AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1335440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1335440 MtrunA17_Chr3g0138101 99.548 221 1 0 1 221 1 221 4.20e-168 461
Msa1335440 MtrunA17_Chr3g0138121 98.643 221 3 0 1 221 1 221 6.05e-167 458
Msa1335440 MtrunA17_Chr3g0138111 98.643 221 3 0 1 221 1 221 1.59e-166 457
Msa1335440 MtrunA17_Chr4g0063211 72.993 137 25 1 85 221 7 131 1.18e-66 201
Msa1335440 MtrunA17_Chr4g0055081 30.052 193 128 4 14 203 10 198 3.80e-27 103
Msa1335440 MtrunA17_Chr4g0046261 30.526 190 126 4 14 197 14 203 2.98e-26 100
Msa1335440 MtrunA17_Chr5g0400131 31.977 172 106 3 14 178 10 177 3.73e-26 100
Msa1335440 MtrunA17_Chr3g0109721 33.526 173 109 4 15 184 12 181 7.23e-26 99.8
Msa1335440 MtrunA17_Chr8g0390561 34.161 161 103 3 15 172 11 171 8.08e-26 99.4
Msa1335440 MtrunA17_Chr2g0283131 30.526 190 126 4 14 197 14 203 1.33e-25 99.4
Msa1335440 MtrunA17_Chr8g0383261 30.723 166 105 3 14 169 13 178 1.41e-25 99.0
Msa1335440 MtrunA17_Chr1g0201121 27.749 191 125 4 14 191 9 199 1.63e-25 99.0
Msa1335440 MtrunA17_Chr8g0349711 32.716 162 106 3 13 171 6 167 3.20e-25 98.2
Msa1335440 MtrunA17_Chr1g0159481 37.647 170 98 6 14 177 18 185 4.85e-25 97.8
Msa1335440 MtrunA17_Chr4g0032201 31.844 179 114 3 14 189 13 186 5.68e-25 97.8
Msa1335440 MtrunA17_Chr8g0349681 33.951 162 104 3 13 171 175 336 6.09e-25 102
Msa1335440 MtrunA17_Chr8g0349681 32.515 163 107 3 13 172 13 175 2.41e-22 94.7
Msa1335440 MtrunA17_Chr8g0349681 32.298 161 106 2 14 171 385 545 5.72e-22 93.6
Msa1335440 MtrunA17_Chr8g0381841 31.915 188 119 5 7 191 12 193 8.84e-25 97.4
Msa1335440 MtrunA17_Chr8g0384311 34.637 179 110 5 4 177 5 181 1.04e-24 96.7
Msa1335440 MtrunA17_Chr3g0123971 32.143 168 111 3 14 178 10 177 1.05e-24 96.7
Msa1335440 MtrunA17_Chr4g0040781 30.000 190 127 4 14 197 14 203 1.24e-24 96.7
Msa1335440 MtrunA17_Chr3g0112681 34.731 167 105 4 1 164 1 166 1.34e-24 96.7
Msa1335440 MtrunA17_Chr1g0182321 29.381 194 128 5 2 190 3 192 1.43e-24 96.7
Msa1335440 MtrunA17_Chr5g0433561 32.948 173 110 4 15 184 12 181 1.53e-24 96.3
Msa1335440 MtrunA17_Chr6g0482721 34.483 174 110 4 1 171 1 173 1.74e-24 96.3
Msa1335440 MtrunA17_Chr4g0055651 30.435 184 119 4 14 192 9 188 2.20e-24 95.9
Msa1335440 MtrunA17_Chr1g0182301 28.866 194 129 5 2 190 3 192 4.95e-24 95.1
Msa1335440 MtrunA17_Chr2g0319951 33.117 154 100 2 14 164 14 167 5.62e-24 95.1
Msa1335440 MtrunA17_Chr2g0325091 32.903 155 101 2 14 165 12 166 5.67e-24 95.1
Msa1335440 MtrunA17_Chr1g0212521 30.247 162 109 3 14 171 16 177 6.37e-24 94.7
Msa1335440 MtrunA17_Chr4g0030911 30.247 162 109 3 14 171 16 177 7.69e-24 94.7
Msa1335440 MtrunA17_Chr8g0382221 32.143 168 111 3 14 178 10 177 8.58e-24 94.4
Msa1335440 MtrunA17_Chr2g0276941 34.416 154 98 2 14 164 16 169 9.33e-24 94.7
Msa1335440 MtrunA17_Chr6g0466301 27.513 189 124 4 14 189 9 197 9.42e-24 94.4
Msa1335440 MtrunA17_Chr6g0465491 27.513 189 124 4 14 189 9 197 9.42e-24 94.4
Msa1335440 MtrunA17_Chr3g0123821 29.126 206 129 5 7 198 11 213 1.20e-23 94.4
Msa1335440 MtrunA17_Chr7g0263071 30.247 162 109 3 14 171 16 177 1.32e-23 94.0
Msa1335440 MtrunA17_Chr2g0315551 32.468 154 101 2 14 164 14 167 1.58e-23 94.0
Msa1335440 MtrunA17_Chr6g0458081 32.298 161 106 2 14 171 7 167 2.66e-23 93.2
Msa1335440 MtrunA17_Chr8g0351861 31.765 170 112 4 5 171 9 177 3.51e-23 93.2
Msa1335440 MtrunA17_Chr8g0335471 32.447 188 111 6 14 192 9 189 6.13e-23 92.0
Msa1335440 MtrunA17_Chr7g0275991 33.333 168 108 3 7 171 15 181 7.87e-23 92.4
Msa1335440 MtrunA17_Chr7g0265001 35.088 171 104 5 13 177 16 185 8.05e-23 92.0
Msa1335440 MtrunA17_Chr7g0250531 30.108 186 117 4 14 192 9 188 1.04e-22 91.3
Msa1335440 MtrunA17_Chr4g0034871 31.214 173 95 6 14 169 9 174 1.10e-22 91.3
Msa1335440 MtrunA17_Chr3g0130271 34.973 183 111 6 1 177 1 181 1.59e-22 90.9
Msa1335440 MtrunA17_Chr7g0214241 31.056 161 108 3 14 171 16 176 1.86e-22 91.3
Msa1335440 MtrunA17_Chr2g0331691 31.677 161 100 3 14 164 11 171 2.83e-22 90.5
Msa1335440 MtrunA17_Chr8g0392931 33.121 157 102 2 14 167 15 171 3.15e-22 90.5
Msa1335440 MtrunA17_Chr2g0327911 28.834 163 111 2 14 171 16 178 6.30e-22 89.7
Msa1335440 MtrunA17_Chr8g0346421 33.136 169 109 4 6 171 20 187 8.69e-22 89.7
Msa1335440 MtrunA17_Chr4g0004661 31.792 173 94 6 14 169 65 230 9.01e-22 90.1
Msa1335440 MtrunA17_Chr1g0180291 32.749 171 107 6 13 177 14 182 1.11e-21 89.0
Msa1335440 MtrunA17_Chr1g0174281 31.098 164 109 3 7 167 10 172 2.34e-21 88.2
Msa1335440 MtrunA17_Chr7g0231131 32.716 162 105 4 9 167 12 172 2.72e-21 88.2
Msa1335440 MtrunA17_Chr2g0290891 31.646 158 105 2 14 168 7 164 3.13e-21 87.8
Msa1335440 MtrunA17_Chr1g0189361 30.114 176 117 5 1 171 1 175 1.19e-20 86.3
Msa1335440 MtrunA17_Chr1g0199331 29.193 161 110 3 15 171 33 193 4.62e-20 84.3
Msa1335440 MtrunA17_Chr7g0234201 28.571 161 112 3 14 171 14 174 4.73e-20 84.7
Msa1335440 MtrunA17_Chr2g0276931 30.061 163 107 4 14 171 10 170 1.07e-19 83.6
Msa1335440 MtrunA17_Chr1g0197331 29.442 197 123 7 10 199 13 200 7.80e-19 81.3
Msa1335440 MtrunA17_Chr8g0371501 28.485 165 103 4 14 164 7 170 3.92e-14 68.6
Msa1335440 MtrunA17_Chr6g0486041 33.051 118 77 2 14 130 7 123 5.34e-14 68.2
Msa1335440 MtrunA17_Chr5g0405791 28.485 165 103 4 14 164 7 170 9.04e-14 67.4
Msa1335440 MtrunA17_Chr4g0046211 30.252 119 81 2 14 131 7 124 9.33e-14 66.2
Msa1335440 MtrunA17_Chr6g0466161 27.815 151 99 3 29 169 6 156 1.60e-13 67.0
Msa1335440 MtrunA17_Chr6g0465471 27.815 151 99 3 29 169 6 156 1.98e-13 66.6
Msa1335440 MtrunA17_Chr5g0443631 26.012 173 121 1 10 175 101 273 1.53e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1335440 AT5G55190.1 96.833 221 7 0 1 221 1 221 7.36e-164 451
Msa1335440 AT5G20010.1 95.475 221 10 0 1 221 1 221 4.71e-161 444
Msa1335440 AT5G20020.1 95.023 221 11 0 1 221 1 221 1.41e-152 422
Msa1335440 AT5G55080.1 70.283 212 58 1 1 212 1 207 1.37e-108 311
Msa1335440 AT4G39890.1 36.158 177 103 4 14 186 10 180 4.84e-28 105
Msa1335440 AT1G07410.1 31.551 187 120 3 14 197 13 194 4.45e-26 100
Msa1335440 AT4G19640.1 33.514 185 116 5 15 195 12 193 5.22e-26 100
Msa1335440 AT2G44610.1 32.143 168 111 3 14 178 10 177 9.85e-26 99.8
Msa1335440 AT4G18430.1 32.298 161 106 3 14 171 14 174 2.46e-25 99.0
Msa1335440 AT5G45130.1 33.140 172 109 4 15 183 12 180 2.54e-25 98.6
Msa1335440 AT5G39620.1 32.335 167 103 3 14 170 6 172 3.30e-25 98.2
Msa1335440 AT5G59150.1 34.731 167 105 3 1 164 1 166 7.86e-25 97.8
Msa1335440 AT5G45750.1 35.256 156 94 3 14 164 14 167 9.91e-25 97.4
Msa1335440 AT4G18800.1 32.597 181 110 4 14 189 14 187 1.54e-24 96.7
Msa1335440 AT1G09630.1 34.132 167 106 3 1 164 1 166 1.66e-24 96.7
Msa1335440 AT2G21880.1 30.909 165 104 3 14 168 10 174 1.94e-24 96.7
Msa1335440 AT5G65270.1 31.217 189 121 5 6 191 11 193 2.87e-24 96.3
Msa1335440 AT5G60860.1 30.000 190 127 4 14 197 14 203 3.17e-24 96.3
Msa1335440 AT2G22290.1 32.515 163 107 3 14 173 10 172 3.48e-24 95.9
Msa1335440 AT1G18200.1 32.919 161 105 3 14 171 14 174 3.50e-24 96.3
Msa1335440 AT4G09720.4 29.240 171 106 3 14 169 9 179 3.89e-24 95.9
Msa1335440 AT4G39990.1 33.333 168 104 4 9 171 14 178 4.70e-24 95.9
Msa1335440 AT3G15060.1 33.766 154 99 3 14 164 14 167 4.73e-24 95.5
Msa1335440 AT4G09720.1 28.916 166 108 3 14 169 9 174 5.19e-24 95.1
Msa1335440 AT3G18820.1 30.882 204 113 7 14 200 9 201 6.21e-24 95.1
Msa1335440 AT1G22740.1 29.630 189 120 6 14 189 9 197 6.38e-24 95.1
Msa1335440 AT3G53610.2 28.729 181 121 4 14 190 16 192 6.76e-24 95.1
Msa1335440 AT3G53610.3 28.729 181 121 4 14 190 16 192 6.76e-24 95.1
Msa1335440 AT3G53610.1 28.729 181 121 4 14 190 16 192 6.76e-24 95.1
Msa1335440 AT5G59840.1 29.319 191 123 5 14 196 16 202 6.83e-24 95.1
Msa1335440 AT5G10260.1 32.738 168 110 3 14 178 10 177 1.33e-23 94.4
Msa1335440 AT3G46830.1 34.783 161 101 3 7 164 7 166 1.57e-23 94.4
Msa1335440 AT3G46830.2 34.783 161 101 3 7 164 7 166 1.57e-23 94.4
Msa1335440 AT5G45130.2 32.353 170 109 4 17 183 22 188 1.68e-23 94.0
Msa1335440 AT3G46060.2 30.247 162 109 3 14 171 16 177 2.42e-23 93.6
Msa1335440 AT3G46060.3 30.247 162 109 3 14 171 16 177 2.42e-23 93.6
Msa1335440 AT3G46060.1 30.247 162 109 3 14 171 16 177 2.42e-23 93.6
Msa1335440 AT4G09720.3 28.249 177 106 3 14 169 9 185 2.42e-23 94.0
Msa1335440 AT4G17530.1 30.435 184 119 4 14 192 9 188 5.32e-23 92.4
Msa1335440 AT5G03530.2 32.353 170 109 4 13 177 13 181 6.62e-23 92.4
Msa1335440 AT5G03530.1 32.353 170 109 4 13 177 13 181 6.62e-23 92.4
Msa1335440 AT5G47200.1 30.435 184 119 4 14 192 9 188 7.45e-23 92.0
Msa1335440 AT1G73640.1 32.544 169 110 4 6 171 7 174 9.24e-23 92.8
Msa1335440 AT3G12160.1 32.927 164 106 4 7 167 10 172 1.01e-22 92.4
Msa1335440 AT5G47960.1 33.951 162 103 4 7 165 10 170 1.02e-22 92.4
Msa1335440 AT5G64990.1 28.079 203 124 6 14 203 8 201 1.63e-22 91.3
Msa1335440 AT2G33870.1 30.270 185 123 4 14 192 14 198 1.94e-22 91.3
Msa1335440 AT3G16100.1 29.798 198 123 5 14 200 9 201 2.14e-22 90.9
Msa1335440 AT1G28550.1 29.730 185 124 4 14 192 14 198 2.34e-22 91.3
Msa1335440 AT1G06400.1 33.766 154 99 3 14 164 14 167 2.47e-22 91.3
Msa1335440 AT1G02130.1 30.811 185 118 5 14 192 9 189 3.26e-22 90.5
Msa1335440 AT1G49300.2 31.977 172 93 6 14 168 9 173 3.44e-22 90.5
Msa1335440 AT1G49300.1 31.977 172 93 6 14 168 9 173 3.44e-22 90.5
Msa1335440 AT1G52280.1 29.293 198 124 6 14 200 9 201 4.25e-22 90.1
Msa1335440 AT1G43890.3 33.136 169 107 4 14 177 14 181 4.30e-22 90.5
Msa1335440 AT1G43890.1 33.136 169 107 4 14 177 14 181 4.30e-22 90.5
Msa1335440 AT1G43890.2 33.136 169 107 4 14 177 14 181 4.30e-22 90.5
Msa1335440 AT1G16920.1 31.818 154 102 2 14 164 14 167 4.88e-22 90.5
Msa1335440 AT2G43130.1 27.461 193 136 4 14 203 13 204 7.20e-22 89.7
Msa1335440 AT4G35860.1 31.056 161 108 2 14 171 7 167 7.75e-22 89.7
Msa1335440 AT1G01200.1 31.953 169 110 4 7 171 23 190 1.03e-21 90.1
Msa1335440 AT4G17160.1 30.380 158 107 2 14 168 7 164 3.19e-21 87.8
Msa1335440 AT4G17170.1 31.056 161 108 2 14 171 7 167 6.37e-21 87.4
Msa1335440 AT3G07410.1 29.310 174 120 3 14 184 13 186 1.19e-20 86.7
Msa1335440 AT1G05810.1 30.519 154 104 3 14 164 13 166 1.35e-20 86.7
Msa1335440 AT5G47520.1 30.435 161 109 3 14 171 15 175 1.46e-20 86.7
Msa1335440 AT1G05810.2 30.519 154 104 3 14 164 59 212 3.90e-20 86.3
Msa1335440 AT4G19640.2 35.938 128 80 2 15 140 12 139 5.46e-20 83.2
Msa1335440 AT4G19640.3 34.043 141 90 3 15 152 12 152 6.85e-20 84.0
Msa1335440 AT5G64990.2 27.143 210 124 7 14 203 8 208 8.94e-20 84.3
Msa1335440 AT3G54840.1 29.814 161 109 4 15 171 35 195 9.68e-20 84.0
Msa1335440 AT5G03520.1 28.272 191 128 5 4 189 5 191 1.70e-19 83.6
Msa1335440 AT2G31680.1 29.193 161 111 3 14 171 13 173 1.73e-19 83.6
Msa1335440 AT3G11730.1 28.108 185 126 3 14 191 9 193 2.86e-19 82.8
Msa1335440 AT3G54840.3 29.375 160 109 4 16 171 2 161 3.64e-19 81.6
Msa1335440 AT3G09900.1 28.272 191 128 5 4 189 5 191 3.77e-19 82.8
Msa1335440 AT3G54840.2 30.519 154 103 4 15 164 35 188 1.64e-18 80.5
Msa1335440 AT3G09910.3 31.176 170 111 4 13 177 13 181 1.93e-17 77.8
Msa1335440 AT3G09910.1 31.176 170 111 4 13 177 13 181 1.93e-17 77.8
Msa1335440 AT3G09910.2 31.176 170 111 4 13 177 13 181 1.93e-17 77.8
Msa1335440 AT2G21880.4 29.530 149 95 3 30 168 1 149 2.68e-17 77.0
Msa1335440 AT2G21880.3 29.530 149 95 3 30 168 1 149 2.68e-17 77.0
Msa1335440 AT3G09910.5 31.098 164 107 4 13 171 13 175 1.00e-16 75.9
Msa1335440 AT3G09910.4 31.098 164 107 4 13 171 13 175 1.00e-16 75.9
Msa1335440 AT4G09720.6 28.777 139 89 3 41 169 2 140 2.00e-16 74.3
Msa1335440 AT4G09720.5 28.777 139 89 3 41 169 2 140 2.00e-16 74.3
Msa1335440 AT4G09720.2 28.777 139 89 3 41 169 2 140 2.00e-16 74.3
Msa1335440 AT2G21880.2 29.091 165 99 5 14 168 10 166 3.70e-16 74.3
Msa1335440 AT4G35860.2 31.858 113 74 2 62 171 9 121 6.07e-15 70.1
Msa1335440 AT5G03520.2 29.323 133 86 4 61 189 53 181 6.72e-15 70.9
Msa1335440 AT5G59150.2 33.962 106 67 2 62 164 10 115 5.76e-14 67.8
Msa1335440 AT4G35950.1 29.697 165 101 4 14 164 7 170 6.57e-14 68.2
Msa1335440 AT2G17800.2 31.933 119 79 2 14 131 7 124 9.19e-14 67.8
Msa1335440 AT2G17800.1 31.933 119 79 2 14 131 7 124 9.19e-14 67.8
Msa1335440 AT3G51300.1 32.203 118 78 2 15 131 8 124 1.09e-13 67.4
Msa1335440 AT4G28950.1 28.834 163 103 4 14 164 7 168 1.38e-13 67.4
Msa1335440 AT4G35020.4 30.702 114 77 2 15 127 8 120 7.77e-11 58.5
Msa1335440 AT4G35020.5 30.702 114 77 2 15 127 8 120 7.77e-11 58.5

Find 75 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGTAAACCCTCACTTAA+TGG 0.159303 tig0000405:+13399 Msa1335440:three_prime_UTR
GAGAAGTGAATAAACGAATT+AGG 0.189362 tig0000405:-10718 None:intergenic
GCCAAATCAATTTGAACTTC+TGG 0.258290 tig0000405:-12577 None:intergenic
TTGCAATCTGTCTCTTCAAT+TGG 0.267988 tig0000405:-10623 None:intergenic
GATTCTACAAAGTGCAAGTT+AGG 0.300639 tig0000405:-12538 None:intergenic
AGAAGTGAATAAACGAATTA+GGG 0.313376 tig0000405:-10717 None:intergenic
ACCAGAAGTTCAAATTGATT+TGG 0.314372 tig0000405:+12576 Msa1335440:CDS
TTTGTGAAGAGGCATCTTAC+TGG 0.332899 tig0000405:+11083 Msa1335440:CDS
TAATCAACGGTTTGCTGATT+GGG 0.341509 tig0000405:-10882 None:intergenic
TACCTTGCTAGGAAGCTTGC+AGG 0.343483 tig0000405:+12413 Msa1335440:CDS
TGCAATCTGTCTCTTCAATT+GGG 0.359194 tig0000405:-10622 None:intergenic
GACGCGGCTCTTCTGTCTTT+CGG 0.366086 tig0000405:-10652 None:intergenic
GCAAGATTCGCTTCTACTGC+TGG 0.380027 tig0000405:+11290 Msa1335440:CDS
TCTTCTTTCGAACCACACTA+TGG 0.402104 tig0000405:+10765 Msa1335440:exon
TTGTTACCGCAAAGAACAAT+TGG 0.403385 tig0000405:-12272 None:intergenic
AACATCCCAATTGTTCTTTG+CGG 0.404004 tig0000405:+12266 Msa1335440:CDS
GTAATCAACGGTTTGCTGAT+TGG 0.420523 tig0000405:-10883 None:intergenic
GAAGGGGTTGATTGGCAGCT+TGG 0.435439 tig0000405:-13199 None:intergenic
CCTGGCACCGTGATCTTTGT+CGG 0.450109 tig0000405:+12124 Msa1335440:CDS
GTCATCAGGAAGGGGTTGAT+TGG 0.454313 tig0000405:-13207 None:intergenic
CTTTGTAGAATCTCCTGCAT+TGG 0.466850 tig0000405:+12549 Msa1335440:CDS
CAGGCAAGTGAAGGCAAAGC+AGG 0.468849 tig0000405:+12310 Msa1335440:CDS
ACTGCTGGACAAGAGAAGTT+TGG 0.471338 tig0000405:+11316 Msa1335440:CDS
ACAAGGTTGATGTGAAGAAC+AGG 0.475162 tig0000405:+12291 Msa1335440:CDS
CGATGACAAGCTTGAAGCTC+GGG 0.476801 tig0000405:-10905 None:intergenic
TGCTATCCTTTGTCTCGAGT+TGG 0.480872 tig0000405:+13263 Msa1335440:three_prime_UTR
ATCGTCGGCGATGGCGGCAC+TGG 0.486311 tig0000405:+10924 Msa1335440:CDS
CTTGTCATCGTCGGCGATGG+CGG 0.488335 tig0000405:+10918 Msa1335440:CDS
AATGCATCATCATCGTCATC+AGG 0.495924 tig0000405:-13221 None:intergenic
TGATTGTAAACAGCTACAAT+TGG 0.508837 tig0000405:+11229 Msa1335440:intron
CAAGCTTCCTAGCAAGGTAA+AGG 0.519388 tig0000405:-12409 None:intergenic
CTTTATATTGCAGTATCCAC+GGG 0.523043 tig0000405:+12043 Msa1335440:intron
CCGACAAAGATCACGGTGCC+AGG 0.524098 tig0000405:-12124 None:intergenic
AAGCTTGTCATCGTCGGCGA+TGG 0.529447 tig0000405:+10915 Msa1335440:CDS
TCTTTATATTGCAGTATCCA+CGG 0.531789 tig0000405:+12042 Msa1335440:intron
ACTTCTGGTGGAGCCAATGC+AGG 0.537856 tig0000405:-12562 None:intergenic
AGCTTCAAGCTTGTCATCGT+CGG 0.540576 tig0000405:+10909 Msa1335440:CDS
AAGTTTGGTGGTCTCAGAGA+TGG 0.543632 tig0000405:+11331 Msa1335440:CDS
TTCTACTGCTGGGATACTGC+TGG 0.543775 tig0000405:+11301 Msa1335440:CDS
AATTGTTCTTTGCGGTAACA+AGG 0.546634 tig0000405:+12274 Msa1335440:CDS
CAAAGCAGGTTACTTTCCAC+AGG 0.547056 tig0000405:+12324 Msa1335440:CDS
CATCATCATCGTCATCAGGA+AGG 0.549641 tig0000405:-13217 None:intergenic
TGTTACCGCAAAGAACAATT+GGG 0.552031 tig0000405:-12271 None:intergenic
ATCATCATCGTCATCAGGAA+GGG 0.552321 tig0000405:-13216 None:intergenic
AGCCATTCCTTTACCTTGCT+AGG 0.553251 tig0000405:+12402 Msa1335440:CDS
AAATCAATTTGAACTTCTGG+TGG 0.563330 tig0000405:-12574 None:intergenic
CAAGATTCGCTTCTACTGCT+GGG 0.564996 tig0000405:+11291 Msa1335440:CDS
TTGATTTGGCTGCACAACAA+AGG 0.579779 tig0000405:+12590 Msa1335440:CDS
TTGATACGTCCATTAAGTGA+GGG 0.582936 tig0000405:-13408 None:intergenic
GACAAAGGATAGCAGTCGCA+CGG 0.584056 tig0000405:-13254 None:intergenic
ACGTATCAATACTCTGCCGC+AGG 0.584999 tig0000405:+13422 Msa1335440:three_prime_UTR
CTTGAAGCTCGGGTAATCAA+CGG 0.586019 tig0000405:-10895 None:intergenic
ACCCTGCAAGCTTCCTAGCA+AGG 0.586472 tig0000405:-12415 None:intergenic
TTGGATTTCTTCACAAACTG+CGG 0.590990 tig0000405:+11268 Msa1335440:CDS
ACGATGACAAGCTTGAAGCT+CGG 0.595291 tig0000405:-10906 None:intergenic
GAAAGACAACATTTGTGAAG+AGG 0.596908 tig0000405:+11072 Msa1335440:CDS
ATTCTACAAAGTGCAAGTTA+GGG 0.598867 tig0000405:-12537 None:intergenic
ATTGATACGTCCATTAAGTG+AGG 0.602666 tig0000405:-13409 None:intergenic
TGGTGTGGAAGTGCATCCAT+TGG 0.604734 tig0000405:+11249 Msa1335440:CDS
TTCCTAGCAAGGTAAAGGAA+TGG 0.606313 tig0000405:-12404 None:intergenic
CAAAGATCACGGTGCCAGGT+AGG 0.607872 tig0000405:-12120 None:intergenic
ACAAAGGATAGCAGTCGCAC+GGG 0.609636 tig0000405:-13253 None:intergenic
CAGTTTGTGAAGAAATCCAA+TGG 0.610326 tig0000405:-11265 None:intergenic
ACCTTGCTAGGAAGCTTGCA+GGG 0.610380 tig0000405:+12414 Msa1335440:CDS
TGTGAAGAACAGGCAAGTGA+AGG 0.615131 tig0000405:+12301 Msa1335440:CDS
GCTGGACAAGAGAAGTTTGG+TGG 0.616663 tig0000405:+11319 Msa1335440:CDS
TCATCATCGTCATCAGGAAG+GGG 0.618873 tig0000405:-13215 None:intergenic
ACAGCACCAACTCGAGACAA+AGG 0.634897 tig0000405:-13269 None:intergenic
TTATAATAGCACATTGCCCG+TGG 0.636385 tig0000405:-12059 None:intergenic
CGATGATGATGCATTTGAGT+AGG 0.644342 tig0000405:+13229 Msa1335440:CDS
GTAAACAGCTACAATTGGTG+TGG 0.644898 tig0000405:+11234 Msa1335440:intron
TTACTAACCGACAAAGATCA+CGG 0.655820 tig0000405:-12131 None:intergenic
ACAAACAAAATCAATGCCTG+CGG 0.692018 tig0000405:-13438 None:intergenic
ACTGAGAGCATACCATAGTG+TGG 0.696735 tig0000405:-10777 None:intergenic
TTGAAAGCGTGAAACTGACG+CGG 0.697502 tig0000405:-10668 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAACTAAAAAGATAAAT+TGG - tig0000405:10952-10971 None:intergenic 10.0%
!!! GTAAGATTCATTTTAATTTA+GGG + tig0000405:11129-11148 Msa1335440:intron 15.0%
!! AAAAAAAAAGTCTACATTTA+TGG - tig0000405:11755-11774 None:intergenic 15.0%
!!! TTTTTTTTTCGTGTTAATAA+GGG + tig0000405:11766-11785 Msa1335440:intron 15.0%
!!! ATTTTGAATTTCTCATTTAA+TGG + tig0000405:13147-13166 Msa1335440:intron 15.0%
!!! TTAGGGTTTTTATATTACAA+GGG - tig0000405:10703-10722 None:intergenic 20.0%
!!! ATTAGGGTTTTTATATTACA+AGG - tig0000405:10704-10723 None:intergenic 20.0%
!!! AATTGTTATTGTTAATCGAA+CGG - tig0000405:10835-10854 None:intergenic 20.0%
!! ATTCTATGTTACATATTTCA+CGG - tig0000405:10976-10995 None:intergenic 20.0%
!!! AATAATGATCATTTTCTTAC+AGG + tig0000405:11050-11069 Msa1335440:intron 20.0%
!!! CGTAAGATTCATTTTAATTT+AGG + tig0000405:11128-11147 Msa1335440:intron 20.0%
!! TTAACAAGCATGATTATATA+CGG + tig0000405:11152-11171 Msa1335440:intron 20.0%
!!! CTTTTTTTTTCGTGTTAATA+AGG + tig0000405:11765-11784 Msa1335440:intron 20.0%
!!! CAAACAAAGTGTTAAAAAAA+AGG - tig0000405:12172-12191 None:intergenic 20.0%
!!! CTTTTTTTTAACACTTTGTT+TGG + tig0000405:12170-12189 Msa1335440:intron 20.0%
!! ATTGTAGGATAAAGAATAAA+AGG - tig0000405:12636-12655 None:intergenic 20.0%
!! GCTAATATACAAACTTATTA+TGG + tig0000405:12814-12833 Msa1335440:intron 20.0%
!! AGAGATTGAAAATAAATTTC+TGG + tig0000405:12906-12925 Msa1335440:intron 20.0%
!! GAGATTGAAAATAAATTTCT+GGG + tig0000405:12907-12926 Msa1335440:intron 20.0%
!! AGATTGAAAATAAATTTCTG+GGG + tig0000405:12908-12927 Msa1335440:intron 20.0%
!! TATCATTAGGTATACATTAT+TGG + tig0000405:12934-12953 Msa1335440:intron 20.0%
!!! AAAAAAACTAATGGATGTTA+TGG - tig0000405:13066-13085 None:intergenic 20.0%
!!! TAACATCCATTAGTTTTTTT+AGG + tig0000405:13066-13085 Msa1335440:intron 20.0%
!! GATATTCCTAAAAAAACTAA+TGG - tig0000405:13075-13094 None:intergenic 20.0%
!!! AATTTTATCATCATGAGTTT+GGG + tig0000405:13365-13384 Msa1335440:three_prime_UTR 20.0%
!!! TAAACTTTTTCAAGTGAAAT+GGG - tig0000405:13483-13502 None:intergenic 20.0%
! AGAAGTGAATAAACGAATTA+GGG - tig0000405:10720-10739 None:intergenic 25.0%
! ATGCATTTAAATAAATGACG+AGG - tig0000405:11371-11390 None:intergenic 25.0%
! TAAATTCATAGTTGAAGTGA+AGG - tig0000405:11632-11651 None:intergenic 25.0%
! GTAATATTGGCACAAAAAAA+AGG - tig0000405:11682-11701 None:intergenic 25.0%
! TCATTATCATAATATTGTGC+TGG + tig0000405:11729-11748 Msa1335440:intron 25.0%
! CAAAATACTGATAAATGCAA+GGG - tig0000405:11814-11833 None:intergenic 25.0%
!! CTTGCATTTATCAGTATTTT+GGG + tig0000405:11813-11832 Msa1335440:intron 25.0%
!!! TGTCTTTTTGATGACATAAT+AGG + tig0000405:11959-11978 Msa1335440:intron 25.0%
! TACTGCAATATAAAGATAAC+AGG - tig0000405:12038-12057 None:intergenic 25.0%
!! AACATTTTTGTATGTTAGAC+GGG - tig0000405:12100-12119 None:intergenic 25.0%
!! GAACATTTTTGTATGTTAGA+CGG - tig0000405:12101-12120 None:intergenic 25.0%
! GTGTTAAAAAAAAGGTTTCA+AGG - tig0000405:12164-12183 None:intergenic 25.0%
! ATCCTACAATCATTCATTAT+AGG + tig0000405:12646-12665 Msa1335440:intron 25.0%
! TAATATGTTCATTGTAGCAT+TGG + tig0000405:12674-12693 Msa1335440:intron 25.0%
!! GATTGAAAATAAATTTCTGG+GGG + tig0000405:12909-12928 Msa1335440:intron 25.0%
!! GAATTTTATCATCATGAGTT+TGG + tig0000405:13364-13383 Msa1335440:three_prime_UTR 25.0%
!!! GTAAACTTTTTCAAGTGAAA+TGG - tig0000405:13484-13503 None:intergenic 25.0%
GAGAAGTGAATAAACGAATT+AGG - tig0000405:10721-10740 None:intergenic 30.0%
!! ATTGATTTGGAAGTGAACTT+CGG + tig0000405:11020-11039 Msa1335440:intron 30.0%
TGATTGTAAACAGCTACAAT+TGG + tig0000405:11229-11248 Msa1335440:intron 30.0%
!! TGTGTTCTCAAGTTTTTAAG+TGG + tig0000405:11509-11528 Msa1335440:intron 30.0%
! CATGGATCATTTGATTTAAC+AGG - tig0000405:11544-11563 None:intergenic 30.0%
GTTAAATCAAATGATCCATG+TGG + tig0000405:11544-11563 Msa1335440:intron 30.0%
TAAGTGATATACAAACCACA+TGG - tig0000405:11562-11581 None:intergenic 30.0%
!!! TTTTTTGTGCCAATATTACG+TGG + tig0000405:11683-11702 Msa1335440:intron 30.0%
!!! TTTTTTCGTGTTAATAAGGG+TGG + tig0000405:11769-11788 Msa1335440:intron 30.0%
CCAAAATACTGATAAATGCA+AGG - tig0000405:11815-11834 None:intergenic 30.0%
! CCTTGCATTTATCAGTATTT+TGG + tig0000405:11812-11831 Msa1335440:intron 30.0%
TGTAGTGGTTATATACATGT+CGG + tig0000405:11860-11879 Msa1335440:intron 30.0%
AGCAAGTAAAACAAGAACAA+AGG - tig0000405:11914-11933 None:intergenic 30.0%
!! CGTAGTCATTTTATATGTGT+CGG + tig0000405:11936-11955 Msa1335440:intron 30.0%
TCTTTATATTGCAGTATCCA+CGG + tig0000405:12042-12061 Msa1335440:intron 30.0%
ATAAAGCAACATAAGGAACA+TGG - tig0000405:12206-12225 None:intergenic 30.0%
!! ATGTTCCTTATGTTGCTTTA+TGG + tig0000405:12205-12224 Msa1335440:intron 30.0%
!! CTATTTGGAACTGATTTTCA+GGG + tig0000405:12235-12254 Msa1335440:intron 30.0%
ATTCTACAAAGTGCAAGTTA+GGG - tig0000405:12540-12559 None:intergenic 30.0%
AAATCAATTTGAACTTCTGG+TGG - tig0000405:12577-12596 None:intergenic 30.0%
ACCAGAAGTTCAAATTGATT+TGG + tig0000405:12576-12595 Msa1335440:CDS 30.0%
TGCCTATAATGAATGATTGT+AGG - tig0000405:12651-12670 None:intergenic 30.0%
! GCTATAATATTTTGTGCTTG+AGG + tig0000405:13302-13321 Msa1335440:three_prime_UTR 30.0%
! GGTTGAACTTTTAGCATTTT+TGG + tig0000405:13323-13342 Msa1335440:three_prime_UTR 30.0%
!!! AGCATTTTTGGTTACAGATT+TGG + tig0000405:13335-13354 Msa1335440:three_prime_UTR 30.0%
!!! GCATTTTTGGTTACAGATTT+GGG + tig0000405:13336-13355 Msa1335440:three_prime_UTR 30.0%
TTATGTAAACCCTCACTTAA+TGG + tig0000405:13399-13418 Msa1335440:three_prime_UTR 30.0%
CTAGTAAAATGTGTTAGAAC+AGG - tig0000405:13506-13525 None:intergenic 30.0%
TGCAATCTGTCTCTTCAATT+GGG - tig0000405:10625-10644 None:intergenic 35.0%
TTGCAATCTGTCTCTTCAAT+TGG - tig0000405:10626-10645 None:intergenic 35.0%
CAAAAGGAATAGCGTGAAAT+CGG - tig0000405:10812-10831 None:intergenic 35.0%
ATTGTTAATCGAACGGCAAA+AGG - tig0000405:10828-10847 None:intergenic 35.0%
!! TAATCAACGGTTTGCTGATT+GGG - tig0000405:10885-10904 None:intergenic 35.0%
CATAGAATCAGTGATTCGTT+AGG + tig0000405:10988-11007 Msa1335440:intron 35.0%
! TAGGATCTGGCATATTGATT+TGG + tig0000405:11007-11026 Msa1335440:intron 35.0%
GAAAGACAACATTTGTGAAG+AGG + tig0000405:11072-11091 Msa1335440:CDS 35.0%
CAGTTTGTGAAGAAATCCAA+TGG - tig0000405:11268-11287 None:intergenic 35.0%
TTGGATTTCTTCACAAACTG+CGG + tig0000405:11268-11287 Msa1335440:CDS 35.0%
!!! CTGTGATCTCTAGTTTTTGA+TGG + tig0000405:11391-11410 Msa1335440:intron 35.0%
AACAGGGAATTTGGTTAAAC+AGG - tig0000405:11420-11439 None:intergenic 35.0%
GTTTAACCAAATTCCCTGTT+CGG + tig0000405:11420-11439 Msa1335440:intron 35.0%
AGTGTTACATAAACCGAACA+GGG - tig0000405:11436-11455 None:intergenic 35.0%
AAGTGTTACATAAACCGAAC+AGG - tig0000405:11437-11456 None:intergenic 35.0%
!! TCAATTGCTTGCTTTTGTAG+TGG + tig0000405:11845-11864 Msa1335440:intron 35.0%
GACATAATAGGATTCTCCTT+CGG + tig0000405:11971-11990 Msa1335440:intron 35.0%
CTTTATATTGCAGTATCCAC+GGG + tig0000405:12043-12062 Msa1335440:intron 35.0%
CATACAAAAATGTTCCTACC+TGG + tig0000405:12106-12125 Msa1335440:CDS 35.0%
TTACTAACCGACAAAGATCA+CGG - tig0000405:12134-12153 None:intergenic 35.0%
AGTGTCCATAAAGCAACATA+AGG - tig0000405:12213-12232 None:intergenic 35.0%
CTTTATGGACACTTCCTATT+TGG + tig0000405:12220-12239 Msa1335440:intron 35.0%
CCTGAAAATCAGTTCCAAAT+AGG - tig0000405:12237-12256 None:intergenic 35.0%
!! CCTATTTGGAACTGATTTTC+AGG + tig0000405:12234-12253 Msa1335440:intron 35.0%
AACATCCCAATTGTTCTTTG+CGG + tig0000405:12266-12285 Msa1335440:CDS 35.0%
TGTTACCGCAAAGAACAATT+GGG - tig0000405:12274-12293 None:intergenic 35.0%
TTGTTACCGCAAAGAACAAT+TGG - tig0000405:12275-12294 None:intergenic 35.0%
AATTGTTCTTTGCGGTAACA+AGG + tig0000405:12274-12293 Msa1