AlfalfaGEDB | Msa0217030

M. sativa cultivar zhongmu-4 / Msa0217030

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0217030	XP_013464217.1	97.166	247	7	0	1	247	1	247	1.98e-174	489

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0217030	A0A072V8W9	97.166	247	7	0	1	247	1	247	9.44e-175	489

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017320	Msa0217030	0.824963	6.069025e-54	-8.615850e-47
Msa0042550	Msa0217030	0.808925	2.436902e-50	-8.615850e-47
Msa0043980	Msa0217030	0.800794	1.219211e-48	-8.615850e-47
Msa0065650	Msa0217030	0.808735	2.676654e-50	-8.615850e-47
Msa0124550	Msa0217030	0.833244	5.961237e-56	-8.615850e-47
Msa0126910	Msa0217030	0.800051	1.728121e-48	-8.615850e-47
Msa0138020	Msa0217030	0.832336	1.001640e-55	-8.615850e-47
Msa0142280	Msa0217030	0.801381	9.250542e-49	-8.615850e-47
Msa0142500	Msa0217030	0.832196	1.085307e-55	-8.615850e-47
Msa0171980	Msa0217030	0.803508	3.371604e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0217030	MtrunA17_Chr2g0311291	97.166	247	7	0	1	247	1	247	1.82e-178	489

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0217030	AT1G79160.1	53.846	260	82	12	1	247	5	239	2.00e-70	216
Msa0217030	AT1G16500.1	45.775	284	92	13	1	247	1	259	2.48e-59	188

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Find 56 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTCAAGTCTTGAAGTTT+TGG	0.307088	2_1:+63294210	None:intergenic
AATACATAAGATAAGCAACC+AGG	0.660615	2_1:+63293883	None:intergenic
AATTTAGTTGGTTTAAAGAT+AGG	0.327156	2_1:-63293672	Msa0217030:three_prime_UTR
ACACTTTGGTAATTGCTTGT+TGG	0.436517	2_1:+63294108	None:intergenic
ACGGTAGGTAATGATTCTCA+TGG	0.626022	2_1:-63295538	Msa0217030:CDS
AGAATAATCCTAAGTGTCCT+AGG	0.485771	2_1:-63293852	Msa0217030:CDS
AGAATTGGATCTTGATTTAC+AGG	0.260293	2_1:-63295452	Msa0217030:CDS
AGCTCTCTTGTTAGGGTTCT+AGG	0.222073	2_1:-63295592	Msa0217030:CDS
ATAAGTGGTTTAAGGATAAT+TGG	0.226069	2_1:-63293764	Msa0217030:three_prime_UTR
ATAGAGGAAGAAGATGAGAA+AGG	0.538057	2_1:+63295691	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCAATTGAATCCCAAGAAT+TGG	-	chr2_1:63293836-63293855	Msa0217030:CDS	30.0%
GTAAATCAAGATCCAATTCT+TGG	+	chr2_1:63293851-63293870	None:intergenic	30.0%
AGAATTGGATCTTGATTTAC+AGG	-	chr2_1:63293851-63293870	Msa0217030:CDS	30.0%
AACAAAAGGAAAATACCTGA+AGG	+	chr2_1:63293905-63293924	None:intergenic	30.0%
ACTTTGTTCTATCATTGCAA+AGG	+	chr2_1:63294419-63294438	None:intergenic	30.0%
GACATAAATACAGAGTACAT+AGG	+	chr2_1:63294542-63294561	None:intergenic	30.0%
GATGATGAAGAAGAATATGA+AGG	+	chr2_1:63295321-63295340	None:intergenic	30.0%
GAAAATGATTTCATCACCAA+TGG	-	chr2_1:63295386-63295405	Msa0217030:intron	30.0%
AATACATAAGATAAGCAACC+AGG	+	chr2_1:63295423-63295442	None:intergenic	30.0%
TTCAATTGAATCCCAAGAAT+TGG	-	chr2_1:63293836-63293855	Msa0217030:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	63293608	63295717	63293608	ID=Msa0217030;Name=Msa0217030
chr2_1	mRNA	63293608	63295717	63293608	ID=Msa0217030-mRNA-1;Parent=Msa0217030;Name=Msa0217030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=88\|1\|1\|1\|1\|1\|2\|178\|247
chr2_1	exon	63293608	63294316	63293608	ID=Msa0217030-mRNA-1:exon:17634;Parent=Msa0217030-mRNA-1
chr2_1	exon	63295417	63295717	63295417	ID=Msa0217030-mRNA-1:exon:17633;Parent=Msa0217030-mRNA-1
chr2_1	five_prime_UTR	63295630	63295717	63295630	ID=Msa0217030-mRNA-1:five_prime_utr;Parent=Msa0217030-mRNA-1
chr2_1	CDS	63295417	63295629	63295417	ID=Msa0217030-mRNA-1:cds;Parent=Msa0217030-mRNA-1
chr2_1	CDS	63293786	63294316	63293786	ID=Msa0217030-mRNA-1:cds;Parent=Msa0217030-mRNA-1
chr2_1	three_prime_UTR	63293608	63293785	63293608	ID=Msa0217030-mRNA-1:three_prime_utr;Parent=Msa0217030-mRNA-1

Gene Sequence

>Msa0217030

ATGGCTACTGAAGTTAGCTCTCTTGTTAGGGTTCTAGGTGGTGGTGGATACAAAGAAGAGCAACATCGAACGGTAGGTAATGATTCTCATGGTGAGAAATCAACGGCTCTGATTACAAGAGACTTGCTTGGTGGGTCCTCTTCAATTGAATCCCAAGAATTGGATCTTGATTTACAGGTTCCTAGTGGCTGGGAAAAACGTCTTGACCTTCAGTCAGGGAAAGTTTATATTCAAAGATGCAACACATTAACCCAATCACCAATTTCTGAACAAAAAAATCAAGGAAAATCAACAACACCAAAACTTCAAGACTTGAATTTCCCATCAACACAATCAAATGTTCCATTGAACCTATTTGACGAATCAAATTTAGACCTAAAACTTGTTTCATCTGCTTTACCAACAAGCAATTACCAAAGTGTGTGCACTCTTGACAAAGTGAAATCAGCACTTGAAAGAGCTGAAAAAGAACCAATAAGAAAAAGATCATCATTCTTGAAATCGTCGATTTCAGCAACATCGCCTTCATATTCTTCTTCATCATCGTCGATCAGAGAACAAAATCATCAAGAAGAAGAAAGTGATCAAGAGAAAATGATTTCATCACCAATGGCTGCAGGGTGTCCTGGTTGCTTATCTTATGTATTGATTATGAAGAATAATCCTAAGTGTCCTAGGTGTAACTCTATTGTACCAATTCCAACTATGAAGAAACCTAGGATTGATCTTAATATTTCAATTTAA

Protein sequence

>Msa0217030

MATEVSSLVRVLGGGGYKEEQHRTVGNDSHGEKSTALITRDLLGGSSSIESQELDLDLQVPSGWEKRLDLQSGKVYIQRCNTLTQSPISEQKNQGKSTTPKLQDLNFPSTQSNVPLNLFDESNLDLKLVSSALPTSNYQSVCTLDKVKSALERAEKEPIRKRSSFLKSSISATSPSYSSSSSSIREQNHQEEESDQEKMISSPMAAGCPGCLSYVLIMKNNPKCPRCNSIVPIPTMKKPRIDLNISI*