AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0326460


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGATCTTAAATCCTTTA+AGG 0.154933 2_4:+36349970 Msa0326460:CDS
GAAATTATATGTTACCTTAA+AGG 0.158934 2_4:-36349984 None:intergenic
TCCTTCCTGATTATTGCTTT+TGG 0.239235 2_4:-36352043 None:intergenic
AGAAGAAGAAGTAATCAATT+TGG 0.277514 2_4:+36349919 Msa0326460:five_prime_UTR
AGTGGTGAGAAAAGGGAAAA+TGG 0.278092 2_4:-36349723 None:intergenic
AAAGCTCCTACACCAACATT+AGG 0.347170 2_4:+36349864 Msa0326460:five_prime_UTR
AAGGATTTAAGATCCATCTT+TGG 0.355406 2_4:-36349965 None:intergenic
GAAGAAGAAGTAATCAATTT+GGG 0.363060 2_4:+36349920 Msa0326460:five_prime_UTR
AGTAATCAATTTGGGTATGT+TGG 0.388725 2_4:+36349928 Msa0326460:exon
TGTCGAGAGAGAAGATGAAA+GGG 0.388822 2_4:+36349767 Msa0326460:five_prime_UTR
ATGGACCAAAAGCAATAATC+AGG 0.422893 2_4:+36352038 Msa0326460:CDS
TTATGAAGTGGGAAGGCTTA+AGG 0.429941 2_4:-36349829 None:intergenic
TCTACTGTAGTGGTGAGAAA+AGG 0.444555 2_4:-36349731 None:intergenic
CAATAATCAGGAAGGAAGTC+TGG 0.445314 2_4:+36352050 Msa0326460:CDS
AAGCTCCTACACCAACATTA+GGG 0.460989 2_4:+36349865 Msa0326460:five_prime_UTR
GTGGTGAGAAAAGGGAAAAT+GGG 0.461366 2_4:-36349722 None:intergenic
CTACTGTAGTGGTGAGAAAA+GGG 0.466765 2_4:-36349730 None:intergenic
TTTCTGTCTAACCCTAATGT+TGG 0.471425 2_4:-36349876 None:intergenic
TTGTCGAGAGAGAAGATGAA+AGG 0.497904 2_4:+36349766 Msa0326460:five_prime_UTR
ACAAACAATTGAAGATATGA+AGG 0.499490 2_4:+36352083 Msa0326460:CDS
AATCAATTTGGGTATGTTGG+AGG 0.501098 2_4:+36349931 Msa0326460:exon
CTATGGGGAAATTATGAAGT+GGG 0.502485 2_4:-36349840 None:intergenic
GGTTGAAGAAGAACCAAAGA+TGG 0.535814 2_4:+36349952 Msa0326460:CDS
AGGGAAAATGGGTATAGAGT+TGG 0.546792 2_4:-36349711 None:intergenic
GTCGAGAGAGAAGATGAAAG+GGG 0.569334 2_4:+36349768 Msa0326460:five_prime_UTR
TCTATGGGGAAATTATGAAG+TGG 0.604070 2_4:-36349841 None:intergenic
GGGGAAATTATGAAGTGGGA+AGG 0.605746 2_4:-36349836 None:intergenic
TCTAACCCTAATGTTGGTGT+AGG 0.608709 2_4:-36349870 None:intergenic
GCTATGCAATGAAATGATAG+AGG 0.613912 2_4:-36349799 None:intergenic
ACCAAAAGCAATAATCAGGA+AGG 0.620971 2_4:+36352042 Msa0326460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATATAATTGAGATATTT+AGG + chr2_4:36350051-36350070 Msa0326460:intron 10.0%
!! TAATATAATTGAGATATTTA+GGG + chr2_4:36350052-36350071 Msa0326460:intron 10.0%
!!! AATGAAATCAAATAATTTTG+TGG - chr2_4:36350588-36350607 None:intergenic 15.0%
!! TTAAATGATGATTTAATAGA+TGG + chr2_4:36350746-36350765 Msa0326460:intron 15.0%
!! CAAAAAAAAAAAAAAAGGAA+TGG - chr2_4:36350803-36350822 None:intergenic 15.0%
!!! TTCCTTTTTTTTTTTTTTTG+AGG + chr2_4:36350803-36350822 Msa0326460:intron 15.0%
!!! TCCTTTTTTTTTTTTTTTGA+GGG + chr2_4:36350804-36350823 Msa0326460:intron 15.0%
!!! TTTTTAATGAAGTCTTTTAA+AGG + chr2_4:36351651-36351670 Msa0326460:intron 15.0%
!!! TTTTAATGAAGTCTTTTAAA+GGG + chr2_4:36351652-36351671 Msa0326460:intron 15.0%
!! AATAAAGTTTAAACTGAAAT+TGG + chr2_4:36351727-36351746 Msa0326460:intron 15.0%
!! ATAAAGTTTAAACTGAAATT+GGG + chr2_4:36351728-36351747 Msa0326460:intron 15.0%
!! GAAATTATATGTTACCTTAA+AGG - chr2_4:36349987-36350006 None:intergenic 20.0%
!!! ATTTTGCATTTGATTTTGAT+AGG + chr2_4:36350132-36350151 Msa0326460:intron 20.0%
!!! ACTGATTTGAACTTTAATTT+TGG + chr2_4:36350188-36350207 Msa0326460:intron 20.0%
!! TTTAGATATAGATAGAAAGA+AGG + chr2_4:36350235-36350254 Msa0326460:intron 20.0%
!! AAATTCAAGGTAATTAATTC+AGG - chr2_4:36350509-36350528 None:intergenic 20.0%
!!! AATTACCTTGAATTTTGATA+TGG + chr2_4:36350514-36350533 Msa0326460:intron 20.0%
!!! ATTACCTTGAATTTTGATAT+GGG + chr2_4:36350515-36350534 Msa0326460:intron 20.0%
!!! TAATTTTGTGGATTTTACTT+GGG - chr2_4:36350576-36350595 None:intergenic 20.0%
!!! ATAATTTTGTGGATTTTACT+TGG - chr2_4:36350577-36350596 None:intergenic 20.0%
!!! TATTTCTAAACTATTCAAAG+TGG + chr2_4:36350667-36350686 Msa0326460:intron 20.0%
!! TCCCTCAAAAAAAAAAAAAA+AGG - chr2_4:36350808-36350827 None:intergenic 20.0%
!! TCCTTATTAAAGAGTTATAA+TGG + chr2_4:36350896-36350915 Msa0326460:intron 20.0%
!!! CATTCTTAGTTTCAAAATTT+TGG - chr2_4:36351993-36352012 None:intergenic 20.0%
! AGAAGAAGAAGTAATCAATT+TGG + chr2_4:36349919-36349938 Msa0326460:five_prime_UTR 25.0%
! GAAGAAGAAGTAATCAATTT+GGG + chr2_4:36349920-36349939 Msa0326460:five_prime_UTR 25.0%
!!! AGTTGATTTGATTGATTGTT+TGG + chr2_4:36350087-36350106 Msa0326460:intron 25.0%
! ATTTGATTGATTGTTTGGTT+TGG + chr2_4:36350092-36350111 Msa0326460:intron 25.0%
!! GGTGACTTTTAATAACTTAT+TGG + chr2_4:36350314-36350333 Msa0326460:intron 25.0%
! CACGACAAAAATAACTAAAA+CGG - chr2_4:36350776-36350795 None:intergenic 25.0%
! GCCATTATAACTCTTTAATA+AGG - chr2_4:36350900-36350919 None:intergenic 25.0%
!!! CATAATAGTTTTGTGATGTA+TGG + chr2_4:36350919-36350938 Msa0326460:intron 25.0%
!!! GTTTTTGAGATTTAATGAGA+AGG + chr2_4:36351391-36351410 Msa0326460:intron 25.0%
!! AGTTTTACTCACTATGTATT+TGG + chr2_4:36351521-36351540 Msa0326460:intron 25.0%
!! GTTTTACTCACTATGTATTT+GGG + chr2_4:36351522-36351541 Msa0326460:intron 25.0%
!! TTTTACTCACTATGTATTTG+GGG + chr2_4:36351523-36351542 Msa0326460:intron 25.0%
AGTAATCAATTTGGGTATGT+TGG + chr2_4:36349928-36349947 Msa0326460:exon 30.0%
! AAGGATTTAAGATCCATCTT+TGG - chr2_4:36349968-36349987 None:intergenic 30.0%
GATGGATCTTAAATCCTTTA+AGG + chr2_4:36349970-36349989 Msa0326460:CDS 30.0%
! ATGTTTGTGCAGAAATTTTC+AGG - chr2_4:36350286-36350305 None:intergenic 30.0%
ATTGGCATGTGATATTCATA+TGG + chr2_4:36350332-36350351 Msa0326460:intron 30.0%
TTGGCATGTGATATTCATAT+GGG + chr2_4:36350333-36350352 Msa0326460:intron 30.0%
!! TGCATTAGTCTTATGTTCAT+TGG + chr2_4:36350465-36350484 Msa0326460:intron 30.0%
TGTTCCCATATCAAAATTCA+AGG - chr2_4:36350522-36350541 None:intergenic 30.0%
! AATTGTCTTTGTTTGTGAAC+TGG + chr2_4:36350546-36350565 Msa0326460:intron 30.0%
CTTTCTATAATCATTGCAAG+AGG + chr2_4:36350702-36350721 Msa0326460:intron 30.0%
!!! ATAGTTTTGTGATGTATGGT+TGG + chr2_4:36350923-36350942 Msa0326460:intron 30.0%
! GATGTATGGTTGGTTATTAA+AGG + chr2_4:36350933-36350952 Msa0326460:intron 30.0%
TGGTTATTAAAGGTTGAAGA+AGG + chr2_4:36350943-36350962 Msa0326460:intron 30.0%
! ACTTTTCTCATGTCATTGAA+CGG - chr2_4:36351004-36351023 None:intergenic 30.0%
TATTAAGATTTACCCTTGCA+AGG + chr2_4:36351164-36351183 Msa0326460:intron 30.0%
!!! TTCATAGTTTTAACCTCTTC+TGG - chr2_4:36351266-36351285 None:intergenic 30.0%
TCATTAAAAACTCTAGACTC+TGG - chr2_4:36351641-36351660 None:intergenic 30.0%
CTTCTCTTACTAAAAGAAGT+GGG + chr2_4:36351823-36351842 Msa0326460:intron 30.0%
TTCTCTTACTAAAAGAAGTG+GGG + chr2_4:36351824-36351843 Msa0326460:intron 30.0%
TGCCAATTCATAATTCAGAA+TGG + chr2_4:36352019-36352038 Msa0326460:intron 30.0%
GTCCATTCTGAATTATGAAT+TGG - chr2_4:36352024-36352043 None:intergenic 30.0%
GCTATGCAATGAAATGATAG+AGG - chr2_4:36349802-36349821 None:intergenic 35.0%
CTATGGGGAAATTATGAAGT+GGG - chr2_4:36349843-36349862 None:intergenic 35.0%
TCTATGGGGAAATTATGAAG+TGG - chr2_4:36349844-36349863 None:intergenic 35.0%
TTTCTGTCTAACCCTAATGT+TGG - chr2_4:36349879-36349898 None:intergenic 35.0%
AATCAATTTGGGTATGTTGG+AGG + chr2_4:36349931-36349950 Msa0326460:exon 35.0%
TTCTCTTCTTAAACCTCTTC+AGG + chr2_4:36350004-36350023 Msa0326460:intron 35.0%
TCTCTTCTTAAACCTCTTCA+GGG + chr2_4:36350005-36350024 Msa0326460:intron 35.0%
AGGGTGTGCTTATAAATTGT+TGG + chr2_4:36350024-36350043 Msa0326460:intron 35.0%
GAAGATAGTCAACTTGCATA+TGG - chr2_4:36350375-36350394 None:intergenic 35.0%
CTATTCAAAGTGGTGAATCA+TGG + chr2_4:36350677-36350696 Msa0326460:intron 35.0%
TAACCATTCCTAAAGAACAG+AGG + chr2_4:36350834-36350853 Msa0326460:intron 35.0%
! AAACCTCTGTTCTTTAGGAA+TGG - chr2_4:36350840-36350859 None:intergenic 35.0%
TTCTCAAACCTCTGTTCTTT+AGG - chr2_4:36350845-36350864 None:intergenic 35.0%
AACACGTTTAATAGCGTGAA+TGG + chr2_4:36351042-36351061 Msa0326460:intron 35.0%
TTAACCTCTTCTGGTAAGAT+TGG - chr2_4:36351257-36351276 None:intergenic 35.0%
TAAAACGTTTCCAACCATGT+CGG - chr2_4:36351471-36351490 None:intergenic 35.0%
GCTTCTCTTACTAAAAGAAG+TGG + chr2_4:36351822-36351841 Msa0326460:intron 35.0%
!!! ACATTTTTGAAACCCTCACT+CGG - chr2_4:36351872-36351891 None:intergenic 35.0%
AACAATTAAGCCATCTGATG+TGG + chr2_4:36351898-36351917 Msa0326460:intron 35.0%
GCTAATGTGGATAAGGAATT+AGG + chr2_4:36351966-36351985 Msa0326460:intron 35.0%
ATGGACCAAAAGCAATAATC+AGG + chr2_4:36352038-36352057 Msa0326460:CDS 35.0%
ACCAAAAGCAATAATCAGGA+AGG + chr2_4:36352042-36352061 Msa0326460:CDS 35.0%
! TCCTTCCTGATTATTGCTTT+TGG - chr2_4:36352046-36352065 None:intergenic 35.0%
GTGGTGAGAAAAGGGAAAAT+GGG - chr2_4:36349725-36349744 None:intergenic 40.0%
AGTGGTGAGAAAAGGGAAAA+TGG - chr2_4:36349726-36349745 None:intergenic 40.0%
CTACTGTAGTGGTGAGAAAA+GGG - chr2_4:36349733-36349752 None:intergenic 40.0%
TCTACTGTAGTGGTGAGAAA+AGG - chr2_4:36349734-36349753 None:intergenic 40.0%
TTGTCGAGAGAGAAGATGAA+AGG + chr2_4:36349766-36349785 Msa0326460:five_prime_UTR 40.0%
TGTCGAGAGAGAAGATGAAA+GGG + chr2_4:36349767-36349786 Msa0326460:five_prime_UTR 40.0%
TTATGAAGTGGGAAGGCTTA+AGG - chr2_4:36349832-36349851 None:intergenic 40.0%
! TGGTGTAGGAGCTTTTCTAT+GGG - chr2_4:36349859-36349878 None:intergenic 40.0%
! TTGGTGTAGGAGCTTTTCTA+TGG - chr2_4:36349860-36349879 None:intergenic 40.0%
AAAGCTCCTACACCAACATT+AGG + chr2_4:36349864-36349883 Msa0326460:five_prime_UTR 40.0%
AAGCTCCTACACCAACATTA+GGG + chr2_4:36349865-36349884 Msa0326460:five_prime_UTR 40.0%
TCTAACCCTAATGTTGGTGT+AGG - chr2_4:36349873-36349892 None:intergenic 40.0%
GGTTGAAGAAGAACCAAAGA+TGG + chr2_4:36349952-36349971 Msa0326460:CDS 40.0%
TTTATAAGCACACCCTGAAG+AGG - chr2_4:36350020-36350039 None:intergenic 40.0%
TCTGCACAAACATGTTCAGT+TGG + chr2_4:36350293-36350312 Msa0326460:intron 40.0%
AGTGCTCCGTCTATATATGA+CGG + chr2_4:36351189-36351208 Msa0326460:intron 40.0%
CATGTCCCGTCATATATAGA+CGG - chr2_4:36351198-36351217 None:intergenic 40.0%
ATCTCCAATCTTACCAGAAG+AGG + chr2_4:36351250-36351269 Msa0326460:intron 40.0%
CACACTTAGTGACTTAGACA+AGG + chr2_4:36351335-36351354 Msa0326460:intron 40.0%
ACTTAGTGACTTAGACAAGG+AGG + chr2_4:36351338-36351357 Msa0326460:intron 40.0%
CTAATGTGCACTTCTATACC+TGG - chr2_4:36351557-36351576 None:intergenic 40.0%
TGTGCATGCAAATGATCTGA+TGG + chr2_4:36351768-36351787 Msa0326460:intron 40.0%
! GCAAATGATCTGATGGATGA+TGG + chr2_4:36351775-36351794 Msa0326460:intron 40.0%
AATTAAGCCATCTGATGTGG+AGG + chr2_4:36351901-36351920 Msa0326460:intron 40.0%
ATTAAGCCATCTGATGTGGA+GGG + chr2_4:36351902-36351921 Msa0326460:intron 40.0%
CAATAATCAGGAAGGAAGTC+TGG + chr2_4:36352050-36352069 Msa0326460:CDS 40.0%
TCTCTCTCTCTCTACTGTAG+TGG - chr2_4:36349744-36349763 None:intergenic 45.0%
GTCGAGAGAGAAGATGAAAG+GGG + chr2_4:36349768-36349787 Msa0326460:five_prime_UTR 45.0%
GGGGAAATTATGAAGTGGGA+AGG - chr2_4:36349839-36349858 None:intergenic 45.0%
! GGTGTAGGAGCTTTTCTATG+GGG - chr2_4:36349858-36349877 None:intergenic 45.0%
GCATATGGACACGATGCAAT+TGG - chr2_4:36350360-36350379 None:intergenic 45.0%
GTGCTCCGTCTATATATGAC+GGG + chr2_4:36351190-36351209 Msa0326460:intron 45.0%
ACATGTAAGCGAGGGTAAAC+AGG + chr2_4:36351213-36351232 Msa0326460:intron 45.0%
CATGTAAGCGAGGGTAAACA+GGG + chr2_4:36351214-36351233 Msa0326460:intron 45.0%
! GTATTTGGGGTTACGTTTCC+AGG + chr2_4:36351536-36351555 Msa0326460:intron 45.0%
TCAGCCGTAAATCGTTCAGT+CGG + chr2_4:36351602-36351621 Msa0326460:intron 45.0%
TCTAGACTCTGGACGTGATT+CGG - chr2_4:36351630-36351649 None:intergenic 45.0%
CACTCGGAGCAAGATTCTAA+TGG - chr2_4:36351856-36351875 None:intergenic 45.0%
TTAGAATCTTGCTCCGAGTG+AGG + chr2_4:36351856-36351875 Msa0326460:intron 45.0%
TAGAATCTTGCTCCGAGTGA+GGG + chr2_4:36351857-36351876 Msa0326460:intron 45.0%
! TATGACGGGACATGTAAGCG+AGG + chr2_4:36351204-36351223 Msa0326460:intron 50.0%
! ATGACGGGACATGTAAGCGA+GGG + chr2_4:36351205-36351224 Msa0326460:intron 50.0%
CCATGTCGGTGTGCTAAACA+TGG - chr2_4:36351457-36351476 None:intergenic 50.0%
CCATGTTTAGCACACCGACA+TGG + chr2_4:36351454-36351473 Msa0326460:intron 50.0%
GTTTAGCACACCGACATGGT+TGG + chr2_4:36351458-36351477 Msa0326460:intron 50.0%
GGCTCCGACTGAACGATTTA+CGG - chr2_4:36351609-36351628 None:intergenic 50.0%
CTTTCTCCCTCCACATCAGA+TGG - chr2_4:36351911-36351930 None:intergenic 50.0%
! ACATTAGCCTCCCCAAACTC+GGG - chr2_4:36351954-36351973 None:intergenic 50.0%
! CACATTAGCCTCCCCAAACT+CGG - chr2_4:36351955-36351974 None:intergenic 50.0%
TGGGGAGGCTAATGTGGATA+AGG + chr2_4:36351959-36351978 Msa0326460:intron 50.0%
! ACGGAGCACTCACCTTGCAA+GGG - chr2_4:36351179-36351198 None:intergenic 55.0%
TTAGTCCCGACTCCCGAGTT+TGG + chr2_4:36351939-36351958 Msa0326460:intron 55.0%
TAGTCCCGACTCCCGAGTTT+GGG + chr2_4:36351940-36351959 Msa0326460:intron 55.0%
CGAGTTTGGGGAGGCTAATG+TGG + chr2_4:36351953-36351972 Msa0326460:intron 55.0%
! GACGGAGCACTCACCTTGCA+AGG - chr2_4:36351180-36351199 None:intergenic 60.0%
CGGGAGTCGGGACTAACTAC+TGG - chr2_4:36351935-36351954 None:intergenic 60.0%
AGTCCCGACTCCCGAGTTTG+GGG + chr2_4:36351941-36351960 Msa0326460:intron 60.0%
CCTCCCCAAACTCGGGAGTC+GGG - chr2_4:36351947-36351966 None:intergenic 65.0%
GCCTCCCCAAACTCGGGAGT+CGG - chr2_4:36351948-36351967 None:intergenic 65.0%
CCCGACTCCCGAGTTTGGGG+AGG + chr2_4:36351944-36351963 Msa0326460:intron 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 36349712 36352102 36349712 ID=Msa0326460;Name=Msa0326460
chr2_4 mRNA 36349712 36352102 36349712 ID=Msa0326460-mRNA-1;Parent=Msa0326460;Name=Msa0326460-mRNA-1;_AED=0.38;_eAED=0.50;_QI=232|0|0.5|1|0|0.5|2|0|37
chr2_4 exon 36349712 36349991 36349712 ID=Msa0326460-mRNA-1:exon:11177;Parent=Msa0326460-mRNA-1
chr2_4 exon 36352037 36352102 36352037 ID=Msa0326460-mRNA-1:exon:11178;Parent=Msa0326460-mRNA-1
chr2_4 five_prime_UTR 36349712 36349943 36349712 ID=Msa0326460-mRNA-1:five_prime_utr;Parent=Msa0326460-mRNA-1
chr2_4 CDS 36349944 36349991 36349944 ID=Msa0326460-mRNA-1:cds;Parent=Msa0326460-mRNA-1
chr2_4 CDS 36352037 36352102 36352037 ID=Msa0326460-mRNA-1:cds;Parent=Msa0326460-mRNA-1
Gene Sequence

>Msa0326460

ATGTTGGAGGTTGAAGAAGAACCAAAGATGGATCTTAAATCCTTTAAGAATGGACCAAAAGCAATAATCAGGAAGGAAGTCTGGCAGCGTAGAAACAAACAATTGAAGATATGA

Protein sequence

>Msa0326460

MLEVEEEPKMDLKSFKNGPKAIIRKEVWQRRNKQLKI*