AlfalfaGEDB | Msa0300970

M. sativa cultivar zhongmu-4 / Msa0300970

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300970	XP_003597191.2	93.951	810	48	1	1	809	1	810	0.0	1544

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300970	sp\|Q9ZTX9\|ARFD_ARATH	58.453	763	273	12	54	809	63	788	0.0	825

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300970	Q2HSV9	93.951	810	48	1	1	809	1	810	0.0	1544

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TFs/TRs:

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Gene ID	Type	Classification
Msa0300970	TF	B3-ARF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0300970	Msa0338510	0.875989	1.993281e-68	-8.615850e-47
Msa0300970	Msa1347520	0.929835	3.927075e-93	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300970	MtrunA17_Chr2g0326281	92.467	823	48	2	1	809	1	823	0.0	1533
Msa0300970	MtrunA17_Chr4g0029671	73.016	819	184	12	1	809	1	792	0.0	1137
Msa0300970	MtrunA17_Chr4g0046371	42.587	634	325	13	51	666	33	645	1.61e-148	452
Msa0300970	MtrunA17_Chr2g0282961	55.037	407	160	8	55	454	52	442	3.56e-141	430
Msa0300970	MtrunA17_Chr8g0385791	47.198	464	225	8	14	472	21	469	8.92e-136	423
Msa0300970	MtrunA17_Chr8g0385791	49.038	104	53	0	676	779	705	808	3.86e-29	124
Msa0300970	MtrunA17_Chr2g0276781	51.169	385	169	5	36	418	7	374	1.90e-126	394
Msa0300970	MtrunA17_Chr2g0276781	46.154	130	66	2	673	798	539	668	6.90e-27	117
Msa0300970	MtrunA17_Chr5g0433301	48.889	405	172	10	56	452	24	401	2.61e-112	362
Msa0300970	MtrunA17_Chr5g0433301	35.971	139	81	4	634	769	670	803	5.18e-18	89.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0300970	AT5G60450.2	58.409	767	275	12	50	809	35	764	0.0	827
Msa0300970	AT5G60450.1	58.453	763	273	12	54	809	63	788	0.0	825
Msa0300970	AT5G62000.2	36.501	863	440	21	14	806	29	853	9.26e-171	515
Msa0300970	AT5G62000.5	36.501	863	440	21	14	806	29	853	9.26e-171	515
Msa0300970	AT5G62000.3	36.501	863	440	21	14	806	29	853	9.26e-171	515
Msa0300970	AT5G62000.1	36.501	863	440	21	14	806	29	853	9.26e-171	515
Msa0300970	AT5G62000.4	37.260	832	419	20	14	775	29	827	1.65e-170	514
Msa0300970	AT2G33860.1	49.896	481	192	10	55	529	53	490	1.96e-147	446
Msa0300970	AT3G61830.1	47.222	396	186	8	43	430	12	392	2.09e-117	368
Msa0300970	AT3G61830.1	48.039	102	52	1	672	773	480	580	3.27e-23	105

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Find 199 sgRNAs with CRISPR-Local

Find 339 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTATGCTAAAAGCATC+AGG	0.463277	2_3:-56650338	Msa0300970:CDS
AAAATGACATTATGGTTGTT+GGG	0.432406	2_3:-56648642	Msa0300970:CDS
AAACCGCGAGAAAATTGTGA+GGG	0.682065	2_3:-56652439	Msa0300970:five_prime_UTR
AAACTTAACTTTCACTCATT+AGG	0.322689	2_3:-56649279	Msa0300970:CDS
AAATGACATTATGGTTGTTG+GGG	0.611574	2_3:-56648641	Msa0300970:CDS
AAATTATTGACACATTCATT+TGG	0.344864	2_3:+56647616	None:intergenic
AAATTTCGCCATATTTATCG+AGG	0.663380	2_3:-56650950	Msa0300970:intron
AACATTCTCTAGTTGGAAAT+TGG	0.212031	2_3:+56649170	None:intergenic
AACCTCATCATTCTCCTTAT+TGG	0.160497	2_3:+56651861	None:intergenic
AACTAGATTCTAACTACTGT+TGG	0.511297	2_3:+56648029	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAACTAGACTAATTTATCCT+CGG	-	chr2_3:56647686-56647705	Msa0300970:intron	30.0%
AACCAACCAAAAAACATTGT+TGG	-	chr2_3:56647811-56647830	Msa0300970:intron	30.0%
CTTCATCTTATCTTGAACTA+TGG	-	chr2_3:56647990-56648009	Msa0300970:three_prime_UTR	30.0%
TTAACATCACTTCCAAAGAA+AGG	-	chr2_3:56648031-56648050	Msa0300970:CDS	30.0%
TAACATCACTTCCAAAGAAA+GGG	-	chr2_3:56648032-56648051	Msa0300970:CDS	30.0%
TCATTGGAAATTCCAACTTA+TGG	-	chr2_3:56648118-56648137	Msa0300970:CDS	30.0%
TGATACACATCATTGATGAA+TGG	-	chr2_3:56648523-56648542	Msa0300970:intron	30.0%
AGATTCATAGTTACCTTATG+AGG	+	chr2_3:56648708-56648727	None:intergenic	30.0%
AAATTTCGCCATATTTATCG+AGG	-	chr2_3:56649217-56649236	Msa0300970:CDS	30.0%
ACAGAAATATTGTGATTGGT+TGG	-	chr2_3:56649248-56649267	Msa0300970:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	56647605	56652584	56647605	ID=Msa0300970;Name=Msa0300970
chr2_3	mRNA	56647605	56652584	56647605	ID=Msa0300970-mRNA-1;Parent=Msa0300970;Name=Msa0300970-mRNA-1;_AED=0.16;_eAED=0.16;_QI=234\|1\|1\|1\|0.91\|0.84\|13\|214\|809
chr2_3	exon	56647605	56647666	56647605	ID=Msa0300970-mRNA-1:exon:18210;Parent=Msa0300970-mRNA-1
chr2_3	exon	56647879	56648214	56647879	ID=Msa0300970-mRNA-1:exon:18209;Parent=Msa0300970-mRNA-1
chr2_3	exon	56648628	56648818	56648628	ID=Msa0300970-mRNA-1:exon:18208;Parent=Msa0300970-mRNA-1
chr2_3	exon	56648915	56649675	56648915	ID=Msa0300970-mRNA-1:exon:18207;Parent=Msa0300970-mRNA-1
chr2_3	exon	56649758	56649920	56649758	ID=Msa0300970-mRNA-1:exon:18206;Parent=Msa0300970-mRNA-1
chr2_3	exon	56650117	56650195	56650117	ID=Msa0300970-mRNA-1:exon:18205;Parent=Msa0300970-mRNA-1
chr2_3	exon	56650273	56650392	56650273	ID=Msa0300970-mRNA-1:exon:18204;Parent=Msa0300970-mRNA-1
chr2_3	exon	56650497	56650661	56650497	ID=Msa0300970-mRNA-1:exon:18203;Parent=Msa0300970-mRNA-1
chr2_3	exon	56650774	56650864	56650774	ID=Msa0300970-mRNA-1:exon:18202;Parent=Msa0300970-mRNA-1
chr2_3	exon	56650951	56651035	56650951	ID=Msa0300970-mRNA-1:exon:18201;Parent=Msa0300970-mRNA-1
chr2_3	exon	56651262	56651447	56651262	ID=Msa0300970-mRNA-1:exon:18200;Parent=Msa0300970-mRNA-1
chr2_3	exon	56651828	56651884	56651828	ID=Msa0300970-mRNA-1:exon:18199;Parent=Msa0300970-mRNA-1
chr2_3	exon	56652003	56652584	56652003	ID=Msa0300970-mRNA-1:exon:18198;Parent=Msa0300970-mRNA-1
chr2_3	five_prime_UTR	56652351	56652584	56652351	ID=Msa0300970-mRNA-1:five_prime_utr;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56652003	56652350	56652003	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56651828	56651884	56651828	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56651262	56651447	56651262	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56650951	56651035	56650951	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56650774	56650864	56650774	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56650497	56650661	56650497	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56650273	56650392	56650273	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56650117	56650195	56650117	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56649758	56649920	56649758	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56648915	56649675	56648915	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56648628	56648818	56648628	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	CDS	56648031	56648214	56648031	ID=Msa0300970-mRNA-1:cds;Parent=Msa0300970-mRNA-1
chr2_3	three_prime_UTR	56647879	56648030	56647879	ID=Msa0300970-mRNA-1:three_prime_utr;Parent=Msa0300970-mRNA-1
chr2_3	three_prime_UTR	56647605	56647666	56647605	ID=Msa0300970-mRNA-1:three_prime_utr;Parent=Msa0300970-mRNA-1

Gene Sequence

>Msa0300970

ATGGATATTGATCTGAATGATTCCACAACACAAGTTAAGAAGAATATATGTTGCAATGGTGAATGTGAGAAAAGTGTTTGTTGTGTTTGCACTTTATCTTCTTCTTCATCACCAACATGTTCATCTTCTAGTTCCACTTCGGCTCTTGTTTCTTCATCTTATCTTGAACTATGGCATTCTTGTGCTGGACCTTTAACATCACTTCCAAAGAAAGGGAATGTTGTAGTTTATTTTCCACAAGGTCATTTGGAACAAGTTGCATCTTTGTCTCTCTTTTCTTCATTGGAAATTCCAACTTATGGTCTTCAACCACAAATCTTTTGTAGGGTTGTCAATGTTCAATTACTTGCCAATAAGGAGAATGATGAGGTTTACACACAGGTTACTTTGCTTCCACAGGCAGAGTTAGCAGGGATGTGTTTGGATGACAAAGAGCCTGAAGGTTTAGAAGCAGGTGGAGAGGGAGATGGAAGATCGCCTACAAAGTTAGCTTCTCACATGTTTTGCAAAACACTCACTGCCTCTGATACCAGCACTCATGGAGGGTTTTCTGTTCCTCGTCGAGCTGCTGAAGATTGTTTTCCTCCTTTGGATTATAAGCAGCAGAGACCCTCTCAAGAGCTTGTTGCAAAAGACCTGCATGGTGTGGAGTGGAAATTTCGCCATATTTATCGAGGTCAGCCAAGGAGGCATCTGCTCACTACTGGCTGGAGTGCTTTTGTTAGCCAAAAGAATCTTGTTTCTGGGGACGCAGTGCTCTTTCTAAGGGGTGAAAACGGAGAGTTAAGATTGGGAATCAGAAGAGCTGCTCGACCGAGAAACGGTCTTCCTGAATCAATTGTTGGTAACCAGAGTTGTTCTCCTAGTTTCCTTTCTTCAGTGGCTAATGCTATCTCCGCAAAAAGCATGTTTCATGTTTTCTACAGTCCAAGAGCTAGTCATGCCGATTTCGTTGTGCCTTACCAAAAGTATGCTAAAAGCATCAGGAACCCACTGACCATAGGGACAAGATTCAAAATGAAATTTGAAATGGATGAATCTCCAGAAAGAAGGTGTAGTAGCGGTATAGTGACTGGAATGAGTGATTTGGATCCCTATAAATGGCCCAAGTCAAAATGGAGATGCTTGATGGTCCGATGGGATGAGGATATTGAGGCTAATCATCAAGATAGAGTCTCTCCTTGGGAGATCGATCCGTCTACTTCTCTCCCTCCCTTGAACATTCAATCTTGTCGAAGGCTGAAGAAACTGCGGACGGGTCTGCAAGTTGAATCACCCAGTCACCTTATCACTGCTGGAGACAGTGGGTTTATGGACTTTGATGAGTCTATAAGATCGACCAAGGTCTTGCAAGGTCAAGAAAAAACAAGTTTTATGTCACTATATTATGGATGTGACACAGTAACTAAGCAGAAAGAATTTGATATCAATTCTCTAAGACATACTAATCTTGCATCAAATGGAGCAAGAAAGATTACTTCTTCCGAGTTTACGAGGGTTCAGCCTTCTAGTTATGCAGACTTCACGGAAACGAACCGGTTTCCTAGGGTCTTGCAAGGTCAAGAAATATATCCATTGAGATCCCTGGCAGGAAAGGTTGATCTAAACCTTAACTCTTGGGGTAAAACCAATGTTAGTTACACAAATTATAACCTGCATAATGCAACCAAACTTAACTTTCACTCATTAGGATCAGAAGTTCTTCAAAATTCCTCTTTTCCATATGGTGATATACACAAAGTTGGTCAAGGTAGCAGCATGCTGTGCTCCAAACCAACCAATTTCCAACTAGAGAATGTTTCATTTAATACTCCTTCAGCTCAGATAGGTGCATTGAGAAATGAAGTTGGAATATCAGGTTTCACAATCCGAAACAAGCAGAAGCTGCAGGACAATATTTCTGCTGCTGCTTCTCTAGGTGCAAACTTGAGGATTTCAAATGACAAAAATTTCAACGCGAAGGTAAATCCTTGTAGACTATTTGGATTTTCCTTATCGGCGGCGGAAACCACCTCACAAAATCTACAGAACTCTGCTAAAAGAAGTTGCACGAAGGTTCACAAACAAAGGAGCTTAGTTGGAAGAGCCATTGATCTCTCAAGATTGAGCAGCTACAATGATCTGGTGAGTGAACTAGAGAGACTATTTGGCATGGAAGGTCTTCTAAGAGATCCTGACAAAGGATGGAGAATCCTCTACACTGACAGTGAAAATGACATTATGGTTGTTGGGGATGATCCATGGCATGAGTTCTGTGTTATGGTATCAAAAATCCATATATATACACAAGAAGAAGTGGAGAAGATGACAATTGGGATGATGAATGATGATAACCAGAGTTGTTTGGAACATACACCATTGATTGTGGAGGCTTCAAAGTCTTCCTCAGTGGGACAGCCAGATTCTTCTCCAACAGTAGTTAGAATCTAG

Protein sequence

>Msa0300970

MDIDLNDSTTQVKKNICCNGECEKSVCCVCTLSSSSSPTCSSSSSTSALVSSSYLELWHSCAGPLTSLPKKGNVVVYFPQGHLEQVASLSLFSSLEIPTYGLQPQIFCRVVNVQLLANKENDEVYTQVTLLPQAELAGMCLDDKEPEGLEAGGEGDGRSPTKLASHMFCKTLTASDTSTHGGFSVPRRAAEDCFPPLDYKQQRPSQELVAKDLHGVEWKFRHIYRGQPRRHLLTTGWSAFVSQKNLVSGDAVLFLRGENGELRLGIRRAARPRNGLPESIVGNQSCSPSFLSSVANAISAKSMFHVFYSPRASHADFVVPYQKYAKSIRNPLTIGTRFKMKFEMDESPERRCSSGIVTGMSDLDPYKWPKSKWRCLMVRWDEDIEANHQDRVSPWEIDPSTSLPPLNIQSCRRLKKLRTGLQVESPSHLITAGDSGFMDFDESIRSTKVLQGQEKTSFMSLYYGCDTVTKQKEFDINSLRHTNLASNGARKITSSEFTRVQPSSYADFTETNRFPRVLQGQEIYPLRSLAGKVDLNLNSWGKTNVSYTNYNLHNATKLNFHSLGSEVLQNSSFPYGDIHKVGQGSSMLCSKPTNFQLENVSFNTPSAQIGALRNEVGISGFTIRNKQKLQDNISAAASLGANLRISNDKNFNAKVNPCRLFGFSLSAAETTSQNLQNSAKRSCTKVHKQRSLVGRAIDLSRLSSYNDLVSELERLFGMEGLLRDPDKGWRILYTDSENDIMVVGDDPWHEFCVMVSKIHIYTQEEVEKMTIGMMNDDNQSCLEHTPLIVEASKSSSVGQPDSSPTVVRI*