AlfalfaGEDB | Msa0308950

M. sativa cultivar zhongmu-4 / Msa0308950

KEGG: -
GO: GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0044212 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141
PFAM: PF03634.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0308950	XP_003592963.1	91.171	521	10	10	1	519	1	487	0.0	915

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0308950	sp\|Q9C518\|TCP8_ARATH	46.528	432	132	18	69	476	8	364	7.98e-80	258

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0308950	G7INN6	91.171	521	10	10	1	519	1	487	0.0	915

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TFs/TRs:

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Gene ID	Type	Classification
Msa0308950	TF	TCP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0188330	Msa0308950	0.962466	6.546359e-121	-8.615850e-47
Msa0270590	Msa0308950	0.842000	3.377571e-58	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0308950	MtrunA17_Chr2g0277361	93.548	465	7	9	1	463	1	444	0.0	843
Msa0308950	MtrunA17_Chr5g0416581	92.045	88	6	1	129	215	61	148	2.07e-49	174
Msa0308950	MtrunA17_Chr4g0060601	65.909	132	34	3	74	203	44	166	1.69e-45	165
Msa0308950	MtrunA17_Chr1g0211941	80.488	82	13	1	125	206	27	105	4.46e-38	140
Msa0308950	MtrunA17_Chr7g0224581	53.378	148	59	3	110	253	25	166	7.50e-38	140
Msa0308950	MtrunA17_Chr1g0204001	59.292	113	42	1	110	222	44	152	4.25e-36	137
Msa0308950	MtrunA17_Chr1g0164881	75.000	84	18	1	119	202	20	100	1.14e-35	133
Msa0308950	MtrunA17_Chr8g0350941	78.947	76	16	0	131	206	77	152	2.08e-35	135
Msa0308950	MtrunA17_Chr1g0180911	80.282	71	14	0	126	196	41	111	8.11e-35	129

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0308950	AT1G58100.1	46.528	432	132	18	69	476	8	364	8.11e-81	258
Msa0308950	AT1G35560.1	42.899	338	94	10	131	462	52	296	4.45e-59	199
Msa0308950	AT1G58100.2	41.204	432	131	19	69	476	8	340	3.55e-57	195
Msa0308950	AT1G72010.1	40.604	298	104	10	123	397	52	299	5.07e-48	171
Msa0308950	AT1G69690.1	58.599	157	38	3	129	273	46	187	1.50e-41	152
Msa0308950	AT5G23280.1	77.108	83	16	1	124	206	32	111	5.19e-37	137
Msa0308950	AT2G45680.1	62.500	112	40	1	102	211	41	152	1.87e-36	139
Msa0308950	AT5G08330.1	68.750	96	27	1	109	204	9	101	1.17e-35	133
Msa0308950	AT3G27010.2	52.756	127	43	2	69	195	30	139	2.81e-34	132
Msa0308950	AT3G27010.1	52.756	127	43	2	69	195	30	139	2.81e-34	132

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Find 149 sgRNAs with CRISPR-Local

Find 328 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACAATCAAAATGAAAG+TGG	0.537872	2_4:+3400002	Msa0308950:CDS
AACAATCAAAATGAAAGTGG+CGG	0.649002	2_4:+3400005	Msa0308950:CDS
AACAGCCATGTGGGCTACAG+CGG	0.560758	2_4:+3400193	Msa0308950:CDS
AACGTCTCACTCCGTAGCAG+CGG	0.639266	2_4:+3399732	Msa0308950:CDS
AACTTCAACCGCCGTCACCG+CGG	0.629301	2_4:+3399482	Msa0308950:CDS
AAGAGGAGGTTCAGATCTGG+TGG	0.633145	2_4:-3399409	None:intergenic
AAGAGTTGTTAACGTTGAAA+TGG	0.427409	2_4:-3400556	None:intergenic
AAGGCCATCACCTTTCATTA+TGG	0.235639	2_4:+3399266	Msa0308950:CDS
AATGTTCCAGCTTCTGGTGG+TGG	0.463360	2_4:+3400392	Msa0308950:CDS
ACACCTCATTGATGCTTCCC+TGG	0.479655	2_4:+3399380	Msa0308950:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTTGAAGAAGGTTGAATAT+TGG	-	chr2_4:3399301-3399320	None:intergenic	30.0%
ACGAGAATTACATGAGAAAA+CGG	+	chr2_4:3399952-3399971	Msa0308950:CDS	30.0%
AACAATCAAAATGAAAGTGG+CGG	+	chr2_4:3400005-3400024	Msa0308950:CDS	30.0%
AAGAGTTGTTAACGTTGAAA+TGG	-	chr2_4:3400559-3400578	None:intergenic	30.0%
TCAACAACATTCAATGTCAT+TGG	+	chr2_4:3400595-3400614	Msa0308950:CDS	30.0%
GTTTGAAGAAGGTTGAATAT+TGG	-	chr2_4:3399301-3399320	None:intergenic	30.0%
ACGAGAATTACATGAGAAAA+CGG	+	chr2_4:3399952-3399971	Msa0308950:CDS	30.0%
AACAATCAAAATGAAAGTGG+CGG	+	chr2_4:3400005-3400024	Msa0308950:CDS	30.0%
AAGAGTTGTTAACGTTGAAA+TGG	-	chr2_4:3400559-3400578	None:intergenic	30.0%
TCAACAACATTCAATGTCAT+TGG	+	chr2_4:3400595-3400614	Msa0308950:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	3399129	3400688	3399129	ID=Msa0308950;Name=Msa0308950
chr2_4	mRNA	3399129	3400688	3399129	ID=Msa0308950-mRNA-1;Parent=Msa0308950;Name=Msa0308950-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|519
chr2_4	exon	3399129	3400688	3399129	ID=Msa0308950-mRNA-1:exon:1026;Parent=Msa0308950-mRNA-1
chr2_4	CDS	3399129	3400688	3399129	ID=Msa0308950-mRNA-1:cds;Parent=Msa0308950-mRNA-1

Gene Sequence

>Msa0308950

ATGGAACTATCAGATTTACAAAACAACAAGCAGAAGAGAGGCACCACCAATATTCATCCCCATCCCCACCACCAACAACAAGAATCATCCTCACCTTCAAATTCTCATCATCAGCAGCTTGTGGTCCCTTTTGATGGAAGGCCATCACCTTTCATTATGGGCTCCATCTCCAATATTCAACCTTCTTCAAACTCTTCTCCCTCCAATTCTTCTTCTTCTTCAGCTTCAGCTTCAGCTTCTGCACCCACTCAACACCTCATTGATGCTTCCCTGGCAATTGCCACCAGATCTGAACCTCCTCTTCCAGATGTATCCAACAACAACAACAACAACCAAATTCAACCATTTTCTTCAACTTCAACCGCCGTCACCGCGGTTGCAAATCCTCCAAAACGATCCACCAAGGACAGGCACACAAAAGTTGACGGCCGTGGCCGGAGAATTCGAATGCCGGCGACATGTGCAGCAAGAGTTTTCCAGTTGACCAGAGAATTAGGCCACAAATCCGATGGTGAAACAATTGAATGGCTTCTTCAACAAGCGGAGCCGGCGATCATCGCTGCCACCGGCACCGGAACAATCCCAGCCAATTTCTCAACGCTCAACGTCTCACTCCGTAGCAGCGGCTCTACACTGTCTGCGCCGCCGTCGAAATCAGCTCCTCTCAGTTTTCATAGCGCGCTTGCACTGGCTCATCATCCTTACGAAGAAGCTGCGTACCAACACGCTCCTCTGTTAGGGTTTCATCCTCATCAACACCACCAACAACAGCAACAGCAACAACAACTGATGAACGCTGATCAAATTGGTGAAGCACTTTCAAACGAGAATTACATGAGAAAACGGTATAGAGAAGATCTTTTCAAAGATGATAACAACAATCAAAATGAAAGTGGCGGTGATGGTGATGGTTCTTCTTCTCCCAAAAGTTTCAAAACAAATCACCAACAACAGGATGATATGGGCGAAGGTGACGGTGGTGGAGGAGGTGGTGGTGGAGCTTCTTCTTCTTCTGTAGGTAATTCTTTTTCTGGACTTCTTCGACCGTCAAATATGTTACCGGCAACAGCCATGTGGGCTACAGCGGGGAGTGGAAGTACAATTTGGATGGTACCAGTGAGTGGTGGTGCTGCAACTTCTTCAAATTCAGAGACACAGACACAGACACAAAACATGTGGCCTTTTACACCTCATGTTCAGTCTCAAAATCAAGGCAACGCTGCTGCTGTAACTCCCTCTCAGTTACATTTCATGCCAAGGTTCAATGTTCCAGCTTCTGGTGGTGGTGGTGGTGGTGTTGAATTTCAAGGTGGACGTGGTGGTTTACAATTGGGTTCAGCGTATCAACCATCACAGCATTTGGGATTGGCTGTATCTGATTCCAACTTGGGTATGTTTGCTGCTCTTAATGCTTATACAAGAGCTGGCCATTTCAACGTTAACAACTCTTCTGATCATCATCATCATCAACAACATTCAATGTCATTGGACTCTCATTCTCAACCTCAACCTCAACCTAATGATGAGAGTGGAGAAGATGCTCCTAATACCTCACAATGA

Protein sequence

>Msa0308950

MELSDLQNNKQKRGTTNIHPHPHHQQQESSSPSNSHHQQLVVPFDGRPSPFIMGSISNIQPSSNSSPSNSSSSSASASASAPTQHLIDASLAIATRSEPPLPDVSNNNNNNQIQPFSSTSTAVTAVANPPKRSTKDRHTKVDGRGRRIRMPATCAARVFQLTRELGHKSDGETIEWLLQQAEPAIIAATGTGTIPANFSTLNVSLRSSGSTLSAPPSKSAPLSFHSALALAHHPYEEAAYQHAPLLGFHPHQHHQQQQQQQQLMNADQIGEALSNENYMRKRYREDLFKDDNNNQNESGGDGDGSSSPKSFKTNHQQQDDMGEGDGGGGGGGGASSSSVGNSFSGLLRPSNMLPATAMWATAGSGSTIWMVPVSGGAATSSNSETQTQTQNMWPFTPHVQSQNQGNAAAVTPSQLHFMPRFNVPASGGGGGGVEFQGGRGGLQLGSAYQPSQHLGLAVSDSNLGMFAALNAYTRAGHFNVNNSSDHHHHQQHSMSLDSHSQPQPQPNDESGEDAPNTSQ*