AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0361130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 95 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTGTGGCAGCAGCTATTT+TGG 0.096788 3_1:-51341034 None:intergenic
AGCAAAACTTGAACAGATTT+TGG 0.146646 3_1:-51341908 None:intergenic
GGATCTCTCTGCAACAAATT+AGG 0.185049 3_1:-51340955 None:intergenic
GATTGAATTGCAGGGTAATT+TGG 0.204017 3_1:+51344069 Msa0361130:exon
AAACCTCACTGATATCATTT+TGG 0.206558 3_1:+51341770 Msa0361130:five_prime_UTR
TTGTAAGATTTATTGATTTC+AGG 0.218492 3_1:-51341229 None:intergenic
AGGTTCATTCTGTGAGATTT+TGG 0.222633 3_1:+51341719 Msa0361130:five_prime_UTR
TACTTTGTGGTTGAGCTAAA+AGG 0.236133 3_1:-51341125 None:intergenic
CCTTACACGGGGACCAAGTT+TGG 0.247711 3_1:-51343908 None:intergenic
TTACTGTTGAATTCTGATTA+TGG 0.256105 3_1:+51341271 Msa0361130:five_prime_UTR
TTAGATTACTTTCCATATTT+AGG 0.260725 3_1:-51342080 None:intergenic
TATTTCAGGGTGGGCTTTGA+TGG 0.281036 3_1:+51343862 Msa0361130:intron
TGGGTGGTTGATTGAATTGC+AGG 0.284576 3_1:+51344060 Msa0361130:exon
CGAACACAGCAACCAGCTTT+AGG 0.295367 3_1:-51341667 None:intergenic
TATTTAGGTCCTAATCCTTC+TGG 0.311577 3_1:-51342065 None:intergenic
GATCTCTCTGCAACAAATTA+GGG 0.345768 3_1:-51340954 None:intergenic
GAAGGATTAGGACCTAAATA+TGG 0.361824 3_1:+51342068 Msa0361130:exon
TAATGGAGCTACTGTATTGC+AGG 0.368010 3_1:+51342119 Msa0361130:CDS
GAATCTACACACACAAGAAA+TGG 0.396244 3_1:-51344190 None:intergenic
ATCTTGGTGATGGCACTATA+CGG 0.403828 3_1:-51340992 None:intergenic
AAAATTTGCTAGGTACTTTG+TGG 0.408513 3_1:-51341138 None:intergenic
AATACAGTATTGTATACTAA+TGG 0.410307 3_1:+51342102 Msa0361130:CDS
CTATACGGCCAAGTTAAATC+AGG 0.415110 3_1:-51340977 None:intergenic
TCCAGGACTGTTCCCAAACT+TGG 0.417969 3_1:+51343895 Msa0361130:CDS
TGGTTGATCAGGTTTCAAAA+AGG 0.418460 3_1:-51341888 None:intergenic
CTTACACGGGGACCAAGTTT+GGG 0.420704 3_1:-51343907 None:intergenic
ACTTTGTGGTTGAGCTAAAA+GGG 0.430585 3_1:-51341124 None:intergenic
TGGTTGCTGTGTTCGTTGTT+TGG 0.436224 3_1:+51341675 Msa0361130:five_prime_UTR
TGAAGAGCCTGTAGAATGTC+AGG 0.442841 3_1:-51343741 None:intergenic
GAGTTTCGACAAACAATCAA+TGG 0.446006 3_1:-51341822 None:intergenic
CCAAACTTGGTCCCCGTGTA+AGG 0.448709 3_1:+51343908 Msa0361130:CDS
CGAAACTCTGATGTGGTTCA+TGG 0.449538 3_1:+51341837 Msa0361130:five_prime_UTR
AATTGCAGGGTAATTTGGAA+TGG 0.457236 3_1:+51344074 Msa0361130:exon
ACCAAGTTTGGGAACAGTCC+TGG 0.463033 3_1:-51343896 None:intergenic
CAGGCTCTTCAACTGAAGAA+TGG 0.470883 3_1:+51343753 Msa0361130:CDS
CAAGGCTGACCTGAGAATCT+TGG 0.471621 3_1:-51341008 None:intergenic
GTGCCATCACCAAGATTCTC+AGG 0.481675 3_1:+51340999 Msa0361130:five_prime_UTR
AGCAGGTTTCAATTTGTCAC+TGG 0.482750 3_1:+51341638 Msa0361130:five_prime_UTR
AGGCTCTTCAACTGAAGAAT+GGG 0.483358 3_1:+51343754 Msa0361130:CDS
TAGGGCTTCTTTCTTCTCAA+AGG 0.496253 3_1:-51340936 None:intergenic
GAGAGATCCCTGATTTAACT+TGG 0.500916 3_1:+51340969 Msa0361130:five_prime_UTR
TCCATTGTAAGCTGCTGAAA+TGG 0.501948 3_1:-51341073 None:intergenic
CATCAAAAGACAACCTTACA+CGG 0.504771 3_1:-51343921 None:intergenic
ATTGCAGGGTAATTTGGAAT+GGG 0.505588 3_1:+51344075 Msa0361130:three_prime_UTR
GTTTGTGATCCGAGCTGAGC+AGG 0.507962 3_1:+51341621 Msa0361130:five_prime_UTR
TTGATGGAGTTGAGACATCC+AGG 0.509692 3_1:+51343878 Msa0361130:CDS
TAGGTAGTAGACAACTGGTT+GGG 0.511261 3_1:+51341161 Msa0361130:five_prime_UTR
GATCAATCCTGACATTCTAC+AGG 0.516099 3_1:+51343734 Msa0361130:CDS
AGTGATTGTGTACATCTAAA+GGG 0.517240 3_1:+51344099 Msa0361130:three_prime_UTR
CGCAGAAATTGTCGAGGGGA+GGG 0.517709 3_1:+51341539 Msa0361130:five_prime_UTR
TGAATATGACTGTGGTTGGG+TGG 0.517723 3_1:+51344044 Msa0361130:CDS
AAGTGATTGTGTACATCTAA+AGG 0.527661 3_1:+51344098 Msa0361130:three_prime_UTR
AAGGACAATAAGTGTGCTGT+TGG 0.535488 3_1:-51344380 None:intergenic
AGATTTATTGATTTCAGGTG+AGG 0.535867 3_1:-51341224 None:intergenic
GGGTGGTTGATTGAATTGCA+GGG 0.539536 3_1:+51344061 Msa0361130:exon
GAACCATCAGAAAATTCTAA+AGG 0.539845 3_1:+51341187 Msa0361130:five_prime_UTR
TGTGGTAGTATGAGTTGTTG+AGG 0.540068 3_1:+51344134 Msa0361130:three_prime_UTR
GATTTATTGATTTCAGGTGA+GGG 0.542797 3_1:-51341223 None:intergenic
GTTATGAATATGACTGTGGT+TGG 0.544198 3_1:+51344040 Msa0361130:CDS
TTTCAGGTGAGGGGAAGAGT+TGG 0.547237 3_1:-51341213 None:intergenic
GTGTACATCTAAAGGGTGAT+GGG 0.547948 3_1:+51344106 Msa0361130:three_prime_UTR
ACCATTTCAGCAGCTTACAA+TGG 0.554176 3_1:+51341072 Msa0361130:five_prime_UTR
ATTAGATACTTAGATACTGC+AGG 0.556284 3_1:-51344294 None:intergenic
CTCACATGATTACTATGTGA+TGG 0.564330 3_1:-51341097 None:intergenic
AATAGCTGCTGCCACACAGA+TGG 0.575125 3_1:+51341038 Msa0361130:five_prime_UTR
TATATAGTCCTCAAAACAGA+GGG 0.576602 3_1:-51341591 None:intergenic
TGTGTACATCTAAAGGGTGA+TGG 0.582679 3_1:+51344105 Msa0361130:three_prime_UTR
AAAGGGTGATGGGATTATTG+TGG 0.583866 3_1:+51344116 Msa0361130:three_prime_UTR
TTATGAATATGACTGTGGTT+GGG 0.585778 3_1:+51344041 Msa0361130:CDS
TTGGTTAAGAAATTTATGCA+TGG 0.589947 3_1:-51344361 None:intergenic
TCGCAGAAATTGTCGAGGGG+AGG 0.597851 3_1:+51341538 Msa0361130:five_prime_UTR
AAATTGAAACCTGCTCAGCT+CGG 0.599524 3_1:-51341630 None:intergenic
TTAGGTAGTAGACAACTGGT+TGG 0.600091 3_1:+51341160 Msa0361130:five_prime_UTR
ATCAAAAGACAACCTTACAC+GGG 0.602605 3_1:-51343920 None:intergenic
TATATCAGTCAGCTGAGCAA+TGG 0.609649 3_1:-51343974 None:intergenic
ATTTATTGATTTCAGGTGAG+GGG 0.610622 3_1:-51341222 None:intergenic
TGACCTGAGAATCTTGGTGA+TGG 0.617792 3_1:-51341002 None:intergenic
CCTGAGAAATGGAAGGAGCA+AGG 0.619572 3_1:+51340893 None:intergenic
TAGTAGACAACTGGTTGGGA+AGG 0.622406 3_1:+51341165 Msa0361130:five_prime_UTR
TGTTTGTCGAAACTCTGATG+TGG 0.625592 3_1:+51341830 Msa0361130:five_prime_UTR
AGAATGCGATGAATATAACT+AGG 0.626097 3_1:+51343706 Msa0361130:CDS
TTCATCGCAGAAATTGTCGA+GGG 0.626679 3_1:+51341534 Msa0361130:five_prime_UTR
TGGTTCATGGTAGAGAGACA+TGG 0.636010 3_1:+51341850 Msa0361130:five_prime_UTR
TATACGGCCAAGTTAAATCA+GGG 0.640400 3_1:-51340976 None:intergenic
CTTTGTGGTTGAGCTAAAAG+GGG 0.643100 3_1:-51341123 None:intergenic
TTATATAGTCCTCAAAACAG+AGG 0.669442 3_1:-51341592 None:intergenic
ATTCATCGCAGAAATTGTCG+AGG 0.682405 3_1:+51341533 Msa0361130:five_prime_UTR
CTGACTGATATAGCTGACGA+TGG 0.686698 3_1:+51343985 Msa0361130:CDS
GAGAGTTATGAATATGACTG+TGG 0.698344 3_1:+51344036 Msa0361130:CDS
TCATCGCAGAAATTGTCGAG+GGG 0.709533 3_1:+51341535 Msa0361130:five_prime_UTR
GAACCAAAATGATATCAGTG+AGG 0.723766 3_1:-51341773 None:intergenic
TCAAAAGACAACCTTACACG+GGG 0.740383 3_1:-51343919 None:intergenic
TTCATGGTAGAGAGACATGG+AGG 0.745685 3_1:+51341853 Msa0361130:five_prime_UTR
AGATGAGAACACCATCTGTG+TGG 0.761405 3_1:-51341049 None:intergenic
ATATAGTCCTCAAAACAGAG+GGG 0.770520 3_1:-51341590 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTGAAGAATTTTTATTT+AGG + chr3_1:51342445-51342464 Msa0361130:CDS 15.0%
!!! TTGTTTATGTCATTTTTTAA+AGG + chr3_1:51342574-51342593 Msa0361130:intron 15.0%
!!! ATTTTATTTTTGTTTCTATG+AGG - chr3_1:51342855-51342874 None:intergenic 15.0%
!!! TTAATACTAAAAAGTATATG+CGG - chr3_1:51342926-51342945 None:intergenic 15.0%
!! ATTGTATATCTATATAACTT+CGG + chr3_1:51343787-51343806 Msa0361130:intron 15.0%
!! ATAAGTAAAAGAAAAAGAAA+AGG - chr3_1:51344402-51344421 None:intergenic 15.0%
!!! TTGTAAGATTTATTGATTTC+AGG - chr3_1:51341232-51341251 None:intergenic 20.0%
!! AAAAAATTTGAATTTCCTGT+CGG - chr3_1:51341449-51341468 None:intergenic 20.0%
!!! TCAAATTTTTTGTTTGTTTG+TGG + chr3_1:51341458-51341477 Msa0361130:intron 20.0%
!!! TTCATTTTGTTATTCATACT+TGG + chr3_1:51341953-51341972 Msa0361130:intron 20.0%
!! TTAGATTACTTTCCATATTT+AGG - chr3_1:51342083-51342102 None:intergenic 20.0%
!! AATACAGTATTGTATACTAA+TGG + chr3_1:51342102-51342121 Msa0361130:CDS 20.0%
!!! CTTAATCCTTTGTTTTTATT+TGG + chr3_1:51342360-51342379 Msa0361130:intron 20.0%
!! TACTAACCAAATAAAAACAA+AGG - chr3_1:51342369-51342388 None:intergenic 20.0%
!! TTATAGCTACATATGAAAAA+TGG + chr3_1:51342491-51342510 Msa0361130:intron 20.0%
!! ATAATTTATGCAATTCAAGA+AGG + chr3_1:51342517-51342536 Msa0361130:intron 20.0%
!! TGACATAAACAAATAATAAG+AGG - chr3_1:51342566-51342585 None:intergenic 20.0%
!! ACTAAAAAGAAGAAGAAAAA+AGG - chr3_1:51342782-51342801 None:intergenic 20.0%
!! TGTTTCAATAGTTAACAATT+TGG - chr3_1:51343394-51343413 None:intergenic 20.0%
!!! ACAGATTTTTGCAAAAAAAA+AGG - chr3_1:51343477-51343496 None:intergenic 20.0%
!! CAACATAAAAATACCAAAAT+AGG - chr3_1:51344254-51344273 None:intergenic 20.0%
!!! AAAGTACCTAGCAAATTTTT+AGG + chr3_1:51341142-51341161 Msa0361130:five_prime_UTR 25.0%
!!! TTACCTTTAGAATTTTCTGA+TGG - chr3_1:51341193-51341212 None:intergenic 25.0%
! TTACTGTTGAATTCTGATTA+TGG + chr3_1:51341271-51341290 Msa0361130:five_prime_UTR 25.0%
!! TCTCATTCTTTTTGCTTAAA+GGG + chr3_1:51341341-51341360 Msa0361130:intron 25.0%
!!! AGGTTTGTTTCAAACTTTTA+AGG + chr3_1:51341501-51341520 Msa0361130:intron 25.0%
!!! GTTTCAAACTTTTAAGGATT+TGG + chr3_1:51341507-51341526 Msa0361130:five_prime_UTR 25.0%
!!! ACATACATCAATTGTTTTTG+TGG - chr3_1:51342042-51342061 None:intergenic 25.0%
!!! GAAGAATTTTTATTTAGGTG+TGG + chr3_1:51342450-51342469 Msa0361130:intron 25.0%
! ACAAATAATAAGAGGTTCTA+CGG - chr3_1:51342558-51342577 None:intergenic 25.0%
! TTGATTGTGTCAAACAAAAT+TGG - chr3_1:51342674-51342693 None:intergenic 25.0%
! ACAATTGTTGATCTTAATAG+AGG + chr3_1:51342732-51342751 Msa0361130:intron 25.0%
!!! TTCTTCTTCTTTTTAGTATG+AGG + chr3_1:51342785-51342804 Msa0361130:intron 25.0%
! CATTTGTACATGCAAAAAAA+TGG - chr3_1:51342820-51342839 None:intergenic 25.0%
! ATTCCAATTATGAATTTCAG+AGG + chr3_1:51342873-51342892 Msa0361130:intron 25.0%
!! ATAACTAGCATTGAATTTCA+TGG + chr3_1:51343103-51343122 Msa0361130:intron 25.0%
! ATTCAAACTCATTATCTAGA+AGG - chr3_1:51343160-51343179 None:intergenic 25.0%
!! GAAATTTAAAATACATGACG+TGG - chr3_1:51343290-51343309 None:intergenic 25.0%
! TTTCACTTGATTGTTTAACT+TGG + chr3_1:51343433-51343452 Msa0361130:intron 25.0%
! TTACTGTAAATATTTGCATC+TGG + chr3_1:51343595-51343614 Msa0361130:intron 25.0%
! TACTGTAAATATTTGCATCT+GGG + chr3_1:51343596-51343615 Msa0361130:intron 25.0%
! TAACAAAAGTAATATCAGTG+AGG - chr3_1:51343645-51343664 None:intergenic 25.0%
!!! TAGATTCAGTTGAGTTTTTT+TGG + chr3_1:51344206-51344225 Msa0361130:three_prime_UTR 25.0%
!!! ATTCTTTTTTCCTCCTATTT+TGG + chr3_1:51344238-51344257 Msa0361130:three_prime_UTR 25.0%
! CATAAAAATACCAAAATAGG+AGG - chr3_1:51344251-51344270 None:intergenic 25.0%
! TTGGTTAAGAAATTTATGCA+TGG - chr3_1:51344364-51344383 None:intergenic 25.0%
!! AAAATTTGCTAGGTACTTTG+TGG - chr3_1:51341141-51341160 None:intergenic 30.0%
! CTACTACCTAAAAATTTGCT+AGG - chr3_1:51341151-51341170 None:intergenic 30.0%
!!! ATTTTTAGGTAGTAGACAAC+TGG + chr3_1:51341156-51341175 Msa0361130:five_prime_UTR 30.0%
GAACCATCAGAAAATTCTAA+AGG + chr3_1:51341187-51341206 Msa0361130:five_prime_UTR 30.0%
!! ATTTATTGATTTCAGGTGAG+GGG - chr3_1:51341225-51341244 None:intergenic 30.0%
!! GATTTATTGATTTCAGGTGA+GGG - chr3_1:51341226-51341245 None:intergenic 30.0%
!! AGATTTATTGATTTCAGGTG+AGG - chr3_1:51341227-51341246 None:intergenic 30.0%
!! GCGTCTTCGTTTAAAATTTT+TGG + chr3_1:51341299-51341318 Msa0361130:five_prime_UTR 30.0%
! GTCTCATTCTTTTTGCTTAA+AGG + chr3_1:51341340-51341359 Msa0361130:intron 30.0%
! TTTGTCTTTTTCCCTTTTCA+TGG + chr3_1:51341373-51341392 Msa0361130:intron 30.0%
! AACAAACCTGCTATTTATCA+AGG - chr3_1:51341490-51341509 None:intergenic 30.0%
TATATAGTCCTCAAAACAGA+GGG - chr3_1:51341594-51341613 None:intergenic 30.0%
TTATATAGTCCTCAAAACAG+AGG - chr3_1:51341595-51341614 None:intergenic 30.0%
!!! TCTGTTTTGAGGACTATATA+AGG + chr3_1:51341594-51341613 Msa0361130:five_prime_UTR 30.0%
! TTCATCGTCAATTGAGTTTT+AGG + chr3_1:51341699-51341718 Msa0361130:five_prime_UTR 30.0%
! AAACCTCACTGATATCATTT+TGG + chr3_1:51341770-51341789 Msa0361130:five_prime_UTR 30.0%
! TTCTGATTTTGAATTTGCGA+TGG + chr3_1:51341793-51341812 Msa0361130:five_prime_UTR 30.0%
! AGCAAAACTTGAACAGATTT+TGG - chr3_1:51341911-51341930 None:intergenic 30.0%
CACAAGTACATAATTCAAAG+AGG - chr3_1:51341989-51342008 None:intergenic 30.0%
TACCCTCTGTTATTGTTATA+GGG + chr3_1:51342014-51342033 Msa0361130:intron 30.0%
! ATTGATGTATGTTTTCCAGA+AGG + chr3_1:51342050-51342069 Msa0361130:five_prime_UTR 30.0%
CATGTTTGTGAAATTGCATA+AGG + chr3_1:51342230-51342249 Msa0361130:intron 30.0%
! TCTTCAACTTGAATGCTTTT+AGG - chr3_1:51342435-51342454 None:intergenic 30.0%
!! AGACCTCTGAAATTCATAAT+TGG - chr3_1:51342879-51342898 None:intergenic 30.0%
ATAACACAAGATAGAAATCG+AGG + chr3_1:51343178-51343197 Msa0361130:intron 30.0%
AAACACCAATTTAGTGAGTT+TGG + chr3_1:51343409-51343428 Msa0361130:intron 30.0%
GAAAACCAAACTCACTAAAT+TGG - chr3_1:51343417-51343436 None:intergenic 30.0%
TATTGTATGAGTTGCAGATA+AGG + chr3_1:51343553-51343572 Msa0361130:intron 30.0%
AGAATGCGATGAATATAACT+AGG + chr3_1:51343706-51343725 Msa0361130:CDS 30.0%
TTATGAATATGACTGTGGTT+GGG + chr3_1:51344041-51344060 Msa0361130:CDS 30.0%
AAGTGATTGTGTACATCTAA+AGG + chr3_1:51344098-51344117 Msa0361130:three_prime_UTR 30.0%
AGTGATTGTGTACATCTAAA+GGG + chr3_1:51344099-51344118 Msa0361130:three_prime_UTR 30.0%
ATTAGATACTTAGATACTGC+AGG - chr3_1:51344297-51344316 None:intergenic 30.0%
GATCTCTCTGCAACAAATTA+GGG - chr3_1:51340957-51340976 None:intergenic 35.0%
TATACGGCCAAGTTAAATCA+GGG - chr3_1:51340979-51340998 None:intergenic 35.0%
CTCACATGATTACTATGTGA+TGG - chr3_1:51341100-51341119 None:intergenic 35.0%
ACTTTGTGGTTGAGCTAAAA+GGG - chr3_1:51341127-51341146 None:intergenic 35.0%
TACTTTGTGGTTGAGCTAAA+AGG - chr3_1:51341128-51341147 None:intergenic 35.0%
!!! ATTTTTGGTCTGCTTTTGTC+AGG + chr3_1:51341314-51341333 Msa0361130:five_prime_UTR 35.0%
TCATCTACGATCCATGAAAA+GGG - chr3_1:51341387-51341406 None:intergenic 35.0%
ATCATCTACGATCCATGAAA+AGG - chr3_1:51341388-51341407 None:intergenic 35.0%
TGGATCGTAGATGATGTATT+TGG + chr3_1:51341393-51341412 Msa0361130:intron 35.0%
!!! TGTCGGCAATTAATGGATTT+TGG - chr3_1:51341432-51341451 None:intergenic 35.0%
AAAATCCATTAATTGCCGAC+AGG + chr3_1:51341431-51341450 Msa0361130:intron 35.0%
AATTTCCTGTCGGCAATTAA+TGG - chr3_1:51341439-51341458 None:intergenic 35.0%
TGTTGTCCTTGATAAATAGC+AGG + chr3_1:51341481-51341500 Msa0361130:intron 35.0%
! TATTTTACCCCTCTGTTTTG+AGG + chr3_1:51341583-51341602 Msa0361130:five_prime_UTR 35.0%
ATATAGTCCTCAAAACAGAG+GGG - chr3_1:51341593-51341612 None:intergenic 35.0%
! AGGTTCATTCTGTGAGATTT+TGG + chr3_1:51341719-51341738 Msa0361130:five_prime_UTR 35.0%
! TTCATTCTGTGAGATTTTGG+TGG + chr3_1:51341722-51341741 Msa0361130:five_prime_UTR 35.0%
GAACCAAAATGATATCAGTG+AGG - chr3_1:51341776-51341795 None:intergenic 35.0%
GAGTTTCGACAAACAATCAA+TGG - chr3_1:51341825-51341844 None:intergenic 35.0%
GATCAGGTTTCAAAAAGGTT+GGG - chr3_1:51341886-51341905 None:intergenic 35.0%
TGATCAGGTTTCAAAAAGGT+TGG - chr3_1:51341887-51341906 None:intergenic 35.0%
TGGTTGATCAGGTTTCAAAA+AGG - chr3_1:51341891-51341910 None:intergenic 35.0%
!!! TGAACAGATTTTGGTTGATC+AGG - chr3_1:51341902-51341921 None:intergenic 35.0%
!! AGTTTTGCTGATTGATGAGA+AGG + chr3_1:51341922-51341941 Msa0361130:five_prime_UTR 35.0%
GTACCCTCTGTTATTGTTAT+AGG + chr3_1:51342013-51342032 Msa0361130:intron 35.0%
CACCCTATAACAATAACAGA+GGG - chr3_1:51342019-51342038 None:intergenic 35.0%
! GTATGTTTTCCAGAAGGATT+AGG + chr3_1:51342056-51342075 Msa0361130:five_prime_UTR 35.0%
TATTTAGGTCCTAATCCTTC+TGG - chr3_1:51342068-51342087 None:intergenic 35.0%
GAAGGATTAGGACCTAAATA+TGG + chr3_1:51342068-51342087 Msa0361130:exon 35.0%
! AAGTTTCTTGCTGTCAAAGA+AGG + chr3_1:51342143-51342162 Msa0361130:intron 35.0%
!! AAGTGCTGATCTTCTTACAA+TGG + chr3_1:51342321-51342340 Msa0361130:intron 35.0%
CCTGTCATATGTAAAGTTTG+AGG - chr3_1:51342402-51342421 None:intergenic 35.0%
CCTCAAACTTTACATATGAC+AGG + chr3_1:51342399-51342418 Msa0361130:intron 35.0%
CAAACTTTACATATGACAGG+TGG + chr3_1:51342402-51342421 Msa0361130:intron 35.0%
TTTATGCAATTCAAGAAGGC+TGG + chr3_1:51342521-51342540 Msa0361130:intron 35.0%
GTTGATCTTAATAGAGGAAC+AGG + chr3_1:51342738-51342757 Msa0361130:intron 35.0%
GAAGAAGAAAAAAGGAAGAC+AGG - chr3_1:51342774-51342793 None:intergenic 35.0%
GTTGTCTGCTTCAAAACAAA+GGG - chr3_1:51342960-51342979 None:intergenic 35.0%
GTTTGTAAAGCTCTTGAGTT+TGG - chr3_1:51342982-51343001 None:intergenic 35.0%
ACAAGATAGAAATCGAGGTT+GGG + chr3_1:51343183-51343202 Msa0361130:intron 35.0%
! TGGGGATTAGTACAAATCAA+AGG + chr3_1:51343202-51343221 Msa0361130:intron 35.0%
! GGGGATTAGTACAAATCAAA+GGG + chr3_1:51343203-51343222 Msa0361130:intron 35.0%
TGAAGTCTCTCAACACAAAT+GGG - chr3_1:51343251-51343270 None:intergenic 35.0%
ATGAAGTCTCTCAACACAAA+TGG - chr3_1:51343252-51343271 None:intergenic 35.0%
! TGTGTTGAGAGACTTCATTT+TGG + chr3_1:51343254-51343273 Msa0361130:intron 35.0%
GTAAATATTTGCATCTGGGA+TGG + chr3_1:51343600-51343619 Msa0361130:intron 35.0%
TATTGTCTGAGTTGCAGATA+AGG + chr3_1:51343674-51343693 Msa0361130:intron 35.0%
TGACATGAGTGCTATATTTC+AGG + chr3_1:51343848-51343867 Msa0361130:intron 35.0%
GACATGAGTGCTATATTTCA+GGG + chr3_1:51343849-51343868 Msa0361130:intron 35.0%
ATCAAAAGACAACCTTACAC+GGG - chr3_1:51343923-51343942 None:intergenic 35.0%
CATCAAAAGACAACCTTACA+CGG - chr3_1:51343924-51343943 None:intergenic 35.0%
GAGAGTTATGAATATGACTG+TGG + chr3_1:51344036-51344055 Msa0361130:CDS 35.0%
GTTATGAATATGACTGTGGT+TGG + chr3_1:51344040-51344059 Msa0361130:CDS 35.0%
GATTGAATTGCAGGGTAATT+TGG + chr3_1:51344069-51344088 Msa0361130:exon 35.0%
AATTGCAGGGTAATTTGGAA+TGG + chr3_1:51344074-51344093 Msa0361130:exon 35.0%
! ATTGCAGGGTAATTTGGAAT+GGG + chr3_1:51344075-51344094 Msa0361130:three_prime_UTR 35.0%
GAATCTACACACACAAGAAA+TGG - chr3_1:51344193-51344212 None:intergenic 35.0%
TAGGGCTTCTTTCTTCTCAA+AGG - chr3_1:51340939-51340958 None:intergenic 40.0%
GGATCTCTCTGCAACAAATT+AGG - chr3_1:51340958-51340977 None:intergenic 40.0%
GAGAGATCCCTGATTTAACT+TGG + chr3_1:51340969-51340988 Msa0361130:five_prime_UTR 40.0%
CTATACGGCCAAGTTAAATC+AGG - chr3_1:51340980-51340999 None:intergenic 40.0%
!! ATCTTGGTGATGGCACTATA+CGG - chr3_1:51340995-51341014 None:intergenic 40.0%
ACCATTTCAGCAGCTTACAA+TGG + chr3_1:51341072-51341091 Msa0361130:five_prime_UTR 40.0%
TCCATTGTAAGCTGCTGAAA+TGG - chr3_1:51341076-51341095 None:intergenic 40.0%
CTTTGTGGTTGAGCTAAAAG+GGG - chr3_1:51341126-51341145 None:intergenic 40.0%
TTAGGTAGTAGACAACTGGT+TGG + chr3_1:51341160-51341179 Msa0361130:five_prime_UTR 40.0%
TAGGTAGTAGACAACTGGTT+GGG + chr3_1:51341161-51341180 Msa0361130:five_prime_UTR 40.0%
ATTCATCGCAGAAATTGTCG+AGG + chr3_1:51341533-51341552 Msa0361130:five_prime_UTR 40.0%
TTCATCGCAGAAATTGTCGA+GGG + chr3_1:51341534-51341553 Msa0361130:five_prime_UTR 40.0%
AAATTGAAACCTGCTCAGCT+CGG - chr3_1:51341633-51341652 None:intergenic 40.0%
AGCAGGTTTCAATTTGTCAC+TGG + chr3_1:51341638-51341657 Msa0361130:five_prime_UTR 40.0%
CACTGGAAAAAACCTAAAGC+TGG + chr3_1:51341655-51341674 Msa0361130:five_prime_UTR 40.0%
TGTTTGTCGAAACTCTGATG+TGG + chr3_1:51341830-51341849 Msa0361130:five_prime_UTR 40.0%
GCACCCTATAACAATAACAG+AGG - chr3_1:51342020-51342039 None:intergenic 40.0%
TAATGGAGCTACTGTATTGC+AGG + chr3_1:51342119-51342138 Msa0361130:CDS 40.0%
! TTTCTTGCTGTCAAAGAAGG+TGG + chr3_1:51342146-51342165 Msa0361130:intron 40.0%
! TTCTTGCTGTCAAAGAAGGT+GGG + chr3_1:51342147-51342166 Msa0361130:intron 40.0%
! TGTCAAAGAAGGTGGGTTTA+GGG + chr3_1:51342154-51342173 Msa0361130:intron 40.0%
GTATATGCGGTACTAACTCA+AGG - chr3_1:51342913-51342932 None:intergenic 40.0%
GGTTGTCTGCTTCAAAACAA+AGG - chr3_1:51342961-51342980 None:intergenic 40.0%
CACAAGATAGAAATCGAGGT+TGG + chr3_1:51343182-51343201 Msa0361130:intron 40.0%
CAAGATAGAAATCGAGGTTG+GGG + chr3_1:51343184-51343203 Msa0361130:intron 40.0%
GATCAATCCTGACATTCTAC+AGG + chr3_1:51343734-51343753 Msa0361130:CDS 40.0%
AGGCTCTTCAACTGAAGAAT+GGG + chr3_1:51343754-51343773 Msa0361130:CDS 40.0%
ATGAGTGCTATATTTCAGGG+TGG + chr3_1:51343852-51343871 Msa0361130:intron 40.0%
TGAGTGCTATATTTCAGGGT+GGG + chr3_1:51343853-51343872 Msa0361130:intron 40.0%
TCAAAAGACAACCTTACACG+GGG - chr3_1:51343922-51343941 None:intergenic 40.0%
TATATCAGTCAGCTGAGCAA+TGG - chr3_1:51343977-51343996 None:intergenic 40.0%
TGTGTACATCTAAAGGGTGA+TGG + chr3_1:51344105-51344124 Msa0361130:three_prime_UTR 40.0%
GTGTACATCTAAAGGGTGAT+GGG + chr3_1:51344106-51344125 Msa0361130:three_prime_UTR 40.0%
!! AAAGGGTGATGGGATTATTG+TGG + chr3_1:51344116-51344135 Msa0361130:three_prime_UTR 40.0%
TGTGGTAGTATGAGTTGTTG+AGG + chr3_1:51344134-51344153 Msa0361130:three_prime_UTR 40.0%
AAGGACAATAAGTGTGCTGT+TGG - chr3_1:51344383-51344402 None:intergenic 40.0%
TGACCTGAGAATCTTGGTGA+TGG - chr3_1:51341005-51341024 None:intergenic 45.0%
!!! CAGCAGCTATTTTGGGTTCA+AGG - chr3_1:51341029-51341048 None:intergenic 45.0%
!! TGTGTGGCAGCAGCTATTTT+GGG - chr3_1:51341036-51341055 None:intergenic 45.0%
AGATGAGAACACCATCTGTG+TGG - chr3_1:51341052-51341071 None:intergenic 45.0%
TAGTAGACAACTGGTTGGGA+AGG + chr3_1:51341165-51341184 Msa0361130:five_prime_UTR 45.0%
TCATCGCAGAAATTGTCGAG+GGG + chr3_1:51341535-51341554 Msa0361130:five_prime_UTR 45.0%
TGGTTGCTGTGTTCGTTGTT+TGG + chr3_1:51341675-51341694 Msa0361130:five_prime_UTR 45.0%
CGAAACTCTGATGTGGTTCA+TGG + chr3_1:51341837-51341856 Msa0361130:five_prime_UTR 45.0%
TGGTTCATGGTAGAGAGACA+TGG + chr3_1:51341850-51341869 Msa0361130:five_prime_UTR 45.0%
TTCATGGTAGAGAGACATGG+AGG + chr3_1:51341853-51341872 Msa0361130:five_prime_UTR 45.0%
! CTGTCAAAGAAGGTGGGTTT+AGG + chr3_1:51342153-51342172 Msa0361130:intron 45.0%
! AAGAAGGTGGGTTTAGGGAT+TGG + chr3_1:51342159-51342178 Msa0361130:intron 45.0%
! AGAAGGTGGGTTTAGGGATT+GGG + chr3_1:51342160-51342179 Msa0361130:intron 45.0%
TGAAGAGCCTGTAGAATGTC+AGG - chr3_1:51343744-51343763 None:intergenic 45.0%
CAGGCTCTTCAACTGAAGAA+TGG + chr3_1:51343753-51343772 Msa0361130:CDS 45.0%
! TATTTCAGGGTGGGCTTTGA+TGG + chr3_1:51343862-51343881 Msa0361130:intron 45.0%
TTGATGGAGTTGAGACATCC+AGG + chr3_1:51343878-51343897 Msa0361130:CDS 45.0%
CTGACTGATATAGCTGACGA+TGG + chr3_1:51343985-51344004 Msa0361130:CDS 45.0%
TGAATATGACTGTGGTTGGG+TGG + chr3_1:51344044-51344063 Msa0361130:CDS 45.0%
TGGGTGGTTGATTGAATTGC+AGG + chr3_1:51344060-51344079 Msa0361130:exon 45.0%
GGGTGGTTGATTGAATTGCA+GGG + chr3_1:51344061-51344080 Msa0361130:exon 45.0%
GTGCCATCACCAAGATTCTC+AGG + chr3_1:51340999-51341018 Msa0361130:five_prime_UTR 50.0%
CAAGGCTGACCTGAGAATCT+TGG - chr3_1:51341011-51341030 None:intergenic 50.0%
! CTGTGTGGCAGCAGCTATTT+TGG - chr3_1:51341037-51341056 None:intergenic 50.0%
AATAGCTGCTGCCACACAGA+TGG + chr3_1:51341038-51341057 Msa0361130:five_prime_UTR 50.0%
! TTTCAGGTGAGGGGAAGAGT+TGG - chr3_1:51341216-51341235 None:intergenic 50.0%
CGAACACAGCAACCAGCTTT+AGG - chr3_1:51341670-51341689 None:intergenic 50.0%
TCCAGGACTGTTCCCAAACT+TGG + chr3_1:51343895-51343914 Msa0361130:CDS 50.0%
ACCAAGTTTGGGAACAGTCC+TGG - chr3_1:51343899-51343918 None:intergenic 50.0%
CTTACACGGGGACCAAGTTT+GGG - chr3_1:51343910-51343929 None:intergenic 50.0%
TCGCAGAAATTGTCGAGGGG+AGG + chr3_1:51341538-51341557 Msa0361130:five_prime_UTR 55.0%
CGCAGAAATTGTCGAGGGGA+GGG + chr3_1:51341539-51341558 Msa0361130:five_prime_UTR 55.0%
GTTTGTGATCCGAGCTGAGC+AGG + chr3_1:51341621-51341640 Msa0361130:five_prime_UTR 55.0%
CCTTACACGGGGACCAAGTT+TGG - chr3_1:51343911-51343930 None:intergenic 55.0%
CCAAACTTGGTCCCCGTGTA+AGG + chr3_1:51343908-51343927 Msa0361130:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 51340903 51344440 51340903 ID=Msa0361130;Name=Msa0361130
chr3_1 mRNA 51340903 51344440 51340903 ID=Msa0361130-mRNA-1;Parent=Msa0361130;Name=Msa0361130-mRNA-1;_AED=0.34;_eAED=0.34;_QI=926|0.8|1|1|0.6|0.66|6|366|129
chr3_1 exon 51340903 51341335 51340903 ID=Msa0361130-mRNA-1:exon:9132;Parent=Msa0361130-mRNA-1
chr3_1 exon 51341503 51341943 51341503 ID=Msa0361130-mRNA-1:exon:9133;Parent=Msa0361130-mRNA-1
chr3_1 exon 51342035 51342140 51342035 ID=Msa0361130-mRNA-1:exon:9134;Parent=Msa0361130-mRNA-1
chr3_1 exon 51342421 51342466 51342421 ID=Msa0361130-mRNA-1:exon:9135;Parent=Msa0361130-mRNA-1
chr3_1 exon 51343691 51343775 51343691 ID=Msa0361130-mRNA-1:exon:9136;Parent=Msa0361130-mRNA-1
chr3_1 exon 51343870 51344440 51343870 ID=Msa0361130-mRNA-1:exon:9137;Parent=Msa0361130-mRNA-1
chr3_1 five_prime_UTR 51340903 51341335 51340903 ID=Msa0361130-mRNA-1:five_prime_utr;Parent=Msa0361130-mRNA-1
chr3_1 five_prime_UTR 51341503 51341943 51341503 ID=Msa0361130-mRNA-1:five_prime_utr;Parent=Msa0361130-mRNA-1
chr3_1 five_prime_UTR 51342035 51342086 51342035 ID=Msa0361130-mRNA-1:five_prime_utr;Parent=Msa0361130-mRNA-1
chr3_1 CDS 51342087 51342140 51342087 ID=Msa0361130-mRNA-1:cds;Parent=Msa0361130-mRNA-1
chr3_1 CDS 51342421 51342466 51342421 ID=Msa0361130-mRNA-1:cds;Parent=Msa0361130-mRNA-1
chr3_1 CDS 51343691 51343775 51343691 ID=Msa0361130-mRNA-1:cds;Parent=Msa0361130-mRNA-1
chr3_1 CDS 51343870 51344074 51343870 ID=Msa0361130-mRNA-1:cds;Parent=Msa0361130-mRNA-1
chr3_1 three_prime_UTR 51344075 51344440 51344075 ID=Msa0361130-mRNA-1:three_prime_utr;Parent=Msa0361130-mRNA-1
Gene Sequence

>Msa0361130

ATGGAAAGTAATCTAAATACAGTATTGTATACTAATGGAGCTACTGTATTGCAGGTGGAGCAGTTCCTAAAAGCATTCAAGTTGAAGAATTTTTATTTAGATAAGGCATTTGTGCAGAATGCGATGAATATAACTAGGCTCTTGATCAATCCTGACATTCTACAGGCTCTTCAACTGAAGAATGGGGTGGGCTTTGATGGAGTTGAGACATCCAGGACTGTTCCCAAACTTGGTCCCCGTGTAAGGTTGTCTTTTGATGAAGTGTTTTTGCGATTGTATGCAAAGAAAACCATTGCTCAGCTGACTGATATAGCTGACGATGGAACTTTTATTGTAAGTGCAACTGATAATGAGAGTTATGAATATGACTGTGGTTGGGTGGTTGATTGA
Protein sequence

>Msa0361130

MESNLNTVLYTNGATVLQVEQFLKAFKLKNFYLDKAFVQNAMNITRLLINPDILQALQLKNGVGFDGVETSRTVPKLGPRVRLSFDEVFLRLYAKKTIAQLTDIADDGTFIVSATDNESYEYDCGWVVD*