AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300450 MtrunA17_Chr2g0325501 98.258 287 4 1 1 286 1 287 0.0 578
Msa0300450 MtrunA17_Chr1g0183691 35.600 250 142 5 18 259 5 243 1.99e-41 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300450 AT3G58600.1 66.787 277 83 4 18 286 27 302 2.15e-114 332
Msa0300450 AT1G03900.1 44.311 167 84 3 23 183 12 175 4.38e-39 138

Find 102 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGAAATCTTTCTCTGTTTC+CGG 0.158594 2_3:-55906811 Msa0300450:five_prime_UTR
GTCTTCTGAAGATATGAATT+TGG 0.165545 2_3:+55906877 None:intergenic
AAACCCGACATTCAGAAAAT+TGG 0.187508 2_3:-55906767 Msa0300450:CDS
AAGCTGCACCGAAGCGATTC+TGG 0.249439 2_3:+55906972 None:intergenic
GTCCATATCTGTTTGTTAAT+TGG 0.252680 2_3:-55903606 Msa0300450:three_prime_UTR
GATTAGGGTTTGGTAGATTT+TGG 0.253778 2_3:+55906935 None:intergenic
GAATGAGTGCAGCTTCTTAC+AGG 0.257166 2_3:-55906401 Msa0300450:intron
TCTTCTGAAGATATGAATTT+GGG 0.287985 2_3:+55906878 None:intergenic
CCACAATCATTCATAATAGA+TGG 0.298169 2_3:+55903670 None:intergenic
CCGTCTGATTTGAACACCTT+TGG 0.302053 2_3:+55907011 None:intergenic
CTTTCTCTGTTTCCGGATCA+TGG 0.307728 2_3:-55906804 Msa0300450:exon
GAGAAAGATTTCGAATTTGA+TGG 0.310318 2_3:+55906820 None:intergenic
GACAGTGAGGAATCGAATTT+CGG 0.321190 2_3:+55906911 None:intergenic
TAAGAAGCTGCACTCATTCT+TGG 0.323176 2_3:+55906405 None:intergenic
GAATCGAATTTCGGAGATTA+GGG 0.329332 2_3:+55906920 None:intergenic
GGAATCGAATTTCGGAGATT+AGG 0.349872 2_3:+55906919 None:intergenic
CATAAGCCTCTGTTCTTTCT+CGG 0.358789 2_3:+55904362 None:intergenic
CGTCTGATTTGAACACCTTT+GGG 0.362526 2_3:+55907012 None:intergenic
AATCAACTGAGGAGCTTTGC+TGG 0.368512 2_3:+55904147 None:intergenic
GGAGACTTCTGCGGGGTAAC+AGG 0.373330 2_3:+55903854 None:intergenic
TGAAGGTTCGGTTCATAGAT+TGG 0.375896 2_3:+55907052 None:intergenic
GATGTAGTAGATGATGATTT+TGG 0.381931 2_3:-55903722 Msa0300450:CDS
TTGGTGGTTATCTTAGTTGC+GGG 0.385750 2_3:-55903583 Msa0300450:three_prime_UTR
AACCAATTAACAAACAGATA+TGG 0.402496 2_3:+55903604 None:intergenic
AAGTACATAACCACTGAATC+TGG 0.406019 2_3:+55903407 None:intergenic
GGCGCATCTCAATAGTCATT+TGG 0.409262 2_3:-55903487 Msa0300450:three_prime_UTR
TTCATTTCAGGGCTGATGAA+TGG 0.412505 2_3:-55905920 Msa0300450:intron
AATGAAGAACAAAAGCGATC+TGG 0.425569 2_3:+55906854 None:intergenic
TTGTGGATACCTTGTTCATC+TGG 0.437607 2_3:-55903653 Msa0300450:three_prime_UTR
GAAGGTTCGGTTCATAGATT+GGG 0.439722 2_3:+55907053 None:intergenic
TAGTCATTTGGTGCTGATCA+AGG 0.444839 2_3:-55903475 Msa0300450:three_prime_UTR
ACGGTCTGAAATTGAAGGTT+CGG 0.449625 2_3:+55907040 None:intergenic
TCATTTCAGGGCTGATGAAT+GGG 0.449823 2_3:-55905919 Msa0300450:intron
AACCCGACATTCAGAAAATT+GGG 0.462440 2_3:-55906766 Msa0300450:CDS
TTGATTACAGTTTGAAAGAA+GGG 0.463667 2_3:-55904130 Msa0300450:CDS
GATATTGAAAGTTGTTAGCA+AGG 0.466773 2_3:-55905871 Msa0300450:CDS
ATGAATGGGATGTCAACAAA+TGG 0.473047 2_3:-55905905 Msa0300450:CDS
GTTGATTACAGTTTGAAAGA+AGG 0.478319 2_3:-55904131 Msa0300450:CDS
ATATCTGTTTGTTAATTGGT+TGG 0.480576 2_3:-55903602 Msa0300450:three_prime_UTR
AGTGAGTGGAGTGATTGCTT+TGG 0.481595 2_3:+55907078 None:intergenic
TAACAGGTGAACCTGGTGAT+GGG 0.483068 2_3:+55903870 None:intergenic
TATCCTCCTTCTCCATGATC+CGG 0.493809 2_3:+55906792 None:intergenic
ATGAAGAACAAAAGCGATCT+GGG 0.497981 2_3:+55906855 None:intergenic
GTTGGTGGTTATCTTAGTTG+CGG 0.506423 2_3:-55903584 Msa0300450:three_prime_UTR
ATAACAGCTTCCACGGGATG+TGG 0.507930 2_3:+55905683 None:intergenic
AGTCTGTTGGAGACTTCTGC+GGG 0.508559 2_3:+55903846 None:intergenic
GAATTTCGGAGATTAGGGTT+TGG 0.508940 2_3:+55906925 None:intergenic
GATTTCGAATTTGATGGAAG+GGG 0.511303 2_3:+55906826 None:intergenic
TCTGTTTGTTAATTGGTTGG+TGG 0.513135 2_3:-55903599 Msa0300450:three_prime_UTR
AAGCTGTTATTGACAGCAGC+AGG 0.513305 2_3:-55905670 Msa0300450:intron
AGATTTCGAATTTGATGGAA+GGG 0.516307 2_3:+55906825 None:intergenic
GTCAACAAATGGGCATGGGA+AGG 0.517214 2_3:-55905894 Msa0300450:CDS
ATCTGACGGTCTGAAATTGA+AGG 0.520220 2_3:+55907035 None:intergenic
AAAGAAAACTGCAGAAGAGA+TGG 0.526513 2_3:-55904180 Msa0300450:CDS
TGCGGGGTAACAGGTGAACC+TGG 0.526812 2_3:+55903863 None:intergenic
AAGATTTCGAATTTGATGGA+AGG 0.527298 2_3:+55906824 None:intergenic
TATGATCGTGCAAGGCAGCT+TGG 0.527856 2_3:+55904335 None:intergenic
AGTCATTTGGTGCTGATCAA+GGG 0.533892 2_3:-55903474 Msa0300450:three_prime_UTR
GTAACAGGTGAACCTGGTGA+TGG 0.537068 2_3:+55903869 None:intergenic
CCATCTATTATGAATGATTG+TGG 0.537988 2_3:-55903670 Msa0300450:three_prime_UTR
CACAATCATTCATAATAGAT+GGG 0.543332 2_3:+55903671 None:intergenic
AACAGGTGAACCTGGTGATG+GGG 0.546112 2_3:+55903871 None:intergenic
TGAACACCTTTGGGATCTGA+CGG 0.548857 2_3:+55907021 None:intergenic
TCATAGATTGGGACAGTGAG+TGG 0.555654 2_3:+55907064 None:intergenic
GAAGCTGCACTCATTCTTGG+AGG 0.557836 2_3:+55906408 None:intergenic
CTGTTTCCGGATCATGGAGA+AGG 0.558980 2_3:-55906798 Msa0300450:exon
GAGTCTGTTGGAGACTTCTG+CGG 0.568129 2_3:+55903845 None:intergenic
ATATTGAAAGTTGTTAGCAA+GGG 0.568944 2_3:-55905870 Msa0300450:CDS
TCAACAAATGGGCATGGGAA+GGG 0.569352 2_3:-55905893 Msa0300450:CDS
TGATTACAGTTTGAAAGAAG+GGG 0.573861 2_3:-55904129 Msa0300450:CDS
TACTATGTAGACGTAACACT+CGG 0.574408 2_3:+55906694 None:intergenic
GGGGAAGAAATTTGACAGTG+AGG 0.575596 2_3:+55906898 None:intergenic
GGGATGTCAACAAATGGGCA+TGG 0.576412 2_3:-55905899 Msa0300450:CDS
GCTGTCAATAACAGCTTCCA+CGG 0.579429 2_3:+55905676 None:intergenic
GTCATTTGGTGCTGATCAAG+GGG 0.594704 2_3:-55903473 Msa0300450:three_prime_UTR
CTGTCAATAACAGCTTCCAC+GGG 0.598765 2_3:+55905677 None:intergenic
GCTCAATGAACCAGATTCAG+TGG 0.598785 2_3:-55903417 Msa0300450:three_prime_UTR
GGATGTCAACAAATGGGCAT+GGG 0.603400 2_3:-55905898 Msa0300450:CDS
TGCCAATAAAAGCATGTCGA+AGG 0.603687 2_3:+55904392 None:intergenic
TGAATGGGATGTCAACAAAT+GGG 0.604897 2_3:-55905904 Msa0300450:CDS
AAATGGAGAGCCACATCCCG+TGG 0.605045 2_3:-55905693 Msa0300450:CDS
CTTCGCATAGAAGAAAACAT+AGG 0.607237 2_3:-55905549 Msa0300450:intron
ACAGGTGAACCTGGTGATGG+GGG 0.607450 2_3:+55903872 None:intergenic
CCAAAGGTGTTCAAATCAGA+CGG 0.609487 2_3:-55907011 Msa0300450:five_prime_UTR
GGTGGTGGTAACTTAATACA+TGG 0.613088 2_3:+55903896 None:intergenic
TATTGTATGCAGAGAAGCGG+CGG 0.616671 2_3:-55903995 Msa0300450:intron
ATTTATTGTATGCAGAGAAG+CGG 0.617754 2_3:-55903998 Msa0300450:intron
TTGCGGGCTAGTAGCGTGAG+AGG 0.618533 2_3:-55903567 Msa0300450:three_prime_UTR
TTTCAAACTGTAATCAACTG+AGG 0.625498 2_3:+55904136 None:intergenic
AATAAAGTGACTCAAGAGAG+AGG 0.633889 2_3:+55903538 None:intergenic
GCAGGGTGCAAACAACTCCT+CGG 0.636828 2_3:-55903948 Msa0300450:CDS
ATACTTCATATGATCGTGCA+AGG 0.645577 2_3:+55904327 None:intergenic
TTTCCGGATCATGGAGAAGG+AGG 0.645908 2_3:-55906795 Msa0300450:exon
TCCTGAAAATCAAAGCACAC+AGG 0.648603 2_3:-55903744 Msa0300450:CDS
ACTATGTAGACGTAACACTC+GGG 0.657792 2_3:+55906695 None:intergenic
AACTGAACACCAGATGAACA+AGG 0.660378 2_3:+55903644 None:intergenic
CTTCTGAAGATATGAATTTG+GGG 0.667262 2_3:+55906879 None:intergenic
AAACTAGAAGATAAGAACAC+AGG 0.671758 2_3:-55905831 Msa0300450:intron
TTCAGACCGTCAGATCCCAA+AGG 0.692418 2_3:-55907027 Msa0300450:five_prime_UTR
GTCTGTTGGAGACTTCTGCG+GGG 0.741687 2_3:+55903847 None:intergenic
AAGGTGTTCAAATCAGACGG+TGG 0.746634 2_3:-55907008 Msa0300450:five_prime_UTR
AGGATTCCGAGAAAGAACAG+AGG 0.756772 2_3:-55904368 Msa0300450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGAATTTGATTTTTTTTT+TGG - chr2_3:55904055-55904074 Msa0300450:intron 10.0%
!!! CGATTTTTTTTTTTAAATAT+CGG + chr2_3:55905650-55905669 None:intergenic 10.0%
!! CGATATTTAAAAAAAAAAAT+CGG - chr2_3:55905648-55905667 Msa0300450:intron 10.0%
!! GATATTTAAAAAAAAAAATC+GGG - chr2_3:55905649-55905668 Msa0300450:intron 10.0%
!! ATATTTAAAAAAAAAAATCG+GGG - chr2_3:55905650-55905669 Msa0300450:intron 10.0%
!! TCCAAAATTAAAAAAAAAAT+CGG - chr2_3:55905706-55905725 Msa0300450:CDS 10.0%
!! CAAATATTACTATTTAATAT+GGG + chr2_3:55906037-55906056 None:intergenic 10.0%
!! ACAAATATTACTATTTAATA+TGG + chr2_3:55906038-55906057 None:intergenic 10.0%
!!! TTTCTTTGTTTTGTTATTTT+AGG - chr2_3:55903396-55903415 Msa0300450:three_prime_UTR 15.0%
!!! CAAGATTAAATTTTCATTTT+GGG + chr2_3:55903972-55903991 None:intergenic 15.0%
!!! TAAATGAAGTATTTTTAACT+AGG - chr2_3:55904780-55904799 Msa0300450:intron 15.0%
!! CCAAAATTAAAAAAAAAATC+GGG - chr2_3:55905707-55905726 Msa0300450:CDS 15.0%
!! CAAAATTAAAAAAAAAATCG+GGG - chr2_3:55905708-55905727 Msa0300450:CDS 15.0%
!! TTCTTATTAATTATGAAGTT+TGG - chr2_3:55906076-55906095 Msa0300450:intron 15.0%
!!! TTCTTTTTATTTTTTCCAAA+CGG - chr2_3:55906278-55906297 Msa0300450:intron 15.0%
!!! TTTGTTTTGTTATTTTAGGT+AGG - chr2_3:55903400-55903419 Msa0300450:three_prime_UTR 20.0%
!!! TTGTTTTGTTATTTTAGGTA+GGG - chr2_3:55903401-55903420 Msa0300450:three_prime_UTR 20.0%
!! AATAGTATGTGATAGAAAAA+TGG - chr2_3:55903901-55903920 Msa0300450:CDS 20.0%
!! TTGATTAATTCAATTGACAA+TGG - chr2_3:55903941-55903960 Msa0300450:CDS 20.0%
!!! GCAAGATTAAATTTTCATTT+TGG + chr2_3:55903973-55903992 None:intergenic 20.0%
!! ATTTAATGTTAGTGATTAGA+TGG - chr2_3:55904030-55904049 Msa0300450:intron 20.0%
!! TTTAATGTTAGTGATTAGAT+GGG - chr2_3:55904031-55904050 Msa0300450:intron 20.0%
!! TAAAGAAGTGGTTATAAAAT+AGG + chr2_3:55904333-55904352 None:intergenic 20.0%
!!! TAGTAGTTATTTTAAAGAAG+TGG + chr2_3:55904345-55904364 None:intergenic 20.0%
!! AAAATGCATTAAAAGTAAAG+AGG + chr2_3:55904425-55904444 None:intergenic 20.0%
!! AAAAAAACAAGTTCATAAAC+TGG + chr2_3:55904528-55904547 None:intergenic 20.0%
!!! TATTATTCCTTTTTGTACTT+AGG - chr2_3:55904910-55904929 Msa0300450:intron 20.0%
!! TAATTTAAGAAAAATGCTCA+CGG + chr2_3:55905156-55905175 None:intergenic 20.0%
!!! ATTCCATTATATTTTGTTCA+AGG + chr2_3:55905191-55905210 None:intergenic 20.0%
!!! AAAATATAATGGAATTTAGC+CGG - chr2_3:55905196-55905215 Msa0300450:intron 20.0%
!! TAAACATGAACAAAAATTTC+AGG - chr2_3:55905476-55905495 Msa0300450:intron 20.0%
!!! CCCGATTTTTTTTTTAATTT+TGG + chr2_3:55905710-55905729 None:intergenic 20.0%
!!! TGTTTCTTTAACTATTTTAG+AGG + chr2_3:55906745-55906764 None:intergenic 20.0%
!!! TGTTTTGTTATTTTAGGTAG+GGG - chr2_3:55903402-55903421 Msa0300450:three_prime_UTR 25.0%
!! TCTTCTGAAGATATGAATTT+GGG + chr2_3:55903648-55903667 None:intergenic 25.0%
!! TTAGGTGCACAATAATTTTT+GGG + chr2_3:55903995-55904014 None:intergenic 25.0%
!! TTTAGGTGCACAATAATTTT+TGG + chr2_3:55903996-55904015 None:intergenic 25.0%
! TTAATGTTAGTGATTAGATG+GGG - chr2_3:55904032-55904051 Msa0300450:intron 25.0%
! GATGTTATTAATGTTCATGA+TGG - chr2_3:55904205-55904224 Msa0300450:intron 25.0%
!! TTTTAATGAATATGCGTTGA+CGG - chr2_3:55904252-55904271 Msa0300450:intron 25.0%
! ACTAAATGCTCAATTCTTAT+TGG - chr2_3:55904361-55904380 Msa0300450:CDS 25.0%
! ATATATGTGAAGTTCATTTC+AGG - chr2_3:55904591-55904610 Msa0300450:intron 25.0%
! TATATGTGAAGTTCATTTCA+GGG - chr2_3:55904592-55904611 Msa0300450:intron 25.0%
! ATATTGAAAGTTGTTAGCAA+GGG - chr2_3:55904653-55904672 Msa0300450:intron 25.0%
! AATGCATTGAGTGTTTATTA+TGG - chr2_3:55905235-55905254 Msa0300450:intron 25.0%
! TCAGATATATGATTGAAAGT+GGG + chr2_3:55905302-55905321 None:intergenic 25.0%
! ATCAGATATATGATTGAAAG+TGG + chr2_3:55905303-55905322 None:intergenic 25.0%
! ATGAGAACAAAAATACTAGA+AGG - chr2_3:55905376-55905395 Msa0300450:intron 25.0%
!! TATTTGTTTGAGATCGATAT+AGG - chr2_3:55905417-55905436 Msa0300450:intron 25.0%
! ATGGAACCAAATATCATATT+AGG - chr2_3:55905861-55905880 Msa0300450:CDS 25.0%
! TGGAACCAAATATCATATTA+GGG - chr2_3:55905862-55905881 Msa0300450:CDS 25.0%
! GATTACCCTAATATGATATT+TGG + chr2_3:55905870-55905889 None:intergenic 25.0%
! TAAATCCAAAATATGCTAGA+TGG + chr2_3:55906226-55906245 None:intergenic 25.0%
! AGGTATCTTCAAAACAATTT+AGG + chr2_3:55906317-55906336 None:intergenic 25.0%
!!! TAAATTGTTTTGAAGATACC+TGG - chr2_3:55906316-55906335 Msa0300450:intron 25.0%
! TTGATTACAGTTTGAAAGAA+GGG - chr2_3:55906393-55906412 Msa0300450:intron 25.0%
! CACAATCATTCATAATAGAT+GGG + chr2_3:55906855-55906874 None:intergenic 25.0%
! AACCAATTAACAAACAGATA+TGG + chr2_3:55906922-55906941 None:intergenic 25.0%
!! ATATCTGTTTGTTAATTGGT+TGG - chr2_3:55906921-55906940 Msa0300450:five_prime_UTR 25.0%
!!! TATGCTTTGTTTTCATTTCT+TGG - chr2_3:55907015-55907034 Msa0300450:five_prime_UTR 25.0%
! CTTCTGAAGATATGAATTTG+GGG + chr2_3:55903647-55903666 None:intergenic 30.0%
! GTCTTCTGAAGATATGAATT+TGG + chr2_3:55903649-55903668 None:intergenic 30.0%
AGATTTCGAATTTGATGGAA+GGG + chr2_3:55903701-55903720 None:intergenic 30.0%
AAGATTTCGAATTTGATGGA+AGG + chr2_3:55903702-55903721 None:intergenic 30.0%
GAGAAAGATTTCGAATTTGA+TGG + chr2_3:55903706-55903725 None:intergenic 30.0%
TGAAAAAGAACGAGTTCTAT+GGG + chr2_3:55903804-55903823 None:intergenic 30.0%
TTGAAAAAGAACGAGTTCTA+TGG + chr2_3:55903805-55903824 None:intergenic 30.0%
!!! TTTTTTTGGTGTCAAGCTAA+TGG - chr2_3:55904069-55904088 Msa0300450:intron 30.0%
!!! TTTTTTGGTGTCAAGCTAAT+GGG - chr2_3:55904070-55904089 Msa0300450:intron 30.0%
TTGTTTGTTCATCTTAAGCT+TGG - chr2_3:55904178-55904197 Msa0300450:CDS 30.0%
AATAGGCATACATAGCAATA+TGG + chr2_3:55904316-55904335 None:intergenic 30.0%
GATATTGAAAGTTGTTAGCA+AGG - chr2_3:55904652-55904671 Msa0300450:intron 30.0%
AAACTAGAAGATAAGAACAC+AGG - chr2_3:55904692-55904711 Msa0300450:intron 30.0%
!! AGGTTATTTTCCATAACTGT+TGG - chr2_3:55904873-55904892 Msa0300450:intron 30.0%
CTAAAATCTACCAACAGTTA+TGG + chr2_3:55904886-55904905 None:intergenic 30.0%
TAAGCATCCTAAGTACAAAA+AGG + chr2_3:55904920-55904939 None:intergenic 30.0%
TAAAACAACTACAACCTGTT+TGG - chr2_3:55905104-55905123 Msa0300450:intron 30.0%
!!! TTTTTGGAACTAAACCAAAC+AGG + chr2_3:55905121-55905140 None:intergenic 30.0%
! CCATTATATTTTGTTCAAGG+TGG + chr2_3:55905188-55905207 None:intergenic 30.0%
CCACCTTGAACAAAATATAA+TGG - chr2_3:55905185-55905204 Msa0300450:intron 30.0%
! GTGTTAGTTTCGAAAATTCA+AGG + chr2_3:55905353-55905372 None:intergenic 30.0%
AAAATAGCAATGAACACTGA+TGG - chr2_3:55905612-55905631 Msa0300450:intron 30.0%
AAAAAAAAAATCGGGGAAAG+AGG - chr2_3:55905657-55905676 Msa0300450:intron 30.0%
AAAAAAAAAATCGGGGAAAG+AGG - chr2_3:55905657-55905676 Msa0300450:intron 30.0%
! CAGTTGCAAAATGGTATTTT+GGG + chr2_3:55905771-55905790 None:intergenic 30.0%
! ACAGTTGCAAAATGGTATTT+TGG + chr2_3:55905772-55905791 None:intergenic 30.0%
CATATTAGGGTAATCTACAT+TGG - chr2_3:55905875-55905894 Msa0300450:CDS 30.0%
AACTTCCTCATGCTAAAAAT+AGG + chr2_3:55905977-55905996 None:intergenic 30.0%
!!! ATTTTTAGCATGAGGAAGTT+TGG - chr2_3:55905977-55905996 Msa0300450:intron 30.0%
!! GAAGTTTGGACATTTTAAGT+TGG - chr2_3:55905991-55906010 Msa0300450:intron 30.0%
TTTCAAACTGTAATCAACTG+AGG + chr2_3:55906390-55906409 None:intergenic 30.0%
GTTGATTACAGTTTGAAAGA+AGG - chr2_3:55906392-55906411 Msa0300450:intron 30.0%
TGATTACAGTTTGAAAGAAG+GGG - chr2_3:55906394-55906413 Msa0300450:intron 30.0%
CTGCATACAATAAATAGGAT+AGG + chr2_3:55906520-55906539 None:intergenic 30.0%
CTTCTCTGCATACAATAAAT+AGG + chr2_3:55906525-55906544 None:intergenic 30.0%
ATTTATTGTATGCAGAGAAG+CGG - chr2_3:55906525-55906544 Msa0300450:intron 30.0%
! TTCTCAAGGCTTAATTTTGT+AGG + chr2_3:55906705-55906724 None:intergenic 30.0%
! GATGTAGTAGATGATGATTT+TGG - chr2_3:55906801-55906820 Msa0300450:exon 30.0%
CCACAATCATTCATAATAGA+TGG + chr2_3:55906856-55906875 None:intergenic 30.0%
CCATCTATTATGAATGATTG+TGG - chr2_3:55906853-55906872 Msa0300450:five_prime_UTR 30.0%
! GTCCATATCTGTTTGTTAAT+TGG - chr2_3:55906917-55906936 Msa0300450:five_prime_UTR 30.0%
!! GATTAGGGTTTGGTAGATTT+TGG + chr2_3:55903591-55903610 None:intergenic 35.0%
GAATCGAATTTCGGAGATTA+GGG + chr2_3:55903606-55903625 None:intergenic 35.0%
ATGAAGAACAAAAGCGATCT+GGG + chr2_3:55903671-55903690 None:intergenic 35.0%
AATGAAGAACAAAAGCGATC+TGG + chr2_3:55903672-55903691 None:intergenic 35.0%
GATTTCGAATTTGATGGAAG+GGG + chr2_3:55903700-55903719 None:intergenic 35.0%
TCGAAATCTTTCTCTGTTTC+CGG - chr2_3:55903712-55903731 Msa0300450:CDS 35.0%
AAACCCGACATTCAGAAAAT+TGG - chr2_3:55903756-55903775 Msa0300450:CDS 35.0%
AACCCGACATTCAGAAAATT+GGG - chr2_3:55903757-55903776 Msa0300450:CDS 35.0%
! TTCCCAATTTTCTGAATGTC+GGG + chr2_3:55903762-55903781 None:intergenic 35.0%
! GTTCCCAATTTTCTGAATGT+CGG + chr2_3:55903763-55903782 None:intergenic 35.0%
TACTATGTAGACGTAACACT+CGG + chr2_3:55903832-55903851 None:intergenic 35.0%
! TCTTGCGAGTTTTTGTTCAT+GGG - chr2_3:55903854-55903873 Msa0300450:CDS 35.0%
TGGAACTGTGTTCTTTGTAA+TGG - chr2_3:55904483-55904502 Msa0300450:intron 35.0%
GGAACTGTGTTCTTTGTAAT+GGG - chr2_3:55904484-55904503 Msa0300450:intron 35.0%
ATGAATGGGATGTCAACAAA+TGG - chr2_3:55904618-55904637 Msa0300450:intron 35.0%
TGAATGGGATGTCAACAAAT+GGG - chr2_3:55904619-55904638 Msa0300450:intron 35.0%
!! GCTCGAGCATTTTTAAGAAA+TGG - chr2_3:55904813-55904832 Msa0300450:intron 35.0%
CTTCGCATAGAAGAAAACAT+AGG - chr2_3:55904974-55904993 Msa0300450:intron 35.0%
ATACCCATGTATAAGTTAGC+AGG - chr2_3:55905060-55905079 Msa0300450:intron 35.0%
TACCCATGTATAAGTTAGCA+GGG - chr2_3:55905061-55905080 Msa0300450:intron 35.0%
TACCCTGCTAACTTATACAT+GGG + chr2_3:55905066-55905085 None:intergenic 35.0%
TTACCCTGCTAACTTATACA+TGG + chr2_3:55905067-55905086 None:intergenic 35.0%
ATGTATAAGTTAGCAGGGTA+AGG - chr2_3:55905066-55905085 Msa0300450:intron 35.0%
TGTATAAGTTAGCAGGGTAA+GGG - chr2_3:55905067-55905086 Msa0300450:intron 35.0%
! ATTTTGTAGAGGCTTCAAAC+CGG + chr2_3:55905218-55905237 None:intergenic 35.0%
! ACACTCAATGCATTTTGTAG+AGG + chr2_3:55905229-55905248 None:intergenic 35.0%
TCAGAATGTGACTAAAACAC+AGG + chr2_3:55905447-55905466 None:intergenic 35.0%
ACCACTGAGTTTACACTTTA+TGG - chr2_3:55905842-55905861 Msa0300450:CDS 35.0%
TCCATAAAGTGTAAACTCAG+TGG + chr2_3:55905846-55905865 None:intergenic 35.0%
!!! CGTTACCTATTTTTAGCATG+AGG - chr2_3:55905969-55905988 Msa0300450:intron 35.0%
TTGTTGCTGAATAATGATGC+AGG - chr2_3:55906102-55906121 Msa0300450:intron 35.0%
ATACTTCATATGATCGTGCA+AGG + chr2_3:55906199-55906218 None:intergenic 35.0%
! TGCATCCATCTAGCATATTT+TGG - chr2_3:55906218-55906237 Msa0300450:intron 35.0%
GGAAAAACATGATTTCCGTT+TGG + chr2_3:55906296-55906315 None:intergenic 35.0%
AAAGAAAACTGCAGAAGAGA+TGG - chr2_3:55906343-55906362 Msa0300450:intron 35.0%
ATAGGATAGAGATCATGATG+CGG + chr2_3:55906502-55906521 None:intergenic 35.0%
!!! AAGTCCAAGTTTTTTGAGCA+GGG - chr2_3:55906558-55906577 Msa0300450:intron 35.0%
! AATTTTGTAGGTGAGTCTGT+TGG + chr2_3:55906693-55906712 None:intergenic 35.0%
! TCTGTTTGTTAATTGGTTGG+TGG - chr2_3:55906924-55906943 Msa0300450:five_prime_UTR 35.0%
AATAAAGTGACTCAAGAGAG+AGG + chr2_3:55906988-55907007 None:intergenic 35.0%
AAGTACATAACCACTGAATC+TGG + chr2_3:55907119-55907138 None:intergenic 35.0%
! GAAGGTTCGGTTCATAGATT+GGG + chr2_3:55903473-55903492 None:intergenic 40.0%
! TGAAGGTTCGGTTCATAGAT+TGG + chr2_3:55903474-55903493 None:intergenic 40.0%
! ACGGTCTGAAATTGAAGGTT+CGG + chr2_3:55903486-55903505 None:intergenic 40.0%
! ATCTGACGGTCTGAAATTGA+AGG + chr2_3:55903491-55903510 None:intergenic 40.0%
! CGTCTGATTTGAACACCTTT+GGG + chr2_3:55903514-55903533 None:intergenic 40.0%
!! CCAAAGGTGTTCAAATCAGA+CGG - chr2_3:55903512-55903531 Msa0300450:three_prime_UTR 40.0%
!! GTTTTGATCCAGAATCGCTT+CGG - chr2_3:55903543-55903562 Msa0300450:three_prime_UTR 40.0%
!! AGATTTTGGTGGAAGAGAGA+CGG + chr2_3:55903577-55903596 None:intergenic 40.0%
!! TAGGGTTTGGTAGATTTTGG+TGG + chr2_3:55903588-55903607 None:intergenic 40.0%
GAATTTCGGAGATTAGGGTT+TGG + chr2_3:55903601-55903620 None:intergenic 40.0%
GGAATCGAATTTCGGAGATT+AGG + chr2_3:55903607-55903626 None:intergenic 40.0%
GACAGTGAGGAATCGAATTT+CGG + chr2_3:55903615-55903634 None:intergenic 40.0%
ACTATGTAGACGTAACACTC+GGG + chr2_3:55903831-55903850 None:intergenic 40.0%
! GTCTTGCGAGTTTTTGTTCA+TGG - chr2_3:55903853-55903872 Msa0300450:CDS 40.0%
TAAGAAGCTGCACTCATTCT+TGG + chr2_3:55904121-55904140 None:intergenic 40.0%
TATGGTGGTCTCTTATAGGA+TGG + chr2_3:55904298-55904317 None:intergenic 40.0%
GCAATATGGTGGTCTCTTAT+AGG + chr2_3:55904302-55904321 None:intergenic 40.0%
AGGCATACATAGCAATATGG+TGG + chr2_3:55904313-55904332 None:intergenic 40.0%
TTCATTTCAGGGCTGATGAA+TGG - chr2_3:55904603-55904622 Msa0300450:intron 40.0%
TCATTTCAGGGCTGATGAAT+GGG - chr2_3:55904604-55904623 Msa0300450:intron 40.0%
AAAAATGCTCACGGCATTGT+GGG + chr2_3:55905147-55905166 None:intergenic 40.0%
GGTTGACATTGTAACCTCAA+GGG + chr2_3:55905818-55905837 None:intergenic 40.0%
AGGTTGACATTGTAACCTCA+AGG + chr2_3:55905819-55905838 None:intergenic 40.0%
AGTGTAAACTCAGTGGTAAG+AGG + chr2_3:55905839-55905858 None:intergenic 40.0%
GGGTAATCTACATTGGTACA+TGG - chr2_3:55905882-55905901 Msa0300450:CDS 40.0%
AGCAAAACTAAAGCCACACT+CGG + chr2_3:55905934-55905953 None:intergenic 40.0%
TTGCTGAATAATGATGCAGG+TGG - chr2_3:55906105-55906124 Msa0300450:intron 40.0%
! TGCCAATAAAAGCATGTCGA+AGG + chr2_3:55906134-55906153 None:intergenic 40.0%
! CGACATGCTTTTATTGGCAT+AGG - chr2_3:55906135-55906154 Msa0300450:intron 40.0%
CATAAGCCTCTGTTCTTTCT+CGG + chr2_3:55906164-55906183 None:intergenic 40.0%
! CTGCAGTTTTCTTTTTGTCC+AGG + chr2_3:55906337-55906356 None:intergenic 40.0%
TAGGATAGAGATCATGATGC+GGG + chr2_3:55906501-55906520 None:intergenic 40.0%
!!! GAAGTCCAAGTTTTTTGAGC+AGG - chr2_3:55906557-55906576 Msa0300450:intron 40.0%
! GGTGGTGGTAACTTAATACA+TGG + chr2_3:55906630-55906649 None:intergenic 40.0%
TCCTGAAAATCAAAGCACAC+AGG - chr2_3:55906779-55906798 Msa0300450:CDS 40.0%
!! TCCTGTGTGCTTTGATTTTC+AGG + chr2_3:55906783-55906802 None:intergenic 40.0%
TTGTGGATACCTTGTTCATC+TGG - chr2_3:55906870-55906889 Msa0300450:five_prime_UTR 40.0%
AACTGAACACCAGATGAACA+AGG + chr2_3:55906882-55906901 None:intergenic 40.0%
!! GTTGGTGGTTATCTTAGTTG+CGG - chr2_3:55906939-55906958 Msa0300450:five_prime_UTR 40.0%
! TTGGTGGTTATCTTAGTTGC+GGG - chr2_3:55906940-55906959 Msa0300450:five_prime_UTR 40.0%
!! TAGTCATTTGGTGCTGATCA+AGG - chr2_3:55907048-55907067 Msa0300450:five_prime_UTR 40.0%
!! AGTCATTTGGTGCTGATCAA+GGG - chr2_3:55907049-55907068 Msa0300450:five_prime_UTR 40.0%
AGTGAGTGGAGTGATTGCTT+TGG + chr2_3:55903448-55903467 None:intergenic 45.0%
TCATAGATTGGGACAGTGAG+TGG + chr2_3:55903462-55903481 None:intergenic 45.0%
!! TGAACACCTTTGGGATCTGA+CGG + chr2_3:55903505-55903524 None:intergenic 45.0%
! CCGTCTGATTTGAACACCTT+TGG + chr2_3:55903515-55903534 None:intergenic 45.0%
! AAGGTGTTCAAATCAGACGG+TGG - chr2_3:55903515-55903534 Msa0300450:three_prime_UTR 45.0%
GGGGAAGAAATTTGACAGTG+AGG + chr2_3:55903628-55903647 None:intergenic 45.0%
CTTTCTCTGTTTCCGGATCA+TGG - chr2_3:55903719-55903738 Msa0300450:CDS 45.0%
TATCCTCCTTCTCCATGATC+CGG + chr2_3:55903734-55903753 None:intergenic 45.0%
GAATGAGTGCAGCTTCTTAC+AGG - chr2_3:55904122-55904141 Msa0300450:CDS 45.0%
TGCGTTCTTCACAAGCTTTG+TGG - chr2_3:55904463-55904482 Msa0300450:intron 45.0%
GGATGTCAACAAATGGGCAT+GGG - chr2_3:55904625-55904644 Msa0300450:intron 45.0%
TCAACAAATGGGCATGGGAA+GGG - chr2_3:55904630-55904649 Msa0300450:intron 45.0%
CTGTCAATAACAGCTTCCAC+GGG + chr2_3:55904849-55904868 None:intergenic 45.0%
GCTGTCAATAACAGCTTCCA+CGG + chr2_3:55904850-55904869 None:intergenic 45.0%
AAGCTGTTATTGACAGCAGC+AGG - chr2_3:55904853-55904872 Msa0300450:intron 45.0%
TTAGCAGGGTAAGGGTCTAA+GGG - chr2_3:55905075-55905094 Msa0300450:intron 45.0%
AAAATGCTCACGGCATTGTG+GGG + chr2_3:55905146-55905165 None:intergenic 45.0%
GAAAAATGCTCACGGCATTG+TGG + chr2_3:55905148-55905167 None:intergenic 45.0%
! CATTTTGCAACTGTCTCCGA+GGG - chr2_3:55905778-55905797 Msa0300450:intron 45.0%
! ATTTTGCAACTGTCTCCGAG+GGG - chr2_3:55905779-55905798 Msa0300450:intron 45.0%
GATTTGAACTTCGTCCCTTG+AGG - chr2_3:55905801-55905820 Msa0300450:intron 45.0%
!! TGTTGCTTTAATGCCGAGTG+TGG - chr2_3:55905918-55905937 Msa0300450:intron 45.0%
! CGCCTTCGACATGCTTTTAT+TGG - chr2_3:55906129-55906148 Msa0300450:intron 45.0%
AGGATTCCGAGAAAGAACAG+AGG - chr2_3:55906155-55906174 Msa0300450:intron 45.0%
AATCAACTGAGGAGCTTTGC+TGG + chr2_3:55906379-55906398 None:intergenic 45.0%
AGGATAGAGATCATGATGCG+GGG + chr2_3:55906500-55906519 None:intergenic 45.0%
TATTGTATGCAGAGAAGCGG+CGG - chr2_3:55906528-55906547 Msa0300450:intron 45.0%
TGCACCCTGCTCAAAAAACT+TGG + chr2_3:55906565-55906584 None:intergenic 45.0%
! TCTCTCACTCTTTTCTTCCG+AGG + chr2_3:55906595-55906614 None:intergenic 45.0%
TAACAGGTGAACCTGGTGAT+GGG + chr2_3:55906656-55906675 None:intergenic 45.0%
! CAACTTCAGCGCTTTTCTCA+AGG + chr2_3:55906719-55906738 None:intergenic 45.0%
!! TTTGGCGATTTTCAAGCAGC+TGG - chr2_3:55906819-55906838 Msa0300450:five_prime_UTR 45.0%
GGCGCATCTCAATAGTCATT+TGG - chr2_3:55907036-55907055 Msa0300450:five_prime_UTR 45.0%
!! GTCATTTGGTGCTGATCAAG+GGG - chr2_3:55907050-55907069 Msa0300450:five_prime_UTR 45.0%
GCTCAATGAACCAGATTCAG+TGG - chr2_3:55907106-55907125 Msa0300450:five_prime_UTR 45.0%
!!! AATTCAAATTTTTAATTTTT+AGG + chr2_3:55904013-55904032 None:intergenic 5.0%
TTCAGACCGTCAGATCCCAA+AGG - chr2_3:55903496-55903515 Msa0300450:three_prime_UTR 50.0%
CTGTTTCCGGATCATGGAGA+AGG - chr2_3:55903725-55903744 Msa0300450:CDS 50.0%
TTTCCGGATCATGGAGAAGG+AGG - chr2_3:55903728-55903747 Msa0300450:CDS 50.0%
GAAGCTGCACTCATTCTTGG+AGG + chr2_3:55904118-55904137 None:intergenic 50.0%
GGTGGTCTCTTATAGGATGG+TGG + chr2_3:55904295-55904314 None:intergenic 50.0%
GGGATGTCAACAAATGGGCA+TGG - chr2_3:55904624-55904643 Msa0300450:intron 50.0%
GTCAACAAATGGGCATGGGA+AGG - chr2_3:55904629-55904648 Msa0300450:intron 50.0%
ATAACAGCTTCCACGGGATG+TGG + chr2_3:55904843-55904862 None:intergenic 50.0%
GTTAGCAGGGTAAGGGTCTA+AGG - chr2_3:55905074-55905093 Msa0300450:intron 50.0%
! ACGGCATTGTGGGGGATTTT+TGG + chr2_3:55905137-55905156 None:intergenic 50.0%
AAATGCTCACGGCATTGTGG+GGG + chr2_3:55905145-55905164 None:intergenic 50.0%
CCTCGGAGACAGTTGCAAAA+TGG + chr2_3:55905780-55905799 None:intergenic 50.0%
! CCATTTTGCAACTGTCTCCG+AGG - chr2_3:55905777-55905796 Msa0300450:intron 50.0%
GGACGAAGTTCAAATCCCCT+CGG + chr2_3:55905797-55905816 None:intergenic 50.0%
TATGATCGTGCAAGGCAGCT+TGG + chr2_3:55906191-55906210 None:intergenic 50.0%
! AACAGGTGAACCTGGTGATG+GGG + chr2_3:55906655-55906674 None:intergenic 50.0%
GTAACAGGTGAACCTGGTGA+TGG + chr2_3:55906657-55906676 None:intergenic 50.0%
AGTCTGTTGGAGACTTCTGC+GGG + chr2_3:55906680-55906699 None:intergenic 50.0%
GAGTCTGTTGGAGACTTCTG+CGG + chr2_3:55906681-55906700 None:intergenic 50.0%
AAGCTGCACCGAAGCGATTC+TGG + chr2_3:55903554-55903573 None:intergenic 55.0%
AAATGGAGAGCCACATCCCG+TGG - chr2_3:55904830-55904849 Msa0300450:intron 55.0%
GCAGGGTGCAAACAACTCCT+CGG - chr2_3:55906575-55906594 Msa0300450:intron 55.0%
!! ACAGGTGAACCTGGTGATGG+GGG + chr2_3:55906654-55906673 None:intergenic 55.0%
GTCTGTTGGAGACTTCTGCG+GGG + chr2_3:55906679-55906698 None:intergenic 55.0%
TGCGGGGTAACAGGTGAACC+TGG + chr2_3:55906663-55906682 None:intergenic 60.0%
GGAGACTTCTGCGGGGTAAC+AGG + chr2_3:55906672-55906691 None:intergenic 60.0%
!! TTGCGGGCTAGTAGCGTGAG+AGG - chr2_3:55906956-55906975 Msa0300450:five_prime_UTR 60.0%
!! GAACCTGGTGATGGGGGAGG+TGG + chr2_3:55906648-55906667 None:intergenic 65.0%
!! GGTGAACCTGGTGATGGGGG+AGG + chr2_3:55906651-55906670 None:intergenic 65.0%
!! CCTGGTGATGGGGGAGGTGG+TGG + chr2_3:55906645-55906664 None:intergenic 70.0%
CCACCACCTCCCCCATCACC+AGG - chr2_3:55906642-55906661 Msa0300450:intron 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 55903393 55907152 55903393 ID=Msa0300450;Name=Msa0300450
chr2_3 mRNA 55903393 55907152 55903393 ID=Msa0300450-mRNA-1;Parent=Msa0300450;Name=Msa0300450-mRNA-1;_AED=0.02;_eAED=0.02;_QI=345|1|1|1|1|1|8|307|286
chr2_3 exon 55903393 55904005 55903393 ID=Msa0300450-mRNA-1:exon:17879;Parent=Msa0300450-mRNA-1
chr2_3 exon 55904100 55904214 55904100 ID=Msa0300450-mRNA-1:exon:17878;Parent=Msa0300450-mRNA-1
chr2_3 exon 55904331 55904421 55904331 ID=Msa0300450-mRNA-1:exon:17877;Parent=Msa0300450-mRNA-1
chr2_3 exon 55905550 55905581 55905550 ID=Msa0300450-mRNA-1:exon:17876;Parent=Msa0300450-mRNA-1
chr2_3 exon 55905671 55905743 55905671 ID=Msa0300450-mRNA-1:exon:17875;Parent=Msa0300450-mRNA-1
chr2_3 exon 55905832 55905932 55905832 ID=Msa0300450-mRNA-1:exon:17874;Parent=Msa0300450-mRNA-1
chr2_3 exon 55906402 55906433 55906402 ID=Msa0300450-mRNA-1:exon:17873;Parent=Msa0300450-mRNA-1
chr2_3 exon 55906697 55907152 55906697 ID=Msa0300450-mRNA-1:exon:17872;Parent=Msa0300450-mRNA-1
chr2_3 five_prime_UTR 55906808 55907152 55906808 ID=Msa0300450-mRNA-1:five_prime_utr;Parent=Msa0300450-mRNA-1
chr2_3 CDS 55906697 55906807 55906697 ID=Msa0300450-mRNA-1:cds;Parent=Msa0300450-mRNA-1
chr2_3 CDS 55906402 55906433 55906402 ID=Msa0300450-mRNA-1:cds;Parent=Msa0300450-mRNA-1
chr2_3 CDS 55905832 55905932 55905832 ID=Msa0300450-mRNA-1:cds;Parent=Msa0300450-mRNA-1
chr2_3 CDS 55905671 55905743 55905671 ID=Msa0300450-mRNA-1:cds;Parent=Msa0300450-mRNA-1
chr2_3 CDS 55905550 55905581 55905550 ID=Msa0300450-mRNA-1:cds;Parent=Msa0300450-mRNA-1
chr2_3 CDS 55904331 55904421 55904331 ID=Msa0300450-mRNA-1:cds;Parent=Msa0300450-mRNA-1
chr2_3 CDS 55904100 55904214 55904100 ID=Msa0300450-mRNA-1:cds;Parent=Msa0300450-mRNA-1
chr2_3 CDS 55903700 55904005 55903700 ID=Msa0300450-mRNA-1:cds;Parent=Msa0300450-mRNA-1
chr2_3 three_prime_UTR 55903393 55903699 55903393 ID=Msa0300450-mRNA-1:three_prime_utr;Parent=Msa0300450-mRNA-1
Gene Sequence

>Msa0300450

ATGGAGAAGGAGGATAACAAACCCGACATTCAGAAAATTGGGAACGAAATTGAAGATACTGAACCCATAGAACTCGTTCTTTTTCAAGTTCCCGAGTGTTACGTCTACATAATACCTCCAAGAATGAGTGCAGCTTCTTACAGGGCTGATGAATGGGATGTCAACAAATGGGCATGGGAAGGGATATTGAAAGTTGTTAGCAAGGGAGAAGAATGTATCATAAAACTAGAAGATAAGAACACAGGTGAATTATATGCTCGAGCATTTTTAAGAAATGGAGAGCCACATCCCGTGGAAGCTGTTATTGACAGCAGCAGATATTTTGTTCTTCGCATAGAAGAAAACATAGGTGGTCGCCTTCGACATGCTTTTATTGGCATAGGATTCCGAGAAAGAACAGAGGCTTATGACTTCCAAGCTGCCTTGCACGATCATATGAAATACCTGGACAAAAAGAAAACTGCAGAAGAGATGGAACAACATTACCAGCAAAGCTCCTCAGTTGATTACAGTTTGAAAGAAGGGGAGACTCTTGTGCTGCAAATCAAGAACAATAGAAGCGGCGGCAATGTGAAGTCCAAGTTTTTTGAGCAGGGTGCAAACAACTCCTCGGAAGAAAAGAGTGAGAGAAAAGAATCTATACCATGTATTAAGTTACCACCACCTCCCCCATCACCAGGTTCACCTGTTACCCCGCAGAAGTCTCCAACAGACTCACCTACAAAATTAAGCCTTGAGAAAAGCGCTGAAGTTGAGACCTCTAAAATAGTTAAAGAAACAGAACATGAAAAATCTCCTGAAAATCAAAGCACACAGGATGTAGTAGATGATGATTTTGGCGATTTTCAAGCAGCTGGTTAA
Protein sequence

>Msa0300450

MEKEDNKPDIQKIGNEIEDTEPIELVLFQVPECYVYIIPPRMSAASYRADEWDVNKWAWEGILKVVSKGEECIIKLEDKNTGELYARAFLRNGEPHPVEAVIDSSRYFVLRIEENIGGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLDKKKTAEEMEQHYQQSSSVDYSLKEGETLVLQIKNNRSGGNVKSKFFEQGANNSSEEKSERKESIPCIKLPPPPPSPGSPVTPQKSPTDSPTKLSLEKSAEVETSKIVKETEHEKSPENQSTQDVVDDDFGDFQAAG*