AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0252550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0252550 MtrunA17_Chr2g0311291 95.200 250 9 1 1 250 1 247 6.31e-175 481
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0252550 AT1G79160.1 53.232 263 82 12 1 250 5 239 4.55e-70 215
Msa0252550 AT1G16500.1 45.296 287 92 13 1 250 1 259 5.73e-59 187

Find 58 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTTAATAAGTGGTTTA+AGG 0.137788 2_2:+43794472 Msa0252550:exon
CTGAAGTTAGTTCGCTTGTT+AGG 0.196285 2_2:+43792666 Msa0252550:CDS
ATAAGTGGTTTAAGGATAAT+TGG 0.226069 2_2:+43794480 Msa0252550:exon
AGTTCGCTTGTTAGGGTTCT+AGG 0.231093 2_2:+43792674 Msa0252550:CDS
AGAATTGGATCTTGATTTAC+AGG 0.260293 2_2:+43792823 Msa0252550:CDS
CTAAATTTGATTCGTCAAAT+AGG 0.288345 2_2:-43794087 None:intergenic
TTCAATTGAATCCCAAGAAT+TGG 0.301025 2_2:+43792808 Msa0252550:CDS
AAATTCAAGTCTTGAAGTTT+TGG 0.307088 2_2:-43794034 None:intergenic
ATCCTAGGTTTCTTCATAGT+TGG 0.320748 2_2:-43794436 None:intergenic
GTTCTAGGTGGTGGTGGTGG+TGG 0.321938 2_2:+43792689 Msa0252550:CDS
AATTTAGTTGGTTTAAAGAT+AGG 0.327156 2_2:+43794572 Msa0252550:three_prime_UTR
GTAAATCAAGATCCAATTCT+TGG 0.341520 2_2:-43792820 None:intergenic
GATTCGTCAAATAGGTTCAA+TGG 0.354020 2_2:-43794079 None:intergenic
TTTGTTCAGAAATTGGTGAT+TGG 0.359020 2_2:-43793988 None:intergenic
TTGTTCAGAAATTGGTGATT+GGG 0.387101 2_2:-43793987 None:intergenic
GGTTTCTTCATAGTTGGAAT+TGG 0.391404 2_2:-43794430 None:intergenic
GGAACATTTGATTGTGTTGA+TGG 0.395950 2_2:-43794058 None:intergenic
GAGAGAGAAAATAGAGATAG+AGG 0.402785 2_2:-43792604 None:intergenic
ATTTACAGGTTCCTAGTGGC+TGG 0.409368 2_2:+43792837 Msa0252550:CDS
TAATATTTCAATTTAATAAG+TGG 0.409427 2_2:+43794465 Msa0252550:exon
TGAAGTTAGTTCGCTTGTTA+GGG 0.430323 2_2:+43792667 Msa0252550:CDS
ACACTTTGGTAATTGCTTGT+TGG 0.436517 2_2:-43794136 None:intergenic
AGGGTTCTAGGTGGTGGTGG+TGG 0.444252 2_2:+43792686 Msa0252550:CDS
CCAATGGCTGCAGGGTGTCC+TGG 0.445132 2_2:+43794343 Msa0252550:CDS
CATTTGTTTCTCTAGTAAAA+CGG 0.455488 2_2:-43794647 None:intergenic
TAAATCAAGATCCAATTCTT+GGG 0.456351 2_2:-43792819 None:intergenic
GAATGATAGATAAATTTAGT+TGG 0.456846 2_2:+43794560 Msa0252550:three_prime_UTR
ATTTCATCACCAATGGCTGC+AGG 0.458385 2_2:+43794334 Msa0252550:CDS
GTTAGGGTTCTAGGTGGTGG+TGG 0.461924 2_2:+43792683 Msa0252550:CDS
TTCAATTTACAAAATTGTAA+AGG 0.462670 2_2:-43794540 None:intergenic
CTTGTTAGGGTTCTAGGTGG+TGG 0.465001 2_2:+43792680 Msa0252550:CDS
AGAATAATCCTAAGTGTCCT+AGG 0.485771 2_2:+43794392 Msa0252550:CDS
GAAATATTAAGATCAATCCT+AGG 0.490407 2_2:-43794451 None:intergenic
TGTCAAGAGTGCACACACTT+TGG 0.506113 2_2:-43794150 None:intergenic
CCAGGACACCCTGCAGCCAT+TGG 0.515852 2_2:-43794343 None:intergenic
GAGTTACACCTAGGACACTT+AGG 0.522057 2_2:-43794400 None:intergenic
TTCTTGGGATTCAATTGAAG+AGG 0.536073 2_2:-43792804 None:intergenic
ATAGAGGAAGAAGATGAGAA+AGG 0.538057 2_2:-43792588 None:intergenic
ATTACAAGAGACTTGCTTGG+TGG 0.546683 2_2:+43792779 Msa0252550:CDS
GAAAATGATTTCATCACCAA+TGG 0.554457 2_2:+43794327 Msa0252550:CDS
CTGATTACAAGAGACTTGCT+TGG 0.555890 2_2:+43792776 Msa0252550:CDS
GAACATTTGATTGTGTTGAT+GGG 0.563188 2_2:-43794057 None:intergenic
TTACAAGAGACTTGCTTGGT+GGG 0.576143 2_2:+43792780 Msa0252550:CDS
TTTACAGGTTCCTAGTGGCT+GGG 0.577425 2_2:+43792838 Msa0252550:CDS
TTCTTTGAGAAACTAAGCAA+TGG 0.578329 2_2:+43792640 Msa0252550:exon
TTTCAATATACATAAAACCC+TGG 0.588667 2_2:-43794617 None:intergenic
GAAGAGCAACATCGAACGGT+AGG 0.589713 2_2:+43792722 Msa0252550:CDS
CTTGATTTACAGGTTCCTAG+TGG 0.589849 2_2:+43792833 Msa0252550:CDS
GATGATGAAGAAGAATATGA+AGG 0.597387 2_2:-43794259 None:intergenic
TTTGAAGGGGATATTGGCCA+GGG 0.600705 2_2:+43794600 Msa0252550:three_prime_UTR
CAAAGAAGAGCAACATCGAA+CGG 0.609358 2_2:+43792718 Msa0252550:CDS
ACGGTAGGTAATGATTCTCA+TGG 0.626022 2_2:+43792737 Msa0252550:CDS
TTTCATCACCAATGGCTGCA+GGG 0.626120 2_2:+43794335 Msa0252550:CDS
TTCCAACTATGAAGAAACCT+AGG 0.628247 2_2:+43794434 Msa0252550:CDS
TTCTCATGGTGAGAAATCAA+CGG 0.650661 2_2:+43792751 Msa0252550:CDS
AATACATAAGATAAGCAACC+AGG 0.660615 2_2:-43794361 None:intergenic
TCGCTTGTTAGGGTTCTAGG+TGG 0.676853 2_2:+43792677 Msa0252550:CDS
GGTACAATAGAGTTACACCT+AGG 0.708224 2_2:-43794409 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAAAATTCAATTTGTTT+AGG + chr2_2:43793316-43793335 Msa0252550:intron 10.0%
!! CTAAATGAAATAAAATTAAA+TGG - chr2_2:43793672-43793691 None:intergenic 10.0%
!! TAATATTTCAATTTAATAAG+TGG + chr2_2:43794465-43794484 Msa0252550:exon 10.0%
!!! ATTTAAAATTCAATTTGTTT+AGG + chr2_2:43793316-43793335 Msa0252550:intron 10.0%
!! CTAAATGAAATAAAATTAAA+TGG - chr2_2:43793672-43793691 None:intergenic 10.0%
!! TAATATTTCAATTTAATAAG+TGG + chr2_2:43794465-43794484 Msa0252550:exon 10.0%
!!! GAAAATAAAAATTTTCTCTT+TGG + chr2_2:43792905-43792924 Msa0252550:intron 15.0%
!! TATGTTTACTTATGATTATT+AGG + chr2_2:43792961-43792980 Msa0252550:intron 15.0%
!! AACTTAGATATCAAAAAATT+TGG - chr2_2:43793542-43793561 None:intergenic 15.0%
!! TATCTAAGTTAATGAAAATA+TGG + chr2_2:43793552-43793571 Msa0252550:intron 15.0%
!! AGTCTTTATGATATATTTAT+GGG - chr2_2:43793621-43793640 None:intergenic 15.0%
!! AAGTCTTTATGATATATTTA+TGG - chr2_2:43793622-43793641 None:intergenic 15.0%
!!! ACCAATTTACATTTTTATTA+CGG + chr2_2:43793801-43793820 Msa0252550:intron 15.0%
!!! TTATTTTTTTGTTCAGAAAT+TGG - chr2_2:43793998-43794017 None:intergenic 15.0%
!! ATTTCTGAACAAAAAAATAA+AGG + chr2_2:43793998-43794017 Msa0252550:CDS 15.0%
!! TTCAATTTACAAAATTGTAA+AGG - chr2_2:43794543-43794562 None:intergenic 15.0%
!!! GAAAATAAAAATTTTCTCTT+TGG + chr2_2:43792905-43792924 Msa0252550:intron 15.0%
!! TATGTTTACTTATGATTATT+AGG + chr2_2:43792961-43792980 Msa0252550:intron 15.0%
!! AACTTAGATATCAAAAAATT+TGG - chr2_2:43793542-43793561 None:intergenic 15.0%
!! TATCTAAGTTAATGAAAATA+TGG + chr2_2:43793552-43793571 Msa0252550:intron 15.0%
!! AGTCTTTATGATATATTTAT+GGG - chr2_2:43793621-43793640 None:intergenic 15.0%
!! AAGTCTTTATGATATATTTA+TGG - chr2_2:43793622-43793641 None:intergenic 15.0%
!!! ACCAATTTACATTTTTATTA+CGG + chr2_2:43793801-43793820 Msa0252550:intron 15.0%
!!! TTATTTTTTTGTTCAGAAAT+TGG - chr2_2:43793998-43794017 None:intergenic 15.0%
!! ATTTCTGAACAAAAAAATAA+AGG + chr2_2:43793998-43794017 Msa0252550:CDS 15.0%
!! TTCAATTTACAAAATTGTAA+AGG - chr2_2:43794543-43794562 None:intergenic 15.0%
!!! AGAGAAAATTTTTATTTTCC+CGG - chr2_2:43792904-43792923 None:intergenic 20.0%
!!! TGTGTTTGTTTTTTTTACAA+TGG + chr2_2:43793053-43793072 Msa0252550:intron 20.0%
!! TATGATATATTTATGGGTAA+GGG - chr2_2:43793615-43793634 None:intergenic 20.0%
!! TTATGATATATTTATGGGTA+AGG - chr2_2:43793616-43793635 None:intergenic 20.0%
!! ACCGTAATAAAAATGTAAAT+TGG - chr2_2:43793805-43793824 None:intergenic 20.0%
!!! GTAAAACACTTATTTTTACT+TGG - chr2_2:43793896-43793915 None:intergenic 20.0%
!! AAAAAGAACCAATAAGAAAA+AGG + chr2_2:43794200-43794219 Msa0252550:CDS 20.0%
!! TCAATTTAATAAGTGGTTTA+AGG + chr2_2:43794472-43794491 Msa0252550:exon 20.0%
!! GAATGATAGATAAATTTAGT+TGG + chr2_2:43794560-43794579 Msa0252550:three_prime_UTR 20.0%
!!! AATTTAGTTGGTTTAAAGAT+AGG + chr2_2:43794572-43794591 Msa0252550:three_prime_UTR 20.0%
!!! AGAGAAAATTTTTATTTTCC+CGG - chr2_2:43792904-43792923 None:intergenic 20.0%
!!! TGTGTTTGTTTTTTTTACAA+TGG + chr2_2:43793053-43793072 Msa0252550:intron 20.0%
!! TATGATATATTTATGGGTAA+GGG - chr2_2:43793615-43793634 None:intergenic 20.0%
!! TTATGATATATTTATGGGTA+AGG - chr2_2:43793616-43793635 None:intergenic 20.0%
!! ACCGTAATAAAAATGTAAAT+TGG - chr2_2:43793805-43793824 None:intergenic 20.0%
!!! GTAAAACACTTATTTTTACT+TGG - chr2_2:43793896-43793915 None:intergenic 20.0%
!! AAAAAGAACCAATAAGAAAA+AGG + chr2_2:43794200-43794219 Msa0252550:CDS 20.0%
!! TCAATTTAATAAGTGGTTTA+AGG + chr2_2:43794472-43794491 Msa0252550:exon 20.0%
!! GAATGATAGATAAATTTAGT+TGG + chr2_2:43794560-43794579 Msa0252550:three_prime_UTR 20.0%
!!! AATTTAGTTGGTTTAAAGAT+AGG + chr2_2:43794572-43794591 Msa0252550:three_prime_UTR 20.0%
! TAAATCAAGATCCAATTCTT+GGG - chr2_2:43792822-43792841 None:intergenic 25.0%
!!! TATATTTTCCTTTTGTTTCC+CGG + chr2_2:43792882-43792901 Msa0252550:intron 25.0%
!!! GAGAAAATTTTTATTTTCCC+GGG - chr2_2:43792903-43792922 None:intergenic 25.0%
! CCTCATGTAAAAAATCAAAA+AGG - chr2_2:43793159-43793178 None:intergenic 25.0%
!!! CCTTTTTGATTTTTTACATG+AGG + chr2_2:43793156-43793175 Msa0252550:intron 25.0%
!!! CAGATTTTTTATGTTGTCTA+TGG + chr2_2:43793182-43793201 Msa0252550:intron 25.0%
!!! TTTTGTTTTGCTCTTTTGAT+TGG + chr2_2:43793233-43793252 Msa0252550:intron 25.0%
! AAGAGAAACATGATAATTGA+AGG + chr2_2:43793259-43793278 Msa0252550:intron 25.0%
! TTGTTTGTGTTTCATTTGTA+TGG + chr2_2:43793393-43793412 Msa0252550:intron 25.0%
! ATGATATATTTATGGGTAAG+GGG - chr2_2:43793614-43793633 None:intergenic 25.0%
!!! ATTTTGTTTTTTATGCAGTC+AGG + chr2_2:43793932-43793951 Msa0252550:intron 25.0%
!!! TTTTGTTTTTTATGCAGTCA+GGG + chr2_2:43793933-43793952 Msa0252550:intron 25.0%
!! AAATTCAAGTCTTGAAGTTT+TGG - chr2_2:43794037-43794056 None:intergenic 25.0%
!! CTAAATTTGATTCGTCAAAT+AGG - chr2_2:43794090-43794109 None:intergenic 25.0%
!! AATGATGACCTTTTTCTTAT+TGG - chr2_2:43794211-43794230 None:intergenic 25.0%
! GAAATATTAAGATCAATCCT+AGG - chr2_2:43794454-43794473 None:intergenic 25.0%
! ATAAGTGGTTTAAGGATAAT+TGG + chr2_2:43794480-43794499 Msa0252550:exon 25.0%
!! TAAAGATAGGATCATTTTGA+AGG + chr2_2:43794585-43794604 Msa0252550:three_prime_UTR 25.0%
!! AAAGATAGGATCATTTTGAA+GGG + chr2_2:43794586-43794605 Msa0252550:three_prime_UTR 25.0%
! TTTCAATATACATAAAACCC+TGG - chr2_2:43794620-43794639 None:intergenic 25.0%
! TAAATCAAGATCCAATTCTT+GGG - chr2_2:43792822-43792841 None:intergenic 25.0%
!!! TATATTTTCCTTTTGTTTCC+CGG + chr2_2:43792882-43792901 Msa0252550:intron 25.0%
!!! GAGAAAATTTTTATTTTCCC+GGG - chr2_2:43792903-43792922 None:intergenic 25.0%
! CCTCATGTAAAAAATCAAAA+AGG - chr2_2:43793159-43793178 None:intergenic 25.0%
!!! CCTTTTTGATTTTTTACATG+AGG + chr2_2:43793156-43793175 Msa0252550:intron 25.0%
!!! CAGATTTTTTATGTTGTCTA+TGG + chr2_2:43793182-43793201 Msa0252550:intron 25.0%
!!! TTTTGTTTTGCTCTTTTGAT+TGG + chr2_2:43793233-43793252 Msa0252550:intron 25.0%
! AAGAGAAACATGATAATTGA+AGG + chr2_2:43793259-43793278 Msa0252550:intron 25.0%
! TTGTTTGTGTTTCATTTGTA+TGG + chr2_2:43793393-43793412 Msa0252550:intron 25.0%
! ATGATATATTTATGGGTAAG+GGG - chr2_2:43793614-43793633 None:intergenic 25.0%
!!! ATTTTGTTTTTTATGCAGTC+AGG + chr2_2:43793932-43793951 Msa0252550:intron 25.0%
!!! TTTTGTTTTTTATGCAGTCA+GGG + chr2_2:43793933-43793952 Msa0252550:intron 25.0%
!! AAATTCAAGTCTTGAAGTTT+TGG - chr2_2:43794037-43794056 None:intergenic 25.0%
!! CTAAATTTGATTCGTCAAAT+AGG - chr2_2:43794090-43794109 None:intergenic 25.0%
!! AATGATGACCTTTTTCTTAT+TGG - chr2_2:43794211-43794230 None:intergenic 25.0%
! GAAATATTAAGATCAATCCT+AGG - chr2_2:43794454-43794473 None:intergenic 25.0%
! ATAAGTGGTTTAAGGATAAT+TGG + chr2_2:43794480-43794499 Msa0252550:exon 25.0%
!! TAAAGATAGGATCATTTTGA+AGG + chr2_2:43794585-43794604 Msa0252550:three_prime_UTR 25.0%
!! AAAGATAGGATCATTTTGAA+GGG + chr2_2:43794586-43794605 Msa0252550:three_prime_UTR 25.0%
! TTTCAATATACATAAAACCC+TGG - chr2_2:43794620-43794639 None:intergenic 25.0%
! TTCTTTGAGAAACTAAGCAA+TGG + chr2_2:43792640-43792659 Msa0252550:exon 30.0%
TTCAATTGAATCCCAAGAAT+TGG + chr2_2:43792808-43792827 Msa0252550:CDS 30.0%
GTAAATCAAGATCCAATTCT+TGG - chr2_2:43792823-43792842 None:intergenic 30.0%
AGAATTGGATCTTGATTTAC+AGG + chr2_2:43792823-43792842 Msa0252550:CDS 30.0%
CAAAAGGAAAATATACCTGA+AGG - chr2_2:43792877-43792896 None:intergenic 30.0%
!! ATATTTTCCTTTTGTTTCCC+GGG + chr2_2:43792883-43792902 Msa0252550:intron 30.0%
!! TTGCATTTTAATGTAACAGC+AGG + chr2_2:43793433-43793452 Msa0252550:intron 30.0%
GACATAAATACAGAGTACAT+AGG - chr2_2:43793483-43793502 None:intergenic 30.0%
TAATCTTTGCTTATGCATAG+AGG - chr2_2:43793868-43793887 None:intergenic 30.0%
! TTGTTCAGAAATTGGTGATT+GGG - chr2_2:43793990-43794009 None:intergenic 30.0%
! TTTGTTCAGAAATTGGTGAT+TGG - chr2_2:43793991-43794010 None:intergenic 30.0%
! GAACATTTGATTGTGTTGAT+GGG - chr2_2:43794060-43794079 None:intergenic 30.0%
! AAGCAGATGAAACAAGTTTT+AGG - chr2_2:43794114-43794133 None:intergenic 30.0%
GATGATGAAGAAGAATATGA+AGG - chr2_2:43794262-43794281 None:intergenic 30.0%
GAAAATGATTTCATCACCAA+TGG + chr2_2:43794327-43794346 Msa0252550:CDS 30.0%
AATACATAAGATAAGCAACC+AGG - chr2_2:43794364-43794383 None:intergenic 30.0%
!! AAGATAGGATCATTTTGAAG+GGG + chr2_2:43794587-43794606 Msa0252550:three_prime_UTR 30.0%
! TTCTTTGAGAAACTAAGCAA+TGG + chr2_2:43792640-43792659 Msa0252550:exon 30.0%
TTCAATTGAATCCCAAGAAT+TGG + chr2_2:43792808-43792827 Msa0252550:CDS 30.0%
GTAAATCAAGATCCAATTCT+TGG - chr2_2:43792823-43792842 None:intergenic 30.0%
AGAATTGGATCTTGATTTAC+AGG + chr2_2:43792823-43792842 Msa0252550:CDS 30.0%
CAAAAGGAAAATATACCTGA+AGG - chr2_2:43792877-43792896 None:intergenic 30.0%
!! ATATTTTCCTTTTGTTTCCC+GGG + chr2_2:43792883-43792902 Msa0252550:intron 30.0%
!! TTGCATTTTAATGTAACAGC+AGG + chr2_2:43793433-43793452 Msa0252550:intron 30.0%
GACATAAATACAGAGTACAT+AGG - chr2_2:43793483-43793502 None:intergenic 30.0%
TAATCTTTGCTTATGCATAG+AGG - chr2_2:43793868-43793887 None:intergenic 30.0%
! TTGTTCAGAAATTGGTGATT+GGG - chr2_2:43793990-43794009 None:intergenic 30.0%
! TTTGTTCAGAAATTGGTGAT+TGG - chr2_2:43793991-43794010 None:intergenic 30.0%
! GAACATTTGATTGTGTTGAT+GGG - chr2_2:43794060-43794079 None:intergenic 30.0%
! AAGCAGATGAAACAAGTTTT+AGG - chr2_2:43794114-43794133 None:intergenic 30.0%
GATGATGAAGAAGAATATGA+AGG - chr2_2:43794262-43794281 None:intergenic 30.0%
GAAAATGATTTCATCACCAA+TGG + chr2_2:43794327-43794346 Msa0252550:CDS 30.0%
AATACATAAGATAAGCAACC+AGG - chr2_2:43794364-43794383 None:intergenic 30.0%
!! AAGATAGGATCATTTTGAAG+GGG + chr2_2:43794587-43794606 Msa0252550:three_prime_UTR 30.0%
ATAGAGGAAGAAGATGAGAA+AGG - chr2_2:43792591-43792610 None:intergenic 35.0%
GAGAGAGAAAATAGAGATAG+AGG - chr2_2:43792607-43792626 None:intergenic 35.0%
! TGAAGTTAGTTCGCTTGTTA+GGG + chr2_2:43792667-43792686 Msa0252550:CDS 35.0%
TTCTCATGGTGAGAAATCAA+CGG + chr2_2:43792751-43792770 Msa0252550:CDS 35.0%
TTCTTGGGATTCAATTGAAG+AGG - chr2_2:43792807-43792826 None:intergenic 35.0%
! TTATTTTCCCGGGAAACAAA+AGG - chr2_2:43792893-43792912 None:intergenic 35.0%
GATGCATAGTTCTTCAATCA+TGG + chr2_2:43793081-43793100 Msa0252550:intron 35.0%
ATATATTTATGGGTAAGGGG+CGG - chr2_2:43793611-43793630 None:intergenic 35.0%
GGAACATTTGATTGTGTTGA+TGG - chr2_2:43794061-43794080 None:intergenic 35.0%
GATTCGTCAAATAGGTTCAA+TGG - chr2_2:43794082-43794101 None:intergenic 35.0%
! ACACTTTGGTAATTGCTTGT+TGG - chr2_2:43794139-43794158 None:intergenic 35.0%
AGAATAATCCTAAGTGTCCT+AGG + chr2_2:43794392-43794411 Msa0252550:CDS 35.0%
GGTTTCTTCATAGTTGGAAT+TGG - chr2_2:43794433-43794452 None:intergenic 35.0%
TTCCAACTATGAAGAAACCT+AGG + chr2_2:43794434-43794453 Msa0252550:CDS 35.0%
ATCCTAGGTTTCTTCATAGT+TGG - chr2_2:43794439-43794458 None:intergenic 35.0%
!! GATCATTTTGAAGGGGATAT+TGG + chr2_2:43794594-43794613 Msa0252550:three_prime_UTR 35.0%
ATAGAGGAAGAAGATGAGAA+AGG - chr2_2:43792591-43792610 None:intergenic 35.0%
GAGAGAGAAAATAGAGATAG+AGG - chr2_2:43792607-43792626 None:intergenic 35.0%
! TGAAGTTAGTTCGCTTGTTA+GGG + chr2_2:43792667-43792686 Msa0252550:CDS 35.0%
TTCTCATGGTGAGAAATCAA+CGG + chr2_2:43792751-43792770 Msa0252550:CDS 35.0%
TTCTTGGGATTCAATTGAAG+AGG - chr2_2:43792807-43792826 None:intergenic 35.0%
! TTATTTTCCCGGGAAACAAA+AGG - chr2_2:43792893-43792912 None:intergenic 35.0%
GATGCATAGTTCTTCAATCA+TGG + chr2_2:43793081-43793100 Msa0252550:intron 35.0%
ATATATTTATGGGTAAGGGG+CGG - chr2_2:43793611-43793630 None:intergenic 35.0%
GGAACATTTGATTGTGTTGA+TGG - chr2_2:43794061-43794080 None:intergenic 35.0%
GATTCGTCAAATAGGTTCAA+TGG - chr2_2:43794082-43794101 None:intergenic 35.0%
! ACACTTTGGTAATTGCTTGT+TGG - chr2_2:43794139-43794158 None:intergenic 35.0%
AGAATAATCCTAAGTGTCCT+AGG + chr2_2:43794392-43794411 Msa0252550:CDS 35.0%
GGTTTCTTCATAGTTGGAAT+TGG - chr2_2:43794433-43794452 None:intergenic 35.0%
TTCCAACTATGAAGAAACCT+AGG + chr2_2:43794434-43794453 Msa0252550:CDS 35.0%
ATCCTAGGTTTCTTCATAGT+TGG - chr2_2:43794439-43794458 None:intergenic 35.0%
!! GATCATTTTGAAGGGGATAT+TGG + chr2_2:43794594-43794613 Msa0252550:three_prime_UTR 35.0%
CTGAAGTTAGTTCGCTTGTT+AGG + chr2_2:43792666-43792685 Msa0252550:CDS 40.0%
CAAAGAAGAGCAACATCGAA+CGG + chr2_2:43792718-43792737 Msa0252550:CDS 40.0%
ACGGTAGGTAATGATTCTCA+TGG + chr2_2:43792737-43792756 Msa0252550:CDS 40.0%
! CTGATTACAAGAGACTTGCT+TGG + chr2_2:43792776-43792795 Msa0252550:CDS 40.0%
! ATTACAAGAGACTTGCTTGG+TGG + chr2_2:43792779-43792798 Msa0252550:CDS 40.0%
! TTACAAGAGACTTGCTTGGT+GGG + chr2_2:43792780-43792799 Msa0252550:CDS 40.0%
CTTGATTTACAGGTTCCTAG+TGG + chr2_2:43792833-43792852 Msa0252550:CDS 40.0%
ATCTGATCAAAATGGCGTCA+TGG - chr2_2:43793581-43793600 None:intergenic 40.0%
GTGGAGCAATCTGATCAAAA+TGG - chr2_2:43793589-43793608 None:intergenic 40.0%
GGTACAATAGAGTTACACCT+AGG - chr2_2:43794412-43794431 None:intergenic 40.0%
CTGAAGTTAGTTCGCTTGTT+AGG + chr2_2:43792666-43792685 Msa0252550:CDS 40.0%
CAAAGAAGAGCAACATCGAA+CGG + chr2_2:43792718-43792737 Msa0252550:CDS 40.0%
ACGGTAGGTAATGATTCTCA+TGG + chr2_2:43792737-43792756 Msa0252550:CDS 40.0%
! CTGATTACAAGAGACTTGCT+TGG + chr2_2:43792776-43792795 Msa0252550:CDS 40.0%
! ATTACAAGAGACTTGCTTGG+TGG + chr2_2:43792779-43792798 Msa0252550:CDS 40.0%
! TTACAAGAGACTTGCTTGGT+GGG + chr2_2:43792780-43792799 Msa0252550:CDS 40.0%
CTTGATTTACAGGTTCCTAG+TGG + chr2_2:43792833-43792852 Msa0252550:CDS 40.0%
ATCTGATCAAAATGGCGTCA+TGG - chr2_2:43793581-43793600 None:intergenic 40.0%
GTGGAGCAATCTGATCAAAA+TGG - chr2_2:43793589-43793608 None:intergenic 40.0%
GGTACAATAGAGTTACACCT+AGG - chr2_2:43794412-43794431 None:intergenic 40.0%
! AGTTCGCTTGTTAGGGTTCT+AGG + chr2_2:43792674-43792693 Msa0252550:CDS 45.0%
ATTTACAGGTTCCTAGTGGC+TGG + chr2_2:43792837-43792856 Msa0252550:CDS 45.0%
TTTACAGGTTCCTAGTGGCT+GGG + chr2_2:43792838-43792857 Msa0252550:CDS 45.0%
GGAAAAACGTCTTGACCTTC+AGG + chr2_2:43792859-43792878 Msa0252550:CDS 45.0%
ACGAAAACTCGAGTAACTGC+AGG - chr2_2:43793459-43793478 None:intergenic 45.0%
! TGTCAAGAGTGCACACACTT+TGG - chr2_2:43794153-43794172 None:intergenic 45.0%
ATTTCATCACCAATGGCTGC+AGG + chr2_2:43794334-43794353 Msa0252550:CDS 45.0%
TTTCATCACCAATGGCTGCA+GGG + chr2_2:43794335-43794354 Msa0252550:CDS 45.0%
GAGTTACACCTAGGACACTT+AGG - chr2_2:43794403-43794422 None:intergenic 45.0%
!! TTTTGAAGGGGATATTGGCC+AGG + chr2_2:43794599-43794618 Msa0252550:three_prime_UTR 45.0%
TTTGAAGGGGATATTGGCCA+GGG + chr2_2:43794600-43794619 Msa0252550:three_prime_UTR 45.0%
! AGTTCGCTTGTTAGGGTTCT+AGG + chr2_2:43792674-43792693 Msa0252550:CDS 45.0%
ATTTACAGGTTCCTAGTGGC+TGG + chr2_2:43792837-43792856 Msa0252550:CDS 45.0%
TTTACAGGTTCCTAGTGGCT+GGG + chr2_2:43792838-43792857 Msa0252550:CDS 45.0%
GGAAAAACGTCTTGACCTTC+AGG + chr2_2:43792859-43792878 Msa0252550:CDS 45.0%
ACGAAAACTCGAGTAACTGC+AGG - chr2_2:43793459-43793478 None:intergenic 45.0%
! TGTCAAGAGTGCACACACTT+TGG - chr2_2:43794153-43794172 None:intergenic 45.0%
ATTTCATCACCAATGGCTGC+AGG + chr2_2:43794334-43794353 Msa0252550:CDS 45.0%
TTTCATCACCAATGGCTGCA+GGG + chr2_2:43794335-43794354 Msa0252550:CDS 45.0%
GAGTTACACCTAGGACACTT+AGG - chr2_2:43794403-43794422 None:intergenic 45.0%
!! TTTTGAAGGGGATATTGGCC+AGG + chr2_2:43794599-43794618 Msa0252550:three_prime_UTR 45.0%
TTTGAAGGGGATATTGGCCA+GGG + chr2_2:43794600-43794619 Msa0252550:three_prime_UTR 45.0%
!! AAAAAAATTATATTAAATGT+AGG - chr2_2:43794520-43794539 None:intergenic 5.0%
!! AAAAAAATTATATTAAATGT+AGG - chr2_2:43794520-43794539 None:intergenic 5.0%
!! TCGCTTGTTAGGGTTCTAGG+TGG + chr2_2:43792677-43792696 Msa0252550:CDS 50.0%
!! CTTGTTAGGGTTCTAGGTGG+TGG + chr2_2:43792680-43792699 Msa0252550:CDS 50.0%
GAAGAGCAACATCGAACGGT+AGG + chr2_2:43792722-43792741 Msa0252550:CDS 50.0%
! AGACGTTTTTCCCAGCCACT+AGG - chr2_2:43792851-43792870 None:intergenic 50.0%
TATTTATGGGTAAGGGGCGG+TGG - chr2_2:43793608-43793627 None:intergenic 50.0%
!! TCGCTTGTTAGGGTTCTAGG+TGG + chr2_2:43792677-43792696 Msa0252550:CDS 50.0%
!! CTTGTTAGGGTTCTAGGTGG+TGG + chr2_2:43792680-43792699 Msa0252550:CDS 50.0%
GAAGAGCAACATCGAACGGT+AGG + chr2_2:43792722-43792741 Msa0252550:CDS 50.0%
! AGACGTTTTTCCCAGCCACT+AGG - chr2_2:43792851-43792870 None:intergenic 50.0%
TATTTATGGGTAAGGGGCGG+TGG - chr2_2:43793608-43793627 None:intergenic 50.0%
!! GTTAGGGTTCTAGGTGGTGG+TGG + chr2_2:43792683-43792702 Msa0252550:CDS 55.0%
!! GTTAGGGTTCTAGGTGGTGG+TGG + chr2_2:43792683-43792702 Msa0252550:CDS 55.0%
!! AGGGTTCTAGGTGGTGGTGG+TGG + chr2_2:43792686-43792705 Msa0252550:CDS 60.0%
!! GTTCTAGGTGGTGGTGGTGG+TGG + chr2_2:43792689-43792708 Msa0252550:CDS 60.0%
!! AGGGTTCTAGGTGGTGGTGG+TGG + chr2_2:43792686-43792705 Msa0252550:CDS 60.0%
!! GTTCTAGGTGGTGGTGGTGG+TGG + chr2_2:43792689-43792708 Msa0252550:CDS 60.0%
!! CTAGGTGGTGGTGGTGGTGG+TGG + chr2_2:43792692-43792711 Msa0252550:CDS 65.0%
CCAGGACACCCTGCAGCCAT+TGG - chr2_2:43794346-43794365 None:intergenic 65.0%
! CCAATGGCTGCAGGGTGTCC+TGG + chr2_2:43794343-43794362 Msa0252550:CDS 65.0%
!! CTAGGTGGTGGTGGTGGTGG+TGG + chr2_2:43792692-43792711 Msa0252550:CDS 65.0%
CCAGGACACCCTGCAGCCAT+TGG - chr2_2:43794346-43794365 None:intergenic 65.0%
! CCAATGGCTGCAGGGTGTCC+TGG + chr2_2:43794343-43794362 Msa0252550:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 43792584 43794658 43792584 ID=Msa0252550;Name=Msa0252550
chr2_2 mRNA 43792584 43794658 43792584 ID=Msa0252550-mRNA-1;Parent=Msa0252550;Name=Msa0252550-mRNA-1;_AED=0.02;_eAED=0.02;_QI=75|1|1|1|1|1|2|178|250
chr2_2 exon 43792584 43792880 43792584 ID=Msa0252550-mRNA-1:exon:13539;Parent=Msa0252550-mRNA-1
chr2_2 exon 43793950 43794658 43793950 ID=Msa0252550-mRNA-1:exon:13540;Parent=Msa0252550-mRNA-1
chr2_2 five_prime_UTR 43792584 43792658 43792584 ID=Msa0252550-mRNA-1:five_prime_utr;Parent=Msa0252550-mRNA-1
chr2_2 CDS 43792659 43792880 43792659 ID=Msa0252550-mRNA-1:cds;Parent=Msa0252550-mRNA-1
chr2_2 CDS 43793950 43794480 43793950 ID=Msa0252550-mRNA-1:cds;Parent=Msa0252550-mRNA-1
chr2_2 three_prime_UTR 43794481 43794658 43794481 ID=Msa0252550-mRNA-1:three_prime_utr;Parent=Msa0252550-mRNA-1
Gene Sequence

>Msa0252550

ATGGCTGCTGAAGTTAGTTCGCTTGTTAGGGTTCTAGGTGGTGGTGGTGGTGGTGGATACAAAGAAGAGCAACATCGAACGGTAGGTAATGATTCTCATGGTGAGAAATCAACGGCTCTGATTACAAGAGACTTGCTTGGTGGGTCCTCTTCAATTGAATCCCAAGAATTGGATCTTGATTTACAGGTTCCTAGTGGCTGGGAAAAACGTCTTGACCTTCAGTCAGGGAAAGTTTATATTCAAAGATGCAACACATTAACCCAATCACCAATTTCTGAACAAAAAAATAAAGGAAAATCAACAACACCAAAACTTCAAGACTTGAATTTCCCATCAACACAATCAAATGTTCCATTGAACCTATTTGACGAATCAAATTTAGACCTAAAACTTGTTTCATCTGCTTTACCAACAAGCAATTACCAAAGTGTGTGCACTCTTGACAAAGTGAAATCAGCACTTGAAAGAGCTGAAAAAGAACCAATAAGAAAAAGGTCATCATTCTTGAAATCGTCGATTTCAGCAACATCGCCTTCATATTCTTCTTCATCATCGTCGATCAGAGAACAAAATCATCAAGAAGAAGAAAGTGATCAAGAGAAAATGATTTCATCACCAATGGCTGCAGGGTGTCCTGGTTGCTTATCTTATGTATTGATTATGAAGAATAATCCTAAGTGTCCTAGGTGTAACTCTATTGTACCAATTCCAACTATGAAGAAACCTAGGATTGATCTTAATATTTCAATTTAA
Protein sequence

>Msa0252550

MAAEVSSLVRVLGGGGGGGYKEEQHRTVGNDSHGEKSTALITRDLLGGSSSIESQELDLDLQVPSGWEKRLDLQSGKVYIQRCNTLTQSPISEQKNKGKSTTPKLQDLNFPSTQSNVPLNLFDESNLDLKLVSSALPTSNYQSVCTLDKVKSALERAEKEPIRKRSSFLKSSISATSPSYSSSSSSIREQNHQEEESDQEKMISSPMAAGCPGCLSYVLIMKNNPKCPRCNSIVPIPTMKKPRIDLNISI*