AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0305020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0305020 MtrunA17_Chr2g0323741 73.171 82 22 0 1 82 1 82 6.82e-40 127
Msa0305020 MtrunA17_Chr2g0323751 89.062 64 7 0 1 64 1 64 4.42e-38 123
Msa0305020 MtrunA17_Chr2g0323761 81.667 60 10 1 1 60 1 59 1.10e-30 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGAGACGAGTTCGGTTT+TGG 0.261084 2_3:-62436484 None:intergenic
GCTGATTTGCACTTATGAAT+TGG 0.319739 2_3:-62436446 None:intergenic
AAACATGTGAAGGTTCTTCT+TGG 0.327112 2_3:+62437305 Msa0305020:intron
TGAGTTACTTAAACCGTGTT+TGG 0.337550 2_3:+62436384 Msa0305020:CDS
CATAAGTGCAAATCAGCTCT+TGG 0.437934 2_3:+62436452 Msa0305020:CDS
TGGCTTTCAAGATGCGTTGG+AGG 0.448982 2_3:+62437325 Msa0305020:CDS
AAACTTTGGTGTGGAGGAGC+TGG 0.481261 2_3:+62437352 Msa0305020:CDS
TCTTGGCTTTCAAGATGCGT+TGG 0.495531 2_3:+62437322 Msa0305020:CDS
GTTGTCCAGCGTCGTTGTAT+CGG 0.511337 2_3:+62436538 Msa0305020:CDS
GAATGCTGACAAAGCACCAC+CGG 0.532950 2_3:-62436510 None:intergenic
CGGCGGAGAAGAGACGAGTT+CGG 0.533512 2_3:-62436490 None:intergenic
GATGGCAGCAACCGTGGCTG+TGG 0.536890 2_3:+62436406 Msa0305020:CDS
GCAACCGTGGCTGTGGCACA+AGG 0.556834 2_3:+62436413 Msa0305020:CDS
ACTCGTCTCTTCTCCGCCGG+TGG 0.562949 2_3:+62436494 Msa0305020:CDS
GGCTGTAAACTTTGGTGTGG+AGG 0.576400 2_3:+62437346 Msa0305020:CDS
TTACTTAAACCGTGTTTGGA+TGG 0.578366 2_3:+62436388 Msa0305020:CDS
CGGTTGCTGCCATCCAAACA+CGG 0.578962 2_3:-62436397 None:intergenic
GTGGCCTTGTGCCACAGCCA+CGG 0.579913 2_3:-62436417 None:intergenic
TGCTTCCGATACAACGACGC+TGG 0.592056 2_3:-62436543 None:intergenic
CGAACTCGTCTCTTCTCCGC+CGG 0.592246 2_3:+62436491 Msa0305020:CDS
GCGTTGGAGGCTGTAAACTT+TGG 0.615087 2_3:+62437338 Msa0305020:CDS
GATACAACGACGCTGGACAA+CGG 0.631291 2_3:-62436536 None:intergenic
TGTTTGGATGGCAGCAACCG+TGG 0.633356 2_3:+62436400 Msa0305020:CDS
GGAGGCTGTAAACTTTGGTG+TGG 0.644788 2_3:+62437343 Msa0305020:CDS
TGCTGACAAAGCACCACCGG+CGG 0.666936 2_3:-62436507 None:intergenic
ACTTATGAATTGGATCAGTG+TGG 0.757374 2_3:-62436436 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCAAGAAAACAACATATA+GGG - chr2_3:62436709-62436728 None:intergenic 20.0%
!!! TTTTCTTGAATGAGAAATAT+TGG + chr2_3:62436718-62436737 Msa0305020:intron 20.0%
!!! TTGATGAGTATTTTTTCATT+TGG + chr2_3:62436995-62437014 Msa0305020:intron 20.0%
!! TGCTAAAAGTTAGTTAATTT+AGG + chr2_3:62437021-62437040 Msa0305020:intron 20.0%
!! AAATCAAACATATGCAAAAA+AGG + chr2_3:62437379-62437398 Msa0305020:CDS 20.0%
!! ATTCAAGAAAACAACATATA+GGG - chr2_3:62436709-62436728 None:intergenic 20.0%
!!! TTTTCTTGAATGAGAAATAT+TGG + chr2_3:62436718-62436737 Msa0305020:intron 20.0%
!!! TTGATGAGTATTTTTTCATT+TGG + chr2_3:62436995-62437014 Msa0305020:intron 20.0%
!! TGCTAAAAGTTAGTTAATTT+AGG + chr2_3:62437021-62437040 Msa0305020:intron 20.0%
!! AAATCAAACATATGCAAAAA+AGG + chr2_3:62437379-62437398 Msa0305020:CDS 20.0%
! AAGTTATGTACATGAATTGT+TGG + chr2_3:62436624-62436643 Msa0305020:intron 25.0%
! AGTTATGTACATGAATTGTT+GGG + chr2_3:62436625-62436644 Msa0305020:intron 25.0%
! CATTCAAGAAAACAACATAT+AGG - chr2_3:62436710-62436729 None:intergenic 25.0%
! TGAATGAGAAATATTGGAAA+AGG + chr2_3:62436724-62436743 Msa0305020:intron 25.0%
! AATATTGGAAAAGGAAACAA+TGG + chr2_3:62436733-62436752 Msa0305020:intron 25.0%
! ATATTGGAAAAGGAAACAAT+GGG + chr2_3:62436734-62436753 Msa0305020:intron 25.0%
! TGCAAAATTTCCTTGAATAT+TGG + chr2_3:62436839-62436858 Msa0305020:intron 25.0%
! TATACAAGTGAATGAAATCA+AGG + chr2_3:62436872-62436891 Msa0305020:intron 25.0%
! TCTAAGGAATATTTCATGAT+AGG - chr2_3:62436929-62436948 None:intergenic 25.0%
! GTAGACTTATTTATAGTCTA+AGG - chr2_3:62436945-62436964 None:intergenic 25.0%
!!! AATTTAGGTTTGTTAAGAGT+TGG + chr2_3:62437036-62437055 Msa0305020:intron 25.0%
! GTCAAGAAAATTGTTCAAAA+CGG - chr2_3:62437241-62437260 None:intergenic 25.0%
! AAGTTATGTACATGAATTGT+TGG + chr2_3:62436624-62436643 Msa0305020:intron 25.0%
! AGTTATGTACATGAATTGTT+GGG + chr2_3:62436625-62436644 Msa0305020:intron 25.0%
! CATTCAAGAAAACAACATAT+AGG - chr2_3:62436710-62436729 None:intergenic 25.0%
! TGAATGAGAAATATTGGAAA+AGG + chr2_3:62436724-62436743 Msa0305020:intron 25.0%
! AATATTGGAAAAGGAAACAA+TGG + chr2_3:62436733-62436752 Msa0305020:intron 25.0%
! ATATTGGAAAAGGAAACAAT+GGG + chr2_3:62436734-62436753 Msa0305020:intron 25.0%
! TGCAAAATTTCCTTGAATAT+TGG + chr2_3:62436839-62436858 Msa0305020:intron 25.0%
! TATACAAGTGAATGAAATCA+AGG + chr2_3:62436872-62436891 Msa0305020:intron 25.0%
! TCTAAGGAATATTTCATGAT+AGG - chr2_3:62436929-62436948 None:intergenic 25.0%
! GTAGACTTATTTATAGTCTA+AGG - chr2_3:62436945-62436964 None:intergenic 25.0%
!!! AATTTAGGTTTGTTAAGAGT+TGG + chr2_3:62437036-62437055 Msa0305020:intron 25.0%
! GTCAAGAAAATTGTTCAAAA+CGG - chr2_3:62437241-62437260 None:intergenic 25.0%
! AACTTTCTGAAGAGAATCAT+CGG - chr2_3:62436609-62436628 None:intergenic 30.0%
GTTATGTACATGAATTGTTG+GGG + chr2_3:62436626-62436645 Msa0305020:intron 30.0%
TAAACGTATCAAATTGTCCA+AGG - chr2_3:62436764-62436783 None:intergenic 30.0%
ATCTTCTTCGTTCAAATTGT+GGG + chr2_3:62436794-62436813 Msa0305020:intron 30.0%
TTTGACTCAACCAATATTCA+AGG - chr2_3:62436852-62436871 None:intergenic 30.0%
! ATTAGTGACTGTTTGTTAGT+AGG + chr2_3:62437070-62437089 Msa0305020:intron 30.0%
! AACTTTCTGAAGAGAATCAT+CGG - chr2_3:62436609-62436628 None:intergenic 30.0%
GTTATGTACATGAATTGTTG+GGG + chr2_3:62436626-62436645 Msa0305020:intron 30.0%
TAAACGTATCAAATTGTCCA+AGG - chr2_3:62436764-62436783 None:intergenic 30.0%
ATCTTCTTCGTTCAAATTGT+GGG + chr2_3:62436794-62436813 Msa0305020:intron 30.0%
TTTGACTCAACCAATATTCA+AGG - chr2_3:62436852-62436871 None:intergenic 30.0%
! ATTAGTGACTGTTTGTTAGT+AGG + chr2_3:62437070-62437089 Msa0305020:intron 30.0%
TGAGTTACTTAAACCGTGTT+TGG + chr2_3:62436384-62436403 Msa0305020:CDS 35.0%
TTACTTAAACCGTGTTTGGA+TGG + chr2_3:62436388-62436407 Msa0305020:CDS 35.0%
ACTTATGAATTGGATCAGTG+TGG - chr2_3:62436439-62436458 None:intergenic 35.0%
!! GCTGATTTGCACTTATGAAT+TGG - chr2_3:62436449-62436468 None:intergenic 35.0%
GCAAGCAGAAATGATTCTAA+GGG + chr2_3:62436672-62436691 Msa0305020:intron 35.0%
GATCTTCTTCGTTCAAATTG+TGG + chr2_3:62436793-62436812 Msa0305020:intron 35.0%
GCAACTTGAACAAATATTCC+TGG - chr2_3:62436822-62436841 None:intergenic 35.0%
AAAGCAAAGAGAAGCAAAAG+AGG + chr2_3:62436895-62436914 Msa0305020:intron 35.0%
AAGCAAAGAGAAGCAAAAGA+GGG + chr2_3:62436896-62436915 Msa0305020:intron 35.0%
TTGATCAGCTCACTAATCAT+TGG - chr2_3:62436973-62436992 None:intergenic 35.0%
CGAATACAACACATATGTAG+AGG - chr2_3:62437105-62437124 None:intergenic 35.0%
CTCTCTCTCATATGTTCTAA+TGG + chr2_3:62437169-62437188 Msa0305020:intron 35.0%
GTTCTAATGGTATGTTAGCT+CGG + chr2_3:62437182-62437201 Msa0305020:intron 35.0%
CATTCTTCTCTCATGTTCAA+TGG + chr2_3:62437260-62437279 Msa0305020:intron 35.0%
TAGATGACACAAACATGTGA+AGG + chr2_3:62437295-62437314 Msa0305020:intron 35.0%
AAACATGTGAAGGTTCTTCT+TGG + chr2_3:62437305-62437324 Msa0305020:intron 35.0%
TGAGTTACTTAAACCGTGTT+TGG + chr2_3:62436384-62436403 Msa0305020:CDS 35.0%
TTACTTAAACCGTGTTTGGA+TGG + chr2_3:62436388-62436407 Msa0305020:CDS 35.0%
ACTTATGAATTGGATCAGTG+TGG - chr2_3:62436439-62436458 None:intergenic 35.0%
!! GCTGATTTGCACTTATGAAT+TGG - chr2_3:62436449-62436468 None:intergenic 35.0%
GCAAGCAGAAATGATTCTAA+GGG + chr2_3:62436672-62436691 Msa0305020:intron 35.0%
GATCTTCTTCGTTCAAATTG+TGG + chr2_3:62436793-62436812 Msa0305020:intron 35.0%
GCAACTTGAACAAATATTCC+TGG - chr2_3:62436822-62436841 None:intergenic 35.0%
AAAGCAAAGAGAAGCAAAAG+AGG + chr2_3:62436895-62436914 Msa0305020:intron 35.0%
AAGCAAAGAGAAGCAAAAGA+GGG + chr2_3:62436896-62436915 Msa0305020:intron 35.0%
TTGATCAGCTCACTAATCAT+TGG - chr2_3:62436973-62436992 None:intergenic 35.0%
CGAATACAACACATATGTAG+AGG - chr2_3:62437105-62437124 None:intergenic 35.0%
CTCTCTCTCATATGTTCTAA+TGG + chr2_3:62437169-62437188 Msa0305020:intron 35.0%
GTTCTAATGGTATGTTAGCT+CGG + chr2_3:62437182-62437201 Msa0305020:intron 35.0%
CATTCTTCTCTCATGTTCAA+TGG + chr2_3:62437260-62437279 Msa0305020:intron 35.0%
TAGATGACACAAACATGTGA+AGG + chr2_3:62437295-62437314 Msa0305020:intron 35.0%
AAACATGTGAAGGTTCTTCT+TGG + chr2_3:62437305-62437324 Msa0305020:intron 35.0%
CATAAGTGCAAATCAGCTCT+TGG + chr2_3:62436452-62436471 Msa0305020:CDS 40.0%
TTTCTCCTCAGAACTCATGA+CGG - chr2_3:62436570-62436589 None:intergenic 40.0%
TACATGAATTGTTGGGGTCA+AGG + chr2_3:62436632-62436651 Msa0305020:intron 40.0%
GGCAAGCAGAAATGATTCTA+AGG + chr2_3:62436671-62436690 Msa0305020:intron 40.0%
AGGAAACAATGGGATTACCT+TGG + chr2_3:62436744-62436763 Msa0305020:intron 40.0%
AGCTCACTAATCATTGGCAA+TGG - chr2_3:62436967-62436986 None:intergenic 40.0%
TGCAAAAAAGGTAGCGTACA+AGG + chr2_3:62437391-62437410 Msa0305020:CDS 40.0%
AAAAAAGGTAGCGTACAAGG+AGG + chr2_3:62437394-62437413 Msa0305020:CDS 40.0%
CATAAGTGCAAATCAGCTCT+TGG + chr2_3:62436452-62436471 Msa0305020:CDS 40.0%
TTTCTCCTCAGAACTCATGA+CGG - chr2_3:62436570-62436589 None:intergenic 40.0%
TACATGAATTGTTGGGGTCA+AGG + chr2_3:62436632-62436651 Msa0305020:intron 40.0%
GGCAAGCAGAAATGATTCTA+AGG + chr2_3:62436671-62436690 Msa0305020:intron 40.0%
AGGAAACAATGGGATTACCT+TGG + chr2_3:62436744-62436763 Msa0305020:intron 40.0%
AGCTCACTAATCATTGGCAA+TGG - chr2_3:62436967-62436986 None:intergenic 40.0%
TGCAAAAAAGGTAGCGTACA+AGG + chr2_3:62437391-62437410 Msa0305020:CDS 40.0%
AAAAAAGGTAGCGTACAAGG+AGG + chr2_3:62437394-62437413 Msa0305020:CDS 40.0%
! AGAAGAGACGAGTTCGGTTT+TGG - chr2_3:62436487-62436506 None:intergenic 45.0%
ATTGTTGGGGTCAAGGCTAA+TGG + chr2_3:62436639-62436658 Msa0305020:intron 45.0%
CAAGGCTAATGGTGACAACA+AGG + chr2_3:62436650-62436669 Msa0305020:intron 45.0%
TCGTTCAAATTGTGGGATCC+AGG + chr2_3:62436801-62436820 Msa0305020:intron 45.0%
! TCTTGGCTTTCAAGATGCGT+TGG + chr2_3:62437322-62437341 Msa0305020:CDS 45.0%
! AGAAGAGACGAGTTCGGTTT+TGG - chr2_3:62436487-62436506 None:intergenic 45.0%
ATTGTTGGGGTCAAGGCTAA+TGG + chr2_3:62436639-62436658 Msa0305020:intron 45.0%
CAAGGCTAATGGTGACAACA+AGG + chr2_3:62436650-62436669 Msa0305020:intron 45.0%
TCGTTCAAATTGTGGGATCC+AGG + chr2_3:62436801-62436820 Msa0305020:intron 45.0%
! TCTTGGCTTTCAAGATGCGT+TGG + chr2_3:62437322-62437341 Msa0305020:CDS 45.0%
! GAATGCTGACAAAGCACCAC+CGG - chr2_3:62436513-62436532 None:intergenic 50.0%
GATACAACGACGCTGGACAA+CGG - chr2_3:62436539-62436558 None:intergenic 50.0%
GTTGTCCAGCGTCGTTGTAT+CGG + chr2_3:62436538-62436557 Msa0305020:CDS 50.0%
AGCAACCGTCATGAGTTCTG+AGG + chr2_3:62436562-62436581 Msa0305020:intron 50.0%
TGGCTTTCAAGATGCGTTGG+AGG + chr2_3:62437325-62437344 Msa0305020:CDS 50.0%
GCGTTGGAGGCTGTAAACTT+TGG + chr2_3:62437338-62437357 Msa0305020:CDS 50.0%
GGAGGCTGTAAACTTTGGTG+TGG + chr2_3:62437343-62437362 Msa0305020:CDS 50.0%
! GGCTGTAAACTTTGGTGTGG+AGG + chr2_3:62437346-62437365 Msa0305020:CDS 50.0%
! AAACTTTGGTGTGGAGGAGC+TGG + chr2_3:62437352-62437371 Msa0305020:CDS 50.0%
! GAATGCTGACAAAGCACCAC+CGG - chr2_3:62436513-62436532 None:intergenic 50.0%
GATACAACGACGCTGGACAA+CGG - chr2_3:62436539-62436558 None:intergenic 50.0%
GTTGTCCAGCGTCGTTGTAT+CGG + chr2_3:62436538-62436557 Msa0305020:CDS 50.0%
AGCAACCGTCATGAGTTCTG+AGG + chr2_3:62436562-62436581 Msa0305020:intron 50.0%
TGGCTTTCAAGATGCGTTGG+AGG + chr2_3:62437325-62437344 Msa0305020:CDS 50.0%
GCGTTGGAGGCTGTAAACTT+TGG + chr2_3:62437338-62437357 Msa0305020:CDS 50.0%
GGAGGCTGTAAACTTTGGTG+TGG + chr2_3:62437343-62437362 Msa0305020:CDS 50.0%
! GGCTGTAAACTTTGGTGTGG+AGG + chr2_3:62437346-62437365 Msa0305020:CDS 50.0%
! AAACTTTGGTGTGGAGGAGC+TGG + chr2_3:62437352-62437371 Msa0305020:CDS 50.0%
CGGTTGCTGCCATCCAAACA+CGG - chr2_3:62436400-62436419 None:intergenic 55.0%
TGTTTGGATGGCAGCAACCG+TGG + chr2_3:62436400-62436419 Msa0305020:CDS 55.0%
TGCTTCCGATACAACGACGC+TGG - chr2_3:62436546-62436565 None:intergenic 55.0%
CGGTTGCTGCCATCCAAACA+CGG - chr2_3:62436400-62436419 None:intergenic 55.0%
TGTTTGGATGGCAGCAACCG+TGG + chr2_3:62436400-62436419 Msa0305020:CDS 55.0%
TGCTTCCGATACAACGACGC+TGG - chr2_3:62436546-62436565 None:intergenic 55.0%
CGGCGGAGAAGAGACGAGTT+CGG - chr2_3:62436493-62436512 None:intergenic 60.0%
CGAACTCGTCTCTTCTCCGC+CGG + chr2_3:62436491-62436510 Msa0305020:CDS 60.0%
! TGCTGACAAAGCACCACCGG+CGG - chr2_3:62436510-62436529 None:intergenic 60.0%
CGGCGGAGAAGAGACGAGTT+CGG - chr2_3:62436493-62436512 None:intergenic 60.0%
CGAACTCGTCTCTTCTCCGC+CGG + chr2_3:62436491-62436510 Msa0305020:CDS 60.0%
! TGCTGACAAAGCACCACCGG+CGG - chr2_3:62436510-62436529 None:intergenic 60.0%
GATGGCAGCAACCGTGGCTG+TGG + chr2_3:62436406-62436425 Msa0305020:CDS 65.0%
GCAACCGTGGCTGTGGCACA+AGG + chr2_3:62436413-62436432 Msa0305020:CDS 65.0%
!! GTGGCCTTGTGCCACAGCCA+CGG - chr2_3:62436420-62436439 None:intergenic 65.0%
ACTCGTCTCTTCTCCGCCGG+TGG + chr2_3:62436494-62436513 Msa0305020:CDS 65.0%
! GCTCGGTTCGATCGCGACGA+AGG + chr2_3:62437199-62437218 Msa0305020:intron 65.0%
GATGGCAGCAACCGTGGCTG+TGG + chr2_3:62436406-62436425 Msa0305020:CDS 65.0%
GCAACCGTGGCTGTGGCACA+AGG + chr2_3:62436413-62436432 Msa0305020:CDS 65.0%
!! GTGGCCTTGTGCCACAGCCA+CGG - chr2_3:62436420-62436439 None:intergenic 65.0%
ACTCGTCTCTTCTCCGCCGG+TGG + chr2_3:62436494-62436513 Msa0305020:CDS 65.0%
! GCTCGGTTCGATCGCGACGA+AGG + chr2_3:62437199-62437218 Msa0305020:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 62436383 62437463 62436383 ID=Msa0305020;Name=Msa0305020
chr2_3 mRNA 62436383 62437463 62436383 ID=Msa0305020-mRNA-1;Parent=Msa0305020;Name=Msa0305020-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|0|0|2|0|110
chr2_3 exon 62436383 62436568 62436383 ID=Msa0305020-mRNA-1:exon:20861;Parent=Msa0305020-mRNA-1
chr2_3 exon 62437317 62437463 62437317 ID=Msa0305020-mRNA-1:exon:20862;Parent=Msa0305020-mRNA-1
chr2_3 CDS 62436383 62436568 62436383 ID=Msa0305020-mRNA-1:cds;Parent=Msa0305020-mRNA-1
chr2_3 CDS 62437317 62437463 62437317 ID=Msa0305020-mRNA-1:cds;Parent=Msa0305020-mRNA-1
Gene Sequence

>Msa0305020

ATGAGTTACTTAAACCGTGTTTGGATGGCAGCAACCGTGGCTGTGGCACAAGGCCACACTGATCCAATTCATAAGTGCAAATCAGCTCTTGGAACTATTCACCAAAACCGAACTCGTCTCTTCTCCGCCGGTGGTGCTTTGTCAGCATTCTTGCCGTTGTCCAGCGTCGTTGTATCGGAAGCAACCGTTCTTCTTGGCTTTCAAGATGCGTTGGAGGCTGTAAACTTTGGTGTGGAGGAGCTGGTTGAAAATCAAACATATGCAAAAAAGGTAGCGTACAAGGAGGTTGAAGAAAAAGTAATACAAGTGTTGTTTTCATTCATGAATGCGTGA

Protein sequence

>Msa0305020

MSYLNRVWMAATVAVAQGHTDPIHKCKSALGTIHQNRTRLFSAGGALSAFLPLSSVVVSEATVLLGFQDALEAVNFGVEELVENQTYAKKVAYKEVEEKVIQVLFSFMNA*