AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0335960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0335960 MtrunA17_Chr2g0321501 96.129 155 6 0 6 160 1 155 6.57e-110 309
Msa0335960 MtrunA17_Chr4g0026331 73.548 155 41 0 6 160 1 155 2.89e-83 242
Msa0335960 MtrunA17_Chr1g0210631 63.750 160 52 2 1 160 65 218 3.30e-66 201
Msa0335960 MtrunA17_Chr1g0210661 71.756 131 36 1 29 159 24 153 3.07e-64 193
Msa0335960 MtrunA17_Chr8g0380641 60.976 123 46 2 38 159 25 146 1.92e-48 153
Msa0335960 MtrunA17_Chr2g0277481 59.504 121 45 2 42 159 29 148 9.02e-48 152
Msa0335960 MtrunA17_Chr5g0394481 40.404 99 57 2 61 159 47 143 1.89e-21 84.7
Msa0335960 MtrunA17_Chr4g0054691 28.571 147 100 3 18 160 14 159 3.47e-16 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0335960 AT2G27510.1 60.870 161 56 3 1 160 1 155 3.51e-64 194
Msa0335960 AT1G60950.1 66.667 117 36 2 45 159 32 147 2.64e-50 158
Msa0335960 AT1G10960.1 67.544 114 34 2 45 156 32 144 1.39e-47 151
Msa0335960 AT5G10000.1 57.143 112 46 1 50 159 36 147 7.57e-44 142
Msa0335960 AT4G14890.1 45.263 95 50 2 65 159 52 144 3.87e-22 87.0
Msa0335960 AT1G32550.1 39.535 86 52 0 75 160 69 154 7.35e-18 76.6
Msa0335960 AT1G32550.2 37.255 102 45 3 75 160 69 167 3.24e-13 64.7

Find 38 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGCTGGGAAGGTTGTTTC+TGG 0.160515 2_4:+55231363 Msa0335960:CDS
TCTCGATGACAATCAATTAA+AGG 0.218310 2_4:+55231413 Msa0335960:CDS
CGGCAGTAGTGAATTGATTT+TGG 0.237516 2_4:-55231104 None:intergenic
AAGAATGTTTCACGATCTTT+TGG 0.297059 2_4:+55231162 Msa0335960:CDS
GGAGCATGCTCTACCTGTGC+TGG 0.371691 2_4:+55231348 Msa0335960:CDS
TAGATGAAGCAGATGACTTC+AGG 0.402843 2_4:-55231185 None:intergenic
CAAAATTAAGCTGATTGGTC+CGG 0.411846 2_4:+55231230 Msa0335960:CDS
TGAAGTCATCTGCTTCATCT+AGG 0.420369 2_4:+55231187 Msa0335960:CDS
GCTTACAAAATTAAGCTGAT+TGG 0.422819 2_4:+55231225 Msa0335960:CDS
CTTCCGTATTCATGCCGTGC+TGG 0.464676 2_4:+55231327 Msa0335960:CDS
TGAATAACACAGTCTGAAGT+TGG 0.477269 2_4:-55231465 None:intergenic
TAAGCATAACAAACCAGGTC+TGG 0.479662 2_4:-55231580 None:intergenic
GCTCCAGCACGGCATGAATA+CGG 0.486288 2_4:-55231330 None:intergenic
AGATAATGTGCGCTGTTGCT+TGG 0.490262 2_4:+55231681 Msa0335960:three_prime_UTR
GTCAAGTGCCCTTCCACTCT+AGG 0.511274 2_4:+55231132 Msa0335960:CDS
ATGCTCTACCTGTGCTGGGA+AGG 0.518844 2_4:+55231353 Msa0335960:CDS
GAGCATGCTCTACCTGTGCT+GGG 0.533531 2_4:+55231349 Msa0335960:CDS
GAAGTCATCTGCTTCATCTA+GGG 0.543475 2_4:+55231188 Msa0335960:CDS
GTCAGCCTTGTCAACTGTGA+AGG 0.544738 2_4:+55231050 Msa0335960:CDS
TTCGCAGATCCTAGAGTGGA+AGG 0.547384 2_4:-55231141 None:intergenic
TTCATCTAGGGTTACTGCAG+TGG 0.553859 2_4:+55231200 Msa0335960:CDS
GGTTCTGTCGATCAGTCAGA+TGG 0.558348 2_4:+55231384 Msa0335960:CDS
GTTATTCATACGCACAAAGA+AGG 0.560127 2_4:+55231480 Msa0335960:CDS
ATTAAGCTGATTGGTCCGGA+TGG 0.560404 2_4:+55231234 Msa0335960:CDS
AGAAACAACCTTCCCAGCAC+AGG 0.578473 2_4:-55231361 None:intergenic
TTAGATGCAGCAGAGAATGC+CGG 0.589828 2_4:+55231297 Msa0335960:CDS
GATGACAATCAATTAAAGGA+AGG 0.602977 2_4:+55231417 Msa0335960:CDS
TTAGACATTACAGCCAGACC+TGG 0.611763 2_4:+55231567 Msa0335960:three_prime_UTR
GGAAGGGCACTTGACTGTTG+CGG 0.612607 2_4:-55231124 None:intergenic
AGGTAGAGCATGCTCCAGCA+CGG 0.616285 2_4:-55231341 None:intergenic
CATACAGAAAATCATCGAAG+AGG 0.617843 2_4:-55231723 None:intergenic
TGAAGTTGGATAAGCTACAC+AGG 0.630428 2_4:-55231451 None:intergenic
AAAACCTGTGCCCTTGTTGG+GGG 0.644764 2_4:+55231528 Msa0335960:three_prime_UTR
TGAATACGGAAGTTCAACTC+CGG 0.648667 2_4:-55231316 None:intergenic
GTAAACCTTCACAGTTGACA+AGG 0.652422 2_4:-55231055 None:intergenic
TCGCAGATCCTAGAGTGGAA+GGG 0.653095 2_4:-55231140 None:intergenic
ACATATAAGCATAACAAACC+AGG 0.675316 2_4:-55231585 None:intergenic
ATTCTTCGCAGATCCTAGAG+TGG 0.678656 2_4:-55231145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTCAACTTTTTTAAAT+TGG + chr2_4:55229672-55229691 Msa0335960:intron 10.0%
!! AATAAATTATCTAATGATAT+TGG + chr2_4:55230061-55230080 Msa0335960:intron 10.0%
!!! AATATTCAACTTTTTTAAAT+TGG + chr2_4:55229672-55229691 Msa0335960:intron 10.0%
!! AATAAATTATCTAATGATAT+TGG + chr2_4:55230061-55230080 Msa0335960:intron 10.0%
!!! ATAAGGTATTTATATAACAA+AGG - chr2_4:55230288-55230307 None:intergenic 15.0%
!! ATTAAGTTCTTTATTCAAAT+AGG + chr2_4:55230353-55230372 Msa0335960:intron 15.0%
!!! AAATAAACTGTTTAACATTT+TGG + chr2_4:55230733-55230752 Msa0335960:intron 15.0%
!! AATTGAATTGATATTACAAA+TGG - chr2_4:55231017-55231036 None:intergenic 15.0%
!!! ATAAGGTATTTATATAACAA+AGG - chr2_4:55230288-55230307 None:intergenic 15.0%
!! ATTAAGTTCTTTATTCAAAT+AGG + chr2_4:55230353-55230372 Msa0335960:intron 15.0%
!!! AAATAAACTGTTTAACATTT+TGG + chr2_4:55230733-55230752 Msa0335960:intron 15.0%
!! AATTGAATTGATATTACAAA+TGG - chr2_4:55231017-55231036 None:intergenic 15.0%
!! TATATAACAACGATATGATA+TGG - chr2_4:55229500-55229519 None:intergenic 20.0%
!! GATGCAAAAAAAAAACTATA+GGG - chr2_4:55229527-55229546 None:intergenic 20.0%
!!! ACTTTTTTAAATTGGATAAG+AGG + chr2_4:55229680-55229699 Msa0335960:intron 20.0%
!!! TACATTCATAGTTTAATTTC+AGG + chr2_4:55229818-55229837 Msa0335960:intron 20.0%
!!! AAACTATAAGTTGTTTTCAT+CGG - chr2_4:55230243-55230262 None:intergenic 20.0%
!! TTGTCTATGTTAAAGATAAA+AGG - chr2_4:55230395-55230414 None:intergenic 20.0%
!! TGTAATATCAATTCAATTTC+AGG + chr2_4:55231019-55231038 Msa0335960:intron 20.0%
!! GTAATATCAATTCAATTTCA+GGG + chr2_4:55231020-55231039 Msa0335960:intron 20.0%
!!! TTTTAAGTTATGTACGATAA+TGG + chr2_4:55231611-55231630 Msa0335960:three_prime_UTR 20.0%
!! TATATAACAACGATATGATA+TGG - chr2_4:55229500-55229519 None:intergenic 20.0%
!! GATGCAAAAAAAAAACTATA+GGG - chr2_4:55229527-55229546 None:intergenic 20.0%
!!! ACTTTTTTAAATTGGATAAG+AGG + chr2_4:55229680-55229699 Msa0335960:intron 20.0%
!!! TACATTCATAGTTTAATTTC+AGG + chr2_4:55229818-55229837 Msa0335960:intron 20.0%
!!! AAACTATAAGTTGTTTTCAT+CGG - chr2_4:55230243-55230262 None:intergenic 20.0%
!! TTGTCTATGTTAAAGATAAA+AGG - chr2_4:55230395-55230414 None:intergenic 20.0%
!! TGTAATATCAATTCAATTTC+AGG + chr2_4:55231019-55231038 Msa0335960:intron 20.0%
!! GTAATATCAATTCAATTTCA+GGG + chr2_4:55231020-55231039 Msa0335960:intron 20.0%
!!! TTTTAAGTTATGTACGATAA+TGG + chr2_4:55231611-55231630 Msa0335960:three_prime_UTR 20.0%
! CGATGCAAAAAAAAAACTAT+AGG - chr2_4:55229528-55229547 None:intergenic 25.0%
! CTAAGAATTAAGGATTCATT+TGG + chr2_4:55229578-55229597 Msa0335960:intron 25.0%
! ACTAATAAAATGCATCTGTA+AGG + chr2_4:55229890-55229909 Msa0335960:intron 25.0%
!! AATTCATTTTCTTTTCCATC+CGG - chr2_4:55231252-55231271 None:intergenic 25.0%
! CGATGCAAAAAAAAAACTAT+AGG - chr2_4:55229528-55229547 None:intergenic 25.0%
! CTAAGAATTAAGGATTCATT+TGG + chr2_4:55229578-55229597 Msa0335960:intron 25.0%
! ACTAATAAAATGCATCTGTA+AGG + chr2_4:55229890-55229909 Msa0335960:intron 25.0%
!! AATTCATTTTCTTTTCCATC+CGG - chr2_4:55231252-55231271 None:intergenic 25.0%
! ATAGATTATCTCCTCCTTTT+TGG - chr2_4:55229367-55229386 None:intergenic 30.0%
! GTAATGGAAAAACTTACTGT+TGG - chr2_4:55229478-55229497 None:intergenic 30.0%
ACAACGATATGATATGGTAA+TGG - chr2_4:55229494-55229513 None:intergenic 30.0%
!! ACTATCTGCTCTAAGAATTA+AGG + chr2_4:55229568-55229587 Msa0335960:intron 30.0%
ATGTTTATGATGTCTTAGCT+CGG - chr2_4:55229799-55229818 None:intergenic 30.0%
! CAGATGCATTTTATTAGTGT+TGG - chr2_4:55229888-55229907 None:intergenic 30.0%
CTCACAAATATTTATGTCAC+TGG - chr2_4:55230138-55230157 None:intergenic 30.0%
AGACTACTTCGAAAAGTTTA+TGG + chr2_4:55230156-55230175 Msa0335960:intron 30.0%
TATAGTTCAGTTCTTTGGTT+GGG + chr2_4:55230444-55230463 Msa0335960:intron 30.0%
AATTCGATGTCTAACTAAGA+GGG - chr2_4:55230544-55230563 None:intergenic 30.0%
TCTTAGTTAGACATCGAATT+CGG + chr2_4:55230544-55230563 Msa0335960:intron 30.0%
! TCATTTATTCCTTTTGTGTG+TGG + chr2_4:55230659-55230678 Msa0335960:intron 30.0%
ATCTTATACAAAACACAGCA+GGG - chr2_4:55230993-55231012 None:intergenic 30.0%
! AAGAATGTTTCACGATCTTT+TGG + chr2_4:55231162-55231181 Msa0335960:CDS 30.0%
GCTTACAAAATTAAGCTGAT+TGG + chr2_4:55231225-55231244 Msa0335960:CDS 30.0%
TCTCGATGACAATCAATTAA+AGG + chr2_4:55231413-55231432 Msa0335960:CDS 30.0%
GATGACAATCAATTAAAGGA+AGG + chr2_4:55231417-55231436 Msa0335960:CDS 30.0%
! TTGGGAGAAATTTTCAATAG+AGG - chr2_4:55231509-55231528 None:intergenic 30.0%
ACATATAAGCATAACAAACC+AGG - chr2_4:55231588-55231607 None:intergenic 30.0%
! ATAGATTATCTCCTCCTTTT+TGG - chr2_4:55229367-55229386 None:intergenic 30.0%
! GTAATGGAAAAACTTACTGT+TGG - chr2_4:55229478-55229497 None:intergenic 30.0%
ACAACGATATGATATGGTAA+TGG - chr2_4:55229494-55229513 None:intergenic 30.0%
!! ACTATCTGCTCTAAGAATTA+AGG + chr2_4:55229568-55229587 Msa0335960:intron 30.0%
ATGTTTATGATGTCTTAGCT+CGG - chr2_4:55229799-55229818 None:intergenic 30.0%
! CAGATGCATTTTATTAGTGT+TGG - chr2_4:55229888-55229907 None:intergenic 30.0%
CTCACAAATATTTATGTCAC+TGG - chr2_4:55230138-55230157 None:intergenic 30.0%
AGACTACTTCGAAAAGTTTA+TGG + chr2_4:55230156-55230175 Msa0335960:intron 30.0%
TATAGTTCAGTTCTTTGGTT+GGG + chr2_4:55230444-55230463 Msa0335960:intron 30.0%
AATTCGATGTCTAACTAAGA+GGG - chr2_4:55230544-55230563 None:intergenic 30.0%
TCTTAGTTAGACATCGAATT+CGG + chr2_4:55230544-55230563 Msa0335960:intron 30.0%
! TCATTTATTCCTTTTGTGTG+TGG + chr2_4:55230659-55230678 Msa0335960:intron 30.0%
ATCTTATACAAAACACAGCA+GGG - chr2_4:55230993-55231012 None:intergenic 30.0%
! AAGAATGTTTCACGATCTTT+TGG + chr2_4:55231162-55231181 Msa0335960:CDS 30.0%
GCTTACAAAATTAAGCTGAT+TGG + chr2_4:55231225-55231244 Msa0335960:CDS 30.0%
TCTCGATGACAATCAATTAA+AGG + chr2_4:55231413-55231432 Msa0335960:CDS 30.0%
GATGACAATCAATTAAAGGA+AGG + chr2_4:55231417-55231436 Msa0335960:CDS 30.0%
! TTGGGAGAAATTTTCAATAG+AGG - chr2_4:55231509-55231528 None:intergenic 30.0%
ACATATAAGCATAACAAACC+AGG - chr2_4:55231588-55231607 None:intergenic 30.0%
!!! AAGAAGGCGTTTTGTATTGT+CGG - chr2_4:55229445-55229464 None:intergenic 35.0%
!!! CCTAACAATGTCGGTTTTAT+GGG - chr2_4:55229926-55229945 None:intergenic 35.0%
CGGTTATCTTGTAAAGCTTA+TGG - chr2_4:55230223-55230242 None:intergenic 35.0%
CATGCAAGTGCTTACTTATA+AGG - chr2_4:55230305-55230324 None:intergenic 35.0%
GTAGGTATAGTTCAGTTCTT+TGG + chr2_4:55230439-55230458 Msa0335960:intron 35.0%
GTATAGTTCAGTTCTTTGGT+TGG + chr2_4:55230443-55230462 Msa0335960:intron 35.0%
GAATTCGATGTCTAACTAAG+AGG - chr2_4:55230545-55230564 None:intergenic 35.0%
TCTTATACAAAACACAGCAG+GGG - chr2_4:55230992-55231011 None:intergenic 35.0%
CATCTTATACAAAACACAGC+AGG - chr2_4:55230994-55231013 None:intergenic 35.0%
! CAAAATTAAGCTGATTGGTC+CGG + chr2_4:55231230-55231249 Msa0335960:CDS 35.0%
! TGAATAACACAGTCTGAAGT+TGG - chr2_4:55231468-55231487 None:intergenic 35.0%
GTTATTCATACGCACAAAGA+AGG + chr2_4:55231480-55231499 Msa0335960:CDS 35.0%
!!! AAGAAGGCGTTTTGTATTGT+CGG - chr2_4:55229445-55229464 None:intergenic 35.0%
!!! CCTAACAATGTCGGTTTTAT+GGG - chr2_4:55229926-55229945 None:intergenic 35.0%
CGGTTATCTTGTAAAGCTTA+TGG - chr2_4:55230223-55230242 None:intergenic 35.0%
CATGCAAGTGCTTACTTATA+AGG - chr2_4:55230305-55230324 None:intergenic 35.0%
GTAGGTATAGTTCAGTTCTT+TGG + chr2_4:55230439-55230458 Msa0335960:intron 35.0%
GTATAGTTCAGTTCTTTGGT+TGG + chr2_4:55230443-55230462 Msa0335960:intron 35.0%
GAATTCGATGTCTAACTAAG+AGG - chr2_4:55230545-55230564 None:intergenic 35.0%
TCTTATACAAAACACAGCAG+GGG - chr2_4:55230992-55231011 None:intergenic 35.0%
CATCTTATACAAAACACAGC+AGG - chr2_4:55230994-55231013 None:intergenic 35.0%
! CAAAATTAAGCTGATTGGTC+CGG + chr2_4:55231230-55231249 Msa0335960:CDS 35.0%
! TGAATAACACAGTCTGAAGT+TGG - chr2_4:55231468-55231487 None:intergenic 35.0%
GTTATTCATACGCACAAAGA+AGG + chr2_4:55231480-55231499 Msa0335960:CDS 35.0%
!!! TATCTCCTCCTTTTTGGCTT+GGG - chr2_4:55229361-55229380 None:intergenic 40.0%
!!! TTATCTCCTCCTTTTTGGCT+TGG - chr2_4:55229362-55229381 None:intergenic 40.0%
CTTTCGTTCAATCGTTCGTT+TGG + chr2_4:55229389-55229408 Msa0335960:intron 40.0%
! TGTTGGTGAAACGATGAAGA+AGG - chr2_4:55229461-55229480 None:intergenic 40.0%
TGGGACGAACGTCAAATTAA+TGG - chr2_4:55229754-55229773 None:intergenic 40.0%
! TTAATTTGACGTTCGTCCCA+AGG + chr2_4:55229754-55229773 Msa0335960:intron 40.0%
!! GAGTTTGGTCTTGTAACCTT+GGG - chr2_4:55229773-55229792 None:intergenic 40.0%
!! AGAGTTTGGTCTTGTAACCT+TGG - chr2_4:55229774-55229793 None:intergenic 40.0%
!! TCGGTTTTATGGGTTGTGTT+AGG - chr2_4:55229916-55229935 None:intergenic 40.0%
CCCATAAAACCGACATTGTT+AGG + chr2_4:55229923-55229942 Msa0335960:intron 40.0%
!!! GCCTAACAATGTCGGTTTTA+TGG - chr2_4:55229927-55229946 None:intergenic 40.0%
TAAAACCGACATTGTTAGGC+TGG + chr2_4:55229927-55229946 Msa0335960:intron 40.0%
AAAACCGACATTGTTAGGCT+GGG + chr2_4:55229928-55229947 Msa0335960:intron 40.0%
! ACTCGACATTTTCCACTTTG+TGG + chr2_4:55229976-55229995 Msa0335960:intron 40.0%
! CTCGACATTTTCCACTTTGT+GGG + chr2_4:55229977-55229996 Msa0335960:intron 40.0%
TGCATCGAAAAGAACTTCAC+TGG - chr2_4:55230088-55230107 None:intergenic 40.0%
GTGTGTAGATTGTAGACAGT+AGG + chr2_4:55230421-55230440 Msa0335960:intron 40.0%
AACATGCTTGCACACAAGAA+GGG - chr2_4:55230494-55230513 None:intergenic 40.0%
AAACATGCTTGCACACAAGA+AGG - chr2_4:55230495-55230514 None:intergenic 40.0%
! AACTAAGAGGGATTCTGGAA+AGG - chr2_4:55230532-55230551 None:intergenic 40.0%
TGTCTAACTAAGAGGGATTC+TGG - chr2_4:55230537-55230556 None:intergenic 40.0%
TCGGTAGGATCAAAAGAACT+GGG + chr2_4:55230611-55230630 Msa0335960:intron 40.0%
TCATGCAATCCACACACAAA+AGG - chr2_4:55230671-55230690 None:intergenic 40.0%
AATAACTCGATTGCTACAGC+AGG - chr2_4:55230858-55230877 None:intergenic 40.0%
AACAACAGTAGAAAGCAAGC+TGG - chr2_4:55230934-55230953 None:intergenic 40.0%
GTAAACCTTCACAGTTGACA+AGG - chr2_4:55231058-55231077 None:intergenic 40.0%
!!! CAGTTTTAAGCGTGCAATAG+CGG - chr2_4:55231080-55231099 None:intergenic 40.0%
!! CGGCAGTAGTGAATTGATTT+TGG - chr2_4:55231107-55231126 None:intergenic 40.0%
TAGATGAAGCAGATGACTTC+AGG - chr2_4:55231188-55231207 None:intergenic 40.0%
TGAAGTCATCTGCTTCATCT+AGG + chr2_4:55231187-55231206 Msa0335960:CDS 40.0%
GAAGTCATCTGCTTCATCTA+GGG + chr2_4:55231188-55231207 Msa0335960:CDS 40.0%
TGAATACGGAAGTTCAACTC+CGG - chr2_4:55231319-55231338 None:intergenic 40.0%
TGAAGTTGGATAAGCTACAC+AGG - chr2_4:55231454-55231473 None:intergenic 40.0%
TAAGCATAACAAACCAGGTC+TGG - chr2_4:55231583-55231602 None:intergenic 40.0%
!!! TATCTCCTCCTTTTTGGCTT+GGG - chr2_4:55229361-55229380 None:intergenic 40.0%
!!! TTATCTCCTCCTTTTTGGCT+TGG - chr2_4:55229362-55229381 None:intergenic 40.0%
CTTTCGTTCAATCGTTCGTT+TGG + chr2_4:55229389-55229408 Msa0335960:intron 40.0%
! TGTTGGTGAAACGATGAAGA+AGG - chr2_4:55229461-55229480 None:intergenic 40.0%
TGGGACGAACGTCAAATTAA+TGG - chr2_4:55229754-55229773 None:intergenic 40.0%
! TTAATTTGACGTTCGTCCCA+AGG + chr2_4:55229754-55229773 Msa0335960:intron 40.0%
!! GAGTTTGGTCTTGTAACCTT+GGG - chr2_4:55229773-55229792 None:intergenic 40.0%
!! AGAGTTTGGTCTTGTAACCT+TGG - chr2_4:55229774-55229793 None:intergenic 40.0%
!! TCGGTTTTATGGGTTGTGTT+AGG - chr2_4:55229916-55229935 None:intergenic 40.0%
CCCATAAAACCGACATTGTT+AGG + chr2_4:55229923-55229942 Msa0335960:intron 40.0%
!!! GCCTAACAATGTCGGTTTTA+TGG - chr2_4:55229927-55229946 None:intergenic 40.0%
TAAAACCGACATTGTTAGGC+TGG + chr2_4:55229927-55229946 Msa0335960:intron 40.0%
AAAACCGACATTGTTAGGCT+GGG + chr2_4:55229928-55229947 Msa0335960:intron 40.0%
! ACTCGACATTTTCCACTTTG+TGG + chr2_4:55229976-55229995 Msa0335960:intron 40.0%
! CTCGACATTTTCCACTTTGT+GGG + chr2_4:55229977-55229996 Msa0335960:intron 40.0%
TGCATCGAAAAGAACTTCAC+TGG - chr2_4:55230088-55230107 None:intergenic 40.0%
GTGTGTAGATTGTAGACAGT+AGG + chr2_4:55230421-55230440 Msa0335960:intron 40.0%
AACATGCTTGCACACAAGAA+GGG - chr2_4:55230494-55230513 None:intergenic 40.0%
AAACATGCTTGCACACAAGA+AGG - chr2_4:55230495-55230514 None:intergenic 40.0%
! AACTAAGAGGGATTCTGGAA+AGG - chr2_4:55230532-55230551 None:intergenic 40.0%
TGTCTAACTAAGAGGGATTC+TGG - chr2_4:55230537-55230556 None:intergenic 40.0%
TCGGTAGGATCAAAAGAACT+GGG + chr2_4:55230611-55230630 Msa0335960:intron 40.0%
TCATGCAATCCACACACAAA+AGG - chr2_4:55230671-55230690 None:intergenic 40.0%
AATAACTCGATTGCTACAGC+AGG - chr2_4:55230858-55230877 None:intergenic 40.0%
AACAACAGTAGAAAGCAAGC+TGG - chr2_4:55230934-55230953 None:intergenic 40.0%
GTAAACCTTCACAGTTGACA+AGG - chr2_4:55231058-55231077 None:intergenic 40.0%
!!! CAGTTTTAAGCGTGCAATAG+CGG - chr2_4:55231080-55231099 None:intergenic 40.0%
!! CGGCAGTAGTGAATTGATTT+TGG - chr2_4:55231107-55231126 None:intergenic 40.0%
TAGATGAAGCAGATGACTTC+AGG - chr2_4:55231188-55231207 None:intergenic 40.0%
TGAAGTCATCTGCTTCATCT+AGG + chr2_4:55231187-55231206 Msa0335960:CDS 40.0%
GAAGTCATCTGCTTCATCTA+GGG + chr2_4:55231188-55231207 Msa0335960:CDS 40.0%
TGAATACGGAAGTTCAACTC+CGG - chr2_4:55231319-55231338 None:intergenic 40.0%
TGAAGTTGGATAAGCTACAC+AGG - chr2_4:55231454-55231473 None:intergenic 40.0%
TAAGCATAACAAACCAGGTC+TGG - chr2_4:55231583-55231602 None:intergenic 40.0%
CATCGTTGTACTGTGAAGAC+AGG + chr2_4:55229543-55229562 Msa0335960:intron 45.0%
GTCTTAGCTCGGTAAGAGTT+TGG - chr2_4:55229788-55229807 None:intergenic 45.0%
TGTGGGTGTGTGTGAGTTTA+TGG + chr2_4:55229994-55230013 Msa0335960:intron 45.0%
GACATCGAATTCGGACCTAA+TGG + chr2_4:55230553-55230572 Msa0335960:intron 45.0%
ATCGAATTCGGACCTAATGG+AGG + chr2_4:55230556-55230575 Msa0335960:intron 45.0%
AATTCGGACCTAATGGAGGT+TGG + chr2_4:55230560-55230579 Msa0335960:intron 45.0%
CTCACAATCCAACCTCCATT+AGG - chr2_4:55230571-55230590 None:intergenic 45.0%
TAGTCGACCCTGTTAAGACT+CGG + chr2_4:55230592-55230611 Msa0335960:intron 45.0%
GATCCTACCGAGTCTTAACA+GGG - chr2_4:55230602-55230621 None:intergenic 45.0%
TGATCCTACCGAGTCTTAAC+AGG - chr2_4:55230603-55230622 None:intergenic 45.0%
CTCGGTAGGATCAAAAGAAC+TGG + chr2_4:55230610-55230629 Msa0335960:intron 45.0%
ATTCTTCGCAGATCCTAGAG+TGG - chr2_4:55231148-55231167 None:intergenic 45.0%
! TTCATCTAGGGTTACTGCAG+TGG + chr2_4:55231200-55231219 Msa0335960:CDS 45.0%
! ATTAAGCTGATTGGTCCGGA+TGG + chr2_4:55231234-55231253 Msa0335960:CDS 45.0%
TTAGATGCAGCAGAGAATGC+CGG + chr2_4:55231297-55231316 Msa0335960:CDS 45.0%
!! CAACAAGGGCACAGGTTTTT+GGG - chr2_4:55231527-55231546 None:intergenic 45.0%
CAAAAACCTGTGCCCTTGTT+GGG + chr2_4:55231526-55231545 Msa0335960:three_prime_UTR 45.0%
AAAAACCTGTGCCCTTGTTG+GGG + chr2_4:55231527-55231546 Msa0335960:three_prime_UTR 45.0%
TTAGACATTACAGCCAGACC+TGG + chr2_4:55231567-55231586 Msa0335960:three_prime_UTR 45.0%
AGATAATGTGCGCTGTTGCT+TGG + chr2_4:55231681-55231700 Msa0335960:three_prime_UTR 45.0%
CATCGTTGTACTGTGAAGAC+AGG + chr2_4:55229543-55229562 Msa0335960:intron 45.0%
GTCTTAGCTCGGTAAGAGTT+TGG - chr2_4:55229788-55229807 None:intergenic 45.0%
TGTGGGTGTGTGTGAGTTTA+TGG + chr2_4:55229994-55230013 Msa0335960:intron 45.0%
GACATCGAATTCGGACCTAA+TGG + chr2_4:55230553-55230572 Msa0335960:intron 45.0%
ATCGAATTCGGACCTAATGG+AGG + chr2_4:55230556-55230575 Msa0335960:intron 45.0%
AATTCGGACCTAATGGAGGT+TGG + chr2_4:55230560-55230579 Msa0335960:intron 45.0%
CTCACAATCCAACCTCCATT+AGG - chr2_4:55230571-55230590 None:intergenic 45.0%
TAGTCGACCCTGTTAAGACT+CGG + chr2_4:55230592-55230611 Msa0335960:intron 45.0%
GATCCTACCGAGTCTTAACA+GGG - chr2_4:55230602-55230621 None:intergenic 45.0%
TGATCCTACCGAGTCTTAAC+AGG - chr2_4:55230603-55230622 None:intergenic 45.0%
CTCGGTAGGATCAAAAGAAC+TGG + chr2_4:55230610-55230629 Msa0335960:intron 45.0%
ATTCTTCGCAGATCCTAGAG+TGG - chr2_4:55231148-55231167 None:intergenic 45.0%
! TTCATCTAGGGTTACTGCAG+TGG + chr2_4:55231200-55231219 Msa0335960:CDS 45.0%
! ATTAAGCTGATTGGTCCGGA+TGG + chr2_4:55231234-55231253 Msa0335960:CDS 45.0%
TTAGATGCAGCAGAGAATGC+CGG + chr2_4:55231297-55231316 Msa0335960:CDS 45.0%
!! CAACAAGGGCACAGGTTTTT+GGG - chr2_4:55231527-55231546 None:intergenic 45.0%
CAAAAACCTGTGCCCTTGTT+GGG + chr2_4:55231526-55231545 Msa0335960:three_prime_UTR 45.0%
AAAAACCTGTGCCCTTGTTG+GGG + chr2_4:55231527-55231546 Msa0335960:three_prime_UTR 45.0%
TTAGACATTACAGCCAGACC+TGG + chr2_4:55231567-55231586 Msa0335960:three_prime_UTR 45.0%
AGATAATGTGCGCTGTTGCT+TGG + chr2_4:55231681-55231700 Msa0335960:three_prime_UTR 45.0%
!!! CTCCTTTTTGGCTTGGGCTT+GGG - chr2_4:55229355-55229374 None:intergenic 50.0%
!! TTGTATTGTCGGAGAGCGCT+GGG - chr2_4:55229434-55229453 None:intergenic 50.0%
! TTTGTATTGTCGGAGAGCGC+TGG - chr2_4:55229435-55229454 None:intergenic 50.0%
GATGCCCAGCCTAACAATGT+CGG - chr2_4:55229935-55229954 None:intergenic 50.0%
ACTCACACACACCCACAAAG+TGG - chr2_4:55229991-55230010 None:intergenic 50.0%
GGGTGTGTGTGAGTTTATGG+TGG + chr2_4:55229997-55230016 Msa0335960:intron 50.0%
ACTCGATTGCTACAGCAGGT+AGG - chr2_4:55230854-55230873 None:intergenic 50.0%
! GTCAGCCTTGTCAACTGTGA+AGG + chr2_4:55231050-55231069 Msa0335960:CDS 50.0%
TCGCAGATCCTAGAGTGGAA+GGG - chr2_4:55231143-55231162 None:intergenic 50.0%
TTCGCAGATCCTAGAGTGGA+AGG - chr2_4:55231144-55231163 None:intergenic 50.0%
AGAAACAACCTTCCCAGCAC+AGG - chr2_4:55231364-55231383 None:intergenic 50.0%
! TGTGCTGGGAAGGTTGTTTC+TGG + chr2_4:55231363-55231382 Msa0335960:CDS 50.0%
GGTTCTGTCGATCAGTCAGA+TGG + chr2_4:55231384-55231403 Msa0335960:CDS 50.0%
!! CCAACAAGGGCACAGGTTTT+TGG - chr2_4:55231528-55231547 None:intergenic 50.0%
CCAAAAACCTGTGCCCTTGT+TGG + chr2_4:55231525-55231544 Msa0335960:three_prime_UTR 50.0%
AAAACCTGTGCCCTTGTTGG+GGG + chr2_4:55231528-55231547 Msa0335960:three_prime_UTR 50.0%
CTCGAAATCTCCCCCAACAA+GGG - chr2_4:55231541-55231560 None:intergenic 50.0%
ACTCGAAATCTCCCCCAACA+AGG - chr2_4:55231542-55231561 None:intergenic 50.0%
!!! CTCCTTTTTGGCTTGGGCTT+GGG - chr2_4:55229355-55229374 None:intergenic 50.0%
!! TTGTATTGTCGGAGAGCGCT+GGG - chr2_4:55229434-55229453 None:intergenic 50.0%
! TTTGTATTGTCGGAGAGCGC+TGG - chr2_4:55229435-55229454 None:intergenic 50.0%
GATGCCCAGCCTAACAATGT+CGG - chr2_4:55229935-55229954 None:intergenic 50.0%
ACTCACACACACCCACAAAG+TGG - chr2_4:55229991-55230010 None:intergenic 50.0%
GGGTGTGTGTGAGTTTATGG+TGG + chr2_4:55229997-55230016 Msa0335960:intron 50.0%
ACTCGATTGCTACAGCAGGT+AGG - chr2_4:55230854-55230873 None:intergenic 50.0%
! GTCAGCCTTGTCAACTGTGA+AGG + chr2_4:55231050-55231069 Msa0335960:CDS 50.0%
TCGCAGATCCTAGAGTGGAA+GGG - chr2_4:55231143-55231162 None:intergenic 50.0%
TTCGCAGATCCTAGAGTGGA+AGG - chr2_4:55231144-55231163 None:intergenic 50.0%
AGAAACAACCTTCCCAGCAC+AGG - chr2_4:55231364-55231383 None:intergenic 50.0%
! TGTGCTGGGAAGGTTGTTTC+TGG + chr2_4:55231363-55231382 Msa0335960:CDS 50.0%
GGTTCTGTCGATCAGTCAGA+TGG + chr2_4:55231384-55231403 Msa0335960:CDS 50.0%
!! CCAACAAGGGCACAGGTTTT+TGG - chr2_4:55231528-55231547 None:intergenic 50.0%
CCAAAAACCTGTGCCCTTGT+TGG + chr2_4:55231525-55231544 Msa0335960:three_prime_UTR 50.0%
AAAACCTGTGCCCTTGTTGG+GGG + chr2_4:55231528-55231547 Msa0335960:three_prime_UTR 50.0%
CTCGAAATCTCCCCCAACAA+GGG - chr2_4:55231541-55231560 None:intergenic 50.0%
ACTCGAAATCTCCCCCAACA+AGG - chr2_4:55231542-55231561 None:intergenic 50.0%
!! TTTGGCTTGGGCTTGGGCTT+GGG - chr2_4:55229349-55229368 None:intergenic 55.0%
!!! TTTTGGCTTGGGCTTGGGCT+TGG - chr2_4:55229350-55229369 None:intergenic 55.0%
AGCCCAAGCCCAAGCCAAAA+AGG + chr2_4:55229350-55229369 Msa0335960:intron 55.0%
!!! CCTCCTTTTTGGCTTGGGCT+TGG - chr2_4:55229356-55229375 None:intergenic 55.0%
CCAAGCCCAAGCCAAAAAGG+AGG + chr2_4:55229353-55229372 Msa0335960:intron 55.0%
GTTAGGCTGGGCATCTTTCG+TGG + chr2_4:55229940-55229959 Msa0335960:intron 55.0%
TGGGCATCTTTCGTGGTGTC+AGG + chr2_4:55229947-55229966 Msa0335960:intron 55.0%
! GGGCATCTTTCGTGGTGTCA+GGG + chr2_4:55229948-55229967 Msa0335960:intron 55.0%
! CGACCCTGTTAAGACTCGGT+AGG + chr2_4:55230596-55230615 Msa0335960:intron 55.0%
!! GGAAGGGCACTTGACTGTTG+CGG - chr2_4:55231127-55231146 None:intergenic 55.0%
GTCAAGTGCCCTTCCACTCT+AGG + chr2_4:55231132-55231151 Msa0335960:CDS 55.0%
CTTCCGTATTCATGCCGTGC+TGG + chr2_4:55231327-55231346 Msa0335960:CDS 55.0%
GCTCCAGCACGGCATGAATA+CGG - chr2_4:55231333-55231352 None:intergenic 55.0%
AGGTAGAGCATGCTCCAGCA+CGG - chr2_4:55231344-55231363 None:intergenic 55.0%
GAGCATGCTCTACCTGTGCT+GGG + chr2_4:55231349-55231368 Msa0335960:CDS 55.0%
ATGCTCTACCTGTGCTGGGA+AGG + chr2_4:55231353-55231372 Msa0335960:CDS 55.0%
!! TTTGGCTTGGGCTTGGGCTT+GGG - chr2_4:55229349-55229368 None:intergenic 55.0%
!!! TTTTGGCTTGGGCTTGGGCT+TGG - chr2_4:55229350-55229369 None:intergenic 55.0%
AGCCCAAGCCCAAGCCAAAA+AGG + chr2_4:55229350-55229369 Msa0335960:intron 55.0%
!!! CCTCCTTTTTGGCTTGGGCT+TGG - chr2_4:55229356-55229375 None:intergenic 55.0%
CCAAGCCCAAGCCAAAAAGG+AGG + chr2_4:55229353-55229372 Msa0335960:intron 55.0%
GTTAGGCTGGGCATCTTTCG+TGG + chr2_4:55229940-55229959 Msa0335960:intron 55.0%
TGGGCATCTTTCGTGGTGTC+AGG + chr2_4:55229947-55229966 Msa0335960:intron 55.0%
! GGGCATCTTTCGTGGTGTCA+GGG + chr2_4:55229948-55229967 Msa0335960:intron 55.0%
! CGACCCTGTTAAGACTCGGT+AGG + chr2_4:55230596-55230615 Msa0335960:intron 55.0%
!! GGAAGGGCACTTGACTGTTG+CGG - chr2_4:55231127-55231146 None:intergenic 55.0%
GTCAAGTGCCCTTCCACTCT+AGG + chr2_4:55231132-55231151 Msa0335960:CDS 55.0%
CTTCCGTATTCATGCCGTGC+TGG + chr2_4:55231327-55231346 Msa0335960:CDS 55.0%
GCTCCAGCACGGCATGAATA+CGG - chr2_4:55231333-55231352 None:intergenic 55.0%
AGGTAGAGCATGCTCCAGCA+CGG - chr2_4:55231344-55231363 None:intergenic 55.0%
GAGCATGCTCTACCTGTGCT+GGG + chr2_4:55231349-55231368 Msa0335960:CDS 55.0%
ATGCTCTACCTGTGCTGGGA+AGG + chr2_4:55231353-55231372 Msa0335960:CDS 55.0%
GGAGCATGCTCTACCTGTGC+TGG + chr2_4:55231348-55231367 Msa0335960:CDS 60.0%
! ATCTCCCCCAACAAGGGCAC+AGG - chr2_4:55231535-55231554 None:intergenic 60.0%
GGAGCATGCTCTACCTGTGC+TGG + chr2_4:55231348-55231367 Msa0335960:CDS 60.0%
! ATCTCCCCCAACAAGGGCAC+AGG - chr2_4:55231535-55231554 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 55229301 55231743 55229301 ID=Msa0335960;Name=Msa0335960
chr2_4 mRNA 55229301 55231743 55229301 ID=Msa0335960-mRNA-1;Parent=Msa0335960;Name=Msa0335960-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0.5|0.5|0|0.5|2|228|160
chr2_4 exon 55229301 55229308 55229301 ID=Msa0335960-mRNA-1:exon:16723;Parent=Msa0335960-mRNA-1
chr2_4 exon 55231041 55231743 55231041 ID=Msa0335960-mRNA-1:exon:16724;Parent=Msa0335960-mRNA-1
chr2_4 CDS 55229301 55229308 55229301 ID=Msa0335960-mRNA-1:cds;Parent=Msa0335960-mRNA-1
chr2_4 CDS 55231041 55231515 55231041 ID=Msa0335960-mRNA-1:cds;Parent=Msa0335960-mRNA-1
chr2_4 three_prime_UTR 55231516 55231743 55231516 ID=Msa0335960-mRNA-1:three_prime_utr;Parent=Msa0335960-mRNA-1
Gene Sequence

>Msa0335960

ATGGGGGTGGTAATCATGTCAGCCTTGTCAACTGTGAAGGTTTACCGCTATTGCACGCTTAAAACTGCTAACCAAAATCAATTCACTACTGCCGCAACAGTCAAGTGCCCTTCCACTCTAGGATCTGCGAAGAATGTTTCACGATCTTTTGGCCTGAAGTCATCTGCTTCATCTAGGGTTACTGCAGTGGCTGCTTACAAAATTAAGCTGATTGGTCCGGATGGAAAAGAAAATGAATTTGAAGCAACTGATGACACTTACATCTTAGATGCAGCAGAGAATGCCGGAGTTGAACTTCCGTATTCATGCCGTGCTGGAGCATGCTCTACCTGTGCTGGGAAGGTTGTTTCTGGTTCTGTCGATCAGTCAGATGGCTCATTTCTCGATGACAATCAATTAAAGGAAGGTTATGTTCTAACCTGTGTAGCTTATCCAACTTCAGACTGTGTTATTCATACGCACAAAGAAGGTGACCTCTATTGA
Protein sequence

>Msa0335960

MGVVIMSALSTVKVYRYCTLKTANQNQFTTAATVKCPSTLGSAKNVSRSFGLKSSASSRVTAVAAYKIKLIGPDGKENEFEATDDTYILDAAENAGVELPYSCRAGACSTCAGKVVSGSVDQSDGSFLDDNQLKEGYVLTCVAYPTSDCVIHTHKEGDLY*