AlfalfaGEDB | Msa0319500

M. sativa cultivar zhongmu-4 / Msa0319500

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0319500	XP_003594704.1	96.847	222	7	0	1	222	1	222	1.72e-152	432

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0319500	Msa1031180	0.842540	2.430222e-58	-8.615850e-47
Msa0319500	Msa1070360	0.824156	9.403442e-54	-8.615850e-47
Msa0319500	Msa1119430	0.833182	6.176155e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0319500	MtrunA17_Chr2g0294131	96.847	222	7	0	1	222	1	222	1.58e-156	432
Msa0319500	MtrunA17_Chr4g0068671	53.252	246	86	7	1	222	1	241	2.51e-47	156
Msa0319500	MtrunA17_Chr1g0194401	45.455	154	81	3	51	203	29	180	9.26e-35	123

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0319500	AT3G17120.4	40.639	219	98	5	6	211	2	201	2.16e-32	117
Msa0319500	AT3G17120.2	40.639	219	98	5	6	211	2	201	2.16e-32	117
Msa0319500	AT3G17120.1	40.639	219	98	5	6	211	2	201	2.16e-32	117
Msa0319500	AT3G17120.5	40.639	219	98	5	6	211	2	201	2.16e-32	117
Msa0319500	AT3G17120.3	40.639	219	98	5	6	211	2	201	2.16e-32	117
Msa0319500	AT1G02380.1	37.445	227	126	9	1	216	1	222	1.10e-30	113
Msa0319500	AT4G01960.1	35.000	240	130	10	1	222	1	232	3.52e-22	91.3
Msa0319500	AT4G01960.2	35.000	240	130	10	1	222	1	232	3.52e-22	91.3
Msa0319500	AT4G01960.3	38.286	175	87	6	1	159	1	170	8.38e-19	81.3

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Find 87 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTCCGCGACCTCCACA+AGG	0.611809	2_4:-19253225	Msa0319500:CDS
AAAGGTGGGAGAATTTGGAT+TGG	0.504466	2_4:-19253519	Msa0319500:five_prime_UTR
AACAGAGGAGGGTTTGATTT+CGG	0.332157	2_4:-19252304	Msa0319500:CDS
AAGATAGGAAAGAGGTGTGA+CGG	0.553834	2_4:+19254915	None:intergenic
AATCACTTGATGGATTGAGT+TGG	0.614440	2_4:+19252274	None:intergenic
ACCTCCACAAGGCCCGTCCT+TGG	0.439286	2_4:-19253214	Msa0319500:CDS
AGAATCTCTATGATTTCTAG+TGG	0.517852	2_4:-19253399	Msa0319500:CDS
AGAGAGGGAGAGGGAAAAGA+AGG	0.369582	2_4:+19254761	None:intergenic
AGATTTGATTGAGAAAAGAT+AGG	0.439239	2_4:+19254900	None:intergenic
AGCAGATCATAAACTAATAA+AGG	0.356004	2_4:-19253187	Msa0319500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTATCTATGACATTACAGT+TGG	+	chr2_4:19251641-19251660	None:intergenic	30.0%
ATTGAGAAAAGATAGGAAAG+AGG	+	chr2_4:19251677-19251696	None:intergenic	30.0%
AACCCTCAAATGATGATAAT+GGG	+	chr2_4:19251973-19251992	None:intergenic	30.0%
TAACCCTCAAATGATGATAA+TGG	+	chr2_4:19251974-19251993	None:intergenic	30.0%
TACCCCAAATGAAGATAATT+GGG	+	chr2_4:19252149-19252168	None:intergenic	30.0%
ATACCCCAAATGAAGATAAT+TGG	+	chr2_4:19252150-19252169	None:intergenic	30.0%
CCAAAGATAAAACCCATTAA+AGG	-	chr2_4:19252654-19252673	Msa0319500:intron	30.0%
TGATGGTTTATGATCGATTA+AGG	-	chr2_4:19252935-19252954	Msa0319500:intron	30.0%
TAATAAAAGGTGGGAGAATT+TGG	-	chr2_4:19253057-19253076	Msa0319500:CDS	30.0%
CTGTTAATATGATGAAAGTG+GGG	-	chr2_4:19253279-19253298	Msa0319500:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	19251626	19254977	19251626	ID=Msa0319500;Name=Msa0319500
chr2_4	mRNA	19251626	19254977	19251626	ID=Msa0319500-mRNA-1;Parent=Msa0319500;Name=Msa0319500-mRNA-1;_AED=0.12;_eAED=0.12;_QI=501\|1\|1\|1\|0.66\|0.5\|4\|366\|224
chr2_4	exon	19251626	19252046	19251626	ID=Msa0319500-mRNA-1:exon:7132;Parent=Msa0319500-mRNA-1
chr2_4	exon	19252246	19252390	19252246	ID=Msa0319500-mRNA-1:exon:7131;Parent=Msa0319500-mRNA-1
chr2_4	exon	19253028	19253711	19253028	ID=Msa0319500-mRNA-1:exon:7130;Parent=Msa0319500-mRNA-1
chr2_4	exon	19254686	19254977	19254686	ID=Msa0319500-mRNA-1:exon:7129;Parent=Msa0319500-mRNA-1
chr2_4	five_prime_UTR	19254686	19254977	19254686	ID=Msa0319500-mRNA-1:five_prime_utr;Parent=Msa0319500-mRNA-1
chr2_4	five_prime_UTR	19253503	19253711	19253503	ID=Msa0319500-mRNA-1:five_prime_utr;Parent=Msa0319500-mRNA-1
chr2_4	CDS	19253028	19253502	19253028	ID=Msa0319500-mRNA-1:cds;Parent=Msa0319500-mRNA-1
chr2_4	CDS	19252246	19252390	19252246	ID=Msa0319500-mRNA-1:cds;Parent=Msa0319500-mRNA-1
chr2_4	CDS	19251992	19252046	19251992	ID=Msa0319500-mRNA-1:cds;Parent=Msa0319500-mRNA-1
chr2_4	three_prime_UTR	19251626	19251991	19251626	ID=Msa0319500-mRNA-1:three_prime_utr;Parent=Msa0319500-mRNA-1

Gene Sequence

>Msa0319500

ATGGATCAAAGAAATGGAAGGGAGAAAGATATTGAAGTTGATATTGAAAGTGGATTGCCACTTATCGAAGATAACTCAAGGAGAATCTCTATGATTTCTAGTGGATTTGTTGGAGGTTCTGTTAATGGCGAAGCCAGGCCAAGTTCTTATTTGAATGAATCAAATTTAAGTGAAGTTTCTGTTAATATGATGAAAGTGGGGAACAAGGCTCCAGCAAAAGAAAAACGGAAAAAGGCGTCGGGTAAAAAGGCTGCCAAACCTCCGCGACCTCCACAAGGCCCGTCCTTGGACGCAGCAGATCATAAACTAATAAAGGAGATGTCTGAACTTGCGATGTTGAAGCGTGCAAGGATAGAGCGTATGAAAGCATTGAAGAAAATGAGAACTGCGAAATCGTCGTCGCCGTCATCTAATAGCAGCAGCATTGTGGCTATGATTTTCACTGTTATCTTCTTCATTGTGATAATCTGCCAAGGCATGTCATCAACTGGAAAAAGCTCAGTGTCAAGTTTCCAAGGATCACCTGTATCAGCAGGCGGAACAGAGGAGGGTTTGATTTCGGTTCAGTACCAACTCAATCCATCAAGTGATTCAAATGCGCCAGGGTCGGAGTCCATCAATTTTGTACAGCGGGTATCAGGTTCAGATTTGCACGAAAAACTGAGGACAGGTTGA

Protein sequence

>Msa0319500

MDQRNGREKDIEVDIESGLPLIEDNSRRISMISSGFVGGSVNGEARPSSYLNESNLSEVSVNMMKVGNKAPAKEKRKKASGKKAAKPPRPPQGPSLDAADHKLIKEMSELAMLKRARIERMKALKKMRTAKSSSPSSNSSSIVAMIFTVIFFIVIICQGMSSTGKSSVSSFQGSPVSAGGTEEGLISVQYQLNPSSDSNAPGSESINFVQRVSGSDLHEKLRTG*