AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0240940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0240940 MtrunA17_Chr2g0291571 98.551 138 2 0 14 151 2 139 2.22e-101 286
Msa0240940 MtrunA17_Chr8g0380291 87.681 138 17 0 14 151 2 139 2.87e-92 263
Msa0240940 MtrunA17_Chr1g0182501 79.710 138 28 0 14 151 2 139 1.71e-83 241
Msa0240940 MtrunA17_Chr8g0386651 71.533 137 37 1 14 150 2 136 6.26e-75 219
Msa0240940 MtrunA17_Chr1g0203211 70.073 137 39 1 14 150 26 160 6.44e-71 210
Msa0240940 MtrunA17_Chr5g0397641 69.343 137 40 1 14 150 2 136 1.53e-70 208
Msa0240940 MtrunA17_Chr7g0261251 57.042 142 61 0 10 151 5 146 4.57e-64 192
Msa0240940 MtrunA17_Chr4g0037251 55.556 135 60 0 16 150 8 142 2.57e-56 172
Msa0240940 MtrunA17_Chr1g0187771 53.793 145 67 0 7 151 3 147 6.55e-52 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0240940 AT5G59880.1 80.435 138 27 0 14 151 2 139 1.92e-85 246
Msa0240940 AT3G46010.2 80.435 138 27 0 14 151 13 150 2.38e-85 247
Msa0240940 AT3G46010.1 80.435 138 27 0 14 151 2 139 3.66e-85 246
Msa0240940 AT5G59890.1 80.435 138 27 0 14 151 2 139 5.15e-83 240
Msa0240940 AT3G46000.1 78.986 138 27 1 14 151 2 137 9.53e-80 232
Msa0240940 AT3G46000.2 78.986 138 27 1 14 151 21 156 1.61e-79 232
Msa0240940 AT5G59890.3 79.545 132 27 0 20 151 1 132 3.01e-78 228
Msa0240940 AT5G59890.2 79.545 132 27 0 20 151 1 132 3.01e-78 228
Msa0240940 AT3G46000.3 78.030 132 27 1 20 151 1 130 5.27e-75 220
Msa0240940 AT4G00680.2 67.606 142 46 0 10 151 8 149 1.22e-74 219
Msa0240940 AT1G01750.2 67.361 144 47 0 8 151 1 144 4.01e-74 218
Msa0240940 AT4G00680.1 68.841 138 43 0 14 151 2 139 2.42e-73 216
Msa0240940 AT1G01750.1 69.565 138 42 0 14 151 2 139 4.14e-73 215
Msa0240940 AT4G25590.1 68.613 137 41 1 14 150 2 136 2.31e-71 211
Msa0240940 AT5G52360.1 69.343 137 40 1 14 150 2 136 3.58e-71 210
Msa0240940 AT5G59880.2 71.014 138 25 1 14 151 2 124 4.05e-71 210
Msa0240940 AT4G25590.2 67.176 131 41 1 20 150 1 129 1.18e-66 199
Msa0240940 AT2G31200.1 52.113 142 68 0 10 151 5 146 4.24e-57 175
Msa0240940 AT4G34970.1 54.015 137 63 0 15 151 5 141 1.31e-55 171
Msa0240940 AT2G16700.1 50.735 136 67 0 16 151 8 143 1.60e-52 163
Msa0240940 AT2G16700.4 50.758 132 65 0 20 151 1 132 1.46e-50 158
Msa0240940 AT2G16700.2 50.758 132 65 0 20 151 1 132 1.46e-50 158
Msa0240940 AT3G45990.1 56.522 138 49 3 20 151 1 133 5.57e-48 152
Msa0240940 AT2G16700.3 50.575 87 43 0 16 102 8 94 5.39e-30 105

Find 78 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACACTTTATGCCACAATTT+TGG 0.126450 2_2:+16394056 None:intergenic
TGACTGCAAGTTAAAGTTTA+TGG 0.155260 2_2:-16394650 Msa0240940:CDS
TCTGAATAGTATAGGCTTTC+TGG 0.160656 2_2:-16394171 Msa0240940:three_prime_UTR
GTTCATGCAAACTCTGTTTA+TGG 0.165538 2_2:-16393780 Msa0240940:three_prime_UTR
CTCCCTCTTGAACCTTTCTT+TGG 0.182021 2_2:+16394283 None:intergenic
CTTCTCACTCTTGCTGTATC+AGG 0.259069 2_2:+16394326 None:intergenic
TAGGCACAGTTGTCACTAAT+TGG 0.290153 2_2:-16393617 Msa0240940:three_prime_UTR
TTCATGCAAACTCTGTTTAT+GGG 0.320236 2_2:-16393779 Msa0240940:three_prime_UTR
GACTAGTGTCAATATTAAAA+AGG 0.322442 2_2:+16393883 None:intergenic
GTAGTGAGATCTTATTATAT+TGG 0.349128 2_2:-16393750 Msa0240940:three_prime_UTR
GAGCTTAGTTGTTGGGAGAA+AGG 0.358954 2_2:-16393680 Msa0240940:three_prime_UTR
CATAAAAGAGCTTAGTTGTT+GGG 0.361793 2_2:-16393687 Msa0240940:three_prime_UTR
GGCAACTGATCCTACTGAAA+TGG 0.363842 2_2:-16394234 Msa0240940:CDS
TGGTCAACTTGACTAGGTTA+TGG 0.369805 2_2:-16394090 Msa0240940:three_prime_UTR
GTTGACCAACCCACAAACTA+TGG 0.374300 2_2:+16394105 None:intergenic
AGGCATTATTTATACTTGAT+AGG 0.377844 2_2:+16393592 None:intergenic
ATTCAGAATCATTATTTAGT+TGG 0.389131 2_2:+16394187 None:intergenic
TGGAATTCAAATAGAGTTGC+AGG 0.390254 2_2:-16394255 Msa0240940:CDS
ATATGACTAACTAAGGCTTA+AGG 0.390867 2_2:+16394136 None:intergenic
TATGACTAACTAAGGCTTAA+GGG 0.394872 2_2:+16394137 None:intergenic
ATAGTCTCACGAATAGAATC+AGG 0.411616 2_2:-16393519 Msa0240940:three_prime_UTR
AAGTGTATTGCACCTTCAAC+AGG 0.412349 2_2:-16394040 Msa0240940:three_prime_UTR
TTAGTGACAACTGTGCCTAA+TGG 0.417440 2_2:+16393621 None:intergenic
TTATGGAGCTCAAGGCAAAA+AGG 0.429370 2_2:-16394633 Msa0240940:CDS
ACAAAACTCAAGCCCGCTAT+GGG 0.449583 2_2:+16393566 None:intergenic
AGTTACAAATTTACCCAGTA+AGG 0.449583 2_2:+16393941 None:intergenic
AACAAAACTCAAGCCCGCTA+TGG 0.467429 2_2:+16393565 None:intergenic
TGTGATGATCAGACAACAAA+TGG 0.468420 2_2:-16393912 Msa0240940:three_prime_UTR
GCAACTGATCCTACTGAAAT+GGG 0.470343 2_2:-16394233 Msa0240940:CDS
CTACAGGCAAATGCAGCATC+TGG 0.471777 2_2:-16394688 Msa0240940:CDS
ATGAAATGACTGAAGTCAAA+AGG 0.475626 2_2:+16393849 None:intergenic
GTTTCTAACTGCAATTCTAC+AGG 0.481385 2_2:-16394704 Msa0240940:CDS
ACTTTAACTTGCAGTCATCA+TGG 0.482180 2_2:+16394656 None:intergenic
TTGAGATATCCATAGTTTGT+GGG 0.483530 2_2:-16394114 Msa0240940:three_prime_UTR
ATAATGATTCTGAATAGTAT+AGG 0.484054 2_2:-16394179 Msa0240940:three_prime_UTR
TATCTCAATATGACTAACTA+AGG 0.495560 2_2:+16394129 None:intergenic
ATAATGCCTTTCCCCATAGC+GGG 0.501178 2_2:-16393578 Msa0240940:three_prime_UTR
GCATAAAAGAGCTTAGTTGT+TGG 0.501561 2_2:-16393688 Msa0240940:three_prime_UTR
GTTAAAGTTTATGGAGCTCA+AGG 0.502426 2_2:-16394641 Msa0240940:CDS
ATTGAGATATCCATAGTTTG+TGG 0.507811 2_2:-16394115 Msa0240940:three_prime_UTR
CGATCTTATACACTATGAAC+CGG 0.509573 2_2:+16394605 None:intergenic
TTTATGCAAGCACCAAAGAA+AGG 0.510363 2_2:-16394295 Msa0240940:CDS
TCGTGAGACTATATTACAAA+AGG 0.513033 2_2:+16393530 None:intergenic
GAGATCTTATTATATTGGTA+TGG 0.514317 2_2:-16393745 Msa0240940:three_prime_UTR
TAGTATAGGCTTTCTGGTGG+GGG 0.515785 2_2:-16394165 Msa0240940:three_prime_UTR
ATGCAATAAATAAAGTACTC+TGG 0.518889 2_2:+16393707 None:intergenic
GAACAAGAGAAGCCTGTTGA+AGG 0.524057 2_2:+16394028 None:intergenic
TTTCTGGTATGGGTATTCAA+AGG 0.528285 2_2:-16393817 Msa0240940:three_prime_UTR
GCATAACGGCACTCATCAGC+AGG 0.530155 2_2:+16394505 None:intergenic
GGCAAATGCAGCATCTGGTA+TGG 0.530905 2_2:-16394683 Msa0240940:CDS
CTCAAGCCCGCTATGGGGAA+AGG 0.536259 2_2:+16393572 None:intergenic
CAAGTCATTGTGGAAAAGCT+TGG 0.539988 2_2:-16394568 Msa0240940:CDS
ATAGTATAGGCTTTCTGGTG+GGG 0.545970 2_2:-16394166 Msa0240940:three_prime_UTR
GTGGGTTGGTCAACTTGACT+AGG 0.547849 2_2:-16394096 Msa0240940:three_prime_UTR
ATGACTAACTAAGGCTTAAG+GGG 0.552433 2_2:+16394138 None:intergenic
AGAAAGGTTCAAGAGGGAGC+TGG 0.559858 2_2:-16394279 Msa0240940:CDS
GAGTGATCAGTTAATCCATT+AGG 0.559965 2_2:-16393636 Msa0240940:three_prime_UTR
GAAACAGAAGCAAGTCATTG+TGG 0.565595 2_2:-16394578 Msa0240940:CDS
GATATCCATAGTTTGTGGGT+TGG 0.569687 2_2:-16394110 Msa0240940:three_prime_UTR
GAATAGTATAGGCTTTCTGG+TGG 0.570109 2_2:-16394168 Msa0240940:three_prime_UTR
GAATCATTATTTAGTTGGCA+CGG 0.570158 2_2:+16394192 None:intergenic
AATAGTATAGGCTTTCTGGT+GGG 0.571857 2_2:-16394167 Msa0240940:three_prime_UTR
GCACCAAAGAAAGGTTCAAG+AGG 0.572284 2_2:-16394286 Msa0240940:CDS
AATAATGCCTTTCCCCATAG+CGG 0.574104 2_2:-16393579 Msa0240940:three_prime_UTR
GTGATGATCAGACAACAAAT+GGG 0.575364 2_2:-16393911 Msa0240940:three_prime_UTR
TGCATGAACAAGACTACAGA+AGG 0.597126 2_2:+16393794 None:intergenic
AGGTTCAAGAGGGAGCTGGA+TGG 0.608161 2_2:-16394275 Msa0240940:CDS
AAGCTTGGTGAGCCAGCTGA+AGG 0.610538 2_2:-16394553 Msa0240940:CDS
CAAAATCATAGACAGCATAA+CGG 0.612434 2_2:+16394491 None:intergenic
ATTAACTGATCACTCACAGC+AGG 0.612608 2_2:+16393644 None:intergenic
ACATCAAGACCCATTTCAGT+AGG 0.621902 2_2:+16394224 None:intergenic
CACCAAAGAAAGGTTCAAGA+GGG 0.636143 2_2:-16394285 Msa0240940:CDS
AACGGCACTCATCAGCAGGA+AGG 0.636804 2_2:+16394509 None:intergenic
TAGTCTCACGAATAGAATCA+GGG 0.637285 2_2:-16393518 Msa0240940:three_prime_UTR
GTTCATAGTGTATAAGATCG+AGG 0.648241 2_2:-16394602 Msa0240940:CDS
CAAATCATAACAAAATACAC+AGG 0.664873 2_2:+16394006 None:intergenic
CAAAACTCAAGCCCGCTATG+GGG 0.686846 2_2:+16393567 None:intergenic
TGATGATCAGACAACAAATG+GGG 0.760183 2_2:-16393910 Msa0240940:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCATTTTTTTGTTTTTC+TGG - chr2_2:16394393-16394412 Msa0240940:intron 15.0%
!!! ATTTCATTTTTTTGTTTTTC+TGG - chr2_2:16394393-16394412 Msa0240940:intron 15.0%
!!! ATGAAAAAAATTCTGCTTTT+GGG + chr2_2:16393784-16393803 None:intergenic 20.0%
!!! AATGAAAAAAATTCTGCTTT+TGG + chr2_2:16393785-16393804 None:intergenic 20.0%
!!! AAAAGCAGAATTTTTTTCAT+TGG - chr2_2:16393784-16393803 Msa0240940:three_prime_UTR 20.0%
!!! AAAGCAGAATTTTTTTCATT+GGG - chr2_2:16393785-16393804 Msa0240940:three_prime_UTR 20.0%
!! ATTCAGAATCATTATTTAGT+TGG + chr2_2:16394042-16394061 None:intergenic 20.0%
!! ATAATGATTCTGAATAGTAT+AGG - chr2_2:16394047-16394066 Msa0240940:three_prime_UTR 20.0%
!!! ATTTTTTTGTTTTTCTGGTA+TGG - chr2_2:16394398-16394417 Msa0240940:intron 20.0%
!!! TTTTTTTGTTTTTCTGGTAT+GGG - chr2_2:16394399-16394418 Msa0240940:intron 20.0%
!!! ATGAAAAAAATTCTGCTTTT+GGG + chr2_2:16393784-16393803 None:intergenic 20.0%
!!! AATGAAAAAAATTCTGCTTT+TGG + chr2_2:16393785-16393804 None:intergenic 20.0%
!!! AAAAGCAGAATTTTTTTCAT+TGG - chr2_2:16393784-16393803 Msa0240940:three_prime_UTR 20.0%
!!! AAAGCAGAATTTTTTTCATT+GGG - chr2_2:16393785-16393804 Msa0240940:three_prime_UTR 20.0%
!! ATTCAGAATCATTATTTAGT+TGG + chr2_2:16394042-16394061 None:intergenic 20.0%
!! ATAATGATTCTGAATAGTAT+AGG - chr2_2:16394047-16394066 Msa0240940:three_prime_UTR 20.0%
!!! ATTTTTTTGTTTTTCTGGTA+TGG - chr2_2:16394398-16394417 Msa0240940:intron 20.0%
!!! TTTTTTTGTTTTTCTGGTAT+GGG - chr2_2:16394399-16394418 Msa0240940:intron 20.0%
!!! TGAAAAAAATTCTGCTTTTG+GGG + chr2_2:16393783-16393802 None:intergenic 25.0%
! TATCTCAATATGACTAACTA+AGG + chr2_2:16394100-16394119 None:intergenic 25.0%
!! TCTCAAATTTTCCAAAATTG+TGG - chr2_2:16394159-16394178 Msa0240940:three_prime_UTR 25.0%
! CAAATCATAACAAAATACAC+AGG + chr2_2:16394223-16394242 None:intergenic 25.0%
!!! CATTTTAGTATATCCTTACT+GGG - chr2_2:16394272-16394291 Msa0240940:CDS 25.0%
! GACTAGTGTCAATATTAAAA+AGG + chr2_2:16394346-16394365 None:intergenic 25.0%
! GTAGTGAGATCTTATTATAT+TGG - chr2_2:16394476-16394495 Msa0240940:CDS 25.0%
! GAGATCTTATTATATTGGTA+TGG - chr2_2:16394481-16394500 Msa0240940:CDS 25.0%
!! ATGCAATAAATAAAGTACTC+TGG + chr2_2:16394522-16394541 None:intergenic 25.0%
! AGGCATTATTTATACTTGAT+AGG + chr2_2:16394637-16394656 None:intergenic 25.0%
!!! TGAAAAAAATTCTGCTTTTG+GGG + chr2_2:16393783-16393802 None:intergenic 25.0%
! TATCTCAATATGACTAACTA+AGG + chr2_2:16394100-16394119 None:intergenic 25.0%
!! TCTCAAATTTTCCAAAATTG+TGG - chr2_2:16394159-16394178 Msa0240940:three_prime_UTR 25.0%
! CAAATCATAACAAAATACAC+AGG + chr2_2:16394223-16394242 None:intergenic 25.0%
!!! CATTTTAGTATATCCTTACT+GGG - chr2_2:16394272-16394291 Msa0240940:CDS 25.0%
! GACTAGTGTCAATATTAAAA+AGG + chr2_2:16394346-16394365 None:intergenic 25.0%
! GTAGTGAGATCTTATTATAT+TGG - chr2_2:16394476-16394495 Msa0240940:CDS 25.0%
! GAGATCTTATTATATTGGTA+TGG - chr2_2:16394481-16394500 Msa0240940:CDS 25.0%
!! ATGCAATAAATAAAGTACTC+TGG + chr2_2:16394522-16394541 None:intergenic 25.0%
! AGGCATTATTTATACTTGAT+AGG + chr2_2:16394637-16394656 None:intergenic 25.0%
TGACTGCAAGTTAAAGTTTA+TGG - chr2_2:16393576-16393595 Msa0240940:three_prime_UTR 30.0%
CAAAATCATAGACAGCATAA+CGG + chr2_2:16393738-16393757 None:intergenic 30.0%
!! AATGTTTGCTTTTCGATACA+TGG - chr2_2:16393812-16393831 Msa0240940:three_prime_UTR 30.0%
GAATCATTATTTAGTTGGCA+CGG + chr2_2:16394037-16394056 None:intergenic 30.0%
TATGACTAACTAAGGCTTAA+GGG + chr2_2:16394092-16394111 None:intergenic 30.0%
ATATGACTAACTAAGGCTTA+AGG + chr2_2:16394093-16394112 None:intergenic 30.0%
ATTGAGATATCCATAGTTTG+TGG - chr2_2:16394111-16394130 Msa0240940:three_prime_UTR 30.0%
! TTGAGATATCCATAGTTTGT+GGG - chr2_2:16394112-16394131 Msa0240940:three_prime_UTR 30.0%
! TACACTTTATGCCACAATTT+TGG + chr2_2:16394173-16394192 None:intergenic 30.0%
! ATACAATCTTTTCGTTTGAG+TGG - chr2_2:16394249-16394268 Msa0240940:CDS 30.0%
!! GCATTTTAGTATATCCTTAC+TGG - chr2_2:16394271-16394290 Msa0240940:CDS 30.0%
AGTTACAAATTTACCCAGTA+AGG + chr2_2:16394288-16394307 None:intergenic 30.0%
ATGAAATGACTGAAGTCAAA+AGG + chr2_2:16394380-16394399 None:intergenic 30.0%
TTCATGCAAACTCTGTTTAT+GGG - chr2_2:16394447-16394466 Msa0240940:CDS 30.0%
CATAAAAGAGCTTAGTTGTT+GGG - chr2_2:16394539-16394558 Msa0240940:CDS 30.0%
TCGTGAGACTATATTACAAA+AGG + chr2_2:16394699-16394718 None:intergenic 30.0%
TGACTGCAAGTTAAAGTTTA+TGG - chr2_2:16393576-16393595 Msa0240940:three_prime_UTR 30.0%
CAAAATCATAGACAGCATAA+CGG + chr2_2:16393738-16393757 None:intergenic 30.0%
!! AATGTTTGCTTTTCGATACA+TGG - chr2_2:16393812-16393831 Msa0240940:three_prime_UTR 30.0%
GAATCATTATTTAGTTGGCA+CGG + chr2_2:16394037-16394056 None:intergenic 30.0%
TATGACTAACTAAGGCTTAA+GGG + chr2_2:16394092-16394111 None:intergenic 30.0%
ATATGACTAACTAAGGCTTA+AGG + chr2_2:16394093-16394112 None:intergenic 30.0%
ATTGAGATATCCATAGTTTG+TGG - chr2_2:16394111-16394130 Msa0240940:three_prime_UTR 30.0%
! TTGAGATATCCATAGTTTGT+GGG - chr2_2:16394112-16394131 Msa0240940:three_prime_UTR 30.0%
! TACACTTTATGCCACAATTT+TGG + chr2_2:16394173-16394192 None:intergenic 30.0%
! ATACAATCTTTTCGTTTGAG+TGG - chr2_2:16394249-16394268 Msa0240940:CDS 30.0%
!! GCATTTTAGTATATCCTTAC+TGG - chr2_2:16394271-16394290 Msa0240940:CDS 30.0%
AGTTACAAATTTACCCAGTA+AGG + chr2_2:16394288-16394307 None:intergenic 30.0%
ATGAAATGACTGAAGTCAAA+AGG + chr2_2:16394380-16394399 None:intergenic 30.0%
TTCATGCAAACTCTGTTTAT+GGG - chr2_2:16394447-16394466 Msa0240940:CDS 30.0%
CATAAAAGAGCTTAGTTGTT+GGG - chr2_2:16394539-16394558 Msa0240940:CDS 30.0%
TCGTGAGACTATATTACAAA+AGG + chr2_2:16394699-16394718 None:intergenic 30.0%
GTTTCTAACTGCAATTCTAC+AGG - chr2_2:16393522-16393541 Msa0240940:three_prime_UTR 35.0%
!! ACTTTAACTTGCAGTCATCA+TGG + chr2_2:16393573-16393592 None:intergenic 35.0%
GTTAAAGTTTATGGAGCTCA+AGG - chr2_2:16393585-16393604 Msa0240940:three_prime_UTR 35.0%
CGATCTTATACACTATGAAC+CGG + chr2_2:16393624-16393643 None:intergenic 35.0%
GTTCATAGTGTATAAGATCG+AGG - chr2_2:16393624-16393643 Msa0240940:three_prime_UTR 35.0%
! AAATTTTCATAGCCTTCAGC+TGG + chr2_2:16393688-16393707 None:intergenic 35.0%
!!! GCAGAATTTTTTTCATTGGG+TGG - chr2_2:16393788-16393807 Msa0240940:three_prime_UTR 35.0%
! TACATGGCTATAGTTTTCCT+TGG - chr2_2:16393828-16393847 Msa0240940:three_prime_UTR 35.0%
AGATAGGAAGTAAACAACCA+AGG + chr2_2:16393848-16393867 None:intergenic 35.0%
CCAACAGAATTAATGCAGAT+AGG + chr2_2:16393864-16393883 None:intergenic 35.0%
CCTATCTGCATTAATTCTGT+TGG - chr2_2:16393861-16393880 Msa0240940:three_prime_UTR 35.0%
TTTATGCAAGCACCAAAGAA+AGG - chr2_2:16393931-16393950 Msa0240940:three_prime_UTR 35.0%
TGGAATTCAAATAGAGTTGC+AGG - chr2_2:16393971-16393990 Msa0240940:three_prime_UTR 35.0%
TCTGAATAGTATAGGCTTTC+TGG - chr2_2:16394055-16394074 Msa0240940:three_prime_UTR 35.0%
! AATAGTATAGGCTTTCTGGT+GGG - chr2_2:16394059-16394078 Msa0240940:three_prime_UTR 35.0%
ATGACTAACTAAGGCTTAAG+GGG + chr2_2:16394091-16394110 None:intergenic 35.0%
TGTGATGATCAGACAACAAA+TGG - chr2_2:16394314-16394333 Msa0240940:CDS 35.0%
GTGATGATCAGACAACAAAT+GGG - chr2_2:16394315-16394334 Msa0240940:CDS 35.0%
TGATGATCAGACAACAAATG+GGG - chr2_2:16394316-16394335 Msa0240940:CDS 35.0%
! TTTCTGGTATGGGTATTCAA+AGG - chr2_2:16394409-16394428 Msa0240940:intron 35.0%
GTTCATGCAAACTCTGTTTA+TGG - chr2_2:16394446-16394465 Msa0240940:CDS 35.0%
GCATAAAAGAGCTTAGTTGT+TGG - chr2_2:16394538-16394557 Msa0240940:CDS 35.0%
GAGTGATCAGTTAATCCATT+AGG - chr2_2:16394590-16394609 Msa0240940:CDS 35.0%
ATAGTCTCACGAATAGAATC+AGG - chr2_2:16394707-16394726 Msa0240940:CDS 35.0%
TAGTCTCACGAATAGAATCA+GGG - chr2_2:16394708-16394727 Msa0240940:CDS 35.0%
GTTTCTAACTGCAATTCTAC+AGG - chr2_2:16393522-16393541 Msa0240940:three_prime_UTR 35.0%
!! ACTTTAACTTGCAGTCATCA+TGG + chr2_2:16393573-16393592 None:intergenic 35.0%
GTTAAAGTTTATGGAGCTCA+AGG - chr2_2:16393585-16393604 Msa0240940:three_prime_UTR 35.0%
CGATCTTATACACTATGAAC+CGG + chr2_2:16393624-16393643 None:intergenic 35.0%
GTTCATAGTGTATAAGATCG+AGG - chr2_2:16393624-16393643 Msa0240940:three_prime_UTR 35.0%
! AAATTTTCATAGCCTTCAGC+TGG + chr2_2:16393688-16393707 None:intergenic 35.0%
!!! GCAGAATTTTTTTCATTGGG+TGG - chr2_2:16393788-16393807 Msa0240940:three_prime_UTR 35.0%
! TACATGGCTATAGTTTTCCT+TGG - chr2_2:16393828-16393847 Msa0240940:three_prime_UTR 35.0%
AGATAGGAAGTAAACAACCA+AGG + chr2_2:16393848-16393867 None:intergenic 35.0%
CCAACAGAATTAATGCAGAT+AGG + chr2_2:16393864-16393883 None:intergenic 35.0%
CCTATCTGCATTAATTCTGT+TGG - chr2_2:16393861-16393880 Msa0240940:three_prime_UTR 35.0%
TTTATGCAAGCACCAAAGAA+AGG - chr2_2:16393931-16393950 Msa0240940:three_prime_UTR 35.0%
TGGAATTCAAATAGAGTTGC+AGG - chr2_2:16393971-16393990 Msa0240940:three_prime_UTR 35.0%
TCTGAATAGTATAGGCTTTC+TGG - chr2_2:16394055-16394074 Msa0240940:three_prime_UTR 35.0%
! AATAGTATAGGCTTTCTGGT+GGG - chr2_2:16394059-16394078 Msa0240940:three_prime_UTR 35.0%
ATGACTAACTAAGGCTTAAG+GGG + chr2_2:16394091-16394110 None:intergenic 35.0%
TGTGATGATCAGACAACAAA+TGG - chr2_2:16394314-16394333 Msa0240940:CDS 35.0%
GTGATGATCAGACAACAAAT+GGG - chr2_2:16394315-16394334 Msa0240940:CDS 35.0%
TGATGATCAGACAACAAATG+GGG - chr2_2:16394316-16394335 Msa0240940:CDS 35.0%
! TTTCTGGTATGGGTATTCAA+AGG - chr2_2:16394409-16394428 Msa0240940:intron 35.0%
GTTCATGCAAACTCTGTTTA+TGG - chr2_2:16394446-16394465 Msa0240940:CDS 35.0%
GCATAAAAGAGCTTAGTTGT+TGG - chr2_2:16394538-16394557 Msa0240940:CDS 35.0%
GAGTGATCAGTTAATCCATT+AGG - chr2_2:16394590-16394609 Msa0240940:CDS 35.0%
ATAGTCTCACGAATAGAATC+AGG - chr2_2:16394707-16394726 Msa0240940:CDS 35.0%
TAGTCTCACGAATAGAATCA+GGG - chr2_2:16394708-16394727 Msa0240940:CDS 35.0%
TTATGGAGCTCAAGGCAAAA+AGG - chr2_2:16393593-16393612 Msa0240940:three_prime_UTR 40.0%
GAAACAGAAGCAAGTCATTG+TGG - chr2_2:16393648-16393667 Msa0240940:three_prime_UTR 40.0%
CAAGTCATTGTGGAAAAGCT+TGG - chr2_2:16393658-16393677 Msa0240940:three_prime_UTR 40.0%
!! ATTCTGTTGGCTTTGTGTGT+AGG - chr2_2:16393874-16393893 Msa0240940:three_prime_UTR 40.0%
CACCAAAGAAAGGTTCAAGA+GGG - chr2_2:16393941-16393960 Msa0240940:three_prime_UTR 40.0%
GCAACTGATCCTACTGAAAT+GGG - chr2_2:16393993-16394012 Msa0240940:three_prime_UTR 40.0%
ACATCAAGACCCATTTCAGT+AGG + chr2_2:16394005-16394024 None:intergenic 40.0%
GAATAGTATAGGCTTTCTGG+TGG - chr2_2:16394058-16394077 Msa0240940:three_prime_UTR 40.0%
! ATAGTATAGGCTTTCTGGTG+GGG - chr2_2:16394060-16394079 Msa0240940:three_prime_UTR 40.0%
! GATATCCATAGTTTGTGGGT+TGG - chr2_2:16394116-16394135 Msa0240940:three_prime_UTR 40.0%
TGGTCAACTTGACTAGGTTA+TGG - chr2_2:16394136-16394155 Msa0240940:three_prime_UTR 40.0%
AAGTGTATTGCACCTTCAAC+AGG - chr2_2:16394186-16394205 Msa0240940:exon 40.0%
TGCATGAACAAGACTACAGA+AGG + chr2_2:16394435-16394454 None:intergenic 40.0%
ATTAACTGATCACTCACAGC+AGG + chr2_2:16394585-16394604 None:intergenic 40.0%
TTAGTGACAACTGTGCCTAA+TGG + chr2_2:16394608-16394627 None:intergenic 40.0%
TAGGCACAGTTGTCACTAAT+TGG - chr2_2:16394609-16394628 Msa0240940:CDS 40.0%
AATAATGCCTTTCCCCATAG+CGG - chr2_2:16394647-16394666 Msa0240940:CDS 40.0%
TTATGGAGCTCAAGGCAAAA+AGG - chr2_2:16393593-16393612 Msa0240940:three_prime_UTR 40.0%
GAAACAGAAGCAAGTCATTG+TGG - chr2_2:16393648-16393667 Msa0240940:three_prime_UTR 40.0%
CAAGTCATTGTGGAAAAGCT+TGG - chr2_2:16393658-16393677 Msa0240940:three_prime_UTR 40.0%
!! ATTCTGTTGGCTTTGTGTGT+AGG - chr2_2:16393874-16393893 Msa0240940:three_prime_UTR 40.0%
CACCAAAGAAAGGTTCAAGA+GGG - chr2_2:16393941-16393960 Msa0240940:three_prime_UTR 40.0%
GCAACTGATCCTACTGAAAT+GGG - chr2_2:16393993-16394012 Msa0240940:three_prime_UTR 40.0%
ACATCAAGACCCATTTCAGT+AGG + chr2_2:16394005-16394024 None:intergenic 40.0%
GAATAGTATAGGCTTTCTGG+TGG - chr2_2:16394058-16394077 Msa0240940:three_prime_UTR 40.0%
! ATAGTATAGGCTTTCTGGTG+GGG - chr2_2:16394060-16394079 Msa0240940:three_prime_UTR 40.0%
! GATATCCATAGTTTGTGGGT+TGG - chr2_2:16394116-16394135 Msa0240940:three_prime_UTR 40.0%
TGGTCAACTTGACTAGGTTA+TGG - chr2_2:16394136-16394155 Msa0240940:three_prime_UTR 40.0%
AAGTGTATTGCACCTTCAAC+AGG - chr2_2:16394186-16394205 Msa0240940:exon 40.0%
TGCATGAACAAGACTACAGA+AGG + chr2_2:16394435-16394454 None:intergenic 40.0%
ATTAACTGATCACTCACAGC+AGG + chr2_2:16394585-16394604 None:intergenic 40.0%
TTAGTGACAACTGTGCCTAA+TGG + chr2_2:16394608-16394627 None:intergenic 40.0%
TAGGCACAGTTGTCACTAAT+TGG - chr2_2:16394609-16394628 Msa0240940:CDS 40.0%
AATAATGCCTTTCCCCATAG+CGG - chr2_2:16394647-16394666 Msa0240940:CDS 40.0%
TCAAGGCAAAAAGGACACAC+CGG - chr2_2:16393602-16393621 Msa0240940:three_prime_UTR 45.0%
! CTTCTCACTCTTGCTGTATC+AGG + chr2_2:16393903-16393922 None:intergenic 45.0%
GCACCAAAGAAAGGTTCAAG+AGG - chr2_2:16393940-16393959 Msa0240940:three_prime_UTR 45.0%
CTCCCTCTTGAACCTTTCTT+TGG + chr2_2:16393946-16393965 None:intergenic 45.0%
GGCAACTGATCCTACTGAAA+TGG - chr2_2:16393992-16394011 Msa0240940:three_prime_UTR 45.0%
! TAGTATAGGCTTTCTGGTGG+GGG - chr2_2:16394061-16394080 Msa0240940:three_prime_UTR 45.0%
GTTGACCAACCCACAAACTA+TGG + chr2_2:16394124-16394143 None:intergenic 45.0%
GAACAAGAGAAGCCTGTTGA+AGG + chr2_2:16394201-16394220 None:intergenic 45.0%
GAGCTTAGTTGTTGGGAGAA+AGG - chr2_2:16394546-16394565 Msa0240940:CDS 45.0%
ATAATGCCTTTCCCCATAGC+GGG - chr2_2:16394648-16394667 Msa0240940:CDS 45.0%
ACAAAACTCAAGCCCGCTAT+GGG + chr2_2:16394663-16394682 None:intergenic 45.0%
AACAAAACTCAAGCCCGCTA+TGG + chr2_2:16394664-16394683 None:intergenic 45.0%
TCAAGGCAAAAAGGACACAC+CGG - chr2_2:16393602-16393621 Msa0240940:three_prime_UTR 45.0%
! CTTCTCACTCTTGCTGTATC+AGG + chr2_2:16393903-16393922 None:intergenic 45.0%
GCACCAAAGAAAGGTTCAAG+AGG - chr2_2:16393940-16393959 Msa0240940:three_prime_UTR 45.0%
CTCCCTCTTGAACCTTTCTT+TGG + chr2_2:16393946-16393965 None:intergenic 45.0%
GGCAACTGATCCTACTGAAA+TGG - chr2_2:16393992-16394011 Msa0240940:three_prime_UTR 45.0%
! TAGTATAGGCTTTCTGGTGG+GGG - chr2_2:16394061-16394080 Msa0240940:three_prime_UTR 45.0%
GTTGACCAACCCACAAACTA+TGG + chr2_2:16394124-16394143 None:intergenic 45.0%
GAACAAGAGAAGCCTGTTGA+AGG + chr2_2:16394201-16394220 None:intergenic 45.0%
GAGCTTAGTTGTTGGGAGAA+AGG - chr2_2:16394546-16394565 Msa0240940:CDS 45.0%
ATAATGCCTTTCCCCATAGC+GGG - chr2_2:16394648-16394667 Msa0240940:CDS 45.0%
ACAAAACTCAAGCCCGCTAT+GGG + chr2_2:16394663-16394682 None:intergenic 45.0%
AACAAAACTCAAGCCCGCTA+TGG + chr2_2:16394664-16394683 None:intergenic 45.0%
CTACAGGCAAATGCAGCATC+TGG - chr2_2:16393538-16393557 Msa0240940:three_prime_UTR 50.0%
GGCAAATGCAGCATCTGGTA+TGG - chr2_2:16393543-16393562 Msa0240940:three_prime_UTR 50.0%
AGAAAGGTTCAAGAGGGAGC+TGG - chr2_2:16393947-16393966 Msa0240940:three_prime_UTR 50.0%
!! GTGGGTTGGTCAACTTGACT+AGG - chr2_2:16394130-16394149 Msa0240940:three_prime_UTR 50.0%
CAAAACTCAAGCCCGCTATG+GGG + chr2_2:16394662-16394681 None:intergenic 50.0%
CTACAGGCAAATGCAGCATC+TGG - chr2_2:16393538-16393557 Msa0240940:three_prime_UTR 50.0%
GGCAAATGCAGCATCTGGTA+TGG - chr2_2:16393543-16393562 Msa0240940:three_prime_UTR 50.0%
AGAAAGGTTCAAGAGGGAGC+TGG - chr2_2:16393947-16393966 Msa0240940:three_prime_UTR 50.0%
!! GTGGGTTGGTCAACTTGACT+AGG - chr2_2:16394130-16394149 Msa0240940:three_prime_UTR 50.0%
CAAAACTCAAGCCCGCTATG+GGG + chr2_2:16394662-16394681 None:intergenic 50.0%
! AAGCTTGGTGAGCCAGCTGA+AGG - chr2_2:16393673-16393692 Msa0240940:three_prime_UTR 55.0%
AACGGCACTCATCAGCAGGA+AGG + chr2_2:16393720-16393739 None:intergenic 55.0%
GCATAACGGCACTCATCAGC+AGG + chr2_2:16393724-16393743 None:intergenic 55.0%
AGGTTCAAGAGGGAGCTGGA+TGG - chr2_2:16393951-16393970 Msa0240940:three_prime_UTR 55.0%
! AAGCTTGGTGAGCCAGCTGA+AGG - chr2_2:16393673-16393692 Msa0240940:three_prime_UTR 55.0%
AACGGCACTCATCAGCAGGA+AGG + chr2_2:16393720-16393739 None:intergenic 55.0%
GCATAACGGCACTCATCAGC+AGG + chr2_2:16393724-16393743 None:intergenic 55.0%
AGGTTCAAGAGGGAGCTGGA+TGG - chr2_2:16393951-16393970 Msa0240940:three_prime_UTR 55.0%
CTCAAGCCCGCTATGGGGAA+AGG + chr2_2:16394657-16394676 None:intergenic 60.0%
CTCAAGCCCGCTATGGGGAA+AGG + chr2_2:16394657-16394676 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 16393505 16394743 16393505 ID=Msa0240940;Name=Msa0240940
chr2_2 mRNA 16393505 16394743 16393505 ID=Msa0240940-mRNA-1;Parent=Msa0240940;Name=Msa0240940-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|1|0.5|1|1|1|2|697|151
chr2_2 exon 16393505 16394352 16393505 ID=Msa0240940-mRNA-1:exon:6729;Parent=Msa0240940-mRNA-1
chr2_2 exon 16394439 16394743 16394439 ID=Msa0240940-mRNA-1:exon:6728;Parent=Msa0240940-mRNA-1
chr2_2 CDS 16394439 16394743 16394439 ID=Msa0240940-mRNA-1:cds;Parent=Msa0240940-mRNA-1
chr2_2 CDS 16394202 16394352 16394202 ID=Msa0240940-mRNA-1:cds;Parent=Msa0240940-mRNA-1
chr2_2 three_prime_UTR 16393505 16394201 16393505 ID=Msa0240940-mRNA-1:three_prime_utr;Parent=Msa0240940-mRNA-1
Gene Sequence

>Msa0240940

ATGATTGATTATGATGTGTTTCTAACTGCAATTCTACAGGCAAATGCAGCATCTGGTATGGCAGTCCATGATGACTGCAAGTTAAAGTTTATGGAGCTCAAGGCAAAAAGGACACACCGGTTCATAGTGTATAAGATCGAGGAGAAACAGAAGCAAGTCATTGTGGAAAAGCTTGGTGAGCCAGCTGAAGGCTATGAAAATTTCACTGCATGCCTTCCTGCTGATGAGTGCCGTTATGCTGTCTATGATTTTGAGTATCTGACAGAAGAGAATGTCCCCAAAAGCAGAATTTTTTTCATTGGGTGGTCGCCTGATACAGCAAGAGTGAGAAGCAAGATGATTTATGCAAGCACCAAAGAAAGGTTCAAGAGGGAGCTGGATGGAATTCAAATAGAGTTGCAGGCAACTGATCCTACTGAAATGGGTCTTGATGTGTTCAAAAGCCGTGCCAACTAA
Protein sequence

>Msa0240940

MIDYDVFLTAILQANAASGMAVHDDCKLKFMELKAKRTHRFIVYKIEEKQKQVIVEKLGEPAEGYENFTACLPADECRYAVYDFEYLTEENVPKSRIFFIGWSPDTARVRSKMIYASTKERFKRELDGIQIELQATDPTEMGLDVFKSRAN*