Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0293910 | AES66534.1 | 90.000 | 70 | 6 | 1 | 1 | 69 | 1 | 70 | 2.10e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0293910 | G7IR13 | 90.000 | 70 | 6 | 1 | 1 | 69 | 1 | 70 | 1.00e-31 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0293910 | Msa0461790 | 0.800738 | 1.251758e-48 | -8.615850e-47 |
| Msa0293910 | Msa0768370 | 0.807061 | 6.076657e-50 | -8.615850e-47 |
| Msa0293910 | Msa0808010 | 0.807775 | 4.286063e-50 | -8.615850e-47 |
| Msa0293910 | Msa0808020 | 0.807885 | 4.062901e-50 | -8.615850e-47 |
| Msa0293910 | Msa0848960 | 0.824266 | 8.859266e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0293910 | MtrunA17_Chr2g0315261 | 75.862 | 58 | 13 | 1 | 1 | 57 | 1 | 58 | 2.91e-20 | 76.3 |
| Msa0293910 | MtrunA17_Chr2g0315251 | 64.615 | 65 | 21 | 2 | 1 | 64 | 1 | 64 | 1.23e-17 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCATCATGGTTCGTCTAT+TGG | 0.252872 | 2_3:-45441094 | None:intergenic |
| TTCATCATGGTTCGTCTATT+GGG | 0.283951 | 2_3:-45441093 | None:intergenic |
| AACAAAGGAGAAGAAGGGTT+TGG | 0.417937 | 2_3:-45440764 | Msa0293910:CDS |
| ACAAAGGAGAAGAAGGGTTT+GGG | 0.418895 | 2_3:-45440763 | Msa0293910:CDS |
| CATTGGAACAAAGGAGAAGA+AGG | 0.453934 | 2_3:-45440770 | Msa0293910:CDS |
| AGAAATTAGTTACTTGAATG+AGG | 0.458559 | 2_3:+45441011 | None:intergenic |
| GAACAACACGTGGTGGATCA+AGG | 0.509664 | 2_3:-45440834 | Msa0293910:CDS |
| GTTCACCATTCCCAGTGCTA+AGG | 0.528785 | 2_3:+45440803 | None:intergenic |
| GGTGAACAAATTAATAACAT+TGG | 0.531564 | 2_3:-45440787 | Msa0293910:CDS |
| AGGAGGACAGACCTTAGCAC+TGG | 0.544600 | 2_3:-45440814 | Msa0293910:CDS |
| ACTTGAATGAGGATCTAACT+TGG | 0.563310 | 2_3:+45441022 | None:intergenic |
| ATTGGAACAAAGGAGAAGAA+GGG | 0.567228 | 2_3:-45440769 | Msa0293910:CDS |
| GGATCTAACTTGGCTAGACA+TGG | 0.574598 | 2_3:+45441032 | None:intergenic |
| ACAGACCTTAGCACTGGGAA+TGG | 0.585980 | 2_3:-45440808 | Msa0293910:CDS |
| AATTAATAACATTGGAACAA+AGG | 0.599700 | 2_3:-45440779 | Msa0293910:CDS |
| GGAACCAGAACAACACGTGG+TGG | 0.676062 | 2_3:-45440841 | Msa0293910:intron |
| GGAGGACAGACCTTAGCACT+GGG | 0.681970 | 2_3:-45440813 | Msa0293910:CDS |
| TTTGGAACCAGAACAACACG+TGG | 0.695096 | 2_3:-45440844 | Msa0293910:intron |
| CAACACGTGGTGGATCAAGG+AGG | 0.739219 | 2_3:-45440831 | Msa0293910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAATAGTTATGTTAATT+TGG | + | chr2_3:45440936-45440955 | None:intergenic | 10.0% |
| !!! | TGTATTTATTGTTTTTTGTT+TGG | - | chr2_3:45440957-45440976 | Msa0293910:intron | 15.0% |
| !! | AATTAATAACATTGGAACAA+AGG | - | chr2_3:45441040-45441059 | Msa0293910:CDS | 20.0% |
| ! | AGAAATTAGTTACTTGAATG+AGG | + | chr2_3:45440811-45440830 | None:intergenic | 25.0% |
| ! | TTGAACAAAAAGGTAAAGTA+GGG | + | chr2_3:45440858-45440877 | None:intergenic | 25.0% |
| ! | ATCTAGTTACTTGAACAAAA+AGG | + | chr2_3:45440868-45440887 | None:intergenic | 25.0% |
| ! | GGTGAACAAATTAATAACAT+TGG | - | chr2_3:45441032-45441051 | Msa0293910:CDS | 25.0% |
| CTTGAACAAAAAGGTAAAGT+AGG | + | chr2_3:45440859-45440878 | None:intergenic | 30.0% | |
| TAGATAAATGTACGTAGTTG+AGG | - | chr2_3:45440883-45440902 | Msa0293910:intron | 30.0% | |
| ACTTGAATGAGGATCTAACT+TGG | + | chr2_3:45440800-45440819 | None:intergenic | 35.0% | |
| AACAAAAAGGTAAAGTAGGG+AGG | + | chr2_3:45440855-45440874 | None:intergenic | 35.0% | |
| ATTGGAACAAAGGAGAAGAA+GGG | - | chr2_3:45441050-45441069 | Msa0293910:CDS | 35.0% | |
| CATTGGAACAAAGGAGAAGA+AGG | - | chr2_3:45441049-45441068 | Msa0293910:CDS | 40.0% | |
| AACAAAGGAGAAGAAGGGTT+TGG | - | chr2_3:45441055-45441074 | Msa0293910:CDS | 40.0% | |
| ACAAAGGAGAAGAAGGGTTT+GGG | - | chr2_3:45441056-45441075 | Msa0293910:CDS | 40.0% | |
| GGATCTAACTTGGCTAGACA+TGG | + | chr2_3:45440790-45440809 | None:intergenic | 45.0% | |
| ACGTAGTTGAGGTTTGTCCT+AGG | - | chr2_3:45440894-45440913 | Msa0293910:intron | 45.0% | |
| !!! | GCTACTTTTGCGTTTTGCCT+AGG | + | chr2_3:45440914-45440933 | None:intergenic | 45.0% |
| ! | TTTGGAACCAGAACAACACG+TGG | - | chr2_3:45440975-45440994 | Msa0293910:intron | 45.0% |
| ! | TGATCCACCACGTGTTGTTC+TGG | + | chr2_3:45440985-45441004 | None:intergenic | 50.0% |
| !! | GAACAACACGTGGTGGATCA+AGG | - | chr2_3:45440985-45441004 | Msa0293910:intron | 50.0% |
| ! | ACAGACCTTAGCACTGGGAA+TGG | - | chr2_3:45441011-45441030 | Msa0293910:intron | 50.0% |
| GTTCACCATTCCCAGTGCTA+AGG | + | chr2_3:45441019-45441038 | None:intergenic | 50.0% | |
| GGAACCAGAACAACACGTGG+TGG | - | chr2_3:45440978-45440997 | Msa0293910:intron | 55.0% | |
| !! | CAACACGTGGTGGATCAAGG+AGG | - | chr2_3:45440988-45441007 | Msa0293910:intron | 55.0% |
| AGGAGGACAGACCTTAGCAC+TGG | - | chr2_3:45441005-45441024 | Msa0293910:intron | 55.0% | |
| GGAGGACAGACCTTAGCACT+GGG | - | chr2_3:45441006-45441025 | Msa0293910:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 45440732 | 45441109 | 45440732 | ID=Msa0293910;Name=Msa0293910 |
| chr2_3 | mRNA | 45440732 | 45441109 | 45440732 | ID=Msa0293910-mRNA-1;Parent=Msa0293910;Name=Msa0293910-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|1|1|2|0|69 |
| chr2_3 | exon | 45441024 | 45441109 | 45441024 | ID=Msa0293910-mRNA-1:exon:14194;Parent=Msa0293910-mRNA-1 |
| chr2_3 | exon | 45440732 | 45440855 | 45440732 | ID=Msa0293910-mRNA-1:exon:14193;Parent=Msa0293910-mRNA-1 |
| chr2_3 | CDS | 45441024 | 45441109 | 45441024 | ID=Msa0293910-mRNA-1:cds;Parent=Msa0293910-mRNA-1 |
| chr2_3 | CDS | 45440732 | 45440855 | 45440732 | ID=Msa0293910-mRNA-1:cds;Parent=Msa0293910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
