AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0383030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383030 MtrunA17_Chr3g0138101 99.548 221 1 0 1 221 1 221 4.20e-168 461
Msa0383030 MtrunA17_Chr3g0138121 98.643 221 3 0 1 221 1 221 6.05e-167 458
Msa0383030 MtrunA17_Chr3g0138111 98.643 221 3 0 1 221 1 221 1.59e-166 457
Msa0383030 MtrunA17_Chr4g0063211 72.993 137 25 1 85 221 7 131 1.18e-66 201
Msa0383030 MtrunA17_Chr4g0055081 30.052 193 128 4 14 203 10 198 3.80e-27 103
Msa0383030 MtrunA17_Chr4g0046261 30.526 190 126 4 14 197 14 203 2.98e-26 100
Msa0383030 MtrunA17_Chr5g0400131 31.977 172 106 3 14 178 10 177 3.73e-26 100
Msa0383030 MtrunA17_Chr3g0109721 33.526 173 109 4 15 184 12 181 7.23e-26 99.8
Msa0383030 MtrunA17_Chr8g0390561 34.161 161 103 3 15 172 11 171 8.08e-26 99.4
Msa0383030 MtrunA17_Chr2g0283131 30.526 190 126 4 14 197 14 203 1.33e-25 99.4
Msa0383030 MtrunA17_Chr8g0383261 30.723 166 105 3 14 169 13 178 1.41e-25 99.0
Msa0383030 MtrunA17_Chr1g0201121 27.749 191 125 4 14 191 9 199 1.63e-25 99.0
Msa0383030 MtrunA17_Chr8g0349711 32.716 162 106 3 13 171 6 167 3.20e-25 98.2
Msa0383030 MtrunA17_Chr1g0159481 37.647 170 98 6 14 177 18 185 4.85e-25 97.8
Msa0383030 MtrunA17_Chr4g0032201 31.844 179 114 3 14 189 13 186 5.68e-25 97.8
Msa0383030 MtrunA17_Chr8g0349681 33.951 162 104 3 13 171 175 336 6.09e-25 102
Msa0383030 MtrunA17_Chr8g0349681 32.515 163 107 3 13 172 13 175 2.41e-22 94.7
Msa0383030 MtrunA17_Chr8g0349681 32.298 161 106 2 14 171 385 545 5.72e-22 93.6
Msa0383030 MtrunA17_Chr8g0381841 31.915 188 119 5 7 191 12 193 8.84e-25 97.4
Msa0383030 MtrunA17_Chr8g0384311 34.637 179 110 5 4 177 5 181 1.04e-24 96.7
Msa0383030 MtrunA17_Chr3g0123971 32.143 168 111 3 14 178 10 177 1.05e-24 96.7
Msa0383030 MtrunA17_Chr4g0040781 30.000 190 127 4 14 197 14 203 1.24e-24 96.7
Msa0383030 MtrunA17_Chr3g0112681 34.731 167 105 4 1 164 1 166 1.34e-24 96.7
Msa0383030 MtrunA17_Chr1g0182321 29.381 194 128 5 2 190 3 192 1.43e-24 96.7
Msa0383030 MtrunA17_Chr5g0433561 32.948 173 110 4 15 184 12 181 1.53e-24 96.3
Msa0383030 MtrunA17_Chr6g0482721 34.483 174 110 4 1 171 1 173 1.74e-24 96.3
Msa0383030 MtrunA17_Chr4g0055651 30.435 184 119 4 14 192 9 188 2.20e-24 95.9
Msa0383030 MtrunA17_Chr1g0182301 28.866 194 129 5 2 190 3 192 4.95e-24 95.1
Msa0383030 MtrunA17_Chr2g0319951 33.117 154 100 2 14 164 14 167 5.62e-24 95.1
Msa0383030 MtrunA17_Chr2g0325091 32.903 155 101 2 14 165 12 166 5.67e-24 95.1
Msa0383030 MtrunA17_Chr1g0212521 30.247 162 109 3 14 171 16 177 6.37e-24 94.7
Msa0383030 MtrunA17_Chr4g0030911 30.247 162 109 3 14 171 16 177 7.69e-24 94.7
Msa0383030 MtrunA17_Chr8g0382221 32.143 168 111 3 14 178 10 177 8.58e-24 94.4
Msa0383030 MtrunA17_Chr2g0276941 34.416 154 98 2 14 164 16 169 9.33e-24 94.7
Msa0383030 MtrunA17_Chr6g0466301 27.513 189 124 4 14 189 9 197 9.42e-24 94.4
Msa0383030 MtrunA17_Chr6g0465491 27.513 189 124 4 14 189 9 197 9.42e-24 94.4
Msa0383030 MtrunA17_Chr3g0123821 29.126 206 129 5 7 198 11 213 1.20e-23 94.4
Msa0383030 MtrunA17_Chr7g0263071 30.247 162 109 3 14 171 16 177 1.32e-23 94.0
Msa0383030 MtrunA17_Chr2g0315551 32.468 154 101 2 14 164 14 167 1.58e-23 94.0
Msa0383030 MtrunA17_Chr6g0458081 32.298 161 106 2 14 171 7 167 2.66e-23 93.2
Msa0383030 MtrunA17_Chr8g0351861 31.765 170 112 4 5 171 9 177 3.51e-23 93.2
Msa0383030 MtrunA17_Chr8g0335471 32.447 188 111 6 14 192 9 189 6.13e-23 92.0
Msa0383030 MtrunA17_Chr7g0275991 33.333 168 108 3 7 171 15 181 7.87e-23 92.4
Msa0383030 MtrunA17_Chr7g0265001 35.088 171 104 5 13 177 16 185 8.05e-23 92.0
Msa0383030 MtrunA17_Chr7g0250531 30.108 186 117 4 14 192 9 188 1.04e-22 91.3
Msa0383030 MtrunA17_Chr4g0034871 31.214 173 95 6 14 169 9 174 1.10e-22 91.3
Msa0383030 MtrunA17_Chr3g0130271 34.973 183 111 6 1 177 1 181 1.59e-22 90.9
Msa0383030 MtrunA17_Chr7g0214241 31.056 161 108 3 14 171 16 176 1.86e-22 91.3
Msa0383030 MtrunA17_Chr2g0331691 31.677 161 100 3 14 164 11 171 2.83e-22 90.5
Msa0383030 MtrunA17_Chr8g0392931 33.121 157 102 2 14 167 15 171 3.15e-22 90.5
Msa0383030 MtrunA17_Chr2g0327911 28.834 163 111 2 14 171 16 178 6.30e-22 89.7
Msa0383030 MtrunA17_Chr8g0346421 33.136 169 109 4 6 171 20 187 8.69e-22 89.7
Msa0383030 MtrunA17_Chr4g0004661 31.792 173 94 6 14 169 65 230 9.01e-22 90.1
Msa0383030 MtrunA17_Chr1g0180291 32.749 171 107 6 13 177 14 182 1.11e-21 89.0
Msa0383030 MtrunA17_Chr1g0174281 31.098 164 109 3 7 167 10 172 2.34e-21 88.2
Msa0383030 MtrunA17_Chr7g0231131 32.716 162 105 4 9 167 12 172 2.72e-21 88.2
Msa0383030 MtrunA17_Chr2g0290891 31.646 158 105 2 14 168 7 164 3.13e-21 87.8
Msa0383030 MtrunA17_Chr1g0189361 30.114 176 117 5 1 171 1 175 1.19e-20 86.3
Msa0383030 MtrunA17_Chr1g0199331 29.193 161 110 3 15 171 33 193 4.62e-20 84.3
Msa0383030 MtrunA17_Chr7g0234201 28.571 161 112 3 14 171 14 174 4.73e-20 84.7
Msa0383030 MtrunA17_Chr2g0276931 30.061 163 107 4 14 171 10 170 1.07e-19 83.6
Msa0383030 MtrunA17_Chr1g0197331 29.442 197 123 7 10 199 13 200 7.80e-19 81.3
Msa0383030 MtrunA17_Chr8g0371501 28.485 165 103 4 14 164 7 170 3.92e-14 68.6
Msa0383030 MtrunA17_Chr6g0486041 33.051 118 77 2 14 130 7 123 5.34e-14 68.2
Msa0383030 MtrunA17_Chr5g0405791 28.485 165 103 4 14 164 7 170 9.04e-14 67.4
Msa0383030 MtrunA17_Chr4g0046211 30.252 119 81 2 14 131 7 124 9.33e-14 66.2
Msa0383030 MtrunA17_Chr6g0466161 27.815 151 99 3 29 169 6 156 1.60e-13 67.0
Msa0383030 MtrunA17_Chr6g0465471 27.815 151 99 3 29 169 6 156 1.98e-13 66.6
Msa0383030 MtrunA17_Chr5g0443631 26.012 173 121 1 10 175 101 273 1.53e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383030 AT5G55190.1 96.833 221 7 0 1 221 1 221 7.36e-164 451
Msa0383030 AT5G20010.1 95.475 221 10 0 1 221 1 221 4.71e-161 444
Msa0383030 AT5G20020.1 95.023 221 11 0 1 221 1 221 1.41e-152 422
Msa0383030 AT5G55080.1 70.283 212 58 1 1 212 1 207 1.37e-108 311
Msa0383030 AT4G39890.1 36.158 177 103 4 14 186 10 180 4.84e-28 105
Msa0383030 AT1G07410.1 31.551 187 120 3 14 197 13 194 4.45e-26 100
Msa0383030 AT4G19640.1 33.514 185 116 5 15 195 12 193 5.22e-26 100
Msa0383030 AT2G44610.1 32.143 168 111 3 14 178 10 177 9.85e-26 99.8
Msa0383030 AT4G18430.1 32.298 161 106 3 14 171 14 174 2.46e-25 99.0
Msa0383030 AT5G45130.1 33.140 172 109 4 15 183 12 180 2.54e-25 98.6
Msa0383030 AT5G39620.1 32.335 167 103 3 14 170 6 172 3.30e-25 98.2
Msa0383030 AT5G59150.1 34.731 167 105 3 1 164 1 166 7.86e-25 97.8
Msa0383030 AT5G45750.1 35.256 156 94 3 14 164 14 167 9.91e-25 97.4
Msa0383030 AT4G18800.1 32.597 181 110 4 14 189 14 187 1.54e-24 96.7
Msa0383030 AT1G09630.1 34.132 167 106 3 1 164 1 166 1.66e-24 96.7
Msa0383030 AT2G21880.1 30.909 165 104 3 14 168 10 174 1.94e-24 96.7
Msa0383030 AT5G65270.1 31.217 189 121 5 6 191 11 193 2.87e-24 96.3
Msa0383030 AT5G60860.1 30.000 190 127 4 14 197 14 203 3.17e-24 96.3
Msa0383030 AT2G22290.1 32.515 163 107 3 14 173 10 172 3.48e-24 95.9
Msa0383030 AT1G18200.1 32.919 161 105 3 14 171 14 174 3.50e-24 96.3
Msa0383030 AT4G09720.4 29.240 171 106 3 14 169 9 179 3.89e-24 95.9
Msa0383030 AT4G39990.1 33.333 168 104 4 9 171 14 178 4.70e-24 95.9
Msa0383030 AT3G15060.1 33.766 154 99 3 14 164 14 167 4.73e-24 95.5
Msa0383030 AT4G09720.1 28.916 166 108 3 14 169 9 174 5.19e-24 95.1
Msa0383030 AT3G18820.1 30.882 204 113 7 14 200 9 201 6.21e-24 95.1
Msa0383030 AT1G22740.1 29.630 189 120 6 14 189 9 197 6.38e-24 95.1
Msa0383030 AT3G53610.2 28.729 181 121 4 14 190 16 192 6.76e-24 95.1
Msa0383030 AT3G53610.3 28.729 181 121 4 14 190 16 192 6.76e-24 95.1
Msa0383030 AT3G53610.1 28.729 181 121 4 14 190 16 192 6.76e-24 95.1
Msa0383030 AT5G59840.1 29.319 191 123 5 14 196 16 202 6.83e-24 95.1
Msa0383030 AT5G10260.1 32.738 168 110 3 14 178 10 177 1.33e-23 94.4
Msa0383030 AT3G46830.1 34.783 161 101 3 7 164 7 166 1.57e-23 94.4
Msa0383030 AT3G46830.2 34.783 161 101 3 7 164 7 166 1.57e-23 94.4
Msa0383030 AT5G45130.2 32.353 170 109 4 17 183 22 188 1.68e-23 94.0
Msa0383030 AT3G46060.2 30.247 162 109 3 14 171 16 177 2.42e-23 93.6
Msa0383030 AT3G46060.3 30.247 162 109 3 14 171 16 177 2.42e-23 93.6
Msa0383030 AT3G46060.1 30.247 162 109 3 14 171 16 177 2.42e-23 93.6
Msa0383030 AT4G09720.3 28.249 177 106 3 14 169 9 185 2.42e-23 94.0
Msa0383030 AT4G17530.1 30.435 184 119 4 14 192 9 188 5.32e-23 92.4
Msa0383030 AT5G03530.2 32.353 170 109 4 13 177 13 181 6.62e-23 92.4
Msa0383030 AT5G03530.1 32.353 170 109 4 13 177 13 181 6.62e-23 92.4
Msa0383030 AT5G47200.1 30.435 184 119 4 14 192 9 188 7.45e-23 92.0
Msa0383030 AT1G73640.1 32.544 169 110 4 6 171 7 174 9.24e-23 92.8
Msa0383030 AT3G12160.1 32.927 164 106 4 7 167 10 172 1.01e-22 92.4
Msa0383030 AT5G47960.1 33.951 162 103 4 7 165 10 170 1.02e-22 92.4
Msa0383030 AT5G64990.1 28.079 203 124 6 14 203 8 201 1.63e-22 91.3
Msa0383030 AT2G33870.1 30.270 185 123 4 14 192 14 198 1.94e-22 91.3
Msa0383030 AT3G16100.1 29.798 198 123 5 14 200 9 201 2.14e-22 90.9
Msa0383030 AT1G28550.1 29.730 185 124 4 14 192 14 198 2.34e-22 91.3
Msa0383030 AT1G06400.1 33.766 154 99 3 14 164 14 167 2.47e-22 91.3
Msa0383030 AT1G02130.1 30.811 185 118 5 14 192 9 189 3.26e-22 90.5
Msa0383030 AT1G49300.2 31.977 172 93 6 14 168 9 173 3.44e-22 90.5
Msa0383030 AT1G49300.1 31.977 172 93 6 14 168 9 173 3.44e-22 90.5
Msa0383030 AT1G52280.1 29.293 198 124 6 14 200 9 201 4.25e-22 90.1
Msa0383030 AT1G43890.3 33.136 169 107 4 14 177 14 181 4.30e-22 90.5
Msa0383030 AT1G43890.1 33.136 169 107 4 14 177 14 181 4.30e-22 90.5
Msa0383030 AT1G43890.2 33.136 169 107 4 14 177 14 181 4.30e-22 90.5
Msa0383030 AT1G16920.1 31.818 154 102 2 14 164 14 167 4.88e-22 90.5
Msa0383030 AT2G43130.1 27.461 193 136 4 14 203 13 204 7.20e-22 89.7
Msa0383030 AT4G35860.1 31.056 161 108 2 14 171 7 167 7.75e-22 89.7
Msa0383030 AT1G01200.1 31.953 169 110 4 7 171 23 190 1.03e-21 90.1
Msa0383030 AT4G17160.1 30.380 158 107 2 14 168 7 164 3.19e-21 87.8
Msa0383030 AT4G17170.1 31.056 161 108 2 14 171 7 167 6.37e-21 87.4
Msa0383030 AT3G07410.1 29.310 174 120 3 14 184 13 186 1.19e-20 86.7
Msa0383030 AT1G05810.1 30.519 154 104 3 14 164 13 166 1.35e-20 86.7
Msa0383030 AT5G47520.1 30.435 161 109 3 14 171 15 175 1.46e-20 86.7
Msa0383030 AT1G05810.2 30.519 154 104 3 14 164 59 212 3.90e-20 86.3
Msa0383030 AT4G19640.2 35.938 128 80 2 15 140 12 139 5.46e-20 83.2
Msa0383030 AT4G19640.3 34.043 141 90 3 15 152 12 152 6.85e-20 84.0
Msa0383030 AT5G64990.2 27.143 210 124 7 14 203 8 208 8.94e-20 84.3
Msa0383030 AT3G54840.1 29.814 161 109 4 15 171 35 195 9.68e-20 84.0
Msa0383030 AT5G03520.1 28.272 191 128 5 4 189 5 191 1.70e-19 83.6
Msa0383030 AT2G31680.1 29.193 161 111 3 14 171 13 173 1.73e-19 83.6
Msa0383030 AT3G11730.1 28.108 185 126 3 14 191 9 193 2.86e-19 82.8
Msa0383030 AT3G54840.3 29.375 160 109 4 16 171 2 161 3.64e-19 81.6
Msa0383030 AT3G09900.1 28.272 191 128 5 4 189 5 191 3.77e-19 82.8
Msa0383030 AT3G54840.2 30.519 154 103 4 15 164 35 188 1.64e-18 80.5
Msa0383030 AT3G09910.3 31.176 170 111 4 13 177 13 181 1.93e-17 77.8
Msa0383030 AT3G09910.1 31.176 170 111 4 13 177 13 181 1.93e-17 77.8
Msa0383030 AT3G09910.2 31.176 170 111 4 13 177 13 181 1.93e-17 77.8
Msa0383030 AT2G21880.4 29.530 149 95 3 30 168 1 149 2.68e-17 77.0
Msa0383030 AT2G21880.3 29.530 149 95 3 30 168 1 149 2.68e-17 77.0
Msa0383030 AT3G09910.5 31.098 164 107 4 13 171 13 175 1.00e-16 75.9
Msa0383030 AT3G09910.4 31.098 164 107 4 13 171 13 175 1.00e-16 75.9
Msa0383030 AT4G09720.6 28.777 139 89 3 41 169 2 140 2.00e-16 74.3
Msa0383030 AT4G09720.5 28.777 139 89 3 41 169 2 140 2.00e-16 74.3
Msa0383030 AT4G09720.2 28.777 139 89 3 41 169 2 140 2.00e-16 74.3
Msa0383030 AT2G21880.2 29.091 165 99 5 14 168 10 166 3.70e-16 74.3
Msa0383030 AT4G35860.2 31.858 113 74 2 62 171 9 121 6.07e-15 70.1
Msa0383030 AT5G03520.2 29.323 133 86 4 61 189 53 181 6.72e-15 70.9
Msa0383030 AT5G59150.2 33.962 106 67 2 62 164 10 115 5.76e-14 67.8
Msa0383030 AT4G35950.1 29.697 165 101 4 14 164 7 170 6.57e-14 68.2
Msa0383030 AT2G17800.2 31.933 119 79 2 14 131 7 124 9.19e-14 67.8
Msa0383030 AT2G17800.1 31.933 119 79 2 14 131 7 124 9.19e-14 67.8
Msa0383030 AT3G51300.1 32.203 118 78 2 15 131 8 124 1.09e-13 67.4
Msa0383030 AT4G28950.1 28.834 163 103 4 14 164 7 168 1.38e-13 67.4
Msa0383030 AT4G35020.4 30.702 114 77 2 15 127 8 120 7.77e-11 58.5
Msa0383030 AT4G35020.5 30.702 114 77 2 15 127 8 120 7.77e-11 58.5

Find 90 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGTAAACCCTCACTTAA+TGG 0.159303 3_1:+85806147 Msa0383030:three_prime_UTR
GAGAAGTGAATAAACGAATT+AGG 0.189362 3_1:-85803470 None:intergenic
TCCTCTTCATTTAAATGTTT+GGG 0.190717 3_1:+85806636 Msa0383030:three_prime_UTR
ATCCTCTTCATTTAAATGTT+TGG 0.215379 3_1:+85806635 Msa0383030:three_prime_UTR
GCCAAATCAATTTGAACTTC+TGG 0.258290 3_1:-85805325 None:intergenic
TTGCAATCTGTCTCTTCAAT+TGG 0.267988 3_1:-85803375 None:intergenic
GATTCTACAAAGTGCAAGTT+AGG 0.300639 3_1:-85805286 None:intergenic
GAGGATATTAACTCAATAAA+AGG 0.304458 3_1:-85806618 None:intergenic
AGAAGTGAATAAACGAATTA+GGG 0.313376 3_1:-85803469 None:intergenic
ACCAGAAGTTCAAATTGATT+TGG 0.314372 3_1:+85805324 Msa0383030:CDS
TTTGTGAAGAGGCATCTTAC+TGG 0.332899 3_1:+85803831 Msa0383030:CDS
TAATCAACGGTTTGCTGATT+GGG 0.341509 3_1:-85803634 None:intergenic
TACCTTGCTAGGAAGCTTGC+AGG 0.343483 3_1:+85805161 Msa0383030:CDS
TGCAATCTGTCTCTTCAATT+GGG 0.359194 3_1:-85803374 None:intergenic
GACGCGGCTCTTCTGTCTTT+CGG 0.366086 3_1:-85803404 None:intergenic
GCAAGATTCGCTTCTACTGC+TGG 0.380027 3_1:+85804038 Msa0383030:CDS
CACATAACTAAGTCTGTGAT+TGG 0.400841 3_1:-85806352 None:intergenic
TCTTCTTTCGAACCACACTA+TGG 0.402104 3_1:+85803517 Msa0383030:exon
TTGTTACCGCAAAGAACAAT+TGG 0.403385 3_1:-85805020 None:intergenic
AACATCCCAATTGTTCTTTG+CGG 0.404004 3_1:+85805014 Msa0383030:CDS
GTAATCAACGGTTTGCTGAT+TGG 0.420523 3_1:-85803635 None:intergenic
GAAGGGGTTGATTGGCAGCT+TGG 0.435439 3_1:-85805947 None:intergenic
CCTGGCACCGTGATCTTTGT+CGG 0.450109 3_1:+85804872 Msa0383030:CDS
GTCATCAGGAAGGGGTTGAT+TGG 0.454313 3_1:-85805955 None:intergenic
CTTTGTAGAATCTCCTGCAT+TGG 0.466850 3_1:+85805297 Msa0383030:CDS
CTAGTAAAATGTGTTAGAAC+AGG 0.467804 3_1:-85806251 None:intergenic
CAGGCAAGTGAAGGCAAAGC+AGG 0.468849 3_1:+85805058 Msa0383030:CDS
TAACTCAATAAAAGGTTGGC+TGG 0.470306 3_1:-85806610 None:intergenic
ACTGCTGGACAAGAGAAGTT+TGG 0.471338 3_1:+85804064 Msa0383030:CDS
ACAAGGTTGATGTGAAGAAC+AGG 0.475162 3_1:+85805039 Msa0383030:CDS
CGATGACAAGCTTGAAGCTC+GGG 0.476801 3_1:-85803657 None:intergenic
TGCTATCCTTTGTCTCGAGT+TGG 0.480872 3_1:+85806011 Msa0383030:three_prime_UTR
CCCCAAACATTTAAATGAAG+AGG 0.485979 3_1:-85806637 None:intergenic
ATCGTCGGCGATGGCGGCAC+TGG 0.486311 3_1:+85803676 Msa0383030:CDS
CTTGTCATCGTCGGCGATGG+CGG 0.488335 3_1:+85803670 Msa0383030:CDS
AATGCATCATCATCGTCATC+AGG 0.495924 3_1:-85805969 None:intergenic
TGATTGTAAACAGCTACAAT+TGG 0.508837 3_1:+85803977 Msa0383030:intron
CAAGCTTCCTAGCAAGGTAA+AGG 0.519388 3_1:-85805157 None:intergenic
CTTTATATTGCAGTATCCAC+GGG 0.523043 3_1:+85804791 Msa0383030:intron
CCGACAAAGATCACGGTGCC+AGG 0.524098 3_1:-85804872 None:intergenic
AATAAAAGGTTGGCTGGAGA+AGG 0.527890 3_1:-85806604 None:intergenic
AAGCTTGTCATCGTCGGCGA+TGG 0.529447 3_1:+85803667 Msa0383030:CDS
TCTTTATATTGCAGTATCCA+CGG 0.531789 3_1:+85804790 Msa0383030:intron
CCTCTTCATTTAAATGTTTG+GGG 0.532483 3_1:+85806637 Msa0383030:three_prime_UTR
TTGAAAGTATTGAAGTTGAG+AGG 0.533683 3_1:+85806428 Msa0383030:three_prime_UTR
ACTTCTGGTGGAGCCAATGC+AGG 0.537856 3_1:-85805310 None:intergenic
AGCTTCAAGCTTGTCATCGT+CGG 0.540576 3_1:+85803661 Msa0383030:CDS
AAGTTTGGTGGTCTCAGAGA+TGG 0.543632 3_1:+85804079 Msa0383030:CDS
TTCTACTGCTGGGATACTGC+TGG 0.543775 3_1:+85804049 Msa0383030:CDS
ATATTAACTCAATAAAAGGT+TGG 0.545222 3_1:-85806614 None:intergenic
AATTGTTCTTTGCGGTAACA+AGG 0.546634 3_1:+85805022 Msa0383030:CDS
TTGAAGATTAATGAGATGAA+AGG 0.546678 3_1:-85806514 None:intergenic
CAAAGCAGGTTACTTTCCAC+AGG 0.547056 3_1:+85805072 Msa0383030:CDS
CATCATCATCGTCATCAGGA+AGG 0.549641 3_1:-85805965 None:intergenic
TGTTACCGCAAAGAACAATT+GGG 0.552031 3_1:-85805019 None:intergenic
ATCATCATCGTCATCAGGAA+GGG 0.552321 3_1:-85805964 None:intergenic
AGCCATTCCTTTACCTTGCT+AGG 0.553251 3_1:+85805150 Msa0383030:CDS
AACATTTGTCGTCAAAATTG+CGG 0.560434 3_1:-85806539 None:intergenic
AAATCAATTTGAACTTCTGG+TGG 0.563330 3_1:-85805322 None:intergenic
TTGTAAACAACTTGCAGTTG+CGG 0.564300 3_1:+85806460 Msa0383030:three_prime_UTR
CAAGATTCGCTTCTACTGCT+GGG 0.564996 3_1:+85804039 Msa0383030:CDS
TTGATTTGGCTGCACAACAA+AGG 0.579779 3_1:+85805338 Msa0383030:CDS
TTGATACGTCCATTAAGTGA+GGG 0.582936 3_1:-85806156 None:intergenic
GACAAAGGATAGCAGTCGCA+CGG 0.584056 3_1:-85806002 None:intergenic
ACGTATCAATACTCTGCCGC+AGG 0.584999 3_1:+85806170 Msa0383030:three_prime_UTR
CTTGAAGCTCGGGTAATCAA+CGG 0.586019 3_1:-85803647 None:intergenic
ACCCTGCAAGCTTCCTAGCA+AGG 0.586472 3_1:-85805163 None:intergenic
TTGGATTTCTTCACAAACTG+CGG 0.590990 3_1:+85804016 Msa0383030:CDS
ACGATGACAAGCTTGAAGCT+CGG 0.595291 3_1:-85803658 None:intergenic
GAAAGACAACATTTGTGAAG+AGG 0.596908 3_1:+85803820 Msa0383030:CDS
ATTCTACAAAGTGCAAGTTA+GGG 0.598867 3_1:-85805285 None:intergenic
ATTGATACGTCCATTAAGTG+AGG 0.602666 3_1:-85806157 None:intergenic
TGGTGTGGAAGTGCATCCAT+TGG 0.604734 3_1:+85803997 Msa0383030:CDS
TTCCTAGCAAGGTAAAGGAA+TGG 0.606313 3_1:-85805152 None:intergenic
CAAAGATCACGGTGCCAGGT+AGG 0.607872 3_1:-85804868 None:intergenic
ACAAAGGATAGCAGTCGCAC+GGG 0.609636 3_1:-85806001 None:intergenic
CAGTTTGTGAAGAAATCCAA+TGG 0.610326 3_1:-85804013 None:intergenic
ACCTTGCTAGGAAGCTTGCA+GGG 0.610380 3_1:+85805162 Msa0383030:CDS
TGTGAAGAACAGGCAAGTGA+AGG 0.615131 3_1:+85805049 Msa0383030:CDS
GCTGGACAAGAGAAGTTTGG+TGG 0.616663 3_1:+85804067 Msa0383030:CDS
TCATCATCGTCATCAGGAAG+GGG 0.618873 3_1:-85805963 None:intergenic
TGAAAGTTATTAAGCCTGAA+CGG 0.622729 3_1:-85806386 None:intergenic
ACAGCACCAACTCGAGACAA+AGG 0.634897 3_1:-85806017 None:intergenic
TTATAATAGCACATTGCCCG+TGG 0.636385 3_1:-85804807 None:intergenic
CGATGATGATGCATTTGAGT+AGG 0.644342 3_1:+85805977 Msa0383030:CDS
GTAAACAGCTACAATTGGTG+TGG 0.644898 3_1:+85803982 Msa0383030:intron
TTACTAACCGACAAAGATCA+CGG 0.655820 3_1:-85804879 None:intergenic
ACAAACAAAATCAATGCCTG+CGG 0.692018 3_1:-85806186 None:intergenic
ACTGAGAGCATACCATAGTG+TGG 0.696735 3_1:-85803529 None:intergenic
TTGAAAGCGTGAAACTGACG+CGG 0.697502 3_1:-85803420 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAAGATTCATTTTAATTTA+GGG + chr3_1:85803877-85803896 Msa0383030:intron 15.0%
!! AAAAAAAAAGTCTACATTTA+TGG - chr3_1:85804503-85804522 None:intergenic 15.0%
!!! TTTTTTTTTCGTGTTAATAA+GGG + chr3_1:85804514-85804533 Msa0383030:intron 15.0%
!!! ATTTTGAATTTCTCATTTAA+TGG + chr3_1:85805895-85805914 Msa0383030:intron 15.0%
!!! TTAGGGTTTTTATATTACAA+GGG - chr3_1:85803455-85803474 None:intergenic 20.0%
!!! ATTAGGGTTTTTATATTACA+AGG - chr3_1:85803456-85803475 None:intergenic 20.0%
!!! AATTGTTATTGTTAATCGAA+CGG - chr3_1:85803587-85803606 None:intergenic 20.0%
!! ATTCTATGTTACATATTTCA+CGG - chr3_1:85803724-85803743 None:intergenic 20.0%
!!! AATAATGATCATTTTCTTAC+AGG + chr3_1:85803798-85803817 Msa0383030:intron 20.0%
!!! CGTAAGATTCATTTTAATTT+AGG + chr3_1:85803876-85803895 Msa0383030:intron 20.0%
!! TTAACAAGCATGATTATATA+CGG + chr3_1:85803900-85803919 Msa0383030:intron 20.0%
!!! CTTTTTTTTTCGTGTTAATA+AGG + chr3_1:85804513-85804532 Msa0383030:intron 20.0%
!!! CAAACAAAGTGTTAAAAAAA+AGG - chr3_1:85804920-85804939 None:intergenic 20.0%
!!! CTTTTTTTTAACACTTTGTT+TGG + chr3_1:85804918-85804937 Msa0383030:intron 20.0%
!! ATTGTAGGATAAAGAATAAA+AGG - chr3_1:85805384-85805403 None:intergenic 20.0%
!! GCTAATATACAAACTTATTA+TGG + chr3_1:85805562-85805581 Msa0383030:intron 20.0%
!! AGAGATTGAAAATAAATTTC+TGG + chr3_1:85805654-85805673 Msa0383030:intron 20.0%
!! GAGATTGAAAATAAATTTCT+GGG + chr3_1:85805655-85805674 Msa0383030:intron 20.0%
!! AGATTGAAAATAAATTTCTG+GGG + chr3_1:85805656-85805675 Msa0383030:intron 20.0%
!! TATCATTAGGTATACATTAT+TGG + chr3_1:85805682-85805701 Msa0383030:intron 20.0%
!!! AAAAAAACTAATGGATGTTA+TGG - chr3_1:85805814-85805833 None:intergenic 20.0%
!!! TAACATCCATTAGTTTTTTT+AGG + chr3_1:85805814-85805833 Msa0383030:intron 20.0%
!! GATATTCCTAAAAAAACTAA+TGG - chr3_1:85805823-85805842 None:intergenic 20.0%
!!! AATTTTATCATCATGAGTTT+GGG + chr3_1:85806113-85806132 Msa0383030:three_prime_UTR 20.0%
!!! TAAACTTTTTCAAGTGAAAT+GGG - chr3_1:85806231-85806250 None:intergenic 20.0%
!!! TCTGATTTTTTTTCTTTCTT+CGG - chr3_1:85806493-85806512 None:intergenic 20.0%
!! ATATTAACTCAATAAAAGGT+TGG - chr3_1:85806617-85806636 None:intergenic 20.0%
! AGAAGTGAATAAACGAATTA+GGG - chr3_1:85803472-85803491 None:intergenic 25.0%
! CGGAAATTAACTAAAAAGAT+TGG - chr3_1:85803704-85803723 None:intergenic 25.0%
! ATGCATTTAAATAAATGACG+AGG - chr3_1:85804119-85804138 None:intergenic 25.0%
! TAAATTCATAGTTGAAGTGA+AGG - chr3_1:85804380-85804399 None:intergenic 25.0%
! GTAATATTGGCACAAAAAAA+AGG - chr3_1:85804430-85804449 None:intergenic 25.0%
! TCATTATCATAATATTGTGC+TGG + chr3_1:85804477-85804496 Msa0383030:intron 25.0%
! CAAAATACTGATAAATGCAA+GGG - chr3_1:85804562-85804581 None:intergenic 25.0%
!! CTTGCATTTATCAGTATTTT+GGG + chr3_1:85804561-85804580 Msa0383030:intron 25.0%
!!! TGTCTTTTTGATGACATAAT+AGG + chr3_1:85804707-85804726 Msa0383030:intron 25.0%
! TACTGCAATATAAAGATAAC+AGG - chr3_1:85804786-85804805 None:intergenic 25.0%
!! AACATTTTTGTATGTTAGAC+GGG - chr3_1:85804848-85804867 None:intergenic 25.0%
!! GAACATTTTTGTATGTTAGA+CGG - chr3_1:85804849-85804868 None:intergenic 25.0%
! GTGTTAAAAAAAAGGTTTCA+AGG - chr3_1:85804912-85804931 None:intergenic 25.0%
! ATCCTACAATCATTCATTAT+AGG + chr3_1:85805394-85805413 Msa0383030:intron 25.0%
! TAATATGTTCATTGTAGCAT+TGG + chr3_1:85805422-85805441 Msa0383030:intron 25.0%
!! GATTGAAAATAAATTTCTGG+GGG + chr3_1:85805657-85805676 Msa0383030:intron 25.0%
!! GAATTTTATCATCATGAGTT+TGG + chr3_1:85806112-85806131 Msa0383030:three_prime_UTR 25.0%
!!! GTAAACTTTTTCAAGTGAAA+TGG - chr3_1:85806232-85806251 None:intergenic 25.0%
! TTGAAGATTAATGAGATGAA+AGG - chr3_1:85806517-85806536 None:intergenic 25.0%
!!! ATTTTTCAGTATGTTTGTCA+AGG - chr3_1:85806577-85806596 None:intergenic 25.0%
! GAGGATATTAACTCAATAAA+AGG - chr3_1:85806621-85806640 None:intergenic 25.0%
GAGAAGTGAATAAACGAATT+AGG - chr3_1:85803473-85803492 None:intergenic 30.0%
!! ATTGATTTGGAAGTGAACTT+CGG + chr3_1:85803768-85803787 Msa0383030:intron 30.0%
TGATTGTAAACAGCTACAAT+TGG + chr3_1:85803977-85803996 Msa0383030:intron 30.0%
!! TGTGTTCTCAAGTTTTTAAG+TGG + chr3_1:85804257-85804276 Msa0383030:intron 30.0%
! CATGGATCATTTGATTTAAC+AGG - chr3_1:85804292-85804311 None:intergenic 30.0%
GTTAAATCAAATGATCCATG+TGG + chr3_1:85804292-85804311 Msa0383030:intron 30.0%
TAAGTGATATACAAACCACA+TGG - chr3_1:85804310-85804329 None:intergenic 30.0%
!!! TTTTTTGTGCCAATATTACG+TGG + chr3_1:85804431-85804450 Msa0383030:intron 30.0%
!!! TTTTTTCGTGTTAATAAGGG+TGG + chr3_1:85804517-85804536 Msa0383030:intron 30.0%
CCAAAATACTGATAAATGCA+AGG - chr3_1:85804563-85804582 None:intergenic 30.0%
! CCTTGCATTTATCAGTATTT+TGG + chr3_1:85804560-85804579 Msa0383030:intron 30.0%
TGTAGTGGTTATATACATGT+CGG + chr3_1:85804608-85804627 Msa0383030:intron 30.0%
AGCAAGTAAAACAAGAACAA+AGG - chr3_1:85804662-85804681 None:intergenic 30.0%
!! CGTAGTCATTTTATATGTGT+CGG + chr3_1:85804684-85804703 Msa0383030:intron 30.0%
TCTTTATATTGCAGTATCCA+CGG + chr3_1:85804790-85804809 Msa0383030:intron 30.0%
ATAAAGCAACATAAGGAACA+TGG - chr3_1:85804954-85804973 None:intergenic 30.0%
!! ATGTTCCTTATGTTGCTTTA+TGG + chr3_1:85804953-85804972 Msa0383030:intron 30.0%
!! CTATTTGGAACTGATTTTCA+GGG + chr3_1:85804983-85805002 Msa0383030:intron 30.0%
ATTCTACAAAGTGCAAGTTA+GGG - chr3_1:85805288-85805307 None:intergenic 30.0%
AAATCAATTTGAACTTCTGG+TGG - chr3_1:85805325-85805344 None:intergenic 30.0%
ACCAGAAGTTCAAATTGATT+TGG + chr3_1:85805324-85805343 Msa0383030:CDS 30.0%
TGCCTATAATGAATGATTGT+AGG - chr3_1:85805399-85805418 None:intergenic 30.0%
! GCTATAATATTTTGTGCTTG+AGG + chr3_1:85806050-85806069 Msa0383030:three_prime_UTR 30.0%
! GGTTGAACTTTTAGCATTTT+TGG + chr3_1:85806071-85806090 Msa0383030:three_prime_UTR 30.0%
!!! AGCATTTTTGGTTACAGATT+TGG + chr3_1:85806083-85806102 Msa0383030:three_prime_UTR 30.0%
!!! GCATTTTTGGTTACAGATTT+GGG + chr3_1:85806084-85806103 Msa0383030:three_prime_UTR 30.0%
TTATGTAAACCCTCACTTAA+TGG + chr3_1:85806147-85806166 Msa0383030:three_prime_UTR 30.0%
CTAGTAAAATGTGTTAGAAC+AGG - chr3_1:85806254-85806273 None:intergenic 30.0%
TGAAAGTTATTAAGCCTGAA+CGG - chr3_1:85806389-85806408 None:intergenic 30.0%
!! TTGAAAGTATTGAAGTTGAG+AGG + chr3_1:85806428-85806447 Msa0383030:three_prime_UTR 30.0%
AACATTTGTCGTCAAAATTG+CGG - chr3_1:85806542-85806561 None:intergenic 30.0%
TGCAATCTGTCTCTTCAATT+GGG - chr3_1:85803377-85803396 None:intergenic 35.0%
TTGCAATCTGTCTCTTCAAT+TGG - chr3_1:85803378-85803397 None:intergenic 35.0%
CAAAAGGAATAGCGTGAAAT+CGG - chr3_1:85803564-85803583 None:intergenic 35.0%
ATTGTTAATCGAACGGCAAA+AGG - chr3_1:85803580-85803599 None:intergenic 35.0%
!! TAATCAACGGTTTGCTGATT+GGG - chr3_1:85803637-85803656 None:intergenic 35.0%
CATAGAATCAGTGATTCGTT+AGG + chr3_1:85803736-85803755 Msa0383030:intron 35.0%
! TAGGATCTGGCATATTGATT+TGG + chr3_1:85803755-85803774 Msa0383030:intron 35.0%
GAAAGACAACATTTGTGAAG+AGG + chr3_1:85803820-85803839 Msa0383030:CDS 35.0%
CAGTTTGTGAAGAAATCCAA+TGG - chr3_1:85804016-85804035 None:intergenic 35.0%
TTGGATTTCTTCACAAACTG+CGG + chr3_1:85804016-85804035 Msa0383030:CDS 35.0%
!!! CTGTGATCTCTAGTTTTTGA+TGG + chr3_1:85804139-85804158 Msa0383030:intron 35.0%
AACAGGGAATTTGGTTAAAC+AGG - chr3_1:85804168-85804187 None:intergenic 35.0%
GTTTAACCAAATTCCCTGTT+CGG + chr3_1:85804168-85804187 Msa0383030:intron 35.0%
AGTGTTACATAAACCGAACA+GGG - chr3_1:85804184-85804203 None:intergenic 35.0%
AAGTGTTACATAAACCGAAC+AGG - chr3_1:85804185-85804204 None:intergenic 35.0%
!! TCAATTGCTTGCTTTTGTAG+TGG + chr3_1:85804593-85804612 Msa0383030:intron 35.0%
GACATAATAGGATTCTCCTT+CGG + chr3_1:85804719-85804738 Msa0383030:intron 35.0%
CTTTATATTGCAGTATCCAC+GGG + chr3_1:85804791-85804810 Msa0383030:intron 35.0%
CATACAAAAATGTTCCTACC+TGG + chr3_1:85804854-85804873 Msa0383030:CDS 35.0%
TTACTAACCGACAAAGATCA+CGG - chr3_1:85804882-85804901 None:intergenic 35.0%
AGTGTCCATAAAGCAACATA+AGG - chr3_1:85804961-85804980 None:intergenic 35.0%
CTTTATGGACACTTCCTATT+TGG + chr3_1:85804968-85804987 Msa0383030:intron 35.0%
CCTGAAAATCAGTTCCAAAT+AGG - chr3_1:85804985-85805004 None:intergenic 35.0%
!! CCTATTTGGAACTGATTTTC+AGG + chr3_1:85804982-85805001 Msa0383030:intron 35.0%
AACATCCCAATTGTTCTTTG+CGG + chr3_1:85805014-85805033 Msa0383030:CDS 35.0%
TGTTACCGCAAAGAACAATT+GGG - chr3_1:85805022-85805041 None:intergenic 35.0%
TTGTTACCGCAAAGAACAAT+TGG - chr3_1:85805023-85805042 None:intergenic 35.0%
AATTGTTCTTTGCGGTAACA+AGG + chr3_1:85805022-85805041 Msa0383030:CDS 35.0%
! ACTGCAAATTTTTCTTCCTG+TGG - chr3_1:85805091-85805110 None:intergenic 35.0%
!! TTTCTAGTTTCTACACAGTC+AGG + chr3_1:85805196-85805215 Msa0383030:intron 35.0%
GATTCTACAAAGTGCAAGTT+AGG - chr3_1:85805289-85805308 None:intergenic 35.0%
GCCAAATCAATTTGAACTTC+TGG - chr3_1:85805328-85805347 None:intergenic 35.0%
CAACAAAGGTAAGCTATCTT+TGG + chr3_1:85805352-85805371 Msa0383030:intron 35.0%
! ATTGTAGCATTGGAAAGTAG+TGG + chr3_1:85805432-85805451 Msa0383030:intron 35.0%
!! GGTTGTGAGACTTTGATTTT+AGG - chr3_1:85805477-85805496 None:intergenic 35.0%
TCACAACCATCATTGATATG+AGG + chr3_1:85805489-85805508 Msa0383030:intron 35.0%
CACAACCATCATTGATATGA+GGG + chr3_1:85805490-85805509 Msa0383030:intron 35.0%
CATAACCCTCATATCAATGA+TGG - chr3_1:85805498-85805517 None:intergenic 35.0%
ACACAGCATTTAAACTCATG+AGG - chr3_1:85805596-85805615 None:intergenic 35.0%
! ACACGTGGATTAATGTTCAA+CGG - chr3_1:85805720-85805739 None:intergenic 35.0%
TTGATACGTCCATTAAGTGA+GGG - chr3_1:85806159-85806178 None:intergenic 35.0%
ATTGATACGTCCATTAAGTG+AGG - chr3_1:85806160-85806179 None:intergenic 35.0%
ACAAACAAAATCAATGCCTG+CGG - chr3_1:85806189-85806208 None:intergenic 35.0%
!!! ACTTTTTCAAGTGAAATGGG+TGG - chr3_1:85806228-85806247 None:intergenic 35.0%
!!! CAAGTTCAGATTTTTGAGAC+AGG + chr3_1:85806326-85806345 Msa0383030:three_prime_UTR 35.0%
CACATAACTAAGTCTGTGAT+TGG - chr3_1:85806355-85806374 None:intergenic 35.0%
TTGTAAACAACTTGCAGTTG+CGG + chr3_1:85806460-85806479 Msa0383030:three_prime_UTR 35.0%
TAACTCAATAAAAGGTTGGC+TGG - chr3_1:85806613-85806632 None:intergenic 35.0%
TCTTCTTTCGAACCACACTA+TGG + chr3_1:85803517-85803536 Msa0383030:exon 40.0%
! TCATCGTGTTTTTCTTCTGC+AGG + chr3_1:85803606-85803625 Msa0383030:intron 40.0%
!! GTAATCAACGGTTTGCTGAT+TGG - chr3_1:85803638-85803657 None:intergenic 40.0%
ATCAGTGATTCGTTAGGATC+TGG + chr3_1:85803742-85803761 Msa0383030:intron 40.0%
!! TTTGTGAAGAGGCATCTTAC+TGG + chr3_1:85803831-85803850 Msa0383030:CDS 40.0%
GTAAACAGCTACAATTGGTG+TGG + chr3_1:85803982-85804001 Msa0383030:intron 40.0%
CATAAACCGAACAGGGAATT+TGG - chr3_1:85804177-85804196 None:intergenic 40.0%
TGCAACACACCACGTAATAT+TGG - chr3_1:85804443-85804462 None:intergenic 40.0%
!! TAATAAGGGTGGCTGTTTCT+TGG + chr3_1:85804528-85804547 Msa0383030:intron 40.0%
TTATAATAGCACATTGCCCG+TGG - chr3_1:85804810-85804829 None:intergenic 40.0%
!! ACAAGGTTGATGTGAAGAAC+AGG + chr3_1:85805039-85805058 Msa0383030:CDS 40.0%
TTCCTAGCAAGGTAAAGGAA+TGG - chr3_1:85805155-85805174 None:intergenic 40.0%
CTTTGTAGAATCTCCTGCAT+TGG + chr3_1:85805297-85805316 Msa0383030:CDS 40.0%
!! TTGATTTGGCTGCACAACAA+AGG + chr3_1:85805338-85805357 Msa0383030:CDS 40.0%
! ATTTCTGGGGGCATATCATT+AGG + chr3_1:85805669-85805688 Msa0383030:intron 40.0%
AGGGATGATGAAAAAACACG+TGG - chr3_1:85805735-85805754 None:intergenic 40.0%
GTACTGCATATTCCTAAACG+AGG - chr3_1:85805776-85805795 None:intergenic 40.0%
ATCATCATCGTCATCAGGAA+GGG - chr3_1:85805967-85805986 None:intergenic 40.0%
AATGCATCATCATCGTCATC+AGG - chr3_1:85805972-85805991 None:intergenic 40.0%
! CGATGATGATGCATTTGAGT+AGG + chr3_1:85805977-85805996 Msa0383030:CDS 40.0%
! GTGATTGTGTTTTACCGTTC+AGG + chr3_1:85806372-85806391 Msa0383030:three_prime_UTR 40.0%
! AATAAAAGGTTGGCTGGAGA+AGG - chr3_1:85806607-85806626 None:intergenic 40.0%
! TTGAAAGCGTGAAACTGACG+CGG - chr3_1:85803423-85803442 None:intergenic 45.0%
! ACTGAGAGCATACCATAGTG+TGG - chr3_1:85803532-85803551 None:intergenic 45.0%
CTTGAAGCTCGGGTAATCAA+CGG - chr3_1:85803650-85803669 None:intergenic 45.0%
ACGATGACAAGCTTGAAGCT+CGG - chr3_1:85803661-85803680 None:intergenic 45.0%
AGCTTCAAGCTTGTCATCGT+CGG + chr3_1:85803661-85803680 Msa0383030:CDS 45.0%
CAAGATTCGCTTCTACTGCT+GGG + chr3_1:85804039-85804058 Msa0383030:CDS 45.0%
ACTGCTGGACAAGAGAAGTT+TGG + chr3_1:85804064-85804083 Msa0383030:CDS 45.0%
! AAGTTTGGTGGTCTCAGAGA+TGG + chr3_1:85804079-85804098 Msa0383030:CDS 45.0%
ACAAGCACAAACAATGCCGA+AGG - chr3_1:85804738-85804757 None:intergenic 45.0%
TGTGAAGAACAGGCAAGTGA+AGG + chr3_1:85805049-85805068 Msa0383030:CDS 45.0%
! CAAAGCAGGTTACTTTCCAC+AGG + chr3_1:85805072-85805091 Msa0383030:CDS 45.0%
AGCCATTCCTTTACCTTGCT+AGG + chr3_1:85805150-85805169 Msa0383030:CDS 45.0%
CAAGCTTCCTAGCAAGGTAA+AGG - chr3_1:85805160-85805179 None:intergenic 45.0%
AACGAGGCGGATAAAAGGAA+GGG - chr3_1:85805760-85805779 None:intergenic 45.0%
AAACGAGGCGGATAAAAGGA+AGG - chr3_1:85805761-85805780 None:intergenic 45.0%
! TCCTTTTATCCGCCTCGTTT+AGG + chr3_1:85805761-85805780 Msa0383030:intron 45.0%
TCCTAAACGAGGCGGATAAA+AGG - chr3_1:85805765-85805784 None:intergenic 45.0%
CTGCATATTCCTAAACGAGG+CGG - chr3_1:85805773-85805792 None:intergenic 45.0%
TCATCATCGTCATCAGGAAG+GGG - chr3_1:85805966-85805985 None:intergenic 45.0%
CATCATCATCGTCATCAGGA+AGG - chr3_1:85805968-85805987 None:intergenic 45.0%
TGCTATCCTTTGTCTCGAGT+TGG + chr3_1:85806011-85806030 Msa0383030:three_prime_UTR 45.0%
CGATGACAAGCTTGAAGCTC+GGG - chr3_1:85803660-85803679 None:intergenic 50.0%
! TGGTGTGGAAGTGCATCCAT+TGG + chr3_1:85803997-85804016 Msa0383030:CDS 50.0%
GCAAGATTCGCTTCTACTGC+TGG + chr3_1:85804038-85804057 Msa0383030:CDS 50.0%
TTCTACTGCTGGGATACTGC+TGG + chr3_1:85804049-85804068 Msa0383030:CDS 50.0%
GCTGGACAAGAGAAGTTTGG+TGG + chr3_1:85804067-85804086 Msa0383030:CDS 50.0%
TACCTTGCTAGGAAGCTTGC+AGG + chr3_1:85805161-85805180 Msa0383030:CDS 50.0%
ACCTTGCTAGGAAGCTTGCA+GGG + chr3_1:85805162-85805181 Msa0383030:CDS 50.0%
GCGGATAAAAGGAAGGGACA+GGG - chr3_1:85805754-85805773 None:intergenic 50.0%
! GTCATCAGGAAGGGGTTGAT+TGG - chr3_1:85805958-85805977 None:intergenic 50.0%
ACAAAGGATAGCAGTCGCAC+GGG - chr3_1:85806004-85806023 None:intergenic 50.0%
GACAAAGGATAGCAGTCGCA+CGG - chr3_1:85806005-85806024 None:intergenic 50.0%
ACAGCACCAACTCGAGACAA+AGG - chr3_1:85806020-85806039 None:intergenic 50.0%
ACGTATCAATACTCTGCCGC+AGG + chr3_1:85806170-85806189 Msa0383030:three_prime_UTR 50.0%
GACGCGGCTCTTCTGTCTTT+CGG - chr3_1:85803407-85803426 None:intergenic 55.0%
AAGCTTGTCATCGTCGGCGA+TGG + chr3_1:85803667-85803686 Msa0383030:CDS 55.0%
! CAAAGATCACGGTGCCAGGT+AGG - chr3_1:85804871-85804890 None:intergenic 55.0%
CCTGGCACCGTGATCTTTGT+CGG + chr3_1:85804872-85804891 Msa0383030:CDS 55.0%
CAGGCAAGTGAAGGCAAAGC+AGG + chr3_1:85805058-85805077 Msa0383030:CDS 55.0%
ACCCTGCAAGCTTCCTAGCA+AGG - chr3_1:85805166-85805185 None:intergenic 55.0%
TGCTAGGAAGCTTGCAGGGT+AGG + chr3_1:85805166-85805185 Msa0383030:intron 55.0%
ACTTCTGGTGGAGCCAATGC+AGG - chr3_1:85805313-85805332 None:intergenic 55.0%
GGCGGATAAAAGGAAGGGAC+AGG - chr3_1:85805755-85805774 None:intergenic 55.0%
! GAAGGGGTTGATTGGCAGCT+TGG - chr3_1:85805950-85805969 None:intergenic 55.0%
CTTGTCATCGTCGGCGATGG+CGG + chr3_1:85803670-85803689 Msa0383030:CDS 60.0%
CCGACAAAGATCACGGTGCC+AGG - chr3_1:85804875-85804894 None:intergenic 60.0%
! ATCGTCGGCGATGGCGGCAC+TGG + chr3_1:85803676-85803695 Msa0383030:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 85803374 85806656 85803374 ID=Msa0383030;Name=Msa0383030
chr3_1 mRNA 85803374 85806656 85803374 ID=Msa0383030-mRNA-1;Parent=Msa0383030;Name=Msa0383030-mRNA-1;_AED=0.34;_eAED=0.34;_QI=162|1|1|1|1|1|8|658|221
chr3_1 exon 85803374 85803538 85803374 ID=Msa0383030-mRNA-1:exon:22622;Parent=Msa0383030-mRNA-1
chr3_1 exon 85803628 85803697 85803628 ID=Msa0383030-mRNA-1:exon:22623;Parent=Msa0383030-mRNA-1
chr3_1 exon 85803820 85803876 85803820 ID=Msa0383030-mRNA-1:exon:22624;Parent=Msa0383030-mRNA-1
chr3_1 exon 85803990 85804107 85803990 ID=Msa0383030-mRNA-1:exon:22625;Parent=Msa0383030-mRNA-1
chr3_1 exon 85804804 85804893 85804804 ID=Msa0383030-mRNA-1:exon:22626;Parent=Msa0383030-mRNA-1
chr3_1 exon 85805004 85805183 85805004 ID=Msa0383030-mRNA-1:exon:22627;Parent=Msa0383030-mRNA-1
chr3_1 exon 85805282 85805359 85805282 ID=Msa0383030-mRNA-1:exon:22628;Parent=Msa0383030-mRNA-1
chr3_1 exon 85805929 85806656 85805929 ID=Msa0383030-mRNA-1:exon:22629;Parent=Msa0383030-mRNA-1
chr3_1 five_prime_UTR 85803374 85803535 85803374 ID=Msa0383030-mRNA-1:five_prime_utr;Parent=Msa0383030-mRNA-1
chr3_1 CDS 85803536 85803538 85803536 ID=Msa0383030-mRNA-1:cds;Parent=Msa0383030-mRNA-1
chr3_1 CDS 85803628 85803697 85803628 ID=Msa0383030-mRNA-1:cds;Parent=Msa0383030-mRNA-1
chr3_1 CDS 85803820 85803876 85803820 ID=Msa0383030-mRNA-1:cds;Parent=Msa0383030-mRNA-1
chr3_1 CDS 85803990 85804107 85803990 ID=Msa0383030-mRNA-1:cds;Parent=Msa0383030-mRNA-1
chr3_1 CDS 85804804 85804893 85804804 ID=Msa0383030-mRNA-1:cds;Parent=Msa0383030-mRNA-1
chr3_1 CDS 85805004 85805183 85805004 ID=Msa0383030-mRNA-1:cds;Parent=Msa0383030-mRNA-1
chr3_1 CDS 85805282 85805359 85805282 ID=Msa0383030-mRNA-1:cds;Parent=Msa0383030-mRNA-1
chr3_1 CDS 85805929 85805998 85805929 ID=Msa0383030-mRNA-1:cds;Parent=Msa0383030-mRNA-1
chr3_1 three_prime_UTR 85805999 85806656 85805999 ID=Msa0383030-mRNA-1:three_prime_utr;Parent=Msa0383030-mRNA-1
Gene Sequence

>Msa0383030

ATGGCTTTGCCCAATCAGCAAACCGTTGATTACCCGAGCTTCAAGCTTGTCATCGTCGGCGATGGCGGCACTGGAAAGACAACATTTGTGAAGAGGCATCTTACTGGAGAATTTGAGAAGAAATATGAACCTACAATTGGTGTGGAAGTGCATCCATTGGATTTCTTCACAAACTGCGGCAAGATTCGCTTCTACTGCTGGGATACTGCTGGACAAGAGAAGTTTGGTGGTCTCAGAGATGGATACTATATCCACGGGCAATGTGCTATTATAATGTTTGATGTTACTGCCCGTCTAACATACAAAAATGTTCCTACCTGGCACCGTGATCTTTGTCGGGTCTGTGAAAACATCCCAATTGTTCTTTGCGGTAACAAGGTTGATGTGAAGAACAGGCAAGTGAAGGCAAAGCAGGTTACTTTCCACAGGAAGAAAAATTTGCAGTACTATGAGATTTCAGCTAAGAGCAACTATAACTTTGAGAAGCCATTCCTTTACCTTGCTAGGAAGCTTGCAGGTGACCCTAACTTGCACTTTGTAGAATCTCCTGCATTGGCTCCACCAGAAGTTCAAATTGATTTGGCTGCACAACAAAGACATGAGAATGAGCTTCTCCAAGCTGCCAATCAACCCCTTCCTGATGACGATGATGATGCATTTGAGTAG
Protein sequence

>Msa0383030

MALPNQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQIDLAAQQRHENELLQAANQPLPDDDDDAFE*