AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0192570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192570 MtrunA17_Chr2g0282691 95.076 264 12 1 1 264 1 263 1.22e-110 319
Msa0192570 MtrunA17_Chr4g0046601 56.618 136 47 5 130 261 230 357 6.86e-22 93.2
Msa0192570 MtrunA17_Chr4g0046601 43.165 139 65 4 1 131 1 133 1.11e-18 84.3
Msa0192570 MtrunA17_Chr2g0324881 30.469 256 117 8 21 254 21 237 1.94e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192570 AT2G33850.1 31.429 210 101 7 21 202 22 216 5.85e-12 64.7

Find 51 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTGGTTTGTTTGTAAC+TGG 0.131657 2_1:+8371714 None:intergenic
TGTAGTTGCCATGGTTATTC+TGG 0.221088 2_1:+8371148 None:intergenic
TTGTGTCCCTCAACTCATTT+TGG 0.256754 2_1:+8371490 None:intergenic
CAAAATAGCTATGGCTTATA+TGG 0.260837 2_1:-8371666 Msa0192570:CDS
GTTGTAGTATTTGTTGTTGT+TGG 0.271949 2_1:+8371563 None:intergenic
TATTACAACAAAGACACTTT+TGG 0.320337 2_1:-8371519 Msa0192570:CDS
TTGTAGTATTTGTTGTTGTT+GGG 0.324049 2_1:+8371564 None:intergenic
AACTGGGGATTCTTCTTTGT+TGG 0.334551 2_1:+8371731 None:intergenic
ATGAAAGGTGGTTGCTCCTC+TGG 0.339057 2_1:+8371699 None:intergenic
ATTATAATGCTCAGAATAAC+AGG 0.351179 2_1:-8371347 Msa0192570:CDS
GGTGGATGCCGTGGCGGCGG+TGG 0.353742 2_1:+8371620 None:intergenic
ACTGGGGATTCTTCTTTGTT+GGG 0.370013 2_1:+8371732 None:intergenic
CCTCTGGTTTGTTTGTAACT+GGG 0.383487 2_1:+8371715 None:intergenic
TACAACAATAATAATAACTA+TGG 0.387734 2_1:-8371186 Msa0192570:CDS
GATGAATCACCATACATTGT+TGG 0.414049 2_1:+8371237 None:intergenic
GCTGAAGAATTCACTCTCTC+TGG 0.421980 2_1:+8371788 None:intergenic
GAGTGAACAACAATAATAAC+TGG 0.430385 2_1:-8371209 Msa0192570:CDS
TGTGTCTTCAAAGTTAGAAT+TGG 0.437689 2_1:+8371596 None:intergenic
AAATACAATCCAACAATGTA+TGG 0.452288 2_1:-8371246 Msa0192570:CDS
TAAGAGGATGAAAGAAATGT+AGG 0.456040 2_1:+8371842 None:intergenic
GATGCCGTGGCGGCGGTGGA+AGG 0.457928 2_1:+8371624 None:intergenic
GGTGGTGGATGCCGTGGCGG+CGG 0.460270 2_1:+8371617 None:intergenic
CTCTGGTTTGTTTGTAACTG+GGG 0.464914 2_1:+8371716 None:intergenic
AATTATTATAATTATTGTTG+TGG 0.469980 2_1:+8371367 None:intergenic
GTCTTCAAAGTTAGAATTGG+TGG 0.482976 2_1:+8371599 None:intergenic
TGTTGTTGAAGTAGTACTCT+TGG 0.484835 2_1:+8371418 None:intergenic
GCAACAAAAGAGTTGTTAAG+AGG 0.489505 2_1:+8371826 None:intergenic
AGGATGAAAGAAATGTAGGT+TGG 0.497294 2_1:+8371846 None:intergenic
GGATGAAAGAAATGTAGGTT+GGG 0.498004 2_1:+8371847 None:intergenic
TGTAACCATTGTAGTTGCCA+TGG 0.515915 2_1:+8371139 None:intergenic
AAATAACCAAAATGAGTTGA+GGG 0.518734 2_1:-8371496 Msa0192570:CDS
TGTAGTATTTGTTGTTGTTG+GGG 0.531822 2_1:+8371565 None:intergenic
GAAATAACCAAAATGAGTTG+AGG 0.542156 2_1:-8371497 Msa0192570:CDS
GACACAAAGTACAATGAAGA+AGG 0.550520 2_1:-8371474 Msa0192570:CDS
CCTCAGACACAAAATAGCTA+TGG 0.562030 2_1:-8371675 Msa0192570:CDS
CATTCCTTCCACCGCCGCCA+CGG 0.564976 2_1:-8371628 Msa0192570:CDS
AGAAGGTTACAACTCCATGA+TGG 0.580265 2_1:-8371457 Msa0192570:CDS
AGGTACAATGGTGAGAAACA+AGG 0.586625 2_1:-8371327 Msa0192570:CDS
TTCAAAGTTAGAATTGGTGG+TGG 0.587007 2_1:+8371602 None:intergenic
ATGTATGGTGATTCATCTAG+AGG 0.598645 2_1:-8371231 Msa0192570:CDS
TGTGTCTGAGGAATGAAAGG+TGG 0.603943 2_1:+8371687 None:intergenic
AATAACCATGGCAACTACAA+TGG 0.610902 2_1:-8371144 Msa0192570:CDS
GCTCAGAATAACAGGTACAA+TGG 0.619912 2_1:-8371339 Msa0192570:CDS
AAGGAATGTGATCAGAGTCA+TGG 0.626750 2_1:+8371643 None:intergenic
AGAATTGGTGGTGGATGCCG+TGG 0.633457 2_1:+8371611 None:intergenic
ATTGGTGGTGGATGCCGTGG+CGG 0.636866 2_1:+8371614 None:intergenic
GGTACAATGGTGAGAAACAA+GGG 0.647160 2_1:-8371326 Msa0192570:CDS
AGAAATGTAGGTTGGGAATG+TGG 0.649922 2_1:+8371854 None:intergenic
TCTCTGGCTATAGTTTGTAG+AGG 0.668021 2_1:+8371804 None:intergenic
AATGAGTACCAGAATAACCA+TGG 0.671041 2_1:-8371156 Msa0192570:CDS
CCCAGTTACAAACAAACCAG+AGG 0.679700 2_1:-8371715 Msa0192570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTATAATTATTGTTG+TGG + chr2_1:8371580-8371599 None:intergenic 10.0%
!! AATTATTATAATTATTGTTG+TGG + chr2_1:8371580-8371599 None:intergenic 10.0%
!! TACAACAATAATAATAACTA+TGG - chr2_1:8371758-8371777 Msa0192570:CDS 15.0%
!! TACAACAATAATAATAACTA+TGG - chr2_1:8371758-8371777 Msa0192570:CDS 15.0%
!!! TTGTAGTATTTGTTGTTGTT+GGG + chr2_1:8371383-8371402 None:intergenic 25.0%
!! TATTACAACAAAGACACTTT+TGG - chr2_1:8371425-8371444 Msa0192570:CDS 25.0%
!! AAATAACCAAAATGAGTTGA+GGG - chr2_1:8371448-8371467 Msa0192570:CDS 25.0%
! ATTATAATGCTCAGAATAAC+AGG - chr2_1:8371597-8371616 Msa0192570:CDS 25.0%
! AAATACAATCCAACAATGTA+TGG - chr2_1:8371698-8371717 Msa0192570:CDS 25.0%
!!! TTGTAGTATTTGTTGTTGTT+GGG + chr2_1:8371383-8371402 None:intergenic 25.0%
!! TATTACAACAAAGACACTTT+TGG - chr2_1:8371425-8371444 Msa0192570:CDS 25.0%
!! AAATAACCAAAATGAGTTGA+GGG - chr2_1:8371448-8371467 Msa0192570:CDS 25.0%
! ATTATAATGCTCAGAATAAC+AGG - chr2_1:8371597-8371616 Msa0192570:CDS 25.0%
! AAATACAATCCAACAATGTA+TGG - chr2_1:8371698-8371717 Msa0192570:CDS 25.0%
TAAGAGGATGAAAGAAATGT+AGG + chr2_1:8371105-8371124 None:intergenic 30.0%
CAAAATAGCTATGGCTTATA+TGG - chr2_1:8371278-8371297 Msa0192570:CDS 30.0%
TGTGTCTTCAAAGTTAGAAT+TGG + chr2_1:8371351-8371370 None:intergenic 30.0%
!! TGTAGTATTTGTTGTTGTTG+GGG + chr2_1:8371382-8371401 None:intergenic 30.0%
!! GTTGTAGTATTTGTTGTTGT+TGG + chr2_1:8371384-8371403 None:intergenic 30.0%
GAAATAACCAAAATGAGTTG+AGG - chr2_1:8371447-8371466 Msa0192570:CDS 30.0%
!!! GTTGTTTTGTTTTTCCATCA+TGG + chr2_1:8371504-8371523 None:intergenic 30.0%
GAGTGAACAACAATAATAAC+TGG - chr2_1:8371735-8371754 Msa0192570:CDS 30.0%
TAAGAGGATGAAAGAAATGT+AGG + chr2_1:8371105-8371124 None:intergenic 30.0%
CAAAATAGCTATGGCTTATA+TGG - chr2_1:8371278-8371297 Msa0192570:CDS 30.0%
TGTGTCTTCAAAGTTAGAAT+TGG + chr2_1:8371351-8371370 None:intergenic 30.0%
!! TGTAGTATTTGTTGTTGTTG+GGG + chr2_1:8371382-8371401 None:intergenic 30.0%
!! GTTGTAGTATTTGTTGTTGT+TGG + chr2_1:8371384-8371403 None:intergenic 30.0%
GAAATAACCAAAATGAGTTG+AGG - chr2_1:8371447-8371466 Msa0192570:CDS 30.0%
!!! GTTGTTTTGTTTTTCCATCA+TGG + chr2_1:8371504-8371523 None:intergenic 30.0%
GAGTGAACAACAATAATAAC+TGG - chr2_1:8371735-8371754 Msa0192570:CDS 30.0%
GGATGAAAGAAATGTAGGTT+GGG + chr2_1:8371100-8371119 None:intergenic 35.0%
AGGATGAAAGAAATGTAGGT+TGG + chr2_1:8371101-8371120 None:intergenic 35.0%
GCAACAAAAGAGTTGTTAAG+AGG + chr2_1:8371121-8371140 None:intergenic 35.0%
! TTTTGTGTCTGAGGAATGAA+AGG + chr2_1:8371263-8371282 None:intergenic 35.0%
TTCAAAGTTAGAATTGGTGG+TGG + chr2_1:8371345-8371364 None:intergenic 35.0%
GTCTTCAAAGTTAGAATTGG+TGG + chr2_1:8371348-8371367 None:intergenic 35.0%
GACACAAAGTACAATGAAGA+AGG - chr2_1:8371470-8371489 Msa0192570:CDS 35.0%
TGTTGTTGAAGTAGTACTCT+TGG + chr2_1:8371529-8371548 None:intergenic 35.0%
! GATGAGTGACACAAGATTTT+TGG - chr2_1:8371640-8371659 Msa0192570:CDS 35.0%
!! AGTGACACAAGATTTTTGGA+AGG - chr2_1:8371644-8371663 Msa0192570:CDS 35.0%
GATGAATCACCATACATTGT+TGG + chr2_1:8371710-8371729 None:intergenic 35.0%
ATGTATGGTGATTCATCTAG+AGG - chr2_1:8371713-8371732 Msa0192570:CDS 35.0%
AATGAGTACCAGAATAACCA+TGG - chr2_1:8371788-8371807 Msa0192570:CDS 35.0%
AATAACCATGGCAACTACAA+TGG - chr2_1:8371800-8371819 Msa0192570:CDS 35.0%
CAATGGTTACAAAAACCAAG+AGG - chr2_1:8371817-8371836 Msa0192570:CDS 35.0%
TTACAAAAACCAAGAGGAGT+TGG - chr2_1:8371823-8371842 Msa0192570:CDS 35.0%
GGATGAAAGAAATGTAGGTT+GGG + chr2_1:8371100-8371119 None:intergenic 35.0%
AGGATGAAAGAAATGTAGGT+TGG + chr2_1:8371101-8371120 None:intergenic 35.0%
GCAACAAAAGAGTTGTTAAG+AGG + chr2_1:8371121-8371140 None:intergenic 35.0%
! TTTTGTGTCTGAGGAATGAA+AGG + chr2_1:8371263-8371282 None:intergenic 35.0%
TTCAAAGTTAGAATTGGTGG+TGG + chr2_1:8371345-8371364 None:intergenic 35.0%
GTCTTCAAAGTTAGAATTGG+TGG + chr2_1:8371348-8371367 None:intergenic 35.0%
GACACAAAGTACAATGAAGA+AGG - chr2_1:8371470-8371489 Msa0192570:CDS 35.0%
TGTTGTTGAAGTAGTACTCT+TGG + chr2_1:8371529-8371548 None:intergenic 35.0%
! GATGAGTGACACAAGATTTT+TGG - chr2_1:8371640-8371659 Msa0192570:CDS 35.0%
!! AGTGACACAAGATTTTTGGA+AGG - chr2_1:8371644-8371663 Msa0192570:CDS 35.0%
GATGAATCACCATACATTGT+TGG + chr2_1:8371710-8371729 None:intergenic 35.0%
ATGTATGGTGATTCATCTAG+AGG - chr2_1:8371713-8371732 Msa0192570:CDS 35.0%
AATGAGTACCAGAATAACCA+TGG - chr2_1:8371788-8371807 Msa0192570:CDS 35.0%
AATAACCATGGCAACTACAA+TGG - chr2_1:8371800-8371819 Msa0192570:CDS 35.0%
CAATGGTTACAAAAACCAAG+AGG - chr2_1:8371817-8371836 Msa0192570:CDS 35.0%
TTACAAAAACCAAGAGGAGT+TGG - chr2_1:8371823-8371842 Msa0192570:CDS 35.0%
! AGAAATGTAGGTTGGGAATG+TGG + chr2_1:8371093-8371112 None:intergenic 40.0%
! TCTCTGGCTATAGTTTGTAG+AGG + chr2_1:8371143-8371162 None:intergenic 40.0%
ACTGGGGATTCTTCTTTGTT+GGG + chr2_1:8371215-8371234 None:intergenic 40.0%
AACTGGGGATTCTTCTTTGT+TGG + chr2_1:8371216-8371235 None:intergenic 40.0%
!! CTCTGGTTTGTTTGTAACTG+GGG + chr2_1:8371231-8371250 None:intergenic 40.0%
!! CCTCTGGTTTGTTTGTAACT+GGG + chr2_1:8371232-8371251 None:intergenic 40.0%
!! TCCTCTGGTTTGTTTGTAAC+TGG + chr2_1:8371233-8371252 None:intergenic 40.0%
!! CCATAGCTATTTTGTGTCTG+AGG + chr2_1:8371272-8371291 None:intergenic 40.0%
CCTCAGACACAAAATAGCTA+TGG - chr2_1:8371269-8371288 Msa0192570:CDS 40.0%
AAGGAATGTGATCAGAGTCA+TGG + chr2_1:8371304-8371323 None:intergenic 40.0%
! TTGTGTCCCTCAACTCATTT+TGG + chr2_1:8371457-8371476 None:intergenic 40.0%
AGAAGGTTACAACTCCATGA+TGG - chr2_1:8371487-8371506 Msa0192570:CDS 40.0%
GCTCAGAATAACAGGTACAA+TGG - chr2_1:8371605-8371624 Msa0192570:CDS 40.0%
AGGTACAATGGTGAGAAACA+AGG - chr2_1:8371617-8371636 Msa0192570:CDS 40.0%
GGTACAATGGTGAGAAACAA+GGG - chr2_1:8371618-8371637 Msa0192570:CDS 40.0%
!! GACACAAGATTTTTGGAAGG+TGG - chr2_1:8371647-8371666 Msa0192570:CDS 40.0%
TGTAGTTGCCATGGTTATTC+TGG + chr2_1:8371799-8371818 None:intergenic 40.0%
TGTAACCATTGTAGTTGCCA+TGG + chr2_1:8371808-8371827 None:intergenic 40.0%
! GTTCATTTTCCAACTCCTCT+TGG + chr2_1:8371835-8371854 None:intergenic 40.0%
! AGAAATGTAGGTTGGGAATG+TGG + chr2_1:8371093-8371112 None:intergenic 40.0%
! TCTCTGGCTATAGTTTGTAG+AGG + chr2_1:8371143-8371162 None:intergenic 40.0%
ACTGGGGATTCTTCTTTGTT+GGG + chr2_1:8371215-8371234 None:intergenic 40.0%
AACTGGGGATTCTTCTTTGT+TGG + chr2_1:8371216-8371235 None:intergenic 40.0%
!! CTCTGGTTTGTTTGTAACTG+GGG + chr2_1:8371231-8371250 None:intergenic 40.0%
!! CCTCTGGTTTGTTTGTAACT+GGG + chr2_1:8371232-8371251 None:intergenic 40.0%
!! TCCTCTGGTTTGTTTGTAAC+TGG + chr2_1:8371233-8371252 None:intergenic 40.0%
!! CCATAGCTATTTTGTGTCTG+AGG + chr2_1:8371272-8371291 None:intergenic 40.0%
CCTCAGACACAAAATAGCTA+TGG - chr2_1:8371269-8371288 Msa0192570:CDS 40.0%
AAGGAATGTGATCAGAGTCA+TGG + chr2_1:8371304-8371323 None:intergenic 40.0%
! TTGTGTCCCTCAACTCATTT+TGG + chr2_1:8371457-8371476 None:intergenic 40.0%
AGAAGGTTACAACTCCATGA+TGG - chr2_1:8371487-8371506 Msa0192570:CDS 40.0%
GCTCAGAATAACAGGTACAA+TGG - chr2_1:8371605-8371624 Msa0192570:CDS 40.0%
AGGTACAATGGTGAGAAACA+AGG - chr2_1:8371617-8371636 Msa0192570:CDS 40.0%
GGTACAATGGTGAGAAACAA+GGG - chr2_1:8371618-8371637 Msa0192570:CDS 40.0%
!! GACACAAGATTTTTGGAAGG+TGG - chr2_1:8371647-8371666 Msa0192570:CDS 40.0%
TGTAGTTGCCATGGTTATTC+TGG + chr2_1:8371799-8371818 None:intergenic 40.0%
TGTAACCATTGTAGTTGCCA+TGG + chr2_1:8371808-8371827 None:intergenic 40.0%
! GTTCATTTTCCAACTCCTCT+TGG + chr2_1:8371835-8371854 None:intergenic 40.0%
GCTGAAGAATTCACTCTCTC+TGG + chr2_1:8371159-8371178 None:intergenic 45.0%
CCCAGTTACAAACAAACCAG+AGG - chr2_1:8371229-8371248 Msa0192570:CDS 45.0%
TGTGTCTGAGGAATGAAAGG+TGG + chr2_1:8371260-8371279 None:intergenic 45.0%
GCTGAAGAATTCACTCTCTC+TGG + chr2_1:8371159-8371178 None:intergenic 45.0%
CCCAGTTACAAACAAACCAG+AGG - chr2_1:8371229-8371248 Msa0192570:CDS 45.0%
TGTGTCTGAGGAATGAAAGG+TGG + chr2_1:8371260-8371279 None:intergenic 45.0%
ATGAAAGGTGGTTGCTCCTC+TGG + chr2_1:8371248-8371267 None:intergenic 50.0%
ATGAAAGGTGGTTGCTCCTC+TGG + chr2_1:8371248-8371267 None:intergenic 50.0%
!! AGAATTGGTGGTGGATGCCG+TGG + chr2_1:8371336-8371355 None:intergenic 55.0%
!! AGAATTGGTGGTGGATGCCG+TGG + chr2_1:8371336-8371355 None:intergenic 55.0%
!! ATTGGTGGTGGATGCCGTGG+CGG + chr2_1:8371333-8371352 None:intergenic 60.0%
!! ATTGGTGGTGGATGCCGTGG+CGG + chr2_1:8371333-8371352 None:intergenic 60.0%
CATTCCTTCCACCGCCGCCA+CGG - chr2_1:8371316-8371335 Msa0192570:CDS 65.0%
CATTCCTTCCACCGCCGCCA+CGG - chr2_1:8371316-8371335 Msa0192570:CDS 65.0%
GATGCCGTGGCGGCGGTGGA+AGG + chr2_1:8371323-8371342 None:intergenic 75.0%
!! GGTGGTGGATGCCGTGGCGG+CGG + chr2_1:8371330-8371349 None:intergenic 75.0%
GATGCCGTGGCGGCGGTGGA+AGG + chr2_1:8371323-8371342 None:intergenic 75.0%
!! GGTGGTGGATGCCGTGGCGG+CGG + chr2_1:8371330-8371349 None:intergenic 75.0%
! GGTGGATGCCGTGGCGGCGG+TGG + chr2_1:8371327-8371346 None:intergenic 80.0%
! GGTGGATGCCGTGGCGGCGG+TGG + chr2_1:8371327-8371346 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 8371086 8371880 8371086 ID=Msa0192570;Name=Msa0192570
chr2_1 mRNA 8371086 8371880 8371086 ID=Msa0192570-mRNA-1;Parent=Msa0192570;Name=Msa0192570-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|264
chr2_1 exon 8371086 8371880 8371086 ID=Msa0192570-mRNA-1:exon:3839;Parent=Msa0192570-mRNA-1
chr2_1 CDS 8371086 8371880 8371086 ID=Msa0192570-mRNA-1:cds;Parent=Msa0192570-mRNA-1
Gene Sequence

>Msa0192570

ATGGCCACATTCCCAACCTACATTTCTTTCATCCTCTTAACAACTCTTTTGTTGCCTCTACAAACTATAGCCAGAGAGAGTGAATTCTTCAGCAAAGTCACACATTTCAACAAAGAAACAAAAGTTCCCAACAAAGAAGAATCCCCAGTTACAAACAAACCAGAGGAGCAACCACCTTTCATTCCTCAGACACAAAATAGCTATGGCTTATATGGCCATGACTCTGATCACATTCCTTCCACCGCCGCCACGGCATCCACCACCAATTCTAACTTTGAAGACACAAACAATTACCCCAACAACAACAAATACTACAACAATGATGCATATAACACAAAGTATTACAACAAAGACACTTTTGGAAATAACCAAAATGAGTTGAGGGACACAAAGTACAATGAAGAAGGTTACAACTCCATGATGGAAAAACAAAACAACAACCAAGAGTACTACTTCAACAACAACAACAATGCTGCAAATGAAAGATCATTCCACAACAATAATTATAATAATTATAATGCTCAGAATAACAGGTACAATGGTGAGAAACAAGGGATGAGTGACACAAGATTTTTGGAAGGTGGAAAATATTTCTATGATATTAACTATGAGAAATACAATCCAACAATGTATGGTGATTCATCTAGAGGAGTGAACAACAATAATAACTGGTACAACAATAATAATAACTATGGTAACAACAATGAGTACCAGAATAACCATGGCAACTACAATGGTTACAAAAACCAAGAGGAGTTGGAAAATGAACAACATGAGTTTGATGTTTTACCATAA

Protein sequence

>Msa0192570

MATFPTYISFILLTTLLLPLQTIARESEFFSKVTHFNKETKVPNKEESPVTNKPEEQPPFIPQTQNSYGLYGHDSDHIPSTAATASTTNSNFEDTNNYPNNNKYYNNDAYNTKYYNKDTFGNNQNELRDTKYNEEGYNSMMEKQNNNQEYYFNNNNNAANERSFHNNNYNNYNAQNNRYNGEKQGMSDTRFLEGGKYFYDINYEKYNPTMYGDSSRGVNNNNNWYNNNNNYGNNNEYQNNHGNYNGYKNQEELENEQHEFDVLP*