AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0339730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0339730 MtrunA17_Chr2g0328431 96.341 656 24 0 1 656 1 656 0.0 1317
Msa0339730 MtrunA17_Chr2g0328411 92.202 654 51 0 1 654 1 654 0.0 1213
Msa0339730 MtrunA17_Chr2g0328401 79.717 636 128 1 1 635 1 636 0.0 1042
Msa0339730 MtrunA17_Chr4g0031741 53.613 692 245 13 1 656 1 652 0.0 675
Msa0339730 MtrunA17_Chr7g0238891 44.088 685 303 14 44 656 66 742 0.0 536
Msa0339730 MtrunA17_Chr2g0328391 42.793 666 348 13 4 656 26 671 3.49e-171 504
Msa0339730 MtrunA17_Chr4g0031701 45.556 630 311 12 38 656 20 628 6.21e-171 502
Msa0339730 MtrunA17_Chr4g0031711 43.239 673 312 18 38 656 79 735 3.18e-168 498
Msa0339730 MtrunA17_Chr4g0031721 52.878 469 202 6 200 656 225 686 1.50e-167 495
Msa0339730 MtrunA17_Chr4g0031721 43.662 71 38 1 29 99 13 81 9.89e-11 65.1
Msa0339730 MtrunA17_Chr4g0031731 45.238 630 313 12 38 656 20 628 1.85e-167 493
Msa0339730 MtrunA17_Chr2g0328381 46.189 433 220 6 229 656 219 643 7.57e-132 402
Msa0339730 MtrunA17_Chr2g0328341 46.651 433 217 7 229 656 217 640 4.16e-131 400
Msa0339730 MtrunA17_Chr2g0328361 46.825 378 169 6 283 656 268 617 2.51e-114 355
Msa0339730 MtrunA17_Chr2g0328371 51.562 256 119 2 356 610 1 252 2.08e-92 286
Msa0339730 MtrunA17_Chr5g0447691 32.888 374 243 7 285 653 173 543 8.26e-57 201
Msa0339730 MtrunA17_Chr2g0290241 30.133 375 255 6 284 653 52 424 1.27e-56 197
Msa0339730 MtrunA17_Chr2g0319091 31.714 391 261 5 267 653 190 578 1.67e-55 198
Msa0339730 MtrunA17_Chr4g0076091 28.780 410 274 8 259 654 138 543 2.09e-52 189
Msa0339730 MtrunA17_Chr7g0224271 29.337 392 245 5 282 653 293 672 5.72e-51 187
Msa0339730 MtrunA17_Chr1g0159461 28.726 369 257 5 289 653 225 591 2.59e-50 184
Msa0339730 MtrunA17_Chr8g0376381 31.467 375 237 9 283 653 163 521 1.09e-48 179
Msa0339730 MtrunA17_Chr3g0101141 29.491 373 257 5 285 653 171 541 1.70e-48 178
Msa0339730 MtrunA17_Chr5g0445331 30.159 378 252 8 285 653 157 531 2.75e-48 177
Msa0339730 MtrunA17_Chr4g0057891 28.462 390 251 7 281 653 141 519 7.63e-47 173
Msa0339730 MtrunA17_Chr3g0125811 27.913 369 260 5 289 653 203 569 2.23e-46 173
Msa0339730 MtrunA17_Chr8g0357601 29.156 391 248 6 283 656 176 554 2.91e-46 172
Msa0339730 MtrunA17_Chr2g0306641 30.479 397 252 11 269 653 102 486 1.29e-45 169
Msa0339730 MtrunA17_Chr3g0110971 29.381 388 243 9 283 653 213 586 5.12e-45 169
Msa0339730 MtrunA17_Chr7g0245601 29.428 367 242 7 292 655 441 793 4.81e-43 166
Msa0339730 MtrunA17_Chr4g0038851 26.230 427 247 7 289 653 48 468 7.24e-40 152
Msa0339730 MtrunA17_Chr3g0085681 26.859 417 245 7 290 653 26 435 1.31e-39 151
Msa0339730 MtrunA17_Chr4g0038761 27.926 376 242 8 292 656 87 444 5.43e-39 149
Msa0339730 MtrunA17_Chr2g0294461 28.645 391 247 8 284 653 205 584 5.31e-37 146
Msa0339730 MtrunA17_Chr4g0057291 23.969 388 270 9 283 655 286 663 2.69e-35 142
Msa0339730 MtrunA17_Chr1g0200401 26.515 396 246 12 289 653 77 458 8.39e-32 129
Msa0339730 MtrunA17_Chr4g0039661 25.464 377 260 7 286 653 190 554 1.03e-31 130
Msa0339730 MtrunA17_Chr2g0294471 26.753 385 259 8 284 653 199 575 2.52e-31 129
Msa0339730 MtrunA17_Chr7g0270031 26.842 380 258 9 284 653 184 553 1.10e-29 124
Msa0339730 MtrunA17_Chr1g0183641 26.247 381 250 13 283 653 117 476 1.95e-29 122
Msa0339730 MtrunA17_Chr5g0401131 25.000 384 265 8 289 655 114 491 5.03e-29 121
Msa0339730 MtrunA17_Chr7g0236851 28.938 273 181 6 383 653 395 656 4.58e-26 113
Msa0339730 MtrunA17_Chr5g0424131 25.627 359 237 10 316 654 151 499 2.37e-25 110
Msa0339730 MtrunA17_Chr5g0396491 31.579 209 133 2 289 493 56 258 6.88e-25 108
Msa0339730 MtrunA17_Chr5g0396491 29.530 149 105 0 505 653 329 477 3.61e-16 81.6
Msa0339730 MtrunA17_Chr3g0085361 25.071 351 206 5 352 653 7 349 3.04e-24 104
Msa0339730 MtrunA17_Chr3g0114841 26.685 371 231 13 308 653 145 499 5.18e-24 106
Msa0339730 MtrunA17_Chr8g0383981 24.538 379 246 16 294 656 144 498 6.44e-24 105
Msa0339730 MtrunA17_Chr1g0194621 22.938 388 270 9 284 653 141 517 1.76e-23 104
Msa0339730 MtrunA17_Chr6g0468561 26.087 345 226 10 324 653 331 661 4.78e-23 104
Msa0339730 MtrunA17_Chr5g0401421 21.780 427 280 10 279 656 27 448 4.94e-22 99.8
Msa0339730 MtrunA17_Chr2g0329591 25.824 364 220 10 289 623 74 416 3.64e-20 94.0
Msa0339730 MtrunA17_Chr4g0053901 21.635 416 274 8 289 656 59 470 3.94e-20 94.0
Msa0339730 MtrunA17_Chr7g0230731 23.861 373 257 10 290 653 373 727 2.62e-18 89.4
Msa0339730 MtrunA17_Chr7g0230771 23.181 371 262 9 290 653 376 730 6.85e-18 88.2
Msa0339730 MtrunA17_Chr4g0012071 21.939 392 274 10 275 653 178 550 1.80e-17 86.3
Msa0339730 MtrunA17_Chr8g0344101 24.750 400 243 16 289 653 223 599 1.30e-15 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0339730 AT2G37650.1 43.256 645 320 12 50 655 77 714 7.34e-167 495
Msa0339730 AT2G37650.2 43.256 645 320 12 50 655 65 702 1.17e-166 494
Msa0339730 AT2G29060.2 43.038 632 303 14 50 655 56 656 1.28e-164 487
Msa0339730 AT2G29060.1 42.590 641 330 12 50 655 56 693 5.46e-162 481
Msa0339730 AT5G59450.1 49.263 475 224 7 193 656 132 600 5.11e-158 468
Msa0339730 AT1G07530.1 49.573 468 221 5 201 656 303 767 1.28e-155 468
Msa0339730 AT3G46600.3 50.557 449 199 5 216 656 116 549 1.46e-149 444
Msa0339730 AT3G46600.1 50.557 449 199 5 216 656 148 581 1.79e-149 446
Msa0339730 AT3G46600.2 55.405 370 163 1 289 656 4 373 3.20e-147 432
Msa0339730 AT2G29065.1 41.707 621 323 12 51 656 34 630 1.37e-143 432
Msa0339730 AT1G07520.2 45.021 482 247 6 185 656 58 531 1.25e-136 410
Msa0339730 AT1G07520.4 45.021 482 247 6 185 656 58 531 1.25e-136 410
Msa0339730 AT1G07520.1 38.975 644 339 14 52 656 67 695 3.60e-136 415
Msa0339730 AT1G07520.3 38.975 644 339 14 52 656 68 696 3.74e-136 415
Msa0339730 AT1G50600.3 34.759 374 236 6 285 653 155 525 5.82e-60 210
Msa0339730 AT1G50600.1 34.759 374 236 6 285 653 226 596 2.13e-59 210
Msa0339730 AT1G50600.2 34.759 374 236 6 285 653 230 600 3.37e-59 209
Msa0339730 AT5G48150.1 32.292 384 251 7 276 653 109 489 2.71e-55 196
Msa0339730 AT5G48150.4 32.292 384 251 7 276 653 109 489 2.71e-55 196
Msa0339730 AT5G48150.2 32.292 384 251 7 276 653 109 489 2.71e-55 196
Msa0339730 AT5G48150.3 32.292 384 251 7 276 653 109 489 2.71e-55 196
Msa0339730 AT2G04890.1 30.027 373 248 6 285 653 49 412 1.99e-50 181
Msa0339730 AT1G21450.1 28.455 369 259 4 289 653 225 592 1.32e-47 177
Msa0339730 AT4G17230.1 30.053 376 257 5 284 655 153 526 3.84e-47 174
Msa0339730 AT4G17230.2 30.053 376 257 5 284 655 153 526 3.84e-47 174
Msa0339730 AT1G14920.1 30.400 375 245 9 283 653 166 528 3.12e-45 169
Msa0339730 AT3G54220.1 30.295 373 234 9 292 655 296 651 4.86e-44 168
Msa0339730 AT3G03450.1 29.306 389 236 9 283 653 177 544 1.25e-43 165
Msa0339730 AT2G01570.1 28.533 375 252 9 283 653 218 580 7.49e-42 160
Msa0339730 AT1G66350.1 29.894 378 237 10 283 653 149 505 7.51e-42 159
Msa0339730 AT5G66770.1 28.686 373 255 7 286 653 217 583 2.49e-41 159
Msa0339730 AT5G17490.1 29.365 378 244 9 283 653 154 515 1.03e-40 156
Msa0339730 AT5G41920.1 27.013 385 253 7 279 653 33 399 3.97e-38 146
Msa0339730 AT3G50650.1 26.133 375 260 7 290 653 173 541 4.21e-36 143
Msa0339730 AT1G63100.1 25.723 346 240 6 285 622 248 584 7.29e-33 134
Msa0339730 AT1G63100.2 25.723 346 240 6 285 622 273 609 8.99e-33 134
Msa0339730 AT4G37650.1 24.566 403 252 11 288 655 145 530 3.92e-32 131
Msa0339730 AT5G52510.1 26.190 378 254 9 292 653 271 639 1.85e-30 127
Msa0339730 AT3G49950.1 27.041 392 249 11 289 655 30 409 3.85e-28 117
Msa0339730 AT4G08250.1 28.022 364 235 13 305 653 125 476 9.09e-28 117
Msa0339730 AT1G50420.1 28.761 226 146 4 286 502 54 273 1.46e-23 105
Msa0339730 AT1G50420.1 28.859 149 106 0 505 653 330 478 2.59e-15 79.3
Msa0339730 AT1G55580.1 23.256 387 231 8 318 653 73 444 8.34e-22 99.4
Msa0339730 AT3G13840.1 24.289 387 246 14 289 653 148 509 2.04e-19 92.4
Msa0339730 AT4G00150.1 21.728 382 264 9 280 653 199 553 2.46e-13 73.6

Find 134 sgRNAs with CRISPR-Local

Find 318 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAGTTCCATTGTGAAAA+TGG 0.175227 2_4:-60998029 None:intergenic
CTCACAAGCTATAACATTTA+TGG 0.192701 2_4:-60999231 None:intergenic
CATCGACTACAAAATCTTTA+TGG 0.219672 2_4:-60999385 None:intergenic
CCATCTTCACCTTCTTCTTC+TGG 0.264392 2_4:+60997598 Msa0339730:CDS
AGCGTTCATGTTATTGATTT+TGG 0.271020 2_4:+60998678 Msa0339730:CDS
CCTGACCTGCCACTGCTTGT+AGG 0.274499 2_4:-60999282 None:intergenic
CCTCCAAAGCTTCGAATTAC+AGG 0.281535 2_4:+60998762 Msa0339730:CDS
TATGTATCTTAGGATGTTAT+TGG 0.287625 2_4:-60997662 None:intergenic
GTTACTTGGTTTACTGAGTT+TGG 0.288731 2_4:-60997550 None:intergenic
AGGCAACACTCTTCGCCTTT+TGG 0.309946 2_4:+60998447 Msa0339730:CDS
ACTCCATGGAAGAAGACTTT+TGG 0.314241 2_4:-60999056 None:intergenic
GGATGTCATTTATGTATCTT+AGG 0.317485 2_4:-60997672 None:intergenic
ACAACTTCATTGCACAGAAA+TGG 0.347339 2_4:+60998886 Msa0339730:CDS
TTTGAATGCTTTATCAGCTT+GGG 0.351490 2_4:+60999448 Msa0339730:CDS
AAGTCCTTCTACGATGTTCT+TGG 0.359023 2_4:+60997769 Msa0339730:CDS
GCAGGAATGTTTAAGACTTC+AGG 0.359559 2_4:+60997738 Msa0339730:CDS
ATTCCTGTAATTCGAAGCTT+TGG 0.359837 2_4:-60998765 None:intergenic
GCATCACATAATGTTGATTC+AGG 0.366614 2_4:+60998273 Msa0339730:CDS
TTAGCTCATTACTTTGCTAA+TGG 0.370579 2_4:+60998486 Msa0339730:CDS
TAAAGGACATTGTAGTTGTA+TGG 0.378502 2_4:-60997941 None:intergenic
CATTTGGTGAAGGCTGGTTA+TGG 0.381462 2_4:-60997792 None:intergenic
AAATCTTGCAGAAAAGTGTT+TGG 0.386250 2_4:-60997520 None:intergenic
TCTTCTGGCACAAGCTCAAA+TGG 0.386431 2_4:+60997613 Msa0339730:CDS
AAGATGTGTTGAAGCTGATC+AGG 0.390657 2_4:+60999024 Msa0339730:CDS
CCTCTTGATGTGGCAACTGC+TGG 0.394427 2_4:+60998549 Msa0339730:CDS
TTCTGACGAGGCACGGCCAC+TGG 0.399566 2_4:-60998716 None:intergenic
TCAGTAAACCAAGTAACAAA+TGG 0.401437 2_4:+60997556 Msa0339730:CDS
CCTTGTGTCTCGATGACAAC+TGG 0.405616 2_4:-60998000 None:intergenic
ATTCAAACCCATTTGTTACT+TGG 0.405951 2_4:-60997564 None:intergenic
CCTACAAGCAGTGGCAGGTC+AGG 0.408410 2_4:+60999282 Msa0339730:CDS
AGCTTCAACACATCTTCTCT+TGG 0.409629 2_4:-60999017 None:intergenic
GAGCTGGGTTCAAGCAAATC+AGG 0.413441 2_4:+60999312 Msa0339730:CDS
ATGCTTGAGAGAGGGTTGTT+TGG 0.418474 2_4:+60999200 Msa0339730:CDS
GTTTCCTCCACCCTTTCTGC+AGG 0.420825 2_4:-60998813 None:intergenic
CAACATTATGTGATGCTTGT+TGG 0.426130 2_4:-60998266 None:intergenic
CAACTTCATTGCACAGAAAT+GGG 0.427023 2_4:+60998887 Msa0339730:CDS
TGCCACTGCTTGTAGGTCTC+AGG 0.429715 2_4:-60999275 None:intergenic
ATCGTTGTTTGATATGTTTG+AGG 0.437931 2_4:+60999145 Msa0339730:CDS
ATGTTATTGGAATATCTTGT+CGG 0.439317 2_4:-60997649 None:intergenic
TTCAATGCCACAGTTTCATC+AGG 0.440053 2_4:-60998990 None:intergenic
TCTGGGTCAGTGCTATCATT+TGG 0.448392 2_4:-60997808 None:intergenic
GCGCGATCGAAATTTCCATC+TGG 0.453753 2_4:-60997826 None:intergenic
TGCTATCATTTGGTGAAGGC+TGG 0.460261 2_4:-60997798 None:intergenic
ATGCCGCGGCAGTGGATTTG+TGG 0.465356 2_4:+60998349 Msa0339730:CDS
CATGGATGAAGAAGATGACT+TGG 0.465816 2_4:+60997696 Msa0339730:CDS
GCAGAAAGGGTGGAGGAAAC+CGG 0.469253 2_4:+60998816 Msa0339730:CDS
AAGCTCAAATGGACAATCCT+TGG 0.473031 2_4:+60997624 Msa0339730:CDS
ATGGCCAAGAACATCGTAGA+AGG 0.479055 2_4:-60997773 None:intergenic
TTCACAATGGAACTTGGAGT+TGG 0.481843 2_4:+60998034 Msa0339730:CDS
AGTACCTCATCTAAAATCTC+TGG 0.483079 2_4:-60998210 None:intergenic
AGCTTGTGAGGGTGCAGAAA+GGG 0.483774 2_4:+60999244 Msa0339730:CDS
TAGTCGATGTGGATGGTAAG+TGG 0.492425 2_4:+60999399 Msa0339730:CDS
GGAATTGAGCTTCCTCGGCC+AGG 0.495711 2_4:+60998783 Msa0339730:CDS
CGCTGGAGTCCATCACCAAA+AGG 0.496232 2_4:-60998462 None:intergenic
AAAACTTCTTCCACCGTCAC+TGG 0.496341 2_4:-60998069 None:intergenic
CGAAGCTTTGGAGGACCACC+AGG 0.497005 2_4:-60998753 None:intergenic
CCTGTAATTCGAAGCTTTGG+AGG 0.497546 2_4:-60998762 None:intergenic
AATCCAAAAGTCTTCTTCCA+TGG 0.502095 2_4:+60999053 Msa0339730:CDS
TAATGGTCTTGAGATAAGAT+TGG 0.503318 2_4:+60998503 Msa0339730:CDS
CTTTCTGCAGGTCTAAATCC+TGG 0.504296 2_4:-60998801 None:intergenic
GACAAACAATGCCGCGGCAG+TGG 0.508260 2_4:+60998341 Msa0339730:CDS
GTGCTTCCTTAAACCGAGTC+GGG 0.509273 2_4:-60999109 None:intergenic
TTCTTCCATGGAGTTGCCAA+TGG 0.510258 2_4:+60999065 Msa0339730:CDS
AACGAAAACAATCGTGAAGA+AGG 0.511353 2_4:+60999358 Msa0339730:CDS
ATGAGCTACTCAAACAGATT+AGG 0.511922 2_4:+60998427 Msa0339730:CDS
CAGTAAACCAAGTAACAAAT+GGG 0.514266 2_4:+60997557 Msa0339730:CDS
CTTTACATCCTCGAGGCAGA+TGG 0.514553 2_4:-60998913 None:intergenic
TTACAGGAATTGAGCTTCCT+CGG 0.515630 2_4:+60998778 Msa0339730:CDS
CAGGTCAGGAATAAGAGAGC+TGG 0.517229 2_4:+60999296 Msa0339730:CDS
CGCGATCGAAATTTCCATCT+GGG 0.519839 2_4:-60997825 None:intergenic
AGGATTTAGACCTGCAGAAA+GGG 0.522516 2_4:+60998803 Msa0339730:CDS
TCGAGGATGTAAAGATAGAC+AGG 0.523675 2_4:+60998922 Msa0339730:CDS
GGAATATCTTGTCGGTTCCA+AGG 0.526658 2_4:-60997641 None:intergenic
GAGCTTGTGCCAGAAGAAGA+AGG 0.528577 2_4:-60997607 None:intergenic
AAACATTCCTGCAGCATGCA+AGG 0.535746 2_4:-60997727 None:intergenic
CTGTGGCGAGCTACGATCAA+AGG 0.536487 2_4:+60998397 Msa0339730:CDS
GTAGTTTGCTAGACGCCGTC+CGG 0.538457 2_4:-60998835 None:intergenic
CAGGATTTAGACCTGCAGAA+AGG 0.540009 2_4:+60998802 Msa0339730:CDS
AACTTCCATTGGCAACTCCA+TGG 0.540581 2_4:-60999070 None:intergenic
GCAATGAAGTTGTATTCAAA+TGG 0.550757 2_4:-60998876 None:intergenic
AGTCGATGTGGATGGTAAGT+GGG 0.556259 2_4:+60999400 Msa0339730:CDS
CGGTGCGCTATAACTTCCAT+TGG 0.556645 2_4:-60999081 None:intergenic
TAATGTTGATTCAGGAGGAA+AGG 0.556753 2_4:+60998281 Msa0339730:CDS
TTGTGCACATTGATTTAGCA+TGG 0.558170 2_4:-60998373 None:intergenic
CCAGAAGAAGAAGGTGAAGA+TGG 0.558793 2_4:-60997598 None:intergenic
ATGCCACAGTTTCATCAGGA+AGG 0.560068 2_4:-60998986 None:intergenic
CCAGCAGTTGCCACATCAAG+AGG 0.562934 2_4:-60998549 None:intergenic
TGAGACCTACAAGCAGTGGC+AGG 0.569446 2_4:+60999277 Msa0339730:CDS
TAACTCATATGAATCTAACT+TGG 0.569816 2_4:+60997888 Msa0339730:CDS
ATACATAAATGACATCCTCA+TGG 0.570653 2_4:+60997678 Msa0339730:CDS
CATAAATGTTATAGCTTGTG+AGG 0.573605 2_4:+60999232 Msa0339730:CDS
AGGTCAGGAATAAGAGAGCT+GGG 0.580907 2_4:+60999297 Msa0339730:CDS
ACATCAAGAGGCTGATATGA+TGG 0.581066 2_4:-60998537 None:intergenic
TGTAATGAACAAGAGGGAAG+AGG 0.582616 2_4:+60998147 Msa0339730:CDS
TATCAAACAACGATGAGAAG+TGG 0.582630 2_4:-60999136 None:intergenic
CACTCTCTGCAAAGGAGACG+CGG 0.582762 2_4:-60998604 None:intergenic
CAGAGATCGATGCTTGAGAG+AGG 0.587984 2_4:+60999191 Msa0339730:CDS
CAAAGTAATGAGCTAAACGC+TGG 0.593615 2_4:-60998479 None:intergenic
TGATACTTGTAATGAACAAG+AGG 0.595387 2_4:+60998140 Msa0339730:CDS
GAGGTACTGCTTTATCAGAA+AGG 0.596446 2_4:+60998225 Msa0339730:CDS
ATCATATCAGCCTCTTGATG+TGG 0.597759 2_4:+60998539 Msa0339730:CDS
GTATTTGTCACTCTCTGCAA+AGG 0.601498 2_4:-60998612 None:intergenic
ATTTAGACCTGCAGAAAGGG+TGG 0.601556 2_4:+60998806 Msa0339730:CDS
AGGGTGCAGAAAGGGTTGAA+AGG 0.602354 2_4:+60999252 Msa0339730:CDS
GATAGTCTTCTGACGAGGCA+CGG 0.602753 2_4:-60998723 None:intergenic
ACCTCATCTAAAATCTCTGG+TGG 0.604084 2_4:-60998207 None:intergenic
AGTGCTTCCTTAAACCGAGT+CGG 0.604179 2_4:-60999110 None:intergenic
GCAGGTCTAAATCCTGGCCG+AGG 0.605176 2_4:-60998795 None:intergenic
TAGCTTGTGAGGGTGCAGAA+AGG 0.607236 2_4:+60999243 Msa0339730:CDS
AATGATAGCACTGACCCAGA+TGG 0.609483 2_4:+60997811 Msa0339730:CDS
AAGATTCGAATTCGACGACA+TGG 0.612871 2_4:-60997915 None:intergenic
CCAGTTGTCATCGAGACACA+AGG 0.613282 2_4:+60998000 Msa0339730:CDS
TGGGTTCAAGCAAATCAGGT+TGG 0.613823 2_4:+60999316 Msa0339730:CDS
TAGACCTGCAGAAAGGGTGG+AGG 0.621359 2_4:+60998809 Msa0339730:CDS
GGCCTGAGACCTACAAGCAG+TGG 0.622095 2_4:+60999273 Msa0339730:CDS
GATACTTGTAATGAACAAGA+GGG 0.631778 2_4:+60998141 Msa0339730:CDS
TCAGTGCTATCATTTGGTGA+AGG 0.638613 2_4:-60997802 None:intergenic
TGACATTCAGCCAGTGACGG+TGG 0.640113 2_4:+60998059 Msa0339730:CDS
TCACATAATGTTGATTCAGG+AGG 0.642164 2_4:+60998276 Msa0339730:CDS
GGTCCACAAATCCACTGCCG+CGG 0.645648 2_4:-60998352 None:intergenic
GAATGACATTCAGCCAGTGA+CGG 0.650343 2_4:+60998056 Msa0339730:CDS
AGAGATCGATGCTTGAGAGA+GGG 0.659674 2_4:+60999192 Msa0339730:CDS
GAACCTTCCTGATGAAACTG+TGG 0.660816 2_4:+60998983 Msa0339730:CDS
AAATCAATGTGCACAAGCTG+TGG 0.661819 2_4:+60998380 Msa0339730:CDS
TGTCTATCTTTACATCCTCG+AGG 0.665186 2_4:-60998920 None:intergenic
AGACTATCATTAAGACCTGG+TGG 0.687440 2_4:+60998738 Msa0339730:CDS
AGTCATCTTCTTCATCCATG+AGG 0.688746 2_4:-60997693 None:intergenic
AAAGGGTGGAGGAAACCGGA+CGG 0.693036 2_4:+60998820 Msa0339730:CDS
AGAAGACTATCATTAAGACC+TGG 0.694335 2_4:+60998735 Msa0339730:CDS
ATAAATGTTATAGCTTGTGA+GGG 0.694950 2_4:+60999233 Msa0339730:CDS
ATGGGAAACCATCTGCCTCG+AGG 0.697664 2_4:+60998905 Msa0339730:CDS
ATCTACGACAAACAATGCCG+CGG 0.702968 2_4:+60998335 Msa0339730:CDS
GATCTCTGCATGTCTTCACG+AGG 0.714627 2_4:-60999176 None:intergenic
TTAATGATAGTCTTCTGACG+AGG 0.747045 2_4:-60998728 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTAGTTTTTTTCTATTAA+AGG - chr2_4:60997961-60997980 None:intergenic 15.0%
!!! TAGTAGTTTTTTTCTATTAA+AGG - chr2_4:60997961-60997980 None:intergenic 15.0%
!!! ATTGATTTTGGAATTTTCTA+TGG + chr2_4:60998690-60998709 Msa0339730:CDS 20.0%
!!! ATTGATTTTGGAATTTTCTA+TGG + chr2_4:60998690-60998709 Msa0339730:CDS 20.0%
! ATGTTATTGGAATATCTTGT+CGG - chr2_4:60997652-60997671 None:intergenic 25.0%
! TATGTATCTTAGGATGTTAT+TGG - chr2_4:60997665-60997684 None:intergenic 25.0%
! TAACTCATATGAATCTAACT+TGG + chr2_4:60997888-60997907 Msa0339730:CDS 25.0%
!!! CACTATTTTGAAAATCGTTT+TGG - chr2_4:60998646-60998665 None:intergenic 25.0%
!! ATAAATGTTATAGCTTGTGA+GGG + chr2_4:60999233-60999252 Msa0339730:CDS 25.0%
! ATGTTATTGGAATATCTTGT+CGG - chr2_4:60997652-60997671 None:intergenic 25.0%
! TATGTATCTTAGGATGTTAT+TGG - chr2_4:60997665-60997684 None:intergenic 25.0%
! TAACTCATATGAATCTAACT+TGG + chr2_4:60997888-60997907 Msa0339730:CDS 25.0%
!!! CACTATTTTGAAAATCGTTT+TGG - chr2_4:60998646-60998665 None:intergenic 25.0%
!! ATAAATGTTATAGCTTGTGA+GGG + chr2_4:60999233-60999252 Msa0339730:CDS 25.0%
!! AAATCTTGCAGAAAAGTGTT+TGG - chr2_4:60997523-60997542 None:intergenic 30.0%
TCAGTAAACCAAGTAACAAA+TGG + chr2_4:60997556-60997575 Msa0339730:CDS 30.0%
CAGTAAACCAAGTAACAAAT+GGG + chr2_4:60997557-60997576 Msa0339730:CDS 30.0%
! ATTCAAACCCATTTGTTACT+TGG - chr2_4:60997567-60997586 None:intergenic 30.0%
! GGATGTCATTTATGTATCTT+AGG - chr2_4:60997675-60997694 None:intergenic 30.0%
ATACATAAATGACATCCTCA+TGG + chr2_4:60997678-60997697 Msa0339730:CDS 30.0%
TAAAGGACATTGTAGTTGTA+TGG - chr2_4:60997944-60997963 None:intergenic 30.0%
!!! ATCTTACGACTTCTTTTTTC+TGG - chr2_4:60998108-60998127 None:intergenic 30.0%
AGAAAAAAGAAGTCGTAAGA+TGG + chr2_4:60998107-60998126 Msa0339730:CDS 30.0%
TGATACTTGTAATGAACAAG+AGG + chr2_4:60998140-60998159 Msa0339730:CDS 30.0%
GATACTTGTAATGAACAAGA+GGG + chr2_4:60998141-60998160 Msa0339730:CDS 30.0%
TTAGCTCATTACTTTGCTAA+TGG + chr2_4:60998486-60998505 Msa0339730:CDS 30.0%
! TAATGGTCTTGAGATAAGAT+TGG + chr2_4:60998503-60998522 Msa0339730:CDS 30.0%
!!! AGCGTTCATGTTATTGATTT+TGG + chr2_4:60998678-60998697 Msa0339730:CDS 30.0%
GCAATGAAGTTGTATTCAAA+TGG - chr2_4:60998879-60998898 None:intergenic 30.0%
ATCGTTGTTTGATATGTTTG+AGG + chr2_4:60999145-60999164 Msa0339730:CDS 30.0%
CTCACAAGCTATAACATTTA+TGG - chr2_4:60999234-60999253 None:intergenic 30.0%
! CATAAATGTTATAGCTTGTG+AGG + chr2_4:60999232-60999251 Msa0339730:CDS 30.0%
! CATCGACTACAAAATCTTTA+TGG - chr2_4:60999388-60999407 None:intergenic 30.0%
! TAAAGATTTTGTAGTCGATG+TGG + chr2_4:60999388-60999407 Msa0339730:CDS 30.0%
! TTTTGAATGCTTTATCAGCT+TGG + chr2_4:60999447-60999466 Msa0339730:CDS 30.0%
TTTGAATGCTTTATCAGCTT+GGG + chr2_4:60999448-60999467 Msa0339730:CDS 30.0%
!! AAATCTTGCAGAAAAGTGTT+TGG - chr2_4:60997523-60997542 None:intergenic 30.0%
TCAGTAAACCAAGTAACAAA+TGG + chr2_4:60997556-60997575 Msa0339730:CDS 30.0%
CAGTAAACCAAGTAACAAAT+GGG + chr2_4:60997557-60997576 Msa0339730:CDS 30.0%
! ATTCAAACCCATTTGTTACT+TGG - chr2_4:60997567-60997586 None:intergenic 30.0%
! GGATGTCATTTATGTATCTT+AGG - chr2_4:60997675-60997694 None:intergenic 30.0%
ATACATAAATGACATCCTCA+TGG + chr2_4:60997678-60997697 Msa0339730:CDS 30.0%
TAAAGGACATTGTAGTTGTA+TGG - chr2_4:60997944-60997963 None:intergenic 30.0%
!!! ATCTTACGACTTCTTTTTTC+TGG - chr2_4:60998108-60998127 None:intergenic 30.0%
AGAAAAAAGAAGTCGTAAGA+TGG + chr2_4:60998107-60998126 Msa0339730:CDS 30.0%
TGATACTTGTAATGAACAAG+AGG + chr2_4:60998140-60998159 Msa0339730:CDS 30.0%
GATACTTGTAATGAACAAGA+GGG + chr2_4:60998141-60998160 Msa0339730:CDS 30.0%
TTAGCTCATTACTTTGCTAA+TGG + chr2_4:60998486-60998505 Msa0339730:CDS 30.0%
! TAATGGTCTTGAGATAAGAT+TGG + chr2_4:60998503-60998522 Msa0339730:CDS 30.0%
!!! AGCGTTCATGTTATTGATTT+TGG + chr2_4:60998678-60998697 Msa0339730:CDS 30.0%
GCAATGAAGTTGTATTCAAA+TGG - chr2_4:60998879-60998898 None:intergenic 30.0%
ATCGTTGTTTGATATGTTTG+AGG + chr2_4:60999145-60999164 Msa0339730:CDS 30.0%
CTCACAAGCTATAACATTTA+TGG - chr2_4:60999234-60999253 None:intergenic 30.0%
! CATAAATGTTATAGCTTGTG+AGG + chr2_4:60999232-60999251 Msa0339730:CDS 30.0%
! CATCGACTACAAAATCTTTA+TGG - chr2_4:60999388-60999407 None:intergenic 30.0%
! TAAAGATTTTGTAGTCGATG+TGG + chr2_4:60999388-60999407 Msa0339730:CDS 30.0%
! TTTTGAATGCTTTATCAGCT+TGG + chr2_4:60999447-60999466 Msa0339730:CDS 30.0%
TTTGAATGCTTTATCAGCTT+GGG + chr2_4:60999448-60999467 Msa0339730:CDS 30.0%
GTTACTTGGTTTACTGAGTT+TGG - chr2_4:60997553-60997572 None:intergenic 35.0%
! ACCATTTTCACAATGGAACT+TGG + chr2_4:60998028-60998047 Msa0339730:CDS 35.0%
TCCAAGTTCCATTGTGAAAA+TGG - chr2_4:60998032-60998051 None:intergenic 35.0%
!!! CTTTTTTCTGGTTCTGTTGA+AGG - chr2_4:60998096-60998115 None:intergenic 35.0%
! AGTACCTCATCTAAAATCTC+TGG - chr2_4:60998213-60998232 None:intergenic 35.0%
CAACATTATGTGATGCTTGT+TGG - chr2_4:60998269-60998288 None:intergenic 35.0%
!! GCATCACATAATGTTGATTC+AGG + chr2_4:60998273-60998292 Msa0339730:CDS 35.0%
!! TCACATAATGTTGATTCAGG+AGG + chr2_4:60998276-60998295 Msa0339730:CDS 35.0%
!! TAATGTTGATTCAGGAGGAA+AGG + chr2_4:60998281-60998300 Msa0339730:CDS 35.0%
!! TTGTGCACATTGATTTAGCA+TGG - chr2_4:60998376-60998395 None:intergenic 35.0%
ATGAGCTACTCAAACAGATT+AGG + chr2_4:60998427-60998446 Msa0339730:CDS 35.0%
! GAATTTTCTATGGTTTCCAG+TGG + chr2_4:60998700-60998719 Msa0339730:CDS 35.0%
TTAATGATAGTCTTCTGACG+AGG - chr2_4:60998731-60998750 None:intergenic 35.0%
AGAAGACTATCATTAAGACC+TGG + chr2_4:60998735-60998754 Msa0339730:CDS 35.0%
ATTCCTGTAATTCGAAGCTT+TGG - chr2_4:60998768-60998787 None:intergenic 35.0%
ACAACTTCATTGCACAGAAA+TGG + chr2_4:60998886-60998905 Msa0339730:CDS 35.0%
CAACTTCATTGCACAGAAAT+GGG + chr2_4:60998887-60998906 Msa0339730:CDS 35.0%
AATCCAAAAGTCTTCTTCCA+TGG + chr2_4:60999053-60999072 Msa0339730:CDS 35.0%
TATCAAACAACGATGAGAAG+TGG - chr2_4:60999139-60999158 None:intergenic 35.0%
AACGAAAACAATCGTGAAGA+AGG + chr2_4:60999358-60999377 Msa0339730:CDS 35.0%
GTTACTTGGTTTACTGAGTT+TGG - chr2_4:60997553-60997572 None:intergenic 35.0%
! ACCATTTTCACAATGGAACT+TGG + chr2_4:60998028-60998047 Msa0339730:CDS 35.0%
TCCAAGTTCCATTGTGAAAA+TGG - chr2_4:60998032-60998051 None:intergenic 35.0%
!!! CTTTTTTCTGGTTCTGTTGA+AGG - chr2_4:60998096-60998115 None:intergenic 35.0%
! AGTACCTCATCTAAAATCTC+TGG - chr2_4:60998213-60998232 None:intergenic 35.0%
CAACATTATGTGATGCTTGT+TGG - chr2_4:60998269-60998288 None:intergenic 35.0%
!! GCATCACATAATGTTGATTC+AGG + chr2_4:60998273-60998292 Msa0339730:CDS 35.0%
!! TCACATAATGTTGATTCAGG+AGG + chr2_4:60998276-60998295 Msa0339730:CDS 35.0%
!! TAATGTTGATTCAGGAGGAA+AGG + chr2_4:60998281-60998300 Msa0339730:CDS 35.0%
!! TTGTGCACATTGATTTAGCA+TGG - chr2_4:60998376-60998395 None:intergenic 35.0%
ATGAGCTACTCAAACAGATT+AGG + chr2_4:60998427-60998446 Msa0339730:CDS 35.0%
! GAATTTTCTATGGTTTCCAG+TGG + chr2_4:60998700-60998719 Msa0339730:CDS 35.0%
TTAATGATAGTCTTCTGACG+AGG - chr2_4:60998731-60998750 None:intergenic 35.0%
AGAAGACTATCATTAAGACC+TGG + chr2_4:60998735-60998754 Msa0339730:CDS 35.0%
ATTCCTGTAATTCGAAGCTT+TGG - chr2_4:60998768-60998787 None:intergenic 35.0%
ACAACTTCATTGCACAGAAA+TGG + chr2_4:60998886-60998905 Msa0339730:CDS 35.0%
CAACTTCATTGCACAGAAAT+GGG + chr2_4:60998887-60998906 Msa0339730:CDS 35.0%
AATCCAAAAGTCTTCTTCCA+TGG + chr2_4:60999053-60999072 Msa0339730:CDS 35.0%
TATCAAACAACGATGAGAAG+TGG - chr2_4:60999139-60999158 None:intergenic 35.0%
AACGAAAACAATCGTGAAGA+AGG + chr2_4:60999358-60999377 Msa0339730:CDS 35.0%
AAGCTCAAATGGACAATCCT+TGG + chr2_4:60997624-60997643 Msa0339730:CDS 40.0%
AGTCATCTTCTTCATCCATG+AGG - chr2_4:60997696-60997715 None:intergenic 40.0%
CATGGATGAAGAAGATGACT+TGG + chr2_4:60997696-60997715 Msa0339730:CDS 40.0%
! GCAGGAATGTTTAAGACTTC+AGG + chr2_4:60997738-60997757 Msa0339730:CDS 40.0%
! ATCGTAGAAGGACTTTTCAG+CGG - chr2_4:60997764-60997783 None:intergenic 40.0%
AAGTCCTTCTACGATGTTCT+TGG + chr2_4:60997769-60997788 Msa0339730:CDS 40.0%
! TCAGTGCTATCATTTGGTGA+AGG - chr2_4:60997805-60997824 None:intergenic 40.0%
AAGATTCGAATTCGACGACA+TGG - chr2_4:60997918-60997937 None:intergenic 40.0%
! GGTGCTAACCATTTTCACAA+TGG + chr2_4:60998021-60998040 Msa0339730:CDS 40.0%
TTCACAATGGAACTTGGAGT+TGG + chr2_4:60998034-60998053 Msa0339730:CDS 40.0%
TGTAATGAACAAGAGGGAAG+AGG + chr2_4:60998147-60998166 Msa0339730:CDS 40.0%
! ACCTCATCTAAAATCTCTGG+TGG - chr2_4:60998210-60998229 None:intergenic 40.0%
!! GAGGTACTGCTTTATCAGAA+AGG + chr2_4:60998225-60998244 Msa0339730:CDS 40.0%
AAATCAATGTGCACAAGCTG+TGG + chr2_4:60998380-60998399 Msa0339730:CDS 40.0%
CAAAGTAATGAGCTAAACGC+TGG - chr2_4:60998482-60998501 None:intergenic 40.0%
ACATCAAGAGGCTGATATGA+TGG - chr2_4:60998540-60998559 None:intergenic 40.0%
ATCATATCAGCCTCTTGATG+TGG + chr2_4:60998539-60998558 Msa0339730:CDS 40.0%
GTATTTGTCACTCTCTGCAA+AGG - chr2_4:60998615-60998634 None:intergenic 40.0%
AGACTATCATTAAGACCTGG+TGG + chr2_4:60998738-60998757 Msa0339730:CDS 40.0%
TTACAGGAATTGAGCTTCCT+CGG + chr2_4:60998778-60998797 Msa0339730:CDS 40.0%
AGGATTTAGACCTGCAGAAA+GGG + chr2_4:60998803-60998822 Msa0339730:CDS 40.0%
TGTCTATCTTTACATCCTCG+AGG - chr2_4:60998923-60998942 None:intergenic 40.0%
TCGAGGATGTAAAGATAGAC+AGG + chr2_4:60998922-60998941 Msa0339730:CDS 40.0%
TTCAATGCCACAGTTTCATC+AGG - chr2_4:60998993-60999012 None:intergenic 40.0%
AGCTTCAACACATCTTCTCT+TGG - chr2_4:60999020-60999039 None:intergenic 40.0%
AAGATGTGTTGAAGCTGATC+AGG + chr2_4:60999024-60999043 Msa0339730:CDS 40.0%
! ACTCCATGGAAGAAGACTTT+TGG - chr2_4:60999059-60999078 None:intergenic 40.0%
TTAAACCGAGTCGGGAAAAA+CGG - chr2_4:60999104-60999123 None:intergenic 40.0%
! GATTTTGTAGTCGATGTGGA+TGG + chr2_4:60999392-60999411 Msa0339730:CDS 40.0%
AAGCTCAAATGGACAATCCT+TGG + chr2_4:60997624-60997643 Msa0339730:CDS 40.0%
AGTCATCTTCTTCATCCATG+AGG - chr2_4:60997696-60997715 None:intergenic 40.0%
CATGGATGAAGAAGATGACT+TGG + chr2_4:60997696-60997715 Msa0339730:CDS 40.0%
! GCAGGAATGTTTAAGACTTC+AGG + chr2_4:60997738-60997757 Msa0339730:CDS 40.0%
! ATCGTAGAAGGACTTTTCAG+CGG - chr2_4:60997764-60997783 None:intergenic 40.0%
AAGTCCTTCTACGATGTTCT+TGG + chr2_4:60997769-60997788 Msa0339730:CDS 40.0%
! TCAGTGCTATCATTTGGTGA+AGG - chr2_4:60997805-60997824 None:intergenic 40.0%
AAGATTCGAATTCGACGACA+TGG - chr2_4:60997918-60997937 None:intergenic 40.0%
! GGTGCTAACCATTTTCACAA+TGG + chr2_4:60998021-60998040 Msa0339730:CDS 40.0%
TTCACAATGGAACTTGGAGT+TGG + chr2_4:60998034-60998053 Msa0339730:CDS 40.0%
TGTAATGAACAAGAGGGAAG+AGG + chr2_4:60998147-60998166 Msa0339730:CDS 40.0%
! ACCTCATCTAAAATCTCTGG+TGG - chr2_4:60998210-60998229 None:intergenic 40.0%
!! GAGGTACTGCTTTATCAGAA+AGG + chr2_4:60998225-60998244 Msa0339730:CDS 40.0%
AAATCAATGTGCACAAGCTG+TGG + chr2_4:60998380-60998399 Msa0339730:CDS 40.0%
CAAAGTAATGAGCTAAACGC+TGG - chr2_4:60998482-60998501 None:intergenic 40.0%
ACATCAAGAGGCTGATATGA+TGG - chr2_4:60998540-60998559 None:intergenic 40.0%
ATCATATCAGCCTCTTGATG+TGG + chr2_4:60998539-60998558 Msa0339730:CDS 40.0%
GTATTTGTCACTCTCTGCAA+AGG - chr2_4:60998615-60998634 None:intergenic 40.0%
AGACTATCATTAAGACCTGG+TGG + chr2_4:60998738-60998757 Msa0339730:CDS 40.0%
TTACAGGAATTGAGCTTCCT+CGG + chr2_4:60998778-60998797 Msa0339730:CDS 40.0%
AGGATTTAGACCTGCAGAAA+GGG + chr2_4:60998803-60998822 Msa0339730:CDS 40.0%
TGTCTATCTTTACATCCTCG+AGG - chr2_4:60998923-60998942 None:intergenic 40.0%
TCGAGGATGTAAAGATAGAC+AGG + chr2_4:60998922-60998941 Msa0339730:CDS 40.0%
TTCAATGCCACAGTTTCATC+AGG - chr2_4:60998993-60999012 None:intergenic 40.0%
AGCTTCAACACATCTTCTCT+TGG - chr2_4:60999020-60999039 None:intergenic 40.0%
AAGATGTGTTGAAGCTGATC+AGG + chr2_4:60999024-60999043 Msa0339730:CDS 40.0%
! ACTCCATGGAAGAAGACTTT+TGG - chr2_4:60999059-60999078 None:intergenic 40.0%
TTAAACCGAGTCGGGAAAAA+CGG - chr2_4:60999104-60999123 None:intergenic 40.0%
! GATTTTGTAGTCGATGTGGA+TGG + chr2_4:60999392-60999411 Msa0339730:CDS 40.0%
CCAGAAGAAGAAGGTGAAGA+TGG - chr2_4:60997601-60997620 None:intergenic 45.0%
CCATCTTCACCTTCTTCTTC+TGG + chr2_4:60997598-60997617 Msa0339730:CDS 45.0%
TCTTCTGGCACAAGCTCAAA+TGG + chr2_4:60997613-60997632 Msa0339730:CDS 45.0%
!! GGAATATCTTGTCGGTTCCA+AGG - chr2_4:60997644-60997663 None:intergenic 45.0%
AAACATTCCTGCAGCATGCA+AGG - chr2_4:60997730-60997749 None:intergenic 45.0%
ATGGCCAAGAACATCGTAGA+AGG - chr2_4:60997776-60997795 None:intergenic 45.0%
! CATTTGGTGAAGGCTGGTTA+TGG - chr2_4:60997795-60997814 None:intergenic 45.0%
! TGCTATCATTTGGTGAAGGC+TGG - chr2_4:60997801-60997820 None:intergenic 45.0%
TCTGGGTCAGTGCTATCATT+TGG - chr2_4:60997811-60997830 None:intergenic 45.0%
!! AATGATAGCACTGACCCAGA+TGG + chr2_4:60997811-60997830 Msa0339730:CDS 45.0%
CGCGATCGAAATTTCCATCT+GGG - chr2_4:60997828-60997847 None:intergenic 45.0%
!!! GCTGCTAGTTTTGAGAGAAG+TGG + chr2_4:60997847-60997866 Msa0339730:CDS 45.0%
GAATGACATTCAGCCAGTGA+CGG + chr2_4:60998056-60998075 Msa0339730:CDS 45.0%
AAAACTTCTTCCACCGTCAC+TGG - chr2_4:60998072-60998091 None:intergenic 45.0%
! GCCACCAGAGATTTTAGATG+AGG + chr2_4:60998206-60998225 Msa0339730:CDS 45.0%
ATCTACGACAAACAATGCCG+CGG + chr2_4:60998335-60998354 Msa0339730:CDS 45.0%
! CCTGTAATTCGAAGCTTTGG+AGG - chr2_4:60998765-60998784 None:intergenic 45.0%
CCTCCAAAGCTTCGAATTAC+AGG + chr2_4:60998762-60998781 Msa0339730:CDS 45.0%
CTTTCTGCAGGTCTAAATCC+TGG - chr2_4:60998804-60998823 None:intergenic 45.0%
CAGGATTTAGACCTGCAGAA+AGG + chr2_4:60998802-60998821 Msa0339730:CDS 45.0%
ATTTAGACCTGCAGAAAGGG+TGG + chr2_4:60998806-60998825 Msa0339730:CDS 45.0%
GAACCTTCCTGATGAAACTG+TGG + chr2_4:60998983-60999002 Msa0339730:CDS 45.0%
ATGCCACAGTTTCATCAGGA+AGG - chr2_4:60998989-60999008 None:intergenic 45.0%
TTCTTCCATGGAGTTGCCAA+TGG + chr2_4:60999065-60999084 Msa0339730:CDS 45.0%
AACTTCCATTGGCAACTCCA+TGG - chr2_4:60999073-60999092 None:intergenic 45.0%
!!! GTTTTTCCCGACTCGGTTTA+AGG + chr2_4:60999103-60999122 Msa0339730:CDS 45.0%
AGTGCTTCCTTAAACCGAGT+CGG - chr2_4:60999113-60999132 None:intergenic 45.0%
AGAGATCGATGCTTGAGAGA+GGG + chr2_4:60999192-60999211 Msa0339730:CDS 45.0%
ATGCTTGAGAGAGGGTTGTT+TGG + chr2_4:60999200-60999219 Msa0339730:CDS 45.0%
AGGTCAGGAATAAGAGAGCT+GGG + chr2_4:60999297-60999316 Msa0339730:CDS 45.0%
TGGGTTCAAGCAAATCAGGT+TGG + chr2_4:60999316-60999335 Msa0339730:CDS 45.0%
TAGTCGATGTGGATGGTAAG+TGG + chr2_4:60999399-60999418 Msa0339730:CDS 45.0%
AGTCGATGTGGATGGTAAGT+GGG + chr2_4:60999400-60999419 Msa0339730:CDS 45.0%
!! GATGGTAAGTGGGTTTTGCA+GGG + chr2_4:60999410-60999429 Msa0339730:CDS 45.0%
CCAGAAGAAGAAGGTGAAGA+TGG - chr2_4:60997601-60997620 None:intergenic 45.0%
CCATCTTCACCTTCTTCTTC+TGG + chr2_4:60997598-60997617 Msa0339730:CDS 45.0%
TCTTCTGGCACAAGCTCAAA+TGG + chr2_4:60997613-60997632 Msa0339730:CDS 45.0%
!! GGAATATCTTGTCGGTTCCA+AGG - chr2_4:60997644-60997663 None:intergenic 45.0%
AAACATTCCTGCAGCATGCA+AGG - chr2_4:60997730-60997749 None:intergenic 45.0%
ATGGCCAAGAACATCGTAGA+AGG - chr2_4:60997776-60997795 None:intergenic 45.0%
! CATTTGGTGAAGGCTGGTTA+TGG - chr2_4:60997795-60997814 None:intergenic 45.0%
! TGCTATCATTTGGTGAAGGC+TGG - chr2_4:60997801-60997820 None:intergenic 45.0%
TCTGGGTCAGTGCTATCATT+TGG - chr2_4:60997811-60997830 None:intergenic 45.0%
!! AATGATAGCACTGACCCAGA+TGG + chr2_4:60997811-60997830 Msa0339730:CDS 45.0%
CGCGATCGAAATTTCCATCT+GGG - chr2_4:60997828-60997847 None:intergenic 45.0%
!!! GCTGCTAGTTTTGAGAGAAG+TGG + chr2_4:60997847-60997866 Msa0339730:CDS 45.0%
GAATGACATTCAGCCAGTGA+CGG + chr2_4:60998056-60998075 Msa0339730:CDS 45.0%
AAAACTTCTTCCACCGTCAC+TGG - chr2_4:60998072-60998091 None:intergenic 45.0%
! GCCACCAGAGATTTTAGATG+AGG + chr2_4:60998206-60998225 Msa0339730:CDS 45.0%
ATCTACGACAAACAATGCCG+CGG + chr2_4:60998335-60998354 Msa0339730:CDS 45.0%
! CCTGTAATTCGAAGCTTTGG+AGG - chr2_4:60998765-60998784 None:intergenic 45.0%
CCTCCAAAGCTTCGAATTAC+AGG + chr2_4:60998762-60998781 Msa0339730:CDS 45.0%
CTTTCTGCAGGTCTAAATCC+TGG - chr2_4:60998804-60998823 None:intergenic 45.0%
CAGGATTTAGACCTGCAGAA+AGG + chr2_4:60998802-60998821 Msa0339730:CDS 45.0%
ATTTAGACCTGCAGAAAGGG+TGG + chr2_4:60998806-60998825 Msa0339730:CDS 45.0%
GAACCTTCCTGATGAAACTG+TGG + chr2_4:60998983-60999002 Msa0339730:CDS 45.0%
ATGCCACAGTTTCATCAGGA+AGG - chr2_4:60998989-60999008 None:intergenic 45.0%
TTCTTCCATGGAGTTGCCAA+TGG + chr2_4:60999065-60999084 Msa0339730:CDS 45.0%
AACTTCCATTGGCAACTCCA+TGG - chr2_4:60999073-60999092 None:intergenic 45.0%
!!! GTTTTTCCCGACTCGGTTTA+AGG + chr2_4:60999103-60999122 Msa0339730:CDS 45.0%
AGTGCTTCCTTAAACCGAGT+CGG - chr2_4:60999113-60999132 None:intergenic 45.0%
AGAGATCGATGCTTGAGAGA+GGG + chr2_4:60999192-60999211 Msa0339730:CDS 45.0%
ATGCTTGAGAGAGGGTTGTT+TGG + chr2_4:60999200-60999219 Msa0339730:CDS 45.0%
AGGTCAGGAATAAGAGAGCT+GGG + chr2_4:60999297-60999316 Msa0339730:CDS 45.0%
TGGGTTCAAGCAAATCAGGT+TGG + chr2_4:60999316-60999335 Msa0339730:CDS 45.0%
TAGTCGATGTGGATGGTAAG+TGG + chr2_4:60999399-60999418 Msa0339730:CDS 45.0%
AGTCGATGTGGATGGTAAGT+GGG + chr2_4:60999400-60999419 Msa0339730:CDS 45.0%
!! GATGGTAAGTGGGTTTTGCA+GGG + chr2_4:60999410-60999429 Msa0339730:CDS 45.0%
GAGCTTGTGCCAGAAGAAGA+AGG - chr2_4:60997610-60997629 None:intergenic 50.0%
GCAAAAACCTTGCATGCTGC+AGG + chr2_4:60997720-60997739 Msa0339730:CDS 50.0%
GCGCGATCGAAATTTCCATC+TGG - chr2_4:60997829-60997848 None:intergenic 50.0%
CCTTGTGTCTCGATGACAAC+TGG - chr2_4:60998003-60998022 None:intergenic 50.0%
CCAGTTGTCATCGAGACACA+AGG + chr2_4:60998000-60998019 Msa0339730:CDS 50.0%
! AGGCAACACTCTTCGCCTTT+TGG + chr2_4:60998447-60998466 Msa0339730:CDS 50.0%
! CACTCTTCGCCTTTTGGTGA+TGG + chr2_4:60998453-60998472 Msa0339730:CDS 50.0%
GATAGTCTTCTGACGAGGCA+CGG - chr2_4:60998726-60998745 None:intergenic 50.0%
CTTTACATCCTCGAGGCAGA+TGG - chr2_4:60998916-60998935 None:intergenic 50.0%
CGGTGCGCTATAACTTCCAT+TGG - chr2_4:60999084-60999103 None:intergenic 50.0%
GTGCTTCCTTAAACCGAGTC+GGG - chr2_4:60999112-60999131 None:intergenic 50.0%
GATCTCTGCATGTCTTCACG+AGG - chr2_4:60999179-60999198 None:intergenic 50.0%
CAGAGATCGATGCTTGAGAG+AGG + chr2_4:60999191-60999210 Msa0339730:CDS 50.0%
! TAGCTTGTGAGGGTGCAGAA+AGG + chr2_4:60999243-60999262 Msa0339730:CDS 50.0%
! AGCTTGTGAGGGTGCAGAAA+GGG + chr2_4:60999244-60999263 Msa0339730:CDS 50.0%
AGGGTGCAGAAAGGGTTGAA+AGG + chr2_4:60999252-60999271 Msa0339730:CDS 50.0%
CAGGTCAGGAATAAGAGAGC+TGG + chr2_4:60999296-60999315 Msa0339730:CDS 50.0%
GAGCTGGGTTCAAGCAAATC+AGG + chr2_4:60999312-60999331 Msa0339730:CDS 50.0%
!! GGATGGTAAGTGGGTTTTGC+AGG + chr2_4:60999409-60999428 Msa0339730:CDS 50.0%
!! GTAAGTGGGTTTTGCAGGGA+TGG + chr2_4:60999414-60999433 Msa0339730:CDS 50.0%
! TGGGTTTTGCAGGGATGGAA+GGG + chr2_4:60999419-60999438 Msa0339730:CDS 50.0%
GAGCTTGTGCCAGAAGAAGA+AGG - chr2_4:60997610-60997629 None:intergenic 50.0%
GCAAAAACCTTGCATGCTGC+AGG + chr2_4:60997720-60997739 Msa0339730:CDS 50.0%
GCGCGATCGAAATTTCCATC+TGG - chr2_4:60997829-60997848 None:intergenic 50.0%
CCTTGTGTCTCGATGACAAC+TGG - chr2_4:60998003-60998022 None:intergenic 50.0%
CCAGTTGTCATCGAGACACA+AGG + chr2_4:60998000-60998019 Msa0339730:CDS 50.0%
! AGGCAACACTCTTCGCCTTT+TGG + chr2_4:60998447-60998466 Msa0339730:CDS 50.0%
! CACTCTTCGCCTTTTGGTGA+TGG + chr2_4:60998453-60998472 Msa0339730:CDS 50.0%
GATAGTCTTCTGACGAGGCA+CGG - chr2_4:60998726-60998745 None:intergenic 50.0%
CTTTACATCCTCGAGGCAGA+TGG - chr2_4:60998916-60998935 None:intergenic 50.0%
CGGTGCGCTATAACTTCCAT+TGG - chr2_4:60999084-60999103 None:intergenic 50.0%
GTGCTTCCTTAAACCGAGTC+GGG - chr2_4:60999112-60999131 None:intergenic 50.0%
GATCTCTGCATGTCTTCACG+AGG - chr2_4:60999179-60999198 None:intergenic 50.0%
CAGAGATCGATGCTTGAGAG+AGG + chr2_4:60999191-60999210 Msa0339730:CDS 50.0%
! TAGCTTGTGAGGGTGCAGAA+AGG + chr2_4:60999243-60999262 Msa0339730:CDS 50.0%
! AGCTTGTGAGGGTGCAGAAA+GGG + chr2_4:60999244-60999263 Msa0339730:CDS 50.0%
AGGGTGCAGAAAGGGTTGAA+AGG + chr2_4:60999252-60999271 Msa0339730:CDS 50.0%
CAGGTCAGGAATAAGAGAGC+TGG + chr2_4:60999296-60999315 Msa0339730:CDS 50.0%
GAGCTGGGTTCAAGCAAATC+AGG + chr2_4:60999312-60999331 Msa0339730:CDS 50.0%
!! GGATGGTAAGTGGGTTTTGC+AGG + chr2_4:60999409-60999428 Msa0339730:CDS 50.0%
!! GTAAGTGGGTTTTGCAGGGA+TGG + chr2_4:60999414-60999433 Msa0339730:CDS 50.0%
! TGGGTTTTGCAGGGATGGAA+GGG + chr2_4:60999419-60999438 Msa0339730:CDS 50.0%
TGACATTCAGCCAGTGACGG+TGG + chr2_4:60998059-60998078 Msa0339730:CDS 55.0%
CTGTGGCGAGCTACGATCAA+AGG + chr2_4:60998397-60998416 Msa0339730:CDS 55.0%
CGCTGGAGTCCATCACCAAA+AGG - chr2_4:60998465-60998484 None:intergenic 55.0%
CCAGCAGTTGCCACATCAAG+AGG - chr2_4:60998552-60998571 None:intergenic 55.0%
CCTCTTGATGTGGCAACTGC+TGG + chr2_4:60998549-60998568 Msa0339730:CDS 55.0%
CACTCTCTGCAAAGGAGACG+CGG - chr2_4:60998607-60998626 None:intergenic 55.0%
TAGACCTGCAGAAAGGGTGG+AGG + chr2_4:60998809-60998828 Msa0339730:CDS 55.0%
GTTTCCTCCACCCTTTCTGC+AGG - chr2_4:60998816-60998835 None:intergenic 55.0%
GCAGAAAGGGTGGAGGAAAC+CGG + chr2_4:60998816-60998835 Msa0339730:CDS 55.0%
AAAGGGTGGAGGAAACCGGA+CGG + chr2_4:60998820-60998839 Msa0339730:CDS 55.0%
!! GTAGTTTGCTAGACGCCGTC+CGG - chr2_4:60998838-60998857 None:intergenic 55.0%
ATGGGAAACCATCTGCCTCG+AGG + chr2_4:60998905-60998924 Msa0339730:CDS 55.0%
TGCCACTGCTTGTAGGTCTC+AGG - chr2_4:60999278-60999297 None:intergenic 55.0%
TGAGACCTACAAGCAGTGGC+AGG + chr2_4:60999277-60999296 Msa0339730:CDS 55.0%
! GTGGGTTTTGCAGGGATGGA+AGG + chr2_4:60999418-60999437 Msa0339730:CDS 55.0%
TGACATTCAGCCAGTGACGG+TGG + chr2_4:60998059-60998078 Msa0339730:CDS 55.0%
CTGTGGCGAGCTACGATCAA+AGG + chr2_4:60998397-60998416 Msa0339730:CDS 55.0%
CGCTGGAGTCCATCACCAAA+AGG - chr2_4:60998465-60998484 None:intergenic 55.0%
CCAGCAGTTGCCACATCAAG+AGG - chr2_4:60998552-60998571 None:intergenic 55.0%
CCTCTTGATGTGGCAACTGC+TGG + chr2_4:60998549-60998568 Msa0339730:CDS 55.0%
CACTCTCTGCAAAGGAGACG+CGG - chr2_4:60998607-60998626 None:intergenic 55.0%
TAGACCTGCAGAAAGGGTGG+AGG + chr2_4:60998809-60998828 Msa0339730:CDS 55.0%
GTTTCCTCCACCCTTTCTGC+AGG - chr2_4:60998816-60998835 None:intergenic 55.0%
GCAGAAAGGGTGGAGGAAAC+CGG + chr2_4:60998816-60998835 Msa0339730:CDS 55.0%
AAAGGGTGGAGGAAACCGGA+CGG + chr2_4:60998820-60998839 Msa0339730:CDS 55.0%
!! GTAGTTTGCTAGACGCCGTC+CGG - chr2_4:60998838-60998857 None:intergenic 55.0%
ATGGGAAACCATCTGCCTCG+AGG + chr2_4:60998905-60998924 Msa0339730:CDS 55.0%
TGCCACTGCTTGTAGGTCTC+AGG - chr2_4:60999278-60999297 None:intergenic 55.0%
TGAGACCTACAAGCAGTGGC+AGG + chr2_4:60999277-60999296 Msa0339730:CDS 55.0%
! GTGGGTTTTGCAGGGATGGA+AGG + chr2_4:60999418-60999437 Msa0339730:CDS 55.0%
GACAAACAATGCCGCGGCAG+TGG + chr2_4:60998341-60998360 Msa0339730:CDS 60.0%
! ATGCCGCGGCAGTGGATTTG+TGG + chr2_4:60998349-60998368 Msa0339730:CDS 60.0%
GGTCCACAAATCCACTGCCG+CGG - chr2_4:60998355-60998374 None:intergenic 60.0%
!! CGAAGCTTTGGAGGACCACC+AGG - chr2_4:60998756-60998775 None:intergenic 60.0%
GGAATTGAGCTTCCTCGGCC+AGG + chr2_4:60998783-60998802 Msa0339730:CDS 60.0%
GCAGGTCTAAATCCTGGCCG+AGG - chr2_4:60998798-60998817 None:intergenic 60.0%
!! GCGCACCGTTTTTCCCGACT+CGG + chr2_4:60999096-60999115 Msa0339730:CDS 60.0%
GGCCTGAGACCTACAAGCAG+TGG + chr2_4:60999273-60999292 Msa0339730:CDS 60.0%
CCTGACCTGCCACTGCTTGT+AGG - chr2_4:60999285-60999304 None:intergenic 60.0%
CCTACAAGCAGTGGCAGGTC+AGG + chr2_4:60999282-60999301 Msa0339730:CDS 60.0%
GACAAACAATGCCGCGGCAG+TGG + chr2_4:60998341-60998360 Msa0339730:CDS 60.0%
! ATGCCGCGGCAGTGGATTTG+TGG + chr2_4:60998349-60998368 Msa0339730:CDS 60.0%
GGTCCACAAATCCACTGCCG+CGG - chr2_4:60998355-60998374 None:intergenic 60.0%
!! CGAAGCTTTGGAGGACCACC+AGG - chr2_4:60998756-60998775 None:intergenic 60.0%
GGAATTGAGCTTCCTCGGCC+AGG + chr2_4:60998783-60998802 Msa0339730:CDS 60.0%
GCAGGTCTAAATCCTGGCCG+AGG - chr2_4:60998798-60998817 None:intergenic 60.0%
!! GCGCACCGTTTTTCCCGACT+CGG + chr2_4:60999096-60999115 Msa0339730:CDS 60.0%
GGCCTGAGACCTACAAGCAG+TGG + chr2_4:60999273-60999292 Msa0339730:CDS 60.0%
CCTGACCTGCCACTGCTTGT+AGG - chr2_4:60999285-60999304 None:intergenic 60.0%
CCTACAAGCAGTGGCAGGTC+AGG + chr2_4:60999282-60999301 Msa0339730:CDS 60.0%
TTCTGACGAGGCACGGCCAC+TGG - chr2_4:60998719-60998738 None:intergenic 65.0%
TTCTGACGAGGCACGGCCAC+TGG - chr2_4:60998719-60998738 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 60997511 60999481 60997511 ID=Msa0339730;Name=Msa0339730
chr2_4 mRNA 60997511 60999481 60997511 ID=Msa0339730-mRNA-1;Parent=Msa0339730;Name=Msa0339730-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|656
chr2_4 exon 60997511 60999481 60997511 ID=Msa0339730-mRNA-1:exon:19058;Parent=Msa0339730-mRNA-1
chr2_4 CDS 60997511 60999481 60997511 ID=Msa0339730-mRNA-1:cds;Parent=Msa0339730-mRNA-1
Gene Sequence

>Msa0339730

ATGCTTTCTCCAAACACTTTTCTGCAAGATTTTAGTGTTCCAAACTCAGTAAACCAAGTAACAAATGGGTTTGAATTAGAAGATTTACCATCTTCACCTTCTTCTTCTGGCACAAGCTCAAATGGACAATCCTTGGAACCGACAAGATATTCCAATAACATCCTAAGATACATAAATGACATCCTCATGGATGAAGAAGATGACTTGGAGCAAAAACCTTGCATGCTGCAGGAATGTTTAAGACTTCAGGCCGCTGAAAAGTCCTTCTACGATGTTCTTGGCCATAACCAGCCTTCACCAAATGATAGCACTGACCCAGATGGAAATTTCGATCGCGCTGCTAGTTTTGAGAGAAGTGGCAGCAGCTGCATCGCTGATAACTCATATGAATCTAACTTGGTCAACCATGTCGTCGAATTCGAATCTTCTTCCATACAACTACAATGTCCTTTAATAGAAAAAAACTACTATGACTTGACAGAAGCTGATCCAGTTGTCATCGAGACACAAGGTGCTAACCATTTTCACAATGGAACTTGGAGTTGGAATGACATTCAGCCAGTGACGGTGGAAGAAGTTTTACCTTCAACAGAACCAGAAAAAAGAAGTCGTAAGATGGATAGTGATAATGATACTTGTAATGAACAAGAGGGAAGAGGTAGCAAAGTTTCAGCTATTTTCTCTGATGAATTAGAGCCACCAGAGATTTTAGATGAGGTACTGCTTTATCAGAAAGGAAAGACTCTCACTCAACACCAACAAGCATCACATAATGTTGATTCAGGAGGAAAGGCAAAAGAAACACATTCGCGTTTGAAGAAAGCATCTACGACAAACAATGCCGCGGCAGTGGATTTGTGGACCATGCTAAATCAATGTGCACAAGCTGTGGCGAGCTACGATCAAAGGAATACAGATGAGCTACTCAAACAGATTAGGCAACACTCTTCGCCTTTTGGTGATGGACTCCAGCGTTTAGCTCATTACTTTGCTAATGGTCTTGAGATAAGATTGGCTGCTGAGACACCATCATATCAGCCTCTTGATGTGGCAACTGCTGGAGATATGTTGAAAGCTTACAAACTATTTGTTACCGCGTCTCCTTTGCAGAGAGTGACAAATACTTTGCTAACCAAAACGATTTTCAAAATAGTGAAGAACGAGAGCAGCGTTCATGTTATTGATTTTGGAATTTTCTATGGTTTCCAGTGGCCGTGCCTCGTCAGAAGACTATCATTAAGACCTGGTGGTCCTCCAAAGCTTCGAATTACAGGAATTGAGCTTCCTCGGCCAGGATTTAGACCTGCAGAAAGGGTGGAGGAAACCGGACGGCGTCTAGCAAACTACTGCAAGAAGTTCAATGTACCATTTGAATACAACTTCATTGCACAGAAATGGGAAACCATCTGCCTCGAGGATGTAAAGATAGACAGGAATGAAATAACTCTTGTTAGCTGTTTGTACAGACTGAAGAACCTTCCTGATGAAACTGTGGCATTGAATTGTCCAAGAGAAGATGTGTTGAAGCTGATCAGGAAGATTAATCCAAAAGTCTTCTTCCATGGAGTTGCCAATGGAAGTTATAGCGCACCGTTTTTCCCGACTCGGTTTAAGGAAGCACTCTACCACTTCTCATCGTTGTTTGATATGTTTGAGGCTAACGTGCCTCGTGAAGACATGCAGAGATCGATGCTTGAGAGAGGGTTGTTTGGAAGAGATGCCATAAATGTTATAGCTTGTGAGGGTGCAGAAAGGGTTGAAAGGCCTGAGACCTACAAGCAGTGGCAGGTCAGGAATAAGAGAGCTGGGTTCAAGCAAATCAGGTTGGATTCTGATTTAGTGAATGAAACGAAAACAATCGTGAAGAAGGAATACCATAAAGATTTTGTAGTCGATGTGGATGGTAAGTGGGTTTTGCAGGGATGGAAGGGACGCATTTTGAATGCTTTATCAGCTTGGGTTCCTGCTTAA
Protein sequence

>Msa0339730

MLSPNTFLQDFSVPNSVNQVTNGFELEDLPSSPSSSGTSSNGQSLEPTRYSNNILRYINDILMDEEDDLEQKPCMLQECLRLQAAEKSFYDVLGHNQPSPNDSTDPDGNFDRAASFERSGSSCIADNSYESNLVNHVVEFESSSIQLQCPLIEKNYYDLTEADPVVIETQGANHFHNGTWSWNDIQPVTVEEVLPSTEPEKRSRKMDSDNDTCNEQEGRGSKVSAIFSDELEPPEILDEVLLYQKGKTLTQHQQASHNVDSGGKAKETHSRLKKASTTNNAAAVDLWTMLNQCAQAVASYDQRNTDELLKQIRQHSSPFGDGLQRLAHYFANGLEIRLAAETPSYQPLDVATAGDMLKAYKLFVTASPLQRVTNTLLTKTIFKIVKNESSVHVIDFGIFYGFQWPCLVRRLSLRPGGPPKLRITGIELPRPGFRPAERVEETGRRLANYCKKFNVPFEYNFIAQKWETICLEDVKIDRNEITLVSCLYRLKNLPDETVALNCPREDVLKLIRKINPKVFFHGVANGSYSAPFFPTRFKEALYHFSSLFDMFEANVPREDMQRSMLERGLFGRDAINVIACEGAERVERPETYKQWQVRNKRAGFKQIRLDSDLVNETKTIVKKEYHKDFVVDVDGKWVLQGWKGRILNALSAWVPA*