AlfalfaGEDB | Msa0320990

M. sativa cultivar zhongmu-4 / Msa0320990

KEGG: -
GO: -
PFAM: PF03476.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0320990	XP_003594861.1	83.168	303	13	1	26	290	1	303	1.97e-180	510

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0320990	sp\|O88994\|MARC2_RAT	32.759	232	134	5	37	258	55	274	5.33e-32	124

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0320990	G7INC8	83.168	303	13	1	26	290	1	303	0.0	510

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0282170	Msa0320990	0.806135	9.529009e-50	-8.615850e-47
Msa0320990	Msa1450020	0.803003	4.288704e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0320990	MtrunA17_Chr2g0296871	83.168	303	13	1	26	290	1	303	0.0	510
Msa0320990	MtrunA17_Chr2g0296881	76.221	307	31	2	26	290	1	307	2.84e-169	471
Msa0320990	MtrunA17_Chr2g0296891	74.747	297	37	1	36	294	20	316	1.59e-163	457
Msa0320990	MtrunA17_Chr4g0013751	29.482	251	144	8	39	269	518	755	2.28e-24	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0320990	AT1G30910.1	54.392	296	95	3	32	290	14	306	4.31e-113	330
Msa0320990	AT5G44720.1	50.000	292	106	3	38	290	6	296	7.60e-100	295
Msa0320990	AT5G44720.2	59.641	223	89	1	38	260	6	227	1.51e-96	284
Msa0320990	AT1G16540.1	26.250	240	149	5	39	260	531	760	4.74e-24	102
Msa0320990	AT1G16540.3	25.692	253	157	5	39	260	531	783	1.82e-22	97.8
Msa0320990	AT1G16540.2	23.913	184	122	4	39	214	531	704	2.05e-11	65.1

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Find 171 sgRNAs with CRISPR-Local

