AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0433010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0433010 MtrunA17_Chr3g0138101 99.548 221 1 0 1 221 1 221 4.20e-168 461
Msa0433010 MtrunA17_Chr3g0138121 98.643 221 3 0 1 221 1 221 6.05e-167 458
Msa0433010 MtrunA17_Chr3g0138111 98.643 221 3 0 1 221 1 221 1.59e-166 457
Msa0433010 MtrunA17_Chr4g0063211 72.993 137 25 1 85 221 7 131 1.18e-66 201
Msa0433010 MtrunA17_Chr4g0055081 30.052 193 128 4 14 203 10 198 3.80e-27 103
Msa0433010 MtrunA17_Chr4g0046261 30.526 190 126 4 14 197 14 203 2.98e-26 100
Msa0433010 MtrunA17_Chr5g0400131 31.977 172 106 3 14 178 10 177 3.73e-26 100
Msa0433010 MtrunA17_Chr3g0109721 33.526 173 109 4 15 184 12 181 7.23e-26 99.8
Msa0433010 MtrunA17_Chr8g0390561 34.161 161 103 3 15 172 11 171 8.08e-26 99.4
Msa0433010 MtrunA17_Chr2g0283131 30.526 190 126 4 14 197 14 203 1.33e-25 99.4
Msa0433010 MtrunA17_Chr8g0383261 30.723 166 105 3 14 169 13 178 1.41e-25 99.0
Msa0433010 MtrunA17_Chr1g0201121 27.749 191 125 4 14 191 9 199 1.63e-25 99.0
Msa0433010 MtrunA17_Chr8g0349711 32.716 162 106 3 13 171 6 167 3.20e-25 98.2
Msa0433010 MtrunA17_Chr1g0159481 37.647 170 98 6 14 177 18 185 4.85e-25 97.8
Msa0433010 MtrunA17_Chr4g0032201 31.844 179 114 3 14 189 13 186 5.68e-25 97.8
Msa0433010 MtrunA17_Chr8g0349681 33.951 162 104 3 13 171 175 336 6.09e-25 102
Msa0433010 MtrunA17_Chr8g0349681 32.515 163 107 3 13 172 13 175 2.41e-22 94.7
Msa0433010 MtrunA17_Chr8g0349681 32.298 161 106 2 14 171 385 545 5.72e-22 93.6
Msa0433010 MtrunA17_Chr8g0381841 31.915 188 119 5 7 191 12 193 8.84e-25 97.4
Msa0433010 MtrunA17_Chr8g0384311 34.637 179 110 5 4 177 5 181 1.04e-24 96.7
Msa0433010 MtrunA17_Chr3g0123971 32.143 168 111 3 14 178 10 177 1.05e-24 96.7
Msa0433010 MtrunA17_Chr4g0040781 30.000 190 127 4 14 197 14 203 1.24e-24 96.7
Msa0433010 MtrunA17_Chr3g0112681 34.731 167 105 4 1 164 1 166 1.34e-24 96.7
Msa0433010 MtrunA17_Chr1g0182321 29.381 194 128 5 2 190 3 192 1.43e-24 96.7
Msa0433010 MtrunA17_Chr5g0433561 32.948 173 110 4 15 184 12 181 1.53e-24 96.3
Msa0433010 MtrunA17_Chr6g0482721 34.483 174 110 4 1 171 1 173 1.74e-24 96.3
Msa0433010 MtrunA17_Chr4g0055651 30.435 184 119 4 14 192 9 188 2.20e-24 95.9
Msa0433010 MtrunA17_Chr1g0182301 28.866 194 129 5 2 190 3 192 4.95e-24 95.1
Msa0433010 MtrunA17_Chr2g0319951 33.117 154 100 2 14 164 14 167 5.62e-24 95.1
Msa0433010 MtrunA17_Chr2g0325091 32.903 155 101 2 14 165 12 166 5.67e-24 95.1
Msa0433010 MtrunA17_Chr1g0212521 30.247 162 109 3 14 171 16 177 6.37e-24 94.7
Msa0433010 MtrunA17_Chr4g0030911 30.247 162 109 3 14 171 16 177 7.69e-24 94.7
Msa0433010 MtrunA17_Chr8g0382221 32.143 168 111 3 14 178 10 177 8.58e-24 94.4
Msa0433010 MtrunA17_Chr2g0276941 34.416 154 98 2 14 164 16 169 9.33e-24 94.7
Msa0433010 MtrunA17_Chr6g0466301 27.513 189 124 4 14 189 9 197 9.42e-24 94.4
Msa0433010 MtrunA17_Chr6g0465491 27.513 189 124 4 14 189 9 197 9.42e-24 94.4
Msa0433010 MtrunA17_Chr3g0123821 29.126 206 129 5 7 198 11 213 1.20e-23 94.4
Msa0433010 MtrunA17_Chr7g0263071 30.247 162 109 3 14 171 16 177 1.32e-23 94.0
Msa0433010 MtrunA17_Chr2g0315551 32.468 154 101 2 14 164 14 167 1.58e-23 94.0
Msa0433010 MtrunA17_Chr6g0458081 32.298 161 106 2 14 171 7 167 2.66e-23 93.2
Msa0433010 MtrunA17_Chr8g0351861 31.765 170 112 4 5 171 9 177 3.51e-23 93.2
Msa0433010 MtrunA17_Chr8g0335471 32.447 188 111 6 14 192 9 189 6.13e-23 92.0
Msa0433010 MtrunA17_Chr7g0275991 33.333 168 108 3 7 171 15 181 7.87e-23 92.4
Msa0433010 MtrunA17_Chr7g0265001 35.088 171 104 5 13 177 16 185 8.05e-23 92.0
Msa0433010 MtrunA17_Chr7g0250531 30.108 186 117 4 14 192 9 188 1.04e-22 91.3
Msa0433010 MtrunA17_Chr4g0034871 31.214 173 95 6 14 169 9 174 1.10e-22 91.3
Msa0433010 MtrunA17_Chr3g0130271 34.973 183 111 6 1 177 1 181 1.59e-22 90.9
Msa0433010 MtrunA17_Chr7g0214241 31.056 161 108 3 14 171 16 176 1.86e-22 91.3
Msa0433010 MtrunA17_Chr2g0331691 31.677 161 100 3 14 164 11 171 2.83e-22 90.5
Msa0433010 MtrunA17_Chr8g0392931 33.121 157 102 2 14 167 15 171 3.15e-22 90.5
Msa0433010 MtrunA17_Chr2g0327911 28.834 163 111 2 14 171 16 178 6.30e-22 89.7
Msa0433010 MtrunA17_Chr8g0346421 33.136 169 109 4 6 171 20 187 8.69e-22 89.7
Msa0433010 MtrunA17_Chr4g0004661 31.792 173 94 6 14 169 65 230 9.01e-22 90.1
Msa0433010 MtrunA17_Chr1g0180291 32.749 171 107 6 13 177 14 182 1.11e-21 89.0
Msa0433010 MtrunA17_Chr1g0174281 31.098 164 109 3 7 167 10 172 2.34e-21 88.2
Msa0433010 MtrunA17_Chr7g0231131 32.716 162 105 4 9 167 12 172 2.72e-21 88.2
Msa0433010 MtrunA17_Chr2g0290891 31.646 158 105 2 14 168 7 164 3.13e-21 87.8
Msa0433010 MtrunA17_Chr1g0189361 30.114 176 117 5 1 171 1 175 1.19e-20 86.3
Msa0433010 MtrunA17_Chr1g0199331 29.193 161 110 3 15 171 33 193 4.62e-20 84.3
Msa0433010 MtrunA17_Chr7g0234201 28.571 161 112 3 14 171 14 174 4.73e-20 84.7
Msa0433010 MtrunA17_Chr2g0276931 30.061 163 107 4 14 171 10 170 1.07e-19 83.6
Msa0433010 MtrunA17_Chr1g0197331 29.442 197 123 7 10 199 13 200 7.80e-19 81.3
Msa0433010 MtrunA17_Chr8g0371501 28.485 165 103 4 14 164 7 170 3.92e-14 68.6
Msa0433010 MtrunA17_Chr6g0486041 33.051 118 77 2 14 130 7 123 5.34e-14 68.2
Msa0433010 MtrunA17_Chr5g0405791 28.485 165 103 4 14 164 7 170 9.04e-14 67.4
Msa0433010 MtrunA17_Chr4g0046211 30.252 119 81 2 14 131 7 124 9.33e-14 66.2
Msa0433010 MtrunA17_Chr6g0466161 27.815 151 99 3 29 169 6 156 1.60e-13 67.0
Msa0433010 MtrunA17_Chr6g0465471 27.815 151 99 3 29 169 6 156 1.98e-13 66.6
Msa0433010 MtrunA17_Chr5g0443631 26.012 173 121 1 10 175 101 273 1.53e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0433010 AT5G55190.1 96.833 221 7 0 1 221 1 221 7.36e-164 451
Msa0433010 AT5G20010.1 95.475 221 10 0 1 221 1 221 4.71e-161 444
Msa0433010 AT5G20020.1 95.023 221 11 0 1 221 1 221 1.41e-152 422
Msa0433010 AT5G55080.1 70.283 212 58 1 1 212 1 207 1.37e-108 311
Msa0433010 AT4G39890.1 36.158 177 103 4 14 186 10 180 4.84e-28 105
Msa0433010 AT1G07410.1 31.551 187 120 3 14 197 13 194 4.45e-26 100
Msa0433010 AT4G19640.1 33.514 185 116 5 15 195 12 193 5.22e-26 100
Msa0433010 AT2G44610.1 32.143 168 111 3 14 178 10 177 9.85e-26 99.8
Msa0433010 AT4G18430.1 32.298 161 106 3 14 171 14 174 2.46e-25 99.0
Msa0433010 AT5G45130.1 33.140 172 109 4 15 183 12 180 2.54e-25 98.6
Msa0433010 AT5G39620.1 32.335 167 103 3 14 170 6 172 3.30e-25 98.2
Msa0433010 AT5G59150.1 34.731 167 105 3 1 164 1 166 7.86e-25 97.8
Msa0433010 AT5G45750.1 35.256 156 94 3 14 164 14 167 9.91e-25 97.4
Msa0433010 AT4G18800.1 32.597 181 110 4 14 189 14 187 1.54e-24 96.7
Msa0433010 AT1G09630.1 34.132 167 106 3 1 164 1 166 1.66e-24 96.7
Msa0433010 AT2G21880.1 30.909 165 104 3 14 168 10 174 1.94e-24 96.7
Msa0433010 AT5G65270.1 31.217 189 121 5 6 191 11 193 2.87e-24 96.3
Msa0433010 AT5G60860.1 30.000 190 127 4 14 197 14 203 3.17e-24 96.3
Msa0433010 AT2G22290.1 32.515 163 107 3 14 173 10 172 3.48e-24 95.9
Msa0433010 AT1G18200.1 32.919 161 105 3 14 171 14 174 3.50e-24 96.3
Msa0433010 AT4G09720.4 29.240 171 106 3 14 169 9 179 3.89e-24 95.9
Msa0433010 AT4G39990.1 33.333 168 104 4 9 171 14 178 4.70e-24 95.9
Msa0433010 AT3G15060.1 33.766 154 99 3 14 164 14 167 4.73e-24 95.5
Msa0433010 AT4G09720.1 28.916 166 108 3 14 169 9 174 5.19e-24 95.1
Msa0433010 AT3G18820.1 30.882 204 113 7 14 200 9 201 6.21e-24 95.1
Msa0433010 AT1G22740.1 29.630 189 120 6 14 189 9 197 6.38e-24 95.1
Msa0433010 AT3G53610.2 28.729 181 121 4 14 190 16 192 6.76e-24 95.1
Msa0433010 AT3G53610.3 28.729 181 121 4 14 190 16 192 6.76e-24 95.1
Msa0433010 AT3G53610.1 28.729 181 121 4 14 190 16 192 6.76e-24 95.1
Msa0433010 AT5G59840.1 29.319 191 123 5 14 196 16 202 6.83e-24 95.1
Msa0433010 AT5G10260.1 32.738 168 110 3 14 178 10 177 1.33e-23 94.4
Msa0433010 AT3G46830.1 34.783 161 101 3 7 164 7 166 1.57e-23 94.4
Msa0433010 AT3G46830.2 34.783 161 101 3 7 164 7 166 1.57e-23 94.4
Msa0433010 AT5G45130.2 32.353 170 109 4 17 183 22 188 1.68e-23 94.0
Msa0433010 AT3G46060.2 30.247 162 109 3 14 171 16 177 2.42e-23 93.6
Msa0433010 AT3G46060.3 30.247 162 109 3 14 171 16 177 2.42e-23 93.6
Msa0433010 AT3G46060.1 30.247 162 109 3 14 171 16 177 2.42e-23 93.6
Msa0433010 AT4G09720.3 28.249 177 106 3 14 169 9 185 2.42e-23 94.0
Msa0433010 AT4G17530.1 30.435 184 119 4 14 192 9 188 5.32e-23 92.4
Msa0433010 AT5G03530.2 32.353 170 109 4 13 177 13 181 6.62e-23 92.4
Msa0433010 AT5G03530.1 32.353 170 109 4 13 177 13 181 6.62e-23 92.4
Msa0433010 AT5G47200.1 30.435 184 119 4 14 192 9 188 7.45e-23 92.0
Msa0433010 AT1G73640.1 32.544 169 110 4 6 171 7 174 9.24e-23 92.8
Msa0433010 AT3G12160.1 32.927 164 106 4 7 167 10 172 1.01e-22 92.4
Msa0433010 AT5G47960.1 33.951 162 103 4 7 165 10 170 1.02e-22 92.4
Msa0433010 AT5G64990.1 28.079 203 124 6 14 203 8 201 1.63e-22 91.3
Msa0433010 AT2G33870.1 30.270 185 123 4 14 192 14 198 1.94e-22 91.3
Msa0433010 AT3G16100.1 29.798 198 123 5 14 200 9 201 2.14e-22 90.9
Msa0433010 AT1G28550.1 29.730 185 124 4 14 192 14 198 2.34e-22 91.3
Msa0433010 AT1G06400.1 33.766 154 99 3 14 164 14 167 2.47e-22 91.3
Msa0433010 AT1G02130.1 30.811 185 118 5 14 192 9 189 3.26e-22 90.5
Msa0433010 AT1G49300.2 31.977 172 93 6 14 168 9 173 3.44e-22 90.5
Msa0433010 AT1G49300.1 31.977 172 93 6 14 168 9 173 3.44e-22 90.5
Msa0433010 AT1G52280.1 29.293 198 124 6 14 200 9 201 4.25e-22 90.1
Msa0433010 AT1G43890.3 33.136 169 107 4 14 177 14 181 4.30e-22 90.5
Msa0433010 AT1G43890.1 33.136 169 107 4 14 177 14 181 4.30e-22 90.5
Msa0433010 AT1G43890.2 33.136 169 107 4 14 177 14 181 4.30e-22 90.5
Msa0433010 AT1G16920.1 31.818 154 102 2 14 164 14 167 4.88e-22 90.5
Msa0433010 AT2G43130.1 27.461 193 136 4 14 203 13 204 7.20e-22 89.7
Msa0433010 AT4G35860.1 31.056 161 108 2 14 171 7 167 7.75e-22 89.7
Msa0433010 AT1G01200.1 31.953 169 110 4 7 171 23 190 1.03e-21 90.1
Msa0433010 AT4G17160.1 30.380 158 107 2 14 168 7 164 3.19e-21 87.8
Msa0433010 AT4G17170.1 31.056 161 108 2 14 171 7 167 6.37e-21 87.4
Msa0433010 AT3G07410.1 29.310 174 120 3 14 184 13 186 1.19e-20 86.7
Msa0433010 AT1G05810.1 30.519 154 104 3 14 164 13 166 1.35e-20 86.7
Msa0433010 AT5G47520.1 30.435 161 109 3 14 171 15 175 1.46e-20 86.7
Msa0433010 AT1G05810.2 30.519 154 104 3 14 164 59 212 3.90e-20 86.3
Msa0433010 AT4G19640.2 35.938 128 80 2 15 140 12 139 5.46e-20 83.2
Msa0433010 AT4G19640.3 34.043 141 90 3 15 152 12 152 6.85e-20 84.0
Msa0433010 AT5G64990.2 27.143 210 124 7 14 203 8 208 8.94e-20 84.3
Msa0433010 AT3G54840.1 29.814 161 109 4 15 171 35 195 9.68e-20 84.0
Msa0433010 AT5G03520.1 28.272 191 128 5 4 189 5 191 1.70e-19 83.6
Msa0433010 AT2G31680.1 29.193 161 111 3 14 171 13 173 1.73e-19 83.6
Msa0433010 AT3G11730.1 28.108 185 126 3 14 191 9 193 2.86e-19 82.8
Msa0433010 AT3G54840.3 29.375 160 109 4 16 171 2 161 3.64e-19 81.6
Msa0433010 AT3G09900.1 28.272 191 128 5 4 189 5 191 3.77e-19 82.8
Msa0433010 AT3G54840.2 30.519 154 103 4 15 164 35 188 1.64e-18 80.5
Msa0433010 AT3G09910.3 31.176 170 111 4 13 177 13 181 1.93e-17 77.8
Msa0433010 AT3G09910.1 31.176 170 111 4 13 177 13 181 1.93e-17 77.8
Msa0433010 AT3G09910.2 31.176 170 111 4 13 177 13 181 1.93e-17 77.8
Msa0433010 AT2G21880.4 29.530 149 95 3 30 168 1 149 2.68e-17 77.0
Msa0433010 AT2G21880.3 29.530 149 95 3 30 168 1 149 2.68e-17 77.0
Msa0433010 AT3G09910.5 31.098 164 107 4 13 171 13 175 1.00e-16 75.9
Msa0433010 AT3G09910.4 31.098 164 107 4 13 171 13 175 1.00e-16 75.9
Msa0433010 AT4G09720.6 28.777 139 89 3 41 169 2 140 2.00e-16 74.3
Msa0433010 AT4G09720.5 28.777 139 89 3 41 169 2 140 2.00e-16 74.3
Msa0433010 AT4G09720.2 28.777 139 89 3 41 169 2 140 2.00e-16 74.3
Msa0433010 AT2G21880.2 29.091 165 99 5 14 168 10 166 3.70e-16 74.3
Msa0433010 AT4G35860.2 31.858 113 74 2 62 171 9 121 6.07e-15 70.1
Msa0433010 AT5G03520.2 29.323 133 86 4 61 189 53 181 6.72e-15 70.9
Msa0433010 AT5G59150.2 33.962 106 67 2 62 164 10 115 5.76e-14 67.8
Msa0433010 AT4G35950.1 29.697 165 101 4 14 164 7 170 6.57e-14 68.2
Msa0433010 AT2G17800.2 31.933 119 79 2 14 131 7 124 9.19e-14 67.8
Msa0433010 AT2G17800.1 31.933 119 79 2 14 131 7 124 9.19e-14 67.8
Msa0433010 AT3G51300.1 32.203 118 78 2 15 131 8 124 1.09e-13 67.4
Msa0433010 AT4G28950.1 28.834 163 103 4 14 164 7 168 1.38e-13 67.4
Msa0433010 AT4G35020.4 30.702 114 77 2 15 127 8 120 7.77e-11 58.5
Msa0433010 AT4G35020.5 30.702 114 77 2 15 127 8 120 7.77e-11 58.5

Find 74 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGTAAACCCTCACTTAA+TGG 0.159303 3_2:+85335788 Msa0433010:three_prime_UTR
GAGAAGTGAATAAACGAATT+AGG 0.189362 3_2:-85333108 None:intergenic
GCCAAATCAATTTGAACTTC+TGG 0.258290 3_2:-85334966 None:intergenic
TTGCAATCTGTCTCTTCAAT+TGG 0.267988 3_2:-85333013 None:intergenic
GATTCTACAAAGTGCAAGTT+AGG 0.300639 3_2:-85334927 None:intergenic
AGAAGTGAATAAACGAATTA+GGG 0.313376 3_2:-85333107 None:intergenic
ACCAGAAGTTCAAATTGATT+TGG 0.314372 3_2:+85334965 Msa0433010:CDS
TTTGTGAAGAGGCATCTTAC+TGG 0.332899 3_2:+85333473 Msa0433010:CDS
TAATCAACGGTTTGCTGATT+GGG 0.341509 3_2:-85333276 None:intergenic
TACCTTGCTAGGAAGCTTGC+AGG 0.343483 3_2:+85334802 Msa0433010:CDS
TGCAATCTGTCTCTTCAATT+GGG 0.359194 3_2:-85333012 None:intergenic
GACGCGGCTCTTCTGTCTTT+CGG 0.366086 3_2:-85333042 None:intergenic
GCAAGATTCGCTTCTACTGC+TGG 0.380027 3_2:+85333680 Msa0433010:CDS
TCTTCTTTCGAACCACACTA+TGG 0.402104 3_2:+85333159 Msa0433010:exon
TTGTTACCGCAAAGAACAAT+TGG 0.403385 3_2:-85334661 None:intergenic
AACATCCCAATTGTTCTTTG+CGG 0.404004 3_2:+85334655 Msa0433010:CDS
GTAATCAACGGTTTGCTGAT+TGG 0.420523 3_2:-85333277 None:intergenic
GAAGGGGTTGATTGGCAGCT+TGG 0.435439 3_2:-85335588 None:intergenic
CCTGGCACCGTGATCTTTGT+CGG 0.450109 3_2:+85334513 Msa0433010:CDS
GTCATCAGGAAGGGGTTGAT+TGG 0.454313 3_2:-85335596 None:intergenic
CTTTGTAGAATCTCCTGCAT+TGG 0.466850 3_2:+85334938 Msa0433010:CDS
CAGGCAAGTGAAGGCAAAGC+AGG 0.468849 3_2:+85334699 Msa0433010:CDS
ACTGCTGGACAAGAGAAGTT+TGG 0.471338 3_2:+85333706 Msa0433010:CDS
ACAAGGTTGATGTGAAGAAC+AGG 0.475162 3_2:+85334680 Msa0433010:CDS
CGATGACAAGCTTGAAGCTC+GGG 0.476801 3_2:-85333299 None:intergenic
TGCTATCCTTTGTCTCGAGT+TGG 0.480872 3_2:+85335652 Msa0433010:three_prime_UTR
ATCGTCGGCGATGGCGGCAC+TGG 0.486311 3_2:+85333318 Msa0433010:CDS
CTTGTCATCGTCGGCGATGG+CGG 0.488335 3_2:+85333312 Msa0433010:CDS
AATGCATCATCATCGTCATC+AGG 0.495924 3_2:-85335610 None:intergenic
TGATTGTAAACAGCTACAAT+TGG 0.508837 3_2:+85333619 Msa0433010:intron
CAAGCTTCCTAGCAAGGTAA+AGG 0.519388 3_2:-85334798 None:intergenic
CTTTATATTGCAGTATCCAC+GGG 0.523043 3_2:+85334432 Msa0433010:intron
CCGACAAAGATCACGGTGCC+AGG 0.524098 3_2:-85334513 None:intergenic
AAGCTTGTCATCGTCGGCGA+TGG 0.529447 3_2:+85333309 Msa0433010:CDS
TCTTTATATTGCAGTATCCA+CGG 0.531789 3_2:+85334431 Msa0433010:intron
ACTTCTGGTGGAGCCAATGC+AGG 0.537856 3_2:-85334951 None:intergenic
AGCTTCAAGCTTGTCATCGT+CGG 0.540576 3_2:+85333303 Msa0433010:CDS
ACGTATCAATACTCTGCTGC+AGG 0.542060 3_2:+85335811 Msa0433010:three_prime_UTR
AAGTTTGGTGGTCTCAGAGA+TGG 0.543632 3_2:+85333721 Msa0433010:CDS
TTCTACTGCTGGGATACTGC+TGG 0.543775 3_2:+85333691 Msa0433010:CDS
AATTGTTCTTTGCGGTAACA+AGG 0.546634 3_2:+85334663 Msa0433010:CDS
CAAAGCAGGTTACTTTCCAC+AGG 0.547056 3_2:+85334713 Msa0433010:CDS
CATCATCATCGTCATCAGGA+AGG 0.549641 3_2:-85335606 None:intergenic
TGTTACCGCAAAGAACAATT+GGG 0.552031 3_2:-85334660 None:intergenic
ATCATCATCGTCATCAGGAA+GGG 0.552321 3_2:-85335605 None:intergenic
AGCCATTCCTTTACCTTGCT+AGG 0.553251 3_2:+85334791 Msa0433010:CDS
AAATCAATTTGAACTTCTGG+TGG 0.563330 3_2:-85334963 None:intergenic
CAAGATTCGCTTCTACTGCT+GGG 0.564996 3_2:+85333681 Msa0433010:CDS
TTGATTTGGCTGCACAACAA+AGG 0.579779 3_2:+85334979 Msa0433010:CDS
TTGATACGTCCATTAAGTGA+GGG 0.582936 3_2:-85335797 None:intergenic
GACAAAGGATAGCAGTCGCA+CGG 0.584056 3_2:-85335643 None:intergenic
CTTGAAGCTCGGGTAATCAA+CGG 0.586019 3_2:-85333289 None:intergenic
ACCCTGCAAGCTTCCTAGCA+AGG 0.586472 3_2:-85334804 None:intergenic
TTGGATTTCTTCACAAACTG+CGG 0.590990 3_2:+85333658 Msa0433010:CDS
ACGATGACAAGCTTGAAGCT+CGG 0.595291 3_2:-85333300 None:intergenic
GAAAGACAACATTTGTGAAG+AGG 0.596908 3_2:+85333462 Msa0433010:CDS
ATTCTACAAAGTGCAAGTTA+GGG 0.598867 3_2:-85334926 None:intergenic
ATTGATACGTCCATTAAGTG+AGG 0.602666 3_2:-85335798 None:intergenic
TGGTGTGGAAGTGCATCCAT+TGG 0.604734 3_2:+85333639 Msa0433010:CDS
TTCCTAGCAAGGTAAAGGAA+TGG 0.606313 3_2:-85334793 None:intergenic
CAAAGATCACGGTGCCAGGT+AGG 0.607872 3_2:-85334509 None:intergenic
ACAAAGGATAGCAGTCGCAC+GGG 0.609636 3_2:-85335642 None:intergenic
CAGTTTGTGAAGAAATCCAA+TGG 0.610326 3_2:-85333655 None:intergenic
ACCTTGCTAGGAAGCTTGCA+GGG 0.610380 3_2:+85334803 Msa0433010:CDS
TGTGAAGAACAGGCAAGTGA+AGG 0.615131 3_2:+85334690 Msa0433010:CDS
GCTGGACAAGAGAAGTTTGG+TGG 0.616663 3_2:+85333709 Msa0433010:CDS
TCATCATCGTCATCAGGAAG+GGG 0.618873 3_2:-85335604 None:intergenic
ACAGCACCAACTCGAGACAA+AGG 0.634897 3_2:-85335658 None:intergenic
TTATAATAGCACATTGCCCG+TGG 0.636385 3_2:-85334448 None:intergenic
CGATGATGATGCATTTGAGT+AGG 0.644342 3_2:+85335618 Msa0433010:CDS
GTAAACAGCTACAATTGGTG+TGG 0.644898 3_2:+85333624 Msa0433010:intron
TTACTAACCGACAAAGATCA+CGG 0.655820 3_2:-85334520 None:intergenic
ACTGAGAGCATACCATAGTG+TGG 0.696735 3_2:-85333171 None:intergenic
TTGAAAGCGTGAAACTGACG+CGG 0.697502 3_2:-85333058 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAAGATTCATTTTAATTTA+GGG + chr3_2:85333519-85333538 Msa0433010:intron 15.0%
!! AAAAAAAAAGTCTACATTTA+TGG - chr3_2:85334144-85334163 None:intergenic 15.0%
!!! TTTTTTTTTCGTGTTAATAA+GGG + chr3_2:85334155-85334174 Msa0433010:intron 15.0%
!!! ATTTTGAATTTCTCATTTAA+TGG + chr3_2:85335536-85335555 Msa0433010:intron 15.0%
!!! TTAGGGTTTTTATATTACAA+GGG - chr3_2:85333093-85333112 None:intergenic 20.0%
!!! ATTAGGGTTTTTATATTACA+AGG - chr3_2:85333094-85333113 None:intergenic 20.0%
!!! AATTGTTATTGTTAATCGAA+CGG - chr3_2:85333229-85333248 None:intergenic 20.0%
!! ATTCTATGTTACATATTTCA+CGG - chr3_2:85333366-85333385 None:intergenic 20.0%
!!! AATAATGATCATTTTCTTAC+AGG + chr3_2:85333440-85333459 Msa0433010:intron 20.0%
!!! CGTAAGATTCATTTTAATTT+AGG + chr3_2:85333518-85333537 Msa0433010:intron 20.0%
!! TTAACAAGCATGATTATATA+CGG + chr3_2:85333542-85333561 Msa0433010:intron 20.0%
!!! CTTTTTTTTTCGTGTTAATA+AGG + chr3_2:85334154-85334173 Msa0433010:intron 20.0%
!!! CAAACAAAGTGTTAAAAAAA+AGG - chr3_2:85334561-85334580 None:intergenic 20.0%
!!! CTTTTTTTTAACACTTTGTT+TGG + chr3_2:85334559-85334578 Msa0433010:intron 20.0%
!! ATTGTAGGATAAAGAATAAA+AGG - chr3_2:85335025-85335044 None:intergenic 20.0%
!! GCTAATATACAAACTTATTA+TGG + chr3_2:85335203-85335222 Msa0433010:intron 20.0%
!! AGAGATTGAAAATAAATTTC+TGG + chr3_2:85335295-85335314 Msa0433010:intron 20.0%
!! GAGATTGAAAATAAATTTCT+GGG + chr3_2:85335296-85335315 Msa0433010:intron 20.0%
!! AGATTGAAAATAAATTTCTG+GGG + chr3_2:85335297-85335316 Msa0433010:intron 20.0%
!! TATCATTAGGTATACATTAT+TGG + chr3_2:85335323-85335342 Msa0433010:intron 20.0%
!!! AAAAAAACTAATGGATGTTA+TGG - chr3_2:85335455-85335474 None:intergenic 20.0%
!!! TAACATCCATTAGTTTTTTT+AGG + chr3_2:85335455-85335474 Msa0433010:intron 20.0%
!! GATATTCCTAAAAAAACTAA+TGG - chr3_2:85335464-85335483 None:intergenic 20.0%
!!! AATTTTATCATCATGAGTTT+GGG + chr3_2:85335754-85335773 Msa0433010:three_prime_UTR 20.0%
!!! TAAACTTTTTCAAGTGAAAT+GGG - chr3_2:85335872-85335891 None:intergenic 20.0%
! AGAAGTGAATAAACGAATTA+GGG - chr3_2:85333110-85333129 None:intergenic 25.0%
! CGGAAATTAACTAAAAAGAT+TGG - chr3_2:85333346-85333365 None:intergenic 25.0%
! ATGCATTTAAATAAATGACG+AGG - chr3_2:85333761-85333780 None:intergenic 25.0%
! TAAATTCATAGTTGAAGTGA+AGG - chr3_2:85334021-85334040 None:intergenic 25.0%
! GTAATATTGGCACAAAAAAA+AGG - chr3_2:85334071-85334090 None:intergenic 25.0%
! TCATTATCATAATATTGTGC+TGG + chr3_2:85334118-85334137 Msa0433010:intron 25.0%
! CAAAATACTGATAAATGCAA+GGG - chr3_2:85334203-85334222 None:intergenic 25.0%
!! CTTGCATTTATCAGTATTTT+GGG + chr3_2:85334202-85334221 Msa0433010:intron 25.0%
!!! TGTCTTTTTGATGACATAAT+AGG + chr3_2:85334348-85334367 Msa0433010:intron 25.0%
! TACTGCAATATAAAGATAAC+AGG - chr3_2:85334427-85334446 None:intergenic 25.0%
!! AACATTTTTGTATGTTAGAC+GGG - chr3_2:85334489-85334508 None:intergenic 25.0%
!! GAACATTTTTGTATGTTAGA+CGG - chr3_2:85334490-85334509 None:intergenic 25.0%
! GTGTTAAAAAAAAGGTTTCA+AGG - chr3_2:85334553-85334572 None:intergenic 25.0%
! ATCCTACAATCATTCATTAT+AGG + chr3_2:85335035-85335054 Msa0433010:intron 25.0%
! TAATATGTTCATTGTAGCAT+TGG + chr3_2:85335063-85335082 Msa0433010:intron 25.0%
!! GATTGAAAATAAATTTCTGG+GGG + chr3_2:85335298-85335317 Msa0433010:intron 25.0%
!! GAATTTTATCATCATGAGTT+TGG + chr3_2:85335753-85335772 Msa0433010:three_prime_UTR 25.0%
!!! GTAAACTTTTTCAAGTGAAA+TGG - chr3_2:85335873-85335892 None:intergenic 25.0%
GAGAAGTGAATAAACGAATT+AGG - chr3_2:85333111-85333130 None:intergenic 30.0%
!! ATTGATTTGGAAGTGAACTT+CGG + chr3_2:85333410-85333429 Msa0433010:intron 30.0%
TGATTGTAAACAGCTACAAT+TGG + chr3_2:85333619-85333638 Msa0433010:intron 30.0%
!! TGTGTTCTCAAGTTTTTAAG+TGG + chr3_2:85333899-85333918 Msa0433010:intron 30.0%
! CATGGATCATTTGATTTAAC+AGG - chr3_2:85333934-85333953 None:intergenic 30.0%
GTTAAATCAAATGATCCATG+TGG + chr3_2:85333934-85333953 Msa0433010:intron 30.0%
TAAGTGATATACAAACCACA+TGG - chr3_2:85333952-85333971 None:intergenic 30.0%
!!! TTTTTTGTGCCAATATTACG+TGG + chr3_2:85334072-85334091 Msa0433010:intron 30.0%
!!! TTTTTTCGTGTTAATAAGGG+TGG + chr3_2:85334158-85334177 Msa0433010:intron 30.0%
CCAAAATACTGATAAATGCA+AGG - chr3_2:85334204-85334223 None:intergenic 30.0%
! CCTTGCATTTATCAGTATTT+TGG + chr3_2:85334201-85334220 Msa0433010:intron 30.0%
TGTAGTGGTTATATACATGT+CGG + chr3_2:85334249-85334268 Msa0433010:intron 30.0%
AGCAAGTAAAACAAGAACAA+AGG - chr3_2:85334303-85334322 None:intergenic 30.0%
!! CGTAGTCATTTTATATGTGT+CGG + chr3_2:85334325-85334344 Msa0433010:intron 30.0%
TCTTTATATTGCAGTATCCA+CGG + chr3_2:85334431-85334450 Msa0433010:intron 30.0%
ATAAAGCAACATAAGGAACA+TGG - chr3_2:85334595-85334614 None:intergenic 30.0%
!! ATGTTCCTTATGTTGCTTTA+TGG + chr3_2:85334594-85334613 Msa0433010:intron 30.0%
!! CTATTTGGAACTGATTTTCA+GGG + chr3_2:85334624-85334643 Msa0433010:intron 30.0%
ATTCTACAAAGTGCAAGTTA+GGG - chr3_2:85334929-85334948 None:intergenic 30.0%
AAATCAATTTGAACTTCTGG+TGG - chr3_2:85334966-85334985 None:intergenic 30.0%
ACCAGAAGTTCAAATTGATT+TGG + chr3_2:85334965-85334984 Msa0433010:CDS 30.0%
TGCCTATAATGAATGATTGT+AGG - chr3_2:85335040-85335059 None:intergenic 30.0%
! GCTATAATATTTTGTGCTTG+AGG + chr3_2:85335691-85335710 Msa0433010:three_prime_UTR 30.0%
! GGTTGAACTTTTAGCATTTT+TGG + chr3_2:85335712-85335731 Msa0433010:three_prime_UTR 30.0%
!!! AGCATTTTTGGTTACAGATT+TGG + chr3_2:85335724-85335743 Msa0433010:three_prime_UTR 30.0%
!!! GCATTTTTGGTTACAGATTT+GGG + chr3_2:85335725-85335744 Msa0433010:three_prime_UTR 30.0%
TTATGTAAACCCTCACTTAA+TGG + chr3_2:85335788-85335807 Msa0433010:three_prime_UTR 30.0%
CTAGTAAAATGTGTTAGAAC+AGG - chr3_2:85335895-85335914 None:intergenic 30.0%
TGCAATCTGTCTCTTCAATT+GGG - chr3_2:85333015-85333034 None:intergenic 35.0%
TTGCAATCTGTCTCTTCAAT+TGG - chr3_2:85333016-85333035 None:intergenic 35.0%
CAAAAGGAATAGCGTGAAAT+CGG - chr3_2:85333206-85333225 None:intergenic 35.0%
ATTGTTAATCGAACGGCAAA+AGG - chr3_2:85333222-85333241 None:intergenic 35.0%
!! TAATCAACGGTTTGCTGATT+GGG - chr3_2:85333279-85333298 None:intergenic 35.0%
CATAGAATCAGTGATTCGTT+AGG + chr3_2:85333378-85333397 Msa0433010:intron 35.0%
! TAGGATCTGGCATATTGATT+TGG + chr3_2:85333397-85333416 Msa0433010:intron 35.0%
GAAAGACAACATTTGTGAAG+AGG + chr3_2:85333462-85333481 Msa0433010:CDS 35.0%
CAGTTTGTGAAGAAATCCAA+TGG - chr3_2:85333658-85333677 None:intergenic 35.0%
TTGGATTTCTTCACAAACTG+CGG + chr3_2:85333658-85333677 Msa0433010:CDS 35.0%
!!! CTGTGATCTCTAGTTTTTGA+TGG + chr3_2:85333781-85333800 Msa0433010:intron 35.0%
AACAGGGAATTTGGTTAAAC+AGG - chr3_2:85333810-85333829 None:intergenic 35.0%
GTTTAACCAAATTCCCTGTT+CGG + chr3_2:85333810-85333829 Msa0433010:intron 35.0%
AGTGTTACATAAACCGAACA+GGG - chr3_2:85333826-85333845 None:intergenic 35.0%
AAGTGTTACATAAACCGAAC+AGG - chr3_2:85333827-85333846 None:intergenic 35.0%
!! TCAATTGCTTGCTTTTGTAG+TGG + chr3_2:85334234-85334253 Msa0433010:intron 35.0%
GACATAATAGGATTCTCCTT+CGG + chr3_2:85334360-85334379 Msa0433010:intron 35.0%
CTTTATATTGCAGTATCCAC+GGG + chr3_2:85334432-85334451 Msa0433010:intron 35.0%
CATACAAAAATGTTCCTACC+TGG + chr3_2:85334495-85334514 Msa0433010:CDS 35.0%
TTACTAACCGACAAAGATCA+CGG - chr3_2:85334523-85334542 None:intergenic 35.0%
AGTGTCCATAAAGCAACATA+AGG - chr3_2:85334602-85334621 None:intergenic 35.0%
CTTTATGGACACTTCCTATT+TGG + chr3_2:85334609-85334628 Msa0433010:intron 35.0%
CCTGAAAATCAGTTCCAAAT+AGG - chr3_2:85334626-85334645 None:intergenic 35.0%
!! CCTATTTGGAACTGATTTTC+AGG + chr3_2:85334623-85334642 Msa0433010:intron 35.0%
AACATCCCAATTGTTCTTTG+CGG + chr3_2:85334655-85334674 Msa0433010:CDS 35.0%
TGTTACCGCAAAGAACAATT+GGG - chr3_2:85334663-85334682 None:intergenic 35.0%
TTGTTACCGCAAAGAACAAT+TGG - chr3_2:85334664-85334683 None:intergenic 35.0%
AATTGTTCTTTGCGGTAACA+AGG + chr3_2:85334663-85334682 Msa0433010:CDS 35.0%
! ACTGCAAATTTTTCTTCCTG+TGG - chr3_2:85334732-85334751 None:intergenic 35.0%
!! TTTCTAGTTTCTACACAGTC+AGG + chr3_2:85334837-85334856 Msa0433010:intron 35.0%
GATTCTACAAAGTGCAAGTT+AGG - chr3_2:85334930-85334949 None:intergenic 35.0%
GCCAAATCAATTTGAACTTC+TGG - chr3_2:85334969-85334988 None:intergenic 35.0%
CAACAAAGGTAAGCTATCTT+TGG + chr3_2:85334993-85335012 Msa0433010:intron 35.0%
! ATTGTAGCATTGGAAAGTAG+TGG + chr3_2:85335073-85335092 Msa0433010:intron 35.0%
!! GGTTGTGAGACTTTGATTTT+AGG - chr3_2:85335118-85335137 None:intergenic 35.0%
TCACAACCATCATTGATATG+AGG + chr3_2:85335130-85335149 Msa0433010:intron 35.0%
CACAACCATCATTGATATGA+GGG + chr3_2:85335131-85335150 Msa0433010:intron 35.0%
CATAACCCTCATATCAATGA+TGG - chr3_2:85335139-85335158 None:intergenic 35.0%
ACACAGCATTTAAACTCATG+AGG - chr3_2:85335237-85335256 None:intergenic 35.0%
! ACACGTGGATTAATGTTCAA+CGG - chr3_2:85335361-85335380 None:intergenic 35.0%
TTGATACGTCCATTAAGTGA+GGG - chr3_2:85335800-85335819 None:intergenic 35.0%
ATTGATACGTCCATTAAGTG+AGG - chr3_2:85335801-85335820 None:intergenic 35.0%
!!! ACTTTTTCAAGTGAAATGGG+TGG - chr3_2:85335869-85335888 None:intergenic 35.0%
TCTTCTTTCGAACCACACTA+TGG + chr3_2:85333159-85333178 Msa0433010:exon 40.0%
! TCATCGTGTTTTTCTTCTGC+AGG + chr3_2:85333248-85333267 Msa0433010:intron 40.0%
!! GTAATCAACGGTTTGCTGAT+TGG - chr3_2:85333280-85333299 None:intergenic 40.0%
ATCAGTGATTCGTTAGGATC+TGG + chr3_2:85333384-85333403 Msa0433010:intron 40.0%
!! TTTGTGAAGAGGCATCTTAC+TGG + chr3_2:85333473-85333492 Msa0433010:CDS 40.0%
GTAAACAGCTACAATTGGTG+TGG + chr3_2:85333624-85333643 Msa0433010:intron 40.0%
CATAAACCGAACAGGGAATT+TGG - chr3_2:85333819-85333838 None:intergenic 40.0%
TGCAACACACCACGTAATAT+TGG - chr3_2:85334084-85334103 None:intergenic 40.0%
!! TAATAAGGGTGGCTGTTTCT+TGG + chr3_2:85334169-85334188 Msa0433010:intron 40.0%
TTATAATAGCACATTGCCCG+TGG - chr3_2:85334451-85334470 None:intergenic 40.0%
!! ACAAGGTTGATGTGAAGAAC+AGG + chr3_2:85334680-85334699 Msa0433010:CDS 40.0%
TTCCTAGCAAGGTAAAGGAA+TGG - chr3_2:85334796-85334815 None:intergenic 40.0%
CTTTGTAGAATCTCCTGCAT+TGG + chr3_2:85334938-85334957 Msa0433010:CDS 40.0%
!! TTGATTTGGCTGCACAACAA+AGG + chr3_2:85334979-85334998 Msa0433010:CDS 40.0%
! ATTTCTGGGGGCATATCATT+AGG + chr3_2:85335310-85335329 Msa0433010:intron 40.0%
AGGGATGATGAAAAAACACG+TGG - chr3_2:85335376-85335395 None:intergenic 40.0%
GTACTGCATATTCCTAAACG+AGG - chr3_2:85335417-85335436 None:intergenic 40.0%
ATCATCATCGTCATCAGGAA+GGG - chr3_2:85335608-85335627 None:intergenic 40.0%
AATGCATCATCATCGTCATC+AGG - chr3_2:85335613-85335632 None:intergenic 40.0%
! CGATGATGATGCATTTGAGT+AGG + chr3_2:85335618-85335637 Msa0433010:CDS 40.0%
! TTGAAAGCGTGAAACTGACG+CGG - chr3_2:85333061-85333080 None:intergenic 45.0%
! ACTGAGAGCATACCATAGTG+TGG - chr3_2:85333174-85333193 None:intergenic 45.0%
CTTGAAGCTCGGGTAATCAA+CGG - chr3_2:85333292-85333311 None:intergenic 45.0%
ACGATGACAAGCTTGAAGCT+CGG - chr3_2:85333303-85333322 None:intergenic 45.0%
AGCTTCAAGCTTGTCATCGT+CGG + chr3_2:85333303-85333322 Msa0433010:CDS 45.0%
CAAGATTCGCTTCTACTGCT+GGG + chr3_2:85333681-85333700 Msa0433010:CDS 45.0%
ACTGCTGGACAAGAGAAGTT+TGG + chr3_2:85333706-85333725 Msa0433010:CDS 45.0%
! AAGTTTGGTGGTCTCAGAGA+TGG + chr3_2:85333721-85333740 Msa0433010:CDS 45.0%
ACAAGCACAAACAATGCCGA+AGG - chr3_2:85334379-85334398 None:intergenic 45.0%
TGTGAAGAACAGGCAAGTGA+AGG + chr3_2:85334690-85334709 Msa0433010:CDS 45.0%
! CAAAGCAGGTTACTTTCCAC+AGG + chr3_2:85334713-85334732 Msa0433010:CDS 45.0%
AGCCATTCCTTTACCTTGCT+AGG + chr3_2:85334791-85334810 Msa0433010:CDS 45.0%
CAAGCTTCCTAGCAAGGTAA+AGG - chr3_2:85334801-85334820 None:intergenic 45.0%
AACGAGGCGGATAAAAGGAA+GGG - chr3_2:85335401-85335420 None:intergenic 45.0%
AAACGAGGCGGATAAAAGGA+AGG - chr3_2:85335402-85335421 None:intergenic 45.0%
! TCCTTTTATCCGCCTCGTTT+AGG + chr3_2:85335402-85335421 Msa0433010:intron 45.0%
TCCTAAACGAGGCGGATAAA+AGG - chr3_2:85335406-85335425 None:intergenic 45.0%
CTGCATATTCCTAAACGAGG+CGG - chr3_2:85335414-85335433 None:intergenic 45.0%
TCATCATCGTCATCAGGAAG+GGG - chr3_2:85335607-85335626 None:intergenic 45.0%
CATCATCATCGTCATCAGGA+AGG - chr3_2:85335609-85335628 None:intergenic 45.0%
TGCTATCCTTTGTCTCGAGT+TGG + chr3_2:85335652-85335671 Msa0433010:three_prime_UTR 45.0%
ACGTATCAATACTCTGCTGC+AGG + chr3_2:85335811-85335830 Msa0433010:three_prime_UTR 45.0%
CGATGACAAGCTTGAAGCTC+GGG - chr3_2:85333302-85333321 None:intergenic 50.0%
! TGGTGTGGAAGTGCATCCAT+TGG + chr3_2:85333639-85333658 Msa0433010:CDS 50.0%
GCAAGATTCGCTTCTACTGC+TGG + chr3_2:85333680-85333699 Msa0433010:CDS 50.0%
TTCTACTGCTGGGATACTGC+TGG + chr3_2:85333691-85333710 Msa0433010:CDS 50.0%
GCTGGACAAGAGAAGTTTGG+TGG + chr3_2:85333709-85333728 Msa0433010:CDS 50.0%
TACCTTGCTAGGAAGCTTGC+AGG + chr3_2:85334802-85334821 Msa0433010:CDS 50.0%
ACCTTGCTAGGAAGCTTGCA+GGG + chr3_2:85334803-85334822 Msa0433010:CDS 50.0%
GCGGATAAAAGGAAGGGACA+GGG - chr3_2:85335395-85335414 None:intergenic 50.0%
! GTCATCAGGAAGGGGTTGAT+TGG - chr3_2:85335599-85335618 None:intergenic 50.0%
ACAAAGGATAGCAGTCGCAC+GGG - chr3_2:85335645-85335664 None:intergenic 50.0%
GACAAAGGATAGCAGTCGCA+CGG - chr3_2:85335646-85335665 None:intergenic 50.0%
ACAGCACCAACTCGAGACAA+AGG - chr3_2:85335661-85335680 None:intergenic 50.0%
GACGCGGCTCTTCTGTCTTT+CGG - chr3_2:85333045-85333064 None:intergenic 55.0%
AAGCTTGTCATCGTCGGCGA+TGG + chr3_2:85333309-85333328 Msa0433010:CDS 55.0%
! CAAAGATCACGGTGCCAGGT+AGG - chr3_2:85334512-85334531 None:intergenic 55.0%
CCTGGCACCGTGATCTTTGT+CGG + chr3_2:85334513-85334532 Msa0433010:CDS 55.0%
CAGGCAAGTGAAGGCAAAGC+AGG + chr3_2:85334699-85334718 Msa0433010:CDS 55.0%
ACCCTGCAAGCTTCCTAGCA+AGG - chr3_2:85334807-85334826 None:intergenic 55.0%
TGCTAGGAAGCTTGCAGGGT+AGG + chr3_2:85334807-85334826 Msa0433010:intron 55.0%
ACTTCTGGTGGAGCCAATGC+AGG - chr3_2:85334954-85334973 None:intergenic 55.0%
GGCGGATAAAAGGAAGGGAC+AGG - chr3_2:85335396-85335415 None:intergenic 55.0%
! GAAGGGGTTGATTGGCAGCT+TGG - chr3_2:85335591-85335610 None:intergenic 55.0%
CTTGTCATCGTCGGCGATGG+CGG + chr3_2:85333312-85333331 Msa0433010:CDS 60.0%
CCGACAAAGATCACGGTGCC+AGG - chr3_2:85334516-85334535 None:intergenic 60.0%
! ATCGTCGGCGATGGCGGCAC+TGG + chr3_2:85333318-85333337 Msa0433010:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 85333012 85335949 85333012 ID=Msa0433010;Name=Msa0433010
chr3_2 mRNA 85333012 85335949 85333012 ID=Msa0433010-mRNA-1;Parent=Msa0433010;Name=Msa0433010-mRNA-1;_AED=0.17;_eAED=0.17;_QI=166|1|1|1|0.87|0.77|9|286|221
chr3_2 exon 85333012 85333180 85333012 ID=Msa0433010-mRNA-1:exon:24370;Parent=Msa0433010-mRNA-1
chr3_2 exon 85333270 85333339 85333270 ID=Msa0433010-mRNA-1:exon:24371;Parent=Msa0433010-mRNA-1
chr3_2 exon 85333462 85333518 85333462 ID=Msa0433010-mRNA-1:exon:24372;Parent=Msa0433010-mRNA-1
chr3_2 exon 85333632 85333749 85333632 ID=Msa0433010-mRNA-1:exon:24373;Parent=Msa0433010-mRNA-1
chr3_2 exon 85334445 85334534 85334445 ID=Msa0433010-mRNA-1:exon:24374;Parent=Msa0433010-mRNA-1
chr3_2 exon 85334645 85334824 85334645 ID=Msa0433010-mRNA-1:exon:24375;Parent=Msa0433010-mRNA-1
chr3_2 exon 85334923 85335000 85334923 ID=Msa0433010-mRNA-1:exon:24376;Parent=Msa0433010-mRNA-1
chr3_2 exon 85335570 85335880 85335570 ID=Msa0433010-mRNA-1:exon:24377;Parent=Msa0433010-mRNA-1
chr3_2 exon 85335905 85335949 85335905 ID=Msa0433010-mRNA-1:exon:24378;Parent=Msa0433010-mRNA-1
chr3_2 five_prime_UTR 85333012 85333177 85333012 ID=Msa0433010-mRNA-1:five_prime_utr;Parent=Msa0433010-mRNA-1
chr3_2 CDS 85333178 85333180 85333178 ID=Msa0433010-mRNA-1:cds;Parent=Msa0433010-mRNA-1
chr3_2 CDS 85333270 85333339 85333270 ID=Msa0433010-mRNA-1:cds;Parent=Msa0433010-mRNA-1
chr3_2 CDS 85333462 85333518 85333462 ID=Msa0433010-mRNA-1:cds;Parent=Msa0433010-mRNA-1
chr3_2 CDS 85333632 85333749 85333632 ID=Msa0433010-mRNA-1:cds;Parent=Msa0433010-mRNA-1
chr3_2 CDS 85334445 85334534 85334445 ID=Msa0433010-mRNA-1:cds;Parent=Msa0433010-mRNA-1
chr3_2 CDS 85334645 85334824 85334645 ID=Msa0433010-mRNA-1:cds;Parent=Msa0433010-mRNA-1
chr3_2 CDS 85334923 85335000 85334923 ID=Msa0433010-mRNA-1:cds;Parent=Msa0433010-mRNA-1
chr3_2 CDS 85335570 85335639 85335570 ID=Msa0433010-mRNA-1:cds;Parent=Msa0433010-mRNA-1
chr3_2 three_prime_UTR 85335640 85335880 85335640 ID=Msa0433010-mRNA-1:three_prime_utr;Parent=Msa0433010-mRNA-1
chr3_2 three_prime_UTR 85335905 85335949 85335905 ID=Msa0433010-mRNA-1:three_prime_utr;Parent=Msa0433010-mRNA-1
Gene Sequence

>Msa0433010

ATGGCTTTGCCCAATCAGCAAACCGTTGATTACCCGAGCTTCAAGCTTGTCATCGTCGGCGATGGCGGCACTGGAAAGACAACATTTGTGAAGAGGCATCTTACTGGAGAATTTGAGAAGAAATATGAACCTACAATTGGTGTGGAAGTGCATCCATTGGATTTCTTCACAAACTGCGGCAAGATTCGCTTCTACTGCTGGGATACTGCTGGACAAGAGAAGTTTGGTGGTCTCAGAGATGGATACTATATCCACGGGCAATGTGCTATTATAATGTTTGATGTTACTGCCCGTCTAACATACAAAAATGTTCCTACCTGGCACCGTGATCTTTGTCGGGTCTGTGAAAACATCCCAATTGTTCTTTGCGGTAACAAGGTTGATGTGAAGAACAGGCAAGTGAAGGCAAAGCAGGTTACTTTCCACAGGAAGAAAAATTTGCAGTACTATGAGATTTCAGCTAAGAGCAACTATAACTTTGAGAAGCCATTCCTTTACCTTGCTAGGAAGCTTGCAGGTGACCCTAACTTGCACTTTGTAGAATCTCCTGCATTGGCTCCACCAGAAGTTCAAATTGATTTGGCTGCACAACAAAGACATGAGAATGAGCTTCTCCAAGCTGCCAATCAACCCCTTCCTGATGACGATGATGATGCATTTGAGTAG
Protein sequence

>Msa0433010

MALPNQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQIDLAAQQRHENELLQAANQPLPDDDDDAFE*