AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0221160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221160 MtrunA17_Chr2g0325501 98.258 287 4 1 1 286 1 287 0.0 578
Msa0221160 MtrunA17_Chr1g0183691 35.600 250 142 5 18 259 5 243 1.99e-41 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221160 AT3G58600.1 66.787 277 83 4 18 286 27 302 2.15e-114 332
Msa0221160 AT1G03900.1 44.311 167 84 3 23 183 12 175 4.38e-39 138

Find 102 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGAAATCTTTCTCTGTTTC+CGG 0.158594 2_1:-69763828 Msa0221160:five_prime_UTR
GTCTTCTGAAGATATGAATT+TGG 0.165545 2_1:+69763894 None:intergenic
AAACCCGACATTCAGAAAAT+TGG 0.187508 2_1:-69763784 Msa0221160:CDS
AAGCTGCACCGAAGCGATTC+TGG 0.249439 2_1:+69763989 None:intergenic
GTCCATATCTGTTTGTTAAT+TGG 0.252680 2_1:-69760623 Msa0221160:three_prime_UTR
GATTAGGGTTTGGTAGATTT+TGG 0.253778 2_1:+69763952 None:intergenic
GAATGAGTGCAGCTTCTTAC+AGG 0.257166 2_1:-69763418 Msa0221160:intron
TCTTCTGAAGATATGAATTT+GGG 0.287985 2_1:+69763895 None:intergenic
CCACAATCATTCATAATAGA+TGG 0.298169 2_1:+69760687 None:intergenic
CCGTCTGATTTGAACACCTT+TGG 0.302053 2_1:+69764028 None:intergenic
CTTTCTCTGTTTCCGGATCA+TGG 0.307728 2_1:-69763821 Msa0221160:exon
GAGAAAGATTTCGAATTTGA+TGG 0.310318 2_1:+69763837 None:intergenic
GACAGTGAGGAATCGAATTT+CGG 0.321190 2_1:+69763928 None:intergenic
TAAGAAGCTGCACTCATTCT+TGG 0.323176 2_1:+69763422 None:intergenic
GAATCGAATTTCGGAGATTA+GGG 0.329332 2_1:+69763937 None:intergenic
GGAATCGAATTTCGGAGATT+AGG 0.349872 2_1:+69763936 None:intergenic
CATAAGCCTCTGTTCTTTCT+CGG 0.358789 2_1:+69761379 None:intergenic
CGTCTGATTTGAACACCTTT+GGG 0.362526 2_1:+69764029 None:intergenic
AATCAACTGAGGAGCTTTGC+TGG 0.368512 2_1:+69761164 None:intergenic
GGAGACTTCTGCGGGGTAAC+AGG 0.373330 2_1:+69760871 None:intergenic
TGAAGGTTCGGTTCATAGAT+TGG 0.375896 2_1:+69764069 None:intergenic
GATGTAGTAGATGATGATTT+TGG 0.381931 2_1:-69760739 Msa0221160:CDS
TTGGTGGTTATCTTAGTTGC+GGG 0.385750 2_1:-69760600 Msa0221160:three_prime_UTR
AACCAATTAACAAACAGATA+TGG 0.402496 2_1:+69760621 None:intergenic
AAGTACATAACCACTGAATC+TGG 0.406019 2_1:+69760424 None:intergenic
GGCGCATCTCAATAGTCATT+TGG 0.409262 2_1:-69760504 Msa0221160:three_prime_UTR
TTCATTTCAGGGCTGATGAA+TGG 0.412505 2_1:-69762937 Msa0221160:intron
AATGAAGAACAAAAGCGATC+TGG 0.425569 2_1:+69763871 None:intergenic
TTGTGGATACCTTGTTCATC+TGG 0.437607 2_1:-69760670 Msa0221160:three_prime_UTR
GAAGGTTCGGTTCATAGATT+GGG 0.439722 2_1:+69764070 None:intergenic
TAGTCATTTGGTGCTGATCA+AGG 0.444839 2_1:-69760492 Msa0221160:three_prime_UTR
ACGGTCTGAAATTGAAGGTT+CGG 0.449625 2_1:+69764057 None:intergenic
TCATTTCAGGGCTGATGAAT+GGG 0.449823 2_1:-69762936 Msa0221160:intron
AACCCGACATTCAGAAAATT+GGG 0.462440 2_1:-69763783 Msa0221160:CDS
TTGATTACAGTTTGAAAGAA+GGG 0.463667 2_1:-69761147 Msa0221160:CDS
GATATTGAAAGTTGTTAGCA+AGG 0.466773 2_1:-69762888 Msa0221160:CDS
ATGAATGGGATGTCAACAAA+TGG 0.473047 2_1:-69762922 Msa0221160:CDS
GTTGATTACAGTTTGAAAGA+AGG 0.478319 2_1:-69761148 Msa0221160:CDS
ATATCTGTTTGTTAATTGGT+TGG 0.480576 2_1:-69760619 Msa0221160:three_prime_UTR
AGTGAGTGGAGTGATTGCTT+TGG 0.481595 2_1:+69764095 None:intergenic
TAACAGGTGAACCTGGTGAT+GGG 0.483068 2_1:+69760887 None:intergenic
TATCCTCCTTCTCCATGATC+CGG 0.493809 2_1:+69763809 None:intergenic
ATGAAGAACAAAAGCGATCT+GGG 0.497981 2_1:+69763872 None:intergenic
GTTGGTGGTTATCTTAGTTG+CGG 0.506423 2_1:-69760601 Msa0221160:three_prime_UTR
ATAACAGCTTCCACGGGATG+TGG 0.507930 2_1:+69762700 None:intergenic
AGTCTGTTGGAGACTTCTGC+GGG 0.508559 2_1:+69760863 None:intergenic
GAATTTCGGAGATTAGGGTT+TGG 0.508940 2_1:+69763942 None:intergenic
GATTTCGAATTTGATGGAAG+GGG 0.511303 2_1:+69763843 None:intergenic
TCTGTTTGTTAATTGGTTGG+TGG 0.513135 2_1:-69760616 Msa0221160:three_prime_UTR
AAGCTGTTATTGACAGCAGC+AGG 0.513305 2_1:-69762687 Msa0221160:intron
AGATTTCGAATTTGATGGAA+GGG 0.516307 2_1:+69763842 None:intergenic
GTCAACAAATGGGCATGGGA+AGG 0.517214 2_1:-69762911 Msa0221160:CDS
ATCTGACGGTCTGAAATTGA+AGG 0.520220 2_1:+69764052 None:intergenic
TGCCAATAAAAGCGTGTCGA+AGG 0.520429 2_1:+69761409 None:intergenic
AAAGAAAACTGCAGAAGAGA+TGG 0.526513 2_1:-69761197 Msa0221160:CDS
TGCGGGGTAACAGGTGAACC+TGG 0.526812 2_1:+69760880 None:intergenic
AAGATTTCGAATTTGATGGA+AGG 0.527298 2_1:+69763841 None:intergenic
TATGATCGTGCAAGGCAGCT+TGG 0.527856 2_1:+69761352 None:intergenic
AGTCATTTGGTGCTGATCAA+GGG 0.533892 2_1:-69760491 Msa0221160:three_prime_UTR
GTAACAGGTGAACCTGGTGA+TGG 0.537068 2_1:+69760886 None:intergenic
CCATCTATTATGAATGATTG+TGG 0.537988 2_1:-69760687 Msa0221160:three_prime_UTR
CACAATCATTCATAATAGAT+GGG 0.543332 2_1:+69760688 None:intergenic
AACAGGTGAACCTGGTGATG+GGG 0.546112 2_1:+69760888 None:intergenic
TGAACACCTTTGGGATCTGA+CGG 0.548857 2_1:+69764038 None:intergenic
TCATAGATTGGGACAGTGAG+TGG 0.555654 2_1:+69764081 None:intergenic
GAAGCTGCACTCATTCTTGG+AGG 0.557836 2_1:+69763425 None:intergenic
CTGTTTCCGGATCATGGAGA+AGG 0.558980 2_1:-69763815 Msa0221160:exon
GAGTCTGTTGGAGACTTCTG+CGG 0.568129 2_1:+69760862 None:intergenic
ATATTGAAAGTTGTTAGCAA+GGG 0.568944 2_1:-69762887 Msa0221160:CDS
TCAACAAATGGGCATGGGAA+GGG 0.569352 2_1:-69762910 Msa0221160:CDS
TGATTACAGTTTGAAAGAAG+GGG 0.573861 2_1:-69761146 Msa0221160:CDS
TACTATGTAGACGTAACACT+CGG 0.574408 2_1:+69763711 None:intergenic
GGGGAAGAAATTTGACAGTG+AGG 0.575596 2_1:+69763915 None:intergenic
GGGATGTCAACAAATGGGCA+TGG 0.576412 2_1:-69762916 Msa0221160:CDS
GCTGTCAATAACAGCTTCCA+CGG 0.579429 2_1:+69762693 None:intergenic
GTCATTTGGTGCTGATCAAG+GGG 0.594704 2_1:-69760490 Msa0221160:three_prime_UTR
CTGTCAATAACAGCTTCCAC+GGG 0.598765 2_1:+69762694 None:intergenic
GCTCAATGAACCAGATTCAG+TGG 0.598785 2_1:-69760434 Msa0221160:three_prime_UTR
GGATGTCAACAAATGGGCAT+GGG 0.603400 2_1:-69762915 Msa0221160:CDS
TGAATGGGATGTCAACAAAT+GGG 0.604897 2_1:-69762921 Msa0221160:CDS
AAATGGAGAGCCACATCCCG+TGG 0.605045 2_1:-69762710 Msa0221160:CDS
CTTCGCATAGAAGAAAACAT+AGG 0.607237 2_1:-69762566 Msa0221160:intron
ACAGGTGAACCTGGTGATGG+GGG 0.607450 2_1:+69760889 None:intergenic
CCAAAGGTGTTCAAATCAGA+CGG 0.609487 2_1:-69764028 Msa0221160:five_prime_UTR
GGTGGTGGTAACTTAATACA+TGG 0.613088 2_1:+69760913 None:intergenic
TATTGTATGCAGAGAAGCGG+CGG 0.616671 2_1:-69761012 Msa0221160:intron
ATTTATTGTATGCAGAGAAG+CGG 0.617754 2_1:-69761015 Msa0221160:intron
TTGCGGGCTAGTAGCGTGAG+AGG 0.618533 2_1:-69760584 Msa0221160:three_prime_UTR
TTTCAAACTGTAATCAACTG+AGG 0.625498 2_1:+69761153 None:intergenic
AATAAAGTGACTCAAGAGAG+AGG 0.633889 2_1:+69760555 None:intergenic
GCAGGGTGCAAACAACTCCT+CGG 0.636828 2_1:-69760965 Msa0221160:CDS
ATACTTCATATGATCGTGCA+AGG 0.645577 2_1:+69761344 None:intergenic
TTTCCGGATCATGGAGAAGG+AGG 0.645908 2_1:-69763812 Msa0221160:exon
TCCTGAAAATCAAAGCACAC+AGG 0.648603 2_1:-69760761 Msa0221160:CDS
ACTATGTAGACGTAACACTC+GGG 0.657792 2_1:+69763712 None:intergenic
AACTGAACACCAGATGAACA+AGG 0.660378 2_1:+69760661 None:intergenic
CTTCTGAAGATATGAATTTG+GGG 0.667262 2_1:+69763896 None:intergenic
AAACTAGAAGATAAGAACAC+AGG 0.671758 2_1:-69762848 Msa0221160:intron
TTCAGACCGTCAGATCCCAA+AGG 0.692418 2_1:-69764044 Msa0221160:five_prime_UTR
GTCTGTTGGAGACTTCTGCG+GGG 0.741687 2_1:+69760864 None:intergenic
AAGGTGTTCAAATCAGACGG+TGG 0.746634 2_1:-69764025 Msa0221160:five_prime_UTR
AGGATTCCGAGAAAGAACAG+AGG 0.756772 2_1:-69761385 Msa0221160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGAATTTGATTTTTTTTT+TGG - chr2_1:69761072-69761091 Msa0221160:intron 10.0%
!!! CGATTTTTTTTTTTAAATAT+CGG + chr2_1:69762667-69762686 None:intergenic 10.0%
!! CGATATTTAAAAAAAAAAAT+CGG - chr2_1:69762665-69762684 Msa0221160:intron 10.0%
!! GATATTTAAAAAAAAAAATC+GGG - chr2_1:69762666-69762685 Msa0221160:intron 10.0%
!! ATATTTAAAAAAAAAAATCG+GGG - chr2_1:69762667-69762686 Msa0221160:intron 10.0%
!! TCCAAAATTAAAAAAAAAAT+CGG - chr2_1:69762723-69762742 Msa0221160:CDS 10.0%
!! CAAATATTACTATTTAATAT+GGG + chr2_1:69763054-69763073 None:intergenic 10.0%
!! ACAAATATTACTATTTAATA+TGG + chr2_1:69763055-69763074 None:intergenic 10.0%
!!! TTTCTTTGTTTTGTTATTTT+AGG - chr2_1:69760413-69760432 Msa0221160:three_prime_UTR 15.0%
!!! CAAGATTAAATTTTCATTTT+GGG + chr2_1:69760989-69761008 None:intergenic 15.0%
!!! TAAATGAAGTATTTTTAACT+AGG - chr2_1:69761797-69761816 Msa0221160:intron 15.0%
!! CCAAAATTAAAAAAAAAATC+GGG - chr2_1:69762724-69762743 Msa0221160:CDS 15.0%
!! CAAAATTAAAAAAAAAATCG+GGG - chr2_1:69762725-69762744 Msa0221160:CDS 15.0%
!! TTCTTATTAATTATGAAGTT+TGG - chr2_1:69763093-69763112 Msa0221160:intron 15.0%
!!! TTCTTTTTATTTTTTCCAAA+CGG - chr2_1:69763295-69763314 Msa0221160:intron 15.0%
!!! TTTGTTTTGTTATTTTAGGT+AGG - chr2_1:69760417-69760436 Msa0221160:three_prime_UTR 20.0%
!!! TTGTTTTGTTATTTTAGGTA+GGG - chr2_1:69760418-69760437 Msa0221160:three_prime_UTR 20.0%
!! AATAGTATGTGATAGAAAAA+TGG - chr2_1:69760918-69760937 Msa0221160:CDS 20.0%
!! TTGATTAATTCAATTGACAA+TGG - chr2_1:69760958-69760977 Msa0221160:CDS 20.0%
!!! GCAAGATTAAATTTTCATTT+TGG + chr2_1:69760990-69761009 None:intergenic 20.0%
!! ATTTAATGTTAGTGATTAGA+TGG - chr2_1:69761047-69761066 Msa0221160:intron 20.0%
!! TTTAATGTTAGTGATTAGAT+GGG - chr2_1:69761048-69761067 Msa0221160:intron 20.0%
!! TAAAGAAGTGGTTATAAAAT+AGG + chr2_1:69761350-69761369 None:intergenic 20.0%
!!! TAGTAGTTATTTTAAAGAAG+TGG + chr2_1:69761362-69761381 None:intergenic 20.0%
!! AAAATGCATTAAAAGTAAAG+AGG + chr2_1:69761442-69761461 None:intergenic 20.0%
!! AAAAAAACAAGTTCATAAAC+TGG + chr2_1:69761545-69761564 None:intergenic 20.0%
!!! TATTATTCCTTTTTGTACTT+AGG - chr2_1:69761927-69761946 Msa0221160:intron 20.0%
!! TAATTTAAGAAAAATGCTCA+CGG + chr2_1:69762173-69762192 None:intergenic 20.0%
!!! ATTCCATTATATTTTGTTCA+AGG + chr2_1:69762208-69762227 None:intergenic 20.0%
!!! AAAATATAATGGAATTTAGC+CGG - chr2_1:69762213-69762232 Msa0221160:intron 20.0%
!! TAAACATGAACAAAAATTTC+AGG - chr2_1:69762493-69762512 Msa0221160:intron 20.0%
!!! CCCGATTTTTTTTTTAATTT+TGG + chr2_1:69762727-69762746 None:intergenic 20.0%
!!! TGTTTCTTTAACTATTTTAG+AGG + chr2_1:69763762-69763781 None:intergenic 20.0%
!!! TGTTTTGTTATTTTAGGTAG+GGG - chr2_1:69760419-69760438 Msa0221160:three_prime_UTR 25.0%
!! TCTTCTGAAGATATGAATTT+GGG + chr2_1:69760665-69760684 None:intergenic 25.0%
!! TTAGGTGCACAATAATTTTT+GGG + chr2_1:69761012-69761031 None:intergenic 25.0%
!! TTTAGGTGCACAATAATTTT+TGG + chr2_1:69761013-69761032 None:intergenic 25.0%
! TTAATGTTAGTGATTAGATG+GGG - chr2_1:69761049-69761068 Msa0221160:intron 25.0%
! GATGTTATTAATGTTCATGA+TGG - chr2_1:69761222-69761241 Msa0221160:intron 25.0%
!! TTTTAATGAATATGCGTTGA+CGG - chr2_1:69761269-69761288 Msa0221160:intron 25.0%
! ACTAAATGCTCAATTCTTAT+TGG - chr2_1:69761378-69761397 Msa0221160:CDS 25.0%
! ATATATGTGAAGTTCATTTC+AGG - chr2_1:69761608-69761627 Msa0221160:intron 25.0%
! TATATGTGAAGTTCATTTCA+GGG - chr2_1:69761609-69761628 Msa0221160:intron 25.0%
! ATATTGAAAGTTGTTAGCAA+GGG - chr2_1:69761670-69761689 Msa0221160:intron 25.0%
! AATGCATTGAGTGTTTATTA+TGG - chr2_1:69762252-69762271 Msa0221160:intron 25.0%
! TCAGATATATGATTGAAAGT+GGG + chr2_1:69762319-69762338 None:intergenic 25.0%
! ATCAGATATATGATTGAAAG+TGG + chr2_1:69762320-69762339 None:intergenic 25.0%
! ATGAGAACAAAAATACTAGA+AGG - chr2_1:69762393-69762412 Msa0221160:intron 25.0%
!! TATTTGTTTGAGATCGATAT+AGG - chr2_1:69762434-69762453 Msa0221160:intron 25.0%
! ATGGAACCAAATATCATATT+AGG - chr2_1:69762878-69762897 Msa0221160:CDS 25.0%
! TGGAACCAAATATCATATTA+GGG - chr2_1:69762879-69762898 Msa0221160:CDS 25.0%
! GATTACCCTAATATGATATT+TGG + chr2_1:69762887-69762906 None:intergenic 25.0%
! TAAATCCAAAATATGCTAGA+TGG + chr2_1:69763243-69763262 None:intergenic 25.0%
! AGGTATCTTCAAAACAATTT+AGG + chr2_1:69763334-69763353 None:intergenic 25.0%
!!! TAAATTGTTTTGAAGATACC+TGG - chr2_1:69763333-69763352 Msa0221160:intron 25.0%
! TTGATTACAGTTTGAAAGAA+GGG - chr2_1:69763410-69763429 Msa0221160:intron 25.0%
! CACAATCATTCATAATAGAT+GGG + chr2_1:69763872-69763891 None:intergenic 25.0%
! AACCAATTAACAAACAGATA+TGG + chr2_1:69763939-69763958 None:intergenic 25.0%
!! ATATCTGTTTGTTAATTGGT+TGG - chr2_1:69763938-69763957 Msa0221160:five_prime_UTR 25.0%
!!! TATGCTTTGTTTTCATTTCT+TGG - chr2_1:69764032-69764051 Msa0221160:five_prime_UTR 25.0%
! CTTCTGAAGATATGAATTTG+GGG + chr2_1:69760664-69760683 None:intergenic 30.0%
! GTCTTCTGAAGATATGAATT+TGG + chr2_1:69760666-69760685 None:intergenic 30.0%
AGATTTCGAATTTGATGGAA+GGG + chr2_1:69760718-69760737 None:intergenic 30.0%
AAGATTTCGAATTTGATGGA+AGG + chr2_1:69760719-69760738 None:intergenic 30.0%
GAGAAAGATTTCGAATTTGA+TGG + chr2_1:69760723-69760742 None:intergenic 30.0%
TGAAAAAGAACGAGTTCTAT+GGG + chr2_1:69760821-69760840 None:intergenic 30.0%
TTGAAAAAGAACGAGTTCTA+TGG + chr2_1:69760822-69760841 None:intergenic 30.0%
!!! TTTTTTTGGTGTCAAGCTAA+TGG - chr2_1:69761086-69761105 Msa0221160:intron 30.0%
!!! TTTTTTGGTGTCAAGCTAAT+GGG - chr2_1:69761087-69761106 Msa0221160:intron 30.0%
TTGTTTGTTCATCTTAAGCT+TGG - chr2_1:69761195-69761214 Msa0221160:CDS 30.0%
AATAGGCATACATAGCAATA+TGG + chr2_1:69761333-69761352 None:intergenic 30.0%
GATATTGAAAGTTGTTAGCA+AGG - chr2_1:69761669-69761688 Msa0221160:intron 30.0%
AAACTAGAAGATAAGAACAC+AGG - chr2_1:69761709-69761728 Msa0221160:intron 30.0%
!! AGGTTATTTTCCATAACTGT+TGG - chr2_1:69761890-69761909 Msa0221160:intron 30.0%
CTAAAATCTACCAACAGTTA+TGG + chr2_1:69761903-69761922 None:intergenic 30.0%
TAAGCATCCTAAGTACAAAA+AGG + chr2_1:69761937-69761956 None:intergenic 30.0%
TAAAACAACTACAACCTGTT+TGG - chr2_1:69762121-69762140 Msa0221160:intron 30.0%
!!! TTTTTGGAACTAAACCAAAC+AGG + chr2_1:69762138-69762157 None:intergenic 30.0%
! CCATTATATTTTGTTCAAGG+TGG + chr2_1:69762205-69762224 None:intergenic 30.0%
CCACCTTGAACAAAATATAA+TGG - chr2_1:69762202-69762221 Msa0221160:intron 30.0%
! GTGTTAGTTTCGAAAATTCA+AGG + chr2_1:69762370-69762389 None:intergenic 30.0%
AAAATAGCAATGAACACTGA+TGG - chr2_1:69762629-69762648 Msa0221160:intron 30.0%
AAAAAAAAAATCGGGGAAAG+AGG - chr2_1:69762674-69762693 Msa0221160:intron 30.0%
AAAAAAAAAATCGGGGAAAG+AGG - chr2_1:69762674-69762693 Msa0221160:intron 30.0%
! CAGTTGCAAAATGGTATTTT+GGG + chr2_1:69762788-69762807 None:intergenic 30.0%
! ACAGTTGCAAAATGGTATTT+TGG + chr2_1:69762789-69762808 None:intergenic 30.0%
CATATTAGGGTAATCTACAT+TGG - chr2_1:69762892-69762911 Msa0221160:CDS 30.0%
AACTTCCTCATGCTAAAAAT+AGG + chr2_1:69762994-69763013 None:intergenic 30.0%
!!! ATTTTTAGCATGAGGAAGTT+TGG - chr2_1:69762994-69763013 Msa0221160:intron 30.0%
!! GAAGTTTGGACATTTTAAGT+TGG - chr2_1:69763008-69763027 Msa0221160:intron 30.0%
TTTCAAACTGTAATCAACTG+AGG + chr2_1:69763407-69763426 None:intergenic 30.0%
GTTGATTACAGTTTGAAAGA+AGG - chr2_1:69763409-69763428 Msa0221160:intron 30.0%
TGATTACAGTTTGAAAGAAG+GGG - chr2_1:69763411-69763430 Msa0221160:intron 30.0%
CTGCATACAATAAATAGGAT+AGG + chr2_1:69763537-69763556 None:intergenic 30.0%
CTTCTCTGCATACAATAAAT+AGG + chr2_1:69763542-69763561 None:intergenic 30.0%
ATTTATTGTATGCAGAGAAG+CGG - chr2_1:69763542-69763561 Msa0221160:intron 30.0%
! TTCTCAAGGCTTAATTTTGT+AGG + chr2_1:69763722-69763741 None:intergenic 30.0%
! GATGTAGTAGATGATGATTT+TGG - chr2_1:69763818-69763837 Msa0221160:exon 30.0%
CCACAATCATTCATAATAGA+TGG + chr2_1:69763873-69763892 None:intergenic 30.0%
CCATCTATTATGAATGATTG+TGG - chr2_1:69763870-69763889 Msa0221160:five_prime_UTR 30.0%
! GTCCATATCTGTTTGTTAAT+TGG - chr2_1:69763934-69763953 Msa0221160:five_prime_UTR 30.0%
!! GATTAGGGTTTGGTAGATTT+TGG + chr2_1:69760608-69760627 None:intergenic 35.0%
GAATCGAATTTCGGAGATTA+GGG + chr2_1:69760623-69760642 None:intergenic 35.0%
ATGAAGAACAAAAGCGATCT+GGG + chr2_1:69760688-69760707 None:intergenic 35.0%
AATGAAGAACAAAAGCGATC+TGG + chr2_1:69760689-69760708 None:intergenic 35.0%
GATTTCGAATTTGATGGAAG+GGG + chr2_1:69760717-69760736 None:intergenic 35.0%
TCGAAATCTTTCTCTGTTTC+CGG - chr2_1:69760729-69760748 Msa0221160:CDS 35.0%
AAACCCGACATTCAGAAAAT+TGG - chr2_1:69760773-69760792 Msa0221160:CDS 35.0%
AACCCGACATTCAGAAAATT+GGG - chr2_1:69760774-69760793 Msa0221160:CDS 35.0%
! TTCCCAATTTTCTGAATGTC+GGG + chr2_1:69760779-69760798 None:intergenic 35.0%
! GTTCCCAATTTTCTGAATGT+CGG + chr2_1:69760780-69760799 None:intergenic 35.0%
TACTATGTAGACGTAACACT+CGG + chr2_1:69760849-69760868 None:intergenic 35.0%
! TCTTGCGAGTTTTTGTTCAT+GGG - chr2_1:69760871-69760890 Msa0221160:CDS 35.0%
TGGAACTGTGTTCTTTGTAA+TGG - chr2_1:69761500-69761519 Msa0221160:intron 35.0%
GGAACTGTGTTCTTTGTAAT+GGG - chr2_1:69761501-69761520 Msa0221160:intron 35.0%
ATGAATGGGATGTCAACAAA+TGG - chr2_1:69761635-69761654 Msa0221160:intron 35.0%
TGAATGGGATGTCAACAAAT+GGG - chr2_1:69761636-69761655 Msa0221160:intron 35.0%
!! GCTCGAGCATTTTTAAGAAA+TGG - chr2_1:69761830-69761849 Msa0221160:intron 35.0%
CTTCGCATAGAAGAAAACAT+AGG - chr2_1:69761991-69762010 Msa0221160:intron 35.0%
ATACCCATGTATAAGTTAGC+AGG - chr2_1:69762077-69762096 Msa0221160:intron 35.0%
TACCCATGTATAAGTTAGCA+GGG - chr2_1:69762078-69762097 Msa0221160:intron 35.0%
TACCCTGCTAACTTATACAT+GGG + chr2_1:69762083-69762102 None:intergenic 35.0%
TTACCCTGCTAACTTATACA+TGG + chr2_1:69762084-69762103 None:intergenic 35.0%
ATGTATAAGTTAGCAGGGTA+AGG - chr2_1:69762083-69762102 Msa0221160:intron 35.0%
TGTATAAGTTAGCAGGGTAA+GGG - chr2_1:69762084-69762103 Msa0221160:intron 35.0%
! ATTTTGTAGAGGCTTCAAAC+CGG + chr2_1:69762235-69762254 None:intergenic 35.0%
! ACACTCAATGCATTTTGTAG+AGG + chr2_1:69762246-69762265 None:intergenic 35.0%
TCAGAATGTGACTAAAACAC+AGG + chr2_1:69762464-69762483 None:intergenic 35.0%
ACCACTGAGTTTACACTTTA+TGG - chr2_1:69762859-69762878 Msa0221160:CDS 35.0%
TCCATAAAGTGTAAACTCAG+TGG + chr2_1:69762863-69762882 None:intergenic 35.0%
!!! CGTTACCTATTTTTAGCATG+AGG - chr2_1:69762986-69763005 Msa0221160:intron 35.0%
TTGTTGCTGAATAATGATGC+AGG - chr2_1:69763119-69763138 Msa0221160:intron 35.0%
ATACTTCATATGATCGTGCA+AGG + chr2_1:69763216-69763235 None:intergenic 35.0%
! TGCATCCATCTAGCATATTT+TGG - chr2_1:69763235-69763254 Msa0221160:intron 35.0%
GGAAAAACATGATTTCCGTT+TGG + chr2_1:69763313-69763332 None:intergenic 35.0%
AAAGAAAACTGCAGAAGAGA+TGG - chr2_1:69763360-69763379 Msa0221160:intron 35.0%
ATAGGATAGAGATCATGATG+CGG + chr2_1:69763519-69763538 None:intergenic 35.0%
!!! AAGTCCAAGTTTTTTGAGCA+GGG - chr2_1:69763575-69763594 Msa0221160:intron 35.0%
! AATTTTGTAGGTGAGTCTGT+TGG + chr2_1:69763710-69763729 None:intergenic 35.0%
! TCTGTTTGTTAATTGGTTGG+TGG - chr2_1:69763941-69763960 Msa0221160:five_prime_UTR 35.0%
AATAAAGTGACTCAAGAGAG+AGG + chr2_1:69764005-69764024 None:intergenic 35.0%
AAGTACATAACCACTGAATC+TGG + chr2_1:69764136-69764155 None:intergenic 35.0%
! GAAGGTTCGGTTCATAGATT+GGG + chr2_1:69760490-69760509 None:intergenic 40.0%
! TGAAGGTTCGGTTCATAGAT+TGG + chr2_1:69760491-69760510 None:intergenic 40.0%
! ACGGTCTGAAATTGAAGGTT+CGG + chr2_1:69760503-69760522 None:intergenic 40.0%
! ATCTGACGGTCTGAAATTGA+AGG + chr2_1:69760508-69760527 None:intergenic 40.0%
! CGTCTGATTTGAACACCTTT+GGG + chr2_1:69760531-69760550 None:intergenic 40.0%
!! CCAAAGGTGTTCAAATCAGA+CGG - chr2_1:69760529-69760548 Msa0221160:three_prime_UTR 40.0%
!! GTTTTGATCCAGAATCGCTT+CGG - chr2_1:69760560-69760579 Msa0221160:three_prime_UTR 40.0%
!! AGATTTTGGTGGAAGAGAGA+CGG + chr2_1:69760594-69760613 None:intergenic 40.0%
!! TAGGGTTTGGTAGATTTTGG+TGG + chr2_1:69760605-69760624 None:intergenic 40.0%
GAATTTCGGAGATTAGGGTT+TGG + chr2_1:69760618-69760637 None:intergenic 40.0%
GGAATCGAATTTCGGAGATT+AGG + chr2_1:69760624-69760643 None:intergenic 40.0%
GACAGTGAGGAATCGAATTT+CGG + chr2_1:69760632-69760651 None:intergenic 40.0%
ACTATGTAGACGTAACACTC+GGG + chr2_1:69760848-69760867 None:intergenic 40.0%
! GTCTTGCGAGTTTTTGTTCA+TGG - chr2_1:69760870-69760889 Msa0221160:CDS 40.0%
TAAGAAGCTGCACTCATTCT+TGG + chr2_1:69761138-69761157 None:intergenic 40.0%
TATGGTGGTCTCTTATAGGA+TGG + chr2_1:69761315-69761334 None:intergenic 40.0%
GCAATATGGTGGTCTCTTAT+AGG + chr2_1:69761319-69761338 None:intergenic 40.0%
AGGCATACATAGCAATATGG+TGG + chr2_1:69761330-69761349 None:intergenic 40.0%
TTCATTTCAGGGCTGATGAA+TGG - chr2_1:69761620-69761639 Msa0221160:intron 40.0%
TCATTTCAGGGCTGATGAAT+GGG - chr2_1:69761621-69761640 Msa0221160:intron 40.0%
AAAAATGCTCACGGCATTGT+GGG + chr2_1:69762164-69762183 None:intergenic 40.0%
GGTTGACATTGTAACCTCAA+GGG + chr2_1:69762835-69762854 None:intergenic 40.0%
AGGTTGACATTGTAACCTCA+AGG + chr2_1:69762836-69762855 None:intergenic 40.0%
AGTGTAAACTCAGTGGTAAG+AGG + chr2_1:69762856-69762875 None:intergenic 40.0%
GGGTAATCTACATTGGTACA+TGG - chr2_1:69762899-69762918 Msa0221160:CDS 40.0%
AGCAAAACTAAAGCCACACT+CGG + chr2_1:69762951-69762970 None:intergenic 40.0%
TTGCTGAATAATGATGCAGG+TGG - chr2_1:69763122-69763141 Msa0221160:intron 40.0%
CATAAGCCTCTGTTCTTTCT+CGG + chr2_1:69763181-69763200 None:intergenic 40.0%
! CTGCAGTTTTCTTTTTGTCC+AGG + chr2_1:69763354-69763373 None:intergenic 40.0%
TAGGATAGAGATCATGATGC+GGG + chr2_1:69763518-69763537 None:intergenic 40.0%
!!! GAAGTCCAAGTTTTTTGAGC+AGG - chr2_1:69763574-69763593 Msa0221160:intron 40.0%
! GGTGGTGGTAACTTAATACA+TGG + chr2_1:69763647-69763666 None:intergenic 40.0%
TCCTGAAAATCAAAGCACAC+AGG - chr2_1:69763796-69763815 Msa0221160:CDS 40.0%
!! TCCTGTGTGCTTTGATTTTC+AGG + chr2_1:69763800-69763819 None:intergenic 40.0%
TTGTGGATACCTTGTTCATC+TGG - chr2_1:69763887-69763906 Msa0221160:five_prime_UTR 40.0%
AACTGAACACCAGATGAACA+AGG + chr2_1:69763899-69763918 None:intergenic 40.0%
!! GTTGGTGGTTATCTTAGTTG+CGG - chr2_1:69763956-69763975 Msa0221160:five_prime_UTR 40.0%
! TTGGTGGTTATCTTAGTTGC+GGG - chr2_1:69763957-69763976 Msa0221160:five_prime_UTR 40.0%
!! TAGTCATTTGGTGCTGATCA+AGG - chr2_1:69764065-69764084 Msa0221160:five_prime_UTR 40.0%
!! AGTCATTTGGTGCTGATCAA+GGG - chr2_1:69764066-69764085 Msa0221160:five_prime_UTR 40.0%
AGTGAGTGGAGTGATTGCTT+TGG + chr2_1:69760465-69760484 None:intergenic 45.0%
TCATAGATTGGGACAGTGAG+TGG + chr2_1:69760479-69760498 None:intergenic 45.0%
!! TGAACACCTTTGGGATCTGA+CGG + chr2_1:69760522-69760541 None:intergenic 45.0%
! CCGTCTGATTTGAACACCTT+TGG + chr2_1:69760532-69760551 None:intergenic 45.0%
! AAGGTGTTCAAATCAGACGG+TGG - chr2_1:69760532-69760551 Msa0221160:three_prime_UTR 45.0%
GGGGAAGAAATTTGACAGTG+AGG + chr2_1:69760645-69760664 None:intergenic 45.0%
CTTTCTCTGTTTCCGGATCA+TGG - chr2_1:69760736-69760755 Msa0221160:CDS 45.0%
TATCCTCCTTCTCCATGATC+CGG + chr2_1:69760751-69760770 None:intergenic 45.0%
GAATGAGTGCAGCTTCTTAC+AGG - chr2_1:69761139-69761158 Msa0221160:CDS 45.0%
TGCGTTCTTCACAAGCTTTG+TGG - chr2_1:69761480-69761499 Msa0221160:intron 45.0%
GGATGTCAACAAATGGGCAT+GGG - chr2_1:69761642-69761661 Msa0221160:intron 45.0%
TCAACAAATGGGCATGGGAA+GGG - chr2_1:69761647-69761666 Msa0221160:intron 45.0%
CTGTCAATAACAGCTTCCAC+GGG + chr2_1:69761866-69761885 None:intergenic 45.0%
GCTGTCAATAACAGCTTCCA+CGG + chr2_1:69761867-69761886 None:intergenic 45.0%
AAGCTGTTATTGACAGCAGC+AGG - chr2_1:69761870-69761889 Msa0221160:intron 45.0%
TTAGCAGGGTAAGGGTCTAA+GGG - chr2_1:69762092-69762111 Msa0221160:intron 45.0%
AAAATGCTCACGGCATTGTG+GGG + chr2_1:69762163-69762182 None:intergenic 45.0%
GAAAAATGCTCACGGCATTG+TGG + chr2_1:69762165-69762184 None:intergenic 45.0%
! CATTTTGCAACTGTCTCCGA+GGG - chr2_1:69762795-69762814 Msa0221160:intron 45.0%
! ATTTTGCAACTGTCTCCGAG+GGG - chr2_1:69762796-69762815 Msa0221160:intron 45.0%
GATTTGAACTTCGTCCCTTG+AGG - chr2_1:69762818-69762837 Msa0221160:intron 45.0%
!! TGTTGCTTTAATGCCGAGTG+TGG - chr2_1:69762935-69762954 Msa0221160:intron 45.0%
! TGCCAATAAAAGCGTGTCGA+AGG + chr2_1:69763151-69763170 None:intergenic 45.0%
! CGACACGCTTTTATTGGCAT+AGG - chr2_1:69763152-69763171 Msa0221160:intron 45.0%
AGGATTCCGAGAAAGAACAG+AGG - chr2_1:69763172-69763191 Msa0221160:intron 45.0%
AATCAACTGAGGAGCTTTGC+TGG + chr2_1:69763396-69763415 None:intergenic 45.0%
AGGATAGAGATCATGATGCG+GGG + chr2_1:69763517-69763536 None:intergenic 45.0%
TATTGTATGCAGAGAAGCGG+CGG - chr2_1:69763545-69763564 Msa0221160:intron 45.0%
TGCACCCTGCTCAAAAAACT+TGG + chr2_1:69763582-69763601 None:intergenic 45.0%
! TCTCTCACTCTTTTCTTCCG+AGG + chr2_1:69763612-69763631 None:intergenic 45.0%
TAACAGGTGAACCTGGTGAT+GGG + chr2_1:69763673-69763692 None:intergenic 45.0%
! CAACTTCAGCGCTTTTCTCA+AGG + chr2_1:69763736-69763755 None:intergenic 45.0%
!! TTTGGCGATTTTCAAGCAGC+TGG - chr2_1:69763836-69763855 Msa0221160:five_prime_UTR 45.0%
GGCGCATCTCAATAGTCATT+TGG - chr2_1:69764053-69764072 Msa0221160:five_prime_UTR 45.0%
!! GTCATTTGGTGCTGATCAAG+GGG - chr2_1:69764067-69764086 Msa0221160:five_prime_UTR 45.0%
GCTCAATGAACCAGATTCAG+TGG - chr2_1:69764123-69764142 Msa0221160:five_prime_UTR 45.0%
!!! AATTCAAATTTTTAATTTTT+AGG + chr2_1:69761030-69761049 None:intergenic 5.0%
TTCAGACCGTCAGATCCCAA+AGG - chr2_1:69760513-69760532 Msa0221160:three_prime_UTR 50.0%
CTGTTTCCGGATCATGGAGA+AGG - chr2_1:69760742-69760761 Msa0221160:CDS 50.0%
TTTCCGGATCATGGAGAAGG+AGG - chr2_1:69760745-69760764 Msa0221160:CDS 50.0%
GAAGCTGCACTCATTCTTGG+AGG + chr2_1:69761135-69761154 None:intergenic 50.0%
GGTGGTCTCTTATAGGATGG+TGG + chr2_1:69761312-69761331 None:intergenic 50.0%
GGGATGTCAACAAATGGGCA+TGG - chr2_1:69761641-69761660 Msa0221160:intron 50.0%
GTCAACAAATGGGCATGGGA+AGG - chr2_1:69761646-69761665 Msa0221160:intron 50.0%
ATAACAGCTTCCACGGGATG+TGG + chr2_1:69761860-69761879 None:intergenic 50.0%
GTTAGCAGGGTAAGGGTCTA+AGG - chr2_1:69762091-69762110 Msa0221160:intron 50.0%
! ACGGCATTGTGGGGGATTTT+TGG + chr2_1:69762154-69762173 None:intergenic 50.0%
AAATGCTCACGGCATTGTGG+GGG + chr2_1:69762162-69762181 None:intergenic 50.0%
CCTCGGAGACAGTTGCAAAA+TGG + chr2_1:69762797-69762816 None:intergenic 50.0%
! CCATTTTGCAACTGTCTCCG+AGG - chr2_1:69762794-69762813 Msa0221160:intron 50.0%
GGACGAAGTTCAAATCCCCT+CGG + chr2_1:69762814-69762833 None:intergenic 50.0%
! CGCCTTCGACACGCTTTTAT+TGG - chr2_1:69763146-69763165 Msa0221160:intron 50.0%
TATGATCGTGCAAGGCAGCT+TGG + chr2_1:69763208-69763227 None:intergenic 50.0%
! AACAGGTGAACCTGGTGATG+GGG + chr2_1:69763672-69763691 None:intergenic 50.0%
GTAACAGGTGAACCTGGTGA+TGG + chr2_1:69763674-69763693 None:intergenic 50.0%
AGTCTGTTGGAGACTTCTGC+GGG + chr2_1:69763697-69763716 None:intergenic 50.0%
GAGTCTGTTGGAGACTTCTG+CGG + chr2_1:69763698-69763717 None:intergenic 50.0%
AAGCTGCACCGAAGCGATTC+TGG + chr2_1:69760571-69760590 None:intergenic 55.0%
AAATGGAGAGCCACATCCCG+TGG - chr2_1:69761847-69761866 Msa0221160:intron 55.0%
GCAGGGTGCAAACAACTCCT+CGG - chr2_1:69763592-69763611 Msa0221160:intron 55.0%
!! ACAGGTGAACCTGGTGATGG+GGG + chr2_1:69763671-69763690 None:intergenic 55.0%
GTCTGTTGGAGACTTCTGCG+GGG + chr2_1:69763696-69763715 None:intergenic 55.0%
TGCGGGGTAACAGGTGAACC+TGG + chr2_1:69763680-69763699 None:intergenic 60.0%
GGAGACTTCTGCGGGGTAAC+AGG + chr2_1:69763689-69763708 None:intergenic 60.0%
!! TTGCGGGCTAGTAGCGTGAG+AGG - chr2_1:69763973-69763992 Msa0221160:five_prime_UTR 60.0%
!! GAACCTGGTGATGGGGGAGG+TGG + chr2_1:69763665-69763684 None:intergenic 65.0%
!! GGTGAACCTGGTGATGGGGG+AGG + chr2_1:69763668-69763687 None:intergenic 65.0%
!! CCTGGTGATGGGGGAGGTGG+TGG + chr2_1:69763662-69763681 None:intergenic 70.0%
CCACCACCTCCCCCATCACC+AGG - chr2_1:69763659-69763678 Msa0221160:intron 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 69760410 69764169 69760410 ID=Msa0221160;Name=Msa0221160
chr2_1 mRNA 69760410 69764169 69760410 ID=Msa0221160-mRNA-1;Parent=Msa0221160;Name=Msa0221160-mRNA-1;_AED=0.02;_eAED=0.02;_QI=345|1|1|1|1|1|8|307|286
chr2_1 exon 69760410 69761022 69760410 ID=Msa0221160-mRNA-1:exon:19780;Parent=Msa0221160-mRNA-1
chr2_1 exon 69761117 69761231 69761117 ID=Msa0221160-mRNA-1:exon:19779;Parent=Msa0221160-mRNA-1
chr2_1 exon 69761348 69761438 69761348 ID=Msa0221160-mRNA-1:exon:19778;Parent=Msa0221160-mRNA-1
chr2_1 exon 69762567 69762598 69762567 ID=Msa0221160-mRNA-1:exon:19777;Parent=Msa0221160-mRNA-1
chr2_1 exon 69762688 69762760 69762688 ID=Msa0221160-mRNA-1:exon:19776;Parent=Msa0221160-mRNA-1
chr2_1 exon 69762849 69762949 69762849 ID=Msa0221160-mRNA-1:exon:19775;Parent=Msa0221160-mRNA-1
chr2_1 exon 69763419 69763450 69763419 ID=Msa0221160-mRNA-1:exon:19774;Parent=Msa0221160-mRNA-1
chr2_1 exon 69763714 69764169 69763714 ID=Msa0221160-mRNA-1:exon:19773;Parent=Msa0221160-mRNA-1
chr2_1 five_prime_UTR 69763825 69764169 69763825 ID=Msa0221160-mRNA-1:five_prime_utr;Parent=Msa0221160-mRNA-1
chr2_1 CDS 69763714 69763824 69763714 ID=Msa0221160-mRNA-1:cds;Parent=Msa0221160-mRNA-1
chr2_1 CDS 69763419 69763450 69763419 ID=Msa0221160-mRNA-1:cds;Parent=Msa0221160-mRNA-1
chr2_1 CDS 69762849 69762949 69762849 ID=Msa0221160-mRNA-1:cds;Parent=Msa0221160-mRNA-1
chr2_1 CDS 69762688 69762760 69762688 ID=Msa0221160-mRNA-1:cds;Parent=Msa0221160-mRNA-1
chr2_1 CDS 69762567 69762598 69762567 ID=Msa0221160-mRNA-1:cds;Parent=Msa0221160-mRNA-1
chr2_1 CDS 69761348 69761438 69761348 ID=Msa0221160-mRNA-1:cds;Parent=Msa0221160-mRNA-1
chr2_1 CDS 69761117 69761231 69761117 ID=Msa0221160-mRNA-1:cds;Parent=Msa0221160-mRNA-1
chr2_1 CDS 69760717 69761022 69760717 ID=Msa0221160-mRNA-1:cds;Parent=Msa0221160-mRNA-1
chr2_1 three_prime_UTR 69760410 69760716 69760410 ID=Msa0221160-mRNA-1:three_prime_utr;Parent=Msa0221160-mRNA-1
Gene Sequence

>Msa0221160

ATGGAGAAGGAGGATAACAAACCCGACATTCAGAAAATTGGGAACGAAATTGAAGATACTGAACCCATAGAACTCGTTCTTTTTCAAGTTCCCGAGTGTTACGTCTACATAATACCTCCAAGAATGAGTGCAGCTTCTTACAGGGCTGATGAATGGGATGTCAACAAATGGGCATGGGAAGGGATATTGAAAGTTGTTAGCAAGGGAGAAGAATGTATCATAAAACTAGAAGATAAGAACACAGGTGAATTATATGCTCGAGCATTTTTAAGAAATGGAGAGCCACATCCCGTGGAAGCTGTTATTGACAGCAGCAGATATTTTGTTCTTCGCATAGAAGAAAACATAGGTGGTCGCCTTCGACACGCTTTTATTGGCATAGGATTCCGAGAAAGAACAGAGGCTTATGACTTCCAAGCTGCCTTGCACGATCATATGAAATACCTGGACAAAAAGAAAACTGCAGAAGAGATGGAACAACATTACCAGCAAAGCTCCTCAGTTGATTACAGTTTGAAAGAAGGGGAGACTCTTGTGCTGCAAATCAAGAACAATAGAAGCGGCGGCAATGTGAAGTCCAAGTTTTTTGAGCAGGGTGCAAACAACTCCTCGGAAGAAAAGAGTGAGAGAAAAGAATCTATACCATGTATTAAGTTACCACCACCTCCCCCATCACCAGGTTCACCTGTTACCCCGCAGAAGTCTCCAACAGACTCACCTACAAAATTAAGCCTTGAGAAAAGCGCTGAAGTTGAGACCTCTAAAATAGTTAAAGAAACAGAACATGAAAAATCTCCTGAAAATCAAAGCACACAGGATGTAGTAGATGATGATTTTGGCGATTTTCAAGCAGCTGGTTAA
Protein sequence

>Msa0221160

MEKEDNKPDIQKIGNEIEDTEPIELVLFQVPECYVYIIPPRMSAASYRADEWDVNKWAWEGILKVVSKGEECIIKLEDKNTGELYARAFLRNGEPHPVEAVIDSSRYFVLRIEENIGGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLDKKKTAEEMEQHYQQSSSVDYSLKEGETLVLQIKNNRSGGNVKSKFFEQGANNSSEEKSERKESIPCIKLPPPPPSPGSPVTPQKSPTDSPTKLSLEKSAEVETSKIVKETEHEKSPENQSTQDVVDDDFGDFQAAG*