Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0304910 | Msa0341300 | 0.834157 | 3.525230e-56 | -8.615850e-47 |
| Msa0341300 | Msa0341310 | 0.835918 | 1.268210e-56 | -8.615850e-47 |
| Msa0341300 | Msa1417520 | 0.831185 | 1.926886e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTGGTATTGATAGGTTTA+TGG | 0.282840 | 2_4:-62782200 | Msa0341300:CDS |
| GGAGTCTATTGATAATAAAT+AGG | 0.334817 | 2_4:-62782179 | Msa0341300:CDS |
| TTACCATGTACAGGGCAAAT+TGG | 0.376909 | 2_4:-62782096 | Msa0341300:CDS |
| ATATCCCGAGCGTAAGAGTT+TGG | 0.396341 | 2_4:+62782149 | None:intergenic |
| TTCAGGCAGGTGGTATTGAT+AGG | 0.412603 | 2_4:-62782207 | Msa0341300:CDS |
| GGAGCAGAATTACCATGTAC+AGG | 0.455678 | 2_4:-62782105 | Msa0341300:CDS |
| ACTCTTACGCTCGGGATATG+TGG | 0.479887 | 2_4:-62782145 | Msa0341300:CDS |
| TCTATTGATAATAAATAGGA+AGG | 0.488441 | 2_4:-62782175 | Msa0341300:CDS |
| TGGTAATTCTGCTCCTAAAA+CGG | 0.492433 | 2_4:+62782113 | None:intergenic |
| CCTCCAATTTGCCCTGTACA+TGG | 0.502924 | 2_4:+62782093 | None:intergenic |
| ACAGGGCAAATTGGAGGGAT+AGG | 0.503759 | 2_4:-62782087 | Msa0341300:CDS |
| CAGGGCAAATTGGAGGGATA+GGG | 0.535026 | 2_4:-62782086 | Msa0341300:CDS |
| CCATGTACAGGGCAAATTGG+AGG | 0.546864 | 2_4:-62782093 | Msa0341300:CDS |
| GGGGCCAAACTCTTACGCTC+GGG | 0.570745 | 2_4:-62782153 | Msa0341300:CDS |
| TATTGATAATAAATAGGAAG+GGG | 0.576175 | 2_4:-62782173 | Msa0341300:CDS |
| CATGTACAGGGCAAATTGGA+GGG | 0.580326 | 2_4:-62782092 | Msa0341300:CDS |
| CTATTGATAATAAATAGGAA+GGG | 0.613535 | 2_4:-62782174 | Msa0341300:CDS |
| GAGCAGAATTACCATGTACA+GGG | 0.654144 | 2_4:-62782104 | Msa0341300:CDS |
| ATTGATAATAAATAGGAAGG+GGG | 0.655796 | 2_4:-62782172 | Msa0341300:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTATTGATAATAAATAGGA+AGG | - | chr2_4:62782094-62782113 | Msa0341300:CDS | 20.0% |
| !!! | CTATTGATAATAAATAGGAA+GGG | - | chr2_4:62782095-62782114 | Msa0341300:CDS | 20.0% |
| !!! | TATTGATAATAAATAGGAAG+GGG | - | chr2_4:62782096-62782115 | Msa0341300:CDS | 20.0% |
| !! | GGAGTCTATTGATAATAAAT+AGG | - | chr2_4:62782090-62782109 | Msa0341300:CDS | 25.0% |
| !! | ATTGATAATAAATAGGAAGG+GGG | - | chr2_4:62782097-62782116 | Msa0341300:CDS | 25.0% |
| !! | GTTCTCAACTTCTCATTTTT+GGG | - | chr2_4:62782205-62782224 | Msa0341300:CDS | 30.0% |
| !! | AGGTGGTATTGATAGGTTTA+TGG | - | chr2_4:62782069-62782088 | Msa0341300:CDS | 35.0% |
| TGGTAATTCTGCTCCTAAAA+CGG | + | chr2_4:62782159-62782178 | None:intergenic | 35.0% | |
| ! | GGTTCTCAACTTCTCATTTT+TGG | - | chr2_4:62782204-62782223 | Msa0341300:CDS | 35.0% |
| ! | GTGGATCAATACTCCGTTTT+AGG | - | chr2_4:62782143-62782162 | Msa0341300:CDS | 40.0% |
| GAGCAGAATTACCATGTACA+GGG | - | chr2_4:62782165-62782184 | Msa0341300:CDS | 40.0% | |
| TTACCATGTACAGGGCAAAT+TGG | - | chr2_4:62782173-62782192 | Msa0341300:CDS | 40.0% | |
| !! | TTCAGGCAGGTGGTATTGAT+AGG | - | chr2_4:62782062-62782081 | Msa0341300:CDS | 45.0% |
| ATATCCCGAGCGTAAGAGTT+TGG | + | chr2_4:62782123-62782142 | None:intergenic | 45.0% | |
| GGAGCAGAATTACCATGTAC+AGG | - | chr2_4:62782164-62782183 | Msa0341300:CDS | 45.0% | |
| CATGTACAGGGCAAATTGGA+GGG | - | chr2_4:62782177-62782196 | Msa0341300:CDS | 45.0% | |
| ACTCTTACGCTCGGGATATG+TGG | - | chr2_4:62782124-62782143 | Msa0341300:CDS | 50.0% | |
| CCTCCAATTTGCCCTGTACA+TGG | + | chr2_4:62782179-62782198 | None:intergenic | 50.0% | |
| CCATGTACAGGGCAAATTGG+AGG | - | chr2_4:62782176-62782195 | Msa0341300:CDS | 50.0% | |
| ACAGGGCAAATTGGAGGGAT+AGG | - | chr2_4:62782182-62782201 | Msa0341300:CDS | 50.0% | |
| CAGGGCAAATTGGAGGGATA+GGG | - | chr2_4:62782183-62782202 | Msa0341300:CDS | 50.0% | |
| GGGGGCCAAACTCTTACGCT+CGG | - | chr2_4:62782115-62782134 | Msa0341300:CDS | 60.0% | |
| GGGGCCAAACTCTTACGCTC+GGG | - | chr2_4:62782116-62782135 | Msa0341300:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 62782056 | 62782235 | 62782056 | ID=Msa0341300;Name=Msa0341300 |
| chr2_4 | mRNA | 62782056 | 62782235 | 62782056 | ID=Msa0341300-mRNA-1;Parent=Msa0341300;Name=Msa0341300-mRNA-1;_AED=0.93;_eAED=1.00;_QI=0|-1|0|1|-1|1|1|0|59 |
| chr2_4 | exon | 62782056 | 62782235 | 62782056 | ID=Msa0341300-mRNA-1:exon:20037;Parent=Msa0341300-mRNA-1 |
| chr2_4 | CDS | 62782056 | 62782235 | 62782056 | ID=Msa0341300-mRNA-1:cds;Parent=Msa0341300-mRNA-1 |
| Gene Sequence |
| Protein sequence |
