AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0317930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0317930 MtrunA17_Chr2g0291571 98.551 138 2 0 14 151 2 139 2.22e-101 286
Msa0317930 MtrunA17_Chr8g0380291 87.681 138 17 0 14 151 2 139 2.87e-92 263
Msa0317930 MtrunA17_Chr1g0182501 79.710 138 28 0 14 151 2 139 1.71e-83 241
Msa0317930 MtrunA17_Chr8g0386651 71.533 137 37 1 14 150 2 136 6.26e-75 219
Msa0317930 MtrunA17_Chr1g0203211 70.073 137 39 1 14 150 26 160 6.44e-71 210
Msa0317930 MtrunA17_Chr5g0397641 69.343 137 40 1 14 150 2 136 1.53e-70 208
Msa0317930 MtrunA17_Chr7g0261251 57.042 142 61 0 10 151 5 146 4.57e-64 192
Msa0317930 MtrunA17_Chr4g0037251 55.556 135 60 0 16 150 8 142 2.57e-56 172
Msa0317930 MtrunA17_Chr1g0187771 53.793 145 67 0 7 151 3 147 6.55e-52 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0317930 AT5G59880.1 80.435 138 27 0 14 151 2 139 1.92e-85 246
Msa0317930 AT3G46010.2 80.435 138 27 0 14 151 13 150 2.38e-85 247
Msa0317930 AT3G46010.1 80.435 138 27 0 14 151 2 139 3.66e-85 246
Msa0317930 AT5G59890.1 80.435 138 27 0 14 151 2 139 5.15e-83 240
Msa0317930 AT3G46000.1 78.986 138 27 1 14 151 2 137 9.53e-80 232
Msa0317930 AT3G46000.2 78.986 138 27 1 14 151 21 156 1.61e-79 232
Msa0317930 AT5G59890.3 79.545 132 27 0 20 151 1 132 3.01e-78 228
Msa0317930 AT5G59890.2 79.545 132 27 0 20 151 1 132 3.01e-78 228
Msa0317930 AT3G46000.3 78.030 132 27 1 20 151 1 130 5.27e-75 220
Msa0317930 AT4G00680.2 67.606 142 46 0 10 151 8 149 1.22e-74 219
Msa0317930 AT1G01750.2 67.361 144 47 0 8 151 1 144 4.01e-74 218
Msa0317930 AT4G00680.1 68.841 138 43 0 14 151 2 139 2.42e-73 216
Msa0317930 AT1G01750.1 69.565 138 42 0 14 151 2 139 4.14e-73 215
Msa0317930 AT4G25590.1 68.613 137 41 1 14 150 2 136 2.31e-71 211
Msa0317930 AT5G52360.1 69.343 137 40 1 14 150 2 136 3.58e-71 210
Msa0317930 AT5G59880.2 71.014 138 25 1 14 151 2 124 4.05e-71 210
Msa0317930 AT4G25590.2 67.176 131 41 1 20 150 1 129 1.18e-66 199
Msa0317930 AT2G31200.1 52.113 142 68 0 10 151 5 146 4.24e-57 175
Msa0317930 AT4G34970.1 54.015 137 63 0 15 151 5 141 1.31e-55 171
Msa0317930 AT2G16700.1 50.735 136 67 0 16 151 8 143 1.60e-52 163
Msa0317930 AT2G16700.4 50.758 132 65 0 20 151 1 132 1.46e-50 158
Msa0317930 AT2G16700.2 50.758 132 65 0 20 151 1 132 1.46e-50 158
Msa0317930 AT3G45990.1 56.522 138 49 3 20 151 1 133 5.57e-48 152
Msa0317930 AT2G16700.3 50.575 87 43 0 16 102 8 94 5.39e-30 105

Find 77 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACACTTTATGCCACAATTT+TGG 0.126450 2_4:+16102804 None:intergenic
TGACTGCAAGTTAAAGTTTA+TGG 0.155260 2_4:-16103398 Msa0317930:CDS
TCTGAATAGTATAGGCTTTC+TGG 0.160656 2_4:-16102919 Msa0317930:three_prime_UTR
GTTCATGCAAACTCTGTTTA+TGG 0.165538 2_4:-16102528 Msa0317930:three_prime_UTR
CTCCCTCTTGAACCTTTCTT+TGG 0.182021 2_4:+16103031 None:intergenic
CTTCTCACTCTTGCTGTATC+AGG 0.259069 2_4:+16103074 None:intergenic
TAGGCACAGTTGTCACTAAT+TGG 0.290153 2_4:-16102365 Msa0317930:three_prime_UTR
TTCATGCAAACTCTGTTTAT+GGG 0.320236 2_4:-16102527 Msa0317930:three_prime_UTR
GACTAGTGTCAATATTAAAA+AGG 0.322442 2_4:+16102631 None:intergenic
GTAGTGAGATCTTATTATAT+TGG 0.349128 2_4:-16102498 Msa0317930:three_prime_UTR
GAGCTTAGTTGTTGGGAGAA+AGG 0.358954 2_4:-16102428 Msa0317930:three_prime_UTR
CATAAAAGAGCTTAGTTGTT+GGG 0.361793 2_4:-16102435 Msa0317930:three_prime_UTR
GGCAACTGATCCTACTGAAA+TGG 0.363842 2_4:-16102982 Msa0317930:CDS
TGGTCAACTTGACTAGGTTA+TGG 0.369805 2_4:-16102838 Msa0317930:three_prime_UTR
GTTGACCAACCCACAAACTA+TGG 0.374300 2_4:+16102853 None:intergenic
AGGCATTATTTATACTTGAT+AGG 0.377844 2_4:+16102340 None:intergenic
ATTCAGAATCATTATTTAGT+TGG 0.389131 2_4:+16102935 None:intergenic
TGGAATTCAAATAGAGTTGC+AGG 0.390254 2_4:-16103003 Msa0317930:CDS
ATATGACTAACTAAGGCTTA+AGG 0.390867 2_4:+16102884 None:intergenic
TATGACTAACTAAGGCTTAA+GGG 0.394872 2_4:+16102885 None:intergenic
ATAGTCTCACGAATAGAATC+AGG 0.411616 2_4:-16102267 Msa0317930:three_prime_UTR
AAGTGTATTGCACCTTCAAC+AGG 0.412349 2_4:-16102788 Msa0317930:three_prime_UTR
TTAGTGACAACTGTGCCTAA+TGG 0.417440 2_4:+16102369 None:intergenic
TTATGGAGCTCAAGGCAAAA+AGG 0.429370 2_4:-16103381 Msa0317930:CDS
ACAAAACTCAAGCCCGCTAT+GGG 0.449583 2_4:+16102314 None:intergenic
AGTTACAAATTTACCCAGTA+AGG 0.449583 2_4:+16102689 None:intergenic
AACAAAACTCAAGCCCGCTA+TGG 0.467429 2_4:+16102313 None:intergenic
TGTGATGATCAGACAACAAA+TGG 0.468420 2_4:-16102660 Msa0317930:three_prime_UTR
GCAACTGATCCTACTGAAAT+GGG 0.470343 2_4:-16102981 Msa0317930:CDS
CTACAGGCAAATGCAGCATC+TGG 0.471777 2_4:-16103436 Msa0317930:CDS
ATGAAATGACTGAAGTCAAA+AGG 0.475626 2_4:+16102597 None:intergenic
GTTTCTAACTGCAATTCTAC+AGG 0.481385 2_4:-16103452 Msa0317930:CDS
ACTTTAACTTGCAGTCATCA+TGG 0.482180 2_4:+16103404 None:intergenic
TTGAGATATCCATAGTTTGT+GGG 0.483530 2_4:-16102862 Msa0317930:three_prime_UTR
ATAATGATTCTGAATAGTAT+AGG 0.484054 2_4:-16102927 Msa0317930:three_prime_UTR
TATCTCAATATGACTAACTA+AGG 0.495560 2_4:+16102877 None:intergenic
ATAATGCCTTTCCCCATAGC+GGG 0.501178 2_4:-16102326 Msa0317930:three_prime_UTR
GCATAAAAGAGCTTAGTTGT+TGG 0.501561 2_4:-16102436 Msa0317930:three_prime_UTR
GTTAAAGTTTATGGAGCTCA+AGG 0.502426 2_4:-16103389 Msa0317930:CDS
ATTGAGATATCCATAGTTTG+TGG 0.507811 2_4:-16102863 Msa0317930:three_prime_UTR
CGATCTTATACACTATGAAC+CGG 0.509573 2_4:+16103353 None:intergenic
TTTATGCAAGCACCAAAGAA+AGG 0.510363 2_4:-16103043 Msa0317930:CDS
TCGTGAGACTATATTACAAA+AGG 0.513033 2_4:+16102278 None:intergenic
GAGATCTTATTATATTGGTA+TGG 0.514317 2_4:-16102493 Msa0317930:three_prime_UTR
TAGTATAGGCTTTCTGGTGG+GGG 0.515785 2_4:-16102913 Msa0317930:three_prime_UTR
ATGCAATAAATAAAGTACTC+TGG 0.518889 2_4:+16102455 None:intergenic
GAACAAGAGAAGCCTGTTGA+AGG 0.524057 2_4:+16102776 None:intergenic
TTTCTGGTATGGGTATTCAA+AGG 0.528285 2_4:-16102565 Msa0317930:three_prime_UTR
GCATAACGGCACTCATCAGC+AGG 0.530155 2_4:+16103253 None:intergenic
GGCAAATGCAGCATCTGGTA+TGG 0.530905 2_4:-16103431 Msa0317930:CDS
CTCAAGCCCGCTATGGGGAA+AGG 0.536259 2_4:+16102320 None:intergenic
CAAGTCATTGTGGAAAAGCT+TGG 0.539988 2_4:-16103316 Msa0317930:CDS
ATAGTATAGGCTTTCTGGTG+GGG 0.545970 2_4:-16102914 Msa0317930:three_prime_UTR
GTGGGTTGGTCAACTTGACT+AGG 0.547849 2_4:-16102844 Msa0317930:three_prime_UTR
ATGACTAACTAAGGCTTAAG+GGG 0.552433 2_4:+16102886 None:intergenic
AGAAAGGTTCAAGAGGGAGC+TGG 0.559858 2_4:-16103027 Msa0317930:CDS
GAGTGATCAGTTAATCCATT+AGG 0.559965 2_4:-16102384 Msa0317930:three_prime_UTR
GAAACAGAAGCAAGTCATTG+TGG 0.565595 2_4:-16103326 Msa0317930:CDS
GATATCCATAGTTTGTGGGT+TGG 0.569687 2_4:-16102858 Msa0317930:three_prime_UTR
GAATAGTATAGGCTTTCTGG+TGG 0.570109 2_4:-16102916 Msa0317930:three_prime_UTR
GAATCATTATTTAGTTGGCA+CGG 0.570158 2_4:+16102940 None:intergenic
AATAGTATAGGCTTTCTGGT+GGG 0.571857 2_4:-16102915 Msa0317930:three_prime_UTR
GCACCAAAGAAAGGTTCAAG+AGG 0.572284 2_4:-16103034 Msa0317930:CDS
AATAATGCCTTTCCCCATAG+CGG 0.574104 2_4:-16102327 Msa0317930:three_prime_UTR
GTGATGATCAGACAACAAAT+GGG 0.575364 2_4:-16102659 Msa0317930:three_prime_UTR
TGCATGAACAAGACTACAGA+AGG 0.597126 2_4:+16102542 None:intergenic
AGGTTCAAGAGGGAGCTGGA+TGG 0.608161 2_4:-16103023 Msa0317930:CDS
AAGCTTGGTGAGCCAGCTGA+AGG 0.610538 2_4:-16103301 Msa0317930:CDS
CAAAATCATAGACAGCATAA+CGG 0.612434 2_4:+16103239 None:intergenic
ACATCAAGACCCATTTCAGT+AGG 0.621902 2_4:+16102972 None:intergenic
CACCAAAGAAAGGTTCAAGA+GGG 0.636143 2_4:-16103033 Msa0317930:CDS
AACGGCACTCATCAGCAGGA+AGG 0.636804 2_4:+16103257 None:intergenic
TAGTCTCACGAATAGAATCA+GGG 0.637285 2_4:-16102266 Msa0317930:three_prime_UTR
GTTCATAGTGTATAAGATCG+AGG 0.648241 2_4:-16103350 Msa0317930:CDS
CAAATCATAACAAAATACAC+AGG 0.664873 2_4:+16102754 None:intergenic
CAAAACTCAAGCCCGCTATG+GGG 0.686846 2_4:+16102315 None:intergenic
TGATGATCAGACAACAAATG+GGG 0.760183 2_4:-16102658 Msa0317930:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCATTTTTTTGTTTTTC+TGG - chr2_4:16103141-16103160 Msa0317930:intron 15.0%
!!! ATTTCATTTTTTTGTTTTTC+TGG - chr2_4:16103141-16103160 Msa0317930:intron 15.0%
!!! ATGAAAAAAATTCTGCTTTT+GGG + chr2_4:16102532-16102551 None:intergenic 20.0%
!!! AATGAAAAAAATTCTGCTTT+TGG + chr2_4:16102533-16102552 None:intergenic 20.0%
!!! AAAAGCAGAATTTTTTTCAT+TGG - chr2_4:16102532-16102551 Msa0317930:three_prime_UTR 20.0%
!!! AAAGCAGAATTTTTTTCATT+GGG - chr2_4:16102533-16102552 Msa0317930:three_prime_UTR 20.0%
!! ATTCAGAATCATTATTTAGT+TGG + chr2_4:16102790-16102809 None:intergenic 20.0%
!! ATAATGATTCTGAATAGTAT+AGG - chr2_4:16102795-16102814 Msa0317930:three_prime_UTR 20.0%
!!! ATTTTTTTGTTTTTCTGGTA+TGG - chr2_4:16103146-16103165 Msa0317930:intron 20.0%
!!! TTTTTTTGTTTTTCTGGTAT+GGG - chr2_4:16103147-16103166 Msa0317930:intron 20.0%
!!! ATGAAAAAAATTCTGCTTTT+GGG + chr2_4:16102532-16102551 None:intergenic 20.0%
!!! AATGAAAAAAATTCTGCTTT+TGG + chr2_4:16102533-16102552 None:intergenic 20.0%
!!! AAAAGCAGAATTTTTTTCAT+TGG - chr2_4:16102532-16102551 Msa0317930:three_prime_UTR 20.0%
!!! AAAGCAGAATTTTTTTCATT+GGG - chr2_4:16102533-16102552 Msa0317930:three_prime_UTR 20.0%
!! ATTCAGAATCATTATTTAGT+TGG + chr2_4:16102790-16102809 None:intergenic 20.0%
!! ATAATGATTCTGAATAGTAT+AGG - chr2_4:16102795-16102814 Msa0317930:three_prime_UTR 20.0%
!!! ATTTTTTTGTTTTTCTGGTA+TGG - chr2_4:16103146-16103165 Msa0317930:intron 20.0%
!!! TTTTTTTGTTTTTCTGGTAT+GGG - chr2_4:16103147-16103166 Msa0317930:intron 20.0%
!!! TGAAAAAAATTCTGCTTTTG+GGG + chr2_4:16102531-16102550 None:intergenic 25.0%
! TATCTCAATATGACTAACTA+AGG + chr2_4:16102848-16102867 None:intergenic 25.0%
!! TCTCAAATTTTCCAAAATTG+TGG - chr2_4:16102907-16102926 Msa0317930:three_prime_UTR 25.0%
! CAAATCATAACAAAATACAC+AGG + chr2_4:16102971-16102990 None:intergenic 25.0%
!!! CATTTTAGTATATCCTTACT+GGG - chr2_4:16103020-16103039 Msa0317930:CDS 25.0%
! GACTAGTGTCAATATTAAAA+AGG + chr2_4:16103094-16103113 None:intergenic 25.0%
! GTAGTGAGATCTTATTATAT+TGG - chr2_4:16103224-16103243 Msa0317930:CDS 25.0%
! GAGATCTTATTATATTGGTA+TGG - chr2_4:16103229-16103248 Msa0317930:CDS 25.0%
!! ATGCAATAAATAAAGTACTC+TGG + chr2_4:16103270-16103289 None:intergenic 25.0%
! AGGCATTATTTATACTTGAT+AGG + chr2_4:16103385-16103404 None:intergenic 25.0%
!!! TGAAAAAAATTCTGCTTTTG+GGG + chr2_4:16102531-16102550 None:intergenic 25.0%
! TATCTCAATATGACTAACTA+AGG + chr2_4:16102848-16102867 None:intergenic 25.0%
!! TCTCAAATTTTCCAAAATTG+TGG - chr2_4:16102907-16102926 Msa0317930:three_prime_UTR 25.0%
! CAAATCATAACAAAATACAC+AGG + chr2_4:16102971-16102990 None:intergenic 25.0%
!!! CATTTTAGTATATCCTTACT+GGG - chr2_4:16103020-16103039 Msa0317930:CDS 25.0%
! GACTAGTGTCAATATTAAAA+AGG + chr2_4:16103094-16103113 None:intergenic 25.0%
! GTAGTGAGATCTTATTATAT+TGG - chr2_4:16103224-16103243 Msa0317930:CDS 25.0%
! GAGATCTTATTATATTGGTA+TGG - chr2_4:16103229-16103248 Msa0317930:CDS 25.0%
!! ATGCAATAAATAAAGTACTC+TGG + chr2_4:16103270-16103289 None:intergenic 25.0%
! AGGCATTATTTATACTTGAT+AGG + chr2_4:16103385-16103404 None:intergenic 25.0%
TGACTGCAAGTTAAAGTTTA+TGG - chr2_4:16102324-16102343 Msa0317930:three_prime_UTR 30.0%
CAAAATCATAGACAGCATAA+CGG + chr2_4:16102486-16102505 None:intergenic 30.0%
!! AATGTTTGCTTTTCGATACA+TGG - chr2_4:16102560-16102579 Msa0317930:three_prime_UTR 30.0%
GAATCATTATTTAGTTGGCA+CGG + chr2_4:16102785-16102804 None:intergenic 30.0%
TATGACTAACTAAGGCTTAA+GGG + chr2_4:16102840-16102859 None:intergenic 30.0%
ATATGACTAACTAAGGCTTA+AGG + chr2_4:16102841-16102860 None:intergenic 30.0%
ATTGAGATATCCATAGTTTG+TGG - chr2_4:16102859-16102878 Msa0317930:three_prime_UTR 30.0%
! TTGAGATATCCATAGTTTGT+GGG - chr2_4:16102860-16102879 Msa0317930:three_prime_UTR 30.0%
! TACACTTTATGCCACAATTT+TGG + chr2_4:16102921-16102940 None:intergenic 30.0%
! ATACAATCTTTTCGTTTGAG+TGG - chr2_4:16102997-16103016 Msa0317930:CDS 30.0%
!! GCATTTTAGTATATCCTTAC+TGG - chr2_4:16103019-16103038 Msa0317930:CDS 30.0%
AGTTACAAATTTACCCAGTA+AGG + chr2_4:16103036-16103055 None:intergenic 30.0%
ATGAAATGACTGAAGTCAAA+AGG + chr2_4:16103128-16103147 None:intergenic 30.0%
TTCATGCAAACTCTGTTTAT+GGG - chr2_4:16103195-16103214 Msa0317930:CDS 30.0%
CATAAAAGAGCTTAGTTGTT+GGG - chr2_4:16103287-16103306 Msa0317930:CDS 30.0%
TCGTGAGACTATATTACAAA+AGG + chr2_4:16103447-16103466 None:intergenic 30.0%
TGACTGCAAGTTAAAGTTTA+TGG - chr2_4:16102324-16102343 Msa0317930:three_prime_UTR 30.0%
CAAAATCATAGACAGCATAA+CGG + chr2_4:16102486-16102505 None:intergenic 30.0%
!! AATGTTTGCTTTTCGATACA+TGG - chr2_4:16102560-16102579 Msa0317930:three_prime_UTR 30.0%
GAATCATTATTTAGTTGGCA+CGG + chr2_4:16102785-16102804 None:intergenic 30.0%
TATGACTAACTAAGGCTTAA+GGG + chr2_4:16102840-16102859 None:intergenic 30.0%
ATATGACTAACTAAGGCTTA+AGG + chr2_4:16102841-16102860 None:intergenic 30.0%
ATTGAGATATCCATAGTTTG+TGG - chr2_4:16102859-16102878 Msa0317930:three_prime_UTR 30.0%
! TTGAGATATCCATAGTTTGT+GGG - chr2_4:16102860-16102879 Msa0317930:three_prime_UTR 30.0%
! TACACTTTATGCCACAATTT+TGG + chr2_4:16102921-16102940 None:intergenic 30.0%
! ATACAATCTTTTCGTTTGAG+TGG - chr2_4:16102997-16103016 Msa0317930:CDS 30.0%
!! GCATTTTAGTATATCCTTAC+TGG - chr2_4:16103019-16103038 Msa0317930:CDS 30.0%
AGTTACAAATTTACCCAGTA+AGG + chr2_4:16103036-16103055 None:intergenic 30.0%
ATGAAATGACTGAAGTCAAA+AGG + chr2_4:16103128-16103147 None:intergenic 30.0%
TTCATGCAAACTCTGTTTAT+GGG - chr2_4:16103195-16103214 Msa0317930:CDS 30.0%
CATAAAAGAGCTTAGTTGTT+GGG - chr2_4:16103287-16103306 Msa0317930:CDS 30.0%
TCGTGAGACTATATTACAAA+AGG + chr2_4:16103447-16103466 None:intergenic 30.0%
GTTTCTAACTGCAATTCTAC+AGG - chr2_4:16102270-16102289 Msa0317930:three_prime_UTR 35.0%
!! ACTTTAACTTGCAGTCATCA+TGG + chr2_4:16102321-16102340 None:intergenic 35.0%
GTTAAAGTTTATGGAGCTCA+AGG - chr2_4:16102333-16102352 Msa0317930:three_prime_UTR 35.0%
CGATCTTATACACTATGAAC+CGG + chr2_4:16102372-16102391 None:intergenic 35.0%
GTTCATAGTGTATAAGATCG+AGG - chr2_4:16102372-16102391 Msa0317930:three_prime_UTR 35.0%
! AAATTTTCATAGCCTTCAGC+TGG + chr2_4:16102436-16102455 None:intergenic 35.0%
!!! GCAGAATTTTTTTCATTGGG+TGG - chr2_4:16102536-16102555 Msa0317930:three_prime_UTR 35.0%
! TACATGGCTATAGTTTTCCT+TGG - chr2_4:16102576-16102595 Msa0317930:three_prime_UTR 35.0%
AGATAGGAAGTAAACAACCA+AGG + chr2_4:16102596-16102615 None:intergenic 35.0%
CCAACAGAATTAATGCAGAT+AGG + chr2_4:16102612-16102631 None:intergenic 35.0%
CCTATCTGCATTAATTCTGT+TGG - chr2_4:16102609-16102628 Msa0317930:three_prime_UTR 35.0%
TTTATGCAAGCACCAAAGAA+AGG - chr2_4:16102679-16102698 Msa0317930:three_prime_UTR 35.0%
TGGAATTCAAATAGAGTTGC+AGG - chr2_4:16102719-16102738 Msa0317930:three_prime_UTR 35.0%
TCTGAATAGTATAGGCTTTC+TGG - chr2_4:16102803-16102822 Msa0317930:three_prime_UTR 35.0%
! AATAGTATAGGCTTTCTGGT+GGG - chr2_4:16102807-16102826 Msa0317930:three_prime_UTR 35.0%
ATGACTAACTAAGGCTTAAG+GGG + chr2_4:16102839-16102858 None:intergenic 35.0%
TGTGATGATCAGACAACAAA+TGG - chr2_4:16103062-16103081 Msa0317930:CDS 35.0%
GTGATGATCAGACAACAAAT+GGG - chr2_4:16103063-16103082 Msa0317930:CDS 35.0%
TGATGATCAGACAACAAATG+GGG - chr2_4:16103064-16103083 Msa0317930:CDS 35.0%
! TTTCTGGTATGGGTATTCAA+AGG - chr2_4:16103157-16103176 Msa0317930:intron 35.0%
GTTCATGCAAACTCTGTTTA+TGG - chr2_4:16103194-16103213 Msa0317930:CDS 35.0%
GCATAAAAGAGCTTAGTTGT+TGG - chr2_4:16103286-16103305 Msa0317930:CDS 35.0%
GAGTGATCAGTTAATCCATT+AGG - chr2_4:16103338-16103357 Msa0317930:CDS 35.0%
ATAGTCTCACGAATAGAATC+AGG - chr2_4:16103455-16103474 Msa0317930:CDS 35.0%
TAGTCTCACGAATAGAATCA+GGG - chr2_4:16103456-16103475 Msa0317930:CDS 35.0%
GTTTCTAACTGCAATTCTAC+AGG - chr2_4:16102270-16102289 Msa0317930:three_prime_UTR 35.0%
!! ACTTTAACTTGCAGTCATCA+TGG + chr2_4:16102321-16102340 None:intergenic 35.0%
GTTAAAGTTTATGGAGCTCA+AGG - chr2_4:16102333-16102352 Msa0317930:three_prime_UTR 35.0%
CGATCTTATACACTATGAAC+CGG + chr2_4:16102372-16102391 None:intergenic 35.0%
GTTCATAGTGTATAAGATCG+AGG - chr2_4:16102372-16102391 Msa0317930:three_prime_UTR 35.0%
! AAATTTTCATAGCCTTCAGC+TGG + chr2_4:16102436-16102455 None:intergenic 35.0%
!!! GCAGAATTTTTTTCATTGGG+TGG - chr2_4:16102536-16102555 Msa0317930:three_prime_UTR 35.0%
! TACATGGCTATAGTTTTCCT+TGG - chr2_4:16102576-16102595 Msa0317930:three_prime_UTR 35.0%
AGATAGGAAGTAAACAACCA+AGG + chr2_4:16102596-16102615 None:intergenic 35.0%
CCAACAGAATTAATGCAGAT+AGG + chr2_4:16102612-16102631 None:intergenic 35.0%
CCTATCTGCATTAATTCTGT+TGG - chr2_4:16102609-16102628 Msa0317930:three_prime_UTR 35.0%
TTTATGCAAGCACCAAAGAA+AGG - chr2_4:16102679-16102698 Msa0317930:three_prime_UTR 35.0%
TGGAATTCAAATAGAGTTGC+AGG - chr2_4:16102719-16102738 Msa0317930:three_prime_UTR 35.0%
TCTGAATAGTATAGGCTTTC+TGG - chr2_4:16102803-16102822 Msa0317930:three_prime_UTR 35.0%
! AATAGTATAGGCTTTCTGGT+GGG - chr2_4:16102807-16102826 Msa0317930:three_prime_UTR 35.0%
ATGACTAACTAAGGCTTAAG+GGG + chr2_4:16102839-16102858 None:intergenic 35.0%
TGTGATGATCAGACAACAAA+TGG - chr2_4:16103062-16103081 Msa0317930:CDS 35.0%
GTGATGATCAGACAACAAAT+GGG - chr2_4:16103063-16103082 Msa0317930:CDS 35.0%
TGATGATCAGACAACAAATG+GGG - chr2_4:16103064-16103083 Msa0317930:CDS 35.0%
! TTTCTGGTATGGGTATTCAA+AGG - chr2_4:16103157-16103176 Msa0317930:intron 35.0%
GTTCATGCAAACTCTGTTTA+TGG - chr2_4:16103194-16103213 Msa0317930:CDS 35.0%
GCATAAAAGAGCTTAGTTGT+TGG - chr2_4:16103286-16103305 Msa0317930:CDS 35.0%
GAGTGATCAGTTAATCCATT+AGG - chr2_4:16103338-16103357 Msa0317930:CDS 35.0%
ATAGTCTCACGAATAGAATC+AGG - chr2_4:16103455-16103474 Msa0317930:CDS 35.0%
TAGTCTCACGAATAGAATCA+GGG - chr2_4:16103456-16103475 Msa0317930:CDS 35.0%
TTATGGAGCTCAAGGCAAAA+AGG - chr2_4:16102341-16102360 Msa0317930:three_prime_UTR 40.0%
GAAACAGAAGCAAGTCATTG+TGG - chr2_4:16102396-16102415 Msa0317930:three_prime_UTR 40.0%
CAAGTCATTGTGGAAAAGCT+TGG - chr2_4:16102406-16102425 Msa0317930:three_prime_UTR 40.0%
!! ATTCTGTTGGCTTTGTGTGT+AGG - chr2_4:16102622-16102641 Msa0317930:three_prime_UTR 40.0%
CACCAAAGAAAGGTTCAAGA+GGG - chr2_4:16102689-16102708 Msa0317930:three_prime_UTR 40.0%
GCAACTGATCCTACTGAAAT+GGG - chr2_4:16102741-16102760 Msa0317930:three_prime_UTR 40.0%
ACATCAAGACCCATTTCAGT+AGG + chr2_4:16102753-16102772 None:intergenic 40.0%
GAATAGTATAGGCTTTCTGG+TGG - chr2_4:16102806-16102825 Msa0317930:three_prime_UTR 40.0%
! ATAGTATAGGCTTTCTGGTG+GGG - chr2_4:16102808-16102827 Msa0317930:three_prime_UTR 40.0%
! GATATCCATAGTTTGTGGGT+TGG - chr2_4:16102864-16102883 Msa0317930:three_prime_UTR 40.0%
TGGTCAACTTGACTAGGTTA+TGG - chr2_4:16102884-16102903 Msa0317930:three_prime_UTR 40.0%
AAGTGTATTGCACCTTCAAC+AGG - chr2_4:16102934-16102953 Msa0317930:exon 40.0%
TGCATGAACAAGACTACAGA+AGG + chr2_4:16103183-16103202 None:intergenic 40.0%
TTAGTGACAACTGTGCCTAA+TGG + chr2_4:16103356-16103375 None:intergenic 40.0%
TAGGCACAGTTGTCACTAAT+TGG - chr2_4:16103357-16103376 Msa0317930:CDS 40.0%
AATAATGCCTTTCCCCATAG+CGG - chr2_4:16103395-16103414 Msa0317930:CDS 40.0%
TTATGGAGCTCAAGGCAAAA+AGG - chr2_4:16102341-16102360 Msa0317930:three_prime_UTR 40.0%
GAAACAGAAGCAAGTCATTG+TGG - chr2_4:16102396-16102415 Msa0317930:three_prime_UTR 40.0%
CAAGTCATTGTGGAAAAGCT+TGG - chr2_4:16102406-16102425 Msa0317930:three_prime_UTR 40.0%
!! ATTCTGTTGGCTTTGTGTGT+AGG - chr2_4:16102622-16102641 Msa0317930:three_prime_UTR 40.0%
CACCAAAGAAAGGTTCAAGA+GGG - chr2_4:16102689-16102708 Msa0317930:three_prime_UTR 40.0%
GCAACTGATCCTACTGAAAT+GGG - chr2_4:16102741-16102760 Msa0317930:three_prime_UTR 40.0%
ACATCAAGACCCATTTCAGT+AGG + chr2_4:16102753-16102772 None:intergenic 40.0%
GAATAGTATAGGCTTTCTGG+TGG - chr2_4:16102806-16102825 Msa0317930:three_prime_UTR 40.0%
! ATAGTATAGGCTTTCTGGTG+GGG - chr2_4:16102808-16102827 Msa0317930:three_prime_UTR 40.0%
! GATATCCATAGTTTGTGGGT+TGG - chr2_4:16102864-16102883 Msa0317930:three_prime_UTR 40.0%
TGGTCAACTTGACTAGGTTA+TGG - chr2_4:16102884-16102903 Msa0317930:three_prime_UTR 40.0%
AAGTGTATTGCACCTTCAAC+AGG - chr2_4:16102934-16102953 Msa0317930:exon 40.0%
TGCATGAACAAGACTACAGA+AGG + chr2_4:16103183-16103202 None:intergenic 40.0%
TTAGTGACAACTGTGCCTAA+TGG + chr2_4:16103356-16103375 None:intergenic 40.0%
TAGGCACAGTTGTCACTAAT+TGG - chr2_4:16103357-16103376 Msa0317930:CDS 40.0%
AATAATGCCTTTCCCCATAG+CGG - chr2_4:16103395-16103414 Msa0317930:CDS 40.0%
TCAAGGCAAAAAGGACACAC+CGG - chr2_4:16102350-16102369 Msa0317930:three_prime_UTR 45.0%
! CTTCTCACTCTTGCTGTATC+AGG + chr2_4:16102651-16102670 None:intergenic 45.0%
GCACCAAAGAAAGGTTCAAG+AGG - chr2_4:16102688-16102707 Msa0317930:three_prime_UTR 45.0%
CTCCCTCTTGAACCTTTCTT+TGG + chr2_4:16102694-16102713 None:intergenic 45.0%
GGCAACTGATCCTACTGAAA+TGG - chr2_4:16102740-16102759 Msa0317930:three_prime_UTR 45.0%
! TAGTATAGGCTTTCTGGTGG+GGG - chr2_4:16102809-16102828 Msa0317930:three_prime_UTR 45.0%
GTTGACCAACCCACAAACTA+TGG + chr2_4:16102872-16102891 None:intergenic 45.0%
GAACAAGAGAAGCCTGTTGA+AGG + chr2_4:16102949-16102968 None:intergenic 45.0%
GAGCTTAGTTGTTGGGAGAA+AGG - chr2_4:16103294-16103313 Msa0317930:CDS 45.0%
ATAATGCCTTTCCCCATAGC+GGG - chr2_4:16103396-16103415 Msa0317930:CDS 45.0%
ACAAAACTCAAGCCCGCTAT+GGG + chr2_4:16103411-16103430 None:intergenic 45.0%
AACAAAACTCAAGCCCGCTA+TGG + chr2_4:16103412-16103431 None:intergenic 45.0%
TCAAGGCAAAAAGGACACAC+CGG - chr2_4:16102350-16102369 Msa0317930:three_prime_UTR 45.0%
! CTTCTCACTCTTGCTGTATC+AGG + chr2_4:16102651-16102670 None:intergenic 45.0%
GCACCAAAGAAAGGTTCAAG+AGG - chr2_4:16102688-16102707 Msa0317930:three_prime_UTR 45.0%
CTCCCTCTTGAACCTTTCTT+TGG + chr2_4:16102694-16102713 None:intergenic 45.0%
GGCAACTGATCCTACTGAAA+TGG - chr2_4:16102740-16102759 Msa0317930:three_prime_UTR 45.0%
! TAGTATAGGCTTTCTGGTGG+GGG - chr2_4:16102809-16102828 Msa0317930:three_prime_UTR 45.0%
GTTGACCAACCCACAAACTA+TGG + chr2_4:16102872-16102891 None:intergenic 45.0%
GAACAAGAGAAGCCTGTTGA+AGG + chr2_4:16102949-16102968 None:intergenic 45.0%
GAGCTTAGTTGTTGGGAGAA+AGG - chr2_4:16103294-16103313 Msa0317930:CDS 45.0%
ATAATGCCTTTCCCCATAGC+GGG - chr2_4:16103396-16103415 Msa0317930:CDS 45.0%
ACAAAACTCAAGCCCGCTAT+GGG + chr2_4:16103411-16103430 None:intergenic 45.0%
AACAAAACTCAAGCCCGCTA+TGG + chr2_4:16103412-16103431 None:intergenic 45.0%
CTACAGGCAAATGCAGCATC+TGG - chr2_4:16102286-16102305 Msa0317930:three_prime_UTR 50.0%
GGCAAATGCAGCATCTGGTA+TGG - chr2_4:16102291-16102310 Msa0317930:three_prime_UTR 50.0%
AGAAAGGTTCAAGAGGGAGC+TGG - chr2_4:16102695-16102714 Msa0317930:three_prime_UTR 50.0%
!! GTGGGTTGGTCAACTTGACT+AGG - chr2_4:16102878-16102897 Msa0317930:three_prime_UTR 50.0%
CAAAACTCAAGCCCGCTATG+GGG + chr2_4:16103410-16103429 None:intergenic 50.0%
CTACAGGCAAATGCAGCATC+TGG - chr2_4:16102286-16102305 Msa0317930:three_prime_UTR 50.0%
GGCAAATGCAGCATCTGGTA+TGG - chr2_4:16102291-16102310 Msa0317930:three_prime_UTR 50.0%
AGAAAGGTTCAAGAGGGAGC+TGG - chr2_4:16102695-16102714 Msa0317930:three_prime_UTR 50.0%
!! GTGGGTTGGTCAACTTGACT+AGG - chr2_4:16102878-16102897 Msa0317930:three_prime_UTR 50.0%
CAAAACTCAAGCCCGCTATG+GGG + chr2_4:16103410-16103429 None:intergenic 50.0%
! AAGCTTGGTGAGCCAGCTGA+AGG - chr2_4:16102421-16102440 Msa0317930:three_prime_UTR 55.0%
AACGGCACTCATCAGCAGGA+AGG + chr2_4:16102468-16102487 None:intergenic 55.0%
GCATAACGGCACTCATCAGC+AGG + chr2_4:16102472-16102491 None:intergenic 55.0%
AGGTTCAAGAGGGAGCTGGA+TGG - chr2_4:16102699-16102718 Msa0317930:three_prime_UTR 55.0%
! AAGCTTGGTGAGCCAGCTGA+AGG - chr2_4:16102421-16102440 Msa0317930:three_prime_UTR 55.0%
AACGGCACTCATCAGCAGGA+AGG + chr2_4:16102468-16102487 None:intergenic 55.0%
GCATAACGGCACTCATCAGC+AGG + chr2_4:16102472-16102491 None:intergenic 55.0%
AGGTTCAAGAGGGAGCTGGA+TGG - chr2_4:16102699-16102718 Msa0317930:three_prime_UTR 55.0%
CTCAAGCCCGCTATGGGGAA+AGG + chr2_4:16103405-16103424 None:intergenic 60.0%
CTCAAGCCCGCTATGGGGAA+AGG + chr2_4:16103405-16103424 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 16102253 16103491 16102253 ID=Msa0317930;Name=Msa0317930
chr2_4 mRNA 16102253 16103491 16102253 ID=Msa0317930-mRNA-1;Parent=Msa0317930;Name=Msa0317930-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|1|0.5|1|1|1|2|697|151
chr2_4 exon 16102253 16103100 16102253 ID=Msa0317930-mRNA-1:exon:6039;Parent=Msa0317930-mRNA-1
chr2_4 exon 16103187 16103491 16103187 ID=Msa0317930-mRNA-1:exon:6038;Parent=Msa0317930-mRNA-1
chr2_4 CDS 16103187 16103491 16103187 ID=Msa0317930-mRNA-1:cds;Parent=Msa0317930-mRNA-1
chr2_4 CDS 16102950 16103100 16102950 ID=Msa0317930-mRNA-1:cds;Parent=Msa0317930-mRNA-1
chr2_4 three_prime_UTR 16102253 16102949 16102253 ID=Msa0317930-mRNA-1:three_prime_utr;Parent=Msa0317930-mRNA-1
Gene Sequence

>Msa0317930

ATGATTGATTATGATGTGTTTCTAACTGCAATTCTACAGGCAAATGCAGCATCTGGTATGGCAGTCCATGATGACTGCAAGTTAAAGTTTATGGAGCTCAAGGCAAAAAGGACACACCGGTTCATAGTGTATAAGATCGAGGAGAAACAGAAGCAAGTCATTGTGGAAAAGCTTGGTGAGCCAGCTGAAGGCTATGAAAATTTCACTGCATGCCTTCCTGCTGATGAGTGCCGTTATGCTGTCTATGATTTTGAGTATCTGACAGAAGAGAATGTCCCCAAAAGCAGAATTTTTTTCATTGGGTGGTCGCCTGATACAGCAAGAGTGAGAAGCAAGATGATTTATGCAAGCACCAAAGAAAGGTTCAAGAGGGAGCTGGATGGAATTCAAATAGAGTTGCAGGCAACTGATCCTACTGAAATGGGTCTTGATGTGTTCAAAAGCCGTGCCAACTAA
Protein sequence

>Msa0317930

MIDYDVFLTAILQANAASGMAVHDDCKLKFMELKAKRTHRFIVYKIEEKQKQVIVEKLGEPAEGYENFTACLPADECRYAVYDFEYLTEENVPKSRIFFIGWSPDTARVRSKMIYASTKERFKRELDGIQIELQATDPTEMGLDVFKSRAN*