AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0292250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0292250 MtrunA17_Chr2g0312791 96.226 106 4 0 1 106 1 106 4.86e-73 216
Msa0292250 MtrunA17_Chr2g0312771 74.528 106 27 0 1 106 1 106 4.17e-57 175
Msa0292250 MtrunA17_Chr2g0312751 74.528 106 27 0 1 106 1 106 1.02e-56 174
Msa0292250 MtrunA17_Chr2g0312781 71.698 106 30 0 1 106 18 123 5.84e-55 170
Msa0292250 MtrunA17_Chr2g0312731 70.755 106 31 0 1 106 1 106 9.16e-55 169
Msa0292250 MtrunA17_Chr2g0312691 68.868 106 33 0 1 106 1 106 3.01e-53 166
Msa0292250 MtrunA17_Chr2g0312811 69.444 108 31 1 1 106 1 108 1.49e-52 164
Msa0292250 MtrunA17_Chr2g0312721 70.755 106 31 0 1 106 1 106 2.39e-51 160
Msa0292250 MtrunA17_Chr2g0312681 67.925 106 34 0 1 106 1 106 1.62e-50 159
Msa0292250 MtrunA17_Chr8g0357241 67.290 107 35 0 1 107 1 107 1.62e-50 159
Msa0292250 MtrunA17_Chr4g0069471 72.115 104 28 1 1 103 22 125 2.59e-50 157
Msa0292250 MtrunA17_Chr8g0357211 66.355 107 36 0 1 107 1 107 5.27e-50 157
Msa0292250 MtrunA17_Chr2g0312711 66.038 106 36 0 1 106 1 106 6.63e-49 154
Msa0292250 MtrunA17_Chr3g0107731 65.000 100 35 0 5 104 10 109 2.31e-43 140
Msa0292250 MtrunA17_Chr3g0107741 63.462 104 38 0 3 106 8 111 8.89e-43 139
Msa0292250 MtrunA17_Chr4g0029341 57.547 106 44 1 1 106 1 105 4.85e-40 132
Msa0292250 MtrunA17_Chr1g0196661 59.615 104 41 1 4 107 3 105 6.93e-39 129
Msa0292250 MtrunA17_Chr3g0132581 55.882 102 44 1 3 104 4 104 6.27e-38 127
Msa0292250 MtrunA17_Chr5g0417011 52.381 105 50 0 2 106 5 109 1.34e-37 126
Msa0292250 MtrunA17_Chr8g0379581 55.238 105 46 1 2 106 3 106 2.42e-37 125
Msa0292250 MtrunA17_Chr1g0196691 55.769 104 45 1 4 107 28 130 5.02e-36 122
Msa0292250 MtrunA17_Chr1g0196721 58.252 103 42 1 5 107 3 104 1.05e-35 121
Msa0292250 MtrunA17_Chr7g0240641 56.436 101 43 1 3 103 6 105 1.25e-35 121
Msa0292250 MtrunA17_Chr7g0240631 55.446 101 44 1 3 103 6 105 2.84e-35 120
Msa0292250 MtrunA17_Chr7g0240691 57.426 101 42 1 3 103 36 135 2.96e-35 120
Msa0292250 MtrunA17_Chr7g0240681 55.446 101 44 1 3 103 6 105 5.04e-35 119
Msa0292250 MtrunA17_Chr5g0415411 53.271 107 48 1 2 106 3 109 5.56e-34 117
Msa0292250 MtrunA17_Chr1g0196711 55.769 104 45 1 4 107 3 105 5.21e-33 114
Msa0292250 MtrunA17_Chr8g0379561 54.808 104 46 1 4 107 6 108 5.50e-33 114
Msa0292250 MtrunA17_Chr4g0008791 51.000 100 48 1 4 103 7 105 1.78e-32 113