Find 1053 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTAAAATACCCATAA+AGG	0.377071	2_4:+22004799	None:intergenic
AAACAGGATCTCCCAATTTA+AGG	0.238470	2_4:+21993188	None:intergenic
AAACAGGATCTCCCAATTTA+AGG	0.238470	2_4:+22001253	None:intergenic
AAACATCACATTTCACATGA+GGG	0.586272	2_4:+21996805	None:intergenic
AAACTGGTCCGCTTCAATAC+TGG	0.403839	2_4:-21994293	Msa0320990:intron
AAACTGGTTCGCTTCAATAC+TGG	0.446407	2_4:-22002764	Msa0320990:intron
AAATACATGAGTAAAATGAT+TGG	0.497598	2_4:+21992885	None:intergenic
AAATGTGATGTTTAAAGTTT+GGG	0.364719	2_4:-21996795	Msa0320990:exon
AACATCACATTTCACATGAG+GGG	0.585000	2_4:+21996806	None:intergenic
AACCATATTCTGAAGACCTA+TGG	0.482705	2_4:-22002130	Msa0320990:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GCAGACTTAAACAGAAAATT+TGG	-	chr2_4:21993152-21993171	Msa0320990:three_prime_UTR	30.0%
CTCGCCTAACTATAAAAATT+GGG	-	chr2_4:21993312-21993331	Msa0320990:intron	30.0%
TCGCCTAACTATAAAAATTG+GGG	-	chr2_4:21993313-21993332	Msa0320990:intron	30.0%
ACACTATATAGAAGTTGTAG+AGG	-	chr2_4:21993532-21993551	Msa0320990:intron	30.0%
GAATGTTGTTGCAACATTTA+AGG	+	chr2_4:21993786-21993805	None:intergenic	30.0%
ATGTTGCAACAACATTCTTA+AGG	-	chr2_4:21993789-21993808	Msa0320990:intron	30.0%
GACATAATGTCTTAACATGT+TGG	+	chr2_4:21993969-21993988	None:intergenic	30.0%
TCAGTCATAACCTAATTGTT+AGG	+	chr2_4:21994048-21994067	None:intergenic	30.0%
GGTTATGACTGATAGATTAA+CGG	-	chr2_4:21994056-21994075	Msa0320990:three_prime_UTR	30.0%
CGAATTCACTTAAGAGATTA+AGG	-	chr2_4:21994102-21994121	Msa0320990:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	21992782	22010512	21992782	ID=Msa0320990;Name=Msa0320990
chr2_4	mRNA	21992782	22010512	21992782	ID=Msa0320990-mRNA-1;Parent=Msa0320990;Name=Msa0320990-mRNA-1;_AED=0.28;_eAED=0.29;_QI=0\|0.8\|0.75\|1\|0.8\|0.68\|16\|1420\|298
chr2_4	exon	21992782	21993275	21992782	ID=Msa0320990-mRNA-1:exon:8100;Parent=Msa0320990-mRNA-1
chr2_4	exon	21993362	21993472	21993362	ID=Msa0320990-mRNA-1:exon:8099;Parent=Msa0320990-mRNA-1
chr2_4	exon	21993662	21993764	21993662	ID=Msa0320990-mRNA-1:exon:8098;Parent=Msa0320990-mRNA-1
chr2_4	exon	21993859	21993923	21993859	ID=Msa0320990-mRNA-1:exon:8097;Parent=Msa0320990-mRNA-1
chr2_4	exon	21994006	21994097	21994006	ID=Msa0320990-mRNA-1:exon:8096;Parent=Msa0320990-mRNA-1
chr2_4	exon	21994294	21994470	21994294	ID=Msa0320990-mRNA-1:exon:8095;Parent=Msa0320990-mRNA-1
chr2_4	exon	21996326	21996475	21996326	ID=Msa0320990-mRNA-1:exon:8094;Parent=Msa0320990-mRNA-1
chr2_4	exon	21996575	21996838	21996575	ID=Msa0320990-mRNA-1:exon:8093;Parent=Msa0320990-mRNA-1
chr2_4	exon	22001257	22001340	22001257	ID=Msa0320990-mRNA-1:exon:8092;Parent=Msa0320990-mRNA-1
chr2_4	exon	22002070	22002172	22002070	ID=Msa0320990-mRNA-1:exon:8091;Parent=Msa0320990-mRNA-1
chr2_4	exon	22002279	22002343	22002279	ID=Msa0320990-mRNA-1:exon:8090;Parent=Msa0320990-mRNA-1
chr2_4	exon	22002431	22002522	22002431	ID=Msa0320990-mRNA-1:exon:8089;Parent=Msa0320990-mRNA-1
chr2_4	exon	22002765	22002941	22002765	ID=Msa0320990-mRNA-1:exon:8088;Parent=Msa0320990-mRNA-1
chr2_4	exon	22004813	22004962	22004813	ID=Msa0320990-mRNA-1:exon:8087;Parent=Msa0320990-mRNA-1
chr2_4	exon	22005074	22005236	22005074	ID=Msa0320990-mRNA-1:exon:8086;Parent=Msa0320990-mRNA-1
chr2_4	exon	22010486	22010512	22010486	ID=Msa0320990-mRNA-1:exon:8085;Parent=Msa0320990-mRNA-1
chr2_4	CDS	22010486	22010512	22010486	ID=Msa0320990-mRNA-1:cds;Parent=Msa0320990-mRNA-1
chr2_4	CDS	22005074	22005236	22005074	ID=Msa0320990-mRNA-1:cds;Parent=Msa0320990-mRNA-1
chr2_4	CDS	22004813	22004962	22004813	ID=Msa0320990-mRNA-1:cds;Parent=Msa0320990-mRNA-1
chr2_4	CDS	22002765	22002941	22002765	ID=Msa0320990-mRNA-1:cds;Parent=Msa0320990-mRNA-1
chr2_4	CDS	22002431	22002522	22002431	ID=Msa0320990-mRNA-1:cds;Parent=Msa0320990-mRNA-1
chr2_4	CDS	22002279	22002343	22002279	ID=Msa0320990-mRNA-1:cds;Parent=Msa0320990-mRNA-1
chr2_4	CDS	22002070	22002172	22002070	ID=Msa0320990-mRNA-1:cds;Parent=Msa0320990-mRNA-1
chr2_4	CDS	22001257	22001340	22001257	ID=Msa0320990-mRNA-1:cds;Parent=Msa0320990-mRNA-1
chr2_4	CDS	21996803	21996838	21996803	ID=Msa0320990-mRNA-1:cds;Parent=Msa0320990-mRNA-1
chr2_4	three_prime_UTR	21996575	21996802	21996575	ID=Msa0320990-mRNA-1:three_prime_utr;Parent=Msa0320990-mRNA-1
chr2_4	three_prime_UTR	21996326	21996475	21996326	ID=Msa0320990-mRNA-1:three_prime_utr;Parent=Msa0320990-mRNA-1
chr2_4	three_prime_UTR	21994294	21994470	21994294	ID=Msa0320990-mRNA-1:three_prime_utr;Parent=Msa0320990-mRNA-1
chr2_4	three_prime_UTR	21994006	21994097	21994006	ID=Msa0320990-mRNA-1:three_prime_utr;Parent=Msa0320990-mRNA-1
chr2_4	three_prime_UTR	21993859	21993923	21993859	ID=Msa0320990-mRNA-1:three_prime_utr;Parent=Msa0320990-mRNA-1
chr2_4	three_prime_UTR	21993662	21993764	21993662	ID=Msa0320990-mRNA-1:three_prime_utr;Parent=Msa0320990-mRNA-1
chr2_4	three_prime_UTR	21993362	21993472	21993362	ID=Msa0320990-mRNA-1:three_prime_utr;Parent=Msa0320990-mRNA-1
chr2_4	three_prime_UTR	21992782	21993275	21992782	ID=Msa0320990-mRNA-1:three_prime_utr;Parent=Msa0320990-mRNA-1

Gene Sequence

>Msa0320990

ATGGTTGCTGAGCTTTTCTTTGAGGAGTTAAACTTAAGCAATTCATTACATTTGCGTTGCTTCATTTTTCTAACAATGGGAGCTGCACCATCAACTTCCATAGTTGAAGCTAAAATTTCAGCCATTTTCATTTATCCAATTAAGTCATGCCGTGGAATTTCTCTTTCTCATGCTCCTCTCACTCCTTCTGGACTTCGATGGGATCGCCAGTGGGTGGTTGTGAATTCAAAAGGAAGAGCATGCACTCAAAGAGTTGAACCAAAACTTGCTTTGGTTGAGGTTGAACTTCCACCTGAGGCTTTTGATGAACACTGGGAACCTACCACTGATTCCTTTATGGTGTTGAAAGCACCTGGAATGGAACCTCTCAAAGTTTGCTTGAACAAACAGTATGAAGTAGTAGATGATATTACAGTGTGGGAATGGACTGGCTCTGCTTGGGACGAGGGAGCCGAAGCATCACAGTGGTTCTCAGACTATTTAGGAAATCCTACTAAACTGGTTCGCTTCAATACTGCTTCAGAAGTTAGAAAAGTAGATCCTGACTATGTTGAGGGACAGCACCAGACCTTTTTCAGCGATGGTTATCCATTCTTACTCGTATCTCAGGAATCATTGGATGCACTAAACGAGCATCTAAAGGAACCGATACCTATGAATCGTTTCAGACCCAATATCCTTGTTGAAGGTTGTGAACCATATTCTGAAGACCTATGGAGAGATATCAAGATAAGCAGGTTTTCATTTCAGGGTGTCAAGCTGTGCGACCGCTGCAAGGTCTACTTTGGTCAGAATTTAGTGTGGAACTGGAAGGATTCTTCTGCTAAAGGGGATGGAAAAGTCCTTAAATTGGGAGATCCTTGTTACGTGGCCCCTCATGTGAAATGTGATGTTTAA

Protein sequence

>Msa0320990

MVAELFFEELNLSNSLHLRCFIFLTMGAAPSTSIVEAKISAIFIYPIKSCRGISLSHAPLTPSGLRWDRQWVVVNSKGRACTQRVEPKLALVEVELPPEAFDEHWEPTTDSFMVLKAPGMEPLKVCLNKQYEVVDDITVWEWTGSAWDEGAEASQWFSDYLGNPTKLVRFNTASEVRKVDPDYVEGQHQTFFSDGYPFLLVSQESLDALNEHLKEPIPMNRFRPNILVEGCEPYSEDLWRDIKISRFSFQGVKLCDRCKVYFGQNLVWNWKDSSAKGDGKVLKLGDPCYVAPHVKCDV*