AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0313180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313180 MtrunA17_Chr2g0282681 91.304 138 12 0 1 138 1 138 4.81e-83 240
Msa0313180 MtrunA17_Chr4g0046611 62.238 143 40 5 3 137 4 140 3.21e-45 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATTCACTGCAGATTTC+TGG 0.165196 2_4:-8545301 Msa0313180:three_prime_UTR
CAGAATGATGAAATGGTTAT+TGG 0.234836 2_4:-8546364 Msa0313180:CDS
TTGGATTTAGTTCATATATA+TGG 0.254306 2_4:-8545217 Msa0313180:three_prime_UTR
TGTACTTCCCGAACTCATTA+AGG 0.264655 2_4:-8545536 Msa0313180:CDS
TTGATACACTGCTAAAGTTT+AGG 0.296871 2_4:-8545413 Msa0313180:CDS
TTATTAACATATTTAAAGTT+TGG 0.297150 2_4:-8545105 None:intergenic
TATATATGGTAATGGCCTTA+TGG 0.313072 2_4:-8545203 Msa0313180:three_prime_UTR
GGCCTTATGGAGTATATATA+TGG 0.321990 2_4:-8545190 Msa0313180:three_prime_UTR
TCTTCAAACCTTAATGAGTT+CGG 0.341794 2_4:+8545528 None:intergenic
CATGTCCCTCCCTTGTACTT+TGG 0.343100 2_4:+8545464 None:intergenic
CTTCAAACCTTAATGAGTTC+GGG 0.345528 2_4:+8545529 None:intergenic
CTCTCAGGTTCCTTGCAACT+TGG 0.345913 2_4:-8545236 Msa0313180:three_prime_UTR
GTATCCCTTGCACAGCTGAT+TGG 0.420642 2_4:-8545585 Msa0313180:CDS
GTCTTCTCAGAATGATGAAA+TGG 0.422507 2_4:-8546371 Msa0313180:CDS
TTATCCGTTGTAACTCTCTC+AGG 0.427679 2_4:-8545251 Msa0313180:three_prime_UTR
CACCTCAGTATCAAGGTTAG+AGG 0.432523 2_4:+8546231 None:intergenic
GATGGCATGTGCAATTGATA+TGG 0.444498 2_4:+8546277 None:intergenic
GGAACAGTAAGAAGAGTTCA+AGG 0.459894 2_4:-8545392 Msa0313180:CDS
TTAGTTCATATATATGGTAA+TGG 0.462346 2_4:-8545211 Msa0313180:three_prime_UTR
AGCCATATATATACTCCATA+AGG 0.475817 2_4:+8545188 None:intergenic
TAGCAGTGTATCAAGAAGTA+CGG 0.492333 2_4:+8545422 None:intergenic
CAGCTGATTGGACTTAGATC+AGG 0.502275 2_4:-8545573 Msa0313180:CDS
ATAATTCAGATGTTGCTCCT+AGG 0.521048 2_4:-8545324 Msa0313180:three_prime_UTR
TCTCATGACTCCAAAGTACA+AGG 0.521096 2_4:-8545474 Msa0313180:CDS
AACACAACACCTCAGTATCA+AGG 0.536128 2_4:+8546224 None:intergenic
AAGAGTTCAAGGAACAAGCT+AGG 0.536610 2_4:-8545381 Msa0313180:CDS
ATGGTTATTGGATGGCCACT+TGG 0.552969 2_4:-8546352 Msa0313180:CDS
TAAGTCCAATCAGCTGTGCA+AGG 0.574971 2_4:+8545580 None:intergenic
AGCAGTGTATCAAGAAGTAC+GGG 0.579439 2_4:+8545423 None:intergenic
TGAACTAAATCCAAGTTGCA+AGG 0.610733 2_4:+8545226 None:intergenic
AAGTCCAATCAGCTGTGCAA+GGG 0.615690 2_4:+8545581 None:intergenic
AACCTCTAACCTTGATACTG+AGG 0.629450 2_4:-8546233 Msa0313180:intron
GAATATGAGACTTAGAGTTG+TGG 0.632329 2_4:-8546317 Msa0313180:CDS
TTCAAGAAGCTAAGACCAAG+TGG 0.634705 2_4:+8546337 None:intergenic
AATGAAGAGAAACTAGTCGA+TGG 0.635220 2_4:+8546259 None:intergenic
ATTGGACTTAGATCAGGTGA+CGG 0.636987 2_4:-8545567 Msa0313180:CDS
TGACTCCAAAGTACAAGGGA+GGG 0.644573 2_4:-8545469 Msa0313180:CDS
ATGATGAAATGGTTATTGGA+TGG 0.654206 2_4:-8546360 Msa0313180:CDS
ATGACTCCAAAGTACAAGGG+AGG 0.660763 2_4:-8545470 Msa0313180:CDS
CTCATGACTCCAAAGTACAA+GGG 0.674749 2_4:-8545473 Msa0313180:CDS
GGAACCTGAGAGAGTTACAA+CGG 0.696897 2_4:+8545247 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAATTCTAAGAAAAAT+AGG + chr2_4:8546336-8546355 None:intergenic 10.0%
!!! TATTTTTCTTAGAATTTAAT+TGG - chr2_4:8546335-8546354 Msa0313180:CDS 10.0%
!!! AATTAAATTCTAAGAAAAAT+AGG + chr2_4:8546336-8546355 None:intergenic 10.0%
!!! TATTTTTCTTAGAATTTAAT+TGG - chr2_4:8546335-8546354 Msa0313180:CDS 10.0%
!! AAAAAACAAGAAATTAGTGA+AGG - chr2_4:8545975-8545994 Msa0313180:intron 20.0%
!! AAAAACAAGAAATTAGTGAA+GGG - chr2_4:8545976-8545995 Msa0313180:intron 20.0%
!!! TTGGATTTAGTTCATATATA+TGG - chr2_4:8546263-8546282 Msa0313180:CDS 20.0%
!! TTAGTTCATATATATGGTAA+TGG - chr2_4:8546269-8546288 Msa0313180:CDS 20.0%
!! AAAAAACAAGAAATTAGTGA+AGG - chr2_4:8545975-8545994 Msa0313180:intron 20.0%
!! AAAAACAAGAAATTAGTGAA+GGG - chr2_4:8545976-8545995 Msa0313180:intron 20.0%
!!! TTGGATTTAGTTCATATATA+TGG - chr2_4:8546263-8546282 Msa0313180:CDS 20.0%
!! TTAGTTCATATATATGGTAA+TGG - chr2_4:8546269-8546288 Msa0313180:CDS 20.0%
!! ACTAAGTGGTATTCATTTTA+TGG + chr2_4:8545323-8545342 None:intergenic 25.0%
! ATAGTGTTTATGTTACTAAG+TGG + chr2_4:8545337-8545356 None:intergenic 25.0%
! AGTGACTATTAACAACTTAT+AGG - chr2_4:8545418-8545437 Msa0313180:CDS 25.0%
! TTGTTGAATTGACAATTATC+AGG + chr2_4:8545572-8545591 None:intergenic 25.0%
! TTGTCAATTCAACAAAAACA+AGG - chr2_4:8545577-8545596 Msa0313180:CDS 25.0%
! AAGTAGAATAAATTGTAAGC+AGG + chr2_4:8545823-8545842 None:intergenic 25.0%
! CTAAGAAAAATAGGTCAAAA+AGG + chr2_4:8546327-8546346 None:intergenic 25.0%
!! ACTAAGTGGTATTCATTTTA+TGG + chr2_4:8545323-8545342 None:intergenic 25.0%
! ATAGTGTTTATGTTACTAAG+TGG + chr2_4:8545337-8545356 None:intergenic 25.0%
! AGTGACTATTAACAACTTAT+AGG - chr2_4:8545418-8545437 Msa0313180:CDS 25.0%
! TTGTTGAATTGACAATTATC+AGG + chr2_4:8545572-8545591 None:intergenic 25.0%
! TTGTCAATTCAACAAAAACA+AGG - chr2_4:8545577-8545596 Msa0313180:CDS 25.0%
! AAGTAGAATAAATTGTAAGC+AGG + chr2_4:8545823-8545842 None:intergenic 25.0%
! CTAAGAAAAATAGGTCAAAA+AGG + chr2_4:8546327-8546346 None:intergenic 25.0%
CAGAATGATGAAATGGTTAT+TGG - chr2_4:8545116-8545135 Msa0313180:three_prime_UTR 30.0%
ATGATGAAATGGTTATTGGA+TGG - chr2_4:8545120-8545139 Msa0313180:three_prime_UTR 30.0%
TCTTATTCCTTTCTCAACTT+TGG - chr2_4:8545278-8545297 Msa0313180:three_prime_UTR 30.0%
GACTATTAACAACTTATAGG+CGG - chr2_4:8545421-8545440 Msa0313180:CDS 30.0%
ACTATTAACAACTTATAGGC+GGG - chr2_4:8545422-8545441 Msa0313180:CDS 30.0%
TCTTCAAACCTTAATGAGTT+CGG + chr2_4:8545955-8545974 None:intergenic 30.0%
TTGATACACTGCTAAAGTTT+AGG - chr2_4:8546067-8546086 Msa0313180:intron 30.0%
!! TATATATGGTAATGGCCTTA+TGG - chr2_4:8546277-8546296 Msa0313180:CDS 30.0%
AGCCATATATATACTCCATA+AGG + chr2_4:8546295-8546314 None:intergenic 30.0%
CAGAATGATGAAATGGTTAT+TGG - chr2_4:8545116-8545135 Msa0313180:three_prime_UTR 30.0%
ATGATGAAATGGTTATTGGA+TGG - chr2_4:8545120-8545139 Msa0313180:three_prime_UTR 30.0%
TCTTATTCCTTTCTCAACTT+TGG - chr2_4:8545278-8545297 Msa0313180:three_prime_UTR 30.0%
GACTATTAACAACTTATAGG+CGG - chr2_4:8545421-8545440 Msa0313180:CDS 30.0%
ACTATTAACAACTTATAGGC+GGG - chr2_4:8545422-8545441 Msa0313180:CDS 30.0%
TCTTCAAACCTTAATGAGTT+CGG + chr2_4:8545955-8545974 None:intergenic 30.0%
TTGATACACTGCTAAAGTTT+AGG - chr2_4:8546067-8546086 Msa0313180:intron 30.0%
!! TATATATGGTAATGGCCTTA+TGG - chr2_4:8546277-8546296 Msa0313180:CDS 30.0%
AGCCATATATATACTCCATA+AGG + chr2_4:8546295-8546314 None:intergenic 30.0%
GTCTTCTCAGAATGATGAAA+TGG - chr2_4:8545109-8545128 Msa0313180:three_prime_UTR 35.0%
GAATATGAGACTTAGAGTTG+TGG - chr2_4:8545163-8545182 Msa0313180:three_prime_UTR 35.0%
AATGAAGAGAAACTAGTCGA+TGG + chr2_4:8545224-8545243 None:intergenic 35.0%
TGTCAAACCAAAGTTGAGAA+AGG + chr2_4:8545288-8545307 None:intergenic 35.0%
CTTTACAAGTACATTTGCCT+TGG + chr2_4:8545400-8545419 None:intergenic 35.0%
!!! TTTTAAGTACCGATCCATTG+TGG + chr2_4:8545460-8545479 None:intergenic 35.0%
!! TGTTGGTTCGACATAAACAA+TGG + chr2_4:8545626-8545645 None:intergenic 35.0%
TCTCATCCATAAAGAGAAAG+AGG - chr2_4:8545661-8545680 Msa0313180:intron 35.0%
! CTTCTGAAAGTAGAAGATCA+CGG + chr2_4:8545688-8545707 None:intergenic 35.0%
! GATTTTCAAATACCCGTCTA+TGG - chr2_4:8545738-8545757 Msa0313180:intron 35.0%
CTTCAAACCTTAATGAGTTC+GGG + chr2_4:8545954-8545973 None:intergenic 35.0%
TAGCAGTGTATCAAGAAGTA+CGG + chr2_4:8546061-8546080 None:intergenic 35.0%
ATAATTCAGATGTTGCTCCT+AGG - chr2_4:8546156-8546175 Msa0313180:intron 35.0%
CAAAATTCACTGCAGATTTC+TGG - chr2_4:8546179-8546198 Msa0313180:intron 35.0%
TGAACTAAATCCAAGTTGCA+AGG + chr2_4:8546257-8546276 None:intergenic 35.0%
! GGCCTTATGGAGTATATATA+TGG - chr2_4:8546290-8546309 Msa0313180:CDS 35.0%
GTCTTCTCAGAATGATGAAA+TGG - chr2_4:8545109-8545128 Msa0313180:three_prime_UTR 35.0%
GAATATGAGACTTAGAGTTG+TGG - chr2_4:8545163-8545182 Msa0313180:three_prime_UTR 35.0%
AATGAAGAGAAACTAGTCGA+TGG + chr2_4:8545224-8545243 None:intergenic 35.0%
TGTCAAACCAAAGTTGAGAA+AGG + chr2_4:8545288-8545307 None:intergenic 35.0%
CTTTACAAGTACATTTGCCT+TGG + chr2_4:8545400-8545419 None:intergenic 35.0%
!!! TTTTAAGTACCGATCCATTG+TGG + chr2_4:8545460-8545479 None:intergenic 35.0%
!! TGTTGGTTCGACATAAACAA+TGG + chr2_4:8545626-8545645 None:intergenic 35.0%
TCTCATCCATAAAGAGAAAG+AGG - chr2_4:8545661-8545680 Msa0313180:intron 35.0%
! CTTCTGAAAGTAGAAGATCA+CGG + chr2_4:8545688-8545707 None:intergenic 35.0%
! GATTTTCAAATACCCGTCTA+TGG - chr2_4:8545738-8545757 Msa0313180:intron 35.0%
CTTCAAACCTTAATGAGTTC+GGG + chr2_4:8545954-8545973 None:intergenic 35.0%
TAGCAGTGTATCAAGAAGTA+CGG + chr2_4:8546061-8546080 None:intergenic 35.0%
ATAATTCAGATGTTGCTCCT+AGG - chr2_4:8546156-8546175 Msa0313180:intron 35.0%
CAAAATTCACTGCAGATTTC+TGG - chr2_4:8546179-8546198 Msa0313180:intron 35.0%
TGAACTAAATCCAAGTTGCA+AGG + chr2_4:8546257-8546276 None:intergenic 35.0%
! GGCCTTATGGAGTATATATA+TGG - chr2_4:8546290-8546309 Msa0313180:CDS 35.0%
TTCAAGAAGCTAAGACCAAG+TGG + chr2_4:8545146-8545165 None:intergenic 40.0%
GATGGCATGTGCAATTGATA+TGG + chr2_4:8545206-8545225 None:intergenic 40.0%
AACCTCTAACCTTGATACTG+AGG - chr2_4:8545247-8545266 Msa0313180:three_prime_UTR 40.0%
AACACAACACCTCAGTATCA+AGG + chr2_4:8545259-8545278 None:intergenic 40.0%
TACTATGGACCACAATGGAT+CGG - chr2_4:8545448-8545467 Msa0313180:CDS 40.0%
TCTCAGTGGTAAGAATAAGG+AGG + chr2_4:8545543-8545562 None:intergenic 40.0%
TCGTCTCAGTGGTAAGAATA+AGG + chr2_4:8545546-8545565 None:intergenic 40.0%
GACAATTACCTGATTGGTGT+TGG + chr2_4:8545643-8545662 None:intergenic 40.0%
GGATGAGACAATTACCTGAT+TGG + chr2_4:8545649-8545668 None:intergenic 40.0%
CGTGATCTTCTACTTTCAGA+AGG - chr2_4:8545686-8545705 Msa0313180:intron 40.0%
TTTCAAATACCCGTCTATGG+TGG - chr2_4:8545741-8545760 Msa0313180:intron 40.0%
! TTTGAAGTCTCCACCATAGA+CGG + chr2_4:8545754-8545773 None:intergenic 40.0%
TGAAGAAAGATGCTGTAGAC+TGG + chr2_4:8545864-8545883 None:intergenic 40.0%
ATTGGACTTAGATCAGGTGA+CGG - chr2_4:8545913-8545932 Msa0313180:intron 40.0%
TGTACTTCCCGAACTCATTA+AGG - chr2_4:8545944-8545963 Msa0313180:intron 40.0%
TCTCATGACTCCAAAGTACA+AGG - chr2_4:8546006-8546025 Msa0313180:intron 40.0%
CTCATGACTCCAAAGTACAA+GGG - chr2_4:8546007-8546026 Msa0313180:intron 40.0%
! AGCAGTGTATCAAGAAGTAC+GGG + chr2_4:8546060-8546079 None:intergenic 40.0%
GGAACAGTAAGAAGAGTTCA+AGG - chr2_4:8546088-8546107 Msa0313180:intron 40.0%
AAGAGTTCAAGGAACAAGCT+AGG - chr2_4:8546099-8546118 Msa0313180:intron 40.0%
! ATCTGCAGTGAATTTTGCCT+AGG + chr2_4:8546176-8546195 None:intergenic 40.0%
TTATCCGTTGTAACTCTCTC+AGG - chr2_4:8546229-8546248 Msa0313180:intron 40.0%
TTCAAGAAGCTAAGACCAAG+TGG + chr2_4:8545146-8545165 None:intergenic 40.0%
GATGGCATGTGCAATTGATA+TGG + chr2_4:8545206-8545225 None:intergenic 40.0%
AACCTCTAACCTTGATACTG+AGG - chr2_4:8545247-8545266 Msa0313180:three_prime_UTR 40.0%
AACACAACACCTCAGTATCA+AGG + chr2_4:8545259-8545278 None:intergenic 40.0%
TACTATGGACCACAATGGAT+CGG - chr2_4:8545448-8545467 Msa0313180:CDS 40.0%
TCTCAGTGGTAAGAATAAGG+AGG + chr2_4:8545543-8545562 None:intergenic 40.0%
TCGTCTCAGTGGTAAGAATA+AGG + chr2_4:8545546-8545565 None:intergenic 40.0%
GACAATTACCTGATTGGTGT+TGG + chr2_4:8545643-8545662 None:intergenic 40.0%
GGATGAGACAATTACCTGAT+TGG + chr2_4:8545649-8545668 None:intergenic 40.0%
CGTGATCTTCTACTTTCAGA+AGG - chr2_4:8545686-8545705 Msa0313180:intron 40.0%
TTTCAAATACCCGTCTATGG+TGG - chr2_4:8545741-8545760 Msa0313180:intron 40.0%
! TTTGAAGTCTCCACCATAGA+CGG + chr2_4:8545754-8545773 None:intergenic 40.0%
TGAAGAAAGATGCTGTAGAC+TGG + chr2_4:8545864-8545883 None:intergenic 40.0%
ATTGGACTTAGATCAGGTGA+CGG - chr2_4:8545913-8545932 Msa0313180:intron 40.0%
TGTACTTCCCGAACTCATTA+AGG - chr2_4:8545944-8545963 Msa0313180:intron 40.0%
TCTCATGACTCCAAAGTACA+AGG - chr2_4:8546006-8546025 Msa0313180:intron 40.0%
CTCATGACTCCAAAGTACAA+GGG - chr2_4:8546007-8546026 Msa0313180:intron 40.0%
! AGCAGTGTATCAAGAAGTAC+GGG + chr2_4:8546060-8546079 None:intergenic 40.0%
GGAACAGTAAGAAGAGTTCA+AGG - chr2_4:8546088-8546107 Msa0313180:intron 40.0%
AAGAGTTCAAGGAACAAGCT+AGG - chr2_4:8546099-8546118 Msa0313180:intron 40.0%
! ATCTGCAGTGAATTTTGCCT+AGG + chr2_4:8546176-8546195 None:intergenic 40.0%
TTATCCGTTGTAACTCTCTC+AGG - chr2_4:8546229-8546248 Msa0313180:intron 40.0%
ATGGTTATTGGATGGCCACT+TGG - chr2_4:8545128-8545147 Msa0313180:three_prime_UTR 45.0%
CACCTCAGTATCAAGGTTAG+AGG + chr2_4:8545252-8545271 None:intergenic 45.0%
GTTTCACCTTCAACTGTCCA+AGG - chr2_4:8545380-8545399 Msa0313180:CDS 45.0%
CATTTGCCTTGGACAGTTGA+AGG + chr2_4:8545389-8545408 None:intergenic 45.0%
! CTTATAGGCGGGTTCTACTA+TGG - chr2_4:8545433-8545452 Msa0313180:CDS 45.0%
GGTTCTACTATGGACCACAA+TGG - chr2_4:8545443-8545462 Msa0313180:CDS 45.0%
TTATCAGGTCATCGTCTCAG+TGG + chr2_4:8545557-8545576 None:intergenic 45.0%
ATGTCGAACCAACACCAATC+AGG - chr2_4:8545632-8545651 Msa0313180:intron 45.0%
CACGGTCCTCTTTCTCTTTA+TGG + chr2_4:8545670-8545689 None:intergenic 45.0%
TTGAAGTCTCCACCATAGAC+GGG + chr2_4:8545753-8545772 None:intergenic 45.0%
AAGTCCAATCAGCTGTGCAA+GGG + chr2_4:8545902-8545921 None:intergenic 45.0%
TAAGTCCAATCAGCTGTGCA+AGG + chr2_4:8545903-8545922 None:intergenic 45.0%
CAGCTGATTGGACTTAGATC+AGG - chr2_4:8545907-8545926 Msa0313180:intron 45.0%
ATGACTCCAAAGTACAAGGG+AGG - chr2_4:8546010-8546029 Msa0313180:intron 45.0%
TGACTCCAAAGTACAAGGGA+GGG - chr2_4:8546011-8546030 Msa0313180:intron 45.0%
GGAACCTGAGAGAGTTACAA+CGG + chr2_4:8546236-8546255 None:intergenic 45.0%
ATGGTTATTGGATGGCCACT+TGG - chr2_4:8545128-8545147 Msa0313180:three_prime_UTR 45.0%
CACCTCAGTATCAAGGTTAG+AGG + chr2_4:8545252-8545271 None:intergenic 45.0%
GTTTCACCTTCAACTGTCCA+AGG - chr2_4:8545380-8545399 Msa0313180:CDS 45.0%
CATTTGCCTTGGACAGTTGA+AGG + chr2_4:8545389-8545408 None:intergenic 45.0%
! CTTATAGGCGGGTTCTACTA+TGG - chr2_4:8545433-8545452 Msa0313180:CDS 45.0%
GGTTCTACTATGGACCACAA+TGG - chr2_4:8545443-8545462 Msa0313180:CDS 45.0%
TTATCAGGTCATCGTCTCAG+TGG + chr2_4:8545557-8545576 None:intergenic 45.0%
ATGTCGAACCAACACCAATC+AGG - chr2_4:8545632-8545651 Msa0313180:intron 45.0%
CACGGTCCTCTTTCTCTTTA+TGG + chr2_4:8545670-8545689 None:intergenic 45.0%
TTGAAGTCTCCACCATAGAC+GGG + chr2_4:8545753-8545772 None:intergenic 45.0%
AAGTCCAATCAGCTGTGCAA+GGG + chr2_4:8545902-8545921 None:intergenic 45.0%
TAAGTCCAATCAGCTGTGCA+AGG + chr2_4:8545903-8545922 None:intergenic 45.0%
CAGCTGATTGGACTTAGATC+AGG - chr2_4:8545907-8545926 Msa0313180:intron 45.0%
ATGACTCCAAAGTACAAGGG+AGG - chr2_4:8546010-8546029 Msa0313180:intron 45.0%
TGACTCCAAAGTACAAGGGA+GGG - chr2_4:8546011-8546030 Msa0313180:intron 45.0%
GGAACCTGAGAGAGTTACAA+CGG + chr2_4:8546236-8546255 None:intergenic 45.0%
GTATCCCTTGCACAGCTGAT+TGG - chr2_4:8545895-8545914 Msa0313180:intron 50.0%
CATGTCCCTCCCTTGTACTT+TGG + chr2_4:8546019-8546038 None:intergenic 50.0%
CTCTCAGGTTCCTTGCAACT+TGG - chr2_4:8546244-8546263 Msa0313180:CDS 50.0%
GTATCCCTTGCACAGCTGAT+TGG - chr2_4:8545895-8545914 Msa0313180:intron 50.0%
CATGTCCCTCCCTTGTACTT+TGG + chr2_4:8546019-8546038 None:intergenic 50.0%
CTCTCAGGTTCCTTGCAACT+TGG - chr2_4:8546244-8546263 Msa0313180:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 8545107 8546395 8545107 ID=Msa0313180;Name=Msa0313180
chr2_4 mRNA 8545107 8546395 8545107 ID=Msa0313180-mRNA-1;Parent=Msa0313180;Name=Msa0313180-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|1|0.5|1|1|1|2|270|140
chr2_4 exon 8545107 8545637 8545107 ID=Msa0313180-mRNA-1:exon:3251;Parent=Msa0313180-mRNA-1
chr2_4 exon 8546234 8546395 8546234 ID=Msa0313180-mRNA-1:exon:3250;Parent=Msa0313180-mRNA-1
chr2_4 CDS 8546234 8546395 8546234 ID=Msa0313180-mRNA-1:cds;Parent=Msa0313180-mRNA-1
chr2_4 CDS 8545377 8545637 8545377 ID=Msa0313180-mRNA-1:cds;Parent=Msa0313180-mRNA-1
chr2_4 three_prime_UTR 8545107 8545376 8545107 ID=Msa0313180-mRNA-1:three_prime_utr;Parent=Msa0313180-mRNA-1
Gene Sequence

>Msa0313180

ATGTCTTCTCAGAATGATGAAATGGTTATTGGATGGCCACTTGGTCTTAGCTTCTTGAATATGAGACTTAGAGTTGTGGAAGCTTCTTCAGTGAAACCATATCAATTGCACATGCCATCGACTAGTTTCTCTTCATTTTCAACCTCTAACCTTGATACTGAGTCTACAGCATCTTTCTTCAAAGACAACAGTGTATCCCTTGCACAGCTGATTGGACTTAGATCAGGTGACGGAGAACACGTGTACTTCCCGAACTCATTAAGGTTTGAAGAAAAAAACAAGAAATTAGTGAAGGGTTCTTGTTCTCATGACTCCAAAGTACAAGGGAGGGACATGTCTAGAGTCATATGCATACCCGTACTTCTTGATACACTGCTAAAGTTTAGGAACAGTAAGAAGAGTTCAAGGAACAAGCTAGGATGA
Protein sequence

>Msa0313180

MSSQNDEMVIGWPLGLSFLNMRLRVVEASSVKPYQLHMPSTSFSSFSTSNLDTESTASFFKDNSVSLAQLIGLRSGDGEHVYFPNSLRFEEKNKKLVKGSCSHDSKVQGRDMSRVICIPVLLDTLLKFRNSKKSSRNKLG*