AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1430410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGATCTTAAATCCTTTA+AGG 0.154933 tig0026649:+22983 Msa1430410:CDS
GAAATTATATGTTACCTTAA+AGG 0.158934 tig0026649:-22997 None:intergenic
TCCTTCCTGATTATTGCTTT+TGG 0.239235 tig0026649:-25049 None:intergenic
AGAAGAAGAAGTAATCAATT+TGG 0.277514 tig0026649:+22932 Msa1430410:five_prime_UTR
AGTGGTGAGAAAAGGGAAAA+TGG 0.278092 tig0026649:-22736 None:intergenic
AAAGCTCCTACACCAACATT+AGG 0.347170 tig0026649:+22877 Msa1430410:five_prime_UTR
AAGGATTTAAGATCCATCTT+TGG 0.355406 tig0026649:-22978 None:intergenic
GAAGAAGAAGTAATCAATTT+GGG 0.363060 tig0026649:+22933 Msa1430410:five_prime_UTR
AGTAATCAATTTGGGTATGT+TGG 0.388725 tig0026649:+22941 Msa1430410:exon
TGTCGAGAGAGAAGATGAAA+GGG 0.388822 tig0026649:+22780 Msa1430410:five_prime_UTR
ATGGACCAAAAGCAATAATC+AGG 0.422893 tig0026649:+25044 Msa1430410:CDS
TTATGAAGTGGGAAGGCTTA+AGG 0.429941 tig0026649:-22842 None:intergenic
TCTACTGTAGTGGTGAGAAA+AGG 0.444555 tig0026649:-22744 None:intergenic
CAATAATCAGGAAGGAAGTC+TGG 0.445314 tig0026649:+25056 Msa1430410:CDS
AAGCTCCTACACCAACATTA+GGG 0.460989 tig0026649:+22878 Msa1430410:five_prime_UTR
GTGGTGAGAAAAGGGAAAAT+GGG 0.461366 tig0026649:-22735 None:intergenic
CTACTGTAGTGGTGAGAAAA+GGG 0.466765 tig0026649:-22743 None:intergenic
TTTCTGTCTAACCCTAATGT+TGG 0.471425 tig0026649:-22889 None:intergenic
TTGTCGAGAGAGAAGATGAA+AGG 0.497904 tig0026649:+22779 Msa1430410:five_prime_UTR
ACAAACAATTGAAGATATGA+AGG 0.499490 tig0026649:+25089 Msa1430410:CDS
AATCAATTTGGGTATGTTGG+AGG 0.501098 tig0026649:+22944 Msa1430410:exon
CTATGGGGAAATTATGAAGT+GGG 0.502485 tig0026649:-22853 None:intergenic
GGTTGAAGAAGAACCAAAGA+TGG 0.535814 tig0026649:+22965 Msa1430410:CDS
AGGGAAAATGGGTATAGAGT+TGG 0.546792 tig0026649:-22724 None:intergenic
GTCGAGAGAGAAGATGAAAG+GGG 0.569334 tig0026649:+22781 Msa1430410:five_prime_UTR
TCTATGGGGAAATTATGAAG+TGG 0.604070 tig0026649:-22854 None:intergenic
GGGGAAATTATGAAGTGGGA+AGG 0.605746 tig0026649:-22849 None:intergenic
TCTAACCCTAATGTTGGTGT+AGG 0.608709 tig0026649:-22883 None:intergenic
GCTATGCAATGAAATGATAG+AGG 0.613912 tig0026649:-22812 None:intergenic
ACCAAAAGCAATAATCAGGA+AGG 0.620971 tig0026649:+25048 Msa1430410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATATAATTGAGATATTT+AGG + tig0026649:23064-23083 Msa1430410:intron 10.0%
!! TAATATAATTGAGATATTTA+GGG + tig0026649:23065-23084 Msa1430410:intron 10.0%
!! ATAATATAATTGAGATATTT+AGG + tig0026649:23064-23083 Msa1430410:intron 10.0%
!! TAATATAATTGAGATATTTA+GGG + tig0026649:23065-23084 Msa1430410:intron 10.0%
!!! AATGAAATCAAATAATTTTG+TGG - tig0026649:23601-23620 None:intergenic 15.0%
!! TTAAATGATGATTTAATAGA+TGG + tig0026649:23759-23778 Msa1430410:intron 15.0%
!! CAAAAAAAAAAAAAAAGGAA+TGG - tig0026649:23816-23835 None:intergenic 15.0%
!!! TTCCTTTTTTTTTTTTTTTG+AGG + tig0026649:23816-23835 Msa1430410:intron 15.0%
!!! TCCTTTTTTTTTTTTTTTGA+GGG + tig0026649:23817-23836 Msa1430410:intron 15.0%
!!! TTTTTAATGAAGTCTTTTAA+AGG + tig0026649:24664-24683 Msa1430410:intron 15.0%
!!! TTTTAATGAAGTCTTTTAAA+GGG + tig0026649:24665-24684 Msa1430410:intron 15.0%
!! AATAAAGTTTAAACTGAAAT+TGG + tig0026649:24740-24759 Msa1430410:intron 15.0%
!! ATAAAGTTTAAACTGAAATT+GGG + tig0026649:24741-24760 Msa1430410:intron 15.0%
!!! AATGAAATCAAATAATTTTG+TGG - tig0026649:23601-23620 None:intergenic 15.0%
!! TTAAATGATGATTTAATAGA+TGG + tig0026649:23759-23778 Msa1430410:intron 15.0%
!! CAAAAAAAAAAAAAAAGGAA+TGG - tig0026649:23816-23835 None:intergenic 15.0%
!!! TTCCTTTTTTTTTTTTTTTG+AGG + tig0026649:23816-23835 Msa1430410:intron 15.0%
!!! TCCTTTTTTTTTTTTTTTGA+GGG + tig0026649:23817-23836 Msa1430410:intron 15.0%
!!! TTTTTAATGAAGTCTTTTAA+AGG + tig0026649:24664-24683 Msa1430410:intron 15.0%
!!! TTTTAATGAAGTCTTTTAAA+GGG + tig0026649:24665-24684 Msa1430410:intron 15.0%
!! AATAAAGTTTAAACTGAAAT+TGG + tig0026649:24740-24759 Msa1430410:intron 15.0%
!! ATAAAGTTTAAACTGAAATT+GGG + tig0026649:24741-24760 Msa1430410:intron 15.0%
!! GAAATTATATGTTACCTTAA+AGG - tig0026649:23000-23019 None:intergenic 20.0%
!!! ATTTTGCATTTGATTTTGAT+AGG + tig0026649:23145-23164 Msa1430410:intron 20.0%
!!! ACTGATTTGAACTTTAATTT+TGG + tig0026649:23201-23220 Msa1430410:intron 20.0%
!! TTTAGATATAGATAGAAAGA+AGG + tig0026649:23248-23267 Msa1430410:intron 20.0%
!! AAATTCAAGGTAATTAATTC+AGG - tig0026649:23522-23541 None:intergenic 20.0%
!!! AATTACCTTGAATTTTGATA+TGG + tig0026649:23527-23546 Msa1430410:intron 20.0%
!!! ATTACCTTGAATTTTGATAT+GGG + tig0026649:23528-23547 Msa1430410:intron 20.0%
!!! TAATTTTGTGGATTTTACTT+GGG - tig0026649:23589-23608 None:intergenic 20.0%
!!! ATAATTTTGTGGATTTTACT+TGG - tig0026649:23590-23609 None:intergenic 20.0%
!!! TATTTCTAAACTATTCAAAG+TGG + tig0026649:23680-23699 Msa1430410:intron 20.0%
!! TCCCTCAAAAAAAAAAAAAA+AGG - tig0026649:23821-23840 None:intergenic 20.0%
!! TCCTTATTAAAGAGTTATAA+TGG + tig0026649:23909-23928 Msa1430410:intron 20.0%
!!! CATTCTTAGTTTCAAAATTT+TGG - tig0026649:24999-25018 None:intergenic 20.0%
!! GAAATTATATGTTACCTTAA+AGG - tig0026649:23000-23019 None:intergenic 20.0%
!!! ATTTTGCATTTGATTTTGAT+AGG + tig0026649:23145-23164 Msa1430410:intron 20.0%
!!! ACTGATTTGAACTTTAATTT+TGG + tig0026649:23201-23220 Msa1430410:intron 20.0%
!! TTTAGATATAGATAGAAAGA+AGG + tig0026649:23248-23267 Msa1430410:intron 20.0%
!! AAATTCAAGGTAATTAATTC+AGG - tig0026649:23522-23541 None:intergenic 20.0%
!!! AATTACCTTGAATTTTGATA+TGG + tig0026649:23527-23546 Msa1430410:intron 20.0%
!!! ATTACCTTGAATTTTGATAT+GGG + tig0026649:23528-23547 Msa1430410:intron 20.0%
!!! TAATTTTGTGGATTTTACTT+GGG - tig0026649:23589-23608 None:intergenic 20.0%
!!! ATAATTTTGTGGATTTTACT+TGG - tig0026649:23590-23609 None:intergenic 20.0%
!!! TATTTCTAAACTATTCAAAG+TGG + tig0026649:23680-23699 Msa1430410:intron 20.0%
!! TCCCTCAAAAAAAAAAAAAA+AGG - tig0026649:23821-23840 None:intergenic 20.0%
!! TCCTTATTAAAGAGTTATAA+TGG + tig0026649:23909-23928 Msa1430410:intron 20.0%
!!! CATTCTTAGTTTCAAAATTT+TGG - tig0026649:24999-25018 None:intergenic 20.0%
! AGAAGAAGAAGTAATCAATT+TGG + tig0026649:22932-22951 Msa1430410:five_prime_UTR 25.0%
! GAAGAAGAAGTAATCAATTT+GGG + tig0026649:22933-22952 Msa1430410:five_prime_UTR 25.0%
!!! AGTTGATTTGATTGATTGTT+TGG + tig0026649:23100-23119 Msa1430410:intron 25.0%
! ATTTGATTGATTGTTTGGTT+TGG + tig0026649:23105-23124 Msa1430410:intron 25.0%
!! GGTGACTTTTAATAACTTAT+TGG + tig0026649:23327-23346 Msa1430410:intron 25.0%
! CACGACAAAAATAACTAAAA+CGG - tig0026649:23789-23808 None:intergenic 25.0%
! GCCATTATAACTCTTTAATA+AGG - tig0026649:23913-23932 None:intergenic 25.0%
!!! CATAATAGTTTTGTGATGTA+TGG + tig0026649:23932-23951 Msa1430410:intron 25.0%
!!! GTTTTTGAGATTTAATGAGA+AGG + tig0026649:24404-24423 Msa1430410:intron 25.0%
!! AGTTTTACTCACTATGTATT+TGG + tig0026649:24534-24553 Msa1430410:intron 25.0%
!! GTTTTACTCACTATGTATTT+GGG + tig0026649:24535-24554 Msa1430410:intron 25.0%
!! TTTTACTCACTATGTATTTG+GGG + tig0026649:24536-24555 Msa1430410:intron 25.0%
! AGAAGAAGAAGTAATCAATT+TGG + tig0026649:22932-22951 Msa1430410:five_prime_UTR 25.0%
! GAAGAAGAAGTAATCAATTT+GGG + tig0026649:22933-22952 Msa1430410:five_prime_UTR 25.0%
!!! AGTTGATTTGATTGATTGTT+TGG + tig0026649:23100-23119 Msa1430410:intron 25.0%
! ATTTGATTGATTGTTTGGTT+TGG + tig0026649:23105-23124 Msa1430410:intron 25.0%
!! GGTGACTTTTAATAACTTAT+TGG + tig0026649:23327-23346 Msa1430410:intron 25.0%
! CACGACAAAAATAACTAAAA+CGG - tig0026649:23789-23808 None:intergenic 25.0%
! GCCATTATAACTCTTTAATA+AGG - tig0026649:23913-23932 None:intergenic 25.0%
!!! CATAATAGTTTTGTGATGTA+TGG + tig0026649:23932-23951 Msa1430410:intron 25.0%
!!! GTTTTTGAGATTTAATGAGA+AGG + tig0026649:24404-24423 Msa1430410:intron 25.0%
!! AGTTTTACTCACTATGTATT+TGG + tig0026649:24534-24553 Msa1430410:intron 25.0%
!! GTTTTACTCACTATGTATTT+GGG + tig0026649:24535-24554 Msa1430410:intron 25.0%
!! TTTTACTCACTATGTATTTG+GGG + tig0026649:24536-24555 Msa1430410:intron 25.0%
AGTAATCAATTTGGGTATGT+TGG + tig0026649:22941-22960 Msa1430410:exon 30.0%
! AAGGATTTAAGATCCATCTT+TGG - tig0026649:22981-23000 None:intergenic 30.0%
GATGGATCTTAAATCCTTTA+AGG + tig0026649:22983-23002 Msa1430410:CDS 30.0%
! ATGTTTGTGCAGAAATTTTC+AGG - tig0026649:23299-23318 None:intergenic 30.0%
ATTGGCATGTGATATTCATA+TGG + tig0026649:23345-23364 Msa1430410:intron 30.0%
TTGGCATGTGATATTCATAT+GGG + tig0026649:23346-23365 Msa1430410:intron 30.0%
!! TGCATTAGTCTTATGTTCAT+TGG + tig0026649:23478-23497 Msa1430410:intron 30.0%
TGTTCCCATATCAAAATTCA+AGG - tig0026649:23535-23554 None:intergenic 30.0%
! AATTGTCTTTGTTTGTGAAC+TGG + tig0026649:23559-23578 Msa1430410:intron 30.0%
CTTTCTATAATCATTGCAAG+AGG + tig0026649:23715-23734 Msa1430410:intron 30.0%
!!! ATAGTTTTGTGATGTATGGT+TGG + tig0026649:23936-23955 Msa1430410:intron 30.0%
! GATGTATGGTTGGTTATTAA+AGG + tig0026649:23946-23965 Msa1430410:intron 30.0%
TGGTTATTAAAGGTTGAAGA+AGG + tig0026649:23956-23975 Msa1430410:intron 30.0%
! ACTTTTCTCATGTCATTGAA+CGG - tig0026649:24017-24036 None:intergenic 30.0%
TATTAAGATTTACCCTTGCA+AGG + tig0026649:24177-24196 Msa1430410:intron 30.0%
!!! TTCATAGTTTTAACCTCTTC+TGG - tig0026649:24279-24298 None:intergenic 30.0%
TCATTAAAAACTCTAGACTC+TGG - tig0026649:24654-24673 None:intergenic 30.0%
CTTCTCTTACTAAAAGAAGT+GGG + tig0026649:24829-24848 Msa1430410:intron 30.0%
TTCTCTTACTAAAAGAAGTG+GGG + tig0026649:24830-24849 Msa1430410:intron 30.0%
TGCCAATTCATAATTCAGAA+TGG + tig0026649:25025-25044 Msa1430410:intron 30.0%
GTCCATTCTGAATTATGAAT+TGG - tig0026649:25030-25049 None:intergenic 30.0%
AGTAATCAATTTGGGTATGT+TGG + tig0026649:22941-22960 Msa1430410:exon 30.0%
! AAGGATTTAAGATCCATCTT+TGG - tig0026649:22981-23000 None:intergenic 30.0%
GATGGATCTTAAATCCTTTA+AGG + tig0026649:22983-23002 Msa1430410:CDS 30.0%
! ATGTTTGTGCAGAAATTTTC+AGG - tig0026649:23299-23318 None:intergenic 30.0%
ATTGGCATGTGATATTCATA+TGG + tig0026649:23345-23364 Msa1430410:intron 30.0%
TTGGCATGTGATATTCATAT+GGG + tig0026649:23346-23365 Msa1430410:intron 30.0%
!! TGCATTAGTCTTATGTTCAT+TGG + tig0026649:23478-23497 Msa1430410:intron 30.0%
TGTTCCCATATCAAAATTCA+AGG - tig0026649:23535-23554 None:intergenic 30.0%
! AATTGTCTTTGTTTGTGAAC+TGG + tig0026649:23559-23578 Msa1430410:intron 30.0%
CTTTCTATAATCATTGCAAG+AGG + tig0026649:23715-23734 Msa1430410:intron 30.0%
!!! ATAGTTTTGTGATGTATGGT+TGG + tig0026649:23936-23955 Msa1430410:intron 30.0%
! GATGTATGGTTGGTTATTAA+AGG + tig0026649:23946-23965 Msa1430410:intron 30.0%
TGGTTATTAAAGGTTGAAGA+AGG + tig0026649:23956-23975 Msa1430410:intron 30.0%
! ACTTTTCTCATGTCATTGAA+CGG - tig0026649:24017-24036 None:intergenic 30.0%
TATTAAGATTTACCCTTGCA+AGG + tig0026649:24177-24196 Msa1430410:intron 30.0%
!!! TTCATAGTTTTAACCTCTTC+TGG - tig0026649:24279-24298 None:intergenic 30.0%
TCATTAAAAACTCTAGACTC+TGG - tig0026649:24654-24673 None:intergenic 30.0%
CTTCTCTTACTAAAAGAAGT+GGG + tig0026649:24829-24848 Msa1430410:intron 30.0%
TTCTCTTACTAAAAGAAGTG+GGG + tig0026649:24830-24849 Msa1430410:intron 30.0%
TGCCAATTCATAATTCAGAA+TGG + tig0026649:25025-25044 Msa1430410:intron 30.0%
GTCCATTCTGAATTATGAAT+TGG - tig0026649:25030-25049 None:intergenic 30.0%
GCTATGCAATGAAATGATAG+AGG - tig0026649:22815-22834 None:intergenic 35.0%
CTATGGGGAAATTATGAAGT+GGG - tig0026649:22856-22875 None:intergenic 35.0%
TCTATGGGGAAATTATGAAG+TGG - tig0026649:22857-22876 None:intergenic 35.0%
TTTCTGTCTAACCCTAATGT+TGG - tig0026649:22892-22911 None:intergenic 35.0%
AATCAATTTGGGTATGTTGG+AGG + tig0026649:22944-22963 Msa1430410:exon 35.0%
TTCTCTTCTTAAACCTCTTC+AGG + tig0026649:23017-23036 Msa1430410:intron 35.0%
TCTCTTCTTAAACCTCTTCA+GGG + tig0026649:23018-23037 Msa1430410:intron 35.0%
AGGGTGTGCTTATAAATTGT+TGG + tig0026649:23037-23056 Msa1430410:intron 35.0%
GAAGATAGTCAACTTGCATA+TGG - tig0026649:23388-23407 None:intergenic 35.0%
CTATTCAAAGTGGTGAATCA+TGG + tig0026649:23690-23709 Msa1430410:intron 35.0%
TAACCATTCCTAAAGAACAG+AGG + tig0026649:23847-23866 Msa1430410:intron 35.0%
! AAACCTCTGTTCTTTAGGAA+TGG - tig0026649:23853-23872 None:intergenic 35.0%
TTCTCAAACCTCTGTTCTTT+AGG - tig0026649:23858-23877 None:intergenic 35.0%
AACACGTTTAATAGCGTGAA+TGG + tig0026649:24055-24074 Msa1430410:intron 35.0%
TTAACCTCTTCTGGTAAGAT+TGG - tig0026649:24270-24289 None:intergenic 35.0%
TAAAACGTTTCCAACCATGT+CGG - tig0026649:24484-24503 None:intergenic 35.0%
GCTTCTCTTACTAAAAGAAG+TGG + tig0026649:24828-24847 Msa1430410:intron 35.0%
!!! ACATTTTTGAAACCCTCACT+CGG - tig0026649:24878-24897 None:intergenic 35.0%
AACAATTAAGCCATCTGATG+TGG + tig0026649:24904-24923 Msa1430410:intron 35.0%
GCTAATGTGGATAAGGAATT+AGG + tig0026649:24972-24991 Msa1430410:intron 35.0%
ATGGACCAAAAGCAATAATC+AGG + tig0026649:25044-25063 Msa1430410:CDS 35.0%
ACCAAAAGCAATAATCAGGA+AGG + tig0026649:25048-25067 Msa1430410:CDS 35.0%
! TCCTTCCTGATTATTGCTTT+TGG - tig0026649:25052-25071 None:intergenic 35.0%
GCTATGCAATGAAATGATAG+AGG - tig0026649:22815-22834 None:intergenic 35.0%
CTATGGGGAAATTATGAAGT+GGG - tig0026649:22856-22875 None:intergenic 35.0%
TCTATGGGGAAATTATGAAG+TGG - tig0026649:22857-22876 None:intergenic 35.0%
TTTCTGTCTAACCCTAATGT+TGG - tig0026649:22892-22911 None:intergenic 35.0%
AATCAATTTGGGTATGTTGG+AGG + tig0026649:22944-22963 Msa1430410:exon 35.0%
TTCTCTTCTTAAACCTCTTC+AGG + tig0026649:23017-23036 Msa1430410:intron 35.0%
TCTCTTCTTAAACCTCTTCA+GGG + tig0026649:23018-23037 Msa1430410:intron 35.0%
AGGGTGTGCTTATAAATTGT+TGG + tig0026649:23037-23056 Msa1430410:intron 35.0%
GAAGATAGTCAACTTGCATA+TGG - tig0026649:23388-23407 None:intergenic 35.0%
CTATTCAAAGTGGTGAATCA+TGG + tig0026649:23690-23709 Msa1430410:intron 35.0%
TAACCATTCCTAAAGAACAG+AGG + tig0026649:23847-23866 Msa1430410:intron 35.0%
! AAACCTCTGTTCTTTAGGAA+TGG - tig0026649:23853-23872 None:intergenic 35.0%
TTCTCAAACCTCTGTTCTTT+AGG - tig0026649:23858-23877 None:intergenic 35.0%
AACACGTTTAATAGCGTGAA+TGG + tig0026649:24055-24074 Msa1430410:intron 35.0%
TTAACCTCTTCTGGTAAGAT+TGG - tig0026649:24270-24289 None:intergenic 35.0%
TAAAACGTTTCCAACCATGT+CGG - tig0026649:24484-24503 None:intergenic 35.0%
GCTTCTCTTACTAAAAGAAG+TGG + tig0026649:24828-24847 Msa1430410:intron 35.0%
!!! ACATTTTTGAAACCCTCACT+CGG - tig0026649:24878-24897 None:intergenic 35.0%
AACAATTAAGCCATCTGATG+TGG + tig0026649:24904-24923 Msa1430410:intron 35.0%
GCTAATGTGGATAAGGAATT+AGG + tig0026649:24972-24991 Msa1430410:intron 35.0%
ATGGACCAAAAGCAATAATC+AGG + tig0026649:25044-25063 Msa1430410:CDS 35.0%
ACCAAAAGCAATAATCAGGA+AGG + tig0026649:25048-25067 Msa1430410:CDS 35.0%
! TCCTTCCTGATTATTGCTTT+TGG - tig0026649:25052-25071 None:intergenic 35.0%
GTGGTGAGAAAAGGGAAAAT+GGG - tig0026649:22738-22757 None:intergenic 40.0%
AGTGGTGAGAAAAGGGAAAA+TGG - tig0026649:22739-22758 None:intergenic 40.0%
CTACTGTAGTGGTGAGAAAA+GGG - tig0026649:22746-22765 None:intergenic 40.0%
TCTACTGTAGTGGTGAGAAA+AGG - tig0026649:22747-22766 None:intergenic 40.0%
TTGTCGAGAGAGAAGATGAA+AGG + tig0026649:22779-22798 Msa1430410:five_prime_UTR 40.0%
TGTCGAGAGAGAAGATGAAA+GGG + tig0026649:22780-22799 Msa1430410:five_prime_UTR 40.0%
TTATGAAGTGGGAAGGCTTA+AGG - tig0026649:22845-22864 None:intergenic 40.0%
! TGGTGTAGGAGCTTTTCTAT+GGG - tig0026649:22872-22891 None:intergenic 40.0%
! TTGGTGTAGGAGCTTTTCTA+TGG - tig0026649:22873-22892 None:intergenic 40.0%
AAAGCTCCTACACCAACATT+AGG + tig0026649:22877-22896 Msa1430410:five_prime_UTR 40.0%
AAGCTCCTACACCAACATTA+GGG + tig0026649:22878-22897 Msa1430410:five_prime_UTR 40.0%
TCTAACCCTAATGTTGGTGT+AGG - tig0026649:22886-22905 None:intergenic 40.0%
GGTTGAAGAAGAACCAAAGA+TGG + tig0026649:22965-22984 Msa1430410:CDS 40.0%
TTTATAAGCACACCCTGAAG+AGG - tig0026649:23033-23052 None:intergenic 40.0%
TCTGCACAAACATGTTCAGT+TGG + tig0026649:23306-23325 Msa1430410:intron 40.0%
AGTGCTCCGTCTATATATGA+CGG + tig0026649:24202-24221 Msa1430410:intron 40.0%
CATGTCCCGTCATATATAGA+CGG - tig0026649:24211-24230 None:intergenic 40.0%
ATCTCCAATCTTACCAGAAG+AGG + tig0026649:24263-24282 Msa1430410:intron 40.0%
CACACTTAGTGACTTAGACA+AGG + tig0026649:24348-24367 Msa1430410:intron 40.0%
ACTTAGTGACTTAGACAAGG+AGG + tig0026649:24351-24370 Msa1430410:intron 40.0%
CTAATGTGCACTTCTATACC+TGG - tig0026649:24570-24589 None:intergenic 40.0%
TGTGCATGCAAATGATCTGA+TGG + tig0026649:24781-24800 Msa1430410:intron 40.0%
AATTAAGCCATCTGATGTGG+AGG + tig0026649:24907-24926 Msa1430410:intron 40.0%
ATTAAGCCATCTGATGTGGA+GGG + tig0026649:24908-24927 Msa1430410:intron 40.0%
CAATAATCAGGAAGGAAGTC+TGG + tig0026649:25056-25075 Msa1430410:CDS 40.0%
GTGGTGAGAAAAGGGAAAAT+GGG - tig0026649:22738-22757 None:intergenic 40.0%
AGTGGTGAGAAAAGGGAAAA+TGG - tig0026649:22739-22758 None:intergenic 40.0%
CTACTGTAGTGGTGAGAAAA+GGG - tig0026649:22746-22765 None:intergenic 40.0%
TCTACTGTAGTGGTGAGAAA+AGG - tig0026649:22747-22766 None:intergenic 40.0%
TTGTCGAGAGAGAAGATGAA+AGG + tig0026649:22779-22798 Msa1430410:five_prime_UTR 40.0%
TGTCGAGAGAGAAGATGAAA+GGG + tig0026649:22780-22799 Msa1430410:five_prime_UTR 40.0%
TTATGAAGTGGGAAGGCTTA+AGG - tig0026649:22845-22864 None:intergenic 40.0%
! TGGTGTAGGAGCTTTTCTAT+GGG - tig0026649:22872-22891 None:intergenic 40.0%
! TTGGTGTAGGAGCTTTTCTA+TGG - tig0026649:22873-22892 None:intergenic 40.0%
AAAGCTCCTACACCAACATT+AGG + tig0026649:22877-22896 Msa1430410:five_prime_UTR 40.0%
AAGCTCCTACACCAACATTA+GGG + tig0026649:22878-22897 Msa1430410:five_prime_UTR 40.0%
TCTAACCCTAATGTTGGTGT+AGG - tig0026649:22886-22905 None:intergenic 40.0%
GGTTGAAGAAGAACCAAAGA+TGG + tig0026649:22965-22984 Msa1430410:CDS 40.0%
TTTATAAGCACACCCTGAAG+AGG - tig0026649:23033-23052 None:intergenic 40.0%
TCTGCACAAACATGTTCAGT+TGG + tig0026649:23306-23325 Msa1430410:intron 40.0%
AGTGCTCCGTCTATATATGA+CGG + tig0026649:24202-24221 Msa1430410:intron 40.0%
CATGTCCCGTCATATATAGA+CGG - tig0026649:24211-24230 None:intergenic 40.0%
ATCTCCAATCTTACCAGAAG+AGG + tig0026649:24263-24282 Msa1430410:intron 40.0%
CACACTTAGTGACTTAGACA+AGG + tig0026649:24348-24367 Msa1430410:intron 40.0%
ACTTAGTGACTTAGACAAGG+AGG + tig0026649:24351-24370 Msa1430410:intron 40.0%
CTAATGTGCACTTCTATACC+TGG - tig0026649:24570-24589 None:intergenic 40.0%
TGTGCATGCAAATGATCTGA+TGG + tig0026649:24781-24800 Msa1430410:intron 40.0%
AATTAAGCCATCTGATGTGG+AGG + tig0026649:24907-24926 Msa1430410:intron 40.0%
ATTAAGCCATCTGATGTGGA+GGG + tig0026649:24908-24927 Msa1430410:intron 40.0%
CAATAATCAGGAAGGAAGTC+TGG + tig0026649:25056-25075 Msa1430410:CDS 40.0%
TCTCTCTCTCTCTACTGTAG+TGG - tig0026649:22757-22776 None:intergenic 45.0%
GTCGAGAGAGAAGATGAAAG+GGG + tig0026649:22781-22800 Msa1430410:five_prime_UTR 45.0%
GGGGAAATTATGAAGTGGGA+AGG - tig0026649:22852-22871 None:intergenic 45.0%
! GGTGTAGGAGCTTTTCTATG+GGG - tig0026649:22871-22890 None:intergenic 45.0%
GCATATGGACACGATGCAAT+TGG - tig0026649:23373-23392 None:intergenic 45.0%
GTGCTCCGTCTATATATGAC+GGG + tig0026649:24203-24222 Msa1430410:intron 45.0%
ACATGTAAGCGAGGGTAAAC+AGG + tig0026649:24226-24245 Msa1430410:intron 45.0%
CATGTAAGCGAGGGTAAACA+GGG + tig0026649:24227-24246 Msa1430410:intron 45.0%
! GTATTTGGGGTTACGTTTCC+AGG + tig0026649:24549-24568 Msa1430410:intron 45.0%
TCAGCCGTAAATCGTTCAGT+CGG + tig0026649:24615-24634 Msa1430410:intron 45.0%
TCTAGACTCTGGACGTGATT+CGG - tig0026649:24643-24662 None:intergenic 45.0%
CACTCGGAGCAAGATTCTAA+TGG - tig0026649:24862-24881 None:intergenic 45.0%
TTAGAATCTTGCTCCGAGTG+AGG + tig0026649:24862-24881 Msa1430410:intron 45.0%
TAGAATCTTGCTCCGAGTGA+GGG + tig0026649:24863-24882 Msa1430410:intron 45.0%
TCTCTCTCTCTCTACTGTAG+TGG - tig0026649:22757-22776 None:intergenic 45.0%
GTCGAGAGAGAAGATGAAAG+GGG + tig0026649:22781-22800 Msa1430410:five_prime_UTR 45.0%
GGGGAAATTATGAAGTGGGA+AGG - tig0026649:22852-22871 None:intergenic 45.0%
! GGTGTAGGAGCTTTTCTATG+GGG - tig0026649:22871-22890 None:intergenic 45.0%
GCATATGGACACGATGCAAT+TGG - tig0026649:23373-23392 None:intergenic 45.0%
GTGCTCCGTCTATATATGAC+GGG + tig0026649:24203-24222 Msa1430410:intron 45.0%
ACATGTAAGCGAGGGTAAAC+AGG + tig0026649:24226-24245 Msa1430410:intron 45.0%
CATGTAAGCGAGGGTAAACA+GGG + tig0026649:24227-24246 Msa1430410:intron 45.0%
! GTATTTGGGGTTACGTTTCC+AGG + tig0026649:24549-24568 Msa1430410:intron 45.0%
TCAGCCGTAAATCGTTCAGT+CGG + tig0026649:24615-24634 Msa1430410:intron 45.0%
TCTAGACTCTGGACGTGATT+CGG - tig0026649:24643-24662 None:intergenic 45.0%
CACTCGGAGCAAGATTCTAA+TGG - tig0026649:24862-24881 None:intergenic 45.0%
TTAGAATCTTGCTCCGAGTG+AGG + tig0026649:24862-24881 Msa1430410:intron 45.0%
TAGAATCTTGCTCCGAGTGA+GGG + tig0026649:24863-24882 Msa1430410:intron 45.0%
! TATGACGGGACATGTAAGCG+AGG + tig0026649:24217-24236 Msa1430410:intron 50.0%
! ATGACGGGACATGTAAGCGA+GGG + tig0026649:24218-24237 Msa1430410:intron 50.0%
CCATGTCGGTGTGCTAAACA+TGG - tig0026649:24470-24489 None:intergenic 50.0%
CCATGTTTAGCACACCGACA+TGG + tig0026649:24467-24486 Msa1430410:intron 50.0%
GTTTAGCACACCGACATGGT+TGG + tig0026649:24471-24490 Msa1430410:intron 50.0%
GGCTCCGACTGAACGATTTA+CGG - tig0026649:24622-24641 None:intergenic 50.0%
CTTTCTCCCTCCACATCAGA+TGG - tig0026649:24917-24936 None:intergenic 50.0%
! ACATTAGCCTCCCCAAACTC+GGG - tig0026649:24960-24979 None:intergenic 50.0%
! CACATTAGCCTCCCCAAACT+CGG - tig0026649:24961-24980 None:intergenic 50.0%
TGGGGAGGCTAATGTGGATA+AGG + tig0026649:24965-24984 Msa1430410:intron 50.0%
! TATGACGGGACATGTAAGCG+AGG + tig0026649:24217-24236 Msa1430410:intron 50.0%
! ATGACGGGACATGTAAGCGA+GGG + tig0026649:24218-24237 Msa1430410:intron 50.0%
CCATGTCGGTGTGCTAAACA+TGG - tig0026649:24470-24489 None:intergenic 50.0%
CCATGTTTAGCACACCGACA+TGG + tig0026649:24467-24486 Msa1430410:intron 50.0%
GTTTAGCACACCGACATGGT+TGG + tig0026649:24471-24490 Msa1430410:intron 50.0%
GGCTCCGACTGAACGATTTA+CGG - tig0026649:24622-24641 None:intergenic 50.0%
CTTTCTCCCTCCACATCAGA+TGG - tig0026649:24917-24936 None:intergenic 50.0%
! ACATTAGCCTCCCCAAACTC+GGG - tig0026649:24960-24979 None:intergenic 50.0%
! CACATTAGCCTCCCCAAACT+CGG - tig0026649:24961-24980 None:intergenic 50.0%
TGGGGAGGCTAATGTGGATA+AGG + tig0026649:24965-24984 Msa1430410:intron 50.0%
! ACGGAGCACTCACCTTGCAA+GGG - tig0026649:24192-24211 None:intergenic 55.0%
TTAGTCCCGACTCCCGAGTT+TGG + tig0026649:24945-24964 Msa1430410:intron 55.0%
TAGTCCCGACTCCCGAGTTT+GGG + tig0026649:24946-24965 Msa1430410:intron 55.0%
CGAGTTTGGGGAGGCTAATG+TGG + tig0026649:24959-24978 Msa1430410:intron 55.0%
! ACGGAGCACTCACCTTGCAA+GGG - tig0026649:24192-24211 None:intergenic 55.0%
TTAGTCCCGACTCCCGAGTT+TGG + tig0026649:24945-24964 Msa1430410:intron 55.0%
TAGTCCCGACTCCCGAGTTT+GGG + tig0026649:24946-24965 Msa1430410:intron 55.0%
CGAGTTTGGGGAGGCTAATG+TGG + tig0026649:24959-24978 Msa1430410:intron 55.0%
! GACGGAGCACTCACCTTGCA+AGG - tig0026649:24193-24212 None:intergenic 60.0%
CGGGAGTCGGGACTAACTAC+TGG - tig0026649:24941-24960 None:intergenic 60.0%
AGTCCCGACTCCCGAGTTTG+GGG + tig0026649:24947-24966 Msa1430410:intron 60.0%
! GACGGAGCACTCACCTTGCA+AGG - tig0026649:24193-24212 None:intergenic 60.0%
CGGGAGTCGGGACTAACTAC+TGG - tig0026649:24941-24960 None:intergenic 60.0%
AGTCCCGACTCCCGAGTTTG+GGG + tig0026649:24947-24966 Msa1430410:intron 60.0%
CCTCCCCAAACTCGGGAGTC+GGG - tig0026649:24953-24972 None:intergenic 65.0%
GCCTCCCCAAACTCGGGAGT+CGG - tig0026649:24954-24973 None:intergenic 65.0%
CCTCCCCAAACTCGGGAGTC+GGG - tig0026649:24953-24972 None:intergenic 65.0%
GCCTCCCCAAACTCGGGAGT+CGG - tig0026649:24954-24973 None:intergenic 65.0%
CCCGACTCCCGAGTTTGGGG+AGG + tig0026649:24950-24969 Msa1430410:intron 70.0%
CCCGACTCCCGAGTTTGGGG+AGG + tig0026649:24950-24969 Msa1430410:intron 70.0%
Chromosome Type Strat End Strand Name
tig0026649 gene 22725 25108 22725 ID=Msa1430410;Name=Msa1430410
tig0026649 mRNA 22725 25108 22725 ID=Msa1430410-mRNA-1;Parent=Msa1430410;Name=Msa1430410-mRNA-1;_AED=0.38;_eAED=0.49;_QI=232|0|0.5|1|0|0.5|2|0|37
tig0026649 exon 22725 23004 22725 ID=Msa1430410-mRNA-1:exon:6979;Parent=Msa1430410-mRNA-1
tig0026649 exon 25043 25108 25043 ID=Msa1430410-mRNA-1:exon:6980;Parent=Msa1430410-mRNA-1
tig0026649 five_prime_UTR 22725 22956 22725 ID=Msa1430410-mRNA-1:five_prime_utr;Parent=Msa1430410-mRNA-1
tig0026649 CDS 22957 23004 22957 ID=Msa1430410-mRNA-1:cds;Parent=Msa1430410-mRNA-1
tig0026649 CDS 25043 25108 25043 ID=Msa1430410-mRNA-1:cds;Parent=Msa1430410-mRNA-1
Gene Sequence

>Msa1430410

ATGTTGGAGGTTGAAGAAGAACCAAAGATGGATCTTAAATCCTTTAAGAATGGACCAAAAGCAATAATCAGGAAGGAAGTCTGGCAGCGTAGAAACAAACAATTGAAGATATGA
Protein sequence

>Msa1430410

MLEVEEEPKMDLKSFKNGPKAIIRKEVWQRRNKQLKI*