AlfalfaGEDB | Msa0306080

M. sativa cultivar zhongmu-4 / Msa0306080

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0306080	XP_039686723.1	64.147	1085	214	13	426	1342	242	1319	0.0	1115
Msa0306080	XP_039686723.1	64.778	1059	257	11	1	948	1	1054	0.0	1070

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0068680	Msa0306080	0.808461	3.062611e-50	-8.615850e-47
Msa0159930	Msa0306080	0.801715	7.898531e-49	-8.615850e-47
Msa0227250	Msa0306080	0.933033	3.472704e-95	-8.615850e-47
Msa0239800	Msa0306080	0.803058	4.179080e-49	-8.615850e-47
Msa0262600	Msa0306080	0.928529	2.538641e-92	-8.615850e-47
Msa0306080	Msa0338090	0.934071	7.120367e-96	-8.615850e-47
Msa0306080	Msa0338170	0.937481	3.229510e-98	-8.615850e-47
Msa0306080	Msa0631200	0.802260	6.104839e-49	-8.615850e-47
Msa0306080	Msa1395900	0.803601	3.225101e-49	-8.615850e-47
Msa0306080	Msa1421300	0.951388	2.269267e-109	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0306080	MtrunA17_Chr2g0325361	71.201	816	158	6	604	1342	262	1077	0.0	973
Msa0306080	MtrunA17_Chr2g0325361	66.385	827	227	8	1	798	1	805	0.0	862
Msa0306080	MtrunA17_Chr2g0325281	64.094	596	160	5	1	556	1	582	0.0	645
Msa0306080	MtrunA17_Chr2g0325281	50.000	374	158	5	756	1124	252	601	1.42e-75	263
Msa0306080	MtrunA17_Chr2g0325281	44.048	420	180	4	545	953	211	586	5.58e-71	250
Msa0306080	MtrunA17_Chr2g0325281	43.229	384	175	4	398	778	239	582	8.36e-61	220
Msa0306080	MtrunA17_Chr2g0325281	43.840	349	180	2	479	825	253	587	9.48e-60	217
Msa0306080	MtrunA17_Chr2g0325281	46.491	342	164	3	674	1013	252	576	2.11e-57	210
Msa0306080	MtrunA17_Chr2g0325281	44.318	352	163	4	563	898	253	587	3.07e-57	209
Msa0306080	MtrunA17_Chr2g0325281	39.056	233	113	3	244	447	379	611	2.80e-22	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 273 sgRNAs with CRISPR-Local

Find 387 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAGCATTTCAAAACT+TGG	0.405649	2_3:+64326809	Msa0306080:CDS
AAAATTGCCGTGGAGCTGTT+TGG	0.424002	2_3:+64330260	Msa0306080:CDS
AAACTCTGTTTGCAAAACCC+AGG	0.651184	2_3:-64330286	None:intergenic
AAAGCAGCAGTAAATTCCAT+AGG	0.527911	2_3:-64331622	None:intergenic
AAAGGAGCATTTCAAAACTT+GGG	0.539886	2_3:+64326810	Msa0306080:CDS
AAATAAGCTAATGTAAGAAA+TGG	0.371185	2_3:+64331475	Msa0306080:three_prime_UTR
AACAATTGCTGAACTTGCTT+TGG	0.297042	2_3:+64326471	Msa0306080:CDS
AACCAACTCCGTTGAAATCA+AGG	0.446102	2_3:+64331189	Msa0306080:CDS
AACCTTGATTTCAACGGAGT+TGG	0.457408	2_3:-64331191	None:intergenic
AAGAGAAGCAGTGTGAAGAA+CGG	0.581369	2_3:+64326683	Msa0306080:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATAGAACTTAATCAAGTCA+AGG	+	chr2_3:64326354-64326373	Msa0306080:CDS	30.0%
CAGAAAAGTTAGAATCTGAA+AGG	+	chr2_3:64326551-64326570	Msa0306080:CDS	30.0%
GAATAGTATTGCTCAAATGA+AGG	+	chr2_3:64326606-64326625	Msa0306080:CDS	30.0%
TTGATTCAAATTCTTTCGCA+CGG	-	chr2_3:64326747-64326766	None:intergenic	30.0%
TGCGAAAGAATTTGAATCAA+AGG	+	chr2_3:64326747-64326766	Msa0306080:CDS	30.0%
AGAATTTGAATCAAAGGAGA+AGG	+	chr2_3:64326753-64326772	Msa0306080:CDS	30.0%
TCAAAAGAGAGAGAATTTGA+AGG	+	chr2_3:64327342-64327361	Msa0306080:CDS	30.0%
TCAAAAGAGAGTCAATTTGA+AGG	+	chr2_3:64327426-64327445	Msa0306080:CDS	30.0%
AGAGTTTCAATCAAAAGAAG+AGG	+	chr2_3:64327458-64327477	Msa0306080:CDS	30.0%
TCAAAAGAGAGTCAATTTGA+AGG	+	chr2_3:64327426-64327445	Msa0306080:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	64325822	64331768	64325822	ID=Msa0306080;Name=Msa0306080
chr2_3	mRNA	64325822	64331768	64325822	ID=Msa0306080-mRNA-1;Parent=Msa0306080;Name=Msa0306080-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0\|0.42\|0.25\|1\|1\|1\|8\|305\|1343
chr2_3	exon	64325822	64326205	64325822	ID=Msa0306080-mRNA-1:exon:21484;Parent=Msa0306080-mRNA-1
chr2_3	exon	64326274	64327998	64326274	ID=Msa0306080-mRNA-1:exon:21485;Parent=Msa0306080-mRNA-1
chr2_3	exon	64328088	64328618	64328088	ID=Msa0306080-mRNA-1:exon:21486;Parent=Msa0306080-mRNA-1
chr2_3	exon	64328651	64329368	64328651	ID=Msa0306080-mRNA-1:exon:21487;Parent=Msa0306080-mRNA-1
chr2_3	exon	64329818	64330087	64329818	ID=Msa0306080-mRNA-1:exon:21488;Parent=Msa0306080-mRNA-1
chr2_3	exon	64330222	64330311	64330222	ID=Msa0306080-mRNA-1:exon:21489;Parent=Msa0306080-mRNA-1
chr2_3	exon	64331038	64331210	64331038	ID=Msa0306080-mRNA-1:exon:21490;Parent=Msa0306080-mRNA-1
chr2_3	exon	64331323	64331768	64331323	ID=Msa0306080-mRNA-1:exon:21491;Parent=Msa0306080-mRNA-1
chr2_3	CDS	64325822	64326205	64325822	ID=Msa0306080-mRNA-1:cds;Parent=Msa0306080-mRNA-1
chr2_3	CDS	64326274	64327998	64326274	ID=Msa0306080-mRNA-1:cds;Parent=Msa0306080-mRNA-1
chr2_3	CDS	64328088	64328618	64328088	ID=Msa0306080-mRNA-1:cds;Parent=Msa0306080-mRNA-1
chr2_3	CDS	64328651	64329368	64328651	ID=Msa0306080-mRNA-1:cds;Parent=Msa0306080-mRNA-1
chr2_3	CDS	64329818	64330087	64329818	ID=Msa0306080-mRNA-1:cds;Parent=Msa0306080-mRNA-1
chr2_3	CDS	64330222	64330311	64330222	ID=Msa0306080-mRNA-1:cds;Parent=Msa0306080-mRNA-1
chr2_3	CDS	64331038	64331210	64331038	ID=Msa0306080-mRNA-1:cds;Parent=Msa0306080-mRNA-1
chr2_3	CDS	64331323	64331463	64331323	ID=Msa0306080-mRNA-1:cds;Parent=Msa0306080-mRNA-1
chr2_3	three_prime_UTR	64331464	64331768	64331464	ID=Msa0306080-mRNA-1:three_prime_utr;Parent=Msa0306080-mRNA-1

Gene Sequence

>Msa0306080

ATGGATTTTGAGCCTTCTGATGATGGGAGGGAAACTGTTCCTTTGTTACCAACGTTTTCCCGGGTTTCGAGTTTCGAAAAATGTAGTAGTAGTAGTGCTGTTAGAAAGAGGGGTTCTGAGTTTGATGACAACAATTCATTGCATATTTCTGTTAAGAAATCAAAGTTGTTGCCCTACGACGATCACGACAAAGATGGACATGATATTTCATTGTCGAAGAAATTGACTGATAAATCATTTTCCTCGCTGAAGAAAGACCTTACGTTGTTAGAACAATTATTTGCGGAATGTAAAAGGAAGCAGAAAGTTGAAGAGAAGAGATTACAGTCCATAAAGAGAGATACTGAAGAGTGCTTCAAAGAGCTTCAAATTAAGAAGAAGCAAGAGTTTGTAGTGAAAGAAGAGGAGCTCTATTTGATGGAAAACTTGATTAAAGAGAGCAAGCTTGAGCTCAAGGCAAAAGAGATAGAACTTAATCAAGTCAAGGGTAACATTTCAAAAGAAATAGAACTTCGTCAAGTCATTGATAACATTGATAAGGACCGTGAAAGGAAGGAACAGGAACTCAAGGCTCTTTCCCAAACAATTGCTGAACTTGCTTTGGACTGTAAGGCCAAAGAGAACGAGCTTGATGCAGTTAACAAATTAATTGGTGAACAAGCAGAAAAGTTAGAATCTGAAAGGAAGAAGGCAATAAATATAATATCAGACATGAAGAATAGTATTGCTCAAATGAAGGAATTTGAGTCAAAGCAGAAGCGATTTGATGATCGGTTTAAGGAACTTGAGTCAAAAGAGAAGCAGTGTGAAGAACGGGTAACAAAGCTGGATTCAAGAGAGAAGCAGCTTGAAGGCCGTGCGAAAGAATTTGAATCAAAGGAGAAGGAACTTGAAGGTCGTATGAATGAGCTTGAATCAGAAAAGGAGCATTTCAAAACTTGGGTGAAGGAGCTTGATACAAAAGAGAAGCAAGTTGAAGGACGAGCAATGAAGCTAGTGTTAAAAGAGAAGCAGAGTGAAGGACGAGCGCAGGAGCTTCAGTCGAAGGAGAAGCACTTTGAAGAATGGGCAAAGAAGCTGGAGTCAAGAGAGAAGCAGCTTGATGTCCGTTTGATTGAATTTGAATTAAACGAGAAGGAACTTGAAGGTCGTATGAATGAGCGTGATAAAAAAGAGAAGCAAGTTGAAGGACGAGCAATAGAGCTAGAGTCAAAAGTGGTGCAACATGAAGGCCGGGTGAAGGAGTTTGAATCAAAGGAGAAGGAATTTGATGGCCGGGTGGAATCTAAAAAGAAGCATTTCGAAAGCATGATGGAGGAGCTCAAATCAAGAATGAGTTCGTTGAAAGGACAAGTTGAGGAACTTGACTCTAAAGAGAAGCAACTTGATGTCCATGTGAAGGAGCTGGAATCTAAAAGGAACCACTTCGAAAGACAGGTGAAGGAGTTTGAATCAAAAGAGAGAGAATTTGAAGGCCAAATGAAGGATTTGGAATCAGGAAGGAAGCATTTTGAAAGCCAAGTGGACGAGCTCAATTCAAAAGAGAGTCAATTTGAAGGACGGGTGAAAGAGTTTCAATCAAAAGAAGAGGAATTTAAAGGTCAAGTGAAGGAGTTTGAATCAAAGAAGGAGGAATTTGAAGGTCGATTGAAGGAGCATGGATCAAAGGAGGAGGAACTTGAACGCCGAGTGAAGGATTTTGAATCAGGAAGGAAGCATTTTGATAACCAAGTGGACGAGCTCAAGTCAAAAGAGAGTCAATTTGAAGGACAGGTGAAGGAGTTTCAATCAAAAGAGGAGGAATTTAAAGGTCAAGTGAAGGAGTTTGAATCAAAGAAGGAGGAATTTGAAGGTCGATTGAAGGAGCATGAATCAAAGGAGGAGGAACTTGAAGGCCGAGTGAAGGATTTTGAATCAGGAAGGAAGCATTTTGAGAGCCAAGTGGACCAGCTCAAGTCAAAAGAGAGGCAATTTGAAGGACTGGTGAAGGAGTTTCAATCACAAGAGGAGGAATTTAAAGGTCAAGTGAAGGAGTTTGAATCAAAGAAGGAGGAATTTGAAGGTCGATTGAAGGAGCATGAATCAAAGGAGGAGGAACTTGAAGGCCGAGAGTTTCAATCACAAGAGGAGGAATTTAAAGATCAAGTGAAGGAGTTTGAATCAAAGAAGGAGGAATTTGAAGGTCGATTGAAGGAGCATGAATCAAAGGAGGAGGAACTTGAAGGCCGAGTGAAGGATTTTGAATCAGGAAGGAAGCATTTTGAAAGCCAAGTGGACCAGCTCAAGTCAAAAGAGAGGCAATTTGAAGGACTCGTGAAGGAGTTTCAATCAAAAGAGGAGGAATTTAAAGGTCAAGTGAAGGAGTTTGAATCAAAGAAGGAGGAATTTGAAGGTCGATTGAAGGAGCGTGAATCAAAGGAGAAGGAATTTGAAGGCCGAGTGAAGGAGCTGGAATCTAAAAGGGAGCATTTCGAAAGCATGAGGAAGGAGCTCAAATCAAAATTGAGGCCATTGATAGAACAAGCTAAGGAACTTGACTCTAAAGAGAAGCAACTTGATAGCCAAGTGAAGGAGCTGGAATCTAAAAAGAAGCTGTTTGAAAGCCAAGTGAAGGAGTTACAGTCTAAAGATAACCAACTTAGGGAATTTGAAGGCCAAGCGAAGCAGCTGGAATCTAAAAGGAAGCATTTCGAAAGCCAAATGAAGAAATTTGAGTCTAAAGAGAAGCAACTTGAAGGCCGAGTGAAGGAGCATAAATCAAAAGAGAGAGGATTCGAAGGCCAACTGAAGGAACTGGAATCTAAAAAGAAGCATTTTGAAAATCAAGTGGAGGAGTTCAAATCAAAACAGAAGCAATTGAAAGGACAGGTTAAGGAACTTGAGTCTAAAGAGAAGCATTTTGATGGCCGAGTGAAGGAGCTTGAATCTAAAGAGGATGACTTTGAAGGCCGAGTGAAGAAGCATGAATCAAAAGAGAGAGAATTTGAAATTCTAGTGAAGGAGCTGCAATCTAAAAAGAAGCAAGTTGAAAACCAAGTTGAGTTAGAGTCAAAAGATAACCAACTTATATGCAAAATAAAGGAGTTTGAATCAAAAGAGAAGGAATTTGAAGAACAAGTGAAGGGCATAAAATCAATGGAGGAGGAACTCGAATCAAAAAAGAAGCATTTCGAAAGCCAAGTTGAGGACTTTAAATCAAAAGAGAAGCAATTTGAAGAACGATGGAATAAACTTGAGTCAGAAGAGAATAAGTTCAAAGCAAAGGTGAAGGAGCTCAACTTACAAGAAAAGTGGTTTGAAGGACAAGTCAAGGATCCTGTGTCAAGGAAAAAATATATTGATGGGGAAAAAGAATCAGTTGCATCTTACATGGATGATCATTCAAGTCGTGCCTTTGGTGGAATAAGTTTGCAATTGGGCGCAAGCAAGAAAACTGATGGAGTTGAGTCACTTTTCAACGGTGTTTTAGTTAATCTGCAAGAATCATCCGATCCATCAAAACTTGTTTTGGAAATGATACTGAACCCCATAATTCCAGAGGGTCAAAAGGGAGATAATGTGATTATTACTGATTACCAAATCTATCTGCTGGAACAACTGCTGAGAATCTCACCAGATATTGAACCTTATGAAGTTCTGGATCTTTTTGCACCTGTTGCTCTGCACAAAATTGCCGTGGAGCTGTTTGGATGCCTGGGTTTTGCAAACAGAGTTTCTGATTTTGTTAAGCGTCTTATCATGAGGAAGCAAATTGTTGAAGCTGTTCAATTCAGTTGTGCATATAACTTGACTGACAAGGATCAATTAGTTGATATGTTGCGAGAACACGTCCAGAATGCCAAACTGATTTGTGAGAGCAGTTGCAAGAAAACCAACTCCGTTGAAATCAAGGATCAGGCCAGAGATCAAGAAATTGCTAGTCTGGGGACTGTTCTACAATGCATTTCGGACAACAGCCTAGAATCTGCGGGTCTGCTTCATAAGGAGATCGACTATCGCATTCTTGAGCTAAAAGCACATAAAGGTTTTTAG

Protein sequence

>Msa0306080

MDFEPSDDGRETVPLLPTFSRVSSFEKCSSSSAVRKRGSEFDDNNSLHISVKKSKLLPYDDHDKDGHDISLSKKLTDKSFSSLKKDLTLLEQLFAECKRKQKVEEKRLQSIKRDTEECFKELQIKKKQEFVVKEEELYLMENLIKESKLELKAKEIELNQVKGNISKEIELRQVIDNIDKDRERKEQELKALSQTIAELALDCKAKENELDAVNKLIGEQAEKLESERKKAINIISDMKNSIAQMKEFESKQKRFDDRFKELESKEKQCEERVTKLDSREKQLEGRAKEFESKEKELEGRMNELESEKEHFKTWVKELDTKEKQVEGRAMKLVLKEKQSEGRAQELQSKEKHFEEWAKKLESREKQLDVRLIEFELNEKELEGRMNERDKKEKQVEGRAIELESKVVQHEGRVKEFESKEKEFDGRVESKKKHFESMMEELKSRMSSLKGQVEELDSKEKQLDVHVKELESKRNHFERQVKEFESKEREFEGQMKDLESGRKHFESQVDELNSKESQFEGRVKEFQSKEEEFKGQVKEFESKKEEFEGRLKEHGSKEEELERRVKDFESGRKHFDNQVDELKSKESQFEGQVKEFQSKEEEFKGQVKEFESKKEEFEGRLKEHESKEEELEGRVKDFESGRKHFESQVDQLKSKERQFEGLVKEFQSQEEEFKGQVKEFESKKEEFEGRLKEHESKEEELEGREFQSQEEEFKDQVKEFESKKEEFEGRLKEHESKEEELEGRVKDFESGRKHFESQVDQLKSKERQFEGLVKEFQSKEEEFKGQVKEFESKKEEFEGRLKERESKEKEFEGRVKELESKREHFESMRKELKSKLRPLIEQAKELDSKEKQLDSQVKELESKKKLFESQVKELQSKDNQLREFEGQAKQLESKRKHFESQMKKFESKEKQLEGRVKEHKSKERGFEGQLKELESKKKHFENQVEEFKSKQKQLKGQVKELESKEKHFDGRVKELESKEDDFEGRVKKHESKEREFEILVKELQSKKKQVENQVELESKDNQLICKIKEFESKEKEFEEQVKGIKSMEEELESKKKHFESQVEDFKSKEKQFEERWNKLESEENKFKAKVKELNLQEKWFEGQVKDPVSRKKYIDGEKESVASYMDDHSSRAFGGISLQLGASKKTDGVESLFNGVLVNLQESSDPSKLVLEMILNPIIPEGQKGDNVIITDYQIYLLEQLLRISPDIEPYEVLDLFAPVALHKIAVELFGCLGFANRVSDFVKRLIMRKQIVEAVQFSCAYNLTDKDQLVDMLREHVQNAKLICESSCKKTNSVEIKDQARDQEIASLGTVLQCISDNSLESAGLLHKEIDYRILELKAHKGF*