AlfalfaGEDB | Msa0321530

M. sativa cultivar zhongmu-4 / Msa0321530

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321530	XP_003595002.1	53.333	75	33	1	155	229	19	91	8.15e-15	79.7
Msa0321530	XP_003595002.1	83.333	126	17	1	1	122	5	130	8.54e-68	215

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321530	sp\|P11143\|HSP70_MAIZE	87.097	31	4	0	123	153	5	35	3.10e-11	65.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0321530	Msa0947420	0.808238	3.416674e-50	-8.615850e-47
Msa0321530	Msa1062060	0.805462	1.319538e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321530	MtrunA17_Chr2g0298321	83.333	126	17	1	1	122	5	130	7.85e-72	215
Msa0321530	MtrunA17_Chr2g0298321	53.333	75	33	1	155	229	19	91	7.50e-19	79.7
Msa0321530	MtrunA17_Chr2g0298331	69.291	127	34	2	1	122	5	131	5.61e-57	177
Msa0321530	MtrunA17_Chr2g0298331	53.947	76	32	2	155	229	19	92	2.66e-18	78.2
Msa0321530	MtrunA17_Chr2g0298361	90.411	73	7	0	157	229	21	93	2.06e-42	140
Msa0321530	MtrunA17_Chr2g0298361	47.321	112	53	2	18	123	22	133	1.36e-26	99.8
Msa0321530	MtrunA17_Chr2g0298341	81.579	76	14	0	155	230	15	90	1.42e-40	135
Msa0321530	MtrunA17_Chr2g0298341	46.875	128	62	2	1	122	1	128	6.03e-35	121
Msa0321530	MtrunA17_Chr2g0298351	49.612	129	59	2	1	123	1	129	1.71e-38	130
Msa0321530	MtrunA17_Chr2g0298351	64.474	76	27	0	155	230	15	90	6.88e-29	105

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321530	AT5G02490.1	87.097	31	4	123	153	6	36	3.54e-12	65.9
Msa0321530	AT3G09440.1	87.097	31	4	123	153	6	36	3.81e-12	65.9
Msa0321530	AT3G09440.4	87.097	31	4	123	153	6	36	3.81e-12	65.9
Msa0321530	AT3G09440.2	87.097	31	4	123	153	6	36	3.81e-12	65.9
Msa0321530	AT3G09440.3	87.097	31	4	123	153	6	36	3.81e-12	65.9
Msa0321530	AT5G02500.1	87.097	31	4	123	153	6	36	3.81e-12	65.9
Msa0321530	AT3G12580.1	87.097	31	4	123	153	6	36	3.92e-12	65.9
Msa0321530	AT1G56410.1	87.097	31	4	123	153	6	36	3.93e-12	65.9
Msa0321530	AT5G02500.2	87.097	31	4	123	153	6	36	4.02e-12	65.5

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Find 46 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTGAAGGATCTGCAAT+TGG	0.465546	2_4:+22890896	Msa0321530:intron
AACACGAACGCCTCTTGCAA+AGG	0.569144	2_4:-22889605	None:intergenic
AACCAAATGTCAAAATCTTT+CGG	0.331219	2_4:+22889017	None:intergenic
AAGATGATTGTTGCAACAAA+AGG	0.512720	2_4:+22889746	Msa0321530:CDS
AAGGCAAAGAATCATATCAA+AGG	0.584033	2_4:-22889815	None:intergenic
AAGTCACAACTCGATCCTAT+AGG	0.502354	2_4:-22892609	None:intergenic
AATCAATGCGATATCGGGAT+GGG	0.569192	2_4:+22892687	Msa0321530:CDS
ACTCCAAATTGTCCTGTTAC+TGG	0.264283	2_4:-22892663	None:intergenic
AGATATAGTAAAACGGGTGC+TGG	0.414910	2_4:+22889778	Msa0321530:intron
AGATCAAACTCAACGATTAT+AGG	0.281966	2_4:-22889842	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCAACAACTAAAATCAAGA+TGG	-	chr2_4:22889410-22889429	None:intergenic	30.0%
CACCTGGTAATTGTATTATT+GGG	+	chr2_4:22889698-22889717	Msa0321530:CDS	30.0%
ATCCCAATAATACAATTACC+AGG	-	chr2_4:22889703-22889722	None:intergenic	30.0%
TGGTAATTGTATTATTGGGA+TGG	+	chr2_4:22889702-22889721	Msa0321530:CDS	30.0%
GGTAATTGTATTATTGGGAT+GGG	+	chr2_4:22889703-22889722	Msa0321530:CDS	30.0%
AAGATGATTGTTGCAACAAA+AGG	+	chr2_4:22889746-22889765	Msa0321530:CDS	30.0%
AAGGCAAAGAATCATATCAA+AGG	-	chr2_4:22889818-22889837	None:intergenic	30.0%
AGATCAAACTCAACGATTAT+AGG	-	chr2_4:22889845-22889864	None:intergenic	30.0%
ATCGTTGAGTTTGATCTTAA+AGG	+	chr2_4:22889848-22889867	Msa0321530:intron	30.0%
TGGAATTAAAGGTCATGATT+TGG	-	chr2_4:22890511-22890530	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	22889023	22893576	22889023	ID=Msa0321530;Name=Msa0321530
chr2_4	mRNA	22889023	22893576	22889023	ID=Msa0321530-mRNA-1;Parent=Msa0321530;Name=Msa0321530-mRNA-1;_AED=0.20;_eAED=0.21;_QI=0\|0\|0\|0.85\|0.16\|0.14\|7\|0\|234
chr2_4	exon	22889023	22889071	22889023	ID=Msa0321530-mRNA-1:exon:8421;Parent=Msa0321530-mRNA-1
chr2_4	exon	22889524	22889767	22889524	ID=Msa0321530-mRNA-1:exon:8422;Parent=Msa0321530-mRNA-1
chr2_4	exon	22889780	22889850	22889780	ID=Msa0321530-mRNA-1:exon:8423;Parent=Msa0321530-mRNA-1
chr2_4	exon	22890900	22890995	22890900	ID=Msa0321530-mRNA-1:exon:8424;Parent=Msa0321530-mRNA-1
chr2_4	exon	22892401	22892409	22892401	ID=Msa0321530-mRNA-1:exon:8425;Parent=Msa0321530-mRNA-1
chr2_4	exon	22892502	22892720	22892502	ID=Msa0321530-mRNA-1:exon:8426;Parent=Msa0321530-mRNA-1
chr2_4	exon	22893560	22893576	22893560	ID=Msa0321530-mRNA-1:exon:8427;Parent=Msa0321530-mRNA-1
chr2_4	CDS	22889023	22889071	22889023	ID=Msa0321530-mRNA-1:cds;Parent=Msa0321530-mRNA-1
chr2_4	CDS	22889524	22889767	22889524	ID=Msa0321530-mRNA-1:cds;Parent=Msa0321530-mRNA-1
chr2_4	CDS	22889780	22889850	22889780	ID=Msa0321530-mRNA-1:cds;Parent=Msa0321530-mRNA-1
chr2_4	CDS	22890900	22890995	22890900	ID=Msa0321530-mRNA-1:cds;Parent=Msa0321530-mRNA-1
chr2_4	CDS	22892401	22892409	22892401	ID=Msa0321530-mRNA-1:cds;Parent=Msa0321530-mRNA-1
chr2_4	CDS	22892502	22892720	22892502	ID=Msa0321530-mRNA-1:cds;Parent=Msa0321530-mRNA-1
chr2_4	CDS	22893560	22893576	22893560	ID=Msa0321530-mRNA-1:cds;Parent=Msa0321530-mRNA-1

Gene Sequence

>Msa0321530

ATGTCAAAATCTTTCGGTTTCTTTGCATTTTTGGCTTTAGTTGTTGCAGGGAAATTAATACAGGTAGAAGGTGAATGCACAAAAATTGTTGGTAGTTGTGCAAAAGAAGATTGTTCTGAACACTGTAATTCCTTTGCAAGAGGCGTTCGTGTTTTAGGATCATCATGTTCATACTACAACTTATGCACTTGTACTTTTGATCAGCCACCACCAGGATTAACACCACCTGGTAATTGTATTATTGGGATGGGAATTTGCACTCGTGACTGTCAAGATGATTGTTGCAACAAAAGATATAGTAAAACGGGTGCTGGGAACTGTGTTGATGCCTTTGATATGATTCTTTGCCTTTGTTCCTATAATCGTGAAGGATCTGCAATTGGAATGGATTTGGGAACAACTTATTCATGTATTGGTGCTTGGCAACATGATCGTGTTGAGATCATAGCAAATGATCAATTTGTTGCAGTGCAATTAATAGAGGTTGAAGGTGTTTGCACAAACATTGTGGCTAATTGTGCTGATGAACATGTACATTGTCCATCGCAATGTGAAGCCTTTGGAAGAGGCGCTAAACCTATAGGATCGAGTTGTGACTTCTACAACTTATGCACTTGTTCTTACGAACATCCAGTAACAGGACAATTTGGAGTTAATCAATGCGATATCGGGATGGGACTATGCACTAGCTTGGCTTTTGCCTAG

Protein sequence

>Msa0321530

MSKSFGFFAFLALVVAGKLIQVEGECTKIVGSCAKEDCSEHCNSFARGVRVLGSSCSYYNLCTCTFDQPPPGLTPPGNCIIGMGICTRDCQDDCCNKRYSKTGAGNCVDAFDMILCLCSYNREGSAIGMDLGTTYSCIGAWQHDRVEIIANDQFVAVQLIEVEGVCTNIVANCADEHVHCPSQCEAFGRGAKPIGSSCDFYNLCTCSYEHPVTGQFGVNQCDIGMGLCTSLAFA*