Msa0292250 MtrunA17_Chr7g0240561 52.475 101 47 1 3 103 6 105 6.64e-32 111
Msa0292250 MtrunA17_Chr4g0008781 52.475 101 47 1 3 103 7 106 1.37e-31 110
Msa0292250 MtrunA17_Chr8g0391631 52.427 103 47 1 4 104 21 123 2.76e-31 108
Msa0292250 MtrunA17_Chr7g0240511 53.465 101 46 1 3 103 6 105 4.34e-31 109
Msa0292250 MtrunA17_Chr7g0240611 51.485 101 48 1 3 103 6 105 4.55e-31 109
Msa0292250 MtrunA17_Chr1g0212301 53.271 107 48 2 1 107 1 105 6.43e-31 108
Msa0292250 MtrunA17_Chr7g0240541 52.475 101 47 1 3 103 6 105 1.90e-30 107
Msa0292250 MtrunA17_Chr7g0240661 50.485 103 50 1 1 103 4 105 1.97e-30 107
Msa0292250 MtrunA17_Chr1g0196771 49.020 102 51 1 3 104 2 102 2.15e-30 107
Msa0292250 MtrunA17_Chr7g0240521 52.475 101 47 1 3 103 13 112 2.39e-30 107
Msa0292250 MtrunA17_Chr7g0240651 47.619 105 54 1 3 107 6 109 3.89e-30 107
Msa0292250 MtrunA17_Chr7g0240671 50.000 104 51 1 1 104 1 103 4.12e-30 107
Msa0292250 MtrunA17_Chr6g0483141 59.770 87 34 1 18 104 17 102 5.82e-30 106
Msa0292250 MtrunA17_Chr7g0240581 50.943 106 50 2 3 107 6 110 6.49e-30 106
Msa0292250 MtrunA17_Chr7g0240531 50.476 105 51 1 3 107 6 109 1.09e-29 105
Msa0292250 MtrunA17_Chr5g0433801 50.000 106 51 2 2 106 4 108 1.34e-29 105
Msa0292250 MtrunA17_Chr8g0363751 52.885 104 47 1 3 104 4 107 1.55e-29 105
Msa0292250 MtrunA17_Chr7g0240551 52.830 106 48 2 3 107 6 110 4.71e-29 104
Msa0292250 MtrunA17_Chr2g0313561 66.667 60 20 0 47 106 1 60 9.84e-28 96.7
Msa0292250 MtrunA17_Chr4g0076691 34.409 93 57 2 4 93 15 106 1.26e-11 58.5
Msa0292250 MtrunA17_Chr4g0076681 35.106 94 57 2 3 93 14 106 1.66e-11 58.5
Msa0292250 MtrunA17_Chr5g0415421 42.466 73 40 1 28 98 11 83 8.55e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0292250 AT1G78340.1 70.755 106 31 0 1 106 1 106 2.39e-54 169
Msa0292250 AT1G78320.1 68.269 104 33 0 1 104 1 104 5.17e-50 158
Msa0292250 AT1G78380.1 66.981 106 35 0 1 106 1 106 1.28e-49 157
Msa0292250 AT1G17180.1 66.038 106 36 0 1 106 1 106 2.17e-49 156
Msa0292250 AT1G17170.1 66.355 107 36 0 1 107 1 107 3.55e-49 155
Msa0292250 AT1G17190.1 64.423 104 37 0 3 106 4 107 1.14e-47 152
Msa0292250 AT1G78360.1 65.421 107 36 1 1 106 1 107 2.92e-46 148
Msa0292250 AT1G78370.1 59.813 107 43 0 1 107 1 107 3.52e-46 148
Msa0292250 AT2G29420.1 59.048 105 42 1 2 106 7 110 1.12e-41 137
Msa0292250 AT1G53680.1 59.048 105 43 0 3 107 6 110 1.32e-40 134
Msa0292250 AT3G43800.1 59.813 107 40 2 3 106 4 110 3.87e-38 128
Msa0292250 AT1G74590.1 52.885 104 49 0 3 106 6 109 2.26e-37 126
Msa0292250 AT3G09270.1 55.238 105 46 1 3 106 5 109 1.47e-36 124
Msa0292250 AT2G29490.1 53.465 101 46 1 3 103 6 105 1.03e-35 121
Msa0292250 AT2G29480.1 51.923 104 49 1 3 106 6 108 9.95e-35 119
Msa0292250 AT5G62480.1 50.980 102 50 0 1 102 5 106 2.24e-34 118
Msa0292250 AT2G29450.1 51.923 104 49 1 3 106 5 107 2.31e-34 118
Msa0292250 AT2G29460.1 50.476 105 51 1 3 107 6 109 5.84e-34 117
Msa0292250 AT1G59700.1 56.311 103 43 2 3 103 5 107 6.42e-34 117
Msa0292250 AT2G29440.1 52.885 104 48 1 3 106 5 107 1.24e-33 116
Msa0292250 AT1G10370.1 54.808 104 46 1 2 104 3 106 1.70e-33 116
Msa0292250 AT1G10360.1 55.340 103 45 1 2 103 3 105 4.31e-33 115
Msa0292250 AT1G27130.1 52.427 103 47 1 3 103 5 107 3.81e-32 112
Msa0292250 AT1G69930.1 53.000 100 47 0 5 104 13 112 1.08e-30 108
Msa0292250 AT2G29470.1 50.000 104 50 2 5 107 8 110 1.42e-30 108
Msa0292250 AT1G59670.1 54.369 103 45 2 3 103 5 107 1.52e-30 108
Msa0292250 AT1G27140.1 50.000 104 49 1 3 103 5 108 3.11e-29 105
Msa0292250 AT1G78320.2 50.962 104 24 1 1 104 1 77 1.22e-28 102
Msa0292250 AT1G69920.1 46.154 104 54 1 5 106 35 138 1.45e-27 101
Msa0292250 AT5G62480.2 35.294 102 40 1 1 102 5 80 1.57e-16 72.0
Msa0292250 AT5G62480.3 35.294 102 39 1 1 102 5 79 2.11e-16 71.6

Find 28 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAGCACAAGCTAGATTT+TGG 0.186476 2_3:-41187313 Msa0292250:CDS
ATTACAAGTGACTCACAAAT+AGG 0.297067 2_3:+41187398 None:intergenic
GATATTTAATCAGTTGAGTT+AGG 0.322462 2_3:-41187127 None:intergenic
GGTTTCTGGATAAGTCCTTA+TGG 0.328976 2_3:-41187560 Msa0292250:CDS
AAGAATAGCCCTTGCTGAAA+AGG 0.396907 2_3:-41187528 Msa0292250:CDS
TTGCCATTGTGGATTAGAAC+CGG 0.405758 2_3:+41187422 None:intergenic
CACAAATAGGTTTGCCATTG+TGG 0.406278 2_3:+41187411 None:intergenic
ATAGAGATGCAAGGGAGAAT+TGG 0.406692 2_3:-41187484 Msa0292250:CDS
AAAATCTAGCTTGTGCTCTC+TGG 0.411980 2_3:+41187315 None:intergenic
TTGAGTATATTGATGAGGTT+TGG 0.428745 2_3:-41187373 Msa0292250:CDS
ATTCTTGCTCTCATTCCATA+AGG 0.463260 2_3:+41187545 None:intergenic
GGCTGACTATGTTGATAAAA+CGG 0.464640 2_3:-41187291 Msa0292250:intron
AGAATAGCCCTTGCTGAAAA+GGG 0.508196 2_3:-41187527 Msa0292250:CDS
GGGTTCATTTGTAGAAGATA+AGG 0.510488 2_3:+41187461 None:intergenic
CTAGCTTGTGCTCTCTGGTA+GGG 0.530129 2_3:+41187320 None:intergenic
GTATATTGATGAGGTTTGGA+AGG 0.548215 2_3:-41187369 Msa0292250:CDS
GTTCATAGATAGAGATGCAA+GGG 0.548817 2_3:-41187492 Msa0292250:CDS
TGCCATTGTGGATTAGAACC+GGG 0.551246 2_3:+41187423 None:intergenic
TCTAGCTTGTGCTCTCTGGT+AGG 0.552026 2_3:+41187319 None:intergenic
CTACCAAGTCATGGCTGATG+AGG 0.563594 2_3:-41187594 None:intergenic
CCAAGTCATGGCTGATGAGG+TGG 0.571685 2_3:-41187591 None:intergenic
CCCGGTTCACAAGAAAATCC+CGG 0.589293 2_3:-41187441 Msa0292250:CDS
GCTCTCTGGTAGGGATCAGA+AGG 0.603013 2_3:+41187329 None:intergenic
AGTTCATAGATAGAGATGCA+AGG 0.614971 2_3:-41187493 Msa0292250:CDS
TTATCTTCTACAAATGAACC+CGG 0.663975 2_3:-41187459 Msa0292250:CDS
AATTGTTGAGTATATTGATG+AGG 0.670411 2_3:-41187378 Msa0292250:CDS
TAGGGATCAGAAGGCAACAA+CGG 0.683708 2_3:+41187338 None:intergenic
ATCCCGGTTCTAATCCACAA+TGG 0.688019 2_3:-41187425 Msa0292250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTGTTGAGTATATTGATG+AGG - chr2_3:41187334-41187353 Msa0292250:CDS 25.0%
TTATCTTCTACAAATGAACC+CGG - chr2_3:41187253-41187272 Msa0292250:intron 30.0%
ATTACAAGTGACTCACAAAT+AGG + chr2_3:41187317-41187336 None:intergenic 30.0%
TTGAGTATATTGATGAGGTT+TGG - chr2_3:41187339-41187358 Msa0292250:CDS 30.0%
ATTCTTGCTCTCATTCCATA+AGG + chr2_3:41187170-41187189 None:intergenic 35.0%
AGTTCATAGATAGAGATGCA+AGG - chr2_3:41187219-41187238 Msa0292250:intron 35.0%
GTTCATAGATAGAGATGCAA+GGG - chr2_3:41187220-41187239 Msa0292250:intron 35.0%
GGGTTCATTTGTAGAAGATA+AGG + chr2_3:41187254-41187273 None:intergenic 35.0%
GTATATTGATGAGGTTTGGA+AGG - chr2_3:41187343-41187362 Msa0292250:CDS 35.0%
! GGCTGACTATGTTGATAAAA+CGG - chr2_3:41187421-41187440 Msa0292250:CDS 35.0%
TTGAAACTCACATACACAAG+TGG - chr2_3:41187525-41187544 Msa0292250:CDS 35.0%
!!! AGGTGGTTTTGTTAGGTTTC+TGG - chr2_3:41187138-41187157 Msa0292250:intron 40.0%
! GGTTTCTGGATAAGTCCTTA+TGG - chr2_3:41187152-41187171 Msa0292250:intron 40.0%
AAGAATAGCCCTTGCTGAAA+AGG - chr2_3:41187184-41187203 Msa0292250:intron 40.0%
AGAATAGCCCTTGCTGAAAA+GGG - chr2_3:41187185-41187204 Msa0292250:intron 40.0%
ATAGAGATGCAAGGGAGAAT+TGG - chr2_3:41187228-41187247 Msa0292250:intron 40.0%
!! TTGCCATTGTGGATTAGAAC+CGG + chr2_3:41187293-41187312 None:intergenic 40.0%
CACAAATAGGTTTGCCATTG+TGG + chr2_3:41187304-41187323 None:intergenic 40.0%
! AAAATCTAGCTTGTGCTCTC+TGG + chr2_3:41187400-41187419 None:intergenic 40.0%
!! AGAGAGCACAAGCTAGATTT+TGG - chr2_3:41187399-41187418 Msa0292250:CDS 40.0%
!! GAGAGCACAAGCTAGATTTT+GGG - chr2_3:41187400-41187419 Msa0292250:CDS 40.0%
AACTCACATACACAAGTGGA+AGG - chr2_3:41187529-41187548 Msa0292250:CDS 40.0%
!!! GCTGATGAGGTGGTTTTGTT+AGG - chr2_3:41187131-41187150 Msa0292250:intron 45.0%
! CTTGACACCCTTTTCAGCAA+GGG + chr2_3:41187195-41187214 None:intergenic 45.0%
! ACTTGACACCCTTTTCAGCA+AGG + chr2_3:41187196-41187215 None:intergenic 45.0%
! ACCGGGATTTTCTTGTGAAC+CGG + chr2_3:41187275-41187294 None:intergenic 45.0%
! ATCCCGGTTCTAATCCACAA+TGG - chr2_3:41187287-41187306 Msa0292250:intron 45.0%
!! TGCCATTGTGGATTAGAACC+GGG + chr2_3:41187292-41187311 None:intergenic 45.0%
TAGGGATCAGAAGGCAACAA+CGG + chr2_3:41187377-41187396 None:intergenic 45.0%
CACATACACAAGTGGAAGGA+CGG - chr2_3:41187533-41187552 Msa0292250:CDS 45.0%
ACATACACAAGTGGAAGGAC+GGG - chr2_3:41187534-41187553 Msa0292250:CDS 45.0%
! CCGGGATTTTCTTGTGAACC+GGG + chr2_3:41187274-41187293 None:intergenic 50.0%
CCCGGTTCACAAGAAAATCC+CGG - chr2_3:41187271-41187290 Msa0292250:intron 50.0%
! CTAGCTTGTGCTCTCTGGTA+GGG + chr2_3:41187395-41187414 None:intergenic 50.0%
! TCTAGCTTGTGCTCTCTGGT+AGG + chr2_3:41187396-41187415 None:intergenic 50.0%
GCTCTCTGGTAGGGATCAGA+AGG + chr2_3:41187386-41187405 None:intergenic 55.0%
TACACAAGTGGAAGGACGGG+TGG - chr2_3:41187537-41187556 Msa0292250:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 41187128 41187606 41187128 ID=Msa0292250;Name=Msa0292250
chr2_3 mRNA 41187128 41187606 41187128 ID=Msa0292250-mRNA-1;Parent=Msa0292250;Name=Msa0292250-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0|0.5|1|1|2|0|107
chr2_3 exon 41187292 41187606 41187292 ID=Msa0292250-mRNA-1:exon:13314;Parent=Msa0292250-mRNA-1
chr2_3 exon 41187128 41187136 41187128 ID=Msa0292250-mRNA-1:exon:13313;Parent=Msa0292250-mRNA-1
chr2_3 CDS 41187292 41187606 41187292 ID=Msa0292250-mRNA-1:cds;Parent=Msa0292250-mRNA-1
chr2_3 CDS 41187128 41187136 41187128 ID=Msa0292250-mRNA-1:cds;Parent=Msa0292250-mRNA-1
Gene Sequence

>Msa0292250

ATGGCTGATGAGGTGGTTTTGTTAGGTTTCTGGATAAGTCCTTATGGAATGAGAGCAAGAATAGCCCTTGCTGAAAAGGGTGTCAAGTATGAGTTCATAGATAGAGATGCAAGGGAGAATTGGCCTTATCTTCTACAAATGAACCCGGTTCACAAGAAAATCCCGGTTCTAATCCACAATGGCAAACCTATTTGTGAGTCACTTGTAATTGTTGAGTATATTGATGAGGTTTGGAAGGATAGATCTCCGTTGTTGCCTTCTGATCCCTACCAGAGAGCACAAGCTAGATTTTGGGCTGACTATGTTGATAAAACGTTGAGTTAG

Protein sequence

>Msa0292250

MADEVVLLGFWISPYGMRARIALAEKGVKYEFIDRDARENWPYLLQMNPVHKKIPVLIHNGKPICESLVIVEYIDEVWKDRSPLLPSDPYQRAQARFWADYVDKTLS*