AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300100 MtrunA17_Chr2g0324821 70.940 117 33 1 194 310 18 133 4.86e-52 168
Msa0300100 MtrunA17_Chr2g0325861 56.021 191 70 1 1 191 421 597 3.56e-49 175
Msa0300100 MtrunA17_Chr2g0325861 51.075 186 91 0 1 186 337 522 4.61e-37 140
Msa0300100 MtrunA17_Chr2g0325861 35.333 300 150 7 1 280 463 738 1.90e-28 115
Msa0300100 MtrunA17_Chr2g0325861 43.448 145 82 0 42 186 294 438 5.70e-17 81.6
Msa0300100 MtrunA17_Chr2g0325361 54.545 198 82 2 1 191 478 674 1.62e-46 168
Msa0300100 MtrunA17_Chr2g0325361 48.611 216 69 4 1 188 562 763 2.32e-38 144
Msa0300100 MtrunA17_Chr2g0325361 47.849 186 97 0 1 186 520 705 4.93e-37 140
Msa0300100 MtrunA17_Chr2g0325361 44.498 209 91 3 3 186 420 628 9.26e-33 128
Msa0300100 MtrunA17_Chr2g0325361 41.667 204 87 2 15 186 320 523 5.44e-29 117
Msa0300100 MtrunA17_Chr2g0325361 36.364 187 105 1 13 185 262 448 1.68e-18 86.3
Msa0300100 MtrunA17_Chr2g0325361 30.892 314 173 8 1 287 604 900 8.50e-17 81.3
Msa0300100 MtrunA17_Chr2g0325361 40.000 160 88 2 42 194 263 421 1.03e-14 75.1
Msa0300100 MtrunA17_Chr2g0325691 56.216 185 80 1 2 186 307 490 1.75e-43 158
Msa0300100 MtrunA17_Chr2g0325691 47.170 212 100 3 6 206 223 433 4.63e-32 125
Msa0300100 MtrunA17_Chr2g0325691 42.917 240 84 4 1 188 348 586 9.78e-31 122
Msa0300100 MtrunA17_Chr2g0325691 43.662 213 119 1 1 213 518 729 9.22e-30 119
Msa0300100 MtrunA17_Chr2g0325691 43.541 209 107 3 1 206 473 673 7.64e-28 114
Msa0300100 MtrunA17_Chr2g0325691 38.249 217 88 7 17 194 198 407 1.05e-16 80.9
Msa0300100 MtrunA17_Chr2g0325281 49.020 204 96 2 1 198 380 581 3.02e-41 151
Msa0300100 MtrunA17_Chr2g0325281 41.579 190 111 0 1 190 254 443 1.72e-29 118
Msa0300100 MtrunA17_Chr2g0325281 46.575 146 64 1 1 146 464 595 6.92e-21 93.2
Msa0300100 MtrunA17_Chr2g0325281 40.800 125 60 1 55 179 252 362 3.88e-13 70.1
Msa0300100 MtrunA17_Chr2g0325321 50.267 187 93 0 1 187 380 566 7.62e-41 151
Msa0300100 MtrunA17_Chr2g0325321 39.062 192 103 1 13 190 252 443 5.18e-22 96.7
Msa0300100 MtrunA17_Chr2g0325321 47.107 121 50 1 1 107 464 584 1.67e-17 83.2
Msa0300100 MtrunA17_Chr2g0282661 57.724 123 49 2 190 310 24 145 5.46e-39 135
Msa0300100 MtrunA17_Chr5g0413571 54.676 139 58 2 176 314 1 134 5.97e-39 134
Msa0300100 MtrunA17_Chr7g0235901 53.933 178 78 1 6 179 132 309 6.37e-39 139
Msa0300100 MtrunA17_Chr7g0235901 40.132 152 77 1 1 138 159 310 4.08e-18 83.2
Msa0300100 MtrunA17_Chr7g0235901 47.423 97 37 1 1 83 215 311 2.71e-13 69.3
Msa0300100 MtrunA17_Chr2g0325231 48.585 212 90 4 1 194 346 556 9.53e-36 137
Msa0300100 MtrunA17_Chr2g0325231 49.761 209 93 3 6 203 221 428 4.46e-35 135
Msa0300100 MtrunA17_Chr2g0325231 33.618 351 171 9 1 292 437 784 1.01e-29 119
Msa0300100 MtrunA17_Chr2g0325231 32.209 326 185 8 1 294 497 818 6.89e-27 111
Msa0300100 MtrunA17_Chr2g0325231 44.094 127 59 3 91 206 222 347 5.64e-11 63.5
Msa0300100 MtrunA17_Chr2g0325641 42.353 255 85 4 1 193 29 283 2.36e-35 130
Msa0300100 MtrunA17_Chr2g0325641 41.209 182 102 3 15 194 1 179 1.35e-19 87.8
Msa0300100 MtrunA17_Chr2g0325641 43.791 153 79 1 1 146 162 314 1.89e-19 87.4
Msa0300100 MtrunA17_Chr2g0325641 38.690 168 89 3 3 156 143 310 1.50e-15 76.3
Msa0300100 MtrunA17_Chr2g0325641 33.668 199 109 5 3 186 115 305 1.70e-13 70.1
Msa0300100 MtrunA17_Chr2g0325761 44.550 211 101 3 1 202 376 579 3.50e-29 117
Msa0300100 MtrunA17_Chr2g0325761 39.316 234 113 3 1 206 264 496 3.16e-25 105
Msa0300100 MtrunA17_Chr2g0325761 45.625 160 66 2 1 153 446 591 2.94e-24 103
Msa0300100 MtrunA17_Chr2g0325761 40.476 168 93 1 14 181 431 591 3.42e-21 94.4
Msa0300100 MtrunA17_Chr2g0325761 36.979 192 101 4 3 175 220 410 2.49e-17 82.8
Msa0300100 MtrunA17_Chr5g0416271 33.448 290 140 5 1 289 173 410 2.50e-28 114
Msa0300100 MtrunA17_Chr5g0416271 38.889 144 51 4 86 194 147 288 6.97e-12 65.9
Msa0300100 MtrunA17_Chr2g0325241 36.122 263 134 6 17 246 143 404 1.79e-26 108
Msa0300100 MtrunA17_Chr2g0325241 39.157 166 87 1 22 187 120 271 1.23e-21 94.7
Msa0300100 MtrunA17_Chr2g0325221 38.500 200 109 1 1 186 313 512 1.65e-25 107
Msa0300100 MtrunA17_Chr2g0325221 39.247 186 105 2 13 191 311 495 1.79e-21 95.1
Msa0300100 MtrunA17_Chr2g0325221 34.404 218 119 2 1 194 383 600 6.42e-18 84.3
Msa0300100 MtrunA17_Chr2g0325661 32.448 339 161 8 1 289 381 701 1.97e-25 106
Msa0300100 MtrunA17_Chr2g0325661 39.623 212 100 2 1 184 269 480 1.64e-22 98.2
Msa0300100 MtrunA17_Chr2g0325661 32.828 198 109 4 3 186 225 412 3.83e-13 70.1
Msa0300100 MtrunA17_Chr2g0325721 49.133 173 88 0 1 173 329 501 5.38e-25 104
Msa0300100 MtrunA17_Chr2g0325721 42.105 190 110 0 1 190 287 476 4.16e-19 87.8
Msa0300100 MtrunA17_Chr2g0325721 40.000 180 86 4 22 194 280 444 4.78e-13 69.7
Msa0300100 MtrunA17_Chr2g0325711 37.327 217 105 3 1 186 279 495 1.03e-24 104
Msa0300100 MtrunA17_Chr2g0325711 29.467 319 180 6 2 285 350 658 3.02e-22 97.4
Msa0300100 MtrunA17_Chr2g0325711 35.714 182 110 1 12 186 276 457 4.94e-20 90.9
Msa0300100 MtrunA17_Chr2g0325651 47.059 153 71 2 15 160 236 385 1.21e-24 102
Msa0300100 MtrunA17_Chr2g0325651 40.385 156 64 3 43 191 236 369 6.23e-17 80.9
Msa0300100 MtrunA17_Chr5g0434751 38.739 222 101 2 1 187 15 236 1.54e-24 100
Msa0300100 MtrunA17_Chr5g0434751 40.860 186 82 3 29 186 1 186 4.84e-20 88.6
Msa0300100 MtrunA17_Chr5g0434751 44.366 142 72 1 1 135 127 268 2.65e-14 72.0
Msa0300100 MtrunA17_Chr5g0434751 33.010 206 103 2 1 171 57 262 2.52e-12 66.2
Msa0300100 MtrunA17_Chr2g0325701 42.697 178 98 1 6 179 234 411 1.77e-24 103
Msa0300100 MtrunA17_Chr2g0325701 34.591 159 89 2 4 162 278 421 3.54e-14 73.2
Msa0300100 MtrunA17_Chr2g0325701 35.484 217 113 7 2 195 137 349 1.68e-13 70.9
Msa0300100 MtrunA17_Chr2g0280141 39.062 192 110 1 2 186 260 451 6.93e-23 99.4
Msa0300100 MtrunA17_Chr2g0280141 39.252 214 102 4 1 186 252 465 7.61e-23 99.0
Msa0300100 MtrunA17_Chr2g0280141 38.172 186 114 1 1 186 308 492 2.11e-21 94.7
Msa0300100 MtrunA17_Chr2g0280141 29.139 302 177 6 1 281 350 635 4.58e-21 94.0
Msa0300100 MtrunA17_Chr2g0280141 38.286 175 86 4 42 195 251 424 2.43e-16 79.7
Msa0300100 MtrunA17_Chr6g0476691 55.844 77 34 0 234 310 17 93 4.80e-22 89.0
Msa0300100 MtrunA17_Chr2g0325811 37.073 205 105 2 1 181 365 569 2.13e-21 94.7
Msa0300100 MtrunA17_Chr2g0325811 33.937 221 100 4 1 190 309 514 4.26e-21 94.0
Msa0300100 MtrunA17_Chr2g0325811 30.723 166 73 1 50 173 302 467 3.79e-11 63.9
Msa0300100 MtrunA17_Chr2g0325351 29.139 302 178 7 6 289 230 513 1.77e-20 92.0
Msa0300100 MtrunA17_Chr2g0325351 39.007 141 54 3 2 110 261 401 8.19e-11 62.8
Msa0300100 MtrunA17_Chr6g0471251 37.949 195 86 3 6 186 270 443 1.62e-19 89.4
Msa0300100 MtrunA17_Chr6g0471251 30.405 296 145 7 1 281 335 584 1.88e-13 70.9
Msa0300100 MtrunA17_Chr2g0325611 31.683 303 172 9 7 289 128 415 7.74e-19 87.0
Msa0300100 MtrunA17_Chr8g0365411 39.259 135 44 1 176 310 1 97 1.12e-18 79.7
Msa0300100 MtrunA17_Chr2g0325781 55.682 88 35 1 91 174 215 302 4.51e-18 83.2
Msa0300100 MtrunA17_Chr2g0325621 46.923 130 69 0 42 171 248 377 7.18e-18 84.3
Msa0300100 MtrunA17_Chr2g0325621 39.568 139 84 0 1 139 249 387 3.91e-14 73.2
Msa0300100 MtrunA17_Chr2g0325731 67.241 58 19 0 113 170 1 58 4.44e-17 74.7
Msa0300100 MtrunA17_Chr2g0325731 56.452 62 27 0 29 90 1 62 6.35e-13 63.2
Msa0300100 MtrunA17_Chr2g0325791 47.009 117 58 1 6 118 41 157 1.50e-15 76.3
Msa0300100 MtrunA17_Chr2g0325791 30.460 174 104 5 69 229 41 210 5.61e-12 65.5
Msa0300100 MtrunA17_Chr2g0325791 38.346 133 77 2 30 158 24 155 5.93e-12 65.5
Msa0300100 MtrunA17_Chr2g0325751 40.909 132 71 1 57 188 7 131 1.78e-14 70.1
Msa0300100 MtrunA17_Chr2g0325751 41.129 124 66 1 15 138 7 123 1.78e-13 67.0
Msa0300100 MtrunA17_Chr2g0325751 37.984 129 73 1 24 152 2 123 8.57e-13 65.1
Msa0300100 MtrunA17_Chr2g0325751 44.898 98 54 0 2 99 29 126 5.92e-12 62.8
Msa0300100 MtrunA17_Chr2g0325751 37.903 124 70 1 1 124 7 123 3.23e-11 60.8
Msa0300100 MtrunA17_Chr2g0333341 39.552 134 66 2 43 176 332 450 1.80e-14 73.9
Msa0300100 MtrunA17_Chr2g0333341 39.850 133 65 2 1 133 332 449 6.33e-13 69.3
Msa0300100 MtrunA17_Chr5g0438061 36.434 129 66 5 190 316 107 221 1.58e-13 68.9
Msa0300100 MtrunA17_Chr2g0325261 44.706 85 47 0 29 113 222 306 3.70e-11 63.2
Msa0300100 MtrunA17_Chr2g0325341 44.706 85 47 0 29 113 222 306 3.70e-11 63.2
Msa0300100 MtrunA17_Chr5g0438041 34.884 129 66 5 193 316 12 127 8.54e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300100 AT2G33845.1 60.976 123 40 2 190 310 59 175 3.63e-41 142
Msa0300100 AT4G28440.1 61.345 119 43 2 194 310 28 145 4.24e-41 140
Msa0300100 AT1G23750.1 54.167 120 50 2 190 309 16 130 1.35e-37 131
Msa0300100 AT1G03810.1 57.851 121 49 1 194 314 25 143 2.95e-36 127
Msa0300100 AT1G10590.2 49.640 139 66 2 176 313 1 136 4.50e-35 124
Msa0300100 AT1G10590.1 49.640 139 66 2 176 313 1 136 4.50e-35 124
Msa0300100 AT1G10590.3 49.286 140 67 2 175 313 14 150 1.14e-34 124
Msa0300100 AT1G23750.2 66.667 81 27 0 229 309 13 93 4.64e-33 118

Find 133 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCATCCGGTCTTCATTT+TGG 0.147230 2_3:-55437495 None:intergenic
ATGCAAAGATTAACCTGTTC+TGG 0.253388 2_3:+55439008 Msa0300100:CDS
TGGTGTCGAATATTATGATT+AGG 0.284068 2_3:+55436583 Msa0300100:five_prime_UTR
TTGATTTAAACTCCTTCATT+TGG 0.300483 2_3:-55437906 None:intergenic
TGCTTTCGGTGTGGTCAATA+TGG 0.314123 2_3:+55436648 Msa0300100:five_prime_UTR
TCAATACCAGCTCCTTCATT+TGG 0.329113 2_3:-55437423 None:intergenic
TCTCTAACTTCAAAACTTGC+AGG 0.345507 2_3:-55439082 None:intergenic
TCAAGGATAGGATCAACTTT+GGG 0.349068 2_3:+55436483 None:intergenic
CATTTCTAAACAAAAGCATT+TGG 0.354274 2_3:+55436857 Msa0300100:five_prime_UTR
GATAGATTTGGATGGATGTT+TGG 0.365545 2_3:+55436563 Msa0300100:five_prime_UTR
GGTGTCGAATATTATGATTA+GGG 0.380228 2_3:+55436584 Msa0300100:five_prime_UTR
AGAGTGTGTCCATCAGTTCC+TGG 0.381134 2_3:-55437970 None:intergenic
GTTGCGAGCTGTGAAGATTA+TGG 0.381200 2_3:-55438118 None:intergenic
TCCTTTCAATTGCATCTCTT+CGG 0.386064 2_3:-55437863 None:intergenic
TCCCGTCAAGTCCTTCATAT+TGG 0.391697 2_3:-55436623 None:intergenic
TGAAAGCAAAATGAAGAAAC+TGG 0.395480 2_3:+55437669 Msa0300100:CDS
CTTCACAGCTCGCAACGAAC+AGG 0.397474 2_3:+55438125 Msa0300100:CDS
TCGACTCAAGTTCCTTCATT+CGG 0.398246 2_3:-55437076 None:intergenic
GATAGATTTGGTGACTGATT+TGG 0.398420 2_3:+55437028 Msa0300100:five_prime_UTR
TGAGGCTGGCAGTTGATTGC+CGG 0.400616 2_3:+55439038 Msa0300100:CDS
CGGATTTGAGCTCCTCCACC+TGG 0.402176 2_3:-55437843 None:intergenic
TGAAGGTCAAGTGATAGATT+TGG 0.402956 2_3:+55437016 Msa0300100:five_prime_UTR
TTGAGTCATGCTCCTTCATC+CGG 0.403682 2_3:-55437507 None:intergenic
TCGACTCAAGGTCCTTCATT+CGG 0.407601 2_3:-55437214 None:intergenic
TGCCTAGTTGGAGACGAAAC+TGG 0.409862 2_3:+55438093 Msa0300100:CDS
GACTTGACGGGATATGCTTT+CGG 0.414906 2_3:+55436634 Msa0300100:five_prime_UTR
GCCTCATATGTCCCCAGAAC+AGG 0.421872 2_3:-55439021 None:intergenic
TAATGTTGAAGGTTGATGAT+AGG 0.428079 2_3:+55436508 Msa0300100:five_prime_UTR
GGAAGTAGAGCTATGTTTGA+TGG 0.432924 2_3:+55436764 Msa0300100:five_prime_UTR
TCAACTCAAACATCTTCATC+TGG 0.434798 2_3:-55437717 None:intergenic
CTCAAGGATAGGATCAACTT+TGG 0.441059 2_3:+55436482 None:intergenic
GGCATTCAGCGATGCGGGAG+GGG 0.443427 2_3:-55438074 None:intergenic
TTGATCTGTGCTCCTTCACC+CGG 0.444119 2_3:-55437255 None:intergenic
TGCAAAGATTAACCTGTTCT+GGG 0.444397 2_3:+55439009 Msa0300100:CDS
AGACGGCCATTCATCGCGGC+AGG 0.444865 2_3:-55438034 None:intergenic
CATTCACCAGCTCCTTCACT+TGG 0.448226 2_3:-55436884 None:intergenic
CGCATCGCTGAATGCCTAGT+TGG 0.457979 2_3:+55438081 Msa0300100:CDS
CTCCAATATGAAGGACTTGA+CGG 0.458749 2_3:+55436621 Msa0300100:five_prime_UTR
CCTTTGTTTGGCCTTTGAGT+TGG 0.459817 2_3:-55437621 None:intergenic
ACCTGTTCTGGGGACATATG+AGG 0.469408 2_3:+55439020 Msa0300100:CDS
ACGGCCATTCATCGCGGCAG+GGG 0.470283 2_3:-55438032 None:intergenic
TCGATTCATGCTCCTCTACC+CGG 0.471523 2_3:-55437118 None:intergenic
TGAAAGTAAAGTGGAGGAGC+TGG 0.471794 2_3:+55437795 Msa0300100:CDS
TGAAAGGCAAGTTAAGGAGT+TGG 0.472552 2_3:+55437333 Msa0300100:five_prime_UTR
AATATGTGTGGAGATAGATT+TGG 0.473353 2_3:+55436551 Msa0300100:five_prime_UTR
ATTGCATTTCTTTCGACTCA+AGG 0.473853 2_3:-55437226 None:intergenic
AACTAGGCATTCAGCGATGC+GGG 0.477868 2_3:-55438079 None:intergenic
TGAAGTTTGCGAGAAAATGC+AGG 0.484103 2_3:+55436704 Msa0300100:five_prime_UTR
GGACAAATTAAAGAATTTGA+AGG 0.485990 2_3:+55437883 Msa0300100:CDS
GAGGCTGGCAGTTGATTGCC+GGG 0.487781 2_3:+55439039 Msa0300100:CDS
GCAACATGAAGGCCGGGTAG+AGG 0.489755 2_3:+55437106 Msa0300100:five_prime_UTR
AGAATTTGAAGGCCAAATGA+AGG 0.491861 2_3:+55437894 Msa0300100:CDS
CGCAGAATTACAGTAGCACC+AGG 0.493091 2_3:-55438983 None:intergenic
GCAATTCGAGAGCGGAATGA+AGG 0.499417 2_3:+55436926 Msa0300100:five_prime_UTR
TGCAATATGAAGCCGGGTGA+AGG 0.501672 2_3:+55437243 Msa0300100:five_prime_UTR
TAGAGAATTTGAAAGTAAAG+TGG 0.505463 2_3:+55437786 Msa0300100:CDS
CACAAGAAAGGTAATTGTGT+TGG 0.509056 2_3:+55436672 Msa0300100:five_prime_UTR
CTGGTTTCAGATCTATCACT+CGG 0.510842 2_3:-55437951 None:intergenic
GAAGGAGCTGGTGAATGAAT+TGG 0.514445 2_3:+55436890 Msa0300100:five_prime_UTR
AAGAGAAGCAACATGAAGGC+CGG 0.515329 2_3:+55437099 Msa0300100:five_prime_UTR
AGAGAAGCAACATGAAGGTC+GGG 0.518749 2_3:+55437741 Msa0300100:CDS
CGAGTTAATCTCCAATATGA+AGG 0.519768 2_3:+55436612 Msa0300100:five_prime_UTR
AAGGTTGATGATAGGCTAAG+TGG 0.520158 2_3:+55436516 Msa0300100:five_prime_UTR
TCAAAGAAGCGAGAATTTGA+AGG 0.520665 2_3:+55437526 Msa0300100:CDS
TTCCAGTTTCGTCTCCAACT+AGG 0.523242 2_3:-55438095 None:intergenic
TGTTTGATGGAGGACTTGAT+TGG 0.525108 2_3:+55436777 Msa0300100:five_prime_UTR
CCAACTTGTAAGTAAAATGA+AGG 0.525826 2_3:+55437369 Msa0300100:exon
AGAGAAGCAGCATGAAGGTC+GGG 0.525926 2_3:+55436962 Msa0300100:five_prime_UTR
AAAGAAATGCAATATGAAGC+CGG 0.526151 2_3:+55437236 Msa0300100:five_prime_UTR
GCTGTTTGAAAGGCAAGTTA+AGG 0.528403 2_3:+55437327 Msa0300100:five_prime_UTR
CCAAAATGAAGACCGGATGA+AGG 0.534699 2_3:+55437495 Msa0300100:CDS
AGGCATTCAGCGATGCGGGA+GGG 0.535234 2_3:-55438075 None:intergenic
TTGACTCAAACAGTTTGACT+TGG 0.537417 2_3:-55437591 None:intergenic
GATCTGAAACCAGGAACTGA+TGG 0.538419 2_3:+55437961 Msa0300100:CDS
AGAATTCGAAAGCCAAATGA+AGG 0.543820 2_3:+55437411 Msa0300100:CDS
GCAGCATGAAGGTCGGGTGA+AGG 0.550368 2_3:+55436968 Msa0300100:five_prime_UTR
CTAAACAGAAGCTGTTTGAA+AGG 0.550931 2_3:+55437317 Msa0300100:five_prime_UTR
TCCAATATGAAGGACTTGAC+GGG 0.554496 2_3:+55436622 Msa0300100:five_prime_UTR
GAAGGTTGGTTGCTGTTCAT+CGG 0.555268 2_3:+55437999 Msa0300100:CDS
CGAAAGCCAAATGAAGGAGC+TGG 0.557116 2_3:+55437417 Msa0300100:CDS
CGGCCATTCATCGCGGCAGG+GGG 0.557172 2_3:-55438031 None:intergenic
AGAATTTGAATGTCAAGTGA+AGG 0.557857 2_3:+55437285 Msa0300100:five_prime_UTR
CTGTGAGCTGTATACATCCC+CGG 0.558440 2_3:-55439057 None:intergenic
GCATTTCGAAAGTCGAATGA+AGG 0.559949 2_3:+55437453 Msa0300100:CDS
TCAAATTATAACCAACTCAA+AGG 0.560582 2_3:+55437610 Msa0300100:CDS
AGAGAAGCAACATGAAGGCC+GGG 0.561927 2_3:+55437100 Msa0300100:five_prime_UTR
GACGGCCATTCATCGCGGCA+GGG 0.563301 2_3:-55438033 None:intergenic
AAGAGAACCAAAATGAAGAC+CGG 0.565006 2_3:+55437488 Msa0300100:CDS
AAGAAATGCAATATGAAGCC+GGG 0.565633 2_3:+55437237 Msa0300100:five_prime_UTR
AGGACTTGATTGGAGAGTGC+AGG 0.568670 2_3:+55436787 Msa0300100:five_prime_UTR
AGGTTGATGATAGGCTAAGT+GGG 0.570548 2_3:+55436517 Msa0300100:five_prime_UTR
CGAGTGATAGATCTGAAACC+AGG 0.572545 2_3:+55437952 Msa0300100:CDS
TCCGAAGAGATGCAATTGAA+AGG 0.572726 2_3:+55437862 Msa0300100:CDS
CTTGATTGGAGAGTGCAGGC+AGG 0.579042 2_3:+55436791 Msa0300100:five_prime_UTR
GCAACATGAAGGTCGGGTGA+AGG 0.579309 2_3:+55437747 Msa0300100:CDS
TAAGATTGAAGAGTTTGTCG+AGG 0.580976 2_3:+55436728 Msa0300100:five_prime_UTR
GGTCAATATGGACACAAGAA+AGG 0.584743 2_3:+55436660 Msa0300100:five_prime_UTR
TCGAAAGAGAAGCAACATGA+AGG 0.592048 2_3:+55437095 Msa0300100:five_prime_UTR
AAGAGAAGCAACATGAAGGT+CGG 0.592279 2_3:+55437740 Msa0300100:CDS
GATGGAGCTTTATCAAGTCA+TGG 0.592913 2_3:+55436830 Msa0300100:five_prime_UTR
GTGTCGAATATTATGATTAG+GGG 0.593607 2_3:+55436585 Msa0300100:five_prime_UTR
TTGAAAGAGAAGCAACATGA+AGG 0.594016 2_3:+55437736 Msa0300100:CDS
GTTCTGGGGACATATGAGGC+TGG 0.595747 2_3:+55439024 Msa0300100:CDS
GGAAAGCCAAGTGAAGGAGC+TGG 0.596353 2_3:+55436878 Msa0300100:five_prime_UTR
CAAGGATAGGATCAACTTTG+GGG 0.597510 2_3:+55436484 None:intergenic
CGAGCTGAAGCTATCAAAGA+CGG 0.598648 2_3:-55438051 None:intergenic
AGAATTTGAAGGTCAAGTGA+AGG 0.598860 2_3:+55437537 Msa0300100:CDS
AGAATTTGAAAGTAAAGTGG+AGG 0.600864 2_3:+55437789 Msa0300100:CDS
GAAGTTTGCGAGAAAATGCA+GGG 0.605272 2_3:+55436705 Msa0300100:five_prime_UTR
TAGGCATTCAGCGATGCGGG+AGG 0.606084 2_3:-55438076 None:intergenic
AAATCACTCTTCAACGACAT+CGG 0.606854 2_3:-55439147 None:intergenic
AAGAGAAGCAGCATGAAGGT+CGG 0.608292 2_3:+55436961 Msa0300100:five_prime_UTR
GCATTTGGAAAGCCAAGTGA+AGG 0.609390 2_3:+55436872 Msa0300100:five_prime_UTR
TTAAAAGAGAAGCAGCATGA+AGG 0.610849 2_3:+55436957 Msa0300100:five_prime_UTR
TTCACAGCTCGCAACGAACA+GGG 0.612349 2_3:+55438126 Msa0300100:CDS
CAGTAGCACCAGGTTTCATG+AGG 0.614066 2_3:-55438973 None:intergenic
AGAATTTAAAGATCATGTGA+CGG 0.619537 2_3:+55437148 Msa0300100:five_prime_UTR
TGAATGTCAAGTGAAGGAGC+AGG 0.620944 2_3:+55437291 Msa0300100:five_prime_UTR
CACACTCTGACAGTGAAGGT+TGG 0.635603 2_3:+55437985 Msa0300100:CDS
GCAGTGGACCTCATGAAACC+TGG 0.636118 2_3:+55438965 Msa0300100:intron
CAACTAGGCATTCAGCGATG+CGG 0.638058 2_3:-55438080 None:intergenic
AGTAGAGCTATGTTTGATGG+AGG 0.642585 2_3:+55436767 Msa0300100:five_prime_UTR
GACGGGATATGCTTTCGGTG+TGG 0.660721 2_3:+55436639 Msa0300100:five_prime_UTR
GCAAAGATTAACCTGTTCTG+GGG 0.661250 2_3:+55439010 Msa0300100:CDS
TGTCGAATATTATGATTAGG+GGG 0.661343 2_3:+55436586 Msa0300100:five_prime_UTR
AGACAAAAGCAATTCGAGAG+CGG 0.666493 2_3:+55436918 Msa0300100:five_prime_UTR
GAAATACGCGAGAATATGTG+TGG 0.672119 2_3:+55436539 Msa0300100:five_prime_UTR
TGGACACACTCTGACAGTGA+AGG 0.677324 2_3:+55437981 Msa0300100:CDS
TGTGTGGAGATAGATTTGGA+TGG 0.680421 2_3:+55436555 Msa0300100:five_prime_UTR
CCAACTCAAAGGCCAAACAA+AGG 0.684873 2_3:+55437621 Msa0300100:CDS
AAGGATAGGATCAACTTTGG+GGG 0.707340 2_3:+55436485 None:intergenic
AGGCTGGCAGTTGATTGCCG+GGG 0.718353 2_3:+55439040 Msa0300100:CDS
TCAAAGACGGCCATTCATCG+CGG 0.726292 2_3:-55438038 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAAAATAGAGAATTTGA+AGG + chr2_3:55436999-55437018 Msa0300100:five_prime_UTR 20.0%
!!! TAAAACTCGTTGATTTTATA+TGG + chr2_3:55438387-55438406 Msa0300100:intron 20.0%
!!! ACCTAATTTTATGCATATTA+TGG + chr2_3:55438444-55438463 Msa0300100:intron 20.0%
!! TCCATAATATGCATAAAATT+AGG - chr2_3:55438448-55438467 None:intergenic 20.0%
!!! AACATATGGGTTTAATTTAT+CGG + chr2_3:55438738-55438757 Msa0300100:intron 20.0%
!!! CATTTCTAAACAAAAGCATT+TGG + chr2_3:55436857-55436876 Msa0300100:five_prime_UTR 25.0%
! AGAATTTAAAGATCATGTGA+CGG + chr2_3:55437148-55437167 Msa0300100:five_prime_UTR 25.0%
! TCAAATTATAACCAACTCAA+AGG + chr2_3:55437610-55437629 Msa0300100:CDS 25.0%
! TTAAAAAGAACTCCTTTGTT+TGG - chr2_3:55437636-55437655 None:intergenic 25.0%
! TAGAGAATTTGAAAGTAAAG+TGG + chr2_3:55437786-55437805 Msa0300100:CDS 25.0%
! GGACAAATTAAAGAATTTGA+AGG + chr2_3:55437883-55437902 Msa0300100:CDS 25.0%
! TTGATTTAAACTCCTTCATT+TGG - chr2_3:55437909-55437928 None:intergenic 25.0%
! ATGTTCAATTTAAGTGCTAT+TGG + chr2_3:55438195-55438214 Msa0300100:intron 25.0%
!!! GATATGTAATTTGTTGACTT+GGG + chr2_3:55438217-55438236 Msa0300100:intron 25.0%
!!! AAGACTTGAAAGTGTTTTTA+AGG + chr2_3:55438280-55438299 Msa0300100:intron 25.0%
! TACATTTACACAATCAATGA+AGG - chr2_3:55438347-55438366 None:intergenic 25.0%
!! TTCATTGATTGTGTAAATGT+AGG + chr2_3:55438346-55438365 Msa0300100:intron 25.0%
! TATTATTACTGCATTCAAGT+AGG + chr2_3:55438477-55438496 Msa0300100:intron 25.0%
! TAAAGAGTGATATTGTCTTA+TGG + chr2_3:55438516-55438535 Msa0300100:intron 25.0%
! TATTCAGCAGTTAAACATAT+GGG + chr2_3:55438725-55438744 Msa0300100:intron 25.0%
!! TTTAATTTATCGGATGGTAT+AGG + chr2_3:55438748-55438767 Msa0300100:intron 25.0%
! AAAGAACAAAATATGTGCTA+AGG - chr2_3:55438878-55438897 None:intergenic 25.0%
!!! ATTCATTTACTTTTATGCAG+TGG + chr2_3:55438949-55438968 Msa0300100:intron 25.0%
!! TAATGTTGAAGGTTGATGAT+AGG + chr2_3:55436508-55436527 Msa0300100:five_prime_UTR 30.0%
AATATGTGTGGAGATAGATT+TGG + chr2_3:55436551-55436570 Msa0300100:five_prime_UTR 30.0%
! TGGTGTCGAATATTATGATT+AGG + chr2_3:55436583-55436602 Msa0300100:five_prime_UTR 30.0%
! GGTGTCGAATATTATGATTA+GGG + chr2_3:55436584-55436603 Msa0300100:five_prime_UTR 30.0%
GTGTCGAATATTATGATTAG+GGG + chr2_3:55436585-55436604 Msa0300100:five_prime_UTR 30.0%
TGTCGAATATTATGATTAGG+GGG + chr2_3:55436586-55436605 Msa0300100:five_prime_UTR 30.0%
GGAATTTGAGAAAAAAGAGA+TGG + chr2_3:55436812-55436831 Msa0300100:five_prime_UTR 30.0%
AAAGAAATGCAATATGAAGC+CGG + chr2_3:55437236-55437255 Msa0300100:five_prime_UTR 30.0%
AGAATTTGAATGTCAAGTGA+AGG + chr2_3:55437285-55437304 Msa0300100:five_prime_UTR 30.0%
! CCTTCATTTTACTTACAAGT+TGG - chr2_3:55437372-55437391 None:intergenic 30.0%
CCAACTTGTAAGTAAAATGA+AGG + chr2_3:55437369-55437388 Msa0300100:exon 30.0%
!! AGGAGTTCTTTTTAAAAGAG+AGG + chr2_3:55437641-55437660 Msa0300100:CDS 30.0%
!! GGAGTTCTTTTTAAAAGAGA+GGG + chr2_3:55437642-55437661 Msa0300100:CDS 30.0%
TGAAAGCAAAATGAAGAAAC+TGG + chr2_3:55437669-55437688 Msa0300100:CDS 30.0%
AGAATTTGAAAGTAAAGTGG+AGG + chr2_3:55437789-55437808 Msa0300100:CDS 30.0%
AAACACAATGCAACAGTTAA+AGG - chr2_3:55438175-55438194 None:intergenic 30.0%
!! GGATATGTAATTTGTTGACT+TGG + chr2_3:55438216-55438235 Msa0300100:intron 30.0%
AAGTCTTAACTGCTTAGAAA+TGG - chr2_3:55438267-55438286 None:intergenic 30.0%
TATGCATAAAATTAGGTCCA+GGG - chr2_3:55438441-55438460 None:intergenic 30.0%
ATATGCATAAAATTAGGTCC+AGG - chr2_3:55438442-55438461 None:intergenic 30.0%
! CTGGATAAGCTTTATGTAAT+GGG + chr2_3:55438581-55438600 Msa0300100:intron 30.0%
TCAATGACATCTAACGAATA+TGG - chr2_3:55438697-55438716 None:intergenic 30.0%
CATATGTTTAACTGCTGAAT+AGG - chr2_3:55438726-55438745 None:intergenic 30.0%
CTATTCAGCAGTTAAACATA+TGG + chr2_3:55438724-55438743 Msa0300100:intron 30.0%
! TATGGGTTTAATTTATCGGA+TGG + chr2_3:55438742-55438761 Msa0300100:intron 30.0%
AAGATAAAATGTAACAGCCA+GGG - chr2_3:55438845-55438864 None:intergenic 30.0%
ACAAAATATGTGCTAAGGAA+TGG - chr2_3:55438873-55438892 None:intergenic 30.0%
!!! CTTGTTTTTGAAGAGGATAT+CGG + chr2_3:55438918-55438937 Msa0300100:intron 30.0%
GATAGATTTGGATGGATGTT+TGG + chr2_3:55436563-55436582 Msa0300100:five_prime_UTR 35.0%
CGAGTTAATCTCCAATATGA+AGG + chr2_3:55436612-55436631 Msa0300100:five_prime_UTR 35.0%
CACAAGAAAGGTAATTGTGT+TGG + chr2_3:55436672-55436691 Msa0300100:five_prime_UTR 35.0%
TAAGATTGAAGAGTTTGTCG+AGG + chr2_3:55436728-55436747 Msa0300100:five_prime_UTR 35.0%
TTAAAAGAGAAGCAGCATGA+AGG + chr2_3:55436957-55436976 Msa0300100:five_prime_UTR 35.0%
TGAAGGTCAAGTGATAGATT+TGG + chr2_3:55437016-55437035 Msa0300100:five_prime_UTR 35.0%
!! GATAGATTTGGTGACTGATT+TGG + chr2_3:55437028-55437047 Msa0300100:five_prime_UTR 35.0%
ATTGCATTTCTTTCGACTCA+AGG - chr2_3:55437229-55437248 None:intergenic 35.0%
AAGAAATGCAATATGAAGCC+GGG + chr2_3:55437237-55437256 Msa0300100:five_prime_UTR 35.0%
CTAAACAGAAGCTGTTTGAA+AGG + chr2_3:55437317-55437336 Msa0300100:five_prime_UTR 35.0%
AGAATTCGAAAGCCAAATGA+AGG + chr2_3:55437411-55437430 Msa0300100:CDS 35.0%
AAGAGAACCAAAATGAAGAC+CGG + chr2_3:55437488-55437507 Msa0300100:CDS 35.0%
TCAAAGAAGCGAGAATTTGA+AGG + chr2_3:55437526-55437545 Msa0300100:CDS 35.0%
AGAATTTGAAGGTCAAGTGA+AGG + chr2_3:55437010-55437029 Msa0300100:five_prime_UTR 35.0%
TTGACTCAAACAGTTTGACT+TGG - chr2_3:55437594-55437613 None:intergenic 35.0%
! GTGAAAAAGAGACGTTTTGA+AGG + chr2_3:55437694-55437713 Msa0300100:CDS 35.0%
TCAACTCAAACATCTTCATC+TGG - chr2_3:55437720-55437739 None:intergenic 35.0%
TTGAAAGAGAAGCAACATGA+AGG + chr2_3:55437736-55437755 Msa0300100:CDS 35.0%
!! AAAGATGCATTTTGAAAGCC+AGG + chr2_3:55437825-55437844 Msa0300100:CDS 35.0%
TCCTTTCAATTGCATCTCTT+CGG - chr2_3:55437866-55437885 None:intergenic 35.0%
AGAATTTGAAGGCCAAATGA+AGG + chr2_3:55437894-55437913 Msa0300100:CDS 35.0%
! AAAGCGAGAGAATTTTCTGA+AGG + chr2_3:55437928-55437947 Msa0300100:CDS 35.0%
TGAAGGACAAAACGTAATCA+TGG - chr2_3:55438330-55438349 None:intergenic 35.0%
TTATGGTTTCTGCCAATGTT+AGG + chr2_3:55438533-55438552 Msa0300100:intron 35.0%
CCATTACATAAAGCTTATCC+AGG - chr2_3:55438583-55438602 None:intergenic 35.0%
! CCTGGATAAGCTTTATGTAA+TGG + chr2_3:55438580-55438599 Msa0300100:intron 35.0%
AACGAATATGGTTAACGCTA+TGG - chr2_3:55438685-55438704 None:intergenic 35.0%
! ACTATACACCAGCTTTTAAG+TGG - chr2_3:55438816-55438835 None:intergenic 35.0%
GAAGATAAAATGTAACAGCC+AGG - chr2_3:55438846-55438865 None:intergenic 35.0%
ATATGTGCTAAGGAATGGAT+TGG - chr2_3:55438868-55438887 None:intergenic 35.0%
!!! TGTCTTGCTTGTTTTTGAAG+AGG + chr2_3:55438911-55438930 Msa0300100:intron 35.0%
ATGCAAAGATTAACCTGTTC+TGG + chr2_3:55439008-55439027 Msa0300100:CDS 35.0%
TGCAAAGATTAACCTGTTCT+GGG + chr2_3:55439009-55439028 Msa0300100:CDS 35.0%
TCTCTAACTTCAAAACTTGC+AGG - chr2_3:55439085-55439104 None:intergenic 35.0%
!!! TGCAAGTTTTGAAGTTAGAG+AGG + chr2_3:55439084-55439103 Msa0300100:CDS 35.0%
! AACTTTGGGGGTAATGTTGA+AGG + chr2_3:55436497-55436516 Msa0300100:five_prime_UTR 40.0%
!! AAGGTTGATGATAGGCTAAG+TGG + chr2_3:55436516-55436535 Msa0300100:five_prime_UTR 40.0%
!! AGGTTGATGATAGGCTAAGT+GGG + chr2_3:55436517-55436536 Msa0300100:five_prime_UTR 40.0%
GAAATACGCGAGAATATGTG+TGG + chr2_3:55436539-55436558 Msa0300100:five_prime_UTR 40.0%
TGTGTGGAGATAGATTTGGA+TGG + chr2_3:55436555-55436574 Msa0300100:five_prime_UTR 40.0%
CTCCAATATGAAGGACTTGA+CGG + chr2_3:55436621-55436640 Msa0300100:five_prime_UTR 40.0%
TCCAATATGAAGGACTTGAC+GGG + chr2_3:55436622-55436641 Msa0300100:five_prime_UTR 40.0%
GGTCAATATGGACACAAGAA+AGG + chr2_3:55436660-55436679 Msa0300100:five_prime_UTR 40.0%
TGAAGTTTGCGAGAAAATGC+AGG + chr2_3:55436704-55436723 Msa0300100:five_prime_UTR 40.0%
GAAGTTTGCGAGAAAATGCA+GGG + chr2_3:55436705-55436724 Msa0300100:five_prime_UTR 40.0%
GGAAGTAGAGCTATGTTTGA+TGG + chr2_3:55436764-55436783 Msa0300100:five_prime_UTR 40.0%
AGTAGAGCTATGTTTGATGG+AGG + chr2_3:55436767-55436786 Msa0300100:five_prime_UTR 40.0%
TGTTTGATGGAGGACTTGAT+TGG + chr2_3:55436777-55436796 Msa0300100:five_prime_UTR 40.0%
! GATGGAGCTTTATCAAGTCA+TGG + chr2_3:55436830-55436849 Msa0300100:five_prime_UTR 40.0%
AGACAAAAGCAATTCGAGAG+CGG + chr2_3:55436918-55436937 Msa0300100:five_prime_UTR 40.0%
!! GCTGCTTCTCTTTTAACTCA+AGG - chr2_3:55436953-55436972 None:intergenic 40.0%
!! GCATTTTGAAAGCCGAATGA+AGG + chr2_3:55437064-55437083 Msa0300100:five_prime_UTR 40.0%
TCGACTCAAGTTCCTTCATT+CGG - chr2_3:55437079-55437098 None:intergenic 40.0%
TCGAAAGAGAAGCAACATGA+AGG + chr2_3:55437095-55437114 Msa0300100:five_prime_UTR 40.0%
!! GCTTTTTGAAAGCCGAATGA+AGG + chr2_3:55437202-55437221 Msa0300100:five_prime_UTR 40.0%
GCTGTTTGAAAGGCAAGTTA+AGG + chr2_3:55437327-55437346 Msa0300100:five_prime_UTR 40.0%
TGAAAGGCAAGTTAAGGAGT+TGG + chr2_3:55437333-55437352 Msa0300100:five_prime_UTR 40.0%
TCAATACCAGCTCCTTCATT+TGG - chr2_3:55437426-55437445 None:intergenic 40.0%
GCATTTCGAAAGTCGAATGA+AGG + chr2_3:55437453-55437472 Msa0300100:CDS 40.0%
AAGAGAAGCAACATGAAGGT+CGG + chr2_3:55437740-55437759 Msa0300100:CDS 40.0%
TCCGAAGAGATGCAATTGAA+AGG + chr2_3:55437862-55437881 Msa0300100:CDS 40.0%
CTGGTTTCAGATCTATCACT+CGG - chr2_3:55437954-55437973 None:intergenic 40.0%
CTCACTCAGAATTTAAACCC+TGG + chr2_3:55438421-55438440 Msa0300100:intron 40.0%
TATCCAGGAAAGCTTCAGTT+AGG - chr2_3:55438568-55438587 None:intergenic 40.0%
TTTGCACACCACTTAAAAGC+TGG + chr2_3:55438805-55438824 Msa0300100:intron 40.0%
GCAAAGATTAACCTGTTCTG+GGG + chr2_3:55439010-55439029 Msa0300100:CDS 40.0%
TCCCGTCAAGTCCTTCATAT+TGG - chr2_3:55436626-55436645 None:intergenic 45.0%
GACTTGACGGGATATGCTTT+CGG + chr2_3:55436634-55436653 Msa0300100:five_prime_UTR 45.0%
! TGCTTTCGGTGTGGTCAATA+TGG + chr2_3:55436648-55436667 Msa0300100:five_prime_UTR 45.0%
! TGTCGAGGATTTTGTAGCGA+AGG + chr2_3:55436743-55436762 Msa0300100:five_prime_UTR 45.0%
GCATTTGGAAAGCCAAGTGA+AGG + chr2_3:55436872-55436891 Msa0300100:five_prime_UTR 45.0%
GAAGGAGCTGGTGAATGAAT+TGG + chr2_3:55436890-55436909 Msa0300100:five_prime_UTR 45.0%
AAGAGAAGCAGCATGAAGGT+CGG + chr2_3:55436961-55436980 Msa0300100:five_prime_UTR 45.0%
AAGAGAAGCAACATGAAGGC+CGG + chr2_3:55437099-55437118 Msa0300100:five_prime_UTR 45.0%
TCGACTCAAGGTCCTTCATT+CGG - chr2_3:55437217-55437236 None:intergenic 45.0%
TGAATGTCAAGTGAAGGAGC+AGG + chr2_3:55437291-55437310 Msa0300100:five_prime_UTR 45.0%
! CCTTCATCCGGTCTTCATTT+TGG - chr2_3:55437498-55437517 None:intergenic 45.0%
CCAAAATGAAGACCGGATGA+AGG + chr2_3:55437495-55437514 Msa0300100:CDS 45.0%
TTGAGTCATGCTCCTTCATC+CGG - chr2_3:55437510-55437529 None:intergenic 45.0%
! CCTTTGTTTGGCCTTTGAGT+TGG - chr2_3:55437624-55437643 None:intergenic 45.0%
CCAACTCAAAGGCCAAACAA+AGG + chr2_3:55437621-55437640 Msa0300100:CDS 45.0%
AGAGAAGCAACATGAAGGTC+GGG + chr2_3:55437741-55437760 Msa0300100:CDS 45.0%
TGAAAGTAAAGTGGAGGAGC+TGG + chr2_3:55437795-55437814 Msa0300100:CDS 45.0%
!! GATGCATTTTGAAAGCCAGG+TGG + chr2_3:55437828-55437847 Msa0300100:CDS 45.0%
! CGAGTGATAGATCTGAAACC+AGG + chr2_3:55437952-55437971 Msa0300100:CDS 45.0%
! GATCTGAAACCAGGAACTGA+TGG + chr2_3:55437961-55437980 Msa0300100:CDS 45.0%
! GAAGGTTGGTTGCTGTTCAT+CGG + chr2_3:55437999-55438018 Msa0300100:CDS 45.0%
CGAGCTGAAGCTATCAAAGA+CGG - chr2_3:55438054-55438073 None:intergenic 45.0%
TTCCAGTTTCGTCTCCAACT+AGG - chr2_3:55438098-55438117 None:intergenic 45.0%
GTTGCGAGCTGTGAAGATTA+TGG - chr2_3:55438121-55438140 None:intergenic 45.0%
CTGCATTCAAGTAGGATGTG+AGG + chr2_3:55438485-55438504 Msa0300100:intron 45.0%
CAAGTAGGATGTGAGGAACT+AGG + chr2_3:55438492-55438511 Msa0300100:intron 45.0%
AGGACACTGAGTCCTAACAT+TGG - chr2_3:55438548-55438567 None:intergenic 45.0%
TGTCCTAACTGAAGCTTTCC+TGG + chr2_3:55438562-55438581 Msa0300100:intron 45.0%
CCTGCAGCTGGGATATATAT+GGG - chr2_3:55438647-55438666 None:intergenic 45.0%
CCATATATATCCCAGCTGCA+GGG + chr2_3:55438645-55438664 Msa0300100:intron 45.0%
TGGTTAACGCTATGGATGCA+TGG - chr2_3:55438677-55438696 None:intergenic 45.0%
TGGTGTATAGTAAGTTGCCC+TGG + chr2_3:55438825-55438844 Msa0300100:intron 45.0%
! AGGACTTGATTGGAGAGTGC+AGG + chr2_3:55436787-55436806 Msa0300100:five_prime_UTR 50.0%
CATTCACCAGCTCCTTCACT+TGG - chr2_3:55436887-55436906 None:intergenic 50.0%
GCAATTCGAGAGCGGAATGA+AGG + chr2_3:55436926-55436945 Msa0300100:five_prime_UTR 50.0%
AGAGAAGCAGCATGAAGGTC+GGG + chr2_3:55436962-55436981 Msa0300100:five_prime_UTR 50.0%
AGAGAAGCAACATGAAGGCC+GGG + chr2_3:55437100-55437119 Msa0300100:five_prime_UTR 50.0%
TCGATTCATGCTCCTCTACC+CGG - chr2_3:55437121-55437140 None:intergenic 50.0%
TGCAATATGAAGCCGGGTGA+AGG + chr2_3:55437243-55437262 Msa0300100:five_prime_UTR 50.0%
TTGATCTGTGCTCCTTCACC+CGG - chr2_3:55437258-55437277 None:intergenic 50.0%
CGAAAGCCAAATGAAGGAGC+TGG + chr2_3:55437417-55437436 Msa0300100:CDS 50.0%
!! GCATTTTGAAAGCCAGGTGG+AGG + chr2_3:55437831-55437850 Msa0300100:CDS 50.0%
AGAGTGTGTCCATCAGTTCC+TGG - chr2_3:55437973-55437992 None:intergenic 50.0%
TGGACACACTCTGACAGTGA+AGG + chr2_3:55437981-55438000 Msa0300100:CDS 50.0%
CACACTCTGACAGTGAAGGT+TGG + chr2_3:55437985-55438004 Msa0300100:CDS 50.0%
TCAAAGACGGCCATTCATCG+CGG - chr2_3:55438041-55438060 None:intergenic 50.0%
AACTAGGCATTCAGCGATGC+GGG - chr2_3:55438082-55438101 None:intergenic 50.0%
CAACTAGGCATTCAGCGATG+CGG - chr2_3:55438083-55438102 None:intergenic 50.0%
TGCCTAGTTGGAGACGAAAC+TGG + chr2_3:55438093-55438112 Msa0300100:CDS 50.0%
TTCACAGCTCGCAACGAACA+GGG + chr2_3:55438126-55438145 Msa0300100:CDS 50.0%
CCCATATATATCCCAGCTGC+AGG + chr2_3:55438644-55438663 Msa0300100:intron 50.0%
CCCTGCAGCTGGGATATATA+TGG - chr2_3:55438648-55438667 None:intergenic 50.0%
CAGTAGCACCAGGTTTCATG+AGG - chr2_3:55438976-55438995 None:intergenic 50.0%
CGCAGAATTACAGTAGCACC+AGG - chr2_3:55438986-55439005 None:intergenic 50.0%
!! ACCTGTTCTGGGGACATATG+AGG + chr2_3:55439020-55439039 Msa0300100:CDS 50.0%
CTGTGAGCTGTATACATCCC+CGG - chr2_3:55439060-55439079 None:intergenic 50.0%
GACGGGATATGCTTTCGGTG+TGG + chr2_3:55436639-55436658 Msa0300100:five_prime_UTR 55.0%
! CTTGATTGGAGAGTGCAGGC+AGG + chr2_3:55436791-55436810 Msa0300100:five_prime_UTR 55.0%
GGAAAGCCAAGTGAAGGAGC+TGG + chr2_3:55436878-55436897 Msa0300100:five_prime_UTR 55.0%
GCAACATGAAGGTCGGGTGA+AGG + chr2_3:55437747-55437766 Msa0300100:CDS 55.0%
CGCATCGCTGAATGCCTAGT+TGG + chr2_3:55438081-55438100 Msa0300100:CDS 55.0%
CTTCACAGCTCGCAACGAAC+AGG + chr2_3:55438125-55438144 Msa0300100:CDS 55.0%
ATGGTTGCAACCCTGCAGCT+GGG - chr2_3:55438658-55438677 None:intergenic 55.0%
GCAGTGGACCTCATGAAACC+TGG + chr2_3:55438965-55438984 Msa0300100:intron 55.0%
GCCTCATATGTCCCCAGAAC+AGG - chr2_3:55439024-55439043 None:intergenic 55.0%
!! GTTCTGGGGACATATGAGGC+TGG + chr2_3:55439024-55439043 Msa0300100:CDS 55.0%
TGAGGCTGGCAGTTGATTGC+CGG + chr2_3:55439038-55439057 Msa0300100:CDS 55.0%
GCAGCATGAAGGTCGGGTGA+AGG + chr2_3:55436968-55436987 Msa0300100:five_prime_UTR 60.0%
GCAACATGAAGGCCGGGTAG+AGG + chr2_3:55437106-55437125 Msa0300100:five_prime_UTR 60.0%
CGGATTTGAGCTCCTCCACC+TGG - chr2_3:55437846-55437865 None:intergenic 60.0%
AGGCATTCAGCGATGCGGGA+GGG - chr2_3:55438078-55438097 None:intergenic 60.0%
TAGGCATTCAGCGATGCGGG+AGG - chr2_3:55438079-55438098 None:intergenic 60.0%
CATGGTTGCAACCCTGCAGC+TGG - chr2_3:55438659-55438678 None:intergenic 60.0%
GAGGCTGGCAGTTGATTGCC+GGG + chr2_3:55439039-55439058 Msa0300100:CDS 60.0%
AGGCTGGCAGTTGATTGCCG+GGG + chr2_3:55439040-55439059 Msa0300100:CDS 60.0%
TTGCCCCCTGCCGCGATGAA+TGG + chr2_3:55438028-55438047 Msa0300100:CDS 65.0%
ACGGCCATTCATCGCGGCAG+GGG - chr2_3:55438035-55438054 None:intergenic 65.0%
GACGGCCATTCATCGCGGCA+GGG - chr2_3:55438036-55438055 None:intergenic 65.0%
AGACGGCCATTCATCGCGGC+AGG - chr2_3:55438037-55438056 None:intergenic 65.0%
GGCATTCAGCGATGCGGGAG+GGG - chr2_3:55438077-55438096 None:intergenic 65.0%
CGGCCATTCATCGCGGCAGG+GGG - chr2_3:55438034-55438053 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 55436494 55439159 55436494 ID=Msa0300100;Name=Msa0300100
chr2_3 mRNA 55436494 55439159 55436494 ID=Msa0300100-mRNA-1;Parent=Msa0300100;Name=Msa0300100-mRNA-1;_AED=0.22;_eAED=0.22;_QI=891|1|0.5|1|1|1|2|0|317
chr2_3 exon 55436494 55438147 55436494 ID=Msa0300100-mRNA-1:exon:17720;Parent=Msa0300100-mRNA-1
chr2_3 exon 55438969 55439159 55438969 ID=Msa0300100-mRNA-1:exon:17721;Parent=Msa0300100-mRNA-1
chr2_3 five_prime_UTR 55436494 55437384 55436494 ID=Msa0300100-mRNA-1:five_prime_utr;Parent=Msa0300100-mRNA-1
chr2_3 CDS 55437385 55438147 55437385 ID=Msa0300100-mRNA-1:cds;Parent=Msa0300100-mRNA-1
chr2_3 CDS 55438969 55439159 55438969 ID=Msa0300100-mRNA-1:cds;Parent=Msa0300100-mRNA-1
Gene Sequence

>Msa0300100

ATGAAGGAGTTTGAGTCAATAGAGAGAGAATTCGAAAGCCAAATGAAGGAGCTGGTATTGAAACAGAAGCATTTCGAAAGTCGAATGAAGGAACTTGAGTTGAAAGAGAACCAAAATGAAGACCGGATGAAGGAGCATGACTCAAAGAAGCGAGAATTTGAAGGTCAAGTGAAGGAGCTAGAATCTAAGAAGAAGCACTTTGACAGCCAAGTCAAACTGTTTGAGTCAAATTATAACCAACTCAAAGGCCAAACAAAGGAGTTCTTTTTAAAAGAGAGGGAATTTGAAAGCAAAATGAAGAAACTGGTAGTGAAAAAGAGACGTTTTGAAGGCCAGATGAAGATGTTTGAGTTGAAAGAGAAGCAACATGAAGGTCGGGTGAAGGAGCATGAATCAAGAGCTAGAGAATTTGAAAGTAAAGTGGAGGAGCTGGAATGTAAAAAGATGCATTTTGAAAGCCAGGTGGAGGAGCTCAAATCCGAAGAGATGCAATTGAAAGGACAAATTAAAGAATTTGAAGGCCAAATGAAGGAGTTTAAATCAAAAGCGAGAGAATTTTCTGAAGGCCGAGTGATAGATCTGAAACCAGGAACTGATGGACACACTCTGACAGTGAAGGTTGGTTGCTGTTCATCGGTGAAGATTGCCCCCTGCCGCGATGAATGGCCGTCTTTGATAGCTTCAGCTCGCCCCTCCCGCATCGCTGAATGCCTAGTTGGAGACGAAACTGGAACCATAATCTTCACAGCTCGCAACGAACAGGTGGACCTCATGAAACCTGGTGCTACTGTAATTCTGCGTAATGCAAAGATTAACCTGTTCTGGGGACATATGAGGCTGGCAGTTGATTGCCGGGGATGTATACAGCTCACAGAACCTGCAAGTTTTGAAGTTAGAGAGGATAACAATCTTTCACTAGATAAATATGATGAATTTAGTAGCCGATGTCGTTGA
Protein sequence

>Msa0300100

MKEFESIEREFESQMKELVLKQKHFESRMKELELKENQNEDRMKEHDSKKREFEGQVKELESKKKHFDSQVKLFESNYNQLKGQTKEFFLKEREFESKMKKLVVKKRRFEGQMKMFELKEKQHEGRVKEHESRAREFESKVEELECKKMHFESQVEELKSEEMQLKGQIKEFEGQMKEFKSKAREFSEGRVIDLKPGTDGHTLTVKVGCCSSVKIAPCRDEWPSLIASARPSRIAECLVGDETGTIIFTARNEQVDLMKPGATVILRNAKINLFWGHMRLAVDCRGCIQLTEPASFEVREDNNLSLDKYDEFSSRCR*