGACAAGAC+CGG - chr2_3:28683009-28683028 Msa0286860:CDS 20.0%
!!! CACTTAGTATTTTATTTGAA+AGG + chr2_3:28683292-28683311 None:intergenic 20.0%
!! TAATTCTGTTAAAAAACACT+TGG + chr2_3:28683417-28683436 None:intergenic 20.0%
!!! GTAATTCAATATTGGTTATT+AGG + chr2_3:28683535-28683554 None:intergenic 20.0%
!!! GTAATTCAATATTGGTTATT+AGG + chr2_3:28683535-28683554 None:intergenic 20.0%
!!! TTAGTATTGTTATTAGACAT+TGG + chr2_3:28683689-28683708 None:intergenic 20.0%
!!! ATATATTTTATTGACAAGAC+CGG - chr2_3:28683009-28683028 Msa0286860:CDS 20.0%
!!! CACTTAGTATTTTATTTGAA+AGG + chr2_3:28683292-28683311 None:intergenic 20.0%
!! TAATTCTGTTAAAAAACACT+TGG + chr2_3:28683417-28683436 None:intergenic 20.0%
!!! GTAATTCAATATTGGTTATT+AGG + chr2_3:28683535-28683554 None:intergenic 20.0%
!!! GTAATTCAATATTGGTTATT+AGG + chr2_3:28683535-28683554 None:intergenic 20.0%
!!! TTAGTATTGTTATTAGACAT+TGG + chr2_3:28683689-28683708 None:intergenic 20.0%
!!! ACACTTGGTATTCTTTTTTT+TGG + chr2_3:28683402-28683421 None:intergenic 25.0%
!! ATAGTACGGTTAATTTTGTA+AGG + chr2_3:28683498-28683517 None:intergenic 25.0%
!! TGGTTAGTTAACTAATAGTA+CGG + chr2_3:28683512-28683531 None:intergenic 25.0%
!! ATTCAATATTGGTTATTAGG+TGG + chr2_3:28683532-28683551 None:intergenic 25.0%
!! ATAGTACGGTTAATTTTGTA+AGG + chr2_3:28683498-28683517 None:intergenic 25.0%
!! TGGTTAGTTAACTAATAGTA+CGG + chr2_3:28683512-28683531 None:intergenic 25.0%
!! ATTCAATATTGGTTATTAGG+TGG + chr2_3:28683532-28683551 None:intergenic 25.0%
! TCTAATATCAAATGACACAA+CGG - chr2_3:28683737-28683756 Msa0286860:intron 25.0%
! ATATATCCATTAAGATACAG+AGG - chr2_3:28683909-28683928 Msa0286860:CDS 25.0%
!!! ACACTTGGTATTCTTTTTTT+TGG + chr2_3:28683402-28683421 None:intergenic 25.0%
!! ATAGTACGGTTAATTTTGTA+AGG + chr2_3:28683498-28683517 None:intergenic 25.0%
!! TGGTTAGTTAACTAATAGTA+CGG + chr2_3:28683512-28683531 None:intergenic 25.0%
!! ATTCAATATTGGTTATTAGG+TGG + chr2_3:28683532-28683551 None:intergenic 25.0%
!! ATAGTACGGTTAATTTTGTA+AGG + chr2_3:28683498-28683517 None:intergenic 25.0%
!! TGGTTAGTTAACTAATAGTA+CGG + chr2_3:28683512-28683531 None:intergenic 25.0%
!! ATTCAATATTGGTTATTAGG+TGG + chr2_3:28683532-28683551 None:intergenic 25.0%
! TCTAATATCAAATGACACAA+CGG - chr2_3:28683737-28683756 Msa0286860:intron 25.0%
! ATATATCCATTAAGATACAG+AGG - chr2_3:28683909-28683928 Msa0286860:CDS 25.0%
!! TTTGATTATGCTGACTTTGT+TGG + chr2_3:28682791-28682810 None:intergenic 30.0%
AAAACCAAAACAGTAAGAAG+AGG - chr2_3:28682817-28682836 Msa0286860:CDS 30.0%
! TAAACCTCTTCTTACTGTTT+TGG + chr2_3:28682824-28682843 None:intergenic 30.0%
GTTTCAATTTCACTAACCAT+AGG + chr2_3:28683126-28683145 None:intergenic 30.0%
GACAATGCATAATATATGCA+AGG - chr2_3:28683351-28683370 Msa0286860:intron 30.0%
!!! CTTGGTATTCTTTTTTTTGG+TGG + chr2_3:28683399-28683418 None:intergenic 30.0%
CATATTCCTCTGTATCTTAA+TGG + chr2_3:28683918-28683937 None:intergenic 30.0%
TTAAGATACAGAGGAATATG+TGG - chr2_3:28683918-28683937 Msa0286860:CDS 30.0%
GAATAGCATCAACATCAAAT+TGG - chr2_3:28683943-28683962 Msa0286860:CDS 30.0%
! ATTTTCTCAACCTCTTCAAT+AGG + chr2_3:28684098-28684117 None:intergenic 30.0%
!!! TGTATTTTAAGTTTTGGCAG+AGG - chr2_3:28684135-28684154 Msa0286860:CDS 30.0%
!! TTTGATTATGCTGACTTTGT+TGG + chr2_3:28682791-28682810 None:intergenic 30.0%
AAAACCAAAACAGTAAGAAG+AGG - chr2_3:28682817-28682836 Msa0286860:CDS 30.0%
! TAAACCTCTTCTTACTGTTT+TGG + chr2_3:28682824-28682843 None:intergenic 30.0%
GTTTCAATTTCACTAACCAT+AGG + chr2_3:28683126-28683145 None:intergenic 30.0%
GACAATGCATAATATATGCA+AGG - chr2_3:28683351-28683370 Msa0286860:intron 30.0%
!!! CTTGGTATTCTTTTTTTTGG+TGG + chr2_3:28683399-28683418 None:intergenic 30.0%
CATATTCCTCTGTATCTTAA+TGG + chr2_3:28683918-28683937 None:intergenic 30.0%
TTAAGATACAGAGGAATATG+TGG - chr2_3:28683918-28683937 Msa0286860:CDS 30.0%
GAATAGCATCAACATCAAAT+TGG - chr2_3:28683943-28683962 Msa0286860:CDS 30.0%
! ATTTTCTCAACCTCTTCAAT+AGG + chr2_3:28684098-28684117 None:intergenic 30.0%
!!! TGTATTTTAAGTTTTGGCAG+AGG - chr2_3:28684135-28684154 Msa0286860:CDS 30.0%
GTAAGAAGAGGTTTATATGC+AGG - chr2_3:28682829-28682848 Msa0286860:CDS 35.0%
CAAATCTTCCAACAATTCGT+CGG - chr2_3:28682862-28682881 Msa0286860:CDS 35.0%
! ATTTTATTGACAAGACCGGA+AGG - chr2_3:28683013-28683032 Msa0286860:CDS 35.0%
!!! CTTTTGCTGTTTAGTTTGTC+TGG - chr2_3:28683042-28683061 Msa0286860:CDS 35.0%
GAGAATGAGTTGTAGTTTGA+TGG + chr2_3:28683104-28683123 None:intergenic 35.0%
AACTACAACTCATTCTCCTA+TGG - chr2_3:28683107-28683126 Msa0286860:CDS 35.0%
CCAAAGCTGTTCTATAATCA+GGG + chr2_3:28683170-28683189 None:intergenic 35.0%
TCCAAAGCTGTTCTATAATC+AGG + chr2_3:28683171-28683190 None:intergenic 35.0%
TGCATAATATATGCAAGGTC+CGG - chr2_3:28683356-28683375 Msa0286860:intron 35.0%
!!! GGTATTCTTTTTTTTGGTGG+TGG + chr2_3:28683396-28683415 None:intergenic 35.0%
ATTATTGTGCAGCTATTACC+TGG - chr2_3:28683708-28683727 Msa0286860:intron 35.0%
TTATTGTGCAGCTATTACCT+GGG - chr2_3:28683709-28683728 Msa0286860:intron 35.0%
AAAAAGAGCAAAGTCGACAA+TGG - chr2_3:28683772-28683791 Msa0286860:intron 35.0%
ATTTCAGAGTTTACAACCCA+TGG + chr2_3:28683891-28683910 None:intergenic 35.0%
! CTATCATTGACTGATGCTAT+TGG - chr2_3:28683996-28684015 Msa0286860:CDS 35.0%
!! TGTTTCTGGCACAAAAACAA+TGG + chr2_3:28684066-28684085 None:intergenic 35.0%
!! TGTTTTTGTGCCAGAAACAA+AGG - chr2_3:28684067-28684086 Msa0286860:CDS 35.0%
! GTTTTTGTGCCAGAAACAAA+GGG - chr2_3:28684068-28684087 Msa0286860:CDS 35.0%
TGAAGAGGTTGAGAAAATGT+TGG - chr2_3:28684100-28684119 Msa0286860:CDS 35.0%
GTAAGAAGAGGTTTATATGC+AGG - chr2_3:28682829-28682848 Msa0286860:CDS 35.0%
CAAATCTTCCAACAATTCGT+CGG - chr2_3:28682862-28682881 Msa0286860:CDS 35.0%
! ATTTTATTGACAAGACCGGA+AGG - chr2_3:28683013-28683032 Msa0286860:CDS 35.0%
!!! CTTTTGCTGTTTAGTTTGTC+TGG - chr2_3:28683042-28683061 Msa0286860:CDS 35.0%
GAGAATGAGTTGTAGTTTGA+TGG + chr2_3:28683104-28683123 None:intergenic 35.0%
AACTACAACTCATTCTCCTA+TGG - chr2_3:28683107-28683126 Msa0286860:CDS 35.0%
CCAAAGCTGTTCTATAATCA+GGG + chr2_3:28683170-28683189 None:intergenic 35.0%
TCCAAAGCTGTTCTATAATC+AGG + chr2_3:28683171-28683190 None:intergenic 35.0%
TGCATAATATATGCAAGGTC+CGG - chr2_3:28683356-28683375 Msa0286860:intron 35.0%
!!! GGTATTCTTTTTTTTGGTGG+TGG + chr2_3:28683396-28683415 None:intergenic 35.0%
ATTATTGTGCAGCTATTACC+TGG - chr2_3:28683708-28683727 Msa0286860:intron 35.0%
TTATTGTGCAGCTATTACCT+GGG - chr2_3:28683709-28683728 Msa0286860:intron 35.0%
AAAAAGAGCAAAGTCGACAA+TGG - chr2_3:28683772-28683791 Msa0286860:intron 35.0%
ATTTCAGAGTTTACAACCCA+TGG + chr2_3:28683891-28683910 None:intergenic 35.0%
! CTATCATTGACTGATGCTAT+TGG - chr2_3:28683996-28684015 Msa0286860:CDS 35.0%
!! TGTTTCTGGCACAAAAACAA+TGG + chr2_3:28684066-28684085 None:intergenic 35.0%
!! TGTTTTTGTGCCAGAAACAA+AGG - chr2_3:28684067-28684086 Msa0286860:CDS 35.0%
! GTTTTTGTGCCAGAAACAAA+GGG - chr2_3:28684068-28684087 Msa0286860:CDS 35.0%
TGAAGAGGTTGAGAAAATGT+TGG - chr2_3:28684100-28684119 Msa0286860:CDS 35.0%
AGAGGTTTATATGCAGGAGT+AGG - chr2_3:28682835-28682854 Msa0286860:CDS 40.0%
!! TGTTGATTCCGACGAATTGT+TGG + chr2_3:28682873-28682892 None:intergenic 40.0%
AAAACCAGCTAGTTGAACGA+TGG + chr2_3:28682917-28682936 None:intergenic 40.0%
ACAGCTGGAATCAATGCATT+TGG - chr2_3:28682973-28682992 Msa0286860:CDS 40.0%
CAGCAAAAGTTTCTTCCTTC+CGG + chr2_3:28683031-28683050 None:intergenic 40.0%
CCCTGATTATAGAACAGCTT+TGG - chr2_3:28683167-28683186 Msa0286860:CDS 40.0%
GGATTGCATGAAATGCCTTA+AGG - chr2_3:28683203-28683222 Msa0286860:CDS 40.0%
GAAGCACAAAATCCACATTC+AGG + chr2_3:28683234-28683253 None:intergenic 40.0%
TTGTGCTTCTGCTGGTAATA+AGG - chr2_3:28683245-28683264 Msa0286860:intron 40.0%
GCATAATATATGCAAGGTCC+GGG - chr2_3:28683357-28683376 Msa0286860:intron 40.0%
CATAATATATGCAAGGTCCG+GGG - chr2_3:28683358-28683377 Msa0286860:intron 40.0%
TATTGTGCAGCTATTACCTG+GGG - chr2_3:28683710-28683729 Msa0286860:intron 40.0%
TTTGATATTAGACATGCCCC+AGG + chr2_3:28683729-28683748 None:intergenic 40.0%
AGAGGATGTCCAAGCAAAAA+CGG - chr2_3:28683801-28683820 Msa0286860:intron 40.0%
TACATCCTATTCTTCTCACC+AGG - chr2_3:28683852-28683871 Msa0286860:CDS 40.0%
CTATTCTTCTCACCAGGAAT+GGG - chr2_3:28683858-28683877 Msa0286860:CDS 40.0%
AGATACAGAGGAATATGTGG+AGG - chr2_3:28683921-28683940 Msa0286860:CDS 40.0%
! ATAGGAAGTCCCTTTGTTTC+TGG + chr2_3:28684080-28684099 None:intergenic 40.0%
AAAGGGACTTCCTATTGAAG+AGG - chr2_3:28684085-28684104 Msa0286860:CDS 40.0%
AGAGGTTTATATGCAGGAGT+AGG - chr2_3:28682835-28682854 Msa0286860:CDS 40.0%
!! TGTTGATTCCGACGAATTGT+TGG + chr2_3:28682873-28682892 None:intergenic 40.0%
AAAACCAGCTAGTTGAACGA+TGG + chr2_3:28682917-28682936 None:intergenic 40.0%
ACAGCTGGAATCAATGCATT+TGG - chr2_3:28682973-28682992 Msa0286860:CDS 40.0%
CAGCAAAAGTTTCTTCCTTC+CGG + chr2_3:28683031-28683050 None:intergenic 40.0%
CCCTGATTATAGAACAGCTT+TGG - chr2_3:28683167-28683186 Msa0286860:CDS 40.0%
GGATTGCATGAAATGCCTTA+AGG - chr2_3:28683203-28683222 Msa0286860:CDS 40.0%
GAAGCACAAAATCCACATTC+AGG + chr2_3:28683234-28683253 None:intergenic 40.0%
TTGTGCTTCTGCTGGTAATA+AGG - chr2_3:28683245-28683264 Msa0286860:intron 40.0%
GCATAATATATGCAAGGTCC+GGG - chr2_3:28683357-28683376 Msa0286860:intron 40.0%
CATAATATATGCAAGGTCCG+GGG - chr2_3:28683358-28683377 Msa0286860:intron 40.0%
TATTGTGCAGCTATTACCTG+GGG - chr2_3:28683710-28683729 Msa0286860:intron 40.0%
TTTGATATTAGACATGCCCC+AGG + chr2_3:28683729-28683748 None:intergenic 40.0%
AGAGGATGTCCAAGCAAAAA+CGG - chr2_3:28683801-28683820 Msa0286860:intron 40.0%
TACATCCTATTCTTCTCACC+AGG - chr2_3:28683852-28683871 Msa0286860:CDS 40.0%
CTATTCTTCTCACCAGGAAT+GGG - chr2_3:28683858-28683877 Msa0286860:CDS 40.0%
AGATACAGAGGAATATGTGG+AGG - chr2_3:28683921-28683940 Msa0286860:CDS 40.0%
! ATAGGAAGTCCCTTTGTTTC+TGG + chr2_3:28684080-28684099 None:intergenic 40.0%
AAAGGGACTTCCTATTGAAG+AGG - chr2_3:28684085-28684104 Msa0286860:CDS 40.0%
GTTGGAAGACGAATCGTCTT+TGG + chr2_3:28682773-28682792 None:intergenic 45.0%
!! AGCTTTGGATGCTGCTCAAT+GGG - chr2_3:28683182-28683201 Msa0286860:CDS 45.0%
CACATTCAGGAGAAGCCTTA+AGG + chr2_3:28683221-28683240 None:intergenic 45.0%
CTTAAGGCTTCTCCTGAATG+TGG - chr2_3:28683219-28683238 Msa0286860:CDS 45.0%
! TGTGGATTTTGTGCTTCTGC+TGG - chr2_3:28683237-28683256 Msa0286860:intron 45.0%
AGTCGACAATGGTACACAAG+AGG - chr2_3:28683783-28683802 Msa0286860:intron 45.0%
GATGTCCAAGCAAAAACGGT+TGG - chr2_3:28683805-28683824 Msa0286860:intron 45.0%
! AACGGTTGGCTAGCACTAAT+CGG - chr2_3:28683819-28683838 Msa0286860:intron 45.0%
CCATTCCTGGTGAGAAGAAT+AGG + chr2_3:28683860-28683879 None:intergenic 45.0%
CCTATTCTTCTCACCAGGAA+TGG - chr2_3:28683857-28683876 Msa0286860:CDS 45.0%
AGGAATGGGAACAGTTCCAT+GGG - chr2_3:28683872-28683891 Msa0286860:CDS 45.0%
GTTGGAAGACGAATCGTCTT+TGG + chr2_3:28682773-28682792 None:intergenic 45.0%
!! AGCTTTGGATGCTGCTCAAT+GGG - chr2_3:28683182-28683201 Msa0286860:CDS 45.0%
CACATTCAGGAGAAGCCTTA+AGG + chr2_3:28683221-28683240 None:intergenic 45.0%
CTTAAGGCTTCTCCTGAATG+TGG - chr2_3:28683219-28683238 Msa0286860:CDS 45.0%
! TGTGGATTTTGTGCTTCTGC+TGG - chr2_3:28683237-28683256 Msa0286860:intron 45.0%
AGTCGACAATGGTACACAAG+AGG - chr2_3:28683783-28683802 Msa0286860:intron 45.0%
GATGTCCAAGCAAAAACGGT+TGG - chr2_3:28683805-28683824 Msa0286860:intron 45.0%
! AACGGTTGGCTAGCACTAAT+CGG - chr2_3:28683819-28683838 Msa0286860:intron 45.0%
CCATTCCTGGTGAGAAGAAT+AGG + chr2_3:28683860-28683879 None:intergenic 45.0%
CCTATTCTTCTCACCAGGAA+TGG - chr2_3:28683857-28683876 Msa0286860:CDS 45.0%
AGGAATGGGAACAGTTCCAT+GGG - chr2_3:28683872-28683891 Msa0286860:CDS 45.0%
CCAGCTAGTTGAACGATGGT+TGG + chr2_3:28682913-28682932 None:intergenic 50.0%
CCAACCATCGTTCAACTAGC+TGG - chr2_3:28682910-28682929 Msa0286860:CDS 50.0%
!! CAGCTTTGGATGCTGCTCAA+TGG - chr2_3:28683181-28683200 Msa0286860:CDS 50.0%
TGAGCTTAGCTGAGCTGGTA+AGG - chr2_3:28683329-28683348 Msa0286860:intron 50.0%
AAGGTCCGGGGTTCAAATCT+CGG - chr2_3:28683370-28683389 Msa0286860:intron 50.0%
!! GCTAGCCAACCGTTTTTGCT+TGG + chr2_3:28683813-28683832 None:intergenic 50.0%
CATGGAACTGTTCCCATTCC+TGG + chr2_3:28683873-28683892 None:intergenic 50.0%
CAGGAATGGGAACAGTTCCA+TGG - chr2_3:28683871-28683890 Msa0286860:CDS 50.0%
CCAGCTAGTTGAACGATGGT+TGG + chr2_3:28682913-28682932 None:intergenic 50.0%
CCAACCATCGTTCAACTAGC+TGG - chr2_3:28682910-28682929 Msa0286860:CDS 50.0%
!! CAGCTTTGGATGCTGCTCAA+TGG - chr2_3:28683181-28683200 Msa0286860:CDS 50.0%
TGAGCTTAGCTGAGCTGGTA+AGG - chr2_3:28683329-28683348 Msa0286860:intron 50.0%
AAGGTCCGGGGTTCAAATCT+CGG - chr2_3:28683370-28683389 Msa0286860:intron 50.0%
!! GCTAGCCAACCGTTTTTGCT+TGG + chr2_3:28683813-28683832 None:intergenic 50.0%
CATGGAACTGTTCCCATTCC+TGG + chr2_3:28683873-28683892 None:intergenic 50.0%
CAGGAATGGGAACAGTTCCA+TGG - chr2_3:28683871-28683890 Msa0286860:CDS 50.0%
CTTCTGTCACTCGTCACAGC+TGG - chr2_3:28682958-28682977 Msa0286860:CDS 55.0%
AGCTAAGCTCACGAGTCACG+CGG + chr2_3:28683320-28683339 None:intergenic 55.0%
ACTCGTGAGCTTAGCTGAGC+TGG - chr2_3:28683324-28683343 Msa0286860:intron 55.0%
CTTCTGTCACTCGTCACAGC+TGG - chr2_3:28682958-28682977 Msa0286860:CDS 55.0%
AGCTAAGCTCACGAGTCACG+CGG + chr2_3:28683320-28683339 None:intergenic 55.0%
ACTCGTGAGCTTAGCTGAGC+TGG - chr2_3:28683324-28683343 Msa0286860:intron 55.0%
GGTGGCCGAGATTTGAACCC+CGG + chr2_3:28683378-28683397 None:intergenic 60.0%
GGTGGCCGAGATTTGAACCC+CGG + chr2_3:28683378-28683397 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 28682763 28684205 28682763 ID=Msa0286860;Name=Msa0286860
chr2_3 mRNA 28682763 28684205 28682763 ID=Msa0286860-mRNA-1;Parent=Msa0286860;Name=Msa0286860-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0|0|1|1|1|2|0|283
chr2_3 exon 28683840 28684205 28683840 ID=Msa0286860-mRNA-1:exon:10146;Parent=Msa0286860-mRNA-1
chr2_3 exon 28682763 28683248 28682763 ID=Msa0286860-mRNA-1:exon:10145;Parent=Msa0286860-mRNA-1
chr2_3 CDS 28683840 28684205 28683840 ID=Msa0286860-mRNA-1:cds;Parent=Msa0286860-mRNA-1
chr2_3 CDS 28682763 28683248 28682763 ID=Msa0286860-mRNA-1:cds;Parent=Msa0286860-mRNA-1
Gene Sequence

>Msa0286860

ATGGAAACCAAAGACGATTCGTCTTCCAACAAAGTCAGCATAATCAAACTTTTGAAAACCAAAACAGTAAGAAGAGGTTTATATGCAGGAGTAGGACTACAAATCTTCCAACAATTCGTCGGAATCAACACAGTAATGTACTACAGTCCAACCATCGTTCAACTAGCTGGTTTTGCATCAAACAAAACAGCACTTCTTCTGTCACTCGTCACAGCTGGAATCAATGCATTTGGTTCGATTTTGAGCATATATTTTATTGACAAGACCGGAAGGAAGAAACTTTTGCTGTTTAGTTTGTCTGGTGTTATTGTCTCTCTTGTCGTCTTGACTGTTGTGTTCCATCAAACTACAACTCATTCTCCTATGCTATTACCTGGGGCATGTCTAATATCAAATGACACAACGGAAGATCAATGTCAAAAAGAGCAAAGTCGACAATGGTACACAAGAGGATGTCCAAGCAAAAACGGTTGGCTAGCACTAATCGGTCTTTCACTATACATCCTATTCTTCTCACCAGGAATGGGAACAGTTCCATGGGTTGTAAACTCTGAAATATATCCATTAAGATACAGAGGAATATGTGGAGGAATAGCATCAACATCAAATTGGATTTCAAATCTCATTGTTGCACAATCTTTTCTATCATTGACTGATGCTATTGGAACTTCATACACATTCATGATATTCATTTTCATCACTGTTGCTGCCATTGTTTTTGTGCCAGAAACAAAGGGACTTCCTATTGAAGAGGTTGAGAAAATGTTGGAAAGAAGAATTTTGTATTTTAAGTTTTGGCAGAGGAATTCTGACTCTGATCAAGTATTAACTCAAAAGAATGTGTCATTTTGA
Protein sequence

>Msa0286860

METKDDSSSNKVSIIKLLKTKTVRRGLYAGVGLQIFQQFVGINTVMYYSPTIVQLAGFASNKTALLLSLVTAGINAFGSILSIYFIDKTGRKKLLLFSLSGVIVSLVVLTVVFHQTTTHSPMLLPGACLISNDTTEDQCQKEQSRQWYTRGCPSKNGWLALIGLSLYILFFSPGMGTVPWVVNSEIYPLRYRGICGGIASTSNWISNLIVAQSFLSLTDAIGTSYTFMIFIFITVAAIVFVPETKGLPIEEVEKMLERRILYFKFWQRNSDSDQVLTQKNVSF*