AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0303130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0303130 MtrunA17_Chr2g0329651 91.629 657 11 6 62 677 1 654 0.0 1224
Msa0303130 MtrunA17_Chr2g0319591 50.581 172 84 1 390 560 170 341 2.28e-51 184
Msa0303130 MtrunA17_Chr4g0041821 44.500 200 108 2 368 564 144 343 1.35e-48 176
Msa0303130 MtrunA17_Chr1g0183581 46.012 163 87 1 390 551 153 315 1.07e-44 165
Msa0303130 MtrunA17_Chr3g0127461 45.902 122 65 1 426 547 340 460 7.37e-32 130
Msa0303130 MtrunA17_Chr1g0146661 45.902 122 65 1 426 547 385 505 1.38e-31 130
Msa0303130 MtrunA17_Chr4g0064141 40.141 142 82 2 408 547 434 574 2.15e-26 114
Msa0303130 MtrunA17_Chr2g0329691 25.909 220 119 5 391 571 202 416 4.85e-14 75.1
Msa0303130 MtrunA17_Chr4g0033831 25.837 209 115 4 402 571 216 423 1.05e-13 73.9
Msa0303130 MtrunA17_Chr3g0121451 27.273 165 97 5 402 545 209 371 4.30e-12 68.9
Msa0303130 MtrunA17_Chr8g0378291 29.560 159 89 5 408 545 226 382 6.31e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0303130 AT2G43490.6 50.815 675 245 15 64 677 2 650 0.0 610
Msa0303130 AT2G43490.8 50.815 675 245 15 64 677 2 650 0.0 610
Msa0303130 AT2G43490.5 50.663 679 244 16 64 677 2 654 0.0 605
Msa0303130 AT2G43490.4 50.663 679 244 16 64 677 2 654 0.0 605
Msa0303130 AT2G43490.1 50.663 679 244 16 64 677 2 654 0.0 605
Msa0303130 AT2G43490.7 50.663 679 244 16 64 677 2 654 0.0 605
Msa0303130 AT2G43490.3 50.663 679 244 16 64 677 2 654 0.0 605
Msa0303130 AT2G43490.2 50.663 679 244 16 64 677 2 654 0.0 605
Msa0303130 AT3G59570.2 50.742 674 253 13 62 677 1 653 0.0 599
Msa0303130 AT3G59570.3 50.742 674 253 13 62 677 1 653 0.0 599
Msa0303130 AT3G59570.1 49.848 660 256 13 62 677 1 629 0.0 570
Msa0303130 AT4G27100.2 52.174 161 76 1 392 551 163 323 2.17e-50 182
Msa0303130 AT4G27100.1 52.174 161 76 1 392 551 163 323 3.71e-50 181
Msa0303130 AT5G54780.1 43.256 215 107 2 392 605 161 361 4.42e-50 181
Msa0303130 AT2G20440.4 42.051 195 108 2 371 560 16 210 4.59e-48 172
Msa0303130 AT2G20440.1 42.051 195 108 2 371 560 132 326 1.15e-46 171
Msa0303130 AT2G20440.2 42.051 195 108 2 371 560 132 326 1.15e-46 171
Msa0303130 AT4G28550.1 41.951 205 114 2 352 551 113 317 2.14e-46 170
Msa0303130 AT4G28550.3 41.872 203 113 2 352 549 113 315 1.63e-43 160
Msa0303130 AT2G20440.3 40.201 199 114 2 352 545 113 311 5.54e-43 159
Msa0303130 AT4G28550.2 41.919 198 110 2 352 544 113 310 7.98e-43 158
Msa0303130 AT5G41940.1 45.902 122 65 1 426 547 345 465 5.78e-31 128
Msa0303130 AT3G49350.1 42.537 134 75 2 414 547 319 450 8.51e-31 127
Msa0303130 AT5G53570.1 45.082 122 66 1 426 547 338 458 2.24e-30 126
Msa0303130 AT5G24390.1 43.443 122 68 1 426 547 317 437 3.37e-30 125
Msa0303130 AT5G53570.2 45.082 122 66 1 426 547 365 485 3.39e-30 126
Msa0303130 AT5G52580.1 35.429 175 106 3 379 547 409 582 1.14e-25 112
Msa0303130 AT5G52580.2 35.429 175 106 3 379 547 426 599 1.25e-25 112
Msa0303130 AT5G52580.3 34.615 182 112 3 372 547 408 588 1.40e-25 112
Msa0303130 AT2G30710.1 26.794 209 113 4 402 571 201 408 2.71e-14 76.3
Msa0303130 AT4G13730.2 28.916 166 95 5 401 545 227 390 8.87e-14 74.3
Msa0303130 AT4G13730.1 28.571 168 97 5 401 547 227 392 1.34e-13 73.9
Msa0303130 AT4G13730.3 28.916 166 95 5 401 545 216 379 1.47e-13 73.9

Find 170 sgRNAs with CRISPR-Local

Find 9 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCTTGTCAATCTTCTTAT+TGG 0.094457 2_3:+60270306 None:intergenic
TGCTATTACTACATCAATTT+TGG 0.143138 2_3:-60270159 Msa0303130:three_prime_UTR
TCTGGTTCTCTCCCTATCTT+TGG 0.215426 2_3:+60276492 None:intergenic
ACCATTGCAGGGAGCTTTAT+AGG 0.223747 2_3:-60276839 Msa0303130:intron
TGGTTTCTCGAACGATCTTC+TGG 0.254616 2_3:+60274053 None:intergenic
AGGATAACGCAGATGCATTT+TGG 0.267217 2_3:-60275676 Msa0303130:CDS
TACAACACAACAACCTTTAC+AGG 0.281282 2_3:+60280012 None:intergenic
CATCGATTAGACCCGAAGTT+TGG 0.294190 2_3:-60277699 Msa0303130:CDS
ACCATAGGCGTTTCATAAAC+TGG 0.303016 2_3:+60276177 None:intergenic
CTGGTTCTCTCCCTATCTTT+GGG 0.312840 2_3:+60276493 None:intergenic
GTCCGACAAATTGGCGGTCT+TGG 0.313584 2_3:-60270256 Msa0303130:three_prime_UTR
TCCCTTTGTTGTAATCTTTG+AGG 0.319573 2_3:-60275696 Msa0303130:CDS
TGCTCCCATGTCCGACAAAT+TGG 0.332509 2_3:-60270265 Msa0303130:three_prime_UTR
ATCGAGGCACTTGCCATTGC+TGG 0.333275 2_3:+60276123 None:intergenic
AGTCTATGTTGTTGTTGATA+TGG 0.339014 2_3:+60276307 None:intergenic
CACATGATGCGGCTGCGGGC+TGG 0.350015 2_3:+60274261 None:intergenic
GTATGTTGCTAGTTCTCTTT+CGG 0.354407 2_3:-60275408 Msa0303130:CDS
GTGGCTTGGCAAGGAATATC+TGG 0.354583 2_3:-60274180 Msa0303130:CDS
AACCACAAAGATTAACTCTA+TGG 0.358752 2_3:+60270052 None:intergenic
GGTTCATTGGCATATATTGT+TGG 0.362265 2_3:-60276771 Msa0303130:CDS
TGTTGTAGTATACGCCTATA+TGG 0.363415 2_3:-60270077 Msa0303130:three_prime_UTR
CCATTGCAGGGAGCTTTATA+GGG 0.367979 2_3:-60276838 Msa0303130:intron
CAGCTGACTTTGATGAATCC+TGG 0.371016 2_3:-60274438 Msa0303130:CDS
ATCCTGGGCATTTGAGTTAG+AGG 0.375355 2_3:-60274422 Msa0303130:CDS
AGCTGCTTCATCACCCTAGT+TGG 0.375614 2_3:+60275523 None:intergenic
AAGAAATGGAGGGTAGATAC+AGG 0.376744 2_3:-60270109 Msa0303130:three_prime_UTR
GGATATATATTATAATATAA+TGG 0.377225 2_3:-60270008 Msa0303130:three_prime_UTR
TATTGTTGGCTCCAAAGTTA+TGG 0.384097 2_3:-60276757 Msa0303130:CDS
CTTCTTCGATCCTTGCGTTT+CGG 0.385986 2_3:-60280391 Msa0303130:intron
ATCGATTAGACCCGAAGTTT+GGG 0.386184 2_3:-60277698 Msa0303130:CDS
ATAGAATTCAAGATGATTAT+CGG 0.389043 2_3:+60275927 None:intergenic
AACTGCAGTTCCCCAAAGAT+AGG 0.389686 2_3:-60276504 Msa0303130:CDS
GTTTCAATTTCAGAGTTTAC+AGG 0.394565 2_3:+60276243 None:intergenic
CACATGCATAGTAGAAGAAA+TGG 0.396870 2_3:-60270123 Msa0303130:three_prime_UTR
AACCAGATGGATCACATAAT+AGG 0.398593 2_3:-60276476 Msa0303130:CDS
TACCATAGAGTTAATCTTTG+TGG 0.404549 2_3:-60270054 Msa0303130:three_prime_UTR
ATAGAGTATCAGAATGGCTT+TGG 0.406487 2_3:-60276074 Msa0303130:CDS
TGAAGCAGCTGCGAGCATTA+TGG 0.407350 2_3:-60275510 Msa0303130:CDS
TCTTTGTGGTTGATTTGAAT+TGG 0.408449 2_3:-60270040 Msa0303130:three_prime_UTR
ATCATGAAAGGTCTGATGAT+TGG 0.410469 2_3:-60276599 Msa0303130:CDS
AATTGGCGGTCTTGGATGCT+TGG 0.423623 2_3:-60270248 Msa0303130:three_prime_UTR
TGAAAGTGGTGAATGTGAAT+TGG 0.425091 2_3:+60280420 None:intergenic
CCTTTCTCACTCTCCTGTAA+AGG 0.427883 2_3:-60280025 Msa0303130:intron
GGATATGAGGACCTCCTCTA+AGG 0.430223 2_3:-60276736 Msa0303130:CDS
AACGAGAACTATTTCCTCCT+TGG 0.430909 2_3:-60274137 Msa0303130:CDS
GACTTAGTTATTGAATCAAA+TGG 0.431905 2_3:-60276288 Msa0303130:CDS
GTGTTCACATGATGCGGCTG+CGG 0.436166 2_3:+60274256 None:intergenic
GAAGTTTGGGAGTTCTTACT+AGG 0.436180 2_3:-60277685 Msa0303130:CDS
AGGAATATCTGGTCAAGAAA+TGG 0.436570 2_3:-60274169 Msa0303130:CDS
AAGTGTGGGGACTGGTTCAT+TGG 0.438865 2_3:-60276784 Msa0303130:CDS
AGTAAGAACTCCCAAACTTC+GGG 0.439071 2_3:+60277688 None:intergenic
TGCCAAGCCACAAAAGGTAT+TGG 0.441452 2_3:+60274192 None:intergenic
AGTTTGTGATGTATTGTAAC+GGG 0.445875 2_3:-60280149 Msa0303130:CDS
ATCGTTTCCTTTCCTTGCCA+AGG 0.446738 2_3:+60274120 None:intergenic
AGCTGACTTTGATGAATCCT+GGG 0.450356 2_3:-60274437 Msa0303130:CDS
CAAGGTTGAGAGCAGCTAGA+AGG 0.454731 2_3:-60277627 Msa0303130:intron
GAATGCTTAAGCCGGACATA+TGG 0.455185 2_3:-60279019 Msa0303130:CDS
ATCCAAGACCGCCAATTTGT+CGG 0.456245 2_3:+60270254 None:intergenic
TTTACAGGATGGGAGGTAGT+AGG 0.456297 2_3:+60276258 None:intergenic
TGCATGGGTTGATCCTGCTA+CGG 0.456884 2_3:-60275857 Msa0303130:CDS
AACAGATTGGTGACAGGTAA+AGG 0.460981 2_3:+60276429 None:intergenic
GCTGCTTCATCACCCTAGTT+GGG 0.463057 2_3:+60275524 None:intergenic
TCCTCAAAGATTACAACAAA+GGG 0.467132 2_3:+60275695 None:intergenic
TGAGTTAGAGGAGAATTGTC+TGG 0.473125 2_3:-60274410 Msa0303130:CDS
GGATTGTCAGATGGAAAGTC+TGG 0.473418 2_3:+60276198 None:intergenic
GCATGGGTTGATCCTGCTAC+GGG 0.473581 2_3:-60275856 Msa0303130:CDS
ATCCTATTATGTGATCCATC+TGG 0.478198 2_3:+60276474 None:intergenic
GACCGCCAATTTGTCGGACA+TGG 0.478494 2_3:+60270260 None:intergenic
TCATGAAAGGTCTGATGATT+GGG 0.478932 2_3:-60276598 Msa0303130:CDS
GGTCCTCATATCCATAACTT+TGG 0.486021 2_3:+60276746 None:intergenic
AGAAATGGAGGGTAGATACA+GGG 0.488943 2_3:-60270108 Msa0303130:three_prime_UTR
GATTTGAATTGGCAAAAGAA+AGG 0.489069 2_3:-60270029 Msa0303130:three_prime_UTR
AAGTCTGGTTCATCTTCATC+AGG 0.495295 2_3:+60276213 None:intergenic
TATAAGCTGAACAAAATTGA+AGG 0.495603 2_3:+60270204 None:intergenic
CAGGGAGCTTTATAGGGACC+TGG 0.497386 2_3:-60276832 Msa0303130:CDS
GACAAGGCTTGCCATATGTC+CGG 0.499718 2_3:+60279008 None:intergenic
ATATCCTTGCTGTCTATGCA+TGG 0.500200 2_3:-60275873 Msa0303130:CDS
GGAGAATTGTCTGGAAGCAT+TGG 0.500684 2_3:-60274401 Msa0303130:CDS
CAATGGAGTTGTGGAAAAGC+TGG 0.501641 2_3:-60280102 Msa0303130:CDS
TGTTCACATGATGCGGCTGC+GGG 0.504103 2_3:+60274257 None:intergenic
TTAACTCTATGGTACCATAT+AGG 0.505395 2_3:+60270063 None:intergenic
TAGTAAGAACTCCCAAACTT+CGG 0.506469 2_3:+60277687 None:intergenic
CCTTTACAGGAGAGTGAGAA+AGG 0.506711 2_3:+60280025 None:intergenic
ATCTTGAATTCTATGAGGAC+AGG 0.507239 2_3:-60275918 Msa0303130:CDS
GCGGTCTTGGATGCTTGGAC+TGG 0.510361 2_3:-60270243 Msa0303130:three_prime_UTR
TCGAACTAACTAGGTTAAGT+TGG 0.512679 2_3:+60276571 None:intergenic
GTGCCTCGATGTCTCAAGGA+TGG 0.513704 2_3:-60276110 Msa0303130:CDS
ATATAATTGCTTGAGTCTCT+AGG 0.516182 2_3:+60274367 None:intergenic
CTCCTCTAACTCAAATGCCC+AGG 0.520118 2_3:+60274420 None:intergenic
CGAAGAAGAATGATTGAAAG+TGG 0.521090 2_3:+60280406 None:intergenic
TAATGAGGCTCTTTCAATGT+GGG 0.521668 2_3:-60275371 Msa0303130:CDS
AATGGCTTTGGACTTTGCAC+AGG 0.522971 2_3:-60276062 Msa0303130:CDS
AATTTCAGAGTTTACAGGAT+GGG 0.523022 2_3:+60276248 None:intergenic
ATTTCCTCCTTGGCAAGGAA+AGG 0.523765 2_3:-60274127 Msa0303130:CDS
GATCTTACAAATGACAAAAG+AGG 0.525460 2_3:-60269945 Msa0303130:three_prime_UTR
TTGCTTGACATTGCTTAACC+AGG 0.528082 2_3:+60276814 None:intergenic
TCCCATGTCCGACAAATTGG+CGG 0.528861 2_3:-60270262 Msa0303130:three_prime_UTR
ATTCCTTGCCAAGCCACAAA+AGG 0.531215 2_3:+60274186 None:intergenic
CCTTGGCAATACCCCGTAGC+AGG 0.533513 2_3:+60275844 None:intergenic
TTCTGATACTCTATCTTCAC+TGG 0.533705 2_3:+60276083 None:intergenic
CTTTGGACTTTGCACAGGAT+AGG 0.535773 2_3:-60276057 Msa0303130:intron
CAAGCAATGCATTCAAGTGT+GGG 0.538780 2_3:-60276798 Msa0303130:CDS
TTCTAGTTGTTGATGTTGTG+AGG 0.541560 2_3:-60275951 Msa0303130:intron
GAAATGGAGGGTAGATACAG+GGG 0.543297 2_3:-60270107 Msa0303130:three_prime_UTR
TGGTTCTCTCCCTATCTTTG+GGG 0.544821 2_3:+60276494 None:intergenic
TCCAGTTTATGAAACGCCTA+TGG 0.546219 2_3:-60276178 Msa0303130:intron
ATCCTCAAAGATTACAACAA+AGG 0.549774 2_3:+60275694 None:intergenic
TTAAAATGGAAGGCCCAACT+AGG 0.550468 2_3:-60275537 Msa0303130:CDS
AATATCAAGCAATGGAGTTG+TGG 0.554855 2_3:-60280111 Msa0303130:CDS
ACTGCAGTTCCCCAAAGATA+GGG 0.557828 2_3:-60276503 Msa0303130:CDS
TAATCATCTTGAATTCTATG+AGG 0.560760 2_3:-60275923 Msa0303130:CDS
TCATAAACTGGATTGTCAGA+TGG 0.561549 2_3:+60276189 None:intergenic
ATGCATTCAAGTGTGGGGAC+TGG 0.564693 2_3:-60276792 Msa0303130:CDS
TGCCTCGATGTCTCAAGGAT+GGG 0.565324 2_3:-60276109 Msa0303130:CDS
TCAAGCAATGCATTCAAGTG+TGG 0.565429 2_3:-60276799 Msa0303130:CDS
GCTCCAAAGTTATGGATATG+AGG 0.568008 2_3:-60276749 Msa0303130:CDS
GCCAATAAGAAGATTGACAA+GGG 0.568797 2_3:-60270307 Msa0303130:exon
TACTTGCTCTCGAACTAACT+AGG 0.569309 2_3:+60276562 None:intergenic
AAATTGAAGCTAAACTAGAA+AGG 0.569768 2_3:-60276704 Msa0303130:CDS
CTTTCCTTCACTATCAGACA+AGG 0.571143 2_3:+60278992 None:intergenic
AATGGCTATTGGAAGAAGCA+TGG 0.573626 2_3:-60280509 Msa0303130:CDS
TAAAATGGAAGGCCCAACTA+GGG 0.576107 2_3:-60275536 Msa0303130:CDS
CAAACTTCGGGTCTAATCGA+TGG 0.577058 2_3:+60277700 None:intergenic
ACATACGAAATGCAAAATGA+AGG 0.577837 2_3:+60275425 None:intergenic
CTTGTCTGATAGTGAAGGAA+AGG 0.581526 2_3:-60278991 Msa0303130:intron
GCAAGTGCCTCGATGTCTCA+AGG 0.582191 2_3:-60276114 Msa0303130:CDS
ACAGGATGGGAGGTAGTAGG+AGG 0.582418 2_3:+60276261 None:intergenic
GAGTTTGTGATGTATTGTAA+CGG 0.590011 2_3:-60280150 Msa0303130:CDS
TGAGGCTCTTTCAATGTGGG+AGG 0.594224 2_3:-60275368 Msa0303130:intron
AATTGCTGCAACACAGAATG+TGG 0.594294 2_3:+60274090 None:intergenic
GAACTATTTCCTCCTTGGCA+AGG 0.595730 2_3:-60274132 Msa0303130:CDS
TTCAGAGTTTACAGGATGGG+AGG 0.596329 2_3:+60276251 None:intergenic
ATCTTACAAATGACAAAAGA+GGG 0.598252 2_3:-60269944 Msa0303130:three_prime_UTR
ATGGAGGGTAGATACAGGGG+AGG 0.603410 2_3:-60270104 Msa0303130:three_prime_UTR
GGTAAACAGAATGCTTAAGC+CGG 0.609194 2_3:-60279027 Msa0303130:CDS
GTGAAGATAGAGTATCAGAA+TGG 0.610806 2_3:-60276080 Msa0303130:CDS
ATGCATAGTAGAAGAAATGG+AGG 0.611017 2_3:-60270120 Msa0303130:three_prime_UTR
TATCCTTGCTGTCTATGCAT+GGG 0.611357 2_3:-60275872 Msa0303130:CDS
AATCATCAGACCTTTCATGA+TGG 0.618732 2_3:+60276601 None:intergenic
GATGGGAGGTAGTAGGAGGT+TGG 0.619222 2_3:+60276265 None:intergenic
TTTGCGTTCATCCTTAGAGG+AGG 0.621497 2_3:+60276725 None:intergenic
ACCGCCAATTTGTCGGACAT+GGG 0.622584 2_3:+60270261 None:intergenic
ACAGGAGAGTGAGAAAGGCA+AGG 0.623111 2_3:+60280030 None:intergenic
CCTGCTACGGGGTATTGCCA+AGG 0.624115 2_3:-60275844 Msa0303130:intron
CAACCCATGCATAGACAGCA+AGG 0.624154 2_3:+60275869 None:intergenic
ATAATAATAATATCAAGCAA+TGG 0.624389 2_3:-60280119 Msa0303130:CDS
CAATTTCAGAGTTTACAGGA+TGG 0.628288 2_3:+60276247 None:intergenic
CTTGTGTAATGCAGATGATG+TGG 0.631102 2_3:-60274462 Msa0303130:intron
GTTGAAGTAGAACAATGTCA+TGG 0.631167 2_3:-60276660 Msa0303130:intron
CAAGCCTTGTCTGATAGTGA+AGG 0.631974 2_3:-60278996 Msa0303130:CDS
CAAACACAGACCATCATGAA+AGG 0.632819 2_3:-60276611 Msa0303130:CDS
ACCAATCTGTTTGAAAAGAG+CGG 0.633077 2_3:-60276417 Msa0303130:intron
AGCCAATAAGAAGATTGACA+AGG 0.637025 2_3:-60270308 Msa0303130:exon
TGGAGGGTAGATACAGGGGA+GGG 0.640202 2_3:-60270103 Msa0303130:three_prime_UTR
ATCATCATCAGAATCAGCAA+CGG 0.647435 2_3:+60274336 None:intergenic
AGATGTACCACAGCCAGCAA+TGG 0.650736 2_3:-60276136 Msa0303130:intron
AAGATAGGGAGAGAACCAGA+TGG 0.650738 2_3:-60276489 Msa0303130:CDS
TTAATGAGGCTCTTTCAATG+TGG 0.665135 2_3:-60275372 Msa0303130:CDS
TTGTGTAATGCAGATGATGT+GGG 0.665739 2_3:-60274461 Msa0303130:intron
TGATCAGTGTTCACATGATG+CGG 0.666308 2_3:+60274250 None:intergenic
CCAATAAGAAGATTGACAAG+GGG 0.669030 2_3:-60270306 Msa0303130:exon
GTTTCCTTTCCTTGCCAAGG+AGG 0.672354 2_3:+60274123 None:intergenic
AAGCAATGCATTCAAGTGTG+GGG 0.673117 2_3:-60276797 Msa0303130:CDS
AGATTGGTGACAGGTAAAGG+AGG 0.675488 2_3:+60276432 None:intergenic
TTTCTCGAACGATCTTCTGG+CGG 0.685058 2_3:+60274056 None:intergenic
GCACTGCTGAGTATCGAACA+AGG 0.700502 2_3:-60277645 Msa0303130:CDS
TGCATAGTAGAAGAAATGGA+GGG 0.701410 2_3:-60270119 Msa0303130:three_prime_UTR
AGCCCATCCTTGAGACATCG+AGG 0.704356 2_3:+60276107 None:intergenic
CATGGGTTGATCCTGCTACG+GGG 0.708410 2_3:-60275855 Msa0303130:CDS
GTTTGTGATGTATTGTAACG+GGG 0.714306 2_3:-60280148 Msa0303130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGAAAGTGGTGAATGTGAAT+TGG + chr2_3:60270010-60270029 None:intergenic 35.0%
AATGGCTATTGGAAGAAGCA+TGG - chr2_3:60269918-60269937 Msa0303130:three_prime_UTR 40.0%
! CAAGCCTTATCCCACTAAGT+GGG - chr2_3:60277798-60277817 Msa0303130:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG - chr2_3:60277799-60277818 Msa0303130:intron 45.0%
CCACTTAGTGGGATAAGGCT+TGG + chr2_3:60277800-60277819 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG - chr2_3:60277797-60277816 Msa0303130:intron 50.0%
CGACCCCACTTAGTGGGATA+AGG + chr2_3:60270944-60270963 None:intergenic 55.0%
TGTAGCCGACCCCACTTAGT+GGG + chr2_3:60270938-60270957 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG + chr2_3:60270937-60270956 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 60269912 60280537 60269912 ID=Msa0303130;Name=Msa0303130
chr2_3 mRNA 60269912 60280537 60269912 ID=Msa0303130-mRNA-1;Parent=Msa0303130;Name=Msa0303130-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0.5|0.33|1|0.5|0.4|15|410|678
chr2_3 exon 60269912 60270328 60269912 ID=Msa0303130-mRNA-1:exon:19634;Parent=Msa0303130-mRNA-1
chr2_3 exon 60274049 60274224 60274049 ID=Msa0303130-mRNA-1:exon:19633;Parent=Msa0303130-mRNA-1
chr2_3 exon 60274246 60274470 60274246 ID=Msa0303130-mRNA-1:exon:19632;Parent=Msa0303130-mRNA-1
chr2_3 exon 60275369 60275569 60275369 ID=Msa0303130-mRNA-1:exon:19631;Parent=Msa0303130-mRNA-1
chr2_3 exon 60275649 60275737 60275649 ID=Msa0303130-mRNA-1:exon:19630;Parent=Msa0303130-mRNA-1
chr2_3 exon 60275845 60275967 60275845 ID=Msa0303130-mRNA-1:exon:19629;Parent=Msa0303130-mRNA-1
chr2_3 exon 60276058 60276145 60276058 ID=Msa0303130-mRNA-1:exon:19628;Parent=Msa0303130-mRNA-1
chr2_3 exon 60276179 60276411 60276179 ID=Msa0303130-mRNA-1:exon:19627;Parent=Msa0303130-mRNA-1
chr2_3 exon 60276418 60276639 60276418 ID=Msa0303130-mRNA-1:exon:19626;Parent=Msa0303130-mRNA-1
chr2_3 exon 60276661 60276851 60276661 ID=Msa0303130-mRNA-1:exon:19625;Parent=Msa0303130-mRNA-1
chr2_3 exon 60277628 60277731 60277628 ID=Msa0303130-mRNA-1:exon:19624;Parent=Msa0303130-mRNA-1
chr2_3 exon 60278992 60279048 60278992 ID=Msa0303130-mRNA-1:exon:19623;Parent=Msa0303130-mRNA-1
chr2_3 exon 60280026 60280187 60280026 ID=Msa0303130-mRNA-1:exon:19622;Parent=Msa0303130-mRNA-1
chr2_3 exon 60280200 60280212 60280200 ID=Msa0303130-mRNA-1:exon:19621;Parent=Msa0303130-mRNA-1
chr2_3 exon 60280392 60280537 60280392 ID=Msa0303130-mRNA-1:exon:19620;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60280392 60280537 60280392 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60280200 60280212 60280200 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60280026 60280187 60280026 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60278992 60279048 60278992 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60277628 60277731 60277628 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60276661 60276851 60276661 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60276418 60276639 60276418 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60276179 60276411 60276179 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60276058 60276145 60276058 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60275845 60275967 60275845 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60275649 60275737 60275649 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60275369 60275569 60275369 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60274246 60274470 60274246 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60274049 60274224 60274049 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 CDS 60270322 60270328 60270322 ID=Msa0303130-mRNA-1:cds;Parent=Msa0303130-mRNA-1
chr2_3 three_prime_UTR 60269912 60270321 60269912 ID=Msa0303130-mRNA-1:three_prime_utr;Parent=Msa0303130-mRNA-1
Gene Sequence

>Msa0303130

GGAAAAAATGGCTATTGGAAGAAGCATGGAGAAGAAGAAGCTCGCTCTTCTCTTTCTGAATCTATAAACTTCAATTTTCTCAGATCTCAATCATTCCAATTCACATTCACCACTTTCAATCATTCTTCTTCGATCCTTGCGTTTCGGAATGAAATGAAAGGTTTTGACATTGTTGAGTTTGTGATGTATTGTAACGGGGAAGATAATAATAATAATATCAAGCAATGGAGTTGTGGAAAAGCTGGTACTGTGAATTTGCGAAAAGTGAGCTCGTTCGTTCGCGATATTCGAGAGCCTTGCCTTTCTCACTCTCCTGTAAAGGTAAACAGAATGCTTAAGCCGGACATATGGCAAGCCTTGTCTGATAGTGAAGGAAAGGGTATAGATCCATCGATTAGACCCGAAGTTTGGGAGTTCTTACTAGGATGTTATGCACTGAGCAGCACTGCTGAGTATCGAACAAGGTTGAGAGCAGCTAGAAGGGAGCTTTATAGGGACCTGGTTAAGCAATGTCAAGCAATGCATTCAAGTGTGGGGACTGGTTCATTGGCATATATTGTTGGCTCCAAAGTTATGGATATGAGGACCTCCTCTAAGGATGAACGCAAAATTGAAGCTAAACTAGAAAGGTCAACATCTAATGATAATAATGTTGAAGTAGAACAATGTCATGAGGCAGCAAACACAGACCATCATGAAAGGTCTGATGATTGGGCTGACCAACTTAACCTAGTTAGTTCGAGAGCAAGTACAGATAGTGCAGCTTCTGATTTTTCTGGTCATCAAAACTGCAGTTCCCCAAAGATAGGGAGAGAACCAGATGGATCACATAATAGGATAGACAGTAATTTTGATTTTCCTCCTTTACCTGTCACCAATCTGTTTGAAAAGAGCGATAAAAACTCGGGCATAGAGCATGGCAATAAATTTTCTACACAGCGTAAACTAAGCTTTGAAGATGACAGAATGCATAGCTTCCATATCAACAACAACATAGACTTAGTTATTGAATCAAATGGCCAACCTCCTACTACCTCCCATCCTGTAAACTCTGAAATTGAAACTGCTTCACCTGATGAAGATGAACCAGACTTTCCATCTGACAATCCAGTTTATGAAACGCCTATGCCAGCAATGGCAAGTGCCTCGATGTCTCAAGGATGGGCTGCCAGTGAAGATAGAGTATCAGAATGGCTTTGGACTTTGCACAGGATAGTTGTTGATGTTGTGAGGACCGATAATCATCTTGAATTCTATGAGGACAGGAGAAATTTAGCTAGAATGTCAGATATCCTTGCTGTCTATGCATGGGTTGATCCTGCTACGGGGTATTGCCAAGGTATGAGTGATTTGCTGTCTCCCTTTGTTGTAATCTTTGAGGATAACGCAGATGCATTTTGGTGTTTTGAGATGCTTTTGAGGAGAATGAGAGAAAATTTTAAAATGGAAGGCCCAACTAGGGTGATGAAGCAGCTGCGAGCATTATGGCATATTCTCGAGCTGTCAGACAAAGAACTTTTTGCCCACCTGTCAAAAATTGGAGCTGAAAGCCTTCATTTTGCATTTCGTATGTTGCTAGTTCTCTTTCGGAGAGAGTTATCTTTTAATGAGGCTCTTTCAATGTGGGAGATGATGTGGGCAGCTGACTTTGATGAATCCTGGGCATTTGAGTTAGAGGAGAATTGTCTGGAAGCATTGGAATTACATCTTCCTAGAGACTCAAGCAATTATATTAAAGAAGCCGTTGCTGATTCTGATGATGATAGTATCAAGAGTGATTCACAGTCAAATCATAATGAAAATGACAACACAAAAGCCAGCCCGCAGCCGCATCATGTGAACACTGATCACCCTTTGCAAACATCCAATACCTTTTGTGGCTTGGCAAGGAATATCTGGTCAAGAAATGGTCGAGTTCAAACGAGAACTATTTCCTCCTTGGCAAGGAAAGGAAACGATGAGTTAGCCACATTCTGTGTTGCAGCAATTCTTGTTTTAAACCGCCAGAAGATCGTTCGAGAAACCAACTCCCAATAA
Protein sequence

>Msa0303130

GKNGYWKKHGEEEARSSLSESINFNFLRSQSFQFTFTTFNHSSSILAFRNEMKGFDIVEFVMYCNGEDNNNNIKQWSCGKAGTVNLRKVSSFVRDIREPCLSHSPVKVNRMLKPDIWQALSDSEGKGIDPSIRPEVWEFLLGCYALSSTAEYRTRLRAARRELYRDLVKQCQAMHSSVGTGSLAYIVGSKVMDMRTSSKDERKIEAKLERSTSNDNNVEVEQCHEAANTDHHERSDDWADQLNLVSSRASTDSAASDFSGHQNCSSPKIGREPDGSHNRIDSNFDFPPLPVTNLFEKSDKNSGIEHGNKFSTQRKLSFEDDRMHSFHINNNIDLVIESNGQPPTTSHPVNSEIETASPDEDEPDFPSDNPVYETPMPAMASASMSQGWAASEDRVSEWLWTLHRIVVDVVRTDNHLEFYEDRRNLARMSDILAVYAWVDPATGYCQGMSDLLSPFVVIFEDNADAFWCFEMLLRRMRENFKMEGPTRVMKQLRALWHILELSDKELFAHLSKIGAESLHFAFRMLLVLFRRELSFNEALSMWEMMWAADFDESWAFELEENCLEALELHLPRDSSNYIKEAVADSDDDSIKSDSQSNHNENDNTKASPQPHHVNTDHPLQTSNTFCGLARNIWSRNGRVQTRTISSLARKGNDELATFCVAAILVLNRQKIVRETNSQ*