AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0339350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0339350 MtrunA17_Chr2g0327781 100.000 154 0 0 1 154 1 154 3.29e-107 302
Msa0339350 MtrunA17_Chr4g0030821 95.364 151 6 1 4 154 3 152 4.20e-81 236
Msa0339350 MtrunA17_Chr7g0222741 93.506 154 10 0 1 154 1 154 3.56e-71 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0339350 AT3G55280.1 87.662 154 19 0 1 154 1 154 1.89e-71 212
Msa0339350 AT3G55280.2 87.662 154 19 0 1 154 1 154 1.89e-71 212
Msa0339350 AT2G39460.1 87.662 154 19 0 1 154 1 154 3.05e-68 204
Msa0339350 AT2G39460.2 87.662 154 19 0 1 154 1 154 3.05e-68 204
Msa0339350 AT3G55280.3 93.220 118 8 0 37 154 31 148 4.61e-67 201

Find 93 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAGATTGGGATTATTTA+AGG 0.136899 2_4:+60489002 Msa0339350:CDS
AGTTCGATTGCAATTAAATT+TGG 0.183176 2_4:+60489148 Msa0339350:three_prime_UTR
CAGCCAAGTGTTCCATTTAT+AGG 0.202696 2_4:-60489600 None:intergenic
CTTCTTTGCCTTCTTCTTGA+TGG 0.213233 2_4:-60488357 None:intergenic
GCACTAATCCGAGGGTATTT+TGG 0.229197 2_4:-60488434 None:intergenic
GATCAAGGTGTTAACTTTCT+TGG 0.259114 2_4:-60488624 None:intergenic
CAACCTTACATAGGCCTTCT+TGG 0.259824 2_4:-60488949 None:intergenic
CATATGGTTTAGCCTATAAA+TGG 0.287603 2_4:+60489588 Msa0339350:three_prime_UTR
TCACGCAGGTTTCACATCTA+AGG 0.288210 2_4:+60486847 Msa0339350:five_prime_UTR
AGATGGTAATCTTGAGAATT+AGG 0.291366 2_4:-60489343 None:intergenic
AAGATCTGTGCTGTCACATT+TGG 0.315998 2_4:+60489399 Msa0339350:three_prime_UTR
CTACGTATCATGCTGAATTC+TGG 0.316796 2_4:+60489746 Msa0339350:three_prime_UTR
GCTTTCAAGGCCTGTGCCTT+TGG 0.317092 2_4:-60488308 None:intergenic
ATTAATTTCAGTAGTTCTAT+AGG 0.318193 2_4:+60489706 Msa0339350:three_prime_UTR
CTATCCCTAGATAAAGTCTT+TGG 0.341250 2_4:-60488407 None:intergenic
CACTGCTTTCGCAGCTTTCA+AGG 0.342192 2_4:-60488321 None:intergenic
TTACGATGTCCTTGCACATA+TGG 0.359904 2_4:+60489572 Msa0339350:three_prime_UTR
CTTCATCTTCTCCGATTCAA+TGG 0.360156 2_4:+60486874 Msa0339350:exon
CCTACTTGCTTAGCATTATG+AGG 0.361512 2_4:-60489450 None:intergenic
CAAGATTACCATCTCTTAAG+AGG 0.384533 2_4:+60489352 Msa0339350:three_prime_UTR
ACAGGCCAAAGACTTTATCT+AGG 0.384705 2_4:+60488402 Msa0339350:CDS
ATTGGGATTATTTAAGGTAA+TGG 0.385537 2_4:+60489008 Msa0339350:exon
AACAAGTAGGCAAATGGTCT+GGG 0.408451 2_4:+60489520 Msa0339350:three_prime_UTR
GAACAAGTAGGCAAATGGTC+TGG 0.411419 2_4:+60489519 Msa0339350:three_prime_UTR
GACACCTGATTATGATGCTT+TGG 0.412386 2_4:+60488971 Msa0339350:CDS
GAGAATTAGGTAGATGGCTT+AGG 0.412782 2_4:-60489330 None:intergenic
TTGGATGTTGCAAACAAGAT+TGG 0.413392 2_4:+60488990 Msa0339350:CDS
CCTACTTGTTCAGCATTATG+AGG 0.414136 2_4:-60489507 None:intergenic
AATCTTGAGAATTAGGTAGA+TGG 0.416006 2_4:-60489336 None:intergenic
CACAATAACAAAACACAATC+CGG 0.420029 2_4:-60489119 None:intergenic
AGATCTGTGCTGTCACATTT+GGG 0.421441 2_4:+60489400 Msa0339350:three_prime_UTR
TAGGTAGATGGCTTAGGCTT+AGG 0.421556 2_4:-60489324 None:intergenic
GCTGCGAAAGCAGTGAAATC+AGG 0.425217 2_4:+60488329 Msa0339350:CDS
TGGATGTTGCAAACAAGATT+GGG 0.427718 2_4:+60488991 Msa0339350:CDS
ATAGGCCTTCTTGGTGCCAT+CGG 0.428001 2_4:-60488940 None:intergenic
AGAAGAAGGCAAAGAAGATC+AGG 0.439428 2_4:+60488363 Msa0339350:CDS
TTTGACGCAAAGTTAAATTC+CGG 0.442084 2_4:+60489100 Msa0339350:three_prime_UTR
TTAGGCTTAGGCTGAGTTGA+TGG 0.447685 2_4:-60489306 None:intergenic
CAAGGCCTGTGCCTTTGGAT+CGG 0.452797 2_4:-60488303 None:intergenic
AGGTGTTAACTTTCTTGGCC+TGG 0.453520 2_4:-60488619 None:intergenic
GGATTATTTAAGGTAATGGC+TGG 0.458048 2_4:+60489012 Msa0339350:exon
ACATCCAAAGCATCATAATC+AGG 0.461568 2_4:-60488975 None:intergenic
CACGCAGGTTTCACATCTAA+GGG 0.470668 2_4:+60486848 Msa0339350:five_prime_UTR
GATGGCTTAGGCTTAGGCTT+AGG 0.477823 2_4:-60489318 None:intergenic
GATTCAATGGCTCCACCAAA+AGG 0.493121 2_4:+60486887 Msa0339350:exon
GTAACTGAGGTTGGTGGTCA+AGG 0.493313 2_4:+60489543 Msa0339350:three_prime_UTR
ATGCTGAACAAGTAGGCAAA+TGG 0.493728 2_4:+60489514 Msa0339350:three_prime_UTR
TAGGCCTTCTTGGTGCCATC+GGG 0.494781 2_4:-60488939 None:intergenic
ATGCTAAGCAAGTAGGCAAA+AGG 0.497527 2_4:+60489457 Msa0339350:three_prime_UTR
TAATGATCAAGTTTGTTCCT+TGG 0.498760 2_4:-60488461 None:intergenic
AGAAAGTTAACACCTTGATC+AGG 0.514403 2_4:+60488627 Msa0339350:CDS
GATTGAAGACAACAACACTT+TGG 0.518542 2_4:+60488529 Msa0339350:CDS
CAAAGACTTTATCTAGGGAT+AGG 0.519733 2_4:+60488408 Msa0339350:CDS
GAAGAAAATGTACGACATCC+AGG 0.525002 2_4:+60488601 Msa0339350:CDS
CCTAAAGGCTTACCTGATCA+AGG 0.532987 2_4:-60488639 None:intergenic
TCTGGGGTAACTGAGGTTGG+TGG 0.536382 2_4:+60489537 Msa0339350:three_prime_UTR
AGGCACTGCCATCAAGAAGA+AGG 0.536388 2_4:+60488349 Msa0339350:CDS
GCTGAGCTAGTTTGAAAATG+TGG 0.543049 2_4:+60489619 Msa0339350:three_prime_UTR
CAGGCCCGATGGCACCAAGA+AGG 0.551305 2_4:+60488935 Msa0339350:intron
TAGCCTATAAATGGAACACT+TGG 0.552070 2_4:+60489597 Msa0339350:three_prime_UTR
GCACCAAGAAGGCCTATGTA+AGG 0.556146 2_4:+60488946 Msa0339350:CDS
TGGTCTGGGGTAACTGAGGT+TGG 0.557367 2_4:+60489534 Msa0339350:three_prime_UTR
GTATGATCAGTAATTTATGT+TGG 0.560241 2_4:+60489675 Msa0339350:three_prime_UTR
CAGGCCAAAGACTTTATCTA+GGG 0.565675 2_4:+60488403 Msa0339350:CDS
GAAGACCGATCCAAAGGCAC+AGG 0.567558 2_4:+60488298 Msa0339350:CDS
ACTGTCTGCTTCCACCACAC+AGG 0.577415 2_4:+60486822 Msa0339350:five_prime_UTR
AACAAAGTCCTCTTAAGAGA+TGG 0.577741 2_4:-60489360 None:intergenic
CCTCATAATGCTAAGCAAGT+AGG 0.580047 2_4:+60489450 Msa0339350:three_prime_UTR
TAGATAAAGTCTTTGGCCTG+TGG 0.590574 2_4:-60488400 None:intergenic
GGACAACTGTTACATTCCAC+AGG 0.605601 2_4:+60488384 Msa0339350:CDS
TGCAAAGAAGACCGATCCAA+AGG 0.612710 2_4:+60488292 Msa0339350:CDS
CCTCATAATGCTGAACAAGT+AGG 0.614601 2_4:+60489507 Msa0339350:three_prime_UTR
CAAAAGGTCTGCGGTAACCA+AGG 0.622635 2_4:+60489473 Msa0339350:three_prime_UTR
TACGTAGTATGACAACATCC+AGG 0.622710 2_4:-60489655 None:intergenic
ATAGGAATCCAAAATACCCT+CGG 0.629797 2_4:+60488426 Msa0339350:CDS
ATCAGGTGTCAACCTTACAT+AGG 0.633043 2_4:-60488958 None:intergenic
TAGGCTAAACCATATGTGCA+AGG 0.641503 2_4:-60489581 None:intergenic
CAAATGGTCTGGGGTAACTG+AGG 0.649166 2_4:+60489530 Msa0339350:three_prime_UTR
ATTGCTGACTCAGTTGTGAG+CGG 0.651573 2_4:-60488500 None:intergenic
ATGTGCTGTGAACATAATTG+AGG 0.658732 2_4:+60489257 Msa0339350:three_prime_UTR
TTGGTGTAGCACTAATCCGA+GGG 0.658734 2_4:-60488442 None:intergenic
CTTGGTGTAGCACTAATCCG+AGG 0.662347 2_4:-60488443 None:intergenic
ACGCAGGTTTCACATCTAAG+GGG 0.669567 2_4:+60486849 Msa0339350:five_prime_UTR
CAGAATTCAGCATGATACGT+AGG 0.673653 2_4:-60489745 None:intergenic
GCAAGTAGGCAAAAGGTCTG+CGG 0.678922 2_4:+60489464 Msa0339350:three_prime_UTR
ACAAGTAGGCAAATGGTCTG+GGG 0.680323 2_4:+60489521 Msa0339350:three_prime_UTR
GCGTCAAAATGTCTCCACAA+TGG 0.680459 2_4:-60489085 None:intergenic
CCTGCGTGAAACCTGTGTGG+TGG 0.684452 2_4:-60486833 None:intergenic
CCACCACACAGGTTTCACGC+AGG 0.685417 2_4:+60486833 Msa0339350:five_prime_UTR
ATTTGGGAGATATATACCAA+AGG 0.695220 2_4:+60489416 Msa0339350:three_prime_UTR
CTCGGATTAGTGCTACACCA+AGG 0.713270 2_4:+60488444 Msa0339350:CDS
AAACCTGCGTGAAACCTGTG+TGG 0.748070 2_4:-60486836 None:intergenic
TGTGTGGTGGAAGCAGACAG+TGG 0.760336 2_4:-60486820 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATCTCATTTTTATGTTA+TGG + chr2_4:60487160-60487179 Msa0339350:intron 15.0%
!!! AGTAGAACTTAAAATTTTAT+AGG + chr2_4:60487369-60487388 Msa0339350:intron 15.0%
!!! TTATTTTGGTTTTGTTAAAA+TGG - chr2_4:60487794-60487813 None:intergenic 15.0%
!!! TTATTTTGGTTTTGTTAAAA+TGG + chr2_4:60487794-60487813 Msa0339350:intron 15.0%
!!! TTCTTTTCTTTTTCAATAGT+CGG + chr2_4:60486924-60486943 Msa0339350:intron 20.0%
!!! TAAATATTGGTTTATCTACA+CGG + chr2_4:60487045-60487064 Msa0339350:intron 20.0%
!! ATATAGCAAAAGATCTAAAT+TGG - chr2_4:60487339-60487358 None:intergenic 20.0%
!! ATAATAAAGAAATGAGAATG+CGG - chr2_4:60487438-60487457 None:intergenic 20.0%
!!! GTTTTGATTAAAAAAATTGC+TGG - chr2_4:60487593-60487612 None:intergenic 20.0%
!!! TTTTGTTGAATAGAAGATTT+AGG + chr2_4:60487921-60487940 Msa0339350:intron 20.0%
!!! AGCTATTTTGTATACATAAA+AGG - chr2_4:60488207-60488226 None:intergenic 20.0%
!! CTCATGTTATTATTTACAAT+GGG - chr2_4:60488864-60488883 None:intergenic 20.0%
!! TCTCATGTTATTATTTACAA+TGG - chr2_4:60488865-60488884 None:intergenic 20.0%
!!! TTTAGATGTTAGTCTTTTTA+TGG + chr2_4:60489056-60489075 Msa0339350:three_prime_UTR 20.0%
!!! ATTAATTTCAGTAGTTCTAT+AGG + chr2_4:60489706-60489725 Msa0339350:three_prime_UTR 20.0%
! GACTATTGAAAAAGAAAAGA+AGG - chr2_4:60486925-60486944 None:intergenic 25.0%
!!! GATTATATAGCAATTTTAGC+TGG - chr2_4:60487146-60487165 None:intergenic 25.0%
! TAATAAAGAAATGAGAATGC+GGG - chr2_4:60487437-60487456 None:intergenic 25.0%
!!! GTTTTGATTAAAAAAGTTGC+TGG + chr2_4:60487753-60487772 Msa0339350:intron 25.0%
! AATAAGTTGTTTATCCAAAC+AGG + chr2_4:60488180-60488199 Msa0339350:intron 25.0%
!! CAAATTTGTTCAGTAATTCA+TGG + chr2_4:60488677-60488696 Msa0339350:intron 25.0%
! AAACTGCTTTACAATAATGT+GGG - chr2_4:60488894-60488913 None:intergenic 25.0%
! TAAACTGCTTTACAATAATG+TGG - chr2_4:60488895-60488914 None:intergenic 25.0%
! AACAAGATTGGGATTATTTA+AGG + chr2_4:60489002-60489021 Msa0339350:CDS 25.0%
!! ATTGGGATTATTTAAGGTAA+TGG + chr2_4:60489008-60489027 Msa0339350:exon 25.0%
! ATCTAAACAAAAACTGTCTT+TGG - chr2_4:60489043-60489062 None:intergenic 25.0%
! AGTTCGATTGCAATTAAATT+TGG + chr2_4:60489148-60489167 Msa0339350:three_prime_UTR 25.0%
! AAATCTAAAAACAAACTTCG+AGG - chr2_4:60489213-60489232 None:intergenic 25.0%
!!! TCGAAGTTTGTTTTTAGATT+TGG + chr2_4:60489212-60489231 Msa0339350:three_prime_UTR 25.0%
!!! AAGTTTGTTTTTAGATTTGG+TGG + chr2_4:60489215-60489234 Msa0339350:three_prime_UTR 25.0%
! GTATGATCAGTAATTTATGT+TGG + chr2_4:60489675-60489694 Msa0339350:three_prime_UTR 25.0%
! ATAGATGAAATACCTTTTGG+TGG - chr2_4:60486902-60486921 None:intergenic 30.0%
! AGGATAGATGAAATACCTTT+TGG - chr2_4:60486905-60486924 None:intergenic 30.0%
ATGAAAACAGATGCAGAAAT+TGG - chr2_4:60486976-60486995 None:intergenic 30.0%
! CGAGTTTCTGGATTAAATAT+TGG + chr2_4:60487032-60487051 Msa0339350:intron 30.0%
!! AATAATCTGTGTTTTTGTGC+CGG + chr2_4:60487076-60487095 Msa0339350:intron 30.0%
!! GTTGTTTTCAGAAGCAATTT+TGG - chr2_4:60487299-60487318 None:intergenic 30.0%
!!! TGATTTGCTTGGTCTTATTT+TGG - chr2_4:60487566-60487585 None:intergenic 30.0%
!!! TGATTTGCTTGGACTTATTT+TGG + chr2_4:60487780-60487799 Msa0339350:intron 30.0%
! ATCTCCATGATTATTGCTTT+TGG + chr2_4:60487832-60487851 Msa0339350:intron 30.0%
AACACCAAAAGCAATAATCA+TGG - chr2_4:60487839-60487858 None:intergenic 30.0%
!!! TCTGGATGCTTCATTTTTTT+GGG + chr2_4:60487876-60487895 Msa0339350:intron 30.0%
!! TAGAAGATTTAGGACCTTTT+TGG + chr2_4:60487931-60487950 Msa0339350:intron 30.0%
!!! ATTTGTTTTGTGAGACTGTT+TGG + chr2_4:60487982-60488001 Msa0339350:intron 30.0%
!!! TTTGTTTTGTGAGACTGTTT+GGG + chr2_4:60487983-60488002 Msa0339350:intron 30.0%
TAAGCTATTCTTGTGCAAAT+GGG - chr2_4:60488118-60488137 None:intergenic 30.0%
ATAAGCTATTCTTGTGCAAA+TGG - chr2_4:60488119-60488138 None:intergenic 30.0%
TATACATAAAAGGTCCTGTT+TGG - chr2_4:60488197-60488216 None:intergenic 30.0%
! GGTCATCTTTTTCTTTTTGT+AGG + chr2_4:60488263-60488282 Msa0339350:intron 30.0%
! CTTTTTCTTTTTGTAGGTGA+TGG + chr2_4:60488269-60488288 Msa0339350:intron 30.0%
! TAATGATCAAGTTTGTTCCT+TGG - chr2_4:60488464-60488483 None:intergenic 30.0%
AGCTGACAAAAAGAAAATCA+AGG + chr2_4:60488571-60488590 Msa0339350:CDS 30.0%
CAAAAAAGATCACAACCTAA+AGG - chr2_4:60488657-60488676 None:intergenic 30.0%
GTAATAACATGTACATACAC+TGG + chr2_4:60488775-60488794 Msa0339350:intron 30.0%
!!! TGTACATACACTGGTTTTTA+TGG + chr2_4:60488784-60488803 Msa0339350:intron 30.0%
TATCATATCCCTTCACAATA+GGG - chr2_4:60488830-60488849 None:intergenic 30.0%
TTTGACGCAAAGTTAAATTC+CGG + chr2_4:60489100-60489119 Msa0339350:three_prime_UTR 30.0%
CACAATAACAAAACACAATC+CGG - chr2_4:60489122-60489141 None:intergenic 30.0%
AATCTTGAGAATTAGGTAGA+TGG - chr2_4:60489339-60489358 None:intergenic 30.0%
AGATGGTAATCTTGAGAATT+AGG - chr2_4:60489346-60489365 None:intergenic 30.0%
ATTTGGGAGATATATACCAA+AGG + chr2_4:60489416-60489435 Msa0339350:three_prime_UTR 30.0%
CATATGGTTTAGCCTATAAA+TGG + chr2_4:60489588-60489607 Msa0339350:three_prime_UTR 30.0%
TCTACTCGAATCAAATCTAC+CGG - chr2_4:60487098-60487117 None:intergenic 35.0%
!!! AGCAATTTTAGCTGGCATTA+AGG - chr2_4:60487138-60487157 None:intergenic 35.0%
GACCTCAAAAGCAATAATCT+TGG + chr2_4:60487507-60487526 Msa0339350:intron 35.0%
!! CTCCAAGATTATTGCTTTTG+AGG - chr2_4:60487512-60487531 None:intergenic 35.0%
! AATGAAGCATCCAGAGAAAT+GGG - chr2_4:60487871-60487890 None:intergenic 35.0%
! AAATGAAGCATCCAGAGAAA+TGG - chr2_4:60487872-60487891 None:intergenic 35.0%
!!! CTCTGGATGCTTCATTTTTT+TGG + chr2_4:60487875-60487894 Msa0339350:intron 35.0%
!! GATTTAGGACCTTTTTGGAT+TGG + chr2_4:60487936-60487955 Msa0339350:intron 35.0%
TGAAAGAAGCCAATCCAAAA+AGG - chr2_4:60487948-60487967 None:intergenic 35.0%
CTATCCCTAGATAAAGTCTT+TGG - chr2_4:60488410-60488429 None:intergenic 35.0%
CAAAGACTTTATCTAGGGAT+AGG + chr2_4:60488408-60488427 Msa0339350:CDS 35.0%
ATAGGAATCCAAAATACCCT+CGG + chr2_4:60488426-60488445 Msa0339350:CDS 35.0%
GATTGAAGACAACAACACTT+TGG + chr2_4:60488529-60488548 Msa0339350:CDS 35.0%
! GATGTCGTACATTTTCTTCA+CGG - chr2_4:60488600-60488619 None:intergenic 35.0%
! GATCAAGGTGTTAACTTTCT+TGG - chr2_4:60488627-60488646 None:intergenic 35.0%
AGAAAGTTAACACCTTGATC+AGG + chr2_4:60488627-60488646 Msa0339350:CDS 35.0%
GTATCATATCCCTTCACAAT+AGG - chr2_4:60488831-60488850 None:intergenic 35.0%
! AACTTTGCATGTTTTCTTGC+AGG + chr2_4:60488916-60488935 Msa0339350:intron 35.0%
! ACATCCAAAGCATCATAATC+AGG - chr2_4:60488978-60488997 None:intergenic 35.0%
TTGGATGTTGCAAACAAGAT+TGG + chr2_4:60488990-60489009 Msa0339350:CDS 35.0%
TGGATGTTGCAAACAAGATT+GGG + chr2_4:60488991-60489010 Msa0339350:CDS 35.0%
! GGATTATTTAAGGTAATGGC+TGG + chr2_4:60489012-60489031 Msa0339350:exon 35.0%
! TTTTATGGTGATCACCATTG+TGG + chr2_4:60489071-60489090 Msa0339350:three_prime_UTR 35.0%
ATGTGCTGTGAACATAATTG+AGG + chr2_4:60489257-60489276 Msa0339350:three_prime_UTR 35.0%
CAAGATTACCATCTCTTAAG+AGG + chr2_4:60489352-60489371 Msa0339350:three_prime_UTR 35.0%
AACAAAGTCCTCTTAAGAGA+TGG - chr2_4:60489363-60489382 None:intergenic 35.0%
!! ATGAGGTGATTGTTTTACCT+TGG - chr2_4:60489493-60489512 None:intergenic 35.0%
TAGCCTATAAATGGAACACT+TGG + chr2_4:60489597-60489616 Msa0339350:three_prime_UTR 35.0%
!!! TGTGGTTCTTTTAAAACTCC+TGG + chr2_4:60489637-60489656 Msa0339350:three_prime_UTR 35.0%
CTTCATCTTCTCCGATTCAA+TGG + chr2_4:60486874-60486893 Msa0339350:exon 40.0%
! TTTTGGTGGAGCCATTGAAT+CGG - chr2_4:60486888-60486907 None:intergenic 40.0%
GCGGGTTTAGCAAACAAATA+AGG - chr2_4:60487419-60487438 None:intergenic 40.0%
!! TTGCTGGAACATGATTTGCT+TGG - chr2_4:60487769-60487788 None:intergenic 40.0%
!! TTGCTGGAACATGATTTGCT+TGG + chr2_4:60487769-60487788 Msa0339350:intron 40.0%
TTAGAGTCATCCCATTTCTC+TGG + chr2_4:60487858-60487877 Msa0339350:intron 40.0%
GCTTCTTTCAGCTTATCTAC+CGG + chr2_4:60487958-60487977 Msa0339350:intron 40.0%
CAGTCTCACAAAACAAATGC+CGG - chr2_4:60487980-60487999 None:intergenic 40.0%
ATGTTTCCACGAGTTACGTT+GGG - chr2_4:60488062-60488081 None:intergenic 40.0%
TTGCTGCATGAAATGTGACT+AGG + chr2_4:60488242-60488261 Msa0339350:intron 40.0%
CTTCTTTGCCTTCTTCTTGA+TGG - chr2_4:60488360-60488379 None:intergenic 40.0%
AGAAGAAGGCAAAGAAGATC+AGG + chr2_4:60488363-60488382 Msa0339350:CDS 40.0%
TAGATAAAGTCTTTGGCCTG+TGG - chr2_4:60488403-60488422 None:intergenic 40.0%
ACAGGCCAAAGACTTTATCT+AGG + chr2_4:60488402-60488421 Msa0339350:CDS 40.0%
CAGGCCAAAGACTTTATCTA+GGG + chr2_4:60488403-60488422 Msa0339350:CDS 40.0%
GAAGAAAATGTACGACATCC+AGG + chr2_4:60488601-60488620 Msa0339350:CDS 40.0%
!! AGACTTGTTGAGTTGAATGC+TGG + chr2_4:60488707-60488726 Msa0339350:intron 40.0%
ATCAGGTGTCAACCTTACAT+AGG - chr2_4:60488961-60488980 None:intergenic 40.0%
GACACCTGATTATGATGCTT+TGG + chr2_4:60488971-60488990 Msa0339350:CDS 40.0%
GAGAATTAGGTAGATGGCTT+AGG - chr2_4:60489333-60489352 None:intergenic 40.0%
AAGATCTGTGCTGTCACATT+TGG + chr2_4:60489399-60489418 Msa0339350:three_prime_UTR 40.0%
AGATCTGTGCTGTCACATTT+GGG + chr2_4:60489400-60489419 Msa0339350:three_prime_UTR 40.0%
! TGAGGTGATCGTTTTACCTT+TGG - chr2_4:60489435-60489454 None:intergenic 40.0%
CCTACTTGCTTAGCATTATG+AGG - chr2_4:60489453-60489472 None:intergenic 40.0%
CCTCATAATGCTAAGCAAGT+AGG + chr2_4:60489450-60489469 Msa0339350:three_prime_UTR 40.0%
ATGCTAAGCAAGTAGGCAAA+AGG + chr2_4:60489457-60489476 Msa0339350:three_prime_UTR 40.0%
CCTACTTGTTCAGCATTATG+AGG - chr2_4:60489510-60489529 None:intergenic 40.0%
CCTCATAATGCTGAACAAGT+AGG + chr2_4:60489507-60489526 Msa0339350:three_prime_UTR 40.0%
ATGCTGAACAAGTAGGCAAA+TGG + chr2_4:60489514-60489533 Msa0339350:three_prime_UTR 40.0%
AACAAGTAGGCAAATGGTCT+GGG + chr2_4:60489520-60489539 Msa0339350:three_prime_UTR 40.0%
TTACGATGTCCTTGCACATA+TGG + chr2_4:60489572-60489591 Msa0339350:three_prime_UTR 40.0%
TAGGCTAAACCATATGTGCA+AGG - chr2_4:60489584-60489603 None:intergenic 40.0%
CAGCCAAGTGTTCCATTTAT+AGG - chr2_4:60489603-60489622 None:intergenic 40.0%
! GCTGAGCTAGTTTGAAAATG+TGG + chr2_4:60489619-60489638 Msa0339350:three_prime_UTR 40.0%
TACGTAGTATGACAACATCC+AGG - chr2_4:60489658-60489677 None:intergenic 40.0%
CAGAATTCAGCATGATACGT+AGG - chr2_4:60489748-60489767 None:intergenic 40.0%
CTACGTATCATGCTGAATTC+TGG + chr2_4:60489746-60489765 Msa0339350:three_prime_UTR 40.0%
TCACGCAGGTTTCACATCTA+AGG + chr2_4:60486847-60486866 Msa0339350:five_prime_UTR 45.0%
CACGCAGGTTTCACATCTAA+GGG + chr2_4:60486848-60486867 Msa0339350:five_prime_UTR 45.0%
ACGCAGGTTTCACATCTAAG+GGG + chr2_4:60486849-60486868 Msa0339350:five_prime_UTR 45.0%
GATTCAATGGCTCCACCAAA+AGG + chr2_4:60486887-60486906 Msa0339350:exon 45.0%
TGTTTCCACGAGTTACGTTG+GGG - chr2_4:60488061-60488080 None:intergenic 45.0%
GATGTTTCCACGAGTTACGT+TGG - chr2_4:60488063-60488082 None:intergenic 45.0%
TGCAAAGAAGACCGATCCAA+AGG + chr2_4:60488292-60488311 Msa0339350:CDS 45.0%
GGACAACTGTTACATTCCAC+AGG + chr2_4:60488384-60488403 Msa0339350:CDS 45.0%
!!! GCACTAATCCGAGGGTATTT+TGG - chr2_4:60488437-60488456 None:intergenic 45.0%
TTGGTGTAGCACTAATCCGA+GGG - chr2_4:60488445-60488464 None:intergenic 45.0%
!! ATTGCTGACTCAGTTGTGAG+CGG - chr2_4:60488503-60488522 None:intergenic 45.0%
AGGTGTTAACTTTCTTGGCC+TGG - chr2_4:60488622-60488641 None:intergenic 45.0%
CCTAAAGGCTTACCTGATCA+AGG - chr2_4:60488642-60488661 None:intergenic 45.0%
CCTTGATCAGGTAAGCCTTT+AGG + chr2_4:60488639-60488658 Msa0339350:intron 45.0%
ATGGCACACTGTTATGCTCA+CGG + chr2_4:60488803-60488822 Msa0339350:intron 45.0%
CAACCTTACATAGGCCTTCT+TGG - chr2_4:60488952-60488971 None:intergenic 45.0%
GCGTCAAAATGTCTCCACAA+TGG - chr2_4:60489088-60489107 None:intergenic 45.0%
! TTAGGCTTAGGCTGAGTTGA+TGG - chr2_4:60489309-60489328 None:intergenic 45.0%
TAGGTAGATGGCTTAGGCTT+AGG - chr2_4:60489327-60489346 None:intergenic 45.0%
GAACAAGTAGGCAAATGGTC+TGG + chr2_4:60489519-60489538 Msa0339350:three_prime_UTR 45.0%
ACAAGTAGGCAAATGGTCTG+GGG + chr2_4:60489521-60489540 Msa0339350:three_prime_UTR 45.0%
AAACCTGCGTGAAACCTGTG+TGG - chr2_4:60486839-60486858 None:intergenic 50.0%
TCACTGCTTGCACGAGTTTC+TGG + chr2_4:60487020-60487039 Msa0339350:intron 50.0%
GTTTCCACGAGTTACGTTGG+GGG - chr2_4:60488060-60488079 None:intergenic 50.0%
CACTGCTTTCGCAGCTTTCA+AGG - chr2_4:60488324-60488343 None:intergenic 50.0%
GCTGCGAAAGCAGTGAAATC+AGG + chr2_4:60488329-60488348 Msa0339350:CDS 50.0%
! AGGCACTGCCATCAAGAAGA+AGG + chr2_4:60488349-60488368 Msa0339350:CDS 50.0%
! CTTGGTGTAGCACTAATCCG+AGG - chr2_4:60488446-60488465 None:intergenic 50.0%
!! CTCGGATTAGTGCTACACCA+AGG + chr2_4:60488444-60488463 Msa0339350:CDS 50.0%
TGGCACACTGTTATGCTCAC+GGG + chr2_4:60488804-60488823 Msa0339350:intron 50.0%
! ATGTTTTCTTGCAGGCCCGA+TGG + chr2_4:60488924-60488943 Msa0339350:intron 50.0%
!! ATAGGCCTTCTTGGTGCCAT+CGG - chr2_4:60488943-60488962 None:intergenic 50.0%
GCACCAAGAAGGCCTATGTA+AGG + chr2_4:60488946-60488965 Msa0339350:CDS 50.0%
GATGGCTTAGGCTTAGGCTT+AGG - chr2_4:60489321-60489340 None:intergenic 50.0%
GCAAGTAGGCAAAAGGTCTG+CGG + chr2_4:60489464-60489483 Msa0339350:three_prime_UTR 50.0%
CAAAAGGTCTGCGGTAACCA+AGG + chr2_4:60489473-60489492 Msa0339350:three_prime_UTR 50.0%
! CAAATGGTCTGGGGTAACTG+AGG + chr2_4:60489530-60489549 Msa0339350:three_prime_UTR 50.0%
!! GTAACTGAGGTTGGTGGTCA+AGG + chr2_4:60489543-60489562 Msa0339350:three_prime_UTR 50.0%
! TGTGTGGTGGAAGCAGACAG+TGG - chr2_4:60486823-60486842 None:intergenic 55.0%
ACTGTCTGCTTCCACCACAC+AGG + chr2_4:60486822-60486841 Msa0339350:five_prime_UTR 55.0%
AGTTCCCCCAACGTAACTCG+TGG + chr2_4:60488053-60488072 Msa0339350:intron 55.0%
! GAAGACCGATCCAAAGGCAC+AGG + chr2_4:60488298-60488317 Msa0339350:CDS 55.0%
CAAGGCCTGTGCCTTTGGAT+CGG - chr2_4:60488306-60488325 None:intergenic 55.0%
GCTTTCAAGGCCTGTGCCTT+TGG - chr2_4:60488311-60488330 None:intergenic 55.0%
CTCACGGGCCCTATTGTGAA+GGG + chr2_4:60488819-60488838 Msa0339350:intron 55.0%
!! TAGGCCTTCTTGGTGCCATC+GGG - chr2_4:60488942-60488961 None:intergenic 55.0%
! TGGTCTGGGGTAACTGAGGT+TGG + chr2_4:60489534-60489553 Msa0339350:three_prime_UTR 55.0%
! TCTGGGGTAACTGAGGTTGG+TGG + chr2_4:60489537-60489556 Msa0339350:three_prime_UTR 55.0%
CCTGCGTGAAACCTGTGTGG+TGG - chr2_4:60486836-60486855 None:intergenic 60.0%
CCACCACACAGGTTTCACGC+AGG + chr2_4:60486833-60486852 Msa0339350:five_prime_UTR 60.0%
GCTCACGGGCCCTATTGTGA+AGG + chr2_4:60488818-60488837 Msa0339350:intron 60.0%
CAGGCCCGATGGCACCAAGA+AGG + chr2_4:60488935-60488954 Msa0339350:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 60486816 60489770 60486816 ID=Msa0339350;Name=Msa0339350
chr2_4 mRNA 60486816 60489770 60486816 ID=Msa0339350-mRNA-1;Parent=Msa0339350;Name=Msa0339350-mRNA-1;_AED=0.01;_eAED=0.01;_QI=77|1|1|1|1|1|3|748|154
chr2_4 exon 60486816 60486908 60486816 ID=Msa0339350-mRNA-1:exon:18770;Parent=Msa0339350-mRNA-1
chr2_4 exon 60488285 60488648 60488285 ID=Msa0339350-mRNA-1:exon:18771;Parent=Msa0339350-mRNA-1
chr2_4 exon 60488938 60489770 60488938 ID=Msa0339350-mRNA-1:exon:18772;Parent=Msa0339350-mRNA-1
chr2_4 five_prime_UTR 60486816 60486892 60486816 ID=Msa0339350-mRNA-1:five_prime_utr;Parent=Msa0339350-mRNA-1
chr2_4 CDS 60486893 60486908 60486893 ID=Msa0339350-mRNA-1:cds;Parent=Msa0339350-mRNA-1
chr2_4 CDS 60488285 60488648 60488285 ID=Msa0339350-mRNA-1:cds;Parent=Msa0339350-mRNA-1
chr2_4 CDS 60488938 60489022 60488938 ID=Msa0339350-mRNA-1:cds;Parent=Msa0339350-mRNA-1
chr2_4 three_prime_UTR 60489023 60489770 60489023 ID=Msa0339350-mRNA-1:three_prime_utr;Parent=Msa0339350-mRNA-1
Gene Sequence

>Msa0339350

ATGGCTCCACCAAAAGGTGATGGTGCAAAGAAGACCGATCCAAAGGCACAGGCCTTGAAAGCTGCGAAAGCAGTGAAATCAGGCACTGCCATCAAGAAGAAGGCAAAGAAGATCAGGACAACTGTTACATTCCACAGGCCAAAGACTTTATCTAGGGATAGGAATCCAAAATACCCTCGGATTAGTGCTACACCAAGGAACAAACTTGATCATTATCAGATTTTGAAGTATCCGCTCACAACTGAGTCAGCAATGAAGAAGATTGAAGACAACAACACTTTGGTTTTCATTGTTGACTTGCGAGCTGACAAAAAGAAAATCAAGGATGCCGTGAAGAAAATGTACGACATCCAGGCCAAGAAAGTTAACACCTTGATCAGGCCCGATGGCACCAAGAAGGCCTATGTAAGGTTGACACCTGATTATGATGCTTTGGATGTTGCAAACAAGATTGGGATTATTTAA
Protein sequence

>Msa0339350

MAPPKGDGAKKTDPKAQALKAAKAVKSGTAIKKKAKKIRTTVTFHRPKTLSRDRNPKYPRISATPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQAKKVNTLIRPDGTKKAYVRLTPDYDALDVANKIGII*