AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0584580


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0584580 MtrunA17_Chr4g0021961 96.364 110 4 0 1 110 1 110 3.41e-72 210
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGCAACCCTCTTTATCTT+AGG 0.308528 4_2:+4317028 None:intergenic
GATAAAGAGGGTTGCTGTTA+AGG 0.330238 4_2:-4317023 Msa0584580:CDS
AGACAAGGATATGAGCTTCT+TGG 0.379115 4_2:-4317178 Msa0584580:CDS
CTAAAAGACCTAAGATAAAG+AGG 0.402553 4_2:-4317036 Msa0584580:CDS
CTAGCTCCCCTCTTCCTACT+AGG 0.437842 4_2:-4316919 Msa0584580:CDS
TATGAGCTTCTTGGTTTACA+AGG 0.464864 4_2:-4317169 Msa0584580:CDS
CCTACTAGGAAGATCCTTTG+AGG 0.470260 4_2:-4316905 None:intergenic
CCTCAAAGGATCTTCCTAGT+AGG 0.477106 4_2:+4316905 None:intergenic
GTTGCGAACGAGTGTGTTCA+TGG 0.491971 4_2:+4317222 None:intergenic
ATATCCTTGTCTCCTGCTGA+AGG 0.525798 4_2:+4317189 None:intergenic
GGATCTTCCTAGTAGGAAGA+GGG 0.550819 4_2:+4316912 None:intergenic
ACACCACTCTCACCTTCAGC+AGG 0.551528 4_2:-4317201 Msa0584580:CDS
AGGATCTTCCTAGTAGGAAG+AGG 0.558203 4_2:+4316911 None:intergenic
TCTCCTGCTGAAGGTGAGAG+TGG 0.568581 4_2:+4317198 None:intergenic
CTCACCTTCAGCAGGAGACA+AGG 0.589904 4_2:-4317193 Msa0584580:CDS
ATGAGCTTCTTGGTTTACAA+GGG 0.601511 4_2:-4317168 Msa0584580:CDS
TAAAAGACCTAAGATAAAGA+GGG 0.605297 4_2:-4317035 Msa0584580:CDS
GACGCAGAAGAGCCAAACAA+AGG 0.632309 4_2:-4317114 Msa0584580:CDS
GATCTTCCTAGTAGGAAGAG+GGG 0.633617 4_2:+4316913 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAAAGACCTAAGATAAAGA+GGG - chr4_2:4317092-4317111 Msa0584580:CDS 25.0%
! GAGAAAGATTTTCCTTTGTT+TGG + chr4_2:4317028-4317047 None:intergenic 30.0%
! CTCTTTATCTTAGGTCTTTT+AGG + chr4_2:4317093-4317112 None:intergenic 30.0%
CTAAAAGACCTAAGATAAAG+AGG - chr4_2:4317091-4317110 Msa0584580:CDS 30.0%
!!! GTTTTGTTGTTTATGAGAAC+TGG - chr4_2:4317147-4317166 Msa0584580:CDS 30.0%
TATGAGCTTCTTGGTTTACA+AGG - chr4_2:4316958-4316977 Msa0584580:CDS 35.0%
ATGAGCTTCTTGGTTTACAA+GGG - chr4_2:4316959-4316978 Msa0584580:CDS 35.0%
AGACAAGGATATGAGCTTCT+TGG - chr4_2:4316949-4316968 Msa0584580:CDS 40.0%
ACAGCAACCCTCTTTATCTT+AGG + chr4_2:4317102-4317121 None:intergenic 40.0%
! GATAAAGAGGGTTGCTGTTA+AGG - chr4_2:4317104-4317123 Msa0584580:CDS 40.0%
ATATCCTTGTCTCCTGCTGA+AGG + chr4_2:4316941-4316960 None:intergenic 45.0%
GATCTTCCTAGTAGGAAGAG+GGG + chr4_2:4317217-4317236 None:intergenic 45.0%
GGATCTTCCTAGTAGGAAGA+GGG + chr4_2:4317218-4317237 None:intergenic 45.0%
AGGATCTTCCTAGTAGGAAG+AGG + chr4_2:4317219-4317238 None:intergenic 45.0%
GACGCAGAAGAGCCAAACAA+AGG - chr4_2:4317013-4317032 Msa0584580:CDS 50.0%
ACACCACTCTCACCTTCAGC+AGG - chr4_2:4316926-4316945 Msa0584580:CDS 55.0%
TCTCCTGCTGAAGGTGAGAG+TGG + chr4_2:4316932-4316951 None:intergenic 55.0%
CTCACCTTCAGCAGGAGACA+AGG - chr4_2:4316934-4316953 Msa0584580:CDS 55.0%
CTAGCTCCCCTCTTCCTACT+AGG - chr4_2:4317208-4317227 Msa0584580:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 4316907 4317242 4316907 ID=Msa0584580;Name=Msa0584580
chr4_2 mRNA 4316907 4317242 4316907 ID=Msa0584580-mRNA-1;Parent=Msa0584580;Name=Msa0584580-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|111
chr4_2 exon 4316907 4317242 4316907 ID=Msa0584580-mRNA-1:exon:1132;Parent=Msa0584580-mRNA-1
chr4_2 CDS 4316907 4317242 4316907 ID=Msa0584580-mRNA-1:cds;Parent=Msa0584580-mRNA-1
Gene Sequence

>Msa0584580

ATGAACACACTCGTTCGCAACACCACTCTCACCTTCAGCAGGAGACAAGGATATGAGCTTCTTGGTTTACAAGGGAAAGAGAAAACACTCAAATCAGTCAGCTACAGACGCAGAAGAGCCAAACAAAGGAAAATCTTTCTCACAACATATAAACTATCTTCTTTGAATACTTTTGTAGAACAGCCTAAAAGACCTAAGATAAAGAGGGTTGCTGTTAAGGTTAAGAAAATTGTTAACTCTGTTTTGTTGTTTATGAGAACTGGTTCTTTTAGATCATGTAGTTCTAAGTCTGCTATTTCTGCTAGCTCCCCTCTTCCTACTAGGAAGATCCTTTGA

Protein sequence

>Msa0584580

MNTLVRNTTLTFSRRQGYELLGLQGKEKTLKSVSYRRRRAKQRKIFLTTYKLSSLNTFVEQPKRPKIKRVAVKVKKIVNSVLLFMRTGSFRSCSSKSAISASSPLPTRKIL*