AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0272660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0272660 MtrunA17_Chr2g0282691 93.585 265 15 2 1 265 1 263 4.24e-109 315
Msa0272660 MtrunA17_Chr4g0046601 56.618 136 47 5 131 262 230 357 6.94e-22 93.2
Msa0272660 MtrunA17_Chr4g0046601 41.045 134 76 2 1 132 1 133 6.11e-18 82.4
Msa0272660 MtrunA17_Chr2g0324881 30.859 256 117 8 21 255 21 237 6.79e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0272660 AT2G33850.1 33.178 214 93 11 21 203 22 216 4.02e-11 62.4

Find 51 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTGGTTTGTTTGTAAC+TGG 0.131657 2_3:+4201281 None:intergenic
TGTAGTTGCCATGGTTATTC+TGG 0.221088 2_3:+4200712 None:intergenic
TTGTGTCCCTCAACTCATTT+TGG 0.256754 2_3:+4201054 None:intergenic
CAAAATAGCTATGGCTTATA+TGG 0.260837 2_3:-4201233 Msa0272660:CDS
TAGTACTTGTTATTGTTGTT+GGG 0.319644 2_3:+4201131 None:intergenic
GTAGTACTTGTTATTGTTGT+TGG 0.324905 2_3:+4201130 None:intergenic
TATTACAACAAAGACAGTTT+TGG 0.329701 2_3:-4201083 Msa0272660:CDS
AACTGGGGATTCTTCTTTGT+TGG 0.334551 2_3:+4201298 None:intergenic
ATGAAAGGTGGTTGCTCCTC+TGG 0.339057 2_3:+4201266 None:intergenic
ATTATAATGCTCAGAATAAC+AGG 0.351179 2_3:-4200911 Msa0272660:CDS
GGTGGATGCCGTGGCGGCGG+TGG 0.353742 2_3:+4201187 None:intergenic
ACTGGGGATTCTTCTTTGTT+GGG 0.370013 2_3:+4201299 None:intergenic
CCTCTGGTTTGTTTGTAACT+GGG 0.383487 2_3:+4201282 None:intergenic
TACAACAATAATAATAACTA+TGG 0.387734 2_3:-4200750 Msa0272660:CDS
GATGAATCACCATACATTGT+TGG 0.414049 2_3:+4200801 None:intergenic
TGTTTCTTCAAAGTTAGAAT+TGG 0.416528 2_3:+4201163 None:intergenic
GCTGAAGAATTCACTCTCTC+TGG 0.421980 2_3:+4201355 None:intergenic
GAGTGAACAACAATAATAAC+TGG 0.430385 2_3:-4200773 Msa0272660:CDS
TTCTTCAAAGTTAGAATTGG+TGG 0.439756 2_3:+4201166 None:intergenic
AAATACAATCCAACAATGTA+TGG 0.452288 2_3:-4200810 Msa0272660:CDS
TAAGAGGATGAAAGAAATGT+AGG 0.456040 2_3:+4201409 None:intergenic
GATGCCGTGGCGGCGGTGGA+AGG 0.457928 2_3:+4201191 None:intergenic
GGTGGTGGATGCCGTGGCGG+CGG 0.460270 2_3:+4201184 None:intergenic
CTCTGGTTTGTTTGTAACTG+GGG 0.464914 2_3:+4201283 None:intergenic
AATTATTATAATTATTGTTG+TGG 0.469980 2_3:+4200931 None:intergenic
TGTTGTTGAAGTAGTACTCT+TGG 0.484835 2_3:+4200982 None:intergenic
GCAACAAAAGAGTTGTTAAG+AGG 0.489505 2_3:+4201393 None:intergenic
AGGATGAAAGAAATGTAGGT+TGG 0.497294 2_3:+4201413 None:intergenic
GGATGAAAGAAATGTAGGTT+GGG 0.498004 2_3:+4201414 None:intergenic
TGTAACCATTGTAGTTGCCA+TGG 0.515915 2_3:+4200703 None:intergenic
AAATAACCAAAATGAGTTGA+GGG 0.518734 2_3:-4201060 Msa0272660:CDS
TTCAAAGTTAGAATTGGTGG+TGG 0.520386 2_3:+4201169 None:intergenic
AGTACTTGTTATTGTTGTTG+GGG 0.535885 2_3:+4201132 None:intergenic
GAAATAACCAAAATGAGTTG+AGG 0.542156 2_3:-4201061 Msa0272660:CDS
GACACAAAGTACAATGAAGA+AGG 0.550520 2_3:-4201038 Msa0272660:CDS
CCTCAGACACAAAATAGCTA+TGG 0.562030 2_3:-4201242 Msa0272660:CDS
CATTCCTTCCACCGCCGCCA+CGG 0.564976 2_3:-4201195 Msa0272660:CDS
AGAAGGTTACAACTCCATGA+TGG 0.580265 2_3:-4201021 Msa0272660:CDS
AGGTACAATGGTGAGAAACA+AGG 0.586625 2_3:-4200891 Msa0272660:CDS
ATGTATGGTGATTCATCTAG+AGG 0.598645 2_3:-4200795 Msa0272660:CDS
TGTGTCTGAGGAATGAAAGG+TGG 0.603943 2_3:+4201254 None:intergenic
AATAACCATGGCAACTACAA+TGG 0.610902 2_3:-4200708 Msa0272660:CDS
GCTCAGAATAACAGGTACAA+TGG 0.619912 2_3:-4200903 Msa0272660:CDS
AAGGAATGTGATCAGAGTCA+TGG 0.626750 2_3:+4201210 None:intergenic
AGAATTGGTGGTGGATGCCG+TGG 0.633457 2_3:+4201178 None:intergenic
ATTGGTGGTGGATGCCGTGG+CGG 0.636866 2_3:+4201181 None:intergenic
GGTACAATGGTGAGAAACAA+GGG 0.647160 2_3:-4200890 Msa0272660:CDS
AGAAATGTAGGTTGGGAATG+TGG 0.649922 2_3:+4201421 None:intergenic
TCTCTGGCTATAGTTTGTAG+AGG 0.668021 2_3:+4201371 None:intergenic
AATGAGTACCAGAATAACCA+TGG 0.671041 2_3:-4200720 Msa0272660:CDS
CCCAGTTACAAACAAACCAG+AGG 0.679700 2_3:-4201282 Msa0272660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTATAATTATTGTTG+TGG + chr2_3:4201147-4201166 None:intergenic 10.0%
!! AATTATTATAATTATTGTTG+TGG + chr2_3:4201147-4201166 None:intergenic 10.0%
!! TACAACAATAATAATAACTA+TGG - chr2_3:4201325-4201344 Msa0272660:CDS 15.0%
!! TACAACAATAATAATAACTA+TGG - chr2_3:4201325-4201344 Msa0272660:CDS 15.0%
! TGTTTCTTCAAAGTTAGAAT+TGG + chr2_3:4200915-4200934 None:intergenic 25.0%
!!! TAGTACTTGTTATTGTTGTT+GGG + chr2_3:4200947-4200966 None:intergenic 25.0%
!! TATTACAACAAAGACAGTTT+TGG - chr2_3:4200992-4201011 Msa0272660:CDS 25.0%
!! AAATAACCAAAATGAGTTGA+GGG - chr2_3:4201015-4201034 Msa0272660:CDS 25.0%
! ATTATAATGCTCAGAATAAC+AGG - chr2_3:4201164-4201183 Msa0272660:CDS 25.0%
! AAATACAATCCAACAATGTA+TGG - chr2_3:4201265-4201284 Msa0272660:CDS 25.0%
! TGTTTCTTCAAAGTTAGAAT+TGG + chr2_3:4200915-4200934 None:intergenic 25.0%
!!! TAGTACTTGTTATTGTTGTT+GGG + chr2_3:4200947-4200966 None:intergenic 25.0%
!! TATTACAACAAAGACAGTTT+TGG - chr2_3:4200992-4201011 Msa0272660:CDS 25.0%
!! AAATAACCAAAATGAGTTGA+GGG - chr2_3:4201015-4201034 Msa0272660:CDS 25.0%
! ATTATAATGCTCAGAATAAC+AGG - chr2_3:4201164-4201183 Msa0272660:CDS 25.0%
! AAATACAATCCAACAATGTA+TGG - chr2_3:4201265-4201284 Msa0272660:CDS 25.0%
TAAGAGGATGAAAGAAATGT+AGG + chr2_3:4200669-4200688 None:intergenic 30.0%
CAAAATAGCTATGGCTTATA+TGG - chr2_3:4200842-4200861 Msa0272660:CDS 30.0%
TTCTTCAAAGTTAGAATTGG+TGG + chr2_3:4200912-4200931 None:intergenic 30.0%
!! AGTACTTGTTATTGTTGTTG+GGG + chr2_3:4200946-4200965 None:intergenic 30.0%
!! GTAGTACTTGTTATTGTTGT+TGG + chr2_3:4200948-4200967 None:intergenic 30.0%
GAAATAACCAAAATGAGTTG+AGG - chr2_3:4201014-4201033 Msa0272660:CDS 30.0%
!!! GTTGTTTTGTTTTTCCATCA+TGG + chr2_3:4201071-4201090 None:intergenic 30.0%
GAGTGAACAACAATAATAAC+TGG - chr2_3:4201302-4201321 Msa0272660:CDS 30.0%
TAAGAGGATGAAAGAAATGT+AGG + chr2_3:4200669-4200688 None:intergenic 30.0%
CAAAATAGCTATGGCTTATA+TGG - chr2_3:4200842-4200861 Msa0272660:CDS 30.0%
TTCTTCAAAGTTAGAATTGG+TGG + chr2_3:4200912-4200931 None:intergenic 30.0%
!! AGTACTTGTTATTGTTGTTG+GGG + chr2_3:4200946-4200965 None:intergenic 30.0%
!! GTAGTACTTGTTATTGTTGT+TGG + chr2_3:4200948-4200967 None:intergenic 30.0%
GAAATAACCAAAATGAGTTG+AGG - chr2_3:4201014-4201033 Msa0272660:CDS 30.0%
!!! GTTGTTTTGTTTTTCCATCA+TGG + chr2_3:4201071-4201090 None:intergenic 30.0%
GAGTGAACAACAATAATAAC+TGG - chr2_3:4201302-4201321 Msa0272660:CDS 30.0%
GGATGAAAGAAATGTAGGTT+GGG + chr2_3:4200664-4200683 None:intergenic 35.0%
AGGATGAAAGAAATGTAGGT+TGG + chr2_3:4200665-4200684 None:intergenic 35.0%
GCAACAAAAGAGTTGTTAAG+AGG + chr2_3:4200685-4200704 None:intergenic 35.0%
! TTTTGTGTCTGAGGAATGAA+AGG + chr2_3:4200827-4200846 None:intergenic 35.0%
TTCAAAGTTAGAATTGGTGG+TGG + chr2_3:4200909-4200928 None:intergenic 35.0%
GACACAAAGTACAATGAAGA+AGG - chr2_3:4201037-4201056 Msa0272660:CDS 35.0%
TGTTGTTGAAGTAGTACTCT+TGG + chr2_3:4201096-4201115 None:intergenic 35.0%
! GATGAGTGACACAAGATTTT+TGG - chr2_3:4201207-4201226 Msa0272660:CDS 35.0%
!! AGTGACACAAGATTTTTGGA+AGG - chr2_3:4201211-4201230 Msa0272660:CDS 35.0%
GATGAATCACCATACATTGT+TGG + chr2_3:4201277-4201296 None:intergenic 35.0%
ATGTATGGTGATTCATCTAG+AGG - chr2_3:4201280-4201299 Msa0272660:CDS 35.0%
AATGAGTACCAGAATAACCA+TGG - chr2_3:4201355-4201374 Msa0272660:CDS 35.0%
AATAACCATGGCAACTACAA+TGG - chr2_3:4201367-4201386 Msa0272660:CDS 35.0%
CAATGGTTACAAAAACCAAG+AGG - chr2_3:4201384-4201403 Msa0272660:CDS 35.0%
TTACAAAAACCAAGAGGAGT+TGG - chr2_3:4201390-4201409 Msa0272660:CDS 35.0%
GGATGAAAGAAATGTAGGTT+GGG + chr2_3:4200664-4200683 None:intergenic 35.0%
AGGATGAAAGAAATGTAGGT+TGG + chr2_3:4200665-4200684 None:intergenic 35.0%
GCAACAAAAGAGTTGTTAAG+AGG + chr2_3:4200685-4200704 None:intergenic 35.0%
! TTTTGTGTCTGAGGAATGAA+AGG + chr2_3:4200827-4200846 None:intergenic 35.0%
TTCAAAGTTAGAATTGGTGG+TGG + chr2_3:4200909-4200928 None:intergenic 35.0%
GACACAAAGTACAATGAAGA+AGG - chr2_3:4201037-4201056 Msa0272660:CDS 35.0%
TGTTGTTGAAGTAGTACTCT+TGG + chr2_3:4201096-4201115 None:intergenic 35.0%
! GATGAGTGACACAAGATTTT+TGG - chr2_3:4201207-4201226 Msa0272660:CDS 35.0%
!! AGTGACACAAGATTTTTGGA+AGG - chr2_3:4201211-4201230 Msa0272660:CDS 35.0%
GATGAATCACCATACATTGT+TGG + chr2_3:4201277-4201296 None:intergenic 35.0%
ATGTATGGTGATTCATCTAG+AGG - chr2_3:4201280-4201299 Msa0272660:CDS 35.0%
AATGAGTACCAGAATAACCA+TGG - chr2_3:4201355-4201374 Msa0272660:CDS 35.0%
AATAACCATGGCAACTACAA+TGG - chr2_3:4201367-4201386 Msa0272660:CDS 35.0%
CAATGGTTACAAAAACCAAG+AGG - chr2_3:4201384-4201403 Msa0272660:CDS 35.0%
TTACAAAAACCAAGAGGAGT+TGG - chr2_3:4201390-4201409 Msa0272660:CDS 35.0%
! AGAAATGTAGGTTGGGAATG+TGG + chr2_3:4200657-4200676 None:intergenic 40.0%
! TCTCTGGCTATAGTTTGTAG+AGG + chr2_3:4200707-4200726 None:intergenic 40.0%
ACTGGGGATTCTTCTTTGTT+GGG + chr2_3:4200779-4200798 None:intergenic 40.0%
AACTGGGGATTCTTCTTTGT+TGG + chr2_3:4200780-4200799 None:intergenic 40.0%
!! CTCTGGTTTGTTTGTAACTG+GGG + chr2_3:4200795-4200814 None:intergenic 40.0%
!! CCTCTGGTTTGTTTGTAACT+GGG + chr2_3:4200796-4200815 None:intergenic 40.0%
!! TCCTCTGGTTTGTTTGTAAC+TGG + chr2_3:4200797-4200816 None:intergenic 40.0%
!! CCATAGCTATTTTGTGTCTG+AGG + chr2_3:4200836-4200855 None:intergenic 40.0%
CCTCAGACACAAAATAGCTA+TGG - chr2_3:4200833-4200852 Msa0272660:CDS 40.0%
AAGGAATGTGATCAGAGTCA+TGG + chr2_3:4200868-4200887 None:intergenic 40.0%
! TTGTGTCCCTCAACTCATTT+TGG + chr2_3:4201024-4201043 None:intergenic 40.0%
AGAAGGTTACAACTCCATGA+TGG - chr2_3:4201054-4201073 Msa0272660:CDS 40.0%
GCTCAGAATAACAGGTACAA+TGG - chr2_3:4201172-4201191 Msa0272660:CDS 40.0%
AGGTACAATGGTGAGAAACA+AGG - chr2_3:4201184-4201203 Msa0272660:CDS 40.0%
GGTACAATGGTGAGAAACAA+GGG - chr2_3:4201185-4201204 Msa0272660:CDS 40.0%
!! GACACAAGATTTTTGGAAGG+TGG - chr2_3:4201214-4201233 Msa0272660:CDS 40.0%
TGTAGTTGCCATGGTTATTC+TGG + chr2_3:4201366-4201385 None:intergenic 40.0%
TGTAACCATTGTAGTTGCCA+TGG + chr2_3:4201375-4201394 None:intergenic 40.0%
! GTTCATTTTCCAACTCCTCT+TGG + chr2_3:4201402-4201421 None:intergenic 40.0%
! AGAAATGTAGGTTGGGAATG+TGG + chr2_3:4200657-4200676 None:intergenic 40.0%
! TCTCTGGCTATAGTTTGTAG+AGG + chr2_3:4200707-4200726 None:intergenic 40.0%
ACTGGGGATTCTTCTTTGTT+GGG + chr2_3:4200779-4200798 None:intergenic 40.0%
AACTGGGGATTCTTCTTTGT+TGG + chr2_3:4200780-4200799 None:intergenic 40.0%
!! CTCTGGTTTGTTTGTAACTG+GGG + chr2_3:4200795-4200814 None:intergenic 40.0%
!! CCTCTGGTTTGTTTGTAACT+GGG + chr2_3:4200796-4200815 None:intergenic 40.0%
!! TCCTCTGGTTTGTTTGTAAC+TGG + chr2_3:4200797-4200816 None:intergenic 40.0%
!! CCATAGCTATTTTGTGTCTG+AGG + chr2_3:4200836-4200855 None:intergenic 40.0%
CCTCAGACACAAAATAGCTA+TGG - chr2_3:4200833-4200852 Msa0272660:CDS 40.0%
AAGGAATGTGATCAGAGTCA+TGG + chr2_3:4200868-4200887 None:intergenic 40.0%
! TTGTGTCCCTCAACTCATTT+TGG + chr2_3:4201024-4201043 None:intergenic 40.0%
AGAAGGTTACAACTCCATGA+TGG - chr2_3:4201054-4201073 Msa0272660:CDS 40.0%
GCTCAGAATAACAGGTACAA+TGG - chr2_3:4201172-4201191 Msa0272660:CDS 40.0%
AGGTACAATGGTGAGAAACA+AGG - chr2_3:4201184-4201203 Msa0272660:CDS 40.0%
GGTACAATGGTGAGAAACAA+GGG - chr2_3:4201185-4201204 Msa0272660:CDS 40.0%
!! GACACAAGATTTTTGGAAGG+TGG - chr2_3:4201214-4201233 Msa0272660:CDS 40.0%
TGTAGTTGCCATGGTTATTC+TGG + chr2_3:4201366-4201385 None:intergenic 40.0%
TGTAACCATTGTAGTTGCCA+TGG + chr2_3:4201375-4201394 None:intergenic 40.0%
! GTTCATTTTCCAACTCCTCT+TGG + chr2_3:4201402-4201421 None:intergenic 40.0%
GCTGAAGAATTCACTCTCTC+TGG + chr2_3:4200723-4200742 None:intergenic 45.0%
CCCAGTTACAAACAAACCAG+AGG - chr2_3:4200793-4200812 Msa0272660:CDS 45.0%
TGTGTCTGAGGAATGAAAGG+TGG + chr2_3:4200824-4200843 None:intergenic 45.0%
GCTGAAGAATTCACTCTCTC+TGG + chr2_3:4200723-4200742 None:intergenic 45.0%
CCCAGTTACAAACAAACCAG+AGG - chr2_3:4200793-4200812 Msa0272660:CDS 45.0%
TGTGTCTGAGGAATGAAAGG+TGG + chr2_3:4200824-4200843 None:intergenic 45.0%
ATGAAAGGTGGTTGCTCCTC+TGG + chr2_3:4200812-4200831 None:intergenic 50.0%
ATGAAAGGTGGTTGCTCCTC+TGG + chr2_3:4200812-4200831 None:intergenic 50.0%
!! AGAATTGGTGGTGGATGCCG+TGG + chr2_3:4200900-4200919 None:intergenic 55.0%
!! AGAATTGGTGGTGGATGCCG+TGG + chr2_3:4200900-4200919 None:intergenic 55.0%
!! ATTGGTGGTGGATGCCGTGG+CGG + chr2_3:4200897-4200916 None:intergenic 60.0%
!! ATTGGTGGTGGATGCCGTGG+CGG + chr2_3:4200897-4200916 None:intergenic 60.0%
CATTCCTTCCACCGCCGCCA+CGG - chr2_3:4200880-4200899 Msa0272660:CDS 65.0%
CATTCCTTCCACCGCCGCCA+CGG - chr2_3:4200880-4200899 Msa0272660:CDS 65.0%
GATGCCGTGGCGGCGGTGGA+AGG + chr2_3:4200887-4200906 None:intergenic 75.0%
!! GGTGGTGGATGCCGTGGCGG+CGG + chr2_3:4200894-4200913 None:intergenic 75.0%
GATGCCGTGGCGGCGGTGGA+AGG + chr2_3:4200887-4200906 None:intergenic 75.0%
!! GGTGGTGGATGCCGTGGCGG+CGG + chr2_3:4200894-4200913 None:intergenic 75.0%
! GGTGGATGCCGTGGCGGCGG+TGG + chr2_3:4200891-4200910 None:intergenic 80.0%
! GGTGGATGCCGTGGCGGCGG+TGG + chr2_3:4200891-4200910 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 4200650 4201447 4200650 ID=Msa0272660;Name=Msa0272660
chr2_3 mRNA 4200650 4201447 4200650 ID=Msa0272660-mRNA-1;Parent=Msa0272660;Name=Msa0272660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|265
chr2_3 exon 4200650 4201447 4200650 ID=Msa0272660-mRNA-1:exon:2104;Parent=Msa0272660-mRNA-1
chr2_3 CDS 4200650 4201447 4200650 ID=Msa0272660-mRNA-1:cds;Parent=Msa0272660-mRNA-1
Gene Sequence

>Msa0272660

ATGGCCACATTCCCAACCTACATTTCTTTCATCCTCTTAACAACTCTTTTGTTGCCTCTACAAACTATAGCCAGAGAGAGTGAATTCTTCAGCAAAGTCACACATTTCAACAAAGATACAAAAGTTCCCAACAAAGAAGAATCCCCAGTTACAAACAAACCAGAGGAGCAACCACCTTTCATTCCTCAGACACAAAATAGCTATGGCTTATATGGCCATGACTCTGATCACATTCCTTCCACCGCCGCCACGGCATCCACCACCAATTCTAACTTTGAAGAAACAAGCAATTACCCCAACAACAATAACAAGTACTACAACAATGATGCATATAACACAAAGTATTACAACAAAGACAGTTTTGGAAATAACCAAAATGAGTTGAGGGACACAAAGTACAATGAAGAAGGTTACAACTCCATGATGGAAAAACAAAACAACAACCAAGAGTACTACTTCAACAACAACAACAATGCTGCAAATGAAAGATCATTCCACAACAATAATTATAATAATTATAATGCTCAGAATAACAGGTACAATGGTGAGAAACAAGGGATGAGTGACACAAGATTTTTGGAAGGTGGAAAATATTTCTATGATATTAACTATGAGAAATACAATCCAACAATGTATGGTGATTCATCTAGAGGAGTGAACAACAATAATAACTGGTACAACAATAATAATAACTATGGTAACAACAATGAGTACCAGAATAACCATGGCAACTACAATGGTTACAAAAACCAAGAGGAGTTGGAAAATGAACAACATGAGTTTGATGTTTTACCATAA

Protein sequence

>Msa0272660

MATFPTYISFILLTTLLLPLQTIARESEFFSKVTHFNKDTKVPNKEESPVTNKPEEQPPFIPQTQNSYGLYGHDSDHIPSTAATASTTNSNFEETSNYPNNNNKYYNNDAYNTKYYNKDSFGNNQNELRDTKYNEEGYNSMMEKQNNNQEYYFNNNNNAANERSFHNNNYNNYNAQNNRYNGEKQGMSDTRFLEGGKYFYDINYEKYNPTMYGDSSRGVNNNNNWYNNNNNYGNNNEYQNNHGNYNGYKNQEELENEQHEFDVLP*