AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1001180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1001180 MtrunA17_Chr8g0386311 76.923 39 9 0 5 43 44 82 2.19e-12 59.7
Msa1001180 MtrunA17_Chr3g0132111 76.923 39 9 0 5 43 43 81 1.32e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 74 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTGAATTTACTTATTT+TGG 0.039273 6_4:+84873141 Msa1001180:five_prime_UTR
CTGATAACTTTGTTTCTTCT+TGG 0.158025 6_4:+84875288 Msa1001180:five_prime_UTR
GAAATGATCCGTAGAAAATT+TGG 0.165227 6_4:-84871599 None:intergenic
TACTTGTGTATTCATCTTAA+TGG 0.165582 6_4:-84874537 None:intergenic
ACTTTGTTTCTTCTTGGTTT+TGG 0.183733 6_4:+84875294 Msa1001180:five_prime_UTR
AAAGGACGAGATTGGGGTTT+TGG 0.236133 6_4:-84871744 None:intergenic
AATAACCTTTCTTGGACTTT+TGG 0.248669 6_4:+84874710 Msa1001180:five_prime_UTR
AAAGTTTCTCATTGACTTAC+TGG 0.250376 6_4:-84871632 None:intergenic
GCATCAGGTGTATGTTTAAA+AGG 0.258580 6_4:-84874658 None:intergenic
AAACAGAAGTCACCCTTAAA+TGG 0.277775 6_4:+84874346 Msa1001180:five_prime_UTR
GCCACCAAAAGCTTTGTTTC+CGG 0.296395 6_4:+84871798 Msa1001180:five_prime_UTR
GTTTGAATTCGTTATTCTAT+AGG 0.319565 6_4:+84871889 Msa1001180:five_prime_UTR
GTAAGTATAATTTAACAAAA+AGG 0.323622 6_4:-84874953 None:intergenic
ATTGTTCTTACTTACAAATC+AGG 0.329636 6_4:-84874575 None:intergenic
TGGTGGTAAATAAGTCTAAA+TGG 0.335165 6_4:+84875157 Msa1001180:five_prime_UTR
TATTGGATGTGGTTGAGTTT+GGG 0.357152 6_4:+84876487 Msa1001180:CDS
GTATTGGATGTGGTTGAGTT+TGG 0.369084 6_4:+84876486 Msa1001180:intron
AAATGATCCGTAGAAAATTT+GGG 0.369086 6_4:-84871598 None:intergenic
AGAGGGAGGTGGCTGCCGTT+AGG 0.384771 6_4:-84871676 None:intergenic
GCGACTTTCAAAGGCATTGA+TGG 0.384786 6_4:+84875346 Msa1001180:CDS
CAAAGGCAAAGGACGAGATT+GGG 0.389709 6_4:-84871751 None:intergenic
TCTCCAAAATAAGTAAATTC+AGG 0.412247 6_4:-84873144 None:intergenic
GAGGGAGGTGGCTGCCGTTA+GGG 0.419184 6_4:-84871675 None:intergenic
TGATGCATTTGATTATTATG+AGG 0.419988 6_4:+84874675 Msa1001180:five_prime_UTR
GTGACCGGAAACAAAGCTTT+TGG 0.424992 6_4:-84871802 None:intergenic
CTCATTGACTTACTGGGATG+TGG 0.430914 6_4:-84871625 None:intergenic
ATTTAGAAATTCGGTAGAAA+AGG 0.435041 6_4:-84876577 None:intergenic
TCAAAGGCAAAGGACGAGAT+TGG 0.435552 6_4:-84871752 None:intergenic
TGGATATAACTTGCTGGTGT+GGG 0.459628 6_4:+84874830 Msa1001180:five_prime_UTR
TCATAATAATCAAATGCATC+AGG 0.467029 6_4:-84874673 None:intergenic
TTGAAAGTCGCAATGATGTC+AGG 0.469232 6_4:-84875334 None:intergenic
GTAAATCAACGATTCTTGAA+TGG 0.470651 6_4:+84874739 Msa1001180:five_prime_UTR
CAGATCTGCTGCTAACTTCG+TGG 0.476295 6_4:-84871837 None:intergenic
TTCAAACATACTTTATGACT+TGG 0.484055 6_4:+84875087 Msa1001180:five_prime_UTR
GACATCATTGCGACTTTCAA+AGG 0.493983 6_4:+84875337 Msa1001180:CDS
TTGGATATAACTTGCTGGTG+TGG 0.496418 6_4:+84874829 Msa1001180:five_prime_UTR
CTATTTCTAACTGATTCAAA+TGG 0.504392 6_4:+84875431 Msa1001180:CDS
TTTGTTAACAGTATTGGATG+TGG 0.516356 6_4:+84876476 Msa1001180:intron
ATACAAACTAACACGGATGA+AGG 0.522459 6_4:-84874425 None:intergenic
AAACACTGACTTCGTTGTTG+TGG 0.535015 6_4:-84871870 None:intergenic
AAGTTTCTCATTGACTTACT+GGG 0.535577 6_4:-84871631 None:intergenic
TGGATGAGTGTGAGGGTGAC+CGG 0.543608 6_4:-84871817 None:intergenic
GATTACCACTTACAAAATCA+AGG 0.544502 6_4:+84873165 Msa1001180:five_prime_UTR
ATTGGATGTGGTTGAGTTTG+GGG 0.545293 6_4:+84876488 Msa1001180:CDS
GTGTGGCACGACAAAGGAAG+AGG 0.545614 6_4:-84871694 None:intergenic
AAAGGGAACTTACATGATGA+AGG 0.546474 6_4:-84872376 None:intergenic
TTACAAATCAGGATCAAAAG+TGG 0.548977 6_4:-84874564 None:intergenic
AGCTCAACCATGGAGTTTAG+CGG 0.551609 6_4:+84872342 Msa1001180:five_prime_UTR
GTAGAAAAGGAAAACTTACA+TGG 0.552184 6_4:-84876564 None:intergenic
GGACATCAAATTCGTGTCAA+AGG 0.552949 6_4:-84871768 None:intergenic
GTTAAATTATACTTACACCT+TGG 0.554529 6_4:+84874960 Msa1001180:five_prime_UTR
ACACACCAAAAGTCCAAGAA+AGG 0.557195 6_4:-84874715 None:intergenic
GTCACTTTCAAGCTCAACCA+TGG 0.563416 6_4:+84872332 Msa1001180:five_prime_UTR
TTATGACTTGGCTGTACATG+AGG 0.568386 6_4:+84875099 Msa1001180:five_prime_UTR
CAAATTCGTGTCAAAGGCAA+AGG 0.576702 6_4:-84871762 None:intergenic
ACGACAAAGGAAGAGGGAGG+TGG 0.581715 6_4:-84871687 None:intergenic
AGATCTGCCGCTAAACTCCA+TGG 0.584643 6_4:-84872349 None:intergenic
AAAGGCAAAGGACGAGATTG+GGG 0.595795 6_4:-84871750 None:intergenic
TAACTTCGTGGATGAGTGTG+AGG 0.596719 6_4:-84871825 None:intergenic
AATTCGTTATTCTATAGGCA+CGG 0.618899 6_4:+84871894 Msa1001180:five_prime_UTR
TTTACAGTGTGGCACGACAA+AGG 0.625017 6_4:-84871700 None:intergenic
TCTCCCCTCGAAAACCCTAA+CGG 0.628961 6_4:+84871661 Msa1001180:five_prime_UTR
CTAGAAGCAAGAGTTCTGCA+AGG 0.634150 6_4:+84876516 Msa1001180:CDS
GCTTGCTATCTGCCTCCATG+TGG 0.636328 6_4:+84874384 Msa1001180:five_prime_UTR
AAAATGTTAATAGAAAGTGC+AGG 0.646704 6_4:-84875254 None:intergenic
CTTGCTATCTGCCTCCATGT+GGG 0.647974 6_4:+84874385 Msa1001180:five_prime_UTR
TGCTGTTGAGGAAATTTACG+TGG 0.656794 6_4:+84874499 Msa1001180:five_prime_UTR
TGTGGCACGACAAAGGAAGA+GGG 0.660108 6_4:-84871693 None:intergenic
ACACACAATACAAACTAACA+CGG 0.669459 6_4:-84874432 None:intergenic
GATGGTGCATGAAACATCAA+CGG 0.671196 6_4:+84875364 Msa1001180:CDS
TCAGAAAGTTCATACCCACA+TGG 0.705763 6_4:-84874399 None:intergenic
AACTTCGTGGATGAGTGTGA+GGG 0.710468 6_4:-84871824 None:intergenic
GGCACGACAAAGGAAGAGGG+AGG 0.723934 6_4:-84871690 None:intergenic
GAAAGTTCATACCCACATGG+AGG 0.733521 6_4:-84874396 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAATATTATTTAAAATT+AGG - chr6_4:84876255-84876274 None:intergenic 0.0%
!! TTTAAATAATATTTATAATT+AGG + chr6_4:84876258-84876277 Msa1001180:intron 0.0%
!!! AAATATAATCTTTGATTTTT+TGG - chr6_4:84872481-84872500 None:intergenic 10.0%
!!! AAAAATCAAAGATTATATTT+TGG + chr6_4:84872481-84872500 Msa1001180:intron 10.0%
!! TGTTAAAGTTAATATAATAA+CGG + chr6_4:84873776-84873795 Msa1001180:intron 10.0%
!! TATTATATAATGAATGAAAA+TGG + chr6_4:84873970-84873989 Msa1001180:intron 10.0%
!! AAAATAATCAAGTTAATTAT+AGG + chr6_4:84874204-84874223 Msa1001180:intron 10.0%
!!! TAGGTTATATTTTTATTATA+TGG + chr6_4:84874223-84874242 Msa1001180:intron 10.0%
!! AATATATCTGTAAAAATACA+TGG - chr6_4:84872153-84872172 None:intergenic 15.0%
!! TGAAGAAAAAATAAGATAAA+TGG - chr6_4:84872523-84872542 None:intergenic 15.0%
!! TACATATCATAAACAAATAT+TGG - chr6_4:84872567-84872586 None:intergenic 15.0%
!! AAGAAGAAAAAAAATCAATT+TGG - chr6_4:84873636-84873655 None:intergenic 15.0%
!! GTTAAAGTTAATATAATAAC+GGG + chr6_4:84873777-84873796 Msa1001180:intron 15.0%
!! TTAAAGTTAATATAATAACG+GGG + chr6_4:84873778-84873797 Msa1001180:intron 15.0%
!! GTAAGTATAATTTAACAAAA+AGG - chr6_4:84874956-84874975 None:intergenic 15.0%
!! TTGATTACTTAATTATGATT+TGG + chr6_4:84875481-84875500 Msa1001180:intron 15.0%
!! TTAATTTAAAAGTCAACAAA+GGG - chr6_4:84875722-84875741 None:intergenic 15.0%
!!! ATTTAATTTGATTCTTTTCA+TGG - chr6_4:84875873-84875892 None:intergenic 15.0%
!! ATTATATCGTCTTAAATGTA+TGG + chr6_4:84871924-84871943 Msa1001180:intron 20.0%
!!! ATTTACCAAATTCTTTTAGT+AGG - chr6_4:84872059-84872078 None:intergenic 20.0%
!! AAAAAAGGGAATCTAAAAAA+TGG - chr6_4:84872238-84872257 None:intergenic 20.0%
!! GAATAGGTTGTAAAAAAAAA+GGG - chr6_4:84872252-84872271 None:intergenic 20.0%
!! AGAATAGGTTGTAAAAAAAA+AGG - chr6_4:84872253-84872272 None:intergenic 20.0%
!! AAAGGAAAACAAAAAAAAGA+GGG - chr6_4:84872280-84872299 None:intergenic 20.0%
!! TTTATGATATGTATATCTTG+AGG + chr6_4:84872574-84872593 Msa1001180:intron 20.0%
!!! AAGTTAAAGTTTAACTTGTA+TGG + chr6_4:84872782-84872801 Msa1001180:intron 20.0%
!!! TATAACAATTTTGAGATGTA+CGG + chr6_4:84872846-84872865 Msa1001180:intron 20.0%
!!! TAAGCAAATTTTGACTTTTT+AGG - chr6_4:84873017-84873036 None:intergenic 20.0%
!! AACAAAAAATTTGACAGAAT+TGG - chr6_4:84873261-84873280 None:intergenic 20.0%
!! ATCTTTAATGGTAATATGAT+TGG - chr6_4:84873349-84873368 None:intergenic 20.0%
!!! AACTTTTTTTTCTGTGTTTT+TGG + chr6_4:84873498-84873517 Msa1001180:intron 20.0%
!! AAGATATAGAAAACTCAATA+AGG - chr6_4:84873665-84873684 None:intergenic 20.0%
!!! TTCTTTTTTGAAGAAAAAGA+TGG - chr6_4:84874046-84874065 None:intergenic 20.0%
!! TTAGAATAAAGCAAGTTTAA+TGG - chr6_4:84874170-84874189 None:intergenic 20.0%
!! AAAAAACTGAAAATGTGTTT+GGG - chr6_4:84874472-84874491 None:intergenic 20.0%
!! AAAAAAACTGAAAATGTGTT+TGG - chr6_4:84874473-84874492 None:intergenic 20.0%
!!! TACTTTTAAATAACCTTTCT+TGG + chr6_4:84874702-84874721 Msa1001180:five_prime_UTR 20.0%
!!! TTTAAATTTTTGAAGCACAT+AGG - chr6_4:84875007-84875026 None:intergenic 20.0%
!!! TATCATGTATTTTCTTTTGT+TGG + chr6_4:84875137-84875156 Msa1001180:five_prime_UTR 20.0%
!! TTGATATTGTTACTAAATTC+AGG - chr6_4:84875687-84875706 None:intergenic 20.0%
!! GTTAATTTAAAAGTCAACAA+AGG - chr6_4:84875723-84875742 None:intergenic 20.0%
!! GCAAAAGTTCTATTAATTTA+TGG + chr6_4:84875820-84875839 Msa1001180:intron 20.0%
!! TTATTAAGTTATTAGCATAG+TGG + chr6_4:84876184-84876203 Msa1001180:intron 20.0%
!! AGATACAATAAAACAGTAAA+AGG - chr6_4:84876380-84876399 None:intergenic 20.0%
! AAATGATCCGTAGAAAATTT+GGG - chr6_4:84871601-84871620 None:intergenic 25.0%
! GTTTGAATTCGTTATTCTAT+AGG + chr6_4:84871889-84871908 Msa1001180:five_prime_UTR 25.0%
!!! TCTCTAAAATCTAAAACAGA+AGG - chr6_4:84871973-84871992 None:intergenic 25.0%
!!! TCTGTTTTAGATTTTAGAGA+TGG + chr6_4:84871973-84871992 Msa1001180:intron 25.0%
!! TTAGTGTAATTTTGTAGAGA+TGG + chr6_4:84872006-84872025 Msa1001180:intron 25.0%
!!! CCTGTTATAATTTTTTTTGC+CGG + chr6_4:84872031-84872050 Msa1001180:intron 25.0%
! GAAAGGAAAACAAAAAAAAG+AGG - chr6_4:84872281-84872300 None:intergenic 25.0%
!!! TATTTTGGATCTCAACTTAT+CGG + chr6_4:84872496-84872515 Msa1001180:intron 25.0%
!! TTTGATTTAGACCTCTAATT+TGG - chr6_4:84872660-84872679 None:intergenic 25.0%
! ACATTCAAAGAAGGTTATAT+CGG - chr6_4:84872888-84872907 None:intergenic 25.0%
!! AATTTGCTTATAATAGTGAC+CGG - chr6_4:84873027-84873046 None:intergenic 25.0%
! GTCACTATTATAAGCAAATT+TGG + chr6_4:84872918-84872937 Msa1001180:intron 25.0%
! CATTCAATAAATGATGTATG+TGG + chr6_4:84872953-84872972 Msa1001180:intron 25.0%
! CATTCAATAAATGTTGTATG+TGG - chr6_4:84872992-84873011 None:intergenic 25.0%
!! AATTTGCTTATAATAGTGAC+CGG + chr6_4:84873027-84873046 Msa1001180:intron 25.0%
! AAAGTAAAACGAATTGAAGT+GGG - chr6_4:84873085-84873104 None:intergenic 25.0%
! AAAAGTAAAACGAATTGAAG+TGG - chr6_4:84873086-84873105 None:intergenic 25.0%
!!! TTCAATTCGTTTTACTTTTC+AGG + chr6_4:84873087-84873106 Msa1001180:intron 25.0%
!! CATCCTGAATTTACTTATTT+TGG + chr6_4:84873141-84873160 Msa1001180:five_prime_UTR 25.0%
! TCTCCAAAATAAGTAAATTC+AGG - chr6_4:84873147-84873166 None:intergenic 25.0%
! AAAAATTTGACAGAATTGGT+AGG - chr6_4:84873257-84873276 None:intergenic 25.0%
! ATATACGATTCAAGATGATT+GGG - chr6_4:84873534-84873553 None:intergenic 25.0%
! TATATACGATTCAAGATGAT+TGG - chr6_4:84873535-84873554 None:intergenic 25.0%
!!! TATCATCTTTAAAACCCTAT+TGG - chr6_4:84873753-84873772 None:intergenic 25.0%
!! TTAATATAATAACGGGGAAA+GGG + chr6_4:84873784-84873803 Msa1001180:intron 25.0%
!! GAAATTTTGTTCAAATTCAC+TGG + chr6_4:84873821-84873840 Msa1001180:intron 25.0%
!! TAAAAGATCAAACCTTTTAG+TGG + chr6_4:84873868-84873887 Msa1001180:intron 25.0%
!!! CATTGAGACATTTTTGTTAT+AGG + chr6_4:84874008-84874027 Msa1001180:intron 25.0%
!! GAATACAGTTGAATAGTTTT+GGG - chr6_4:84874107-84874126 None:intergenic 25.0%
!!! CATTTTTGAGTTCCATTTAA+GGG - chr6_4:84874361-84874380 None:intergenic 25.0%
!!! TCATTTTTGAGTTCCATTTA+AGG - chr6_4:84874362-84874381 None:intergenic 25.0%
! TACTTGTGTATTCATCTTAA+TGG - chr6_4:84874540-84874559 None:intergenic 25.0%
! ATTGTTCTTACTTACAAATC+AGG - chr6_4:84874578-84874597 None:intergenic 25.0%
! TCATAATAATCAAATGCATC+AGG - chr6_4:84874676-84874695 None:intergenic 25.0%
! TGATGCATTTGATTATTATG+AGG + chr6_4:84874675-84874694 Msa1001180:five_prime_UTR 25.0%
! GTTAAATTATACTTACACCT+TGG + chr6_4:84874960-84874979 Msa1001180:five_prime_UTR 25.0%
!!! TTTTTTATGCAATATGTCCA+AGG - chr6_4:84874980-84874999 None:intergenic 25.0%
! TTCAAACATACTTTATGACT+TGG + chr6_4:84875087-84875106 Msa1001180:five_prime_UTR 25.0%
!! AAAATGTTAATAGAAAGTGC+AGG - chr6_4:84875257-84875276 None:intergenic 25.0%
!!! CTATTTCTAACTGATTCAAA+TGG + chr6_4:84875431-84875450 Msa1001180:CDS 25.0%
! ATTTATGAACTTCTTCACTA+TGG + chr6_4:84875456-84875475 Msa1001180:intron 25.0%
! GATGATTAATTCTATCAGTT+TGG + chr6_4:84875773-84875792 Msa1001180:intron 25.0%
!! GAGAGTTTTTCTTCAAAATT+AGG + chr6_4:84875908-84875927 Msa1001180:intron 25.0%
! GCGAAGAAACATAATTAAAA+TGG - chr6_4:84876097-84876116 None:intergenic 25.0%
! ATAGCTTATTCTATGACAAT+TGG - chr6_4:84876310-84876329 None:intergenic 25.0%
! AACAAACAAACAAAGAAACA+AGG - chr6_4:84876454-84876473 None:intergenic 25.0%
!! TGTTTGTTTGTTAACAGTAT+TGG + chr6_4:84876470-84876489 Msa1001180:intron 25.0%
! ATTTAGAAATTCGGTAGAAA+AGG - chr6_4:84876580-84876599 None:intergenic 25.0%
GAAATGATCCGTAGAAAATT+TGG - chr6_4:84871602-84871621 None:intergenic 30.0%
!! AAGTTTCTCATTGACTTACT+GGG - chr6_4:84871634-84871653 None:intergenic 30.0%
!! AAAGTTTCTCATTGACTTAC+TGG - chr6_4:84871635-84871654 None:intergenic 30.0%
AATTCGTTATTCTATAGGCA+CGG + chr6_4:84871894-84871913 Msa1001180:five_prime_UTR 30.0%
!! GATTTTAGAGATGGTTTTTG+TGG + chr6_4:84871982-84872001 Msa1001180:intron 30.0%
CCGGCAAAAAAAATTATAAC+AGG - chr6_4:84872034-84872053 None:intergenic 30.0%
! CAAATTCTTTTAGTAGGAAC+CGG - chr6_4:84872053-84872072 None:intergenic 30.0%
ATTGTGTAAGTAGGTCAAAA+GGG - chr6_4:84872396-84872415 None:intergenic 30.0%
GTTACAAAGATTGTGTAAGT+AGG - chr6_4:84872405-84872424 None:intergenic 30.0%
! CTCTAATTTGGTTTATCAAG+TGG - chr6_4:84872648-84872667 None:intergenic 30.0%
CACTTGATAAACCAAATTAG+AGG + chr6_4:84872646-84872665 Msa1001180:intron 30.0%
! AACTTTGCTTGCTAATATCT+TGG + chr6_4:84872755-84872774 Msa1001180:intron 30.0%
! ACTTTGCTTGCTAATATCTT+GGG + chr6_4:84872756-84872775 Msa1001180:intron 30.0%
GATTACCACTTACAAAATCA+AGG + chr6_4:84873165-84873184 Msa1001180:five_prime_UTR 30.0%
! GATTACCTTGATTTTGTAAG+TGG - chr6_4:84873173-84873192 None:intergenic 30.0%
AACGAGTAAATATGGTATGT+TGG - chr6_4:84873204-84873223 None:intergenic 30.0%
GCATTGAAAACGAGTAAATA+TGG - chr6_4:84873212-84873231 None:intergenic 30.0%
! TTTACTCGTTTTCAATGCAT+AGG + chr6_4:84873214-84873233 Msa1001180:intron 30.0%
CATAGGATAATGCAAAAAAG+AGG + chr6_4:84873231-84873250 Msa1001180:intron 30.0%
! ACTTGCGTTTTCATCTTTAA+TGG - chr6_4:84873361-84873380 None:intergenic 30.0%
TTTCATATTTGCTCACGAAT+AGG + chr6_4:84873384-84873403 Msa1001180:intron 30.0%
TTCATATTTGCTCACGAATA+GGG + chr6_4:84873385-84873404 Msa1001180:intron 30.0%
!! ATCTAAACTTGTTAAAGTGC+AGG + chr6_4:84873459-84873478 Msa1001180:intron 30.0%
!! TCTAAACTTGTTAAAGTGCA+GGG + chr6_4:84873460-84873479 Msa1001180:intron 30.0%
! ACATTTTTCCTTCAACCAAT+AGG + chr6_4:84873735-84873754 Msa1001180:intron 30.0%
! CATTTTTCCTTCAACCAATA+GGG + chr6_4:84873736-84873755 Msa1001180:intron 30.0%
! TTTAAAACCCTATTGGTTGA+AGG - chr6_4:84873746-84873765 None:intergenic 30.0%
! GTTAATATAATAACGGGGAA+AGG + chr6_4:84873783-84873802 Msa1001180:intron 30.0%
! GGAATACAGTTGAATAGTTT+TGG - chr6_4:84874108-84874127 None:intergenic 30.0%
ATTCAGTCAAAAATGTTTGC+AGG - chr6_4:84874129-84874148 None:intergenic 30.0%
ACACACAATACAAACTAACA+CGG - chr6_4:84874435-84874454 None:intergenic 30.0%
!!! TTTTTTCTTGTTTGCTGTTG+AGG + chr6_4:84874487-84874506 Msa1001180:five_prime_UTR 30.0%
TTACAAATCAGGATCAAAAG+TGG - chr6_4:84874567-84874586 None:intergenic 30.0%
! AATAACCTTTCTTGGACTTT+TGG + chr6_4:84874710-84874729 Msa1001180:five_prime_UTR 30.0%
GTAAATCAACGATTCTTGAA+TGG + chr6_4:84874739-84874758 Msa1001180:five_prime_UTR 30.0%
! GTGTAGAAAAGTTGCATTTT+TGG + chr6_4:84874810-84874829 Msa1001180:five_prime_UTR 30.0%
!! CATTTTTGGATATAACTTGC+TGG + chr6_4:84874824-84874843 Msa1001180:five_prime_UTR 30.0%
! CATGTATTTTCTTTTGTTGG+TGG + chr6_4:84875140-84875159 Msa1001180:five_prime_UTR 30.0%
TGGTGGTAAATAAGTCTAAA+TGG + chr6_4:84875157-84875176 Msa1001180:five_prime_UTR 30.0%
! CTGATAACTTTGTTTCTTCT+TGG + chr6_4:84875288-84875307 Msa1001180:five_prime_UTR 30.0%
!! ACTTTGTTTCTTCTTGGTTT+TGG + chr6_4:84875294-84875313 Msa1001180:five_prime_UTR 30.0%
!!! TTTCTTCTTGGTTTTGGATT+GGG + chr6_4:84875300-84875319 Msa1001180:five_prime_UTR 30.0%
TCTTCGCTAATCAATTTACA+CGG + chr6_4:84876110-84876129 Msa1001180:intron 30.0%
! ATGACAATTTTTGTATCAGC+TGG + chr6_4:84876208-84876227 Msa1001180:intron 30.0%
TTCTATGACAATTGGCATAA+GGG - chr6_4:84876302-84876321 None:intergenic 30.0%
ATTCTATGACAATTGGCATA+AGG - chr6_4:84876303-84876322 None:intergenic 30.0%
GTACATATCAAGCTTGATAA+AGG - chr6_4:84876333-84876352 None:intergenic 30.0%
! TTTGTTAACAGTATTGGATG+TGG + chr6_4:84876476-84876495 Msa1001180:intron 30.0%
GTAGAAAAGGAAAACTTACA+TGG - chr6_4:84876567-84876586 None:intergenic 30.0%
CGGTTCCTACTAAAAGAATT+TGG + chr6_4:84872051-84872070 Msa1001180:intron 35.0%
AAAAAAGAGGGAGAGAGAAT+AGG - chr6_4:84872268-84872287 None:intergenic 35.0%
! TCCGCAAAAAGCTTCATTTT+CGG + chr6_4:84872310-84872329 Msa1001180:five_prime_UTR 35.0%
! ACCGAAAATGAAGCTTTTTG+CGG - chr6_4:84872314-84872333 None:intergenic 35.0%
AAAGGGAACTTACATGATGA+AGG - chr6_4:84872379-84872398 None:intergenic 35.0%
GATTGTGTAAGTAGGTCAAA+AGG - chr6_4:84872397-84872416 None:intergenic 35.0%
TCATATTTGCTCACGAATAG+GGG + chr6_4:84873386-84873405 Msa1001180:intron 35.0%
TTTGTGCACACTCTTTAGAT+TGG + chr6_4:84873436-84873455 Msa1001180:intron 35.0%
AGTGCAGGGTAACTTAAAAA+TGG + chr6_4:84873474-84873493 Msa1001180:intron 35.0%
! CTGCTTAGTTCTCTAGAAAT+AGG + chr6_4:84873891-84873910 Msa1001180:intron 35.0%
!!! GTTGAATAGTTTTGGGTGAA+AGG - chr6_4:84874100-84874119 None:intergenic 35.0%
TGTATTCTTGCTTATGATGC+AGG + chr6_4:84874288-84874307 Msa1001180:intron 35.0%
GTATTCTTGCTTATGATGCA+GGG + chr6_4:84874289-84874308 Msa1001180:intron 35.0%
AAACAGAAGTCACCCTTAAA+TGG + chr6_4:84874346-84874365 Msa1001180:five_prime_UTR 35.0%
ATACAAACTAACACGGATGA+AGG - chr6_4:84874428-84874447 None:intergenic 35.0%
GCATCAGGTGTATGTTTAAA+AGG - chr6_4:84874661-84874680 None:intergenic 35.0%
!!! GTTTCTTCTTGGTTTTGGAT+TGG + chr6_4:84875299-84875318 Msa1001180:five_prime_UTR 35.0%
!!! TTCTTCTTGGTTTTGGATTG+GGG + chr6_4:84875301-84875320 Msa1001180:five_prime_UTR 35.0%
! CTATGAAGTTTTTCGTCGAA+TGG + chr6_4:84875388-84875407 Msa1001180:CDS 35.0%
ATCAGTTTGGAAATATCTGC+TGG + chr6_4:84875786-84875805 Msa1001180:intron 35.0%
TCAGTTTGGAAATATCTGCT+GGG + chr6_4:84875787-84875806 Msa1001180:intron 35.0%
TGGTAGTTTAGCCTCTTAAA+AGG + chr6_4:84875840-84875859 Msa1001180:intron 35.0%
!!! ATGGAAGCATTCCTTTTAAG+AGG - chr6_4:84875854-84875873 None:intergenic 35.0%
AACGTACATTAGGTGAATCT+AGG - chr6_4:84876066-84876085 None:intergenic 35.0%
CACATCTATTCGAAATGCTT+GGG - chr6_4:84876150-84876169 None:intergenic 35.0%
TCACATCTATTCGAAATGCT+TGG - chr6_4:84876151-84876170 None:intergenic 35.0%
AGCATTTCGAATAGATGTGA+TGG + chr6_4:84876151-84876170 Msa1001180:intron 35.0%
GCATTTCGAATAGATGTGAT+GGG + chr6_4:84876152-84876171 Msa1001180:intron 35.0%
! AATAGATGTGATGGGACAAA+AGG + chr6_4:84876160-84876179 Msa1001180:intron 35.0%
TATTGGATGTGGTTGAGTTT+GGG + chr6_4:84876487-84876506 Msa1001180:CDS 35.0%
! GACGATGACTTTTTACAGTG+TGG - chr6_4:84871714-84871733 None:intergenic 40.0%
CAAATTCGTGTCAAAGGCAA+AGG - chr6_4:84871765-84871784 None:intergenic 40.0%
GGACATCAAATTCGTGTCAA+AGG - chr6_4:84871771-84871790 None:intergenic 40.0%
!! AAACACTGACTTCGTTGTTG+TGG - chr6_4:84871873-84871892 None:intergenic 40.0%
TTCTTTGAATGTACTCCCTC+CGG + chr6_4:84872896-84872915 Msa1001180:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr6_4:84873031-84873050 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr6_4:84873030-84873049 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr6_4:84873030-84873049 Msa1001180:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr6_4:84873031-84873050 Msa1001180:intron 40.0%
ATAACAAACCATACTCCCTC+CGG - chr6_4:84873049-84873068 None:intergenic 40.0%
CATATTTGCTCACGAATAGG+GGG + chr6_4:84873387-84873406 Msa1001180:intron 40.0%
ATATTTGCTCACGAATAGGG+GGG + chr6_4:84873388-84873407 Msa1001180:intron 40.0%
GTGCACAAAAGAAACTGTTC+AGG - chr6_4:84873425-84873444 None:intergenic 40.0%
TCAAGATGATTGGGATGGTT+GGG - chr6_4:84873525-84873544 None:intergenic 40.0%
TTCAAGATGATTGGGATGGT+TGG - chr6_4:84873526-84873545 None:intergenic 40.0%
ACGATTCAAGATGATTGGGA+TGG - chr6_4:84873530-84873549 None:intergenic 40.0%
AGAACTAAGCAGCCACTAAA+AGG - chr6_4:84873883-84873902 None:intergenic 40.0%
! AGGGTGACTTCTGTTTTCTA+AGG - chr6_4:84874342-84874361 None:intergenic 40.0%
TCAGAAAGTTCATACCCACA+TGG - chr6_4:84874402-84874421 None:intergenic 40.0%
TGCTGTTGAGGAAATTTACG+TGG + chr6_4:84874499-84874518 Msa1001180:five_prime_UTR 40.0%
ACACACCAAAAGTCCAAGAA+AGG - chr6_4:84874718-84874737 None:intergenic 40.0%
!! TTGGATATAACTTGCTGGTG+TGG + chr6_4:84874829-84874848 Msa1001180:five_prime_UTR 40.0%
!! TGGATATAACTTGCTGGTGT+GGG + chr6_4:84874830-84874849 Msa1001180:five_prime_UTR 40.0%
! TTATGACTTGGCTGTACATG+AGG + chr6_4:84875099-84875118 Msa1001180:five_prime_UTR 40.0%
!!! TCTTCTTGGTTTTGGATTGG+GGG + chr6_4:84875302-84875321 Msa1001180:five_prime_UTR 40.0%
TTGAAAGTCGCAATGATGTC+AGG - chr6_4:84875337-84875356 None:intergenic 40.0%
GACATCATTGCGACTTTCAA+AGG + chr6_4:84875337-84875356 Msa1001180:CDS 40.0%
GATGGTGCATGAAACATCAA+CGG + chr6_4:84875364-84875383 Msa1001180:CDS 40.0%
ATCATAGATCGTCGATGTCT+TGG + chr6_4:84875528-84875547 Msa1001180:intron 40.0%
TCATAGATCGTCGATGTCTT+GGG + chr6_4:84875529-84875548 Msa1001180:intron 40.0%
CTCTTATGCGTTCAACTGAT+TGG + chr6_4:84875958-84875977 Msa1001180:intron 40.0%
CATTAGGTGAATCTAGGTGA+GGG - chr6_4:84876060-84876079 None:intergenic 40.0%
ACATTAGGTGAATCTAGGTG+AGG - chr6_4:84876061-84876080 None:intergenic 40.0%
GTATTGGATGTGGTTGAGTT+TGG + chr6_4:84876486-84876505 Msa1001180:intron 40.0%
ATTGGATGTGGTTGAGTTTG+GGG + chr6_4:84876488-84876507 Msa1001180:CDS 40.0%
!! CTCATTGACTTACTGGGATG+TGG - chr6_4:84871628-84871647 None:intergenic 45.0%
TTTACAGTGTGGCACGACAA+AGG - chr6_4:84871703-84871722 None:intergenic 45.0%
! AAAGGACGAGATTGGGGTTT+TGG - chr6_4:84871747-84871766 None:intergenic 45.0%
AAAGGCAAAGGACGAGATTG+GGG - chr6_4:84871753-84871772 None:intergenic 45.0%
CAAAGGCAAAGGACGAGATT+GGG - chr6_4:84871754-84871773 None:intergenic 45.0%
TCAAAGGCAAAGGACGAGAT+TGG - chr6_4:84871755-84871774 None:intergenic 45.0%
! GGAAACAAAGCTTTTGGTGG+CGG - chr6_4:84871799-84871818 None:intergenic 45.0%
!! GCCACCAAAAGCTTTGTTTC+CGG + chr6_4:84871798-84871817 Msa1001180:five_prime_UTR 45.0%
! ACCGGAAACAAAGCTTTTGG+TGG - chr6_4:84871802-84871821 None:intergenic 45.0%
! GTGACCGGAAACAAAGCTTT+TGG - chr6_4:84871805-84871824 None:intergenic 45.0%
AACTTCGTGGATGAGTGTGA+GGG - chr6_4:84871827-84871846 None:intergenic 45.0%
TAACTTCGTGGATGAGTGTG+AGG - chr6_4:84871828-84871847 None:intergenic 45.0%
TTTGCGGAGTCGAATCTGAA+AGG - chr6_4:84872298-84872317 None:intergenic 45.0%
! GTCACTTTCAAGCTCAACCA+TGG + chr6_4:84872332-84872351 Msa1001180:five_prime_UTR 45.0%
AGCTCAACCATGGAGTTTAG+CGG + chr6_4:84872342-84872361 Msa1001180:five_prime_UTR 45.0%
GTGGATGAGGATAAGTCACA+CGG - chr6_4:84872431-84872450 None:intergenic 45.0%
GTGACTTATCCTCATCCACA+AGG + chr6_4:84872432-84872451 Msa1001180:intron 45.0%
TTGCATGTGTGAGATCCTTG+TGG - chr6_4:84872450-84872469 None:intergenic 45.0%
! GGAGGGAGTACATTCAAAGA+AGG - chr6_4:84872897-84872916 None:intergenic 45.0%
TATTTGCTCACGAATAGGGG+GGG + chr6_4:84873389-84873408 Msa1001180:intron 45.0%
TTCCTGTCCTACTCCTTTCT+GGG + chr6_4:84873914-84873933 Msa1001180:intron 45.0%
CAACAAACCCAGAAAGGAGT+AGG - chr6_4:84873924-84873943 None:intergenic 45.0%
!! ACTCCTTTCTGGGTTTGTTG+TGG + chr6_4:84873924-84873943 Msa1001180:intron 45.0%
!! CTCCTTTCTGGGTTTGTTGT+GGG + chr6_4:84873925-84873944 Msa1001180:intron 45.0%
TTCTTGCTTATGATGCAGGG+AGG + chr6_4:84874292-84874311 Msa1001180:intron 45.0%
GAAAGTTCATACCCACATGG+AGG - chr6_4:84874399-84874418 None:intergenic 45.0%
!!! CTTCTTGGTTTTGGATTGGG+GGG + chr6_4:84875303-84875322 Msa1001180:five_prime_UTR 45.0%
!!! TTCTTGGTTTTGGATTGGGG+GGG + chr6_4:84875304-84875323 Msa1001180:five_prime_UTR 45.0%
!! GCGACTTTCAAAGGCATTGA+TGG + chr6_4:84875346-84875365 Msa1001180:CDS 45.0%
! TTTCCAGTGCATTGACCATG+AGG + chr6_4:84876008-84876027 Msa1001180:intron 45.0%
! TTCCAGTGCATTGACCATGA+GGG + chr6_4:84876009-84876028 Msa1001180:intron 45.0%
CTAGAAGCAAGAGTTCTGCA+AGG + chr6_4:84876516-84876535 Msa1001180:CDS 45.0%
TCTCCCCTCGAAAACCCTAA+CGG + chr6_4:84871661-84871680 Msa1001180:five_prime_UTR 50.0%
TGTGGCACGACAAAGGAAGA+GGG - chr6_4:84871696-84871715 None:intergenic 50.0%
!!! AAGCTTTTGGTGGCGGAGAT+CGG - chr6_4:84871792-84871811 None:intergenic 50.0%
CAGATCTGCTGCTAACTTCG+TGG - chr6_4:84871840-84871859 None:intergenic 50.0%
AGATCTGCCGCTAAACTCCA+TGG - chr6_4:84872352-84872371 None:intergenic 50.0%
GTGTGAGATCCTTGTGGATG+AGG - chr6_4:84872444-84872463 None:intergenic 50.0%
AATAGTGACCGGAGGGAGTA+TGG + chr6_4:84873038-84873057 Msa1001180:intron 50.0%
ATTTGCTCACGAATAGGGGG+GGG + chr6_4:84873390-84873409 Msa1001180:intron 50.0%
GTTCCTGTCCTACTCCTTTC+TGG + chr6_4:84873913-84873932 Msa1001180:intron 50.0%
AACCCAGAAAGGAGTAGGAC+AGG - chr6_4:84873919-84873938 None:intergenic 50.0%
TGCCCACAACAAACCCAGAA+AGG - chr6_4:84873930-84873949 None:intergenic 50.0%
! CTTGCTATCTGCCTCCATGT+GGG + chr6_4:84874385-84874404 Msa1001180:five_prime_UTR 50.0%
TCTGTCTCTCATCACCCTCA+TGG - chr6_4:84876026-84876045 None:intergenic 50.0%
GTGAATCTAGGTGAGGGAAG+TGG - chr6_4:84876054-84876073 None:intergenic 50.0%
!! CTGCCGTTAGGGTTTTCGAG+GGG - chr6_4:84871667-84871686 None:intergenic 55.0%
! GCTGCCGTTAGGGTTTTCGA+GGG - chr6_4:84871668-84871687 None:intergenic 55.0%
ACGACAAAGGAAGAGGGAGG+TGG - chr6_4:84871690-84871709 None:intergenic 55.0%
GTGTGGCACGACAAAGGAAG+AGG - chr6_4:84871697-84871716 None:intergenic 55.0%
TGGATGAGTGTGAGGGTGAC+CGG - chr6_4:84871820-84871839 None:intergenic 55.0%
TTTGCTCACGAATAGGGGGG+GGG + chr6_4:84873391-84873410 Msa1001180:intron 55.0%
!! GCTTGCTATCTGCCTCCATG+TGG + chr6_4:84874384-84874403 Msa1001180:five_prime_UTR 55.0%
CACCCTCATGGTCAATGCAC+TGG - chr6_4:84876014-84876033 None:intergenic 55.0%
! GGCTGCCGTTAGGGTTTTCG+AGG - chr6_4:84871669-84871688 None:intergenic 60.0%
GGCACGACAAAGGAAGAGGG+AGG - chr6_4:84871693-84871712 None:intergenic 60.0%
GAGGGAGGTGGCTGCCGTTA+GGG - chr6_4:84871678-84871697 None:intergenic 65.0%
AGAGGGAGGTGGCTGCCGTT+AGG - chr6_4:84871679-84871698 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 84871592 84876605 84871592 ID=Msa1001180;Name=Msa1001180
chr6_4 mRNA 84871592 84876605 84871592 ID=Msa1001180-mRNA-1;Parent=Msa1001180;Name=Msa1001180-mRNA-1;_AED=0.14;_eAED=0.14;_QI=1459|0.8|1|1|0.2|0.16|6|54|62
chr6_4 exon 84871592 84871915 84871592 ID=Msa1001180-mRNA-1:exon:16556;Parent=Msa1001180-mRNA-1
chr6_4 exon 84872303 84872385 84872303 ID=Msa1001180-mRNA-1:exon:16557;Parent=Msa1001180-mRNA-1
chr6_4 exon 84873109 84873186 84873109 ID=Msa1001180-mRNA-1:exon:16558;Parent=Msa1001180-mRNA-1
chr6_4 exon 84874310 84875040 84874310 ID=Msa1001180-mRNA-1:exon:16559;Parent=Msa1001180-mRNA-1
chr6_4 exon 84875086 84875452 84875086 ID=Msa1001180-mRNA-1:exon:16560;Parent=Msa1001180-mRNA-1
chr6_4 exon 84876487 84876605 84876487 ID=Msa1001180-mRNA-1:exon:16561;Parent=Msa1001180-mRNA-1
chr6_4 five_prime_UTR 84871592 84871915 84871592 ID=Msa1001180-mRNA-1:five_prime_utr;Parent=Msa1001180-mRNA-1
chr6_4 five_prime_UTR 84872303 84872385 84872303 ID=Msa1001180-mRNA-1:five_prime_utr;Parent=Msa1001180-mRNA-1
chr6_4 five_prime_UTR 84873109 84873186 84873109 ID=Msa1001180-mRNA-1:five_prime_utr;Parent=Msa1001180-mRNA-1
chr6_4 five_prime_UTR 84874310 84875040 84874310 ID=Msa1001180-mRNA-1:five_prime_utr;Parent=Msa1001180-mRNA-1
chr6_4 five_prime_UTR 84875086 84875328 84875086 ID=Msa1001180-mRNA-1:five_prime_utr;Parent=Msa1001180-mRNA-1
chr6_4 CDS 84875329 84875452 84875329 ID=Msa1001180-mRNA-1:cds;Parent=Msa1001180-mRNA-1
chr6_4 CDS 84876487 84876551 84876487 ID=Msa1001180-mRNA-1:cds;Parent=Msa1001180-mRNA-1
chr6_4 three_prime_UTR 84876552 84876605 84876552 ID=Msa1001180-mRNA-1:three_prime_utr;Parent=Msa1001180-mRNA-1
Gene Sequence

>Msa1001180

ATGAACCTGACATCATTGCGACTTTCAAAGGCATTGATGGTGCATGAAACATCAACGGTCTATGAAGTTTTTCGTCGAATGGCTATTTGCAGAGTAGATGCTCTATTTCTAACTGATTCAAATGTATTGGATGTGGTTGAGTTTGGGGAGCTGCTAGAAGCAAGAGTTCTGCAAGGAGTCAATAGTTAG
Protein sequence

>Msa1001180

MNLTSLRLSKALMVHETSTVYEVFRRMAICRVDALFLTDSNVLDVVEFGELLEARVLQGVNS*