AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0296270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0296270 MtrunA17_Chr2g0318901 93.773 273 17 0 1 273 1 273 0.0 545
Msa0296270 MtrunA17_Chr2g0318871 83.516 273 45 0 1 273 1 273 3.53e-177 489
Msa0296270 MtrunA17_Chr2g0318931 65.056 269 85 5 1 261 5 272 2.93e-126 360
Msa0296270 MtrunA17_Chr8g0355861 45.149 268 132 7 1 260 1 261 4.75e-78 237
Msa0296270 MtrunA17_Chr8g0355321 37.217 309 130 6 10 260 1 303 1.63e-73 227
Msa0296270 MtrunA17_Chr1g0168781 41.697 271 144 6 1 264 1 264 2.44e-72 223
Msa0296270 MtrunA17_Chr8g0355871 45.408 196 98 3 71 259 29 222 2.48e-60 191
Msa0296270 MtrunA17_Chr8g0355311 49.412 170 79 2 96 265 34 196 6.99e-58 183
Msa0296270 MtrunA17_Chr8g0355841 41.525 236 111 4 59 268 284 518 1.10e-57 192
Msa0296270 MtrunA17_Chr8g0355331 45.882 170 85 2 97 266 266 428 1.58e-53 179
Msa0296270 MtrunA17_Chr8g0355831 55.333 150 66 1 111 260 204 352 9.95e-52 172
Msa0296270 MtrunA17_Chr1g0147261 33.993 303 148 12 1 257 1 297 2.19e-45 154
Msa0296270 MtrunA17_Chr3g0139431 30.960 323 148 12 6 259 5 321 1.25e-43 150
Msa0296270 MtrunA17_Chr1g0147311 29.052 327 157 9 5 258 30 354 2.99e-40 142
Msa0296270 MtrunA17_Chr1g0147331 29.538 325 153 11 5 258 4 323 5.21e-39 139
Msa0296270 MtrunA17_Chr1g0147241 30.990 313 157 12 1 257 1 310 2.51e-38 136
Msa0296270 MtrunA17_Chr1g0146631 29.231 325 154 12 5 258 4 323 4.94e-38 136
Msa0296270 MtrunA17_Chr1g0147301 33.047 233 122 10 57 258 54 283 6.12e-32 119
Msa0296270 MtrunA17_Chr1g0147291 32.895 228 125 9 57 258 40 265 6.62e-32 118
Msa0296270 MtrunA17_Chr1g0147321 33.333 222 120 9 62 257 16 235 2.90e-31 116
Msa0296270 MtrunA17_Chr1g0147271 34.579 214 111 7 73 258 171 383 7.69e-31 118
Msa0296270 MtrunA17_Chr1g0147251 31.532 222 119 6 63 257 25 240 2.48e-30 114
Msa0296270 MtrunA17_Chr3g0144421 34.752 141 83 5 128 259 162 302 4.25e-19 85.1
Msa0296270 MtrunA17_Chr4g0034821 29.646 226 117 11 72 261 74 293 5.01e-18 82.0
Msa0296270 MtrunA17_Chr3g0138231 31.250 144 89 4 128 261 125 268 1.99e-15 74.3
Msa0296270 MtrunA17_Chr4g0034731 32.867 143 84 5 129 259 290 432 1.59e-14 73.2
Msa0296270 MtrunA17_Chr3g0144451 32.639 144 87 5 128 261 134 277 1.61e-14 72.0
Msa0296270 MtrunA17_Chr4g0034721 34.266 143 82 6 129 259 434 576 3.03e-14 72.4
Msa0296270 MtrunA17_Chr4g0076231 26.897 145 95 5 123 257 13 156 7.62e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0296270 AT5G25610.1 33.036 224 121 8 63 257 166 389 8.74e-30 115
Msa0296270 AT1G49320.1 35.135 148 81 5 126 258 131 278 1.11e-16 78.2

Find 65 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCATTTCCTTGGAAATA+AGG 0.224770 2_3:+49380204 Msa0296270:CDS
ACAAAGATTGTGCCTCTTTA+GGG 0.230864 2_3:-49380416 None:intergenic
AACAAAGATTGTGCCTCTTT+AGG 0.250870 2_3:-49380417 None:intergenic
ATGTTATAATATAATGAAAA+TGG 0.261104 2_3:+49380457 Msa0296270:three_prime_UTR
GTCTTATCAATGATAATTTC+AGG 0.264308 2_3:+49380545 Msa0296270:three_prime_UTR
AAGCTCATGTTGATAGAAAA+AGG 0.279573 2_3:-49379681 None:intergenic
CGTCCCTTTGCACAACCTTA+TGG 0.298036 2_3:+49379740 Msa0296270:CDS
CACTGCCTGAAGAAGAATAT+TGG 0.300262 2_3:+49377133 Msa0296270:CDS
TTCCTTGTTGATTGCAAGAA+TGG 0.319276 2_3:+49376771 Msa0296270:exon
TGGTGCCAATGTTGCTGTTT+AGG 0.323097 2_3:+49380267 Msa0296270:CDS
AAATCCTTGGGAACATTAAT+CGG 0.334249 2_3:+49379872 Msa0296270:CDS
GCTTCCCAATATTCTTCTTC+AGG 0.355894 2_3:-49377138 None:intergenic
AAGAATATTGGGAAGCTGTT+TGG 0.356329 2_3:+49377145 Msa0296270:CDS
GCATTGTCCAAACCTAAATT+AGG 0.363667 2_3:-49380241 None:intergenic
GTTTGTCATGCTGATACTTC+AGG 0.376770 2_3:+49380124 Msa0296270:CDS
GGATTTGCAATTTCTAAGCT+TGG 0.386794 2_3:+49379893 Msa0296270:CDS
GATGTGCAGCTTGCACTTGC+AGG 0.411503 2_3:+49377096 Msa0296270:intron
TTGTTCATGTTTATCTATGA+AGG 0.411973 2_3:-49379942 None:intergenic
ACTGCCTGAAGAAGAATATT+GGG 0.412787 2_3:+49377134 Msa0296270:CDS
TGCACGTTCATTGATTTGCC+TGG 0.415975 2_3:-49379707 None:intergenic
TATCAACATGAGCTTTATCC+AGG 0.420421 2_3:+49379689 Msa0296270:CDS
ATAGAGTCAGTGCAAAATCT+TGG 0.422753 2_3:+49379971 Msa0296270:CDS
ATGGGTACCTAATTTAGGTT+TGG 0.424424 2_3:+49380234 Msa0296270:CDS
TCCTTGTTGATTGCAAGAAT+GGG 0.433481 2_3:+49376772 Msa0296270:exon
AGAAAGCAGTTATGTGTCAT+AGG 0.437124 2_3:+49379996 Msa0296270:CDS
AAACCTGTATGTCATTTCCT+TGG 0.455480 2_3:+49380196 Msa0296270:CDS
TAGAAGTTCTCGAAGAGAAG+TGG 0.456535 2_3:-49377184 None:intergenic
CTAGCTAATTAAGCTAGAGT+TGG 0.466469 2_3:+49380373 Msa0296270:three_prime_UTR
GTGTGTCATCAATTTCCATT+GGG 0.467040 2_3:-49379646 None:intergenic
AACCTTATGGTGTTTATACA+TGG 0.471062 2_3:+49379753 Msa0296270:CDS
AAATAACAACCTTGAGGTCC+AGG 0.471918 2_3:-49377210 None:intergenic
CTTCTTCAGGCAGTGATGCA+TGG 0.473820 2_3:-49377125 None:intergenic
GAAGTGGGAATGGGAGTGTT+GGG 0.474967 2_3:-49377168 None:intergenic
GTTCCATCATTTGCCACCAA+TGG 0.478916 2_3:-49380082 None:intergenic
ACACCATAAGGTTGTGCAAA+GGG 0.481077 2_3:-49379743 None:intergenic
TTTCCAAGGAAATGACATAC+AGG 0.483607 2_3:-49380199 None:intergenic
TTAGGTTTGGACAATGCCTA+TGG 0.494021 2_3:+49380247 Msa0296270:CDS
GAACTGAAATGAAAATGATA+AGG 0.494524 2_3:-49376804 None:intergenic
TTCTCGAAGAGAAGTGGGAA+TGG 0.495855 2_3:-49377178 None:intergenic
CTTCGAGAACTTCTAAAGCC+TGG 0.497324 2_3:+49377192 Msa0296270:CDS
TGTGTGTCATCAATTTCCAT+TGG 0.497405 2_3:-49379647 None:intergenic
GGAGTACTCTCCATCATGGT+TGG 0.497653 2_3:+49380288 Msa0296270:exon
ATAAAATAGCCTTATTTCCA+AGG 0.498608 2_3:-49380213 None:intergenic
ATCCATGTATAAACACCATA+AGG 0.501910 2_3:-49379755 None:intergenic
AGAAGTGGGAATGGGAGTGT+TGG 0.505103 2_3:-49377169 None:intergenic
GTTCCATTGGTGGCAAATGA+TGG 0.508291 2_3:+49380079 Msa0296270:CDS
CAGATTATGAAAGCTGATCC+TGG 0.522799 2_3:+49380169 Msa0296270:CDS
TCTCGAAGAGAAGTGGGAAT+GGG 0.548515 2_3:-49377177 None:intergenic
TTTAGGAGTACTCTCCATCA+TGG 0.550450 2_3:+49380284 Msa0296270:exon
CTTCTAAAGCCTGGACCTCA+AGG 0.560362 2_3:+49377201 Msa0296270:CDS
AACAGCAACATTGGCACCAT+AGG 0.567015 2_3:-49380263 None:intergenic
AGAAGTTCTCGAAGAGAAGT+GGG 0.572137 2_3:-49377183 None:intergenic
GTAAAGAAAGCAGCTGCAGA+AGG 0.576977 2_3:+49379830 Msa0296270:CDS
ATTTATGTTACACAGTCCCA+TGG 0.579361 2_3:-49380316 None:intergenic
ATCATGGTTGGACACACCAT+GGG 0.579868 2_3:+49380300 Msa0296270:three_prime_UTR
GTACTCCTAAACAGCAACAT+TGG 0.581094 2_3:-49380272 None:intergenic
AGAAGGATACACATTTAATG+AGG 0.587845 2_3:+49379802 Msa0296270:CDS
CATCATGGTTGGACACACCA+TGG 0.594647 2_3:+49380299 Msa0296270:three_prime_UTR
TGTCATCAATTTCCATTGGG+AGG 0.600962 2_3:-49379643 None:intergenic
AAATCCGATTAATGTTCCCA+AGG 0.601831 2_3:-49379876 None:intergenic
CATGGTGTGTCCAACCATGA+TGG 0.616911 2_3:-49380298 None:intergenic
TGACATACAGGTTTACTACC+AGG 0.625667 2_3:-49380187 None:intergenic
TCCCATTCTTGCAATCAACA+AGG 0.631111 2_3:-49376773 None:intergenic
AACACCATAAGGTTGTGCAA+AGG 0.645055 2_3:-49379744 None:intergenic
TGAAATTGATGACCTCCCAA+TGG 0.651240 2_3:+49379631 Msa0296270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAATTTTATTTTTTTAA+AGG + chr2_3:49378538-49378557 Msa0296270:intron 0.0%
!!! ATATTTTTTTTAAAAAAAAT+AGG - chr2_3:49378581-49378600 None:intergenic 0.0%
!!! ATTTTTAAAAATATTTTTTT+TGG + chr2_3:49378918-49378937 Msa0296270:intron 0.0%
!! AAGAAAGAAAATTATATTTA+TGG + chr2_3:49377427-49377446 Msa0296270:intron 10.0%
!!! AAATGTAATATTTTATAGTA+TGG - chr2_3:49377613-49377632 None:intergenic 10.0%
!!! TTTTTTTTTTTTTAGTCTTT+CGG - chr2_3:49378130-49378149 None:intergenic 10.0%
!!! TTTTTGAAAATAAAAAATTC+AGG + chr2_3:49378659-49378678 Msa0296270:intron 10.0%
!!! TTTGAAAATTTTGTAAAAAA+TGG + chr2_3:49378763-49378782 Msa0296270:intron 10.0%
!!! TTAATTTATTTTAAAGATTC+TGG - chr2_3:49379022-49379041 None:intergenic 10.0%
!! AAAAAATCAGAAAAAATATT+TGG - chr2_3:49379057-49379076 None:intergenic 10.0%
!!! ACGATTTTTTTAAAAAATTT+TGG - chr2_3:49379103-49379122 None:intergenic 10.0%
!!! TATTTGATTCTAAAATTTTT+TGG + chr2_3:49379158-49379177 Msa0296270:intron 10.0%
!! TTAAAAAATAAAATATGATG+TGG + chr2_3:49379250-49379269 Msa0296270:intron 10.0%
!! ATGTTATAATATAATGAAAA+TGG + chr2_3:49380457-49380476 Msa0296270:three_prime_UTR 10.0%
!! TTAAACAACAACTTAAAATA+TGG + chr2_3:49376928-49376947 Msa0296270:intron 15.0%
!! ATCTCAATACTAATTTATAT+AGG - chr2_3:49377309-49377328 None:intergenic 15.0%
!! AATTTATGTAAGATCTAAAA+AGG + chr2_3:49377336-49377355 Msa0296270:intron 15.0%
!!! ATCTAAAAAGGTTAATTTTA+TGG + chr2_3:49377348-49377367 Msa0296270:intron 15.0%
!!! TCTAAAAAGGTTAATTTTAT+GGG + chr2_3:49377349-49377368 Msa0296270:intron 15.0%
!! ATAAAATATTACATTTGAAG+AGG + chr2_3:49377617-49377636 Msa0296270:intron 15.0%
!!! ATAGAGATATTATGTTTTTA+TGG + chr2_3:49377701-49377720 Msa0296270:intron 15.0%
!!! ATTTTTTCTTATTTTAATCG+TGG - chr2_3:49377917-49377936 None:intergenic 15.0%
!!! CACTTTTATCAATAAATTAT+AGG + chr2_3:49378025-49378044 Msa0296270:intron 15.0%
!!! TTTTGTAGTTTTTTTTCTTA+GGG - chr2_3:49378070-49378089 None:intergenic 15.0%
!!! ATTTTGTAGTTTTTTTTCTT+AGG - chr2_3:49378071-49378090 None:intergenic 15.0%
!!! TTTTTTTTTTTTAGTCTTTC+GGG - chr2_3:49378129-49378148 None:intergenic 15.0%
!! AATTAAGCCTAAATTATAAA+GGG + chr2_3:49378314-49378333 Msa0296270:intron 15.0%
!!! TTTTATTTTTTTAAAGGTAC+AGG + chr2_3:49378544-49378563 Msa0296270:intron 15.0%
!!! TATTTCTACAAAAAAATTTC+TGG - chr2_3:49378899-49378918 None:intergenic 15.0%
!!! TGAATATTTTTCTGATTTTT+TGG + chr2_3:49379190-49379209 Msa0296270:intron 15.0%
!!! AATCAAACCAAAAATTTTAT+AGG + chr2_3:49379392-49379411 Msa0296270:intron 15.0%
!!! ATCAAACCAAAAATTTTATA+GGG + chr2_3:49379393-49379412 Msa0296270:intron 15.0%
!! CACGTTATTTATTTATAATT+CGG - chr2_3:49379467-49379486 None:intergenic 15.0%
!!! GCAAATAAAGTATTATTTGT+GGG + chr2_3:49376950-49376969 Msa0296270:intron 20.0%
!!! CAAATAAAGTATTATTTGTG+GGG + chr2_3:49376951-49376970 Msa0296270:intron 20.0%
!!! AAATAAAGTATTATTTGTGG+GGG + chr2_3:49376952-49376971 Msa0296270:intron 20.0%
!! AAAATAAAATAACAACCTTG+AGG - chr2_3:49377219-49377238 None:intergenic 20.0%
!! TTATATTTATGGTACATAAC+AGG + chr2_3:49377438-49377457 Msa0296270:intron 20.0%
!! GTTTCACTCTATAAATAAAA+TGG - chr2_3:49377542-49377561 None:intergenic 20.0%
!!! TCTATCAAGTAATTTTTTGT+TGG - chr2_3:49377686-49377705 None:intergenic 20.0%
!!! TATGGTTAATCAAATTTTGT+TGG + chr2_3:49377719-49377738 Msa0296270:intron 20.0%
!! AAGAAAAAATAGCTAAAAGT+TGG + chr2_3:49377927-49377946 Msa0296270:intron 20.0%
!!! TTTGTAGTTTTTTTTCTTAG+GGG - chr2_3:49378069-49378088 None:intergenic 20.0%
!!! TTTTTTTTTTTAGTCTTTCG+GGG - chr2_3:49378128-49378147 None:intergenic 20.0%
!! AAAAAAAAAAAAAACGCATG+TGG + chr2_3:49378137-49378156 Msa0296270:intron 20.0%
!! CAATTAAGCCTAAATTATAA+AGG + chr2_3:49378313-49378332 Msa0296270:intron 20.0%
!! CTATTAACCCTTTATAATTT+AGG - chr2_3:49378324-49378343 None:intergenic 20.0%
!!! TCCAAACTGAAAATTTTATA+GGG - chr2_3:49378388-49378407 None:intergenic 20.0%
!!! ATCCAAACTGAAAATTTTAT+AGG - chr2_3:49378389-49378408 None:intergenic 20.0%
!!! AAATAAAAATTTTACGAGGA+CGG - chr2_3:49378413-49378432 None:intergenic 20.0%
!!! CTCGTAAAATTTTTATTTTC+CGG + chr2_3:49378415-49378434 Msa0296270:intron 20.0%
!!! TCAAACCAAAAATTTTATAG+GGG + chr2_3:49379394-49379413 Msa0296270:intron 20.0%
!! ATTAAAGACATTGAAAAAGA+AGG + chr2_3:49379785-49379804 Msa0296270:CDS 20.0%
!!! TTTTATTGTCATGAAATTCA+TGG + chr2_3:49380040-49380059 Msa0296270:CDS 20.0%
!! AAAAGAAAAATTCTACAATG+AGG - chr2_3:49380489-49380508 None:intergenic 20.0%
!!! CATTGTAGAATTTTTCTTTT+TGG + chr2_3:49380489-49380508 Msa0296270:three_prime_UTR 20.0%
! GAACTGAAATGAAAATGATA+AGG - chr2_3:49376807-49376826 None:intergenic 25.0%
! TAAGTTTATAGTACATCAGT+TGG - chr2_3:49376911-49376930 None:intergenic 25.0%
!! GGCAAATAAAGTATTATTTG+TGG + chr2_3:49376949-49376968 Msa0296270:intron 25.0%
! TATAAATAAAATGGTCTCTG+AGG - chr2_3:49377533-49377552 None:intergenic 25.0%
! TATAGAGTGAAACAATTCTA+AGG + chr2_3:49377549-49377568 Msa0296270:intron 25.0%
! ATAGAGTGAAACAATTCTAA+GGG + chr2_3:49377550-49377569 Msa0296270:intron 25.0%
!!! GATCCTTTTAATTTAGATAG+AGG - chr2_3:49377576-49377595 None:intergenic 25.0%
! TTGTTAAGATGAAAGATGTT+TGG - chr2_3:49377645-49377664 None:intergenic 25.0%
!!! CTCTTTCTTTTTAGCAATAT+AGG + chr2_3:49377889-49377908 Msa0296270:intron 25.0%
! TCAAATGAACGATCTATTTA+AGG + chr2_3:49377960-49377979 Msa0296270:intron 25.0%
!!! TCTTTGTTTTCCAAACATTA+AGG + chr2_3:49377985-49378004 Msa0296270:intron 25.0%
!!! TTGTAGTTTTTTTTCTTAGG+GGG - chr2_3:49378068-49378087 None:intergenic 25.0%
!!! GGTTTTGTAGTTTTTTCTTA+GGG + chr2_3:49378248-49378267 Msa0296270:intron 25.0%
!!! GTTTTGTAGTTTTTTCTTAG+GGG + chr2_3:49378249-49378268 Msa0296270:intron 25.0%
!!! TTTTGTAGTTTTTTCTTAGG+GGG + chr2_3:49378250-49378269 Msa0296270:intron 25.0%
!! GTTTTTCACACTTGTAATAT+AGG + chr2_3:49378345-49378364 Msa0296270:intron 25.0%
!! CCAAACTGAAAATTTTATAG+GGG - chr2_3:49378387-49378406 None:intergenic 25.0%
!! CGGAAAATAAAAATTTTACG+AGG - chr2_3:49378417-49378436 None:intergenic 25.0%
!!! TATTTTTTTAAAGGTACAGG+TGG + chr2_3:49378547-49378566 Msa0296270:intron 25.0%
!!! CCTTGTAAAACTTTTTTTTC+CGG - chr2_3:49379367-49379386 None:intergenic 25.0%
! CAGAAATGATCTATATTACA+GGG + chr2_3:49379424-49379443 Msa0296270:intron 25.0%
! AGAAATGATCTATATTACAG+GGG + chr2_3:49379425-49379444 Msa0296270:intron 25.0%
!!! TTTTTAATTTACTACCATCG+TGG + chr2_3:49379515-49379534 Msa0296270:intron 25.0%
! ACTCTTATATCTGCTAATTT+CGG - chr2_3:49379561-49379580 None:intergenic 25.0%
! ATAAGAGTCAGAATGAATTA+TGG + chr2_3:49379573-49379592 Msa0296270:intron 25.0%
! AAACCTATTTATTATGACAC+AGG + chr2_3:49379605-49379624 Msa0296270:intron 25.0%
!! AAAATTTTGTGCAAAATCCT+TGG + chr2_3:49379859-49379878 Msa0296270:CDS 25.0%
!! AAATTTTGTGCAAAATCCTT+GGG + chr2_3:49379860-49379879 Msa0296270:CDS 25.0%
! TTGTTCATGTTTATCTATGA+AGG - chr2_3:49379945-49379964 None:intergenic 25.0%
!!! TCATAGGTTGAATTTTCAAA+AGG + chr2_3:49380012-49380031 Msa0296270:CDS 25.0%
!!! ATAAAATAGCCTTATTTCCA+AGG - chr2_3:49380216-49380235 None:intergenic 25.0%
!!! TTGGAAATAAGGCTATTTTA+TGG + chr2_3:49380215-49380234 Msa0296270:CDS 25.0%
!!! TGGAAATAAGGCTATTTTAT+GGG + chr2_3:49380216-49380235 Msa0296270:CDS 25.0%
!! ATTTTATGGGTACCTAATTT+AGG + chr2_3:49380229-49380248 Msa0296270:CDS 25.0%
!! TTTGGTGAGTAAATGTTTTT+AGG + chr2_3:49380507-49380526 Msa0296270:three_prime_UTR 25.0%
!!! TTGGTGAGTAAATGTTTTTA+GGG + chr2_3:49380508-49380527 Msa0296270:three_prime_UTR 25.0%
!!! TTTTTAGGGGTAATTATGTT+TGG + chr2_3:49380522-49380541 Msa0296270:three_prime_UTR 25.0%
!!! TTTTAGGGGTAATTATGTTT+GGG + chr2_3:49380523-49380542 Msa0296270:three_prime_UTR 25.0%
! GTCTTATCAATGATAATTTC+AGG + chr2_3:49380545-49380564 Msa0296270:three_prime_UTR 25.0%
!! AGAATTCATAAGTGTTGAGA+GGG - chr2_3:49376875-49376894 None:intergenic 30.0%
!! AAGAATTCATAAGTGTTGAG+AGG - chr2_3:49376876-49376895 None:intergenic 30.0%
TCTCAACACTTATGAATTCT+TGG + chr2_3:49376875-49376894 Msa0296270:intron 30.0%
!!! ACTAATTTTTTAGTCTCTGC+AGG - chr2_3:49377285-49377304 None:intergenic 30.0%
GTTACCAATAGAATGAATGT+TGG - chr2_3:49377465-49377484 None:intergenic 30.0%
TCTTATCCTTTATTGCAGTA+TGG + chr2_3:49377499-49377518 Msa0296270:intron 30.0%
TAGTATGGTGTTCTAATTGT+AGG - chr2_3:49377598-49377617 None:intergenic 30.0%
AATAGATCGTTCATTTGAGA+TGG - chr2_3:49377958-49377977 None:intergenic 30.0%
CGACTAATAACCTTAATGTT+TGG - chr2_3:49377998-49378017 None:intergenic 30.0%
!! CGGTTTTGTAGTTTTTTCTT+AGG + chr2_3:49378247-49378266 Msa0296270:intron 30.0%
! CTTGTAATATAGGCTATTTC+CGG + chr2_3:49378355-49378374 Msa0296270:intron 30.0%
! CCCCTATAAAATTTTCAGTT+TGG + chr2_3:49378384-49378403 Msa0296270:intron 30.0%
! CCGGAAAAAAAAGTTTTACA+AGG + chr2_3:49379364-49379383 Msa0296270:intron 30.0%
! TTTCGCCCCTATAAAATTTT+TGG - chr2_3:49378379-49378398 None:intergenic 30.0%
CCTGTAATATAGATCATTTC+TGG - chr2_3:49379426-49379445 None:intergenic 30.0%
CCAGAAATGATCTATATTAC+AGG + chr2_3:49379423-49379442 Msa0296270:intron 30.0%
ACACCTGTGTCATAATAAAT+AGG - chr2_3:49379611-49379630 None:intergenic 30.0%
!! GTTGATAGAAAAAGGCTTTT+GGG - chr2_3:49379676-49379695 None:intergenic 30.0%
!! TGTTGATAGAAAAAGGCTTT+TGG - chr2_3:49379677-49379696 None:intergenic 30.0%
! AAGCTCATGTTGATAGAAAA+AGG - chr2_3:49379684-49379703 None:intergenic 30.0%
AACCTTATGGTGTTTATACA+TGG + chr2_3:49379753-49379772 Msa0296270:CDS 30.0%
ATCCATGTATAAACACCATA+AGG - chr2_3:49379758-49379777 None:intergenic 30.0%
AGAAGGATACACATTTAATG+AGG + chr2_3:49379802-49379821 Msa0296270:CDS 30.0%
AAATCCTTGGGAACATTAAT+CGG + chr2_3:49379872-49379891 Msa0296270:CDS 30.0%
! AAATGATGGAACAAAAACTC+AGG + chr2_3:49380093-49380112 Msa0296270:CDS 30.0%
! ATGTCATTTCCTTGGAAATA+AGG + chr2_3:49380204-49380223 Msa0296270:CDS 30.0%
! TACTTTTATGTACCCTAAAG+AGG + chr2_3:49380404-49380423 Msa0296270:three_prime_UTR 30.0%
!!! TGGTGAGTAAATGTTTTTAG+GGG + chr2_3:49380509-49380528 Msa0296270:three_prime_UTR 30.0%
! TTCCTTGTTGATTGCAAGAA+TGG + chr2_3:49376771-49376790 Msa0296270:exon 35.0%
! TCCTTGTTGATTGCAAGAAT+GGG + chr2_3:49376772-49376791 Msa0296270:exon 35.0%
! AAAGTATTATTTGTGGGGGT+TGG + chr2_3:49376956-49376975 Msa0296270:intron 35.0%
ACTGCCTGAAGAAGAATATT+GGG + chr2_3:49377134-49377153 Msa0296270:CDS 35.0%
AAGAATATTGGGAAGCTGTT+TGG + chr2_3:49377145-49377164 Msa0296270:CDS 35.0%
AGGTCCAACATTCATTCTAT+TGG + chr2_3:49377458-49377477 Msa0296270:intron 35.0%
GGGCCTCTATCTAAATTAAA+AGG + chr2_3:49377570-49377589 Msa0296270:intron 35.0%
GGGGAATCATTCATTTAACA+AGG + chr2_3:49377793-49377812 Msa0296270:intron 35.0%
!! ACCCATTTTTCTTCCTCTAA+CGG - chr2_3:49377839-49377858 None:intergenic 35.0%
! CAAAATTGCCCCTTAAGTTT+TGG + chr2_3:49378085-49378104 Msa0296270:intron 35.0%
CTACAGTTCCAAAACTTAAG+GGG - chr2_3:49378096-49378115 None:intergenic 35.0%
GCTACAGTTCCAAAACTTAA+GGG - chr2_3:49378097-49378116 None:intergenic 35.0%
TGCTACAGTTCCAAAACTTA+AGG - chr2_3:49378098-49378117 None:intergenic 35.0%
!!! TTTTGGAACTGTAGCAGTTT+TGG + chr2_3:49378102-49378121 Msa0296270:intron 35.0%
! CTTTTCCAAAGTTGCGATTT+TGG - chr2_3:49378276-49378295 None:intergenic 35.0%
! ATCGCAACTTTGGAAAAGTT+AGG + chr2_3:49378278-49378297 Msa0296270:intron 35.0%
! TCGCAACTTTGGAAAAGTTA+GGG + chr2_3:49378279-49378298 Msa0296270:intron 35.0%
! AATTTTATAGGGGCGAAAAC+CGG - chr2_3:49379404-49379423 None:intergenic 35.0%
GCAAATATGCACAGTCATAT+GGG - chr2_3:49378454-49378473 None:intergenic 35.0%
AGCAAATATGCACAGTCATA+TGG - chr2_3:49378455-49378474 None:intergenic 35.0%
ACTGTGCATATATGCTTATG+TGG + chr2_3:49378501-49378520 Msa0296270:intron 35.0%
GTGTGTCATCAATTTCCATT+GGG - chr2_3:49379649-49379668 None:intergenic 35.0%
TGTGTGTCATCAATTTCCAT+TGG - chr2_3:49379650-49379669 None:intergenic 35.0%
! TATCAACATGAGCTTTATCC+AGG + chr2_3:49379689-49379708 Msa0296270:CDS 35.0%
AAATCCGATTAATGTTCCCA+AGG - chr2_3:49379879-49379898 None:intergenic 35.0%
GGATTTGCAATTTCTAAGCT+TGG + chr2_3:49379893-49379912 Msa0296270:CDS 35.0%
ATAGAGTCAGTGCAAAATCT+TGG + chr2_3:49379971-49379990 Msa0296270:CDS 35.0%
AGAAAGCAGTTATGTGTCAT+AGG + chr2_3:49379996-49380015 Msa0296270:CDS 35.0%
! TCATGGAACAACAGCTTTTA+TGG + chr2_3:49380057-49380076 Msa0296270:CDS 35.0%
! AACAGCTTTTATGGTTCCAT+TGG + chr2_3:49380066-49380085 Msa0296270:CDS 35.0%
! AAACCTGTATGTCATTTCCT+TGG + chr2_3:49380196-49380215 Msa0296270:CDS 35.0%
TTTCCAAGGAAATGACATAC+AGG - chr2_3:49380202-49380221 None:intergenic 35.0%
! ATGGGTACCTAATTTAGGTT+TGG + chr2_3:49380234-49380253 Msa0296270:CDS 35.0%
GCATTGTCCAAACCTAAATT+AGG - chr2_3:49380244-49380263 None:intergenic 35.0%
ATTTATGTTACACAGTCCCA+TGG - chr2_3:49380319-49380338 None:intergenic 35.0%
CTAGCTAATTAAGCTAGAGT+TGG + chr2_3:49380373-49380392 Msa0296270:three_prime_UTR 35.0%
ACAAAGATTGTGCCTCTTTA+GGG - chr2_3:49380419-49380438 None:intergenic 35.0%
AACAAAGATTGTGCCTCTTT+AGG - chr2_3:49380420-49380439 None:intergenic 35.0%
TCCCATTCTTGCAATCAACA+AGG - chr2_3:49376776-49376795 None:intergenic 40.0%
CACTGCCTGAAGAAGAATAT+TGG + chr2_3:49377133-49377152 Msa0296270:CDS 40.0%
GCTTCCCAATATTCTTCTTC+AGG - chr2_3:49377141-49377160 None:intergenic 40.0%
AGAAGTTCTCGAAGAGAAGT+GGG - chr2_3:49377186-49377205 None:intergenic 40.0%
TAGAAGTTCTCGAAGAGAAG+TGG - chr2_3:49377187-49377206 None:intergenic 40.0%
AAATAACAACCTTGAGGTCC+AGG - chr2_3:49377213-49377232 None:intergenic 40.0%
! ACTGTTAGAGCATCTTGAGT+GGG + chr2_3:49377773-49377792 Msa0296270:intron 40.0%
!! CCATTTTTCTTCCTCTAACG+GGG - chr2_3:49377837-49377856 None:intergenic 40.0%
!! CCCATTTTTCTTCCTCTAAC+GGG - chr2_3:49377838-49377857 None:intergenic 40.0%
CCCGTTAGAGGAAGAAAAAT+GGG + chr2_3:49377835-49377854 Msa0296270:intron 40.0%
! GATCGTTCATTTGAGATGGT+GGG - chr2_3:49377954-49377973 None:intergenic 40.0%
! AGATCGTTCATTTGAGATGG+TGG - chr2_3:49377955-49377974 None:intergenic 40.0%
! CGCAACTTTGGAAAAGTTAG+GGG + chr2_3:49378280-49378299 Msa0296270:intron 40.0%
! GCAACTTTGGAAAAGTTAGG+GGG + chr2_3:49378281-49378300 Msa0296270:intron 40.0%
ACTCATCAAAAACGCCTATG+TGG - chr2_3:49379323-49379342 None:intergenic 40.0%
! CATAGGCGTTTTTGATGAGT+TGG + chr2_3:49379323-49379342 Msa0296270:intron 40.0%
!! GTTTTTGATGAGTTGGATGG+AGG + chr2_3:49379330-49379349 Msa0296270:intron 40.0%
! TGAAATTGATGACCTCCCAA+TGG + chr2_3:49379631-49379650 Msa0296270:CDS 40.0%
TGTCATCAATTTCCATTGGG+AGG - chr2_3:49379646-49379665 None:intergenic 40.0%
ACACCATAAGGTTGTGCAAA+GGG - chr2_3:49379746-49379765 None:intergenic 40.0%
AACACCATAAGGTTGTGCAA+AGG - chr2_3:49379747-49379766 None:intergenic 40.0%
! AGCTTTTATGGTTCCATTGG+TGG + chr2_3:49380069-49380088 Msa0296270:CDS 40.0%
GTTTGTCATGCTGATACTTC+AGG + chr2_3:49380124-49380143 Msa0296270:CDS 40.0%
CAGATTATGAAAGCTGATCC+TGG + chr2_3:49380169-49380188 Msa0296270:CDS 40.0%
TGACATACAGGTTTACTACC+AGG - chr2_3:49380190-49380209 None:intergenic 40.0%
TTAGGTTTGGACAATGCCTA+TGG + chr2_3:49380247-49380266 Msa0296270:CDS 40.0%
GTACTCCTAAACAGCAACAT+TGG - chr2_3:49380275-49380294 None:intergenic 40.0%
!! TTTAGGAGTACTCTCCATCA+TGG + chr2_3:49380284-49380303 Msa0296270:exon 40.0%
!! CATAAGTGTTGAGAGGGTCA+TGG - chr2_3:49376869-49376888 None:intergenic 45.0%
TCTCGAAGAGAAGTGGGAAT+GGG - chr2_3:49377180-49377199 None:intergenic 45.0%
TTCTCGAAGAGAAGTGGGAA+TGG - chr2_3:49377181-49377200 None:intergenic 45.0%
!! CTTCGAGAACTTCTAAAGCC+TGG + chr2_3:49377192-49377211 Msa0296270:CDS 45.0%
GCAGCACCATACTGCAATAA+AGG - chr2_3:49377508-49377527 None:intergenic 45.0%
! GACTGTTAGAGCATCTTGAG+TGG + chr2_3:49377772-49377791 Msa0296270:intron 45.0%
! CTGTTAGAGCATCTTGAGTG+GGG + chr2_3:49377774-49377793 Msa0296270:intron 45.0%
CCCCGTTAGAGGAAGAAAAA+TGG + chr2_3:49377834-49377853 Msa0296270:intron 45.0%
!! AGCGTTGACCGAGTCAAAAT+TGG + chr2_3:49378183-49378202 Msa0296270:intron 45.0%
! TTTTGCACCTGTGGCAGTTT+TGG - chr2_3:49378218-49378237 None:intergenic 45.0%
TGCCACAGGTGCAAAACATA+AGG + chr2_3:49378222-49378241 Msa0296270:intron 45.0%
GCCACAGGTGCAAAACATAA+GGG + chr2_3:49378223-49378242 Msa0296270:intron 45.0%
CCACAGGTGCAAAACATAAG+GGG + chr2_3:49378224-49378243 Msa0296270:intron 45.0%
ATGCACAGTCATATGGGAGA+GGG - chr2_3:49378448-49378467 None:intergenic 45.0%
TATGCACAGTCATATGGGAG+AGG - chr2_3:49378449-49378468 None:intergenic 45.0%
CATCAAAAACGCCTATGTGG+CGG - chr2_3:49379320-49379339 None:intergenic 45.0%
!! GGCGTTTTTGATGAGTTGGA+TGG + chr2_3:49379327-49379346 Msa0296270:intron 45.0%
! TTTGATGAGTTGGATGGAGG+AGG + chr2_3:49379333-49379352 Msa0296270:intron 45.0%
! TTGATGAGTTGGATGGAGGA+GGG + chr2_3:49379334-49379353 Msa0296270:intron 45.0%
! GTTAGTTAGAGCATCCACGA+TGG - chr2_3:49379532-49379551 None:intergenic 45.0%
!! TGCACGTTCATTGATTTGCC+TGG - chr2_3:49379710-49379729 None:intergenic 45.0%
GTAAAGAAAGCAGCTGCAGA+AGG + chr2_3:49379830-49379849 Msa0296270:CDS 45.0%
! GTTCCATTGGTGGCAAATGA+TGG + chr2_3:49380079-49380098 Msa0296270:CDS 45.0%
GTTCCATCATTTGCCACCAA+TGG - chr2_3:49380085-49380104 None:intergenic 45.0%
AACAGCAACATTGGCACCAT+AGG - chr2_3:49380266-49380285 None:intergenic 45.0%
!! TGGTGCCAATGTTGCTGTTT+AGG + chr2_3:49380267-49380286 Msa0296270:CDS 45.0%
ATCATGGTTGGACACACCAT+GGG + chr2_3:49380300-49380319 Msa0296270:three_prime_UTR 45.0%
!!! TATTTTATTTTTAAAAATCT+GGG - chr2_3:49378872-49378891 None:intergenic 5.0%
!!! ATATTTTATTTTTAAAAATC+TGG - chr2_3:49378873-49378892 None:intergenic 5.0%
GAGAGGGTCATGGAGATGAA+AGG - chr2_3:49376859-49376878 None:intergenic 50.0%
CTTCTTCAGGCAGTGATGCA+TGG - chr2_3:49377128-49377147 None:intergenic 50.0%
!! GAAGTGGGAATGGGAGTGTT+GGG - chr2_3:49377171-49377190 None:intergenic 50.0%
! AGAAGTGGGAATGGGAGTGT+TGG - chr2_3:49377172-49377191 None:intergenic 50.0%
!! CTTCTAAAGCCTGGACCTCA+AGG + chr2_3:49377201-49377220 Msa0296270:CDS 50.0%
ACGCATGTGGTACCTCACAT+AGG + chr2_3:49378150-49378169 Msa0296270:intron 50.0%
CGCATGTGGTACCTCACATA+GGG + chr2_3:49378151-49378170 Msa0296270:intron 50.0%
TGACCGAGTCAAAATTGGCC+TGG + chr2_3:49378188-49378207 Msa0296270:intron 50.0%
! GACCGAGTCAAAATTGGCCT+GGG + chr2_3:49378189-49378208 Msa0296270:intron 50.0%
! ACCGAGTCAAAATTGGCCTG+GGG + chr2_3:49378190-49378209 Msa0296270:intron 50.0%
! CCCCTTATGTTTTGCACCTG+TGG - chr2_3:49378227-49378246 None:intergenic 50.0%
CAGGTGCAAAACATAAGGGG+CGG + chr2_3:49378227-49378246 Msa0296270:intron 50.0%
!!! TATGGGAGAGGGGTGTTTTC+CGG - chr2_3:49378437-49378456 None:intergenic 50.0%
TGCACAGTCATATGGGAGAG+GGG - chr2_3:49378447-49378466 None:intergenic 50.0%
TGCTGACGTAGTACACTGTG+TGG + chr2_3:49378471-49378490 Msa0296270:intron 50.0%
! TGATGAGTTGGATGGAGGAG+GGG + chr2_3:49379335-49379354 Msa0296270:intron 50.0%
CGTCCCTTTGCACAACCTTA+TGG + chr2_3:49379740-49379759 Msa0296270:CDS 50.0%
! GGAGTACTCTCCATCATGGT+TGG + chr2_3:49380288-49380307 Msa0296270:exon 50.0%
CATGGTGTGTCCAACCATGA+TGG - chr2_3:49380301-49380320 None:intergenic 50.0%
CATCATGGTTGGACACACCA+TGG + chr2_3:49380299-49380318 Msa0296270:three_prime_UTR 50.0%
GATGTGCAGCTTGCACTTGC+AGG + chr2_3:49377096-49377115 Msa0296270:intron 55.0%
CCTCTAACGGGGCTCTTAAC+AGG - chr2_3:49377826-49377845 None:intergenic 55.0%
CCTGTTAAGAGCCCCGTTAG+AGG + chr2_3:49377823-49377842 Msa0296270:intron 55.0%
!!! CCCCCAGGCCAATTTTGACT+CGG - chr2_3:49378194-49378213 None:intergenic 55.0%
! CCGAGTCAAAATTGGCCTGG+GGG + chr2_3:49378191-49378210 Msa0296270:intron 55.0%
! CGAGTCAAAATTGGCCTGGG+GGG + chr2_3:49378192-49378211 Msa0296270:intron 55.0%
GGGGGCCAAAATCGCAACTT+TGG + chr2_3:49378268-49378287 Msa0296270:intron 55.0%
AAGCCAAGTCACCGCCACAT+AGG + chr2_3:49379306-49379325 Msa0296270:intron 55.0%
!!! GATGGAGGAGGGGTGTTTTC+CGG + chr2_3:49379345-49379364 Msa0296270:intron 55.0%
CTGACGTGGCACCCTATGTG+AGG - chr2_3:49378165-49378184 None:intergenic 60.0%
!! TGACTCGGTCAACGCTGACG+TGG - chr2_3:49378179-49378198 None:intergenic 60.0%
ACGCCTATGTGGCGGTGACT+TGG - chr2_3:49379312-49379331 None:intergenic 60.0%
!! TGTGGCAGTTTTGGCCCCCC+AGG - chr2_3:49378209-49378228 None:intergenic 65.0%
TGGGGGGCCAAAACTGCCAC+AGG + chr2_3:49378208-49378227 Msa0296270:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 49376744 49380586 49376744 ID=Msa0296270;Name=Msa0296270
chr2_3 mRNA 49376744 49380586 49376744 ID=Msa0296270-mRNA-1;Parent=Msa0296270;Name=Msa0296270-mRNA-1;_AED=0.16;_eAED=0.16;_QI=46|1|1|1|1|1|3|298|273
chr2_3 exon 49376744 49376831 49376744 ID=Msa0296270-mRNA-1:exon:15534;Parent=Msa0296270-mRNA-1
chr2_3 exon 49377105 49377222 49377105 ID=Msa0296270-mRNA-1:exon:15535;Parent=Msa0296270-mRNA-1
chr2_3 exon 49379627 49380586 49379627 ID=Msa0296270-mRNA-1:exon:15536;Parent=Msa0296270-mRNA-1
chr2_3 five_prime_UTR 49376744 49376789 49376744 ID=Msa0296270-mRNA-1:five_prime_utr;Parent=Msa0296270-mRNA-1
chr2_3 CDS 49376790 49376831 49376790 ID=Msa0296270-mRNA-1:cds;Parent=Msa0296270-mRNA-1
chr2_3 CDS 49377105 49377222 49377105 ID=Msa0296270-mRNA-1:cds;Parent=Msa0296270-mRNA-1
chr2_3 CDS 49379627 49380288 49379627 ID=Msa0296270-mRNA-1:cds;Parent=Msa0296270-mRNA-1
chr2_3 three_prime_UTR 49380289 49380586 49380289 ID=Msa0296270-mRNA-1:three_prime_utr;Parent=Msa0296270-mRNA-1
Gene Sequence

>Msa0296270

ATGGGATTTCATCACCTTATCATTTTCATTTCAGTTCTCTCTCTTGCACTTGCAGGAGAAAGCCATGCATCACTGCCTGAAGAAGAATATTGGGAAGCTGTTTGGCCCAACACTCCCATTCCCACTTCTCTTCGAGAACTTCTAAAGCCTGGACCTCAAGGTGTTGAAATTGATGACCTCCCAATGGAAATTGATGACACACAATACCCAAAAGCCTTTTTCTATCAACATGAGCTTTATCCAGGCAAATCAATGAACGTGCAATTCAGCAAGCGTCCCTTTGCACAACCTTATGGTGTTTATACATGGATGCGTGAAATTAAAGACATTGAAAAAGAAGGATACACATTTAATGAGGTATGTGTAAAGAAAGCAGCTGCAGAAGGAGAGCAAAAATTTTGTGCAAAATCCTTGGGAACATTAATCGGATTTGCAATTTCTAAGCTTGGAAAAAATATTCAAGCACTTTCAAGCTCCTTCATAGATAAACATGAACAATACAAAATAGAGTCAGTGCAAAATCTTGGAGAGAAAGCAGTTATGTGTCATAGGTTGAATTTTCAAAAGGTTGTATTTTATTGTCATGAAATTCATGGAACAACAGCTTTTATGGTTCCATTGGTGGCAAATGATGGAACAAAAACTCAGGCACTCGCTGTTTGTCATGCTGATACTTCAGGCATGAATCATGAAATGCTGCAACAGATTATGAAAGCTGATCCTGGTAGTAAACCTGTATGTCATTTCCTTGGAAATAAGGCTATTTTATGGGTACCTAATTTAGGTTTGGACAATGCCTATGGTGCCAATGTTGCTGTTTAG
Protein sequence

>Msa0296270

MGFHHLIIFISVLSLALAGESHASLPEEEYWEAVWPNTPIPTSLRELLKPGPQGVEIDDLPMEIDDTQYPKAFFYQHELYPGKSMNVQFSKRPFAQPYGVYTWMREIKDIEKEGYTFNEVCVKKAAAEGEQKFCAKSLGTLIGFAISKLGKNIQALSSSFIDKHEQYKIESVQNLGEKAVMCHRLNFQKVVFYCHEIHGTTAFMVPLVANDGTKTQALAVCHADTSGMNHEMLQQIMKADPGSKPVCHFLGNKAILWVPNLGLDNAYGANVAV*