AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0291370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0291370 MtrunA17_Chr2g0311291 95.200 250 9 1 1 250 1 247 1.45e-164 455
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0291370 AT1G79160.1 53.992 263 80 12 1 250 5 239 1.08e-71 219
Msa0291370 AT1G16500.1 45.993 287 90 13 1 250 1 259 1.55e-60 192

Find 58 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTTAATAAGTGGTTTA+AGG 0.137788 2_3:-39892553 Msa0291370:exon
CTGAAGTTAGTTCGCTTGTT+AGG 0.196285 2_3:-39894366 Msa0291370:CDS
ATAAGTGGTTTAAGGATAAT+TGG 0.226069 2_3:-39892545 Msa0291370:three_prime_UTR
AGTTCGCTTGTTAGGGTTCT+AGG 0.231093 2_3:-39894358 Msa0291370:CDS
TTCAATTGAATCACAAGAAT+TGG 0.273729 2_3:-39894224 Msa0291370:CDS
AGAATTGGATCTTGATTTAC+AGG 0.278900 2_3:-39894209 Msa0291370:CDS
CTAAATTTGATTCGTCAAAT+AGG 0.288345 2_3:+39892938 None:intergenic
AAATTCAAGTCTTGAAGTTT+TGG 0.307088 2_3:+39892991 None:intergenic
ATTCTTGAAATCGTCAATTT+CGG 0.309411 2_3:-39892797 Msa0291370:CDS
ATCCTAGGTTTCTTCATAGT+TGG 0.320748 2_3:+39892589 None:intergenic
GTTCTAGGTGGTGGTGGTGG+TGG 0.321938 2_3:-39894343 Msa0291370:CDS
AATTTAGTTGGTTTAAAGAT+AGG 0.327156 2_3:-39892453 Msa0291370:three_prime_UTR
GATTCGTCAAATAGGTTCAA+TGG 0.354020 2_3:+39892946 None:intergenic
TTTGTTCAGAAATTGGTGAT+TGG 0.359020 2_3:+39893037 None:intergenic
TTGTTCAGAAATTGGTGATT+GGG 0.387101 2_3:+39893038 None:intergenic
GGTTTCTTCATAGTTGGAAT+TGG 0.391404 2_3:+39892595 None:intergenic
GGAACATTTGATTGTGTTGA+TGG 0.395950 2_3:+39892967 None:intergenic
GAGAGAGAAAATAGAGATAG+AGG 0.402785 2_3:+39894428 None:intergenic
ATTTACAGGTTCCTAGTGGC+TGG 0.409368 2_3:-39894195 Msa0291370:CDS
TAATATTTCAATTTAATAAG+TGG 0.409427 2_3:-39892560 Msa0291370:exon
TGAAGTTAGTTCGCTTGTTA+GGG 0.430323 2_3:-39894365 Msa0291370:CDS
ACACTTTGGTAATTGCTTGT+TGG 0.436517 2_3:+39892889 None:intergenic
AGGGTTCTAGGTGGTGGTGG+TGG 0.444252 2_3:-39894346 Msa0291370:CDS
CCAATGGCTGCAGGGTGTCC+TGG 0.445132 2_3:-39892682 Msa0291370:CDS
CATTTGTTTCTCTAGTAAAA+CGG 0.455488 2_3:+39892378 None:intergenic
GAATGATAGATAAATTTAGT+TGG 0.456846 2_3:-39892465 Msa0291370:three_prime_UTR
ATTTCATCACCAATGGCTGC+AGG 0.458385 2_3:-39892691 Msa0291370:CDS
GTTAGGGTTCTAGGTGGTGG+TGG 0.461924 2_3:-39894349 Msa0291370:CDS
TTCAATTTACAAAATTGTAA+AGG 0.462670 2_3:+39892485 None:intergenic
CTTGTTAGGGTTCTAGGTGG+TGG 0.465001 2_3:-39894352 Msa0291370:CDS
AGAATAATCCTAAGTGTCCT+AGG 0.485771 2_3:-39892633 Msa0291370:CDS
GAAATATTAAGATCAATCCT+AGG 0.490407 2_3:+39892574 None:intergenic
CAGGGAAAGTTTATATTCAA+AGG 0.491847 2_3:-39893074 Msa0291370:CDS
TGTCAAGAGTGCACACACTT+TGG 0.506113 2_3:+39892875 None:intergenic
TTCTTGTGATTCAATTGAAG+AGG 0.514578 2_3:+39894228 None:intergenic
GAGTTACACCTAGGACACTT+AGG 0.522057 2_3:+39892625 None:intergenic
ATAGAGGAAGAAGATGAGAA+AGG 0.538057 2_3:+39894444 None:intergenic
ATTACAAGAGACTTGCTTGG+TGG 0.546683 2_3:-39894253 Msa0291370:CDS
CCAGGACACCCTGCAGCCAT+TGG 0.549250 2_3:+39892682 None:intergenic
GAAAATGATTTCATCACCAA+TGG 0.554457 2_3:-39892698 Msa0291370:CDS
CTGATTACAAGAGACTTGCT+TGG 0.555890 2_3:-39894256 Msa0291370:CDS
GAACATTTGATTGTGTTGAT+GGG 0.563188 2_3:+39892968 None:intergenic
TTACAAGAGACTTGCTTGGT+GGG 0.576143 2_3:-39894252 Msa0291370:CDS
TTTACAGGTTCCTAGTGGCT+GGG 0.577425 2_3:-39894194 Msa0291370:CDS
TTCTTTGAGAAACTAAGCAA+TGG 0.578329 2_3:-39894392 Msa0291370:exon
TTTCAATATACATAAAACCC+TGG 0.588667 2_3:+39892408 None:intergenic
GAAGAGCAACATCGAACGGT+AGG 0.589713 2_3:-39894310 Msa0291370:CDS
CTTGATTTACAGGTTCCTAG+TGG 0.589849 2_3:-39894199 Msa0291370:CDS
TTTGAAGGGGATATTGGCCA+GGG 0.600705 2_3:-39892425 Msa0291370:three_prime_UTR
CAAAGAAGAGCAACATCGAA+CGG 0.609358 2_3:-39894314 Msa0291370:CDS
ACGGTAGGTAATGATTCTCA+TGG 0.626022 2_3:-39894295 Msa0291370:CDS
TTTCATCACCAATGGCTGCA+GGG 0.626120 2_3:-39892690 Msa0291370:CDS
TTCCAACTATGAAGAAACCT+AGG 0.628247 2_3:-39892591 Msa0291370:CDS
TTCTCATGGTGAGAAATCAA+CGG 0.650661 2_3:-39894281 Msa0291370:CDS
AATACATAAGATAAACAACC+AGG 0.660615 2_3:+39892664 None:intergenic
TCGCTTGTTAGGGTTCTAGG+TGG 0.676853 2_3:-39894355 Msa0291370:CDS
GGTACAATAGAGTTACACCT+AGG 0.708224 2_3:+39892616 None:intergenic
GATGATGAAGAAGAATACGA+AGG 0.712070 2_3:+39892766 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAAAATTCAAATTGTTT+AGG - chr2_3:39893119-39893138 Msa0291370:intron 10.0%
!! ACTAAATAAATGAAATTAAA+TGG + chr2_3:39893458-39893477 None:intergenic 10.0%
!! TAATATTTCAATTTAATAAG+TGG - chr2_3:39894277-39894296 Msa0291370:CDS 10.0%
!!! ATTTAAAATTCAAATTGTTT+AGG - chr2_3:39893119-39893138 Msa0291370:intron 10.0%
!! ACTAAATAAATGAAATTAAA+TGG + chr2_3:39893458-39893477 None:intergenic 10.0%
!! TAATATTTCAATTTAATAAG+TGG - chr2_3:39894277-39894296 Msa0291370:CDS 10.0%
!!! GAAAATATAAATTTTCTCAT+AGG - chr2_3:39892708-39892727 Msa0291370:CDS 15.0%
!! TATGTTTACTTATGATTATT+AGG - chr2_3:39892764-39892783 Msa0291370:CDS 15.0%
!! AACTTAGATATCAAAAAATT+TGG + chr2_3:39893328-39893347 None:intergenic 15.0%
!! TATCTAAGTTAATGAAAATA+TGG - chr2_3:39893338-39893357 Msa0291370:intron 15.0%
!! AGTCTTTATGATATATTTAT+GGG + chr2_3:39893407-39893426 None:intergenic 15.0%
!! AAGTCTTTATGATATATTTA+TGG + chr2_3:39893408-39893427 None:intergenic 15.0%
!! TTCAATTTACAAAATTGTAA+AGG + chr2_3:39894355-39894374 None:intergenic 15.0%
!!! GAAAATATAAATTTTCTCAT+AGG - chr2_3:39892708-39892727 Msa0291370:CDS 15.0%
!! TATGTTTACTTATGATTATT+AGG - chr2_3:39892764-39892783 Msa0291370:CDS 15.0%
!! AACTTAGATATCAAAAAATT+TGG + chr2_3:39893328-39893347 None:intergenic 15.0%
!! TATCTAAGTTAATGAAAATA+TGG - chr2_3:39893338-39893357 Msa0291370:intron 15.0%
!! AGTCTTTATGATATATTTAT+GGG + chr2_3:39893407-39893426 None:intergenic 15.0%
!! AAGTCTTTATGATATATTTA+TGG + chr2_3:39893408-39893427 None:intergenic 15.0%
!! TTCAATTTACAAAATTGTAA+AGG + chr2_3:39894355-39894374 None:intergenic 15.0%
!!! TGAGAAAATTTATATTTTCC+CGG + chr2_3:39892707-39892726 None:intergenic 20.0%
!!! TGTGTTTGTTTTTTTTACAA+TGG - chr2_3:39892856-39892875 Msa0291370:CDS 20.0%
!!! TCTTTTTGATTTTTTACATG+AGG - chr2_3:39892959-39892978 Msa0291370:CDS 20.0%
!! TATGATATATTTATGGGTAA+GGG + chr2_3:39893401-39893420 None:intergenic 20.0%
!! TTATGATATATTTATGGGTA+AGG + chr2_3:39893402-39893421 None:intergenic 20.0%
!!! ACTGTAATAAAAGTGTAAAT+TGG + chr2_3:39893589-39893608 None:intergenic 20.0%
!!! TGATTTTTTTGTTCAGAAAT+TGG + chr2_3:39893810-39893829 None:intergenic 20.0%
!! ATTTCTGAACAAAAAAATCA+AGG - chr2_3:39893810-39893829 Msa0291370:intron 20.0%
!!! AATGATGATCTTTTTCTTAT+AGG + chr2_3:39894023-39894042 None:intergenic 20.0%
!! TCAATTTAATAAGTGGTTTA+AGG - chr2_3:39894284-39894303 Msa0291370:CDS 20.0%
!! GAATGATAGATAAATTTAGT+TGG - chr2_3:39894372-39894391 Msa0291370:CDS 20.0%
!!! AATTTAGTTGGTTTAAAGAT+AGG - chr2_3:39894384-39894403 Msa0291370:exon 20.0%
!!! TGAGAAAATTTATATTTTCC+CGG + chr2_3:39892707-39892726 None:intergenic 20.0%
!!! TGTGTTTGTTTTTTTTACAA+TGG - chr2_3:39892856-39892875 Msa0291370:CDS 20.0%
!!! TCTTTTTGATTTTTTACATG+AGG - chr2_3:39892959-39892978 Msa0291370:CDS 20.0%
!! TATGATATATTTATGGGTAA+GGG + chr2_3:39893401-39893420 None:intergenic 20.0%
!! TTATGATATATTTATGGGTA+AGG + chr2_3:39893402-39893421 None:intergenic 20.0%
!!! ACTGTAATAAAAGTGTAAAT+TGG + chr2_3:39893589-39893608 None:intergenic 20.0%
!!! TGATTTTTTTGTTCAGAAAT+TGG + chr2_3:39893810-39893829 None:intergenic 20.0%
!! ATTTCTGAACAAAAAAATCA+AGG - chr2_3:39893810-39893829 Msa0291370:intron 20.0%
!!! AATGATGATCTTTTTCTTAT+AGG + chr2_3:39894023-39894042 None:intergenic 20.0%
!! TCAATTTAATAAGTGGTTTA+AGG - chr2_3:39894284-39894303 Msa0291370:CDS 20.0%
!! GAATGATAGATAAATTTAGT+TGG - chr2_3:39894372-39894391 Msa0291370:CDS 20.0%
!!! AATTTAGTTGGTTTAAAGAT+AGG - chr2_3:39894384-39894403 Msa0291370:exon 20.0%
! TTCAATTGAATCACAAGAAT+TGG - chr2_3:39892613-39892632 Msa0291370:CDS 25.0%
!! GAGAAAATTTATATTTTCCC+GGG + chr2_3:39892706-39892725 None:intergenic 25.0%
!!! TCTTGTTATTCTCTTTTGAT+TGG - chr2_3:39893036-39893055 Msa0291370:CDS 25.0%
! AAGAGAAACATGATAATTGA+AGG - chr2_3:39893062-39893081 Msa0291370:CDS 25.0%
! AGAGAAACATGATAATTGAA+GGG - chr2_3:39893063-39893082 Msa0291370:CDS 25.0%
! TTGTTTGTGTTTCATTTGTA+TGG - chr2_3:39893179-39893198 Msa0291370:intron 25.0%
! ATGATATATTTATGGGTAAG+GGG + chr2_3:39893400-39893419 None:intergenic 25.0%
!!! TTTTGTTTTTTTATGCAGTC+AGG - chr2_3:39893744-39893763 Msa0291370:intron 25.0%
!!! TTTGTTTTTTTATGCAGTCA+GGG - chr2_3:39893745-39893764 Msa0291370:intron 25.0%
!! AAATTCAAGTCTTGAAGTTT+TGG + chr2_3:39893849-39893868 None:intergenic 25.0%
!! CTAAATTTGATTCGTCAAAT+AGG + chr2_3:39893902-39893921 None:intergenic 25.0%
! ATTCTTGAAATCGTCAATTT+CGG - chr2_3:39894040-39894059 Msa0291370:intron 25.0%
! AATACATAAGATAAACAACC+AGG + chr2_3:39894176-39894195 None:intergenic 25.0%
! GAAATATTAAGATCAATCCT+AGG + chr2_3:39894266-39894285 None:intergenic 25.0%
! ATAAGTGGTTTAAGGATAAT+TGG - chr2_3:39894292-39894311 Msa0291370:CDS 25.0%
!! TAAAGATAGGATCATTTTGA+AGG - chr2_3:39894397-39894416 Msa0291370:five_prime_UTR 25.0%
!! AAAGATAGGATCATTTTGAA+GGG - chr2_3:39894398-39894417 Msa0291370:five_prime_UTR 25.0%
! TTTCAATATACATAAAACCC+TGG + chr2_3:39894432-39894451 None:intergenic 25.0%
! TTCAATTGAATCACAAGAAT+TGG - chr2_3:39892613-39892632 Msa0291370:CDS 25.0%
!! GAGAAAATTTATATTTTCCC+GGG + chr2_3:39892706-39892725 None:intergenic 25.0%
!!! TCTTGTTATTCTCTTTTGAT+TGG - chr2_3:39893036-39893055 Msa0291370:CDS 25.0%
! AAGAGAAACATGATAATTGA+AGG - chr2_3:39893062-39893081 Msa0291370:CDS 25.0%
! AGAGAAACATGATAATTGAA+GGG - chr2_3:39893063-39893082 Msa0291370:CDS 25.0%
! TTGTTTGTGTTTCATTTGTA+TGG - chr2_3:39893179-39893198 Msa0291370:intron 25.0%
! ATGATATATTTATGGGTAAG+GGG + chr2_3:39893400-39893419 None:intergenic 25.0%
!!! TTTTGTTTTTTTATGCAGTC+AGG - chr2_3:39893744-39893763 Msa0291370:intron 25.0%
!!! TTTGTTTTTTTATGCAGTCA+GGG - chr2_3:39893745-39893764 Msa0291370:intron 25.0%
!! AAATTCAAGTCTTGAAGTTT+TGG + chr2_3:39893849-39893868 None:intergenic 25.0%
!! CTAAATTTGATTCGTCAAAT+AGG + chr2_3:39893902-39893921 None:intergenic 25.0%
! ATTCTTGAAATCGTCAATTT+CGG - chr2_3:39894040-39894059 Msa0291370:intron 25.0%
! AATACATAAGATAAACAACC+AGG + chr2_3:39894176-39894195 None:intergenic 25.0%
! GAAATATTAAGATCAATCCT+AGG + chr2_3:39894266-39894285 None:intergenic 25.0%
! ATAAGTGGTTTAAGGATAAT+TGG - chr2_3:39894292-39894311 Msa0291370:CDS 25.0%
!! TAAAGATAGGATCATTTTGA+AGG - chr2_3:39894397-39894416 Msa0291370:five_prime_UTR 25.0%
!! AAAGATAGGATCATTTTGAA+GGG - chr2_3:39894398-39894417 Msa0291370:five_prime_UTR 25.0%
! TTTCAATATACATAAAACCC+TGG + chr2_3:39894432-39894451 None:intergenic 25.0%
! TTCTTTGAGAAACTAAGCAA+TGG - chr2_3:39892445-39892464 Msa0291370:three_prime_UTR 30.0%
TTCTTGTGATTCAATTGAAG+AGG + chr2_3:39892612-39892631 None:intergenic 30.0%
AGAATTGGATCTTGATTTAC+AGG - chr2_3:39892628-39892647 Msa0291370:CDS 30.0%
AAGAAAAGGAAAATACCTGA+AGG + chr2_3:39892682-39892701 None:intergenic 30.0%
!!! CAGATTTTTTATGTGGTCTA+TGG - chr2_3:39892985-39893004 Msa0291370:CDS 30.0%
GACATAAATACAGAGTACAT+AGG + chr2_3:39893269-39893288 None:intergenic 30.0%
CAGGGAAAGTTTATATTCAA+AGG - chr2_3:39893763-39893782 Msa0291370:intron 30.0%
! TTGTTCAGAAATTGGTGATT+GGG + chr2_3:39893802-39893821 None:intergenic 30.0%
! TTTGTTCAGAAATTGGTGAT+TGG + chr2_3:39893803-39893822 None:intergenic 30.0%
! GAACATTTGATTGTGTTGAT+GGG + chr2_3:39893872-39893891 None:intergenic 30.0%
! AAGCAGATGAAACAAGTTTT+AGG + chr2_3:39893926-39893945 None:intergenic 30.0%
GAAAATGATTTCATCACCAA+TGG - chr2_3:39894139-39894158 Msa0291370:intron 30.0%
!! AAGATAGGATCATTTTGAAG+GGG - chr2_3:39894399-39894418 Msa0291370:five_prime_UTR 30.0%
! TTCTTTGAGAAACTAAGCAA+TGG - chr2_3:39892445-39892464 Msa0291370:three_prime_UTR 30.0%
TTCTTGTGATTCAATTGAAG+AGG + chr2_3:39892612-39892631 None:intergenic 30.0%
AGAATTGGATCTTGATTTAC+AGG - chr2_3:39892628-39892647 Msa0291370:CDS 30.0%
AAGAAAAGGAAAATACCTGA+AGG + chr2_3:39892682-39892701 None:intergenic 30.0%
!!! CAGATTTTTTATGTGGTCTA+TGG - chr2_3:39892985-39893004 Msa0291370:CDS 30.0%
GACATAAATACAGAGTACAT+AGG + chr2_3:39893269-39893288 None:intergenic 30.0%
CAGGGAAAGTTTATATTCAA+AGG - chr2_3:39893763-39893782 Msa0291370:intron 30.0%
! TTGTTCAGAAATTGGTGATT+GGG + chr2_3:39893802-39893821 None:intergenic 30.0%
! TTTGTTCAGAAATTGGTGAT+TGG + chr2_3:39893803-39893822 None:intergenic 30.0%
! GAACATTTGATTGTGTTGAT+GGG + chr2_3:39893872-39893891 None:intergenic 30.0%
! AAGCAGATGAAACAAGTTTT+AGG + chr2_3:39893926-39893945 None:intergenic 30.0%
GAAAATGATTTCATCACCAA+TGG - chr2_3:39894139-39894158 Msa0291370:intron 30.0%
!! AAGATAGGATCATTTTGAAG+GGG - chr2_3:39894399-39894418 Msa0291370:five_prime_UTR 30.0%
ATAGAGGAAGAAGATGAGAA+AGG + chr2_3:39892396-39892415 None:intergenic 35.0%
GAGAGAGAAAATAGAGATAG+AGG + chr2_3:39892412-39892431 None:intergenic 35.0%
! TGAAGTTAGTTCGCTTGTTA+GGG - chr2_3:39892472-39892491 Msa0291370:three_prime_UTR 35.0%
TTCTCATGGTGAGAAATCAA+CGG - chr2_3:39892556-39892575 Msa0291370:exon 35.0%
! GGTATTTTCCTTTTCTTTCC+CGG - chr2_3:39892685-39892704 Msa0291370:CDS 35.0%
! GTATTTTCCTTTTCTTTCCC+GGG - chr2_3:39892686-39892705 Msa0291370:CDS 35.0%
! ATATTTTCCCGGGAAAGAAA+AGG + chr2_3:39892696-39892715 None:intergenic 35.0%
GATGCATAGTTCTTCAATCA+TGG - chr2_3:39892884-39892903 Msa0291370:CDS 35.0%
!!! GAGGAAGCAGATTTTTTATG+TGG - chr2_3:39892978-39892997 Msa0291370:CDS 35.0%
!! TTGCGTTTTAATGTAACAGC+AGG - chr2_3:39893219-39893238 Msa0291370:intron 35.0%
ATATATTTATGGGTAAGGGG+CGG + chr2_3:39893397-39893416 None:intergenic 35.0%
AATCTACCTCATACATGTAG+AGG + chr2_3:39893650-39893669 None:intergenic 35.0%
ACATGTATGAGGTAGATTAG+TGG - chr2_3:39893652-39893671 Msa0291370:intron 35.0%
!!! ATTAGTGGTCCACTTTTTGT+AGG - chr2_3:39893667-39893686 Msa0291370:intron 35.0%
GGAACATTTGATTGTGTTGA+TGG + chr2_3:39893873-39893892 None:intergenic 35.0%
GATTCGTCAAATAGGTTCAA+TGG + chr2_3:39893894-39893913 None:intergenic 35.0%
! ACACTTTGGTAATTGCTTGT+TGG + chr2_3:39893951-39893970 None:intergenic 35.0%
GATGATGAAGAAGAATACGA+AGG + chr2_3:39894074-39894093 None:intergenic 35.0%
AGAATAATCCTAAGTGTCCT+AGG - chr2_3:39894204-39894223 Msa0291370:CDS 35.0%
GGTTTCTTCATAGTTGGAAT+TGG + chr2_3:39894245-39894264 None:intergenic 35.0%
TTCCAACTATGAAGAAACCT+AGG - chr2_3:39894246-39894265 Msa0291370:CDS 35.0%
ATCCTAGGTTTCTTCATAGT+TGG + chr2_3:39894251-39894270 None:intergenic 35.0%
!! GATCATTTTGAAGGGGATAT+TGG - chr2_3:39894406-39894425 Msa0291370:five_prime_UTR 35.0%
ATAGAGGAAGAAGATGAGAA+AGG + chr2_3:39892396-39892415 None:intergenic 35.0%
GAGAGAGAAAATAGAGATAG+AGG + chr2_3:39892412-39892431 None:intergenic 35.0%
! TGAAGTTAGTTCGCTTGTTA+GGG - chr2_3:39892472-39892491 Msa0291370:three_prime_UTR 35.0%
TTCTCATGGTGAGAAATCAA+CGG - chr2_3:39892556-39892575 Msa0291370:exon 35.0%
! GGTATTTTCCTTTTCTTTCC+CGG - chr2_3:39892685-39892704 Msa0291370:CDS 35.0%
! GTATTTTCCTTTTCTTTCCC+GGG - chr2_3:39892686-39892705 Msa0291370:CDS 35.0%
! ATATTTTCCCGGGAAAGAAA+AGG + chr2_3:39892696-39892715 None:intergenic 35.0%
GATGCATAGTTCTTCAATCA+TGG - chr2_3:39892884-39892903 Msa0291370:CDS 35.0%
!!! GAGGAAGCAGATTTTTTATG+TGG - chr2_3:39892978-39892997 Msa0291370:CDS 35.0%
!! TTGCGTTTTAATGTAACAGC+AGG - chr2_3:39893219-39893238 Msa0291370:intron 35.0%
ATATATTTATGGGTAAGGGG+CGG + chr2_3:39893397-39893416 None:intergenic 35.0%
AATCTACCTCATACATGTAG+AGG + chr2_3:39893650-39893669 None:intergenic 35.0%
ACATGTATGAGGTAGATTAG+TGG - chr2_3:39893652-39893671 Msa0291370:intron 35.0%
!!! ATTAGTGGTCCACTTTTTGT+AGG - chr2_3:39893667-39893686 Msa0291370:intron 35.0%
GGAACATTTGATTGTGTTGA+TGG + chr2_3:39893873-39893892 None:intergenic 35.0%
GATTCGTCAAATAGGTTCAA+TGG + chr2_3:39893894-39893913 None:intergenic 35.0%
! ACACTTTGGTAATTGCTTGT+TGG + chr2_3:39893951-39893970 None:intergenic 35.0%
GATGATGAAGAAGAATACGA+AGG + chr2_3:39894074-39894093 None:intergenic 35.0%
AGAATAATCCTAAGTGTCCT+AGG - chr2_3:39894204-39894223 Msa0291370:CDS 35.0%
GGTTTCTTCATAGTTGGAAT+TGG + chr2_3:39894245-39894264 None:intergenic 35.0%
TTCCAACTATGAAGAAACCT+AGG - chr2_3:39894246-39894265 Msa0291370:CDS 35.0%
ATCCTAGGTTTCTTCATAGT+TGG + chr2_3:39894251-39894270 None:intergenic 35.0%
!! GATCATTTTGAAGGGGATAT+TGG - chr2_3:39894406-39894425 Msa0291370:five_prime_UTR 35.0%
CTGAAGTTAGTTCGCTTGTT+AGG - chr2_3:39892471-39892490 Msa0291370:three_prime_UTR 40.0%
CAAAGAAGAGCAACATCGAA+CGG - chr2_3:39892523-39892542 Msa0291370:three_prime_UTR 40.0%
ACGGTAGGTAATGATTCTCA+TGG - chr2_3:39892542-39892561 Msa0291370:three_prime_UTR 40.0%
! CTGATTACAAGAGACTTGCT+TGG - chr2_3:39892581-39892600 Msa0291370:CDS 40.0%
! ATTACAAGAGACTTGCTTGG+TGG - chr2_3:39892584-39892603 Msa0291370:CDS 40.0%
! TTACAAGAGACTTGCTTGGT+GGG - chr2_3:39892585-39892604 Msa0291370:CDS 40.0%
CTTGATTTACAGGTTCCTAG+TGG - chr2_3:39892638-39892657 Msa0291370:CDS 40.0%
ATCTGATCAAAATGGCGTCA+TGG + chr2_3:39893367-39893386 None:intergenic 40.0%
GTGGAGCAATCTGATCAAAA+TGG + chr2_3:39893375-39893394 None:intergenic 40.0%
AGTTTCCGACCTACAAAAAG+TGG + chr2_3:39893679-39893698 None:intergenic 40.0%
GGTACAATAGAGTTACACCT+AGG + chr2_3:39894224-39894243 None:intergenic 40.0%
CTGAAGTTAGTTCGCTTGTT+AGG - chr2_3:39892471-39892490 Msa0291370:three_prime_UTR 40.0%
CAAAGAAGAGCAACATCGAA+CGG - chr2_3:39892523-39892542 Msa0291370:three_prime_UTR 40.0%
ACGGTAGGTAATGATTCTCA+TGG - chr2_3:39892542-39892561 Msa0291370:three_prime_UTR 40.0%
! CTGATTACAAGAGACTTGCT+TGG - chr2_3:39892581-39892600 Msa0291370:CDS 40.0%
! ATTACAAGAGACTTGCTTGG+TGG - chr2_3:39892584-39892603 Msa0291370:CDS 40.0%
! TTACAAGAGACTTGCTTGGT+GGG - chr2_3:39892585-39892604 Msa0291370:CDS 40.0%
CTTGATTTACAGGTTCCTAG+TGG - chr2_3:39892638-39892657 Msa0291370:CDS 40.0%
ATCTGATCAAAATGGCGTCA+TGG + chr2_3:39893367-39893386 None:intergenic 40.0%
GTGGAGCAATCTGATCAAAA+TGG + chr2_3:39893375-39893394 None:intergenic 40.0%
AGTTTCCGACCTACAAAAAG+TGG + chr2_3:39893679-39893698 None:intergenic 40.0%
GGTACAATAGAGTTACACCT+AGG + chr2_3:39894224-39894243 None:intergenic 40.0%
! AGTTCGCTTGTTAGGGTTCT+AGG - chr2_3:39892479-39892498 Msa0291370:three_prime_UTR 45.0%
ATTTACAGGTTCCTAGTGGC+TGG - chr2_3:39892642-39892661 Msa0291370:CDS 45.0%
TTTACAGGTTCCTAGTGGCT+GGG - chr2_3:39892643-39892662 Msa0291370:CDS 45.0%
GGAAAAACGTCTTGACCTTC+AGG - chr2_3:39892664-39892683 Msa0291370:CDS 45.0%
ACGAAAACTCGAGTAACTGC+AGG + chr2_3:39893245-39893264 None:intergenic 45.0%
CTCATGCCTCTACATGTATG+AGG - chr2_3:39893641-39893660 Msa0291370:intron 45.0%
!!! GTGGTCCACTTTTTGTAGGT+CGG - chr2_3:39893671-39893690 Msa0291370:intron 45.0%
! TGTCAAGAGTGCACACACTT+TGG + chr2_3:39893965-39893984 None:intergenic 45.0%
ATTTCATCACCAATGGCTGC+AGG - chr2_3:39894146-39894165 Msa0291370:intron 45.0%
TTTCATCACCAATGGCTGCA+GGG - chr2_3:39894147-39894166 Msa0291370:intron 45.0%
GAGTTACACCTAGGACACTT+AGG + chr2_3:39894215-39894234 None:intergenic 45.0%
!! TTTTGAAGGGGATATTGGCC+AGG - chr2_3:39894411-39894430 Msa0291370:five_prime_UTR 45.0%
TTTGAAGGGGATATTGGCCA+GGG - chr2_3:39894412-39894431 Msa0291370:five_prime_UTR 45.0%
! AGTTCGCTTGTTAGGGTTCT+AGG - chr2_3:39892479-39892498 Msa0291370:three_prime_UTR 45.0%
ATTTACAGGTTCCTAGTGGC+TGG - chr2_3:39892642-39892661 Msa0291370:CDS 45.0%
TTTACAGGTTCCTAGTGGCT+GGG - chr2_3:39892643-39892662 Msa0291370:CDS 45.0%
GGAAAAACGTCTTGACCTTC+AGG - chr2_3:39892664-39892683 Msa0291370:CDS 45.0%
ACGAAAACTCGAGTAACTGC+AGG + chr2_3:39893245-39893264 None:intergenic 45.0%
CTCATGCCTCTACATGTATG+AGG - chr2_3:39893641-39893660 Msa0291370:intron 45.0%
!!! GTGGTCCACTTTTTGTAGGT+CGG - chr2_3:39893671-39893690 Msa0291370:intron 45.0%
! TGTCAAGAGTGCACACACTT+TGG + chr2_3:39893965-39893984 None:intergenic 45.0%
ATTTCATCACCAATGGCTGC+AGG - chr2_3:39894146-39894165 Msa0291370:intron 45.0%
TTTCATCACCAATGGCTGCA+GGG - chr2_3:39894147-39894166 Msa0291370:intron 45.0%
GAGTTACACCTAGGACACTT+AGG + chr2_3:39894215-39894234 None:intergenic 45.0%
!! TTTTGAAGGGGATATTGGCC+AGG - chr2_3:39894411-39894430 Msa0291370:five_prime_UTR 45.0%
TTTGAAGGGGATATTGGCCA+GGG - chr2_3:39894412-39894431 Msa0291370:five_prime_UTR 45.0%
!! AAAAAAATTATATTAAATGT+AGG + chr2_3:39894332-39894351 None:intergenic 5.0%
!! AAAAAAATTATATTAAATGT+AGG + chr2_3:39894332-39894351 None:intergenic 5.0%
!! TCGCTTGTTAGGGTTCTAGG+TGG - chr2_3:39892482-39892501 Msa0291370:three_prime_UTR 50.0%
!! CTTGTTAGGGTTCTAGGTGG+TGG - chr2_3:39892485-39892504 Msa0291370:three_prime_UTR 50.0%
GAAGAGCAACATCGAACGGT+AGG - chr2_3:39892527-39892546 Msa0291370:three_prime_UTR 50.0%
! AGACGTTTTTCCCAGCCACT+AGG + chr2_3:39892656-39892675 None:intergenic 50.0%
TATTTATGGGTAAGGGGCGG+TGG + chr2_3:39893394-39893413 None:intergenic 50.0%
! ACATGTAGAGGCATGAGCAC+AGG + chr2_3:39893638-39893657 None:intergenic 50.0%
!! TCGCTTGTTAGGGTTCTAGG+TGG - chr2_3:39892482-39892501 Msa0291370:three_prime_UTR 50.0%
!! CTTGTTAGGGTTCTAGGTGG+TGG - chr2_3:39892485-39892504 Msa0291370:three_prime_UTR 50.0%
GAAGAGCAACATCGAACGGT+AGG - chr2_3:39892527-39892546 Msa0291370:three_prime_UTR 50.0%
! AGACGTTTTTCCCAGCCACT+AGG + chr2_3:39892656-39892675 None:intergenic 50.0%
TATTTATGGGTAAGGGGCGG+TGG + chr2_3:39893394-39893413 None:intergenic 50.0%
! ACATGTAGAGGCATGAGCAC+AGG + chr2_3:39893638-39893657 None:intergenic 50.0%
!! GTTAGGGTTCTAGGTGGTGG+TGG - chr2_3:39892488-39892507 Msa0291370:three_prime_UTR 55.0%
!! GTTAGGGTTCTAGGTGGTGG+TGG - chr2_3:39892488-39892507 Msa0291370:three_prime_UTR 55.0%
!! AGGGTTCTAGGTGGTGGTGG+TGG - chr2_3:39892491-39892510 Msa0291370:three_prime_UTR 60.0%
!! GTTCTAGGTGGTGGTGGTGG+TGG - chr2_3:39892494-39892513 Msa0291370:three_prime_UTR 60.0%
!! AGGGTTCTAGGTGGTGGTGG+TGG - chr2_3:39892491-39892510 Msa0291370:three_prime_UTR 60.0%
!! GTTCTAGGTGGTGGTGGTGG+TGG - chr2_3:39892494-39892513 Msa0291370:three_prime_UTR 60.0%
!! CTAGGTGGTGGTGGTGGTGG+TGG - chr2_3:39892497-39892516 Msa0291370:three_prime_UTR 65.0%
CCAGGACACCCTGCAGCCAT+TGG + chr2_3:39894158-39894177 None:intergenic 65.0%
! CCAATGGCTGCAGGGTGTCC+TGG - chr2_3:39894155-39894174 Msa0291370:intron 65.0%
!! CTAGGTGGTGGTGGTGGTGG+TGG - chr2_3:39892497-39892516 Msa0291370:three_prime_UTR 65.0%
CCAGGACACCCTGCAGCCAT+TGG + chr2_3:39894158-39894177 None:intergenic 65.0%
! CCAATGGCTGCAGGGTGTCC+TGG - chr2_3:39894155-39894174 Msa0291370:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 39892389 39894470 39892389 ID=Msa0291370;Name=Msa0291370
chr2_3 mRNA 39892389 39894470 39892389 ID=Msa0291370-mRNA-1;Parent=Msa0291370;Name=Msa0291370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=75|1|1|1|1|1|2|178|250
chr2_3 exon 39892389 39893097 39892389 ID=Msa0291370-mRNA-1:exon:12832;Parent=Msa0291370-mRNA-1
chr2_3 exon 39894174 39894470 39894174 ID=Msa0291370-mRNA-1:exon:12831;Parent=Msa0291370-mRNA-1
chr2_3 five_prime_UTR 39894396 39894470 39894396 ID=Msa0291370-mRNA-1:five_prime_utr;Parent=Msa0291370-mRNA-1
chr2_3 CDS 39894174 39894395 39894174 ID=Msa0291370-mRNA-1:cds;Parent=Msa0291370-mRNA-1
chr2_3 CDS 39892567 39893097 39892567 ID=Msa0291370-mRNA-1:cds;Parent=Msa0291370-mRNA-1
chr2_3 three_prime_UTR 39892389 39892566 39892389 ID=Msa0291370-mRNA-1:three_prime_utr;Parent=Msa0291370-mRNA-1
Gene Sequence

>Msa0291370

ATGGCTGCTGAAGTTAGTTCGCTTGTTAGGGTTCTAGGTGGTGGTGGTGGTGGTGGATACAAAGAAGAGCAACATCGAACGGTAGGTAATGATTCTCATGGTGAGAAATCAACGGCTCTGATTACAAGAGACTTGCTTGGTGGGTCCTCTTCAATTGAATCACAAGAATTGGATCTTGATTTACAGGTTCCTAGTGGCTGGGAAAAACGTCTTGACCTTCAGTCAGGGAAAGTTTATATTCAAAGGTGCAACACATTAACCCAATCACCAATTTCTGAACAAAAAAATCAAGGAAAACAAACAACACCAAAACTTCAAGACTTGAATTTCCCATCAACACAATCAAATGTTCCATTGAACCTATTTGACGAATCAAATTTAGACCTAAAACTTGTTTCATCTGCTTTACCAACAAGCAATTACCAAAGTGTGTGCACTCTTGACAAAGTGAAATCAGCACTTGAAAGAGCTGAAAAAGAACCTATAAGAAAAAGATCATCATTCTTGAAATCGTCAATTTCGGCAACATCGCCTTCGTATTCTTCTTCATCATCGTCGATAAGAGAACAAAATCATCAAGAAGAAGAAAGTGATCAAGAGAAAATGATTTCATCACCAATGGCTGCAGGGTGTCCTGGTTGTTTATCTTATGTATTGATTATGAAGAATAATCCTAAGTGTCCTAGGTGTAACTCTATTGTACCAATTCCAACTATGAAGAAACCTAGGATTGATCTTAATATTTCAATTTAA
Protein sequence

>Msa0291370

MAAEVSSLVRVLGGGGGGGYKEEQHRTVGNDSHGEKSTALITRDLLGGSSSIESQELDLDLQVPSGWEKRLDLQSGKVYIQRCNTLTQSPISEQKNQGKQTTPKLQDLNFPSTQSNVPLNLFDESNLDLKLVSSALPTSNYQSVCTLDKVKSALERAEKEPIRKRSSFLKSSISATSPSYSSSSSSIREQNHQEEESDQEKMISSPMAAGCPGCLSYVLIMKNNPKCPRCNSIVPIPTMKKPRIDLNISI*