AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0342460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0342460 MtrunA17_Chr5g0416291 79.856 139 27 1 1 139 1 138 1.80e-80 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 55 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAACTCTGCCAAGAAAA+TGG 0.261876 3_1:-148514 Msa0342460:three_prime_UTR
CTTCCTTGTGAACGGTATTC+AGG 0.279428 3_1:+148860 None:intergenic
TGATGTAAGCTAGTTGAATT+TGG 0.299146 3_1:-148670 Msa0342460:three_prime_UTR
CCTTTGTTTACCGATTAAAT+AGG 0.316807 3_1:-152288 Msa0342460:five_prime_UTR
TGCTTCATTGCCTATTTAAT+CGG 0.321088 3_1:+152278 None:intergenic
AATTAAAGTGCCACGGAAAC+AGG 0.331199 3_1:+148584 None:intergenic
ATACAACATACATCGGAATT+CGG 0.360569 3_1:+151716 None:intergenic
GGGCATGGTGATTGGAAATC+TGG 0.361101 3_1:-151768 Msa0342460:CDS
TTAGTGAGTAATATTGCTTT+AGG 0.370896 3_1:-148491 Msa0342460:three_prime_UTR
AATGTCAAGGGCATGGTGAT+TGG 0.388172 3_1:-151776 Msa0342460:CDS
TACAACATACATCGGAATTC+GGG 0.397694 3_1:+151717 None:intergenic
ACTTATCATTCAACGGTAGA+AGG 0.401385 3_1:+151975 None:intergenic
ACACATTGATACAGCTGCTT+AGG 0.407272 3_1:+152248 None:intergenic
AGAATAACATTGAAATATCT+TGG 0.412668 3_1:-148618 Msa0342460:three_prime_UTR
TTGAGCGCCATAACTCTTCC+AGG 0.436383 3_1:+152212 None:intergenic
TGCAATAACAGCATAACTCT+TGG 0.440937 3_1:+151943 None:intergenic
TGGAGTTGCCATGACAGATC+TGG 0.447860 3_1:-152171 Msa0342460:CDS
ATGACAGATCTGGAGAATAA+AGG 0.450592 3_1:-152161 Msa0342460:CDS
GACGACTATCGAGCTCTTCA+AGG 0.458353 3_1:+151744 None:intergenic
TCACTTATTAGTTCCAGAAA+AGG 0.459683 3_1:-148774 Msa0342460:three_prime_UTR
CTACCGTTGAATGATAAGTC+TGG 0.463316 3_1:-151971 Msa0342460:CDS
AACGTTGGCACTGAGTATCT+TGG 0.463373 3_1:-152191 Msa0342460:CDS
AACTTACCTCGAGAACTAGC+TGG 0.476288 3_1:+151533 None:intergenic
CACATTGATACAGCTGCTTA+GGG 0.483630 3_1:+152249 None:intergenic
TGCATCTGAAATCAAGGAGT+TGG 0.485121 3_1:-151924 Msa0342460:intron
TTAATTAATTATAGATTGAT+CGG 0.485918 3_1:-151802 Msa0342460:intron
ATAAAGGCCTGGAGGACTCT+AGG 0.499130 3_1:-152145 Msa0342460:CDS
AGCAATACCTGGAAGAGTTA+TGG 0.502145 3_1:-152219 Msa0342460:CDS
ACCTACTCCTAGAGTCCTCC+AGG 0.502982 3_1:+152138 None:intergenic
AGATCTGGAGAATAAAGGCC+TGG 0.509221 3_1:-152156 Msa0342460:CDS
GTTGAATTTGGTCACAATTG+TGG 0.509775 3_1:-148658 Msa0342460:three_prime_UTR
GCAATAACAGCATAACTCTT+GGG 0.515127 3_1:+151944 None:intergenic
TCAACTAGCTTACATCAAGA+AGG 0.518691 3_1:+148676 None:intergenic
TGTTATTGCATCTGAAATCA+AGG 0.519499 3_1:-151930 Msa0342460:CDS
TCGAGAACTAGCTGGGCTCT+CGG 0.532019 3_1:+151541 None:intergenic
TGTGTATGCAAAGCAATACC+TGG 0.552102 3_1:-152230 Msa0342460:CDS
TTTATTCTCCAGATCTGTCA+TGG 0.562890 3_1:+152163 None:intergenic
CCTATTTAATCGGTAAACAA+AGG 0.572550 3_1:+152288 None:intergenic
CGCGGTACATCCTGTTTCCG+TGG 0.576680 3_1:-148594 Msa0342460:three_prime_UTR
ACTTACCTCGAGAACTAGCT+GGG 0.582728 3_1:+151534 None:intergenic
AAGAATCAATTAAAGTGCCA+CGG 0.600117 3_1:+148577 None:intergenic
TTTCCAGACTTATCATTCAA+CGG 0.609359 3_1:+151968 None:intergenic
GATTGATCGGAACAATGTCA+AGG 0.610220 3_1:-151789 Msa0342460:CDS
AGAGTTATGGCGCTCAACGT+TGG 0.612283 3_1:-152206 Msa0342460:CDS
AGTCTAGCTCATAGCACCAC+CGG 0.613408 3_1:-148839 Msa0342460:exon
TCGGAACAATGTCAAGGGCA+TGG 0.615844 3_1:-151783 Msa0342460:CDS
GAGCTAGACTTCCTTGTGAA+CGG 0.617106 3_1:+148852 None:intergenic
CATACATCGGAATTCGGGAG+AGG 0.623357 3_1:+151722 None:intergenic
ATTGATCGGAACAATGTCAA+GGG 0.639957 3_1:-151788 Msa0342460:CDS
ACATTGAAATATCTTGGTCG+CGG 0.652374 3_1:-148612 Msa0342460:three_prime_UTR
GCCTGGAGGACTCTAGGAGT+AGG 0.657769 3_1:-152139 Msa0342460:intron
AGAACTAGCTGGGCTCTCGG+TGG 0.660515 3_1:+151544 None:intergenic
GTTCCTGAATACCGTTCACA+AGG 0.661660 3_1:-148863 Msa0342460:CDS
GAGAGCCCAGCTAGTTCTCG+AGG 0.662115 3_1:-151539 Msa0342460:intron
TCTGGAGAATAAAGGCCTGG+AGG 0.698062 3_1:-152153 Msa0342460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAATTAAAATAATA+CGG - chr3_1:149981-150000 Msa0342460:intron 0.0%
!! AATAAAAAACTAAAACAAAA+GGG + chr3_1:148657-148676 None:intergenic 10.0%
!! AAATAAAAAACTAAAACAAA+AGG + chr3_1:148658-148677 None:intergenic 10.0%
!! AAAAAAAAAAAAACATTAAC+GGG - chr3_1:148738-148757 Msa0342460:three_prime_UTR 10.0%
!! TTAATTAATTATAGATTGAT+CGG - chr3_1:148952-148971 Msa0342460:intron 10.0%
!! ATAAAAAACTAAAACAAAAG+GGG + chr3_1:148656-148675 None:intergenic 15.0%
!!! ATTTTTTATTCAGATATGAA+GGG + chr3_1:149971-149990 None:intergenic 15.0%
!!! AATTTTTTATTCAGATATGA+AGG + chr3_1:149972-149991 None:intergenic 15.0%
!!! AGAAAAAACCAATTTTATTT+AGG + chr3_1:150495-150514 None:intergenic 15.0%
!! TAAATAAAGGTTAAATACTA+TGG - chr3_1:150730-150749 Msa0342460:intron 15.0%
!! ATAAATTAATGTATATGTAG+CGG - chr3_1:150843-150862 Msa0342460:intron 15.0%
!! CTTATAAGCTATAAAAAAAA+TGG - chr3_1:151622-151641 Msa0342460:CDS 15.0%
!! ATATAGTAAAATAACATTGT+AGG - chr3_1:151649-151668 Msa0342460:intron 15.0%
!!! ATTAATATTGACATTTTGTC+CGG - chr3_1:148909-148928 Msa0342460:intron 20.0%
!! ATGAAAATGATTATAAGTAC+CGG + chr3_1:148931-148950 None:intergenic 20.0%
!! ATTAAACATACAACATACAT+CGG + chr3_1:149048-149067 None:intergenic 20.0%
!!! TTTTCTTAATCCTTTATGTT+TGG - chr3_1:149279-149298 Msa0342460:intron 20.0%
!!! AAAGAAAATTGATTGATAGT+TGG - chr3_1:149788-149807 Msa0342460:intron 20.0%
!!! TTTTTTATTCAGATATGAAG+GGG + chr3_1:149970-149989 None:intergenic 20.0%
!! AAAATTAAAATAATACGGGT+CGG - chr3_1:149986-150005 Msa0342460:intron 20.0%
!!! GAACATTTTTGGTTTAATTT+GGG + chr3_1:150062-150081 None:intergenic 20.0%
!!! TGAACATTTTTGGTTTAATT+TGG + chr3_1:150063-150082 None:intergenic 20.0%
!!! TGTTTGTCATTTTTTCTATT+CGG - chr3_1:150108-150127 Msa0342460:intron 20.0%
!!! TTTTTCTATTCGGTTTTTTT+CGG - chr3_1:150118-150137 Msa0342460:intron 20.0%
!!! AAATCAGAAAGCATTTTATT+AGG + chr3_1:150289-150308 None:intergenic 20.0%
!! TTATAATAAACTATGAAGCA+TGG - chr3_1:150369-150388 Msa0342460:intron 20.0%
!!! AATTGGTTTTTTCTTTACTT+CGG - chr3_1:150501-150520 Msa0342460:intron 20.0%
!! TGATAAATGTTGATCAATTA+CGG - chr3_1:150651-150670 Msa0342460:intron 20.0%
!!! AAAGTTGATTACAATGTTTT+TGG - chr3_1:150687-150706 Msa0342460:intron 20.0%
!! GATAGTAGATGAATAAATAA+AGG - chr3_1:150717-150736 Msa0342460:intron 20.0%
!! GCAATTTATTTCATTGAAAA+AGG + chr3_1:150771-150790 None:intergenic 20.0%
!!! TCTAGCATTTTTTGAAATTT+CGG + chr3_1:151084-151103 None:intergenic 20.0%
!! TTAATATTTACTACATTCCT+AGG - chr3_1:151412-151431 Msa0342460:intron 20.0%
!! AATATAGAAGTTACAAAGAA+GGG + chr3_1:151532-151551 None:intergenic 20.0%
!! TTTGTAACTTCTATATTCTT+GGG - chr3_1:151535-151554 Msa0342460:intron 20.0%
!! ATAACATTGTAGGTTAAAAT+TGG - chr3_1:151659-151678 Msa0342460:intron 20.0%
!!! ATTCAAACTAATAACTTTTC+CGG + chr3_1:151937-151956 None:intergenic 20.0%
!! AGAATAACATTGAAATATCT+TGG - chr3_1:152136-152155 Msa0342460:intron 20.0%
!!! GGTGGAGTTTTATTTATTTT+GGG - chr3_1:149499-149518 Msa0342460:intron 25.0%
! CTGCTTCAACAAAAAAAAAT+GGG - chr3_1:149612-149631 Msa0342460:intron 25.0%
! GAATAGAAAAAATGACAAAC+AGG + chr3_1:150109-150128 None:intergenic 25.0%
!!! TTTTCTATTCGGTTTTTTTC+GGG - chr3_1:150119-150138 Msa0342460:intron 25.0%
! AAATTTGAACAGAATCTCTA+GGG + chr3_1:150174-150193 None:intergenic 25.0%
! AAAATTTGAACAGAATCTCT+AGG + chr3_1:150175-150194 None:intergenic 25.0%
! GCTTCATAGTTTATTATAAG+TGG + chr3_1:150368-150387 None:intergenic 25.0%
! AATGGTGTCCTAAATAAAAT+TGG - chr3_1:150484-150503 Msa0342460:intron 25.0%
!! AAACATCTTTATTTCTCTCT+TGG - chr3_1:150592-150611 Msa0342460:intron 25.0%
! GATAAATGTTGATCAATTAC+GGG - chr3_1:150652-150671 Msa0342460:intron 25.0%
! ATAAATGTTGATCAATTACG+GGG - chr3_1:150653-150672 Msa0342460:intron 25.0%
! ACGGCTAATGTAAAAAATAT+AGG + chr3_1:150884-150903 None:intergenic 25.0%
! TTGTTGAAATAAGAGGTAAA+GGG + chr3_1:151165-151184 None:intergenic 25.0%
! ATTGTTGAAATAAGAGGTAA+AGG + chr3_1:151166-151185 None:intergenic 25.0%
! CTAGGATTATTTGTAAACAT+GGG - chr3_1:151430-151449 Msa0342460:intron 25.0%
! TTATTTGTAAACATGGGAAA+TGG - chr3_1:151436-151455 Msa0342460:intron 25.0%
! TATTTGTAAACATGGGAAAT+GGG - chr3_1:151437-151456 Msa0342460:intron 25.0%
! AAAGTTAGGAAATTAATTCC+TGG + chr3_1:151493-151512 None:intergenic 25.0%
! GAATATAGAAGTTACAAAGA+AGG + chr3_1:151533-151552 None:intergenic 25.0%
! CTTTGTAACTTCTATATTCT+TGG - chr3_1:151534-151553 Msa0342460:intron 25.0%
! AAAAATGTCGTGTTTAGTAA+GGG + chr3_1:151591-151610 None:intergenic 25.0%
! TAAAAATGTCGTGTTTAGTA+AGG + chr3_1:151592-151611 None:intergenic 25.0%
! AAAATGATTCCTTTCTTTGT+TGG - chr3_1:151739-151758 Msa0342460:CDS 25.0%
! AAACAAAGAAGAATCAACAA+AGG + chr3_1:151807-151826 None:intergenic 25.0%
! TTAGTGAGTAATATTGCTTT+AGG - chr3_1:152263-152282 Msa0342460:CDS 25.0%
CCTATTTAATCGGTAAACAA+AGG + chr3_1:148469-148488 None:intergenic 30.0%
! CCTTTGTTTACCGATTAAAT+AGG - chr3_1:148466-148485 Msa0342460:three_prime_UTR 30.0%
TGCTTCATTGCCTATTTAAT+CGG + chr3_1:148479-148498 None:intergenic 30.0%
TTTCCAGACTTATCATTCAA+CGG + chr3_1:148789-148808 None:intergenic 30.0%
! TGTTATTGCATCTGAAATCA+AGG - chr3_1:148824-148843 Msa0342460:three_prime_UTR 30.0%
ATACAACATACATCGGAATT+CGG + chr3_1:149041-149060 None:intergenic 30.0%
!! TATTTGTTTTCGCAAGCTTT+TGG + chr3_1:149161-149180 None:intergenic 30.0%
! TTAAGAAAACACATGATGGA+CGG + chr3_1:149268-149287 None:intergenic 30.0%
AGGATTAAGAAAACACATGA+TGG + chr3_1:149272-149291 None:intergenic 30.0%
TAATCCTTTATGTTTGGTCT+TGG - chr3_1:149285-149304 Msa0342460:intron 30.0%
! AATCCTTTATGTTTGGTCTT+GGG - chr3_1:149286-149305 Msa0342460:intron 30.0%
!! GTTATATGTTTTGAGTGTTG+TGG - chr3_1:149352-149371 Msa0342460:intron 30.0%
! TTGAGTGTTGTGGAAATTTA+AGG - chr3_1:149362-149381 Msa0342460:intron 30.0%
!!! GGGTGGAGTTTTATTTATTT+TGG - chr3_1:149498-149517 Msa0342460:intron 30.0%
GCTGCTTCAACAAAAAAAAA+TGG - chr3_1:149611-149630 Msa0342460:intron 30.0%
ACAATGATTTACACTTTGCT+AGG - chr3_1:149726-149745 Msa0342460:intron 30.0%
CAATGATTTACACTTTGCTA+GGG - chr3_1:149727-149746 Msa0342460:intron 30.0%
! AATTGATTGATAGTTGGTAC+TGG - chr3_1:149794-149813 Msa0342460:intron 30.0%
TAGAAAAAATGACAAACAGG+AGG + chr3_1:150106-150125 None:intergenic 30.0%
AATTTGAACAGAATCTCTAG+GGG + chr3_1:150173-150192 None:intergenic 30.0%
TTCCTTCATTGTACAAAATC+TGG - chr3_1:150193-150212 Msa0342460:intron 30.0%
TAATGTATATGTAGCGGTAT+CGG - chr3_1:150849-150868 Msa0342460:intron 30.0%
! GAACTCTTTTGTGATTTATG+AGG - chr3_1:150952-150971 Msa0342460:intron 30.0%
!!! GCATTTTTTGAAATTTCGGA+AGG + chr3_1:151080-151099 None:intergenic 30.0%
TGTTGAAATAAGAGGTAAAG+GGG + chr3_1:151164-151183 None:intergenic 30.0%
! ACCTCTTATTTCAACAATCT+TGG - chr3_1:151168-151187 Msa0342460:intron 30.0%
CTCGTGATGTAAAACAATTA+GGG - chr3_1:151288-151307 Msa0342460:intron 30.0%
TCGTGATGTAAAACAATTAG+GGG - chr3_1:151289-151308 Msa0342460:intron 30.0%
GATTTCACTTCTTAATTCCT+GGG + chr3_1:151358-151377 None:intergenic 30.0%
TGATTTCACTTCTTAATTCC+TGG + chr3_1:151359-151378 None:intergenic 30.0%
AAGAAGTGAAATCATAATCC+CGG - chr3_1:151365-151384 Msa0342460:intron 30.0%
!!! TACTTTTCAAAAGAAGCGTT+TGG - chr3_1:151389-151408 Msa0342460:intron 30.0%
CCATGTTTACAAATAATCCT+AGG + chr3_1:151432-151451 None:intergenic 30.0%
CCTAGGATTATTTGTAAACA+TGG - chr3_1:151429-151448 Msa0342460:intron 30.0%
! CCTATGTTTGTTACAAAGTT+AGG + chr3_1:151507-151526 None:intergenic 30.0%
CCTAACTTTGTAACAAACAT+AGG - chr3_1:151504-151523 Msa0342460:intron 30.0%
! TGGGAATGATTTTTACAACT+AGG - chr3_1:151554-151573 Msa0342460:CDS 30.0%
AAAATGTCGTGTTTAGTAAG+GGG + chr3_1:151590-151609 None:intergenic 30.0%
TCACTTATTAGTTCCAGAAA+AGG - chr3_1:151980-151999 Msa0342460:intron 30.0%
! AGTAACTGTTTAACCTTTTC+TGG + chr3_1:151996-152015 None:intergenic 30.0%
! ACATCAAGAAGGATTTTCAT+TGG + chr3_1:152070-152089 None:intergenic 30.0%
TGATGTAAGCTAGTTGAATT+TGG - chr3_1:152084-152103 Msa0342460:intron 30.0%
! AAGAATCAATTAAAGTGCCA+CGG + chr3_1:152180-152199 None:intergenic 30.0%
! TTACTCACTAACCATTTTCT+TGG + chr3_1:152254-152273 None:intergenic 30.0%
TTTATTCTCCAGATCTGTCA+TGG + chr3_1:148594-148613 None:intergenic 35.0%
ATGACAGATCTGGAGAATAA+AGG - chr3_1:148593-148612 Msa0342460:three_prime_UTR 35.0%
ACTTATCATTCAACGGTAGA+AGG + chr3_1:148782-148801 None:intergenic 35.0%
GCAATAACAGCATAACTCTT+GGG + chr3_1:148813-148832 None:intergenic 35.0%
TGCAATAACAGCATAACTCT+TGG + chr3_1:148814-148833 None:intergenic 35.0%
ATTGATCGGAACAATGTCAA+GGG - chr3_1:148966-148985 Msa0342460:intron 35.0%
TACAACATACATCGGAATTC+GGG + chr3_1:149040-149059 None:intergenic 35.0%
! TAGTTCTCGAGGTAAGTTTT+TGG - chr3_1:149226-149245 Msa0342460:intron 35.0%
!! ATCCTTTATGTTTGGTCTTG+GGG - chr3_1:149287-149306 Msa0342460:intron 35.0%
! TGTTGTGGAAATTTAAGGTC+AGG - chr3_1:149367-149386 Msa0342460:intron 35.0%
!!! TCTGTTTTGGTGCTGATATA+AGG - chr3_1:149450-149469 Msa0342460:intron 35.0%
TTGCCTACAAGCTATATTCT+TGG - chr3_1:149528-149547 Msa0342460:intron 35.0%
! GTACCAAGAATATAGCTTGT+AGG + chr3_1:149534-149553 None:intergenic 35.0%
AGAGAGATGTGTTTGTTTAC+TGG - chr3_1:149680-149699 Msa0342460:intron 35.0%
! AAAATAATACGGGTCGGTTT+CGG - chr3_1:149992-150011 Msa0342460:intron 35.0%
GATGGTCAAAATTCATCCAT+CGG + chr3_1:150021-150040 None:intergenic 35.0%
! TGCCAGATTTTGTACAATGA+AGG + chr3_1:150198-150217 None:intergenic 35.0%
AAAATCTGGCACTATAGACT+TGG - chr3_1:150207-150226 Msa0342460:intron 35.0%
! TTTTTGTTCTGCCTGTTGTT+TGG - chr3_1:150306-150325 Msa0342460:intron 35.0%
!! TTTTGTTCTGCCTGTTGTTT+GGG - chr3_1:150307-150326 Msa0342460:intron 35.0%
! TTTTGTGATTTATGAGGACC+TGG - chr3_1:150958-150977 Msa0342460:intron 35.0%
ACGAGAATAGACATTAACAC+AGG + chr3_1:151008-151027 None:intergenic 35.0%
!! TCTATTCTCGTCGTTTTACT+TGG - chr3_1:151017-151036 Msa0342460:intron 35.0%
!! GTGTGAGACTTCAGTTTTAA+AGG + chr3_1:151049-151068 None:intergenic 35.0%
GCCAAGATTGTTGAAATAAG+AGG + chr3_1:151172-151191 None:intergenic 35.0%
!!! ATTTTGATGGTGAGGTATGA+GGG + chr3_1:151213-151232 None:intergenic 35.0%
TCATACCTCACCATCAAAAT+GGG - chr3_1:151213-151232 Msa0342460:intron 35.0%
! TTTCTACAGACCCATTTTGA+TGG + chr3_1:151226-151245 None:intergenic 35.0%
ATCAAAATGGGTCTGTAGAA+AGG - chr3_1:151225-151244 Msa0342460:intron 35.0%
! TGTTTTACATCACGAGCTTA+TGG + chr3_1:151284-151303 None:intergenic 35.0%
GCTCGTGATGTAAAACAATT+AGG - chr3_1:151287-151306 Msa0342460:intron 35.0%
TTCCTGGGAACATTATTCTT+GGG + chr3_1:151343-151362 None:intergenic 35.0%
ATTCCTGGGAACATTATTCT+TGG + chr3_1:151344-151363 None:intergenic 35.0%
AGAAGTGAAATCATAATCCC+GGG - chr3_1:151366-151385 Msa0342460:intron 35.0%
ATGGGAGGTTATTTATTCCT+AGG - chr3_1:151455-151474 Msa0342460:intron 35.0%
CCTGGATATAAAACATTCCT+AGG + chr3_1:151475-151494 None:intergenic 35.0%
! CCTAGGAATGTTTTATATCC+AGG - chr3_1:151472-151491 Msa0342460:intron 35.0%
AAATGTCGTGTTTAGTAAGG+GGG + chr3_1:151589-151608 None:intergenic 35.0%
AGAACATGACCAACAAAGAA+AGG + chr3_1:151751-151770 None:intergenic 35.0%
TTGGTCATGTTCTTCTCTTT+GGG - chr3_1:151758-151777 Msa0342460:CDS 35.0%
! CAAACTAATAACTTTTCCGG+TGG + chr3_1:151934-151953 None:intergenic 35.0%
! TCAACTAGCTTACATCAAGA+AGG + chr3_1:152081-152100 None:intergenic 35.0%
GTTGAATTTGGTCACAATTG+TGG - chr3_1:152096-152115 Msa0342460:intron 35.0%
ACATTGAAATATCTTGGTCG+CGG - chr3_1:152142-152161 Msa0342460:CDS 35.0%
ATTGAACTCTGCCAAGAAAA+TGG - chr3_1:152240-152259 Msa0342460:CDS 35.0%
CACATTGATACAGCTGCTTA+GGG + chr3_1:148508-148527 None:intergenic 40.0%
ACACATTGATACAGCTGCTT+AGG + chr3_1:148509-148528 None:intergenic 40.0%
TGTGTATGCAAAGCAATACC+TGG - chr3_1:148524-148543 Msa0342460:three_prime_UTR 40.0%
AGCAATACCTGGAAGAGTTA+TGG - chr3_1:148535-148554 Msa0342460:three_prime_UTR 40.0%
CTACCGTTGAATGATAAGTC+TGG - chr3_1:148783-148802 Msa0342460:three_prime_UTR 40.0%
! TGCATCTGAAATCAAGGAGT+TGG - chr3_1:148830-148849 Msa0342460:three_prime_UTR 40.0%
GATTGATCGGAACAATGTCA+AGG - chr3_1:148965-148984 Msa0342460:intron 40.0%
CTTGAATCTAGGTCGAATGA+GGG + chr3_1:149122-149141 None:intergenic 40.0%
! CTTCTTTGCTGCTTGAATCT+AGG + chr3_1:149133-149152 None:intergenic 40.0%
AACCCCAAGACCAAACATAA+AGG + chr3_1:149292-149311 None:intergenic 40.0%
! TGGGGTTGTGTTTTTCTGTT+CGG - chr3_1:149305-149324 Msa0342460:intron 40.0%
!! TGGTGCTGATATAAGGAGTT+CGG - chr3_1:149457-149476 Msa0342460:intron 40.0%
!! TAGTTGGTACTGGTAGTACT+TGG - chr3_1:149804-149823 Msa0342460:intron 40.0%
CCAACTAGTGTAACTTGGAA+AGG + chr3_1:149900-149919 None:intergenic 40.0%
CCTTTCCAAGTTACACTAGT+TGG - chr3_1:149897-149916 Msa0342460:intron 40.0%
!! GGTTGTCGGTTGAACATTTT+TGG + chr3_1:150073-150092 None:intergenic 40.0%
! AAATGACAAACAGGAGGTGT+AGG + chr3_1:150100-150119 None:intergenic 40.0%
!!! ATTCGGTTTTTTTCGGGTTC+AGG - chr3_1:150125-150144 Msa0342460:intron 40.0%
!!! TTCGGTTTTTTTCGGGTTCA+GGG - chr3_1:150126-150145 Msa0342460:intron 40.0%
! TTTGTTCTGCCTGTTGTTTG+GGG - chr3_1:150308-150327 Msa0342460:intron 40.0%
GTGGCAGTAGAAGTAAAAGA+GGG + chr3_1:150349-150368 None:intergenic 40.0%
AGTGGCAGTAGAAGTAAAAG+AGG + chr3_1:150350-150369 None:intergenic 40.0%
CTCGTATGAGTGAAATGTGT+CGG + chr3_1:150543-150562 None:intergenic 40.0%
TAACCTCCAAAAGACTCTAC+TGG + chr3_1:150985-151004 None:intergenic 40.0%
ATCAGTCTGAACAGACTTGA+TGG + chr3_1:151124-151143 None:intergenic 40.0%
ATCAAGTCTGTTCAGACTGA+TGG - chr3_1:151123-151142 Msa0342460:intron 40.0%
!!! CATTTTGATGGTGAGGTATG+AGG + chr3_1:151214-151233 None:intergenic 40.0%
CTCATACCTCACCATCAAAA+TGG - chr3_1:151212-151231 Msa0342460:intron 40.0%
!! AATTAGGGGCGTTTGTTTCT+TGG - chr3_1:151303-151322 Msa0342460:intron 40.0%
TCCTGGGAACATTATTCTTG+GGG + chr3_1:151342-151361 None:intergenic 40.0%
!!! GCTTCTTTTGAAAAGTACCC+GGG + chr3_1:151386-151405 None:intergenic 40.0%
!!! CGCTTCTTTTGAAAAGTACC+CGG + chr3_1:151387-151406 None:intergenic 40.0%
TTGTAAACATGGGAAATGGG+AGG - chr3_1:151440-151459 Msa0342460:intron 40.0%
GTTGGTCATGTTCTTCTCTT+TGG - chr3_1:151757-151776 Msa0342460:CDS 40.0%
! AATTAAAGTGCCACGGAAAC+AGG + chr3_1:152173-152192 None:intergenic 40.0%
! AACGTTGGCACTGAGTATCT+TGG - chr3_1:148563-148582 Msa0342460:three_prime_UTR 45.0%
AGATCTGGAGAATAAAGGCC+TGG - chr3_1:148598-148617 Msa0342460:three_prime_UTR 45.0%
! AATGTCAAGGGCATGGTGAT+TGG - chr3_1:148978-148997 Msa0342460:intron 45.0%
GCTTGAATCTAGGTCGAATG+AGG + chr3_1:149123-149142 None:intergenic 45.0%
ACTTACCTCGAGAACTAGCT+GGG + chr3_1:149223-149242 None:intergenic 45.0%
AACTTACCTCGAGAACTAGC+TGG + chr3_1:149224-149243 None:intergenic 45.0%
! GTGTTTTTCTGTTCGGACTG+AGG - chr3_1:149312-149331 Msa0342460:intron 45.0%
! TTTTTCTGTTCGGACTGAGG+AGG - chr3_1:149315-149334 Msa0342460:intron 45.0%
!! GTTCCGTAGTTCCTGCTTTT+AGG - chr3_1:149416-149435 Msa0342460:intron 45.0%
ACGCCTAAAAGCAGGAACTA+CGG + chr3_1:149422-149441 None:intergenic 45.0%
CTAGTTACACGCCTAAAAGC+AGG + chr3_1:149430-149449 None:intergenic 45.0%
!! GGCGTGTAACTAGTCTGTTT+TGG - chr3_1:149437-149456 Msa0342460:intron 45.0%
AACGGCCAACTAGTGTAACT+TGG + chr3_1:149905-149924 None:intergenic 45.0%
TACACTAGTTGGCCGTTGTT+CGG - chr3_1:149908-149927 Msa0342460:intron 45.0%
AGATATGAAGGGGTGATGTG+AGG + chr3_1:149960-149979 None:intergenic 45.0%
TAGTAACAGTCTAGTGCGGA+TGG + chr3_1:150039-150058 None:intergenic 45.0%
TGGGTAGTAACAGTCTAGTG+CGG + chr3_1:150043-150062 None:intergenic 45.0%
! ACAAACAGGAGGTGTAGGAT+CGG + chr3_1:150095-150114 None:intergenic 45.0%
! TTGAACAGAATCTCTAGGGG+TGG + chr3_1:150170-150189 None:intergenic 45.0%
GACTTGGACATCTCACTATG+AGG - chr3_1:150223-150242 Msa0342460:intron 45.0%
ATGAGCTTTCCCCAAACAAC+AGG + chr3_1:150320-150339 None:intergenic 45.0%
ACGTGTCGGACAACTAAGAA+TGG - chr3_1:150466-150485 Msa0342460:intron 45.0%
AAATGTGTCGGACACGATCT+AGG + chr3_1:150531-150550 None:intergenic 45.0%
TTCACTCATACGAGTCGTGT+CGG - chr3_1:150549-150568 Msa0342460:intron 45.0%
ACTCTTGTCTCTACGAAGTG+TGG + chr3_1:150620-150639 None:intergenic 45.0%
ATGAAGCACAGACTCCAACA+CGG + chr3_1:150917-150936 None:intergenic 45.0%
AAGTCTGTTCAGACTGATGG+TGG - chr3_1:151126-151145 Msa0342460:intron 45.0%
!!! ACAGACCCATTTTGATGGTG+AGG + chr3_1:151221-151240 None:intergenic 45.0%
! GGTCTGTAGAAAGGAAGCAT+AGG - chr3_1:151234-151253 Msa0342460:intron 45.0%
TCCCCAAGAATAATGTTCCC+AGG - chr3_1:151338-151357 Msa0342460:intron 45.0%
GTTCCTGAATACCGTTCACA+AGG - chr3_1:151891-151910 Msa0342460:intron 45.0%
CTTCCTTGTGAACGGTATTC+AGG + chr3_1:151897-151916 None:intergenic 45.0%
GAGCTAGACTTCCTTGTGAA+CGG + chr3_1:151905-151924 None:intergenic 45.0%
!! AAAAAAAAAAAAAACATTAA+CGG - chr3_1:148737-148756 Msa0342460:three_prime_UTR 5.0%
!! AAAAATGAATTTATTAATTT+AGG + chr3_1:149589-149608 None:intergenic 5.0%
!! ATAAAAAATTAAAATAATAC+GGG - chr3_1:149982-150001 Msa0342460:intron 5.0%
TTGAGCGCCATAACTCTTCC+AGG + chr3_1:148545-148564 None:intergenic 50.0%
AGAGTTATGGCGCTCAACGT+TGG - chr3_1:148548-148567 Msa0342460:three_prime_UTR 50.0%
TGGAGTTGCCATGACAGATC+TGG - chr3_1:148583-148602 Msa0342460:three_prime_UTR 50.0%
TCTGGAGAATAAAGGCCTGG+AGG - chr3_1:148601-148620 Msa0342460:three_prime_UTR 50.0%
ATAAAGGCCTGGAGGACTCT+AGG - chr3_1:148609-148628 Msa0342460:three_prime_UTR 50.0%
TCGGAACAATGTCAAGGGCA+TGG - chr3_1:148971-148990 Msa0342460:intron 50.0%
GGGCATGGTGATTGGAAATC+TGG - chr3_1:148986-149005 Msa0342460:intron 50.0%
GACGACTATCGAGCTCTTCA+AGG + chr3_1:149013-149032 None:intergenic 50.0%
CATACATCGGAATTCGGGAG+AGG + chr3_1:149035-149054 None:intergenic 50.0%
GAATCTAGGTCGAATGAGGG+AGG + chr3_1:149119-149138 None:intergenic 50.0%
TGAGGAGGATGCATCAGTGT+TGG - chr3_1:149330-149349 Msa0342460:intron 50.0%
! AAGGAGTTCGGTTTGCTCCT+TGG - chr3_1:149469-149488 Msa0342460:intron 50.0%
!! TCGGTTTGCTCCTTGGTGTT+AGG - chr3_1:149476-149495 Msa0342460:intron 50.0%
!! CGGTTTGCTCCTTGGTGTTA+GGG - chr3_1:149477-149496 Msa0342460:intron 50.0%
!! GGTTTGCTCCTTGGTGTTAG+GGG - chr3_1:149478-149497 Msa0342460:intron 50.0%
AAACTCCACCCCTAACACCA+AGG + chr3_1:149489-149508 None:intergenic 50.0%
!! GTACTTGGTAGCGTGTTTGC+AGG - chr3_1:149819-149838 Msa0342460:intron 50.0%
ACACTAGTTGGCCGTTGTTC+GGG - chr3_1:149909-149928 Msa0342460:intron 50.0%
! CAAACAGGAGGTGTAGGATC+GGG + chr3_1:150094-150113 None:intergenic 50.0%
!!! GTTTTTTTCGGGTTCAGGGC+AGG - chr3_1:150130-150149 Msa0342460:intron 50.0%
!! GCATGGACACCTCTAAGACT+AGG - chr3_1:150386-150405 Msa0342460:intron 50.0%
!! CTCTAAGACTAGGCGTGTCA+CGG - chr3_1:150396-150415 Msa0342460:intron 50.0%
GATACTCGTACGACACGTGT+CGG - chr3_1:150452-150471 Msa0342460:intron 50.0%
CCAACACGGACATTTCGACA+CGG + chr3_1:150903-150922 None:intergenic 50.0%
CCAAAAGACTCTACTGGACC+AGG + chr3_1:150979-150998 None:intergenic 50.0%
! CCTGGTCCAGTAGAGTCTTT+TGG - chr3_1:150976-150995 Msa0342460:intron 50.0%
! GGTCCAGTAGAGTCTTTTGG+AGG - chr3_1:150979-150998 Msa0342460:intron 50.0%
!! TCTGTTCAGACTGATGGTGG+TGG - chr3_1:151129-151148 Msa0342460:intron 50.0%
! AGTCTAGCTCATAGCACCAC+CGG - chr3_1:151915-151934 Msa0342460:intron 50.0%
ACCTACTCCTAGAGTCCTCC+AGG + chr3_1:148619-148638 None:intergenic 55.0%
TCGAGAACTAGCTGGGCTCT+CGG + chr3_1:149216-149235 None:intergenic 55.0%
!! TTGCTCCTTGGTGTTAGGGG+TGG - chr3_1:149481-149500 Msa0342460:intron 55.0%
GGTAGCGTGTTTGCAGGTGA+AGG - chr3_1:149825-149844 Msa0342460:intron 55.0%
ACCACACACAGCCCGAACAA+CGG + chr3_1:149923-149942 None:intergenic 55.0%
GAAGGGGTGATGTGAGGACT+CGG + chr3_1:149954-149973 None:intergenic 55.0%
! GAGGTGTAGGATCGGGTTGT+CGG + chr3_1:150087-150106 None:intergenic 55.0%
!!! TTTTTCGGGTTCAGGGCAGG+TGG - chr3_1:150133-150152 Msa0342460:intron 55.0%
!! CGTGACACGCCTAGTCTTAG+AGG + chr3_1:150398-150417 None:intergenic 55.0%
TGTCGTAGACCGACGCGTAT+CGG + chr3_1:150425-150444 None:intergenic 55.0%
CCGTGTCGAAATGTCCGTGT+TGG - chr3_1:150900-150919 Msa0342460:intron 55.0%
! TGATGGTGAGGTATGAGGGC+AGG + chr3_1:151209-151228 None:intergenic 55.0%
! GCCTGGAGGACTCTAGGAGT+AGG - chr3_1:148615-148634 Msa0342460:three_prime_UTR 60.0%
AGAACTAGCTGGGCTCTCGG+TGG + chr3_1:149213-149232 None:intergenic 60.0%
! GAGAGCCCAGCTAGTTCTCG+AGG - chr3_1:149215-149234 Msa0342460:intron 60.0%
! GGGTCGGTTTCGGTTTCCGA+TGG - chr3_1:150002-150021 Msa0342460:intron 60.0%
TCACGGTGTCCGATACGCGT+CGG - chr3_1:150413-150432 Msa0342460:intron 60.0%
CGCGGTACATCCTGTTTCCG+TGG - chr3_1:152160-152179 Msa0342460:CDS 60.0%
GCCGTTGTTCGGGCTGTGTG+TGG - chr3_1:149919-149938 Msa0342460:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 148463 152313 148463 ID=Msa0342460;Name=Msa0342460
chr3_1 mRNA 148463 152313 148463 ID=Msa0342460-mRNA-1;Parent=Msa0342460;Name=Msa0342460-mRNA-1;_AED=0.10;_eAED=0.10;_QI=28|1|1|1|0.75|1|5|392|145
chr3_1 exon 148463 148887 148463 ID=Msa0342460-mRNA-1:exon:17;Parent=Msa0342460-mRNA-1
chr3_1 exon 151540 151641 151540 ID=Msa0342460-mRNA-1:exon:16;Parent=Msa0342460-mRNA-1
chr3_1 exon 151727 151810 151727 ID=Msa0342460-mRNA-1:exon:15;Parent=Msa0342460-mRNA-1
chr3_1 exon 151925 151997 151925 ID=Msa0342460-mRNA-1:exon:14;Parent=Msa0342460-mRNA-1
chr3_1 exon 152140 152313 152140 ID=Msa0342460-mRNA-1:exon:13;Parent=Msa0342460-mRNA-1
chr3_1 five_prime_UTR 152286 152313 152286 ID=Msa0342460-mRNA-1:five_prime_utr;Parent=Msa0342460-mRNA-1
chr3_1 CDS 152140 152285 152140 ID=Msa0342460-mRNA-1:cds;Parent=Msa0342460-mRNA-1
chr3_1 CDS 151925 151997 151925 ID=Msa0342460-mRNA-1:cds;Parent=Msa0342460-mRNA-1
chr3_1 CDS 151727 151810 151727 ID=Msa0342460-mRNA-1:cds;Parent=Msa0342460-mRNA-1
chr3_1 CDS 151540 151641 151540 ID=Msa0342460-mRNA-1:cds;Parent=Msa0342460-mRNA-1
chr3_1 CDS 148855 148887 148855 ID=Msa0342460-mRNA-1:cds;Parent=Msa0342460-mRNA-1
chr3_1 three_prime_UTR 148463 148854 148463 ID=Msa0342460-mRNA-1:three_prime_utr;Parent=Msa0342460-mRNA-1
Gene Sequence

>Msa0342460

ATGAAGCACATGCTCCCTAAGCAGCTGTATCAATGTGTATGCAAAGCAATACCTGGAAGAGTTATGGCGCTCAACGTTGGCACTGAGTATCTTGGAGTTGCCATGACAGATCTGGAGAATAAAGGCCTGGAGGACTCTAGGAGTAGCCTTCTACCGTTGAATGATAAGTCTGGAAAGCCCAAGAGTTATGCTGTTATTGCATCTGAAATCAAGGAGTTGATTGATCGGAACAATGTCAAGGGCATGGTGATTGGAAATCTGGACCTTGAAGAGCTCGATAGTCGTCCTCTCCCGAATTCCGATATTCAAGCAGCAAAGAAGTTCGTCCAAAAGCTTGCGAAAACAAATATTATCAGTGATATGAACTATACATTGCTTCCACCGAGAGCCCAGCTAGTTCTCGAGAAGTTCCTGAATACCGTTCACAAGGAAGTCTAG
Protein sequence

>Msa0342460

MKHMLPKQLYQCVCKAIPGRVMALNVGTEYLGVAMTDLENKGLEDSRSSLLPLNDKSGKPKSYAVIASEIKELIDRNNVKGMVIGNLDLEELDSRPLPNSDIQAAKKFVQKLAKTNIISDMNYTLLPPRAQLVLEKFLNTVHKEV*