AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0217030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0217030 MtrunA17_Chr2g0311291 97.166 247 7 0 1 247 1 247 1.82e-178 489
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0217030 AT1G79160.1 53.846 260 82 12 1 247 5 239 2.00e-70 216
Msa0217030 AT1G16500.1 45.775 284 92 13 1 247 1 259 2.48e-59 188

Find 56 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTTAATAAGTGGTTTA+AGG 0.137788 2_1:-63293772 Msa0217030:exon
CTGAAGTTAGCTCTCTTGTT+AGG 0.215095 2_1:-63295600 Msa0217030:CDS
AGCTCTCTTGTTAGGGTTCT+AGG 0.222073 2_1:-63295592 Msa0217030:CDS
ATAAGTGGTTTAAGGATAAT+TGG 0.226069 2_1:-63293764 Msa0217030:three_prime_UTR
AGAATTGGATCTTGATTTAC+AGG 0.260293 2_1:-63295452 Msa0217030:CDS
CTAAATTTGATTCGTCAAAT+AGG 0.288345 2_1:+63294157 None:intergenic
TTCAATTGAATCCCAAGAAT+TGG 0.301025 2_1:-63295467 Msa0217030:CDS
AAATTCAAGTCTTGAAGTTT+TGG 0.307088 2_1:+63294210 None:intergenic
ATCCTAGGTTTCTTCATAGT+TGG 0.320748 2_1:+63293808 None:intergenic
AATTTAGTTGGTTTAAAGAT+AGG 0.327156 2_1:-63293672 Msa0217030:three_prime_UTR
GTAAATCAAGATCCAATTCT+TGG 0.341520 2_1:+63295455 None:intergenic
GATTCGTCAAATAGGTTCAA+TGG 0.354020 2_1:+63294165 None:intergenic
TTTGTTCAGAAATTGGTGAT+TGG 0.359020 2_1:+63294256 None:intergenic
TTGTTCAGAAATTGGTGATT+GGG 0.387101 2_1:+63294257 None:intergenic
GGTTTCTTCATAGTTGGAAT+TGG 0.391404 2_1:+63293814 None:intergenic
GGAACATTTGATTGTGTTGA+TGG 0.395950 2_1:+63294186 None:intergenic
GAGAGAGAAAATAGAGATAG+AGG 0.402785 2_1:+63295675 None:intergenic
ATTTACAGGTTCCTAGTGGC+TGG 0.409368 2_1:-63295438 Msa0217030:CDS
TAATATTTCAATTTAATAAG+TGG 0.409427 2_1:-63293779 Msa0217030:exon
TGAAGTTAGCTCTCTTGTTA+GGG 0.424505 2_1:-63295599 Msa0217030:CDS
ACACTTTGGTAATTGCTTGT+TGG 0.436517 2_1:+63294108 None:intergenic
CCAATGGCTGCAGGGTGTCC+TGG 0.445132 2_1:-63293901 Msa0217030:CDS
CATTTGTTTCTCTAGTAAAA+CGG 0.455488 2_1:+63293597 None:intergenic
TAAATCAAGATCCAATTCTT+GGG 0.456351 2_1:+63295456 None:intergenic
GAATGATAGATAAATTTAGT+TGG 0.456846 2_1:-63293684 Msa0217030:three_prime_UTR
CTTGTTAGGGTTCTAGGTGG+TGG 0.457284 2_1:-63295586 Msa0217030:CDS
ATTTCATCACCAATGGCTGC+AGG 0.458385 2_1:-63293910 Msa0217030:CDS
TTCAATTTACAAAATTGTAA+AGG 0.462670 2_1:+63293704 None:intergenic
AGAATAATCCTAAGTGTCCT+AGG 0.485771 2_1:-63293852 Msa0217030:CDS
GAAATATTAAGATCAATCCT+AGG 0.490407 2_1:+63293793 None:intergenic
GTTAGGGTTCTAGGTGGTGG+TGG 0.502792 2_1:-63295583 Msa0217030:CDS
TGTCAAGAGTGCACACACTT+TGG 0.506113 2_1:+63294094 None:intergenic
CCAGGACACCCTGCAGCCAT+TGG 0.515852 2_1:+63293901 None:intergenic
GAGTTACACCTAGGACACTT+AGG 0.522057 2_1:+63293844 None:intergenic
TTCTTGGGATTCAATTGAAG+AGG 0.536073 2_1:+63295471 None:intergenic
ATAGAGGAAGAAGATGAGAA+AGG 0.538057 2_1:+63295691 None:intergenic
ATTACAAGAGACTTGCTTGG+TGG 0.546683 2_1:-63295496 Msa0217030:CDS
GAAAATGATTTCATCACCAA+TGG 0.554457 2_1:-63293917 Msa0217030:CDS
CTGATTACAAGAGACTTGCT+TGG 0.555890 2_1:-63295499 Msa0217030:CDS
GAACATTTGATTGTGTTGAT+GGG 0.563188 2_1:+63294187 None:intergenic
TTACAAGAGACTTGCTTGGT+GGG 0.576143 2_1:-63295495 Msa0217030:CDS
TTTACAGGTTCCTAGTGGCT+GGG 0.577425 2_1:-63295437 Msa0217030:CDS
TTTCAATATACATAAAACCC+TGG 0.588667 2_1:+63293627 None:intergenic
GAAGAGCAACATCGAACGGT+AGG 0.589713 2_1:-63295553 Msa0217030:CDS
CTTGATTTACAGGTTCCTAG+TGG 0.589849 2_1:-63295442 Msa0217030:CDS
TTCTTGGAGAAACTAAGCAA+TGG 0.590667 2_1:-63295626 Msa0217030:exon
GATGATGAAGAAGAATATGA+AGG 0.597387 2_1:+63293985 None:intergenic
TTTGAAGGGGATATTGGCCA+GGG 0.600705 2_1:-63293644 Msa0217030:three_prime_UTR
CAAAGAAGAGCAACATCGAA+CGG 0.609358 2_1:-63295557 Msa0217030:CDS
ACGGTAGGTAATGATTCTCA+TGG 0.626022 2_1:-63295538 Msa0217030:CDS
TTTCATCACCAATGGCTGCA+GGG 0.626120 2_1:-63293909 Msa0217030:CDS
TTCCAACTATGAAGAAACCT+AGG 0.628247 2_1:-63293810 Msa0217030:CDS
TTCTCATGGTGAGAAATCAA+CGG 0.650661 2_1:-63295524 Msa0217030:CDS
AATACATAAGATAAGCAACC+AGG 0.660615 2_1:+63293883 None:intergenic
TCTCTTGTTAGGGTTCTAGG+TGG 0.667514 2_1:-63295589 Msa0217030:CDS
GGTACAATAGAGTTACACCT+AGG 0.708224 2_1:+63293835 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAAAATTCAATTTGTTT+AGG - chr2_1:63294373-63294392 Msa0217030:intron 10.0%
!! ACTAAATAAATAAACTTAAA+TGG + chr2_1:63294731-63294750 None:intergenic 10.0%
!! TAATATTTCAATTTAATAAG+TGG - chr2_1:63295524-63295543 Msa0217030:CDS 10.0%
!!! ATTTAAAATTCAATTTGTTT+AGG - chr2_1:63294373-63294392 Msa0217030:intron 10.0%
!! ACTAAATAAATAAACTTAAA+TGG + chr2_1:63294731-63294750 None:intergenic 10.0%
!! TAATATTTCAATTTAATAAG+TGG - chr2_1:63295524-63295543 Msa0217030:CDS 10.0%
!!! AAATTTCACTTTTTTGTTTA+TGG - chr2_1:63294008-63294027 Msa0217030:CDS 15.0%
!!! AATTTCACTTTTTTGTTTAT+GGG - chr2_1:63294009-63294028 Msa0217030:CDS 15.0%
!! AATAGAAACATAATAATTGA+AGG - chr2_1:63294312-63294331 Msa0217030:intron 15.0%
!! ATAGAAACATAATAATTGAA+GGG - chr2_1:63294313-63294332 Msa0217030:intron 15.0%
!! AACTTAGATATCAAAAAATT+TGG + chr2_1:63294601-63294620 None:intergenic 15.0%
!! TATCTAAGTTAATGAAAATA+TGG - chr2_1:63294611-63294630 Msa0217030:intron 15.0%
!! AGTCTTTATGATATATTTAT+GGG + chr2_1:63294680-63294699 None:intergenic 15.0%
!! AAGTCTTTATGATATATTTA+TGG + chr2_1:63294681-63294700 None:intergenic 15.0%
!!! ACCAATTTACATTTTTATTA+CGG - chr2_1:63294858-63294877 Msa0217030:intron 15.0%
!!! AAAACACTTATTTTTTTACT+TGG + chr2_1:63294953-63294972 None:intergenic 15.0%
!! TTCAATTTACAAAATTGTAA+AGG + chr2_1:63295602-63295621 None:intergenic 15.0%
!!! AAATTTCACTTTTTTGTTTA+TGG - chr2_1:63294008-63294027 Msa0217030:CDS 15.0%
!!! AATTTCACTTTTTTGTTTAT+GGG - chr2_1:63294009-63294028 Msa0217030:CDS 15.0%
!! AATAGAAACATAATAATTGA+AGG - chr2_1:63294312-63294331 Msa0217030:intron 15.0%
!! ATAGAAACATAATAATTGAA+GGG - chr2_1:63294313-63294332 Msa0217030:intron 15.0%
!! AACTTAGATATCAAAAAATT+TGG + chr2_1:63294601-63294620 None:intergenic 15.0%
!! TATCTAAGTTAATGAAAATA+TGG - chr2_1:63294611-63294630 Msa0217030:intron 15.0%
!! AGTCTTTATGATATATTTAT+GGG + chr2_1:63294680-63294699 None:intergenic 15.0%
!! AAGTCTTTATGATATATTTA+TGG + chr2_1:63294681-63294700 None:intergenic 15.0%
!!! ACCAATTTACATTTTTATTA+CGG - chr2_1:63294858-63294877 Msa0217030:intron 15.0%
!!! AAAACACTTATTTTTTTACT+TGG + chr2_1:63294953-63294972 None:intergenic 15.0%
!! TTCAATTTACAAAATTGTAA+AGG + chr2_1:63295602-63295621 None:intergenic 15.0%
!!! ATTTTCTTTGAGAATTTTCT+TGG - chr2_1:63293661-63293680 Msa0217030:three_prime_UTR 20.0%
!!! AGAGAAAATTTATATTTTCC+CGG + chr2_1:63293930-63293949 None:intergenic 20.0%
!!! TGTTTGTGTTTTTTTTACAA+TGG - chr2_1:63294106-63294125 Msa0217030:CDS 20.0%
!!! TCTTTTTGATTTTTTACATG+AGG - chr2_1:63294209-63294228 Msa0217030:CDS 20.0%
!! TATGATATATTTATGGGTAA+GGG + chr2_1:63294674-63294693 None:intergenic 20.0%
!! TTATGATATATTTATGGGTA+AGG + chr2_1:63294675-63294694 None:intergenic 20.0%
!! ACCGTAATAAAAATGTAAAT+TGG + chr2_1:63294862-63294881 None:intergenic 20.0%
!!! TGATTTTTTTGTTCAGAAAT+TGG + chr2_1:63295057-63295076 None:intergenic 20.0%
!! ATTTCTGAACAAAAAAATCA+AGG - chr2_1:63295057-63295076 Msa0217030:intron 20.0%
!!! AATGATGATCTTTTTCTTAT+TGG + chr2_1:63295270-63295289 None:intergenic 20.0%
!! TCAATTTAATAAGTGGTTTA+AGG - chr2_1:63295531-63295550 Msa0217030:CDS 20.0%
!! GAATGATAGATAAATTTAGT+TGG - chr2_1:63295619-63295638 Msa0217030:exon 20.0%
!!! AATTTAGTTGGTTTAAAGAT+AGG - chr2_1:63295631-63295650 Msa0217030:five_prime_UTR 20.0%
!!! ATTTTCTTTGAGAATTTTCT+TGG - chr2_1:63293661-63293680 Msa0217030:three_prime_UTR 20.0%
!!! AGAGAAAATTTATATTTTCC+CGG + chr2_1:63293930-63293949 None:intergenic 20.0%
!!! TGTTTGTGTTTTTTTTACAA+TGG - chr2_1:63294106-63294125 Msa0217030:CDS 20.0%
!!! TCTTTTTGATTTTTTACATG+AGG - chr2_1:63294209-63294228 Msa0217030:CDS 20.0%
!! TATGATATATTTATGGGTAA+GGG + chr2_1:63294674-63294693 None:intergenic 20.0%
!! TTATGATATATTTATGGGTA+AGG + chr2_1:63294675-63294694 None:intergenic 20.0%
!! ACCGTAATAAAAATGTAAAT+TGG + chr2_1:63294862-63294881 None:intergenic 20.0%
!!! TGATTTTTTTGTTCAGAAAT+TGG + chr2_1:63295057-63295076 None:intergenic 20.0%
!! ATTTCTGAACAAAAAAATCA+AGG - chr2_1:63295057-63295076 Msa0217030:intron 20.0%
!!! AATGATGATCTTTTTCTTAT+TGG + chr2_1:63295270-63295289 None:intergenic 20.0%
!! TCAATTTAATAAGTGGTTTA+AGG - chr2_1:63295531-63295550 Msa0217030:CDS 20.0%
!! GAATGATAGATAAATTTAGT+TGG - chr2_1:63295619-63295638 Msa0217030:exon 20.0%
!!! AATTTAGTTGGTTTAAAGAT+AGG - chr2_1:63295631-63295650 Msa0217030:five_prime_UTR 20.0%
! TAAATCAAGATCCAATTCTT+GGG + chr2_1:63293850-63293869 None:intergenic 25.0%
!! GAGAAAATTTATATTTTCCC+GGG + chr2_1:63293929-63293948 None:intergenic 25.0%
! AAGTGTACACTATGATTAAT+AGG - chr2_1:63293980-63293999 Msa0217030:CDS 25.0%
!!! TTTTGTTTTGCTCTTTTGAT+TGG - chr2_1:63294286-63294305 Msa0217030:CDS 25.0%
! TTGTTTGTGTTTCATTTGTA+TGG - chr2_1:63294450-63294469 Msa0217030:intron 25.0%
! ATGATATATTTATGGGTAAG+GGG + chr2_1:63294673-63294692 None:intergenic 25.0%
!!! ATTTTGTTTTTTATGCAGTC+AGG - chr2_1:63294991-63295010 Msa0217030:intron 25.0%
!!! TTTTGTTTTTTATGCAGTCA+GGG - chr2_1:63294992-63295011 Msa0217030:intron 25.0%
!! AAATTCAAGTCTTGAAGTTT+TGG + chr2_1:63295096-63295115 None:intergenic 25.0%
!! CTAAATTTGATTCGTCAAAT+AGG + chr2_1:63295149-63295168 None:intergenic 25.0%
! GAAATATTAAGATCAATCCT+AGG + chr2_1:63295513-63295532 None:intergenic 25.0%
! ATAAGTGGTTTAAGGATAAT+TGG - chr2_1:63295539-63295558 Msa0217030:CDS 25.0%
!! TAAAGATAGGATCATTTTGA+AGG - chr2_1:63295644-63295663 Msa0217030:five_prime_UTR 25.0%
!! AAAGATAGGATCATTTTGAA+GGG - chr2_1:63295645-63295664 Msa0217030:five_prime_UTR 25.0%
! TTTCAATATACATAAAACCC+TGG + chr2_1:63295679-63295698 None:intergenic 25.0%
! TAAATCAAGATCCAATTCTT+GGG + chr2_1:63293850-63293869 None:intergenic 25.0%
!! GAGAAAATTTATATTTTCCC+GGG + chr2_1:63293929-63293948 None:intergenic 25.0%
! AAGTGTACACTATGATTAAT+AGG - chr2_1:63293980-63293999 Msa0217030:CDS 25.0%
!!! TTTTGTTTTGCTCTTTTGAT+TGG - chr2_1:63294286-63294305 Msa0217030:CDS 25.0%
! TTGTTTGTGTTTCATTTGTA+TGG - chr2_1:63294450-63294469 Msa0217030:intron 25.0%
! ATGATATATTTATGGGTAAG+GGG + chr2_1:63294673-63294692 None:intergenic 25.0%
!!! ATTTTGTTTTTTATGCAGTC+AGG - chr2_1:63294991-63295010 Msa0217030:intron 25.0%
!!! TTTTGTTTTTTATGCAGTCA+GGG - chr2_1:63294992-63295011 Msa0217030:intron 25.0%
!! AAATTCAAGTCTTGAAGTTT+TGG + chr2_1:63295096-63295115 None:intergenic 25.0%
!! CTAAATTTGATTCGTCAAAT+AGG + chr2_1:63295149-63295168 None:intergenic 25.0%
! GAAATATTAAGATCAATCCT+AGG + chr2_1:63295513-63295532 None:intergenic 25.0%
! ATAAGTGGTTTAAGGATAAT+TGG - chr2_1:63295539-63295558 Msa0217030:CDS 25.0%
!! TAAAGATAGGATCATTTTGA+AGG - chr2_1:63295644-63295663 Msa0217030:five_prime_UTR 25.0%
!! AAAGATAGGATCATTTTGAA+GGG - chr2_1:63295645-63295664 Msa0217030:five_prime_UTR 25.0%
! TTTCAATATACATAAAACCC+TGG + chr2_1:63295679-63295698 None:intergenic 25.0%
TTCAATTGAATCCCAAGAAT+TGG - chr2_1:63293836-63293855 Msa0217030:CDS 30.0%
GTAAATCAAGATCCAATTCT+TGG + chr2_1:63293851-63293870 None:intergenic 30.0%
AGAATTGGATCTTGATTTAC+AGG - chr2_1:63293851-63293870 Msa0217030:CDS 30.0%
AACAAAAGGAAAATACCTGA+AGG + chr2_1:63293905-63293924 None:intergenic 30.0%
!!! TTTTTTGTTTATGGGCTATG+TGG - chr2_1:63294017-63294036 Msa0217030:CDS 30.0%
!!! CAGATTTTTTATGTGGTCTA+TGG - chr2_1:63294235-63294254 Msa0217030:CDS 30.0%
ACTTTGTTCTATCATTGCAA+AGG + chr2_1:63294419-63294438 None:intergenic 30.0%
!! TTGCATTTTAATGTAACAGC+AGG - chr2_1:63294492-63294511 Msa0217030:intron 30.0%
GACATAAATACAGAGTACAT+AGG + chr2_1:63294542-63294561 None:intergenic 30.0%
! TTGTTCAGAAATTGGTGATT+GGG + chr2_1:63295049-63295068 None:intergenic 30.0%
! TTTGTTCAGAAATTGGTGAT+TGG + chr2_1:63295050-63295069 None:intergenic 30.0%
! GAACATTTGATTGTGTTGAT+GGG + chr2_1:63295119-63295138 None:intergenic 30.0%
! AAGCAGATGAAACAAGTTTT+AGG + chr2_1:63295173-63295192 None:intergenic 30.0%
GATGATGAAGAAGAATATGA+AGG + chr2_1:63295321-63295340 None:intergenic 30.0%
GAAAATGATTTCATCACCAA+TGG - chr2_1:63295386-63295405 Msa0217030:intron 30.0%
AATACATAAGATAAGCAACC+AGG + chr2_1:63295423-63295442 None:intergenic 30.0%
!! AAGATAGGATCATTTTGAAG+GGG - chr2_1:63295646-63295665 Msa0217030:five_prime_UTR 30.0%
TTCAATTGAATCCCAAGAAT+TGG - chr2_1:63293836-63293855 Msa0217030:CDS 30.0%
GTAAATCAAGATCCAATTCT+TGG + chr2_1:63293851-63293870 None:intergenic 30.0%
AGAATTGGATCTTGATTTAC+AGG - chr2_1:63293851-63293870 Msa0217030:CDS 30.0%
AACAAAAGGAAAATACCTGA+AGG + chr2_1:63293905-63293924 None:intergenic 30.0%
!!! TTTTTTGTTTATGGGCTATG+TGG - chr2_1:63294017-63294036 Msa0217030:CDS 30.0%
!!! CAGATTTTTTATGTGGTCTA+TGG - chr2_1:63294235-63294254 Msa0217030:CDS 30.0%
ACTTTGTTCTATCATTGCAA+AGG + chr2_1:63294419-63294438 None:intergenic 30.0%
!! TTGCATTTTAATGTAACAGC+AGG - chr2_1:63294492-63294511 Msa0217030:intron 30.0%
GACATAAATACAGAGTACAT+AGG + chr2_1:63294542-63294561 None:intergenic 30.0%
! TTGTTCAGAAATTGGTGATT+GGG + chr2_1:63295049-63295068 None:intergenic 30.0%
! TTTGTTCAGAAATTGGTGAT+TGG + chr2_1:63295050-63295069 None:intergenic 30.0%
! GAACATTTGATTGTGTTGAT+GGG + chr2_1:63295119-63295138 None:intergenic 30.0%
! AAGCAGATGAAACAAGTTTT+AGG + chr2_1:63295173-63295192 None:intergenic 30.0%
GATGATGAAGAAGAATATGA+AGG + chr2_1:63295321-63295340 None:intergenic 30.0%
GAAAATGATTTCATCACCAA+TGG - chr2_1:63295386-63295405 Msa0217030:intron 30.0%
AATACATAAGATAAGCAACC+AGG + chr2_1:63295423-63295442 None:intergenic 30.0%
!! AAGATAGGATCATTTTGAAG+GGG - chr2_1:63295646-63295665 Msa0217030:five_prime_UTR 30.0%
ATAGAGGAAGAAGATGAGAA+AGG + chr2_1:63293615-63293634 None:intergenic 35.0%
GAGAGAGAAAATAGAGATAG+AGG + chr2_1:63293631-63293650 None:intergenic 35.0%
TTCTTGGAGAAACTAAGCAA+TGG - chr2_1:63293677-63293696 Msa0217030:three_prime_UTR 35.0%
! TGAAGTTAGCTCTCTTGTTA+GGG - chr2_1:63293704-63293723 Msa0217030:three_prime_UTR 35.0%
TTCTCATGGTGAGAAATCAA+CGG - chr2_1:63293779-63293798 Msa0217030:exon 35.0%
TTCTTGGGATTCAATTGAAG+AGG + chr2_1:63293835-63293854 None:intergenic 35.0%
!! GGTATTTTCCTTTTGTTTCC+CGG - chr2_1:63293908-63293927 Msa0217030:CDS 35.0%
!! GTATTTTCCTTTTGTTTCCC+GGG - chr2_1:63293909-63293928 Msa0217030:CDS 35.0%
! ATATTTTCCCGGGAAACAAA+AGG + chr2_1:63293919-63293938 None:intergenic 35.0%
GATGCATAGTTCTTCAATCA+TGG - chr2_1:63294134-63294153 Msa0217030:CDS 35.0%
!!! GAGGAAGCAGATTTTTTATG+TGG - chr2_1:63294228-63294247 Msa0217030:CDS 35.0%
ATATATTTATGGGTAAGGGG+CGG + chr2_1:63294670-63294689 None:intergenic 35.0%
! TAAGTTTTACAACGCTTAGC+CGG - chr2_1:63294889-63294908 Msa0217030:intron 35.0%
TGATCTTTGCTTATGCATAG+AGG + chr2_1:63294925-63294944 None:intergenic 35.0%
GGAACATTTGATTGTGTTGA+TGG + chr2_1:63295120-63295139 None:intergenic 35.0%
GATTCGTCAAATAGGTTCAA+TGG + chr2_1:63295141-63295160 None:intergenic 35.0%
! ACACTTTGGTAATTGCTTGT+TGG + chr2_1:63295198-63295217 None:intergenic 35.0%
AGAATAATCCTAAGTGTCCT+AGG - chr2_1:63295451-63295470 Msa0217030:CDS 35.0%
GGTTTCTTCATAGTTGGAAT+TGG + chr2_1:63295492-63295511 None:intergenic 35.0%
TTCCAACTATGAAGAAACCT+AGG - chr2_1:63295493-63295512 Msa0217030:CDS 35.0%
ATCCTAGGTTTCTTCATAGT+TGG + chr2_1:63295498-63295517 None:intergenic 35.0%
!! GATCATTTTGAAGGGGATAT+TGG - chr2_1:63295653-63295672 Msa0217030:five_prime_UTR 35.0%
ATAGAGGAAGAAGATGAGAA+AGG + chr2_1:63293615-63293634 None:intergenic 35.0%
GAGAGAGAAAATAGAGATAG+AGG + chr2_1:63293631-63293650 None:intergenic 35.0%
TTCTTGGAGAAACTAAGCAA+TGG - chr2_1:63293677-63293696 Msa0217030:three_prime_UTR 35.0%
! TGAAGTTAGCTCTCTTGTTA+GGG - chr2_1:63293704-63293723 Msa0217030:three_prime_UTR 35.0%
TTCTCATGGTGAGAAATCAA+CGG - chr2_1:63293779-63293798 Msa0217030:exon 35.0%
TTCTTGGGATTCAATTGAAG+AGG + chr2_1:63293835-63293854 None:intergenic 35.0%
!! GGTATTTTCCTTTTGTTTCC+CGG - chr2_1:63293908-63293927 Msa0217030:CDS 35.0%
!! GTATTTTCCTTTTGTTTCCC+GGG - chr2_1:63293909-63293928 Msa0217030:CDS 35.0%
! ATATTTTCCCGGGAAACAAA+AGG + chr2_1:63293919-63293938 None:intergenic 35.0%
GATGCATAGTTCTTCAATCA+TGG - chr2_1:63294134-63294153 Msa0217030:CDS 35.0%
!!! GAGGAAGCAGATTTTTTATG+TGG - chr2_1:63294228-63294247 Msa0217030:CDS 35.0%
ATATATTTATGGGTAAGGGG+CGG + chr2_1:63294670-63294689 None:intergenic 35.0%
! TAAGTTTTACAACGCTTAGC+CGG - chr2_1:63294889-63294908 Msa0217030:intron 35.0%
TGATCTTTGCTTATGCATAG+AGG + chr2_1:63294925-63294944 None:intergenic 35.0%
GGAACATTTGATTGTGTTGA+TGG + chr2_1:63295120-63295139 None:intergenic 35.0%
GATTCGTCAAATAGGTTCAA+TGG + chr2_1:63295141-63295160 None:intergenic 35.0%
! ACACTTTGGTAATTGCTTGT+TGG + chr2_1:63295198-63295217 None:intergenic 35.0%
AGAATAATCCTAAGTGTCCT+AGG - chr2_1:63295451-63295470 Msa0217030:CDS 35.0%
GGTTTCTTCATAGTTGGAAT+TGG + chr2_1:63295492-63295511 None:intergenic 35.0%
TTCCAACTATGAAGAAACCT+AGG - chr2_1:63295493-63295512 Msa0217030:CDS 35.0%
ATCCTAGGTTTCTTCATAGT+TGG + chr2_1:63295498-63295517 None:intergenic 35.0%
!! GATCATTTTGAAGGGGATAT+TGG - chr2_1:63295653-63295672 Msa0217030:five_prime_UTR 35.0%
! CTGAAGTTAGCTCTCTTGTT+AGG - chr2_1:63293703-63293722 Msa0217030:three_prime_UTR 40.0%
CAAAGAAGAGCAACATCGAA+CGG - chr2_1:63293746-63293765 Msa0217030:three_prime_UTR 40.0%
ACGGTAGGTAATGATTCTCA+TGG - chr2_1:63293765-63293784 Msa0217030:three_prime_UTR 40.0%
! CTGATTACAAGAGACTTGCT+TGG - chr2_1:63293804-63293823 Msa0217030:CDS 40.0%
! ATTACAAGAGACTTGCTTGG+TGG - chr2_1:63293807-63293826 Msa0217030:CDS 40.0%
! TTACAAGAGACTTGCTTGGT+GGG - chr2_1:63293808-63293827 Msa0217030:CDS 40.0%
CTTGATTTACAGGTTCCTAG+TGG - chr2_1:63293861-63293880 Msa0217030:CDS 40.0%
ATCTGATCAAAATGGCGTCA+TGG + chr2_1:63294640-63294659 None:intergenic 40.0%
GTGGAGCAATCTGATCAAAA+TGG + chr2_1:63294648-63294667 None:intergenic 40.0%
GGTACAATAGAGTTACACCT+AGG + chr2_1:63295471-63295490 None:intergenic 40.0%
! CTGAAGTTAGCTCTCTTGTT+AGG - chr2_1:63293703-63293722 Msa0217030:three_prime_UTR 40.0%
CAAAGAAGAGCAACATCGAA+CGG - chr2_1:63293746-63293765 Msa0217030:three_prime_UTR 40.0%
ACGGTAGGTAATGATTCTCA+TGG - chr2_1:63293765-63293784 Msa0217030:three_prime_UTR 40.0%
! CTGATTACAAGAGACTTGCT+TGG - chr2_1:63293804-63293823 Msa0217030:CDS 40.0%
! ATTACAAGAGACTTGCTTGG+TGG - chr2_1:63293807-63293826 Msa0217030:CDS 40.0%
! TTACAAGAGACTTGCTTGGT+GGG - chr2_1:63293808-63293827 Msa0217030:CDS 40.0%
CTTGATTTACAGGTTCCTAG+TGG - chr2_1:63293861-63293880 Msa0217030:CDS 40.0%
ATCTGATCAAAATGGCGTCA+TGG + chr2_1:63294640-63294659 None:intergenic 40.0%
GTGGAGCAATCTGATCAAAA+TGG + chr2_1:63294648-63294667 None:intergenic 40.0%
GGTACAATAGAGTTACACCT+AGG + chr2_1:63295471-63295490 None:intergenic 40.0%
AGCTCTCTTGTTAGGGTTCT+AGG - chr2_1:63293711-63293730 Msa0217030:three_prime_UTR 45.0%
!! TCTCTTGTTAGGGTTCTAGG+TGG - chr2_1:63293714-63293733 Msa0217030:three_prime_UTR 45.0%
ATTTACAGGTTCCTAGTGGC+TGG - chr2_1:63293865-63293884 Msa0217030:CDS 45.0%
TTTACAGGTTCCTAGTGGCT+GGG - chr2_1:63293866-63293885 Msa0217030:CDS 45.0%
GGAAAAACGTCTTGACCTTC+AGG - chr2_1:63293887-63293906 Msa0217030:CDS 45.0%
ACGAAAACTCGAGTAACTGC+AGG + chr2_1:63294518-63294537 None:intergenic 45.0%
! TGTCAAGAGTGCACACACTT+TGG + chr2_1:63295212-63295231 None:intergenic 45.0%
ATTTCATCACCAATGGCTGC+AGG - chr2_1:63295393-63295412 Msa0217030:intron 45.0%
TTTCATCACCAATGGCTGCA+GGG - chr2_1:63295394-63295413 Msa0217030:intron 45.0%
GAGTTACACCTAGGACACTT+AGG + chr2_1:63295462-63295481 None:intergenic 45.0%
!! TTTTGAAGGGGATATTGGCC+AGG - chr2_1:63295658-63295677 Msa0217030:five_prime_UTR 45.0%
TTTGAAGGGGATATTGGCCA+GGG - chr2_1:63295659-63295678 Msa0217030:five_prime_UTR 45.0%
AGCTCTCTTGTTAGGGTTCT+AGG - chr2_1:63293711-63293730 Msa0217030:three_prime_UTR 45.0%
!! TCTCTTGTTAGGGTTCTAGG+TGG - chr2_1:63293714-63293733 Msa0217030:three_prime_UTR 45.0%
ATTTACAGGTTCCTAGTGGC+TGG - chr2_1:63293865-63293884 Msa0217030:CDS 45.0%
TTTACAGGTTCCTAGTGGCT+GGG - chr2_1:63293866-63293885 Msa0217030:CDS 45.0%
GGAAAAACGTCTTGACCTTC+AGG - chr2_1:63293887-63293906 Msa0217030:CDS 45.0%
ACGAAAACTCGAGTAACTGC+AGG + chr2_1:63294518-63294537 None:intergenic 45.0%
! TGTCAAGAGTGCACACACTT+TGG + chr2_1:63295212-63295231 None:intergenic 45.0%
ATTTCATCACCAATGGCTGC+AGG - chr2_1:63295393-63295412 Msa0217030:intron 45.0%
TTTCATCACCAATGGCTGCA+GGG - chr2_1:63295394-63295413 Msa0217030:intron 45.0%
GAGTTACACCTAGGACACTT+AGG + chr2_1:63295462-63295481 None:intergenic 45.0%
!! TTTTGAAGGGGATATTGGCC+AGG - chr2_1:63295658-63295677 Msa0217030:five_prime_UTR 45.0%
TTTGAAGGGGATATTGGCCA+GGG - chr2_1:63295659-63295678 Msa0217030:five_prime_UTR 45.0%
!! AAAAAAATTATATTAAATGT+AGG + chr2_1:63295579-63295598 None:intergenic 5.0%
!! AAAAAAATTATATTAAATGT+AGG + chr2_1:63295579-63295598 None:intergenic 5.0%
!! CTTGTTAGGGTTCTAGGTGG+TGG - chr2_1:63293717-63293736 Msa0217030:three_prime_UTR 50.0%
GAAGAGCAACATCGAACGGT+AGG - chr2_1:63293750-63293769 Msa0217030:three_prime_UTR 50.0%
! AGACGTTTTTCCCAGCCACT+AGG + chr2_1:63293879-63293898 None:intergenic 50.0%
TATTTATGGGTAAGGGGCGG+TGG + chr2_1:63294667-63294686 None:intergenic 50.0%
! GCATAGAGGCATATGAGCAC+CGG + chr2_1:63294911-63294930 None:intergenic 50.0%
!! CTTGTTAGGGTTCTAGGTGG+TGG - chr2_1:63293717-63293736 Msa0217030:three_prime_UTR 50.0%
GAAGAGCAACATCGAACGGT+AGG - chr2_1:63293750-63293769 Msa0217030:three_prime_UTR 50.0%
! AGACGTTTTTCCCAGCCACT+AGG + chr2_1:63293879-63293898 None:intergenic 50.0%
TATTTATGGGTAAGGGGCGG+TGG + chr2_1:63294667-63294686 None:intergenic 50.0%
! GCATAGAGGCATATGAGCAC+CGG + chr2_1:63294911-63294930 None:intergenic 50.0%
!! GTTAGGGTTCTAGGTGGTGG+TGG - chr2_1:63293720-63293739 Msa0217030:three_prime_UTR 55.0%
!! GTTAGGGTTCTAGGTGGTGG+TGG - chr2_1:63293720-63293739 Msa0217030:three_prime_UTR 55.0%
CCAGGACACCCTGCAGCCAT+TGG + chr2_1:63295405-63295424 None:intergenic 65.0%
! CCAATGGCTGCAGGGTGTCC+TGG - chr2_1:63295402-63295421 Msa0217030:intron 65.0%
CCAGGACACCCTGCAGCCAT+TGG + chr2_1:63295405-63295424 None:intergenic 65.0%
! CCAATGGCTGCAGGGTGTCC+TGG - chr2_1:63295402-63295421 Msa0217030:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 63293608 63295717 63293608 ID=Msa0217030;Name=Msa0217030
chr2_1 mRNA 63293608 63295717 63293608 ID=Msa0217030-mRNA-1;Parent=Msa0217030;Name=Msa0217030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=88|1|1|1|1|1|2|178|247
chr2_1 exon 63293608 63294316 63293608 ID=Msa0217030-mRNA-1:exon:17634;Parent=Msa0217030-mRNA-1
chr2_1 exon 63295417 63295717 63295417 ID=Msa0217030-mRNA-1:exon:17633;Parent=Msa0217030-mRNA-1
chr2_1 five_prime_UTR 63295630 63295717 63295630 ID=Msa0217030-mRNA-1:five_prime_utr;Parent=Msa0217030-mRNA-1
chr2_1 CDS 63295417 63295629 63295417 ID=Msa0217030-mRNA-1:cds;Parent=Msa0217030-mRNA-1
chr2_1 CDS 63293786 63294316 63293786 ID=Msa0217030-mRNA-1:cds;Parent=Msa0217030-mRNA-1
chr2_1 three_prime_UTR 63293608 63293785 63293608 ID=Msa0217030-mRNA-1:three_prime_utr;Parent=Msa0217030-mRNA-1
Gene Sequence

>Msa0217030

ATGGCTACTGAAGTTAGCTCTCTTGTTAGGGTTCTAGGTGGTGGTGGATACAAAGAAGAGCAACATCGAACGGTAGGTAATGATTCTCATGGTGAGAAATCAACGGCTCTGATTACAAGAGACTTGCTTGGTGGGTCCTCTTCAATTGAATCCCAAGAATTGGATCTTGATTTACAGGTTCCTAGTGGCTGGGAAAAACGTCTTGACCTTCAGTCAGGGAAAGTTTATATTCAAAGATGCAACACATTAACCCAATCACCAATTTCTGAACAAAAAAATCAAGGAAAATCAACAACACCAAAACTTCAAGACTTGAATTTCCCATCAACACAATCAAATGTTCCATTGAACCTATTTGACGAATCAAATTTAGACCTAAAACTTGTTTCATCTGCTTTACCAACAAGCAATTACCAAAGTGTGTGCACTCTTGACAAAGTGAAATCAGCACTTGAAAGAGCTGAAAAAGAACCAATAAGAAAAAGATCATCATTCTTGAAATCGTCGATTTCAGCAACATCGCCTTCATATTCTTCTTCATCATCGTCGATCAGAGAACAAAATCATCAAGAAGAAGAAAGTGATCAAGAGAAAATGATTTCATCACCAATGGCTGCAGGGTGTCCTGGTTGCTTATCTTATGTATTGATTATGAAGAATAATCCTAAGTGTCCTAGGTGTAACTCTATTGTACCAATTCCAACTATGAAGAAACCTAGGATTGATCTTAATATTTCAATTTAA
Protein sequence

>Msa0217030

MATEVSSLVRVLGGGGYKEEQHRTVGNDSHGEKSTALITRDLLGGSSSIESQELDLDLQVPSGWEKRLDLQSGKVYIQRCNTLTQSPISEQKNQGKSTTPKLQDLNFPSTQSNVPLNLFDESNLDLKLVSSALPTSNYQSVCTLDKVKSALERAEKEPIRKRSSFLKSSISATSPSYSSSSSSIREQNHQEEESDQEKMISSPMAAGCPGCLSYVLIMKNNPKCPRCNSIVPIPTMKKPRIDLNISI*