AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0223130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0223130 MtrunA17_Chr2g0328431 96.341 656 24 0 1 656 1 656 0.0 1318
Msa0223130 MtrunA17_Chr2g0328411 92.049 654 52 0 1 654 1 654 0.0 1214
Msa0223130 MtrunA17_Chr2g0328401 79.560 636 129 1 1 635 1 636 0.0 1043
Msa0223130 MtrunA17_Chr4g0031741 53.613 692 245 13 1 656 1 652 0.0 674
Msa0223130 MtrunA17_Chr7g0238891 44.088 685 303 14 44 656 66 742 0.0 534
Msa0223130 MtrunA17_Chr2g0328391 43.028 667 345 14 4 656 26 671 7.92e-172 506
Msa0223130 MtrunA17_Chr4g0031701 45.556 630 311 12 38 656 20 628 8.32e-171 501
Msa0223130 MtrunA17_Chr4g0031711 43.388 673 311 18 38 656 79 735 1.58e-168 499
Msa0223130 MtrunA17_Chr4g0031721 52.878 469 202 6 200 656 225 686 1.67e-167 495
Msa0223130 MtrunA17_Chr4g0031721 43.662 71 38 1 29 99 13 81 9.39e-11 65.1
Msa0223130 MtrunA17_Chr4g0031731 45.238 630 313 12 38 656 20 628 2.40e-167 493
Msa0223130 MtrunA17_Chr2g0328381 46.189 433 220 6 229 656 219 643 1.44e-131 401
Msa0223130 MtrunA17_Chr2g0328341 46.651 433 217 7 229 656 217 640 5.93e-131 399
Msa0223130 MtrunA17_Chr2g0328361 46.825 378 169 6 283 656 268 617 2.85e-114 355
Msa0223130 MtrunA17_Chr2g0328371 51.562 256 119 2 356 610 1 252 3.95e-92 285
Msa0223130 MtrunA17_Chr5g0447691 32.888 374 243 7 285 653 173 543 1.02e-56 201
Msa0223130 MtrunA17_Chr2g0290241 30.133 375 255 6 284 653 52 424 2.07e-56 197
Msa0223130 MtrunA17_Chr2g0319091 31.714 391 261 5 267 653 190 578 1.82e-55 198
Msa0223130 MtrunA17_Chr4g0076091 28.780 410 274 8 259 654 138 543 2.80e-52 189
Msa0223130 MtrunA17_Chr7g0224271 29.337 392 245 5 282 653 293 672 6.43e-51 187
Msa0223130 MtrunA17_Chr1g0159461 28.726 369 257 5 289 653 225 591 3.96e-50 184
Msa0223130 MtrunA17_Chr8g0376381 31.467 375 237 9 283 653 163 521 1.33e-48 178
Msa0223130 MtrunA17_Chr3g0101141 29.491 373 257 5 285 653 171 541 1.51e-48 178
Msa0223130 MtrunA17_Chr5g0445331 30.159 378 252 8 285 653 157 531 2.73e-48 177
Msa0223130 MtrunA17_Chr8g0357601 29.412 391 247 6 283 656 176 554 3.85e-47 175
Msa0223130 MtrunA17_Chr4g0057891 28.462 390 251 7 281 653 141 519 1.02e-46 173
Msa0223130 MtrunA17_Chr3g0125811 27.913 369 260 5 289 653 203 569 3.77e-46 172
Msa0223130 MtrunA17_Chr2g0306641 30.479 397 252 11 269 653 102 486 1.24e-45 169
Msa0223130 MtrunA17_Chr3g0110971 29.381 388 243 9 283 653 213 586 6.28e-45 169
Msa0223130 MtrunA17_Chr7g0245601 29.428 367 242 7 292 655 441 793 5.90e-43 166
Msa0223130 MtrunA17_Chr4g0038851 26.230 427 247 7 289 653 48 468 8.06e-40 152
Msa0223130 MtrunA17_Chr3g0085681 26.859 417 245 7 290 653 26 435 1.63e-39 150
Msa0223130 MtrunA17_Chr4g0038761 27.926 376 242 8 292 656 87 444 8.16e-39 149
Msa0223130 MtrunA17_Chr2g0294461 28.645 391 247 8 284 653 205 584 8.25e-37 145
Msa0223130 MtrunA17_Chr4g0057291 23.969 388 270 9 283 655 286 663 3.38e-35 141
Msa0223130 MtrunA17_Chr4g0039661 25.464 377 260 7 286 653 190 554 1.25e-31 129
Msa0223130 MtrunA17_Chr1g0200401 26.263 396 247 12 289 653 77 458 2.61e-31 127
Msa0223130 MtrunA17_Chr2g0294471 26.753 385 259 8 284 653 199 575 3.84e-31 128
Msa0223130 MtrunA17_Chr7g0270031 26.842 380 258 9 284 653 184 553 1.32e-29 124
Msa0223130 MtrunA17_Chr1g0183641 26.247 381 250 13 283 653 117 476 2.78e-29 122
Msa0223130 MtrunA17_Chr5g0401131 25.000 384 265 8 289 655 114 491 9.16e-29 120
Msa0223130 MtrunA17_Chr7g0236851 28.938 273 181 6 383 653 395 656 5.09e-26 113
Msa0223130 MtrunA17_Chr5g0424131 25.627 359 237 10 316 654 151 499 3.41e-25 109
Msa0223130 MtrunA17_Chr5g0396491 31.579 209 133 2 289 493 56 258 8.71e-25 108
Msa0223130 MtrunA17_Chr5g0396491 29.530 149 105 0 505 653 329 477 3.46e-16 81.6
Msa0223130 MtrunA17_Chr3g0085361 25.439 342 198 5 361 653 16 349 3.34e-24 104
Msa0223130 MtrunA17_Chr3g0114841 26.685 371 231 13 308 653 145 499 7.63e-24 105
Msa0223130 MtrunA17_Chr8g0383981 24.538 379 246 16 294 656 144 498 1.18e-23 105
Msa0223130 MtrunA17_Chr1g0194621 22.938 388 270 9 284 653 141 517 2.54e-23 104
Msa0223130 MtrunA17_Chr6g0468561 26.087 345 226 10 324 653 331 661 5.75e-23 103
Msa0223130 MtrunA17_Chr5g0401421 21.780 427 280 10 279 656 27 448 8.14e-22 99.0
Msa0223130 MtrunA17_Chr2g0329591 26.593 361 221 11 289 623 74 416 3.55e-20 94.0
Msa0223130 MtrunA17_Chr4g0053901 21.635 416 274 8 289 656 59 470 5.40e-20 93.6
Msa0223130 MtrunA17_Chr7g0230731 23.592 373 258 10 290 653 373 727 8.78e-18 87.8
Msa0223130 MtrunA17_Chr4g0012071 21.939 392 274 10 275 653 178 550 1.71e-17 86.3
Msa0223130 MtrunA17_Chr7g0230771 22.911 371 263 9 290 653 376 730 1.96e-17 86.7
Msa0223130 MtrunA17_Chr8g0344101 24.750 400 243 16 289 653 223 599 2.18e-15 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0223130 AT2G37650.1 43.256 645 320 12 50 655 77 714 1.70e-166 494
Msa0223130 AT2G37650.2 43.256 645 320 12 50 655 65 702 2.41e-166 493
Msa0223130 AT2G29060.2 43.038 632 303 14 50 655 56 656 1.01e-164 487
Msa0223130 AT2G29060.1 42.746 641 329 12 50 655 56 693 2.68e-162 482
Msa0223130 AT5G59450.1 49.263 475 224 7 193 656 132 600 6.14e-158 468
Msa0223130 AT1G07530.1 49.573 468 221 5 201 656 303 767 2.18e-155 467
Msa0223130 AT3G46600.3 50.557 449 199 5 216 656 116 549 2.18e-149 444
Msa0223130 AT3G46600.1 50.557 449 199 5 216 656 148 581 3.97e-149 445
Msa0223130 AT3G46600.2 55.405 370 163 1 289 656 4 373 4.78e-147 432
Msa0223130 AT2G29065.1 41.707 621 323 12 51 656 34 630 1.97e-143 432
Msa0223130 AT1G07520.2 45.021 482 247 6 185 656 58 531 2.28e-136 410
Msa0223130 AT1G07520.4 45.021 482 247 6 185 656 58 531 2.28e-136 410
Msa0223130 AT1G07520.1 38.975 644 339 14 52 656 67 695 2.78e-136 415
Msa0223130 AT1G07520.3 38.975 644 339 14 52 656 68 696 3.22e-136 415
Msa0223130 AT1G50600.3 34.759 374 236 6 285 653 155 525 5.10e-60 210
Msa0223130 AT1G50600.1 34.759 374 236 6 285 653 226 596 2.53e-59 210
Msa0223130 AT1G50600.2 34.759 374 236 6 285 653 230 600 2.62e-59 210
Msa0223130 AT5G48150.1 32.292 384 251 7 276 653 109 489 2.48e-55 196
Msa0223130 AT5G48150.4 32.292 384 251 7 276 653 109 489 2.48e-55 196
Msa0223130 AT5G48150.2 32.292 384 251 7 276 653 109 489 2.48e-55 196
Msa0223130 AT5G48150.3 32.292 384 251 7 276 653 109 489 2.48e-55 196
Msa0223130 AT2G04890.1 30.027 373 248 6 285 653 49 412 1.37e-50 181
Msa0223130 AT1G21450.1 28.455 369 259 4 289 653 225 592 1.67e-47 177
Msa0223130 AT4G17230.1 30.053 376 257 5 284 655 153 526 6.54e-47 174
Msa0223130 AT4G17230.2 30.053 376 257 5 284 655 153 526 6.54e-47 174
Msa0223130 AT1G14920.1 30.400 375 245 9 283 653 166 528 2.91e-45 169
Msa0223130 AT3G54220.1 30.295 373 234 9 292 655 296 651 4.42e-44 168
Msa0223130 AT3G03450.1 29.306 389 236 9 283 653 177 544 2.04e-43 164
Msa0223130 AT2G01570.1 28.533 375 252 9 283 653 218 580 7.78e-42 160
Msa0223130 AT1G66350.1 29.894 378 237 10 283 653 149 505 1.05e-41 159
Msa0223130 AT5G66770.1 28.686 373 255 7 286 653 217 583 2.51e-41 159
Msa0223130 AT5G17490.1 29.365 378 244 9 283 653 154 515 1.63e-40 155
Msa0223130 AT1G50420.1 25.522 431 252 7 286 653 54 478 2.58e-39 151
Msa0223130 AT5G41920.1 27.013 385 253 7 279 653 33 399 6.03e-38 146
Msa0223130 AT3G50650.1 26.133 375 260 7 290 653 173 541 7.55e-36 142
Msa0223130 AT1G63100.1 25.723 346 240 6 285 622 248 584 8.14e-33 134
Msa0223130 AT1G63100.2 25.723 346 240 6 285 622 273 609 1.07e-32 134
Msa0223130 AT4G37650.1 24.814 403 251 11 288 655 145 530 4.51e-32 131
Msa0223130 AT5G52510.1 26.190 378 254 9 292 653 271 639 1.82e-30 127
Msa0223130 AT3G49950.1 27.041 392 249 11 289 655 30 409 6.88e-28 117
Msa0223130 AT4G08250.1 28.022 364 235 13 305 653 125 476 1.70e-27 117
Msa0223130 AT1G55580.1 22.997 387 232 8 318 653 73 444 8.65e-22 99.4
Msa0223130 AT3G13840.1 24.289 387 246 14 289 653 148 509 3.33e-19 91.7

Find 134 sgRNAs with CRISPR-Local

Find 318 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAGTTCCATTGTGAAAA+TGG 0.175227 2_1:+72659407 None:intergenic
CTCACAAGCTATAACATTTA+TGG 0.192701 2_1:+72658205 None:intergenic
CATCGACTACAAAATCTTTA+TGG 0.219672 2_1:+72658051 None:intergenic
CCATCTTCACCTTCTTCTTC+TGG 0.264392 2_1:-72659838 Msa0223130:CDS
AGCGTTCATGTTATTGATTT+TGG 0.271020 2_1:-72658758 Msa0223130:CDS
CCTGACCTGCCACTGCTTGT+AGG 0.274499 2_1:+72658154 None:intergenic
CCTCCAAAGCTTCGAATTAC+AGG 0.281535 2_1:-72658674 Msa0223130:CDS
TATGTATCTTAGGATGTTAT+TGG 0.287625 2_1:+72659774 None:intergenic
GTTACTTGGTTTACTGAGTT+TGG 0.288731 2_1:+72659886 None:intergenic
AGGCAACACTCTTCGCCTTT+TGG 0.309946 2_1:-72658989 Msa0223130:CDS
ACTCCATGGAAGAAGACTTT+TGG 0.314241 2_1:+72658380 None:intergenic
GGATGTCATTTATGTATCTT+AGG 0.317485 2_1:+72659764 None:intergenic
ACAACTTCATTGCACAGAAA+TGG 0.347339 2_1:-72658550 Msa0223130:CDS
TTTGAATGCTTTATCAGCTT+GGG 0.351490 2_1:-72657988 Msa0223130:CDS
AAGTCCTTCTACGATGTTCT+TGG 0.359023 2_1:-72659667 Msa0223130:CDS
GCAGGAATGTTTAAGACTTC+AGG 0.359559 2_1:-72659698 Msa0223130:CDS
ATTCCTGTAATTCGAAGCTT+TGG 0.359837 2_1:+72658671 None:intergenic
GCATCACATAATGTTGATTC+AGG 0.366614 2_1:-72659163 Msa0223130:CDS
TTAGCTCATTACTTTGCTAA+TGG 0.370579 2_1:-72658950 Msa0223130:CDS
TAAAGGACTTTGTAGTTGTA+TGG 0.371367 2_1:+72659495 None:intergenic
CATTTGGTGAAGGCTGGTTA+TGG 0.381462 2_1:+72659644 None:intergenic
AAATCTTGCAGAAAAGTGTT+TGG 0.386250 2_1:+72659916 None:intergenic
TCTTCTGGCACAAGCTCAAA+TGG 0.386431 2_1:-72659823 Msa0223130:CDS
AAGATGTGTTGAAGCTGATC+AGG 0.390657 2_1:-72658412 Msa0223130:CDS
CCTCTTGATGTGGCAACTGC+TGG 0.394427 2_1:-72658887 Msa0223130:CDS
TTCTGACGAGGCACGGCCAC+TGG 0.399566 2_1:+72658720 None:intergenic
TCAGTAAACCAAGTAACAAA+TGG 0.401437 2_1:-72659880 Msa0223130:CDS
CCTTGTGTCTCGATGACAAC+TGG 0.405616 2_1:+72659436 None:intergenic
ATTCAAACCCATTTGTTACT+TGG 0.405951 2_1:+72659872 None:intergenic
CCTACAAGCAGTGGCAGGTC+AGG 0.408410 2_1:-72658154 Msa0223130:CDS
AGCTTCAACACATCTTCTCT+TGG 0.409629 2_1:+72658419 None:intergenic
GAGCTGGGTTCAAGCAAATC+AGG 0.413441 2_1:-72658124 Msa0223130:CDS
ATGCTTGAGAGAGGGTTGTT+TGG 0.418474 2_1:-72658236 Msa0223130:CDS
GTTTCCTCCACCCTTTCTGC+AGG 0.420825 2_1:+72658623 None:intergenic
CAACATTATGTGATGCTTGT+TGG 0.426130 2_1:+72659170 None:intergenic
CAACTTCATTGCACAGAAAT+GGG 0.427023 2_1:-72658549 Msa0223130:CDS
TGCCACTGCTTGTAGGTCTC+AGG 0.429715 2_1:+72658161 None:intergenic
ATCGTTGTTTGATATGTTTG+AGG 0.437931 2_1:-72658291 Msa0223130:CDS
ATGTTATTGGAATATCTTGT+CGG 0.439317 2_1:+72659787 None:intergenic
TTCAATGCCACAGTTTCATC+AGG 0.440053 2_1:+72658446 None:intergenic
TCTGGGTCAGTGCTATCATT+TGG 0.448392 2_1:+72659628 None:intergenic
GCGCGATCGAAATTTCCATC+TGG 0.453753 2_1:+72659610 None:intergenic
TGCTATCATTTGGTGAAGGC+TGG 0.460261 2_1:+72659638 None:intergenic
ATGCCGCGGCAGTGGATTTG+TGG 0.465356 2_1:-72659087 Msa0223130:CDS
CATGGATGAAGAAGATGACT+TGG 0.465816 2_1:-72659740 Msa0223130:CDS
GCAGAAAGGGTGGAGGAAAC+CGG 0.469253 2_1:-72658620 Msa0223130:CDS
AAGCTCAAATGGACAATCCT+TGG 0.473031 2_1:-72659812 Msa0223130:CDS
ATGGCCAAGAACATCGTAGA+AGG 0.479055 2_1:+72659663 None:intergenic
TTCACAATGGAACTTGGAGT+TGG 0.481843 2_1:-72659402 Msa0223130:CDS
AGTACCTCATCTAAAATCTC+TGG 0.483079 2_1:+72659226 None:intergenic
AGCTTGTGAGGGTGCAGAAA+GGG 0.483774 2_1:-72658192 Msa0223130:CDS
TAGTCGATGTGGATGGTAAG+TGG 0.492425 2_1:-72658037 Msa0223130:CDS
GGAATTGAGCTTCCTCGGCC+AGG 0.495711 2_1:-72658653 Msa0223130:CDS
CGCTGGAGTCCATCACCAAA+AGG 0.496232 2_1:+72658974 None:intergenic
AAAACTTCTTCCACCGTCAC+TGG 0.496341 2_1:+72659367 None:intergenic
CGAAGCTTTGGAGGACCACC+AGG 0.497005 2_1:+72658683 None:intergenic
CCTGTAATTCGAAGCTTTGG+AGG 0.497546 2_1:+72658674 None:intergenic
AATCCAAAAGTCTTCTTCCA+TGG 0.502095 2_1:-72658383 Msa0223130:CDS
TAATGGTCTTGAGATAAGAT+TGG 0.503318 2_1:-72658933 Msa0223130:CDS
CTTTCTGCAGGTCTAAATCC+TGG 0.504296 2_1:+72658635 None:intergenic
GACAAACAATGCCGCGGCAG+TGG 0.508260 2_1:-72659095 Msa0223130:CDS
GTGCTTCCTTAAACCGAGTC+GGG 0.509273 2_1:+72658327 None:intergenic
TTCTTCCATGGAGTTGCCAA+TGG 0.510258 2_1:-72658371 Msa0223130:CDS
AACGAAAACAATCGTGAAGA+AGG 0.511353 2_1:-72658078 Msa0223130:CDS
ATGAGCTACTCAAACAGATT+AGG 0.511922 2_1:-72659009 Msa0223130:CDS
CAGTAAACCAAGTAACAAAT+GGG 0.514266 2_1:-72659879 Msa0223130:CDS
CTTTACATCCTCGAGGCAGA+TGG 0.514553 2_1:+72658523 None:intergenic
TTACAGGAATTGAGCTTCCT+CGG 0.515630 2_1:-72658658 Msa0223130:CDS
CAGGTCAGGAATAAGAGAGC+TGG 0.517229 2_1:-72658140 Msa0223130:CDS
CGCGATCGAAATTTCCATCT+GGG 0.519839 2_1:+72659611 None:intergenic
AGGATTTAGACCTGCAGAAA+GGG 0.522516 2_1:-72658633 Msa0223130:CDS
TCGAGGATGTAAAGATAGAC+AGG 0.523675 2_1:-72658514 Msa0223130:CDS
GGAATATCTTGTCGGTTCCA+AGG 0.526658 2_1:+72659795 None:intergenic
GAGCTTGTGCCAGAAGAAGA+AGG 0.528577 2_1:+72659829 None:intergenic
AAACATTCCTGCAGCATGCA+AGG 0.535746 2_1:+72659709 None:intergenic
CTGTGGCGAGCTACGATCAA+AGG 0.536487 2_1:-72659039 Msa0223130:CDS
GTAGTTTGCTAGACGCCGTC+CGG 0.538457 2_1:+72658601 None:intergenic
CAGGATTTAGACCTGCAGAA+AGG 0.540009 2_1:-72658634 Msa0223130:CDS
AACTTCCATTGGCAACTCCA+TGG 0.540581 2_1:+72658366 None:intergenic
GCAATGAAGTTGTATTCAAA+TGG 0.550757 2_1:+72658560 None:intergenic
AGTCGATGTGGATGGTAAGT+GGG 0.556259 2_1:-72658036 Msa0223130:CDS
CGGTGCGCTATAACTTCCAT+TGG 0.556645 2_1:+72658355 None:intergenic
TAATGTTGATTCAGGAGGAA+AGG 0.556753 2_1:-72659155 Msa0223130:CDS
TTGTGCACATTGATTTAGCA+TGG 0.558170 2_1:+72659063 None:intergenic
CCAGAAGAAGAAGGTGAAGA+TGG 0.558793 2_1:+72659838 None:intergenic
ATGCCACAGTTTCATCAGGA+AGG 0.560068 2_1:+72658450 None:intergenic
CCAGCAGTTGCCACATCAAG+AGG 0.562934 2_1:+72658887 None:intergenic
TGAGACCTACAAGCAGTGGC+AGG 0.569446 2_1:-72658159 Msa0223130:CDS
TAACTCATATGAATCTAACT+TGG 0.569816 2_1:-72659548 Msa0223130:CDS
ATACATAAATGACATCCTCA+TGG 0.570653 2_1:-72659758 Msa0223130:CDS
CATAAATGTTATAGCTTGTG+AGG 0.573605 2_1:-72658204 Msa0223130:CDS
AGGTCAGGAATAAGAGAGCT+GGG 0.580907 2_1:-72658139 Msa0223130:CDS
ACATCAAGAGGCTGATATGA+TGG 0.581066 2_1:+72658899 None:intergenic
TGTAATGAACAAGAGGGAAG+AGG 0.582616 2_1:-72659289 Msa0223130:CDS
TATCAAACAACGATGAGAAG+TGG 0.582630 2_1:+72658300 None:intergenic
CACTCTCTGCAAAGGAGACG+CGG 0.582762 2_1:+72658832 None:intergenic
CAGAGATCGATGCTTGAGAG+AGG 0.587984 2_1:-72658245 Msa0223130:CDS
CAAAGTAATGAGCTAAACGC+TGG 0.593615 2_1:+72658957 None:intergenic
TGATACTTGTAATGAACAAG+AGG 0.595387 2_1:-72659296 Msa0223130:CDS
GAGGTACTGCTTTATCAGAA+AGG 0.596446 2_1:-72659211 Msa0223130:CDS
ATCATATCAGCCTCTTGATG+TGG 0.597759 2_1:-72658897 Msa0223130:CDS
GTATTTGTCACTCTCTGCAA+AGG 0.601498 2_1:+72658824 None:intergenic
ATTTAGACCTGCAGAAAGGG+TGG 0.601556 2_1:-72658630 Msa0223130:CDS
AGGGTGCAGAAAGGGTTGAA+AGG 0.602354 2_1:-72658184 Msa0223130:CDS
GATAGTCTTCTGACGAGGCA+CGG 0.602753 2_1:+72658713 None:intergenic
ACCTCATCTAAAATCTCTGG+TGG 0.604084 2_1:+72659229 None:intergenic
AGTGCTTCCTTAAACCGAGT+CGG 0.604179 2_1:+72658326 None:intergenic
GCAGGTCTAAATCCTGGCCG+AGG 0.605176 2_1:+72658641 None:intergenic
TAGCTTGTGAGGGTGCAGAA+AGG 0.607236 2_1:-72658193 Msa0223130:CDS
AATGATAGCACTGACCCAGA+TGG 0.609483 2_1:-72659625 Msa0223130:CDS
AAGATTCGAATTCGACGACA+TGG 0.612871 2_1:+72659521 None:intergenic
CCAGTTGTCATCGAGACACA+AGG 0.613282 2_1:-72659436 Msa0223130:CDS
TGGGTTCAAGCAAATCAGGT+TGG 0.613823 2_1:-72658120 Msa0223130:CDS
TAGACCTGCAGAAAGGGTGG+AGG 0.621359 2_1:-72658627 Msa0223130:CDS
GGCCTGAGACCTACAAGCAG+TGG 0.622095 2_1:-72658163 Msa0223130:CDS
GATACTTGTAATGAACAAGA+GGG 0.631778 2_1:-72659295 Msa0223130:CDS
TCAGTGCTATCATTTGGTGA+AGG 0.638613 2_1:+72659634 None:intergenic
TGACATTCAGCCAGTGACGG+TGG 0.640113 2_1:-72659377 Msa0223130:CDS
TCACATAATGTTGATTCAGG+AGG 0.642164 2_1:-72659160 Msa0223130:CDS
GGTCCACAAATCCACTGCCG+CGG 0.645648 2_1:+72659084 None:intergenic
GAATGACATTCAGCCAGTGA+CGG 0.650343 2_1:-72659380 Msa0223130:CDS
AGAGATCGATGCTTGAGAGA+GGG 0.659674 2_1:-72658244 Msa0223130:CDS
GAACCTTCCTGATGAAACTG+TGG 0.660816 2_1:-72658453 Msa0223130:CDS
AAATCAATGTGCACAAGCTG+TGG 0.661819 2_1:-72659056 Msa0223130:CDS
TGTCTATCTTTACATCCTCG+AGG 0.665186 2_1:+72658516 None:intergenic
AGACTATCATTAAGACCTGG+TGG 0.687440 2_1:-72658698 Msa0223130:CDS
AGTCATCTTCTTCATCCATG+AGG 0.688746 2_1:+72659743 None:intergenic
AAAGGGTGGAGGAAACCGGA+CGG 0.693036 2_1:-72658616 Msa0223130:CDS
AGAAGACTATCATTAAGACC+TGG 0.694335 2_1:-72658701 Msa0223130:CDS
ATAAATGTTATAGCTTGTGA+GGG 0.694950 2_1:-72658203 Msa0223130:CDS
ATGGGAAACCATCTGCCTCG+AGG 0.697664 2_1:-72658531 Msa0223130:CDS
ATCTACGACAAACAATGCCG+CGG 0.702968 2_1:-72659101 Msa0223130:CDS
GATCTCTGCATGTCTTCACG+AGG 0.714627 2_1:+72658260 None:intergenic
TTAATGATAGTCTTCTGACG+AGG 0.747045 2_1:+72658708 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTAGTTTTTTTCTATTAA+AGG + chr2_1:72658427-72658446 None:intergenic 15.0%
!!! TAGTAGTTTTTTTCTATTAA+AGG + chr2_1:72658427-72658446 None:intergenic 15.0%
!!! ATTGATTTTGGAATTTTCTA+TGG - chr2_1:72659156-72659175 Msa0223130:CDS 20.0%
!!! ATTGATTTTGGAATTTTCTA+TGG - chr2_1:72659156-72659175 Msa0223130:CDS 20.0%
! ATGTTATTGGAATATCTTGT+CGG + chr2_1:72658118-72658137 None:intergenic 25.0%
! TATGTATCTTAGGATGTTAT+TGG + chr2_1:72658131-72658150 None:intergenic 25.0%
! TAACTCATATGAATCTAACT+TGG - chr2_1:72658354-72658373 Msa0223130:CDS 25.0%
!!! CACTATTTTGAAAATCGTTT+TGG + chr2_1:72659112-72659131 None:intergenic 25.0%
!! ATAAATGTTATAGCTTGTGA+GGG - chr2_1:72659699-72659718 Msa0223130:CDS 25.0%
! ATGTTATTGGAATATCTTGT+CGG + chr2_1:72658118-72658137 None:intergenic 25.0%
! TATGTATCTTAGGATGTTAT+TGG + chr2_1:72658131-72658150 None:intergenic 25.0%
! TAACTCATATGAATCTAACT+TGG - chr2_1:72658354-72658373 Msa0223130:CDS 25.0%
!!! CACTATTTTGAAAATCGTTT+TGG + chr2_1:72659112-72659131 None:intergenic 25.0%
!! ATAAATGTTATAGCTTGTGA+GGG - chr2_1:72659699-72659718 Msa0223130:CDS 25.0%
!! AAATCTTGCAGAAAAGTGTT+TGG + chr2_1:72657989-72658008 None:intergenic 30.0%
TCAGTAAACCAAGTAACAAA+TGG - chr2_1:72658022-72658041 Msa0223130:CDS 30.0%
CAGTAAACCAAGTAACAAAT+GGG - chr2_1:72658023-72658042 Msa0223130:CDS 30.0%
! ATTCAAACCCATTTGTTACT+TGG + chr2_1:72658033-72658052 None:intergenic 30.0%
! GGATGTCATTTATGTATCTT+AGG + chr2_1:72658141-72658160 None:intergenic 30.0%
ATACATAAATGACATCCTCA+TGG - chr2_1:72658144-72658163 Msa0223130:CDS 30.0%
TAAAGGACTTTGTAGTTGTA+TGG + chr2_1:72658410-72658429 None:intergenic 30.0%
!!! ATCTTACGACTTCTTTTTTC+TGG + chr2_1:72658574-72658593 None:intergenic 30.0%
AGAAAAAAGAAGTCGTAAGA+TGG - chr2_1:72658573-72658592 Msa0223130:CDS 30.0%
TGATACTTGTAATGAACAAG+AGG - chr2_1:72658606-72658625 Msa0223130:CDS 30.0%
GATACTTGTAATGAACAAGA+GGG - chr2_1:72658607-72658626 Msa0223130:CDS 30.0%
TTAGCTCATTACTTTGCTAA+TGG - chr2_1:72658952-72658971 Msa0223130:CDS 30.0%
! TAATGGTCTTGAGATAAGAT+TGG - chr2_1:72658969-72658988 Msa0223130:CDS 30.0%
!!! AGCGTTCATGTTATTGATTT+TGG - chr2_1:72659144-72659163 Msa0223130:CDS 30.0%
GCAATGAAGTTGTATTCAAA+TGG + chr2_1:72659345-72659364 None:intergenic 30.0%
ATCGTTGTTTGATATGTTTG+AGG - chr2_1:72659611-72659630 Msa0223130:CDS 30.0%
CTCACAAGCTATAACATTTA+TGG + chr2_1:72659700-72659719 None:intergenic 30.0%
! CATAAATGTTATAGCTTGTG+AGG - chr2_1:72659698-72659717 Msa0223130:CDS 30.0%
! CATCGACTACAAAATCTTTA+TGG + chr2_1:72659854-72659873 None:intergenic 30.0%
! TAAAGATTTTGTAGTCGATG+TGG - chr2_1:72659854-72659873 Msa0223130:CDS 30.0%
! TTTTGAATGCTTTATCAGCT+TGG - chr2_1:72659913-72659932 Msa0223130:CDS 30.0%
TTTGAATGCTTTATCAGCTT+GGG - chr2_1:72659914-72659933 Msa0223130:CDS 30.0%
!! AAATCTTGCAGAAAAGTGTT+TGG + chr2_1:72657989-72658008 None:intergenic 30.0%
TCAGTAAACCAAGTAACAAA+TGG - chr2_1:72658022-72658041 Msa0223130:CDS 30.0%
CAGTAAACCAAGTAACAAAT+GGG - chr2_1:72658023-72658042 Msa0223130:CDS 30.0%
! ATTCAAACCCATTTGTTACT+TGG + chr2_1:72658033-72658052 None:intergenic 30.0%
! GGATGTCATTTATGTATCTT+AGG + chr2_1:72658141-72658160 None:intergenic 30.0%
ATACATAAATGACATCCTCA+TGG - chr2_1:72658144-72658163 Msa0223130:CDS 30.0%
TAAAGGACTTTGTAGTTGTA+TGG + chr2_1:72658410-72658429 None:intergenic 30.0%
!!! ATCTTACGACTTCTTTTTTC+TGG + chr2_1:72658574-72658593 None:intergenic 30.0%
AGAAAAAAGAAGTCGTAAGA+TGG - chr2_1:72658573-72658592 Msa0223130:CDS 30.0%
TGATACTTGTAATGAACAAG+AGG - chr2_1:72658606-72658625 Msa0223130:CDS 30.0%
GATACTTGTAATGAACAAGA+GGG - chr2_1:72658607-72658626 Msa0223130:CDS 30.0%
TTAGCTCATTACTTTGCTAA+TGG - chr2_1:72658952-72658971 Msa0223130:CDS 30.0%
! TAATGGTCTTGAGATAAGAT+TGG - chr2_1:72658969-72658988 Msa0223130:CDS 30.0%
!!! AGCGTTCATGTTATTGATTT+TGG - chr2_1:72659144-72659163 Msa0223130:CDS 30.0%
GCAATGAAGTTGTATTCAAA+TGG + chr2_1:72659345-72659364 None:intergenic 30.0%
ATCGTTGTTTGATATGTTTG+AGG - chr2_1:72659611-72659630 Msa0223130:CDS 30.0%
CTCACAAGCTATAACATTTA+TGG + chr2_1:72659700-72659719 None:intergenic 30.0%
! CATAAATGTTATAGCTTGTG+AGG - chr2_1:72659698-72659717 Msa0223130:CDS 30.0%
! CATCGACTACAAAATCTTTA+TGG + chr2_1:72659854-72659873 None:intergenic 30.0%
! TAAAGATTTTGTAGTCGATG+TGG - chr2_1:72659854-72659873 Msa0223130:CDS 30.0%
! TTTTGAATGCTTTATCAGCT+TGG - chr2_1:72659913-72659932 Msa0223130:CDS 30.0%
TTTGAATGCTTTATCAGCTT+GGG - chr2_1:72659914-72659933 Msa0223130:CDS 30.0%
GTTACTTGGTTTACTGAGTT+TGG + chr2_1:72658019-72658038 None:intergenic 35.0%
! ACCATTTTCACAATGGAACT+TGG - chr2_1:72658494-72658513 Msa0223130:CDS 35.0%
TCCAAGTTCCATTGTGAAAA+TGG + chr2_1:72658498-72658517 None:intergenic 35.0%
!!! CTTTTTTCTGGTTCTGTTGA+AGG + chr2_1:72658562-72658581 None:intergenic 35.0%
! AGTACCTCATCTAAAATCTC+TGG + chr2_1:72658679-72658698 None:intergenic 35.0%
CAACATTATGTGATGCTTGT+TGG + chr2_1:72658735-72658754 None:intergenic 35.0%
!! GCATCACATAATGTTGATTC+AGG - chr2_1:72658739-72658758 Msa0223130:CDS 35.0%
!! TCACATAATGTTGATTCAGG+AGG - chr2_1:72658742-72658761 Msa0223130:CDS 35.0%
!! TAATGTTGATTCAGGAGGAA+AGG - chr2_1:72658747-72658766 Msa0223130:CDS 35.0%
!! TTGTGCACATTGATTTAGCA+TGG + chr2_1:72658842-72658861 None:intergenic 35.0%
ATGAGCTACTCAAACAGATT+AGG - chr2_1:72658893-72658912 Msa0223130:CDS 35.0%
! GAATTTTCTATGGTTTCCAG+TGG - chr2_1:72659166-72659185 Msa0223130:CDS 35.0%
TTAATGATAGTCTTCTGACG+AGG + chr2_1:72659197-72659216 None:intergenic 35.0%
AGAAGACTATCATTAAGACC+TGG - chr2_1:72659201-72659220 Msa0223130:CDS 35.0%
ATTCCTGTAATTCGAAGCTT+TGG + chr2_1:72659234-72659253 None:intergenic 35.0%
ACAACTTCATTGCACAGAAA+TGG - chr2_1:72659352-72659371 Msa0223130:CDS 35.0%
CAACTTCATTGCACAGAAAT+GGG - chr2_1:72659353-72659372 Msa0223130:CDS 35.0%
AATCCAAAAGTCTTCTTCCA+TGG - chr2_1:72659519-72659538 Msa0223130:CDS 35.0%
TATCAAACAACGATGAGAAG+TGG + chr2_1:72659605-72659624 None:intergenic 35.0%
AACGAAAACAATCGTGAAGA+AGG - chr2_1:72659824-72659843 Msa0223130:CDS 35.0%
GTTACTTGGTTTACTGAGTT+TGG + chr2_1:72658019-72658038 None:intergenic 35.0%
! ACCATTTTCACAATGGAACT+TGG - chr2_1:72658494-72658513 Msa0223130:CDS 35.0%
TCCAAGTTCCATTGTGAAAA+TGG + chr2_1:72658498-72658517 None:intergenic 35.0%
!!! CTTTTTTCTGGTTCTGTTGA+AGG + chr2_1:72658562-72658581 None:intergenic 35.0%
! AGTACCTCATCTAAAATCTC+TGG + chr2_1:72658679-72658698 None:intergenic 35.0%
CAACATTATGTGATGCTTGT+TGG + chr2_1:72658735-72658754 None:intergenic 35.0%
!! GCATCACATAATGTTGATTC+AGG - chr2_1:72658739-72658758 Msa0223130:CDS 35.0%
!! TCACATAATGTTGATTCAGG+AGG - chr2_1:72658742-72658761 Msa0223130:CDS 35.0%
!! TAATGTTGATTCAGGAGGAA+AGG - chr2_1:72658747-72658766 Msa0223130:CDS 35.0%
!! TTGTGCACATTGATTTAGCA+TGG + chr2_1:72658842-72658861 None:intergenic 35.0%
ATGAGCTACTCAAACAGATT+AGG - chr2_1:72658893-72658912 Msa0223130:CDS 35.0%
! GAATTTTCTATGGTTTCCAG+TGG - chr2_1:72659166-72659185 Msa0223130:CDS 35.0%
TTAATGATAGTCTTCTGACG+AGG + chr2_1:72659197-72659216 None:intergenic 35.0%
AGAAGACTATCATTAAGACC+TGG - chr2_1:72659201-72659220 Msa0223130:CDS 35.0%
ATTCCTGTAATTCGAAGCTT+TGG + chr2_1:72659234-72659253 None:intergenic 35.0%
ACAACTTCATTGCACAGAAA+TGG - chr2_1:72659352-72659371 Msa0223130:CDS 35.0%
CAACTTCATTGCACAGAAAT+GGG - chr2_1:72659353-72659372 Msa0223130:CDS 35.0%
AATCCAAAAGTCTTCTTCCA+TGG - chr2_1:72659519-72659538 Msa0223130:CDS 35.0%
TATCAAACAACGATGAGAAG+TGG + chr2_1:72659605-72659624 None:intergenic 35.0%
AACGAAAACAATCGTGAAGA+AGG - chr2_1:72659824-72659843 Msa0223130:CDS 35.0%
AAGCTCAAATGGACAATCCT+TGG - chr2_1:72658090-72658109 Msa0223130:CDS 40.0%
AGTCATCTTCTTCATCCATG+AGG + chr2_1:72658162-72658181 None:intergenic 40.0%
CATGGATGAAGAAGATGACT+TGG - chr2_1:72658162-72658181 Msa0223130:CDS 40.0%
! GCAGGAATGTTTAAGACTTC+AGG - chr2_1:72658204-72658223 Msa0223130:CDS 40.0%
! ATCGTAGAAGGACTTTTCAG+CGG + chr2_1:72658230-72658249 None:intergenic 40.0%
AAGTCCTTCTACGATGTTCT+TGG - chr2_1:72658235-72658254 Msa0223130:CDS 40.0%
! TCAGTGCTATCATTTGGTGA+AGG + chr2_1:72658271-72658290 None:intergenic 40.0%
AAGATTCGAATTCGACGACA+TGG + chr2_1:72658384-72658403 None:intergenic 40.0%
! GGTGCTAACCATTTTCACAA+TGG - chr2_1:72658487-72658506 Msa0223130:CDS 40.0%
TTCACAATGGAACTTGGAGT+TGG - chr2_1:72658500-72658519 Msa0223130:CDS 40.0%
TGTAATGAACAAGAGGGAAG+AGG - chr2_1:72658613-72658632 Msa0223130:CDS 40.0%
! ACCTCATCTAAAATCTCTGG+TGG + chr2_1:72658676-72658695 None:intergenic 40.0%
!! GAGGTACTGCTTTATCAGAA+AGG - chr2_1:72658691-72658710 Msa0223130:CDS 40.0%
AAATCAATGTGCACAAGCTG+TGG - chr2_1:72658846-72658865 Msa0223130:CDS 40.0%
CAAAGTAATGAGCTAAACGC+TGG + chr2_1:72658948-72658967 None:intergenic 40.0%
ACATCAAGAGGCTGATATGA+TGG + chr2_1:72659006-72659025 None:intergenic 40.0%
ATCATATCAGCCTCTTGATG+TGG - chr2_1:72659005-72659024 Msa0223130:CDS 40.0%
GTATTTGTCACTCTCTGCAA+AGG + chr2_1:72659081-72659100 None:intergenic 40.0%
AGACTATCATTAAGACCTGG+TGG - chr2_1:72659204-72659223 Msa0223130:CDS 40.0%
TTACAGGAATTGAGCTTCCT+CGG - chr2_1:72659244-72659263 Msa0223130:CDS 40.0%
AGGATTTAGACCTGCAGAAA+GGG - chr2_1:72659269-72659288 Msa0223130:CDS 40.0%
TGTCTATCTTTACATCCTCG+AGG + chr2_1:72659389-72659408 None:intergenic 40.0%
TCGAGGATGTAAAGATAGAC+AGG - chr2_1:72659388-72659407 Msa0223130:CDS 40.0%
TTCAATGCCACAGTTTCATC+AGG + chr2_1:72659459-72659478 None:intergenic 40.0%
AGCTTCAACACATCTTCTCT+TGG + chr2_1:72659486-72659505 None:intergenic 40.0%
AAGATGTGTTGAAGCTGATC+AGG - chr2_1:72659490-72659509 Msa0223130:CDS 40.0%
! ACTCCATGGAAGAAGACTTT+TGG + chr2_1:72659525-72659544 None:intergenic 40.0%
TTAAACCGAGTCGGGAAAAA+CGG + chr2_1:72659570-72659589 None:intergenic 40.0%
! GATTTTGTAGTCGATGTGGA+TGG - chr2_1:72659858-72659877 Msa0223130:CDS 40.0%
AAGCTCAAATGGACAATCCT+TGG - chr2_1:72658090-72658109 Msa0223130:CDS 40.0%
AGTCATCTTCTTCATCCATG+AGG + chr2_1:72658162-72658181 None:intergenic 40.0%
CATGGATGAAGAAGATGACT+TGG - chr2_1:72658162-72658181 Msa0223130:CDS 40.0%
! GCAGGAATGTTTAAGACTTC+AGG - chr2_1:72658204-72658223 Msa0223130:CDS 40.0%
! ATCGTAGAAGGACTTTTCAG+CGG + chr2_1:72658230-72658249 None:intergenic 40.0%
AAGTCCTTCTACGATGTTCT+TGG - chr2_1:72658235-72658254 Msa0223130:CDS 40.0%
! TCAGTGCTATCATTTGGTGA+AGG + chr2_1:72658271-72658290 None:intergenic 40.0%
AAGATTCGAATTCGACGACA+TGG + chr2_1:72658384-72658403 None:intergenic 40.0%
! GGTGCTAACCATTTTCACAA+TGG - chr2_1:72658487-72658506 Msa0223130:CDS 40.0%
TTCACAATGGAACTTGGAGT+TGG - chr2_1:72658500-72658519 Msa0223130:CDS 40.0%
TGTAATGAACAAGAGGGAAG+AGG - chr2_1:72658613-72658632 Msa0223130:CDS 40.0%
! ACCTCATCTAAAATCTCTGG+TGG + chr2_1:72658676-72658695 None:intergenic 40.0%
!! GAGGTACTGCTTTATCAGAA+AGG - chr2_1:72658691-72658710 Msa0223130:CDS 40.0%
AAATCAATGTGCACAAGCTG+TGG - chr2_1:72658846-72658865 Msa0223130:CDS 40.0%
CAAAGTAATGAGCTAAACGC+TGG + chr2_1:72658948-72658967 None:intergenic 40.0%
ACATCAAGAGGCTGATATGA+TGG + chr2_1:72659006-72659025 None:intergenic 40.0%
ATCATATCAGCCTCTTGATG+TGG - chr2_1:72659005-72659024 Msa0223130:CDS 40.0%
GTATTTGTCACTCTCTGCAA+AGG + chr2_1:72659081-72659100 None:intergenic 40.0%
AGACTATCATTAAGACCTGG+TGG - chr2_1:72659204-72659223 Msa0223130:CDS 40.0%
TTACAGGAATTGAGCTTCCT+CGG - chr2_1:72659244-72659263 Msa0223130:CDS 40.0%
AGGATTTAGACCTGCAGAAA+GGG - chr2_1:72659269-72659288 Msa0223130:CDS 40.0%
TGTCTATCTTTACATCCTCG+AGG + chr2_1:72659389-72659408 None:intergenic 40.0%
TCGAGGATGTAAAGATAGAC+AGG - chr2_1:72659388-72659407 Msa0223130:CDS 40.0%
TTCAATGCCACAGTTTCATC+AGG + chr2_1:72659459-72659478 None:intergenic 40.0%
AGCTTCAACACATCTTCTCT+TGG + chr2_1:72659486-72659505 None:intergenic 40.0%
AAGATGTGTTGAAGCTGATC+AGG - chr2_1:72659490-72659509 Msa0223130:CDS 40.0%
! ACTCCATGGAAGAAGACTTT+TGG + chr2_1:72659525-72659544 None:intergenic 40.0%
TTAAACCGAGTCGGGAAAAA+CGG + chr2_1:72659570-72659589 None:intergenic 40.0%
! GATTTTGTAGTCGATGTGGA+TGG - chr2_1:72659858-72659877 Msa0223130:CDS 40.0%
CCAGAAGAAGAAGGTGAAGA+TGG + chr2_1:72658067-72658086 None:intergenic 45.0%
CCATCTTCACCTTCTTCTTC+TGG - chr2_1:72658064-72658083 Msa0223130:CDS 45.0%
TCTTCTGGCACAAGCTCAAA+TGG - chr2_1:72658079-72658098 Msa0223130:CDS 45.0%
!! GGAATATCTTGTCGGTTCCA+AGG + chr2_1:72658110-72658129 None:intergenic 45.0%
AAACATTCCTGCAGCATGCA+AGG + chr2_1:72658196-72658215 None:intergenic 45.0%
ATGGCCAAGAACATCGTAGA+AGG + chr2_1:72658242-72658261 None:intergenic 45.0%
! CATTTGGTGAAGGCTGGTTA+TGG + chr2_1:72658261-72658280 None:intergenic 45.0%
! TGCTATCATTTGGTGAAGGC+TGG + chr2_1:72658267-72658286 None:intergenic 45.0%
TCTGGGTCAGTGCTATCATT+TGG + chr2_1:72658277-72658296 None:intergenic 45.0%
!! AATGATAGCACTGACCCAGA+TGG - chr2_1:72658277-72658296 Msa0223130:CDS 45.0%
CGCGATCGAAATTTCCATCT+GGG + chr2_1:72658294-72658313 None:intergenic 45.0%
!!! GCTGCTAGTTTTGAGAGAAG+TGG - chr2_1:72658313-72658332 Msa0223130:CDS 45.0%
GAATGACATTCAGCCAGTGA+CGG - chr2_1:72658522-72658541 Msa0223130:CDS 45.0%
AAAACTTCTTCCACCGTCAC+TGG + chr2_1:72658538-72658557 None:intergenic 45.0%
! GCCACCAGAGATTTTAGATG+AGG - chr2_1:72658672-72658691 Msa0223130:CDS 45.0%
ATCTACGACAAACAATGCCG+CGG - chr2_1:72658801-72658820 Msa0223130:CDS 45.0%
! CCTGTAATTCGAAGCTTTGG+AGG + chr2_1:72659231-72659250 None:intergenic 45.0%
CCTCCAAAGCTTCGAATTAC+AGG - chr2_1:72659228-72659247 Msa0223130:CDS 45.0%
CTTTCTGCAGGTCTAAATCC+TGG + chr2_1:72659270-72659289 None:intergenic 45.0%
CAGGATTTAGACCTGCAGAA+AGG - chr2_1:72659268-72659287 Msa0223130:CDS 45.0%
ATTTAGACCTGCAGAAAGGG+TGG - chr2_1:72659272-72659291 Msa0223130:CDS 45.0%
GAACCTTCCTGATGAAACTG+TGG - chr2_1:72659449-72659468 Msa0223130:CDS 45.0%
ATGCCACAGTTTCATCAGGA+AGG + chr2_1:72659455-72659474 None:intergenic 45.0%
TTCTTCCATGGAGTTGCCAA+TGG - chr2_1:72659531-72659550 Msa0223130:CDS 45.0%
AACTTCCATTGGCAACTCCA+TGG + chr2_1:72659539-72659558 None:intergenic 45.0%
!!! GTTTTTCCCGACTCGGTTTA+AGG - chr2_1:72659569-72659588 Msa0223130:CDS 45.0%
AGTGCTTCCTTAAACCGAGT+CGG + chr2_1:72659579-72659598 None:intergenic 45.0%
AGAGATCGATGCTTGAGAGA+GGG - chr2_1:72659658-72659677 Msa0223130:CDS 45.0%
ATGCTTGAGAGAGGGTTGTT+TGG - chr2_1:72659666-72659685 Msa0223130:CDS 45.0%
AGGTCAGGAATAAGAGAGCT+GGG - chr2_1:72659763-72659782 Msa0223130:CDS 45.0%
TGGGTTCAAGCAAATCAGGT+TGG - chr2_1:72659782-72659801 Msa0223130:CDS 45.0%
TAGTCGATGTGGATGGTAAG+TGG - chr2_1:72659865-72659884 Msa0223130:CDS 45.0%
AGTCGATGTGGATGGTAAGT+GGG - chr2_1:72659866-72659885 Msa0223130:CDS 45.0%
!! GATGGTAAGTGGGTTTTGCA+GGG - chr2_1:72659876-72659895 Msa0223130:CDS 45.0%
CCAGAAGAAGAAGGTGAAGA+TGG + chr2_1:72658067-72658086 None:intergenic 45.0%
CCATCTTCACCTTCTTCTTC+TGG - chr2_1:72658064-72658083 Msa0223130:CDS 45.0%
TCTTCTGGCACAAGCTCAAA+TGG - chr2_1:72658079-72658098 Msa0223130:CDS 45.0%
!! GGAATATCTTGTCGGTTCCA+AGG + chr2_1:72658110-72658129 None:intergenic 45.0%
AAACATTCCTGCAGCATGCA+AGG + chr2_1:72658196-72658215 None:intergenic 45.0%
ATGGCCAAGAACATCGTAGA+AGG + chr2_1:72658242-72658261 None:intergenic 45.0%
! CATTTGGTGAAGGCTGGTTA+TGG + chr2_1:72658261-72658280 None:intergenic 45.0%
! TGCTATCATTTGGTGAAGGC+TGG + chr2_1:72658267-72658286 None:intergenic 45.0%
TCTGGGTCAGTGCTATCATT+TGG + chr2_1:72658277-72658296 None:intergenic 45.0%
!! AATGATAGCACTGACCCAGA+TGG - chr2_1:72658277-72658296 Msa0223130:CDS 45.0%
CGCGATCGAAATTTCCATCT+GGG + chr2_1:72658294-72658313 None:intergenic 45.0%
!!! GCTGCTAGTTTTGAGAGAAG+TGG - chr2_1:72658313-72658332 Msa0223130:CDS 45.0%
GAATGACATTCAGCCAGTGA+CGG - chr2_1:72658522-72658541 Msa0223130:CDS 45.0%
AAAACTTCTTCCACCGTCAC+TGG + chr2_1:72658538-72658557 None:intergenic 45.0%
! GCCACCAGAGATTTTAGATG+AGG - chr2_1:72658672-72658691 Msa0223130:CDS 45.0%
ATCTACGACAAACAATGCCG+CGG - chr2_1:72658801-72658820 Msa0223130:CDS 45.0%
! CCTGTAATTCGAAGCTTTGG+AGG + chr2_1:72659231-72659250 None:intergenic 45.0%
CCTCCAAAGCTTCGAATTAC+AGG - chr2_1:72659228-72659247 Msa0223130:CDS 45.0%
CTTTCTGCAGGTCTAAATCC+TGG + chr2_1:72659270-72659289 None:intergenic 45.0%
CAGGATTTAGACCTGCAGAA+AGG - chr2_1:72659268-72659287 Msa0223130:CDS 45.0%
ATTTAGACCTGCAGAAAGGG+TGG - chr2_1:72659272-72659291 Msa0223130:CDS 45.0%
GAACCTTCCTGATGAAACTG+TGG - chr2_1:72659449-72659468 Msa0223130:CDS 45.0%
ATGCCACAGTTTCATCAGGA+AGG + chr2_1:72659455-72659474 None:intergenic 45.0%
TTCTTCCATGGAGTTGCCAA+TGG - chr2_1:72659531-72659550 Msa0223130:CDS 45.0%
AACTTCCATTGGCAACTCCA+TGG + chr2_1:72659539-72659558 None:intergenic 45.0%
!!! GTTTTTCCCGACTCGGTTTA+AGG - chr2_1:72659569-72659588 Msa0223130:CDS 45.0%
AGTGCTTCCTTAAACCGAGT+CGG + chr2_1:72659579-72659598 None:intergenic 45.0%
AGAGATCGATGCTTGAGAGA+GGG - chr2_1:72659658-72659677 Msa0223130:CDS 45.0%
ATGCTTGAGAGAGGGTTGTT+TGG - chr2_1:72659666-72659685 Msa0223130:CDS 45.0%
AGGTCAGGAATAAGAGAGCT+GGG - chr2_1:72659763-72659782 Msa0223130:CDS 45.0%
TGGGTTCAAGCAAATCAGGT+TGG - chr2_1:72659782-72659801 Msa0223130:CDS 45.0%
TAGTCGATGTGGATGGTAAG+TGG - chr2_1:72659865-72659884 Msa0223130:CDS 45.0%
AGTCGATGTGGATGGTAAGT+GGG - chr2_1:72659866-72659885 Msa0223130:CDS 45.0%
!! GATGGTAAGTGGGTTTTGCA+GGG - chr2_1:72659876-72659895 Msa0223130:CDS 45.0%
GAGCTTGTGCCAGAAGAAGA+AGG + chr2_1:72658076-72658095 None:intergenic 50.0%
GCAAAAACCTTGCATGCTGC+AGG - chr2_1:72658186-72658205 Msa0223130:CDS 50.0%
GCGCGATCGAAATTTCCATC+TGG + chr2_1:72658295-72658314 None:intergenic 50.0%
CCTTGTGTCTCGATGACAAC+TGG + chr2_1:72658469-72658488 None:intergenic 50.0%
CCAGTTGTCATCGAGACACA+AGG - chr2_1:72658466-72658485 Msa0223130:CDS 50.0%
! AGGCAACACTCTTCGCCTTT+TGG - chr2_1:72658913-72658932 Msa0223130:CDS 50.0%
! CACTCTTCGCCTTTTGGTGA+TGG - chr2_1:72658919-72658938 Msa0223130:CDS 50.0%
GATAGTCTTCTGACGAGGCA+CGG + chr2_1:72659192-72659211 None:intergenic 50.0%
CTTTACATCCTCGAGGCAGA+TGG + chr2_1:72659382-72659401 None:intergenic 50.0%
CGGTGCGCTATAACTTCCAT+TGG + chr2_1:72659550-72659569 None:intergenic 50.0%
GTGCTTCCTTAAACCGAGTC+GGG + chr2_1:72659578-72659597 None:intergenic 50.0%
GATCTCTGCATGTCTTCACG+AGG + chr2_1:72659645-72659664 None:intergenic 50.0%
CAGAGATCGATGCTTGAGAG+AGG - chr2_1:72659657-72659676 Msa0223130:CDS 50.0%
! TAGCTTGTGAGGGTGCAGAA+AGG - chr2_1:72659709-72659728 Msa0223130:CDS 50.0%
! AGCTTGTGAGGGTGCAGAAA+GGG - chr2_1:72659710-72659729 Msa0223130:CDS 50.0%
AGGGTGCAGAAAGGGTTGAA+AGG - chr2_1:72659718-72659737 Msa0223130:CDS 50.0%
CAGGTCAGGAATAAGAGAGC+TGG - chr2_1:72659762-72659781 Msa0223130:CDS 50.0%
GAGCTGGGTTCAAGCAAATC+AGG - chr2_1:72659778-72659797 Msa0223130:CDS 50.0%
!! GGATGGTAAGTGGGTTTTGC+AGG - chr2_1:72659875-72659894 Msa0223130:CDS 50.0%
!! GTAAGTGGGTTTTGCAGGGA+TGG - chr2_1:72659880-72659899 Msa0223130:CDS 50.0%
! TGGGTTTTGCAGGGATGGAA+GGG - chr2_1:72659885-72659904 Msa0223130:CDS 50.0%
GAGCTTGTGCCAGAAGAAGA+AGG + chr2_1:72658076-72658095 None:intergenic 50.0%
GCAAAAACCTTGCATGCTGC+AGG - chr2_1:72658186-72658205 Msa0223130:CDS 50.0%
GCGCGATCGAAATTTCCATC+TGG + chr2_1:72658295-72658314 None:intergenic 50.0%
CCTTGTGTCTCGATGACAAC+TGG + chr2_1:72658469-72658488 None:intergenic 50.0%
CCAGTTGTCATCGAGACACA+AGG - chr2_1:72658466-72658485 Msa0223130:CDS 50.0%
! AGGCAACACTCTTCGCCTTT+TGG - chr2_1:72658913-72658932 Msa0223130:CDS 50.0%
! CACTCTTCGCCTTTTGGTGA+TGG - chr2_1:72658919-72658938 Msa0223130:CDS 50.0%
GATAGTCTTCTGACGAGGCA+CGG + chr2_1:72659192-72659211 None:intergenic 50.0%
CTTTACATCCTCGAGGCAGA+TGG + chr2_1:72659382-72659401 None:intergenic 50.0%
CGGTGCGCTATAACTTCCAT+TGG + chr2_1:72659550-72659569 None:intergenic 50.0%
GTGCTTCCTTAAACCGAGTC+GGG + chr2_1:72659578-72659597 None:intergenic 50.0%
GATCTCTGCATGTCTTCACG+AGG + chr2_1:72659645-72659664 None:intergenic 50.0%
CAGAGATCGATGCTTGAGAG+AGG - chr2_1:72659657-72659676 Msa0223130:CDS 50.0%
! TAGCTTGTGAGGGTGCAGAA+AGG - chr2_1:72659709-72659728 Msa0223130:CDS 50.0%
! AGCTTGTGAGGGTGCAGAAA+GGG - chr2_1:72659710-72659729 Msa0223130:CDS 50.0%
AGGGTGCAGAAAGGGTTGAA+AGG - chr2_1:72659718-72659737 Msa0223130:CDS 50.0%
CAGGTCAGGAATAAGAGAGC+TGG - chr2_1:72659762-72659781 Msa0223130:CDS 50.0%
GAGCTGGGTTCAAGCAAATC+AGG - chr2_1:72659778-72659797 Msa0223130:CDS 50.0%
!! GGATGGTAAGTGGGTTTTGC+AGG - chr2_1:72659875-72659894 Msa0223130:CDS 50.0%
!! GTAAGTGGGTTTTGCAGGGA+TGG - chr2_1:72659880-72659899 Msa0223130:CDS 50.0%
! TGGGTTTTGCAGGGATGGAA+GGG - chr2_1:72659885-72659904 Msa0223130:CDS 50.0%
TGACATTCAGCCAGTGACGG+TGG - chr2_1:72658525-72658544 Msa0223130:CDS 55.0%
CTGTGGCGAGCTACGATCAA+AGG - chr2_1:72658863-72658882 Msa0223130:CDS 55.0%
CGCTGGAGTCCATCACCAAA+AGG + chr2_1:72658931-72658950 None:intergenic 55.0%
CCAGCAGTTGCCACATCAAG+AGG + chr2_1:72659018-72659037 None:intergenic 55.0%
CCTCTTGATGTGGCAACTGC+TGG - chr2_1:72659015-72659034 Msa0223130:CDS 55.0%
CACTCTCTGCAAAGGAGACG+CGG + chr2_1:72659073-72659092 None:intergenic 55.0%
TAGACCTGCAGAAAGGGTGG+AGG - chr2_1:72659275-72659294 Msa0223130:CDS 55.0%
GTTTCCTCCACCCTTTCTGC+AGG + chr2_1:72659282-72659301 None:intergenic 55.0%
GCAGAAAGGGTGGAGGAAAC+CGG - chr2_1:72659282-72659301 Msa0223130:CDS 55.0%
AAAGGGTGGAGGAAACCGGA+CGG - chr2_1:72659286-72659305 Msa0223130:CDS 55.0%
!! GTAGTTTGCTAGACGCCGTC+CGG + chr2_1:72659304-72659323 None:intergenic 55.0%
ATGGGAAACCATCTGCCTCG+AGG - chr2_1:72659371-72659390 Msa0223130:CDS 55.0%
TGCCACTGCTTGTAGGTCTC+AGG + chr2_1:72659744-72659763 None:intergenic 55.0%
TGAGACCTACAAGCAGTGGC+AGG - chr2_1:72659743-72659762 Msa0223130:CDS 55.0%
! GTGGGTTTTGCAGGGATGGA+AGG - chr2_1:72659884-72659903 Msa0223130:CDS 55.0%
TGACATTCAGCCAGTGACGG+TGG - chr2_1:72658525-72658544 Msa0223130:CDS 55.0%
CTGTGGCGAGCTACGATCAA+AGG - chr2_1:72658863-72658882 Msa0223130:CDS 55.0%
CGCTGGAGTCCATCACCAAA+AGG + chr2_1:72658931-72658950 None:intergenic 55.0%
CCAGCAGTTGCCACATCAAG+AGG + chr2_1:72659018-72659037 None:intergenic 55.0%
CCTCTTGATGTGGCAACTGC+TGG - chr2_1:72659015-72659034 Msa0223130:CDS 55.0%
CACTCTCTGCAAAGGAGACG+CGG + chr2_1:72659073-72659092 None:intergenic 55.0%
TAGACCTGCAGAAAGGGTGG+AGG - chr2_1:72659275-72659294 Msa0223130:CDS 55.0%
GTTTCCTCCACCCTTTCTGC+AGG + chr2_1:72659282-72659301 None:intergenic 55.0%
GCAGAAAGGGTGGAGGAAAC+CGG - chr2_1:72659282-72659301 Msa0223130:CDS 55.0%
AAAGGGTGGAGGAAACCGGA+CGG - chr2_1:72659286-72659305 Msa0223130:CDS 55.0%
!! GTAGTTTGCTAGACGCCGTC+CGG + chr2_1:72659304-72659323 None:intergenic 55.0%
ATGGGAAACCATCTGCCTCG+AGG - chr2_1:72659371-72659390 Msa0223130:CDS 55.0%
TGCCACTGCTTGTAGGTCTC+AGG + chr2_1:72659744-72659763 None:intergenic 55.0%
TGAGACCTACAAGCAGTGGC+AGG - chr2_1:72659743-72659762 Msa0223130:CDS 55.0%
! GTGGGTTTTGCAGGGATGGA+AGG - chr2_1:72659884-72659903 Msa0223130:CDS 55.0%
GACAAACAATGCCGCGGCAG+TGG - chr2_1:72658807-72658826 Msa0223130:CDS 60.0%
! ATGCCGCGGCAGTGGATTTG+TGG - chr2_1:72658815-72658834 Msa0223130:CDS 60.0%
GGTCCACAAATCCACTGCCG+CGG + chr2_1:72658821-72658840 None:intergenic 60.0%
!! CGAAGCTTTGGAGGACCACC+AGG + chr2_1:72659222-72659241 None:intergenic 60.0%
GGAATTGAGCTTCCTCGGCC+AGG - chr2_1:72659249-72659268 Msa0223130:CDS 60.0%
GCAGGTCTAAATCCTGGCCG+AGG + chr2_1:72659264-72659283 None:intergenic 60.0%
!! GCGCACCGTTTTTCCCGACT+CGG - chr2_1:72659562-72659581 Msa0223130:CDS 60.0%
GGCCTGAGACCTACAAGCAG+TGG - chr2_1:72659739-72659758 Msa0223130:CDS 60.0%
CCTGACCTGCCACTGCTTGT+AGG + chr2_1:72659751-72659770 None:intergenic 60.0%
CCTACAAGCAGTGGCAGGTC+AGG - chr2_1:72659748-72659767 Msa0223130:CDS 60.0%
GACAAACAATGCCGCGGCAG+TGG - chr2_1:72658807-72658826 Msa0223130:CDS 60.0%
! ATGCCGCGGCAGTGGATTTG+TGG - chr2_1:72658815-72658834 Msa0223130:CDS 60.0%
GGTCCACAAATCCACTGCCG+CGG + chr2_1:72658821-72658840 None:intergenic 60.0%
!! CGAAGCTTTGGAGGACCACC+AGG + chr2_1:72659222-72659241 None:intergenic 60.0%
GGAATTGAGCTTCCTCGGCC+AGG - chr2_1:72659249-72659268 Msa0223130:CDS 60.0%
GCAGGTCTAAATCCTGGCCG+AGG + chr2_1:72659264-72659283 None:intergenic 60.0%
!! GCGCACCGTTTTTCCCGACT+CGG - chr2_1:72659562-72659581 Msa0223130:CDS 60.0%
GGCCTGAGACCTACAAGCAG+TGG - chr2_1:72659739-72659758 Msa0223130:CDS 60.0%
CCTGACCTGCCACTGCTTGT+AGG + chr2_1:72659751-72659770 None:intergenic 60.0%
CCTACAAGCAGTGGCAGGTC+AGG - chr2_1:72659748-72659767 Msa0223130:CDS 60.0%
TTCTGACGAGGCACGGCCAC+TGG + chr2_1:72659185-72659204 None:intergenic 65.0%
TTCTGACGAGGCACGGCCAC+TGG + chr2_1:72659185-72659204 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 72657977 72659947 72657977 ID=Msa0223130;Name=Msa0223130
chr2_1 mRNA 72657977 72659947 72657977 ID=Msa0223130-mRNA-1;Parent=Msa0223130;Name=Msa0223130-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|656
chr2_1 exon 72657977 72659947 72657977 ID=Msa0223130-mRNA-1:exon:21105;Parent=Msa0223130-mRNA-1
chr2_1 CDS 72657977 72659947 72657977 ID=Msa0223130-mRNA-1:cds;Parent=Msa0223130-mRNA-1
Gene Sequence

>Msa0223130

ATGCTTTCTCCAAACACTTTTCTGCAAGATTTTAGTGTTCCAAACTCAGTAAACCAAGTAACAAATGGGTTTGAATTAGAAGATTTACCATCTTCACCTTCTTCTTCTGGCACAAGCTCAAATGGACAATCCTTGGAACCGACAAGATATTCCAATAACATCCTAAGATACATAAATGACATCCTCATGGATGAAGAAGATGACTTGGAGCAAAAACCTTGCATGCTGCAGGAATGTTTAAGACTTCAGGCCGCTGAAAAGTCCTTCTACGATGTTCTTGGCCATAACCAGCCTTCACCAAATGATAGCACTGACCCAGATGGAAATTTCGATCGCGCTGCTAGTTTTGAGAGAAGTGGCAGCAGCTGCATCGCTGATAACTCATATGAATCTAACTTGGTCAACCATGTCGTCGAATTCGAATCTTCTTCCATACAACTACAAAGTCCTTTAATAGAAAAAAACTACTATGACTTGACAGAAGCTGATCCAGTTGTCATCGAGACACAAGGTGCTAACCATTTTCACAATGGAACTTGGAGTTGGAATGACATTCAGCCAGTGACGGTGGAAGAAGTTTTACCTTCAACAGAACCAGAAAAAAGAAGTCGTAAGATGGATAGTGATAATGATACTTGTAATGAACAAGAGGGAAGAGGTAGCAAAGTTTCAGCTATTTTCTCTGATGAATTAGAGCCACCAGAGATTTTAGATGAGGTACTGCTTTATCAGAAAGGAAAGACTCTCACTCAACACCAACAAGCATCACATAATGTTGATTCAGGAGGAAAGGCAAAAGAAACACATTCGCGTTTGAAGAAAGCATCTACGACAAACAATGCCGCGGCAGTGGATTTGTGGACCATGCTAAATCAATGTGCACAAGCTGTGGCGAGCTACGATCAAAGGAATACAGATGAGCTACTCAAACAGATTAGGCAACACTCTTCGCCTTTTGGTGATGGACTCCAGCGTTTAGCTCATTACTTTGCTAATGGTCTTGAGATAAGATTGGCTGCTGAGACACCATCATATCAGCCTCTTGATGTGGCAACTGCTGGAGATATGTTGAAAGCTTACAAACTATTTGTTACCGCGTCTCCTTTGCAGAGAGTGACAAATACTTTGCTAACCAAAACGATTTTCAAAATAGTGAAGAACGAGAGCAGCGTTCATGTTATTGATTTTGGAATTTTCTATGGTTTCCAGTGGCCGTGCCTCGTCAGAAGACTATCATTAAGACCTGGTGGTCCTCCAAAGCTTCGAATTACAGGAATTGAGCTTCCTCGGCCAGGATTTAGACCTGCAGAAAGGGTGGAGGAAACCGGACGGCGTCTAGCAAACTACTGCAAGAAGTTCAATGTACCATTTGAATACAACTTCATTGCACAGAAATGGGAAACCATCTGCCTCGAGGATGTAAAGATAGACAGGAATGAAATAACTTTTGTTAGCTGTTTGTACAGACTGAAGAACCTTCCTGATGAAACTGTGGCATTGAATTGTCCAAGAGAAGATGTGTTGAAGCTGATCAGGAAGATTAATCCAAAAGTCTTCTTCCATGGAGTTGCCAATGGAAGTTATAGCGCACCGTTTTTCCCGACTCGGTTTAAGGAAGCACTCTACCACTTCTCATCGTTGTTTGATATGTTTGAGGCTAACGTGCCTCGTGAAGACATGCAGAGATCGATGCTTGAGAGAGGGTTGTTTGGAAGAGATGCCATAAATGTTATAGCTTGTGAGGGTGCAGAAAGGGTTGAAAGGCCTGAGACCTACAAGCAGTGGCAGGTCAGGAATAAGAGAGCTGGGTTCAAGCAAATCAGGTTGGATTCTGATTTAGTGAATGAAACGAAAACAATCGTGAAGAAGGAATACCATAAAGATTTTGTAGTCGATGTGGATGGTAAGTGGGTTTTGCAGGGATGGAAGGGACGCATTTTGAATGCTTTATCAGCTTGGGTTCCTGCTTAA
Protein sequence

>Msa0223130

MLSPNTFLQDFSVPNSVNQVTNGFELEDLPSSPSSSGTSSNGQSLEPTRYSNNILRYINDILMDEEDDLEQKPCMLQECLRLQAAEKSFYDVLGHNQPSPNDSTDPDGNFDRAASFERSGSSCIADNSYESNLVNHVVEFESSSIQLQSPLIEKNYYDLTEADPVVIETQGANHFHNGTWSWNDIQPVTVEEVLPSTEPEKRSRKMDSDNDTCNEQEGRGSKVSAIFSDELEPPEILDEVLLYQKGKTLTQHQQASHNVDSGGKAKETHSRLKKASTTNNAAAVDLWTMLNQCAQAVASYDQRNTDELLKQIRQHSSPFGDGLQRLAHYFANGLEIRLAAETPSYQPLDVATAGDMLKAYKLFVTASPLQRVTNTLLTKTIFKIVKNESSVHVIDFGIFYGFQWPCLVRRLSLRPGGPPKLRITGIELPRPGFRPAERVEETGRRLANYCKKFNVPFEYNFIAQKWETICLEDVKIDRNEITFVSCLYRLKNLPDETVALNCPREDVLKLIRKINPKVFFHGVANGSYSAPFFPTRFKEALYHFSSLFDMFEANVPREDMQRSMLERGLFGRDAINVIACEGAERVERPETYKQWQVRNKRAGFKQIRLDSDLVNETKTIVKKEYHKDFVVDVDGKWVLQGWKGRILNALSAWVPA*