AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0313190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313190 MtrunA17_Chr2g0282691 94.697 264 13 1 1 264 1 263 1.37e-109 317
Msa0313190 MtrunA17_Chr4g0046601 55.556 135 50 4 130 261 230 357 8.05e-22 93.2
Msa0313190 MtrunA17_Chr4g0046601 43.165 139 65 4 1 131 1 133 9.89e-19 84.3
Msa0313190 MtrunA17_Chr2g0324881 30.859 256 116 8 21 254 21 237 5.17e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313190 AT2G33850.1 31.429 210 101 7 21 202 22 216 1.39e-11 63.5

Find 51 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTGGTTTGTTTGTAAC+TGG 0.131657 2_4:+8561297 None:intergenic
TGTAGTTTCCATGATTATTC+TGG 0.252931 2_4:+8560731 None:intergenic
TTGTGTCCCTCAACTCATTT+TGG 0.256754 2_4:+8561073 None:intergenic
CAAAATAGCTATGGCTTATA+TGG 0.260837 2_4:-8561249 Msa0313190:CDS
GTTATAGTATTTGTTGTTGT+TGG 0.293134 2_4:+8561146 None:intergenic
TATTACAACAAAGACACTTT+TGG 0.320337 2_4:-8561102 Msa0313190:CDS
AACTGGGGATTCTTCTTTGT+TGG 0.334551 2_4:+8561314 None:intergenic
TTATAGTATTTGTTGTTGTT+GGG 0.334833 2_4:+8561147 None:intergenic
ATGAAAGGTGGTTGCTCCTC+TGG 0.339057 2_4:+8561282 None:intergenic
ATTATAATGCTCAGAATAAC+AGG 0.351179 2_4:-8560930 Msa0313190:CDS
GGTGGATGCCGTGGCGGCGG+TGG 0.353742 2_4:+8561203 None:intergenic
ACTGGGGATTCTTCTTTGTT+GGG 0.370013 2_4:+8561315 None:intergenic
CCTCTGGTTTGTTTGTAACT+GGG 0.383487 2_4:+8561298 None:intergenic
TACAACAATAATAATAACTA+TGG 0.387734 2_4:-8560769 Msa0313190:CDS
GATGAATCACCATACATTGT+TGG 0.414049 2_4:+8560820 None:intergenic
GCTGAAGAATTCACTCTCTC+TGG 0.421980 2_4:+8561371 None:intergenic
GAGTGAACAACAATAATAAC+TGG 0.430385 2_4:-8560792 Msa0313190:CDS
TGTGTCTTCAAAGTTAGAAT+TGG 0.437689 2_4:+8561179 None:intergenic
AAATACAATCCAACAATGTA+TGG 0.452288 2_4:-8560829 Msa0313190:CDS
TAAGAGGATGAAAGAAATGT+AGG 0.456040 2_4:+8561425 None:intergenic
GATGCCGTGGCGGCGGTGGA+AGG 0.457928 2_4:+8561207 None:intergenic
GGTGGTGGATGCCGTGGCGG+CGG 0.460270 2_4:+8561200 None:intergenic
CTCTGGTTTGTTTGTAACTG+GGG 0.464914 2_4:+8561299 None:intergenic
AATGAGTACCAGAATAATCA+TGG 0.478186 2_4:-8560739 Msa0313190:CDS
GTCTTCAAAGTTAGAATTGG+TGG 0.482976 2_4:+8561182 None:intergenic
TGTTGTTGAAGTAGTACTCT+TGG 0.484835 2_4:+8561001 None:intergenic
GCAACAAAAGAGTTGTTAAG+AGG 0.489505 2_4:+8561409 None:intergenic
AGGATGAAAGAAATGTAGGT+TGG 0.497294 2_4:+8561429 None:intergenic
GGATGAAAGAAATGTAGGTT+GGG 0.498004 2_4:+8561430 None:intergenic
TATAGTATTTGTTGTTGTTG+GGG 0.516144 2_4:+8561148 None:intergenic
AAATAACCAAAATGAGTTGA+GGG 0.518734 2_4:-8561079 Msa0313190:CDS
TTACAAGAACCAAGAGGAGT+TGG 0.528023 2_4:-8560704 Msa0313190:CDS
GAAATAACCAAAATGAGTTG+AGG 0.542156 2_4:-8561080 Msa0313190:CDS
GACACAAAGTACAATGAAGA+AGG 0.550520 2_4:-8561057 Msa0313190:CDS
CCTCAGACACAAAATAGCTA+TGG 0.562030 2_4:-8561258 Msa0313190:CDS
CATTCCTTCCACCGCCGCCA+CGG 0.564976 2_4:-8561211 Msa0313190:CDS
AGAAGGTTACAACTCCATGA+TGG 0.580265 2_4:-8561040 Msa0313190:CDS
AGGTACAATGGTGAGAAACA+AGG 0.586625 2_4:-8560910 Msa0313190:CDS
TTCAAAGTTAGAATTGGTGG+TGG 0.587007 2_4:+8561185 None:intergenic
ATGTATGGTGATTCATCTAG+AGG 0.598645 2_4:-8560814 Msa0313190:CDS
TGTGTCTGAGGAATGAAAGG+TGG 0.603943 2_4:+8561270 None:intergenic
AATAATCATGGAAACTACAA+TGG 0.611623 2_4:-8560727 Msa0313190:CDS
GCTCAGAATAACAGGTACAA+TGG 0.619912 2_4:-8560922 Msa0313190:CDS
AAGGAATGTGATCAGAGTCA+TGG 0.626750 2_4:+8561226 None:intergenic
AGAATTGGTGGTGGATGCCG+TGG 0.633457 2_4:+8561194 None:intergenic
ATTGGTGGTGGATGCCGTGG+CGG 0.636866 2_4:+8561197 None:intergenic
GGTACAATGGTGAGAAACAA+GGG 0.647160 2_4:-8560909 Msa0313190:CDS
AGAAATGTAGGTTGGGAATG+TGG 0.649922 2_4:+8561437 None:intergenic
TCTCTGGCTATAGTTTGTAG+AGG 0.668021 2_4:+8561387 None:intergenic
CCCAGTTACAAACAAACCAG+AGG 0.679700 2_4:-8561298 Msa0313190:CDS
CAATGGTTACAAGAACCAAG+AGG 0.723148 2_4:-8560710 Msa0313190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAACAATAATAATAACTA+TGG - chr2_4:8561341-8561360 Msa0313190:CDS 15.0%
!! TACAACAATAATAATAACTA+TGG - chr2_4:8561341-8561360 Msa0313190:CDS 15.0%
!!! TTATAGTATTTGTTGTTGTT+GGG + chr2_4:8560966-8560985 None:intergenic 20.0%
!!! TTATAGTATTTGTTGTTGTT+GGG + chr2_4:8560966-8560985 None:intergenic 20.0%
!!! TATAGTATTTGTTGTTGTTG+GGG + chr2_4:8560965-8560984 None:intergenic 25.0%
!!! GTTATAGTATTTGTTGTTGT+TGG + chr2_4:8560967-8560986 None:intergenic 25.0%
!! TATTACAACAAAGACACTTT+TGG - chr2_4:8561008-8561027 Msa0313190:CDS 25.0%
!! AAATAACCAAAATGAGTTGA+GGG - chr2_4:8561031-8561050 Msa0313190:CDS 25.0%
! ATTATAATGCTCAGAATAAC+AGG - chr2_4:8561180-8561199 Msa0313190:CDS 25.0%
! AAATACAATCCAACAATGTA+TGG - chr2_4:8561281-8561300 Msa0313190:CDS 25.0%
! AATAATCATGGAAACTACAA+TGG - chr2_4:8561383-8561402 Msa0313190:CDS 25.0%
!!! TATAGTATTTGTTGTTGTTG+GGG + chr2_4:8560965-8560984 None:intergenic 25.0%
!!! GTTATAGTATTTGTTGTTGT+TGG + chr2_4:8560967-8560986 None:intergenic 25.0%
!! TATTACAACAAAGACACTTT+TGG - chr2_4:8561008-8561027 Msa0313190:CDS 25.0%
!! AAATAACCAAAATGAGTTGA+GGG - chr2_4:8561031-8561050 Msa0313190:CDS 25.0%
! ATTATAATGCTCAGAATAAC+AGG - chr2_4:8561180-8561199 Msa0313190:CDS 25.0%
! AAATACAATCCAACAATGTA+TGG - chr2_4:8561281-8561300 Msa0313190:CDS 25.0%
! AATAATCATGGAAACTACAA+TGG - chr2_4:8561383-8561402 Msa0313190:CDS 25.0%
TAAGAGGATGAAAGAAATGT+AGG + chr2_4:8560688-8560707 None:intergenic 30.0%
CAAAATAGCTATGGCTTATA+TGG - chr2_4:8560861-8560880 Msa0313190:CDS 30.0%
TGTGTCTTCAAAGTTAGAAT+TGG + chr2_4:8560934-8560953 None:intergenic 30.0%
GAAATAACCAAAATGAGTTG+AGG - chr2_4:8561030-8561049 Msa0313190:CDS 30.0%
!!! GTTGTTTTGTTTTTCCATCA+TGG + chr2_4:8561087-8561106 None:intergenic 30.0%
GAGTGAACAACAATAATAAC+TGG - chr2_4:8561318-8561337 Msa0313190:CDS 30.0%
AATGAGTACCAGAATAATCA+TGG - chr2_4:8561371-8561390 Msa0313190:CDS 30.0%
TGTAGTTTCCATGATTATTC+TGG + chr2_4:8561382-8561401 None:intergenic 30.0%
TAAGAGGATGAAAGAAATGT+AGG + chr2_4:8560688-8560707 None:intergenic 30.0%
CAAAATAGCTATGGCTTATA+TGG - chr2_4:8560861-8560880 Msa0313190:CDS 30.0%
TGTGTCTTCAAAGTTAGAAT+TGG + chr2_4:8560934-8560953 None:intergenic 30.0%
GAAATAACCAAAATGAGTTG+AGG - chr2_4:8561030-8561049 Msa0313190:CDS 30.0%
!!! GTTGTTTTGTTTTTCCATCA+TGG + chr2_4:8561087-8561106 None:intergenic 30.0%
GAGTGAACAACAATAATAAC+TGG - chr2_4:8561318-8561337 Msa0313190:CDS 30.0%
AATGAGTACCAGAATAATCA+TGG - chr2_4:8561371-8561390 Msa0313190:CDS 30.0%
TGTAGTTTCCATGATTATTC+TGG + chr2_4:8561382-8561401 None:intergenic 30.0%
GGATGAAAGAAATGTAGGTT+GGG + chr2_4:8560683-8560702 None:intergenic 35.0%
AGGATGAAAGAAATGTAGGT+TGG + chr2_4:8560684-8560703 None:intergenic 35.0%
GCAACAAAAGAGTTGTTAAG+AGG + chr2_4:8560704-8560723 None:intergenic 35.0%
! TTTTGTGTCTGAGGAATGAA+AGG + chr2_4:8560846-8560865 None:intergenic 35.0%
TTCAAAGTTAGAATTGGTGG+TGG + chr2_4:8560928-8560947 None:intergenic 35.0%
GTCTTCAAAGTTAGAATTGG+TGG + chr2_4:8560931-8560950 None:intergenic 35.0%
GACACAAAGTACAATGAAGA+AGG - chr2_4:8561053-8561072 Msa0313190:CDS 35.0%
TGTTGTTGAAGTAGTACTCT+TGG + chr2_4:8561112-8561131 None:intergenic 35.0%
! GATGAGTGACACAAGATTTT+TGG - chr2_4:8561223-8561242 Msa0313190:CDS 35.0%
!! AGTGACACAAGATTTTTGGA+AGG - chr2_4:8561227-8561246 Msa0313190:CDS 35.0%
GATGAATCACCATACATTGT+TGG + chr2_4:8561293-8561312 None:intergenic 35.0%
ATGTATGGTGATTCATCTAG+AGG - chr2_4:8561296-8561315 Msa0313190:CDS 35.0%
GGATGAAAGAAATGTAGGTT+GGG + chr2_4:8560683-8560702 None:intergenic 35.0%
AGGATGAAAGAAATGTAGGT+TGG + chr2_4:8560684-8560703 None:intergenic 35.0%
GCAACAAAAGAGTTGTTAAG+AGG + chr2_4:8560704-8560723 None:intergenic 35.0%
! TTTTGTGTCTGAGGAATGAA+AGG + chr2_4:8560846-8560865 None:intergenic 35.0%
TTCAAAGTTAGAATTGGTGG+TGG + chr2_4:8560928-8560947 None:intergenic 35.0%
GTCTTCAAAGTTAGAATTGG+TGG + chr2_4:8560931-8560950 None:intergenic 35.0%
GACACAAAGTACAATGAAGA+AGG - chr2_4:8561053-8561072 Msa0313190:CDS 35.0%
TGTTGTTGAAGTAGTACTCT+TGG + chr2_4:8561112-8561131 None:intergenic 35.0%
! GATGAGTGACACAAGATTTT+TGG - chr2_4:8561223-8561242 Msa0313190:CDS 35.0%
!! AGTGACACAAGATTTTTGGA+AGG - chr2_4:8561227-8561246 Msa0313190:CDS 35.0%
GATGAATCACCATACATTGT+TGG + chr2_4:8561293-8561312 None:intergenic 35.0%
ATGTATGGTGATTCATCTAG+AGG - chr2_4:8561296-8561315 Msa0313190:CDS 35.0%
! AGAAATGTAGGTTGGGAATG+TGG + chr2_4:8560676-8560695 None:intergenic 40.0%
! TCTCTGGCTATAGTTTGTAG+AGG + chr2_4:8560726-8560745 None:intergenic 40.0%
ACTGGGGATTCTTCTTTGTT+GGG + chr2_4:8560798-8560817 None:intergenic 40.0%
AACTGGGGATTCTTCTTTGT+TGG + chr2_4:8560799-8560818 None:intergenic 40.0%
!! CTCTGGTTTGTTTGTAACTG+GGG + chr2_4:8560814-8560833 None:intergenic 40.0%
!! CCTCTGGTTTGTTTGTAACT+GGG + chr2_4:8560815-8560834 None:intergenic 40.0%
!! TCCTCTGGTTTGTTTGTAAC+TGG + chr2_4:8560816-8560835 None:intergenic 40.0%
!! CCATAGCTATTTTGTGTCTG+AGG + chr2_4:8560855-8560874 None:intergenic 40.0%
CCTCAGACACAAAATAGCTA+TGG - chr2_4:8560852-8560871 Msa0313190:CDS 40.0%
AAGGAATGTGATCAGAGTCA+TGG + chr2_4:8560887-8560906 None:intergenic 40.0%
! TTGTGTCCCTCAACTCATTT+TGG + chr2_4:8561040-8561059 None:intergenic 40.0%
AGAAGGTTACAACTCCATGA+TGG - chr2_4:8561070-8561089 Msa0313190:CDS 40.0%
GCTCAGAATAACAGGTACAA+TGG - chr2_4:8561188-8561207 Msa0313190:CDS 40.0%
AGGTACAATGGTGAGAAACA+AGG - chr2_4:8561200-8561219 Msa0313190:CDS 40.0%
GGTACAATGGTGAGAAACAA+GGG - chr2_4:8561201-8561220 Msa0313190:CDS 40.0%
!! GACACAAGATTTTTGGAAGG+TGG - chr2_4:8561230-8561249 Msa0313190:CDS 40.0%
CAATGGTTACAAGAACCAAG+AGG - chr2_4:8561400-8561419 Msa0313190:CDS 40.0%
TTACAAGAACCAAGAGGAGT+TGG - chr2_4:8561406-8561425 Msa0313190:CDS 40.0%
! GTTCATTTTCCAACTCCTCT+TGG + chr2_4:8561418-8561437 None:intergenic 40.0%
! AGAAATGTAGGTTGGGAATG+TGG + chr2_4:8560676-8560695 None:intergenic 40.0%
! TCTCTGGCTATAGTTTGTAG+AGG + chr2_4:8560726-8560745 None:intergenic 40.0%
ACTGGGGATTCTTCTTTGTT+GGG + chr2_4:8560798-8560817 None:intergenic 40.0%
AACTGGGGATTCTTCTTTGT+TGG + chr2_4:8560799-8560818 None:intergenic 40.0%
!! CTCTGGTTTGTTTGTAACTG+GGG + chr2_4:8560814-8560833 None:intergenic 40.0%
!! CCTCTGGTTTGTTTGTAACT+GGG + chr2_4:8560815-8560834 None:intergenic 40.0%
!! TCCTCTGGTTTGTTTGTAAC+TGG + chr2_4:8560816-8560835 None:intergenic 40.0%
!! CCATAGCTATTTTGTGTCTG+AGG + chr2_4:8560855-8560874 None:intergenic 40.0%
CCTCAGACACAAAATAGCTA+TGG - chr2_4:8560852-8560871 Msa0313190:CDS 40.0%
AAGGAATGTGATCAGAGTCA+TGG + chr2_4:8560887-8560906 None:intergenic 40.0%
! TTGTGTCCCTCAACTCATTT+TGG + chr2_4:8561040-8561059 None:intergenic 40.0%
AGAAGGTTACAACTCCATGA+TGG - chr2_4:8561070-8561089 Msa0313190:CDS 40.0%
GCTCAGAATAACAGGTACAA+TGG - chr2_4:8561188-8561207 Msa0313190:CDS 40.0%
AGGTACAATGGTGAGAAACA+AGG - chr2_4:8561200-8561219 Msa0313190:CDS 40.0%
GGTACAATGGTGAGAAACAA+GGG - chr2_4:8561201-8561220 Msa0313190:CDS 40.0%
!! GACACAAGATTTTTGGAAGG+TGG - chr2_4:8561230-8561249 Msa0313190:CDS 40.0%
CAATGGTTACAAGAACCAAG+AGG - chr2_4:8561400-8561419 Msa0313190:CDS 40.0%
TTACAAGAACCAAGAGGAGT+TGG - chr2_4:8561406-8561425 Msa0313190:CDS 40.0%
! GTTCATTTTCCAACTCCTCT+TGG + chr2_4:8561418-8561437 None:intergenic 40.0%
GCTGAAGAATTCACTCTCTC+TGG + chr2_4:8560742-8560761 None:intergenic 45.0%
CCCAGTTACAAACAAACCAG+AGG - chr2_4:8560812-8560831 Msa0313190:CDS 45.0%
TGTGTCTGAGGAATGAAAGG+TGG + chr2_4:8560843-8560862 None:intergenic 45.0%
GCTGAAGAATTCACTCTCTC+TGG + chr2_4:8560742-8560761 None:intergenic 45.0%
CCCAGTTACAAACAAACCAG+AGG - chr2_4:8560812-8560831 Msa0313190:CDS 45.0%
TGTGTCTGAGGAATGAAAGG+TGG + chr2_4:8560843-8560862 None:intergenic 45.0%
ATGAAAGGTGGTTGCTCCTC+TGG + chr2_4:8560831-8560850 None:intergenic 50.0%
ATGAAAGGTGGTTGCTCCTC+TGG + chr2_4:8560831-8560850 None:intergenic 50.0%
!! AGAATTGGTGGTGGATGCCG+TGG + chr2_4:8560919-8560938 None:intergenic 55.0%
!! AGAATTGGTGGTGGATGCCG+TGG + chr2_4:8560919-8560938 None:intergenic 55.0%
!! ATTGGTGGTGGATGCCGTGG+CGG + chr2_4:8560916-8560935 None:intergenic 60.0%
!! ATTGGTGGTGGATGCCGTGG+CGG + chr2_4:8560916-8560935 None:intergenic 60.0%
CATTCCTTCCACCGCCGCCA+CGG - chr2_4:8560899-8560918 Msa0313190:CDS 65.0%
CATTCCTTCCACCGCCGCCA+CGG - chr2_4:8560899-8560918 Msa0313190:CDS 65.0%
GATGCCGTGGCGGCGGTGGA+AGG + chr2_4:8560906-8560925 None:intergenic 75.0%
!! GGTGGTGGATGCCGTGGCGG+CGG + chr2_4:8560913-8560932 None:intergenic 75.0%
GATGCCGTGGCGGCGGTGGA+AGG + chr2_4:8560906-8560925 None:intergenic 75.0%
!! GGTGGTGGATGCCGTGGCGG+CGG + chr2_4:8560913-8560932 None:intergenic 75.0%
! GGTGGATGCCGTGGCGGCGG+TGG + chr2_4:8560910-8560929 None:intergenic 80.0%
! GGTGGATGCCGTGGCGGCGG+TGG + chr2_4:8560910-8560929 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 8560669 8561463 8560669 ID=Msa0313190;Name=Msa0313190
chr2_4 mRNA 8560669 8561463 8560669 ID=Msa0313190-mRNA-1;Parent=Msa0313190;Name=Msa0313190-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|264
chr2_4 exon 8560669 8561463 8560669 ID=Msa0313190-mRNA-1:exon:3248;Parent=Msa0313190-mRNA-1
chr2_4 CDS 8560669 8561463 8560669 ID=Msa0313190-mRNA-1:cds;Parent=Msa0313190-mRNA-1
Gene Sequence

>Msa0313190

ATGGCCACATTCCCAACCTACATTTCTTTCATCCTCTTAACAACTCTTTTGTTGCCTCTACAAACTATAGCCAGAGAGAGTGAATTCTTCAGCAAAGTCACACATTTCAACAAAGAAACAAAAGTTCCCAACAAAGAAGAATCCCCAGTTACAAACAAACCAGAGGAGCAACCACCTTTCATTCCTCAGACACAAAATAGCTATGGCTTATATGGCCATGACTCTGATCACATTCCTTCCACCGCCGCCACGGCATCCACCACCAATTCTAACTTTGAAGACACAAACAATTACCCCAACAACAACAAATACTATAACAATGATGCATATAACACAAAGTATTACAACAAAGACACTTTTGGAAATAACCAAAATGAGTTGAGGGACACAAAGTACAATGAAGAAGGTTACAACTCCATGATGGAAAAACAAAACAACAACCAAGAGTACTACTTCAACAACAACAACAATGCTGCAAATGAAAGATCATTTTACAACAATAATTATAATAATTATAATGCTCAGAATAACAGGTACAATGGTGAGAAACAAGGGATGAGTGACACAAGATTTTTGGAAGGTGGAAAATATTTCTATGATATTAACTATGAGAAATACAATCCAACAATGTATGGTGATTCATCTAGAGGAGTGAACAACAATAATAACTGGTACAACAATAATAATAACTATGGTAACAACAATGAGTACCAGAATAATCATGGAAACTACAATGGTTACAAGAACCAAGAGGAGTTGGAAAATGAACAACATGAGTTTGATGTTTTACCATAA

Protein sequence

>Msa0313190

MATFPTYISFILLTTLLLPLQTIARESEFFSKVTHFNKETKVPNKEESPVTNKPEEQPPFIPQTQNSYGLYGHDSDHIPSTAATASTTNSNFEDTNNYPNNNKYYNNDAYNTKYYNKDTFGNNQNELRDTKYNEEGYNSMMEKQNNNQEYYFNNNNNAANERSFYNNNYNNYNAQNNRYNGEKQGMSDTRFLEGGKYFYDINYEKYNPTMYGDSSRGVNNNNNWYNNNNNYGNNNEYQNNHGNYNGYKNQEELENEQHEFDVLP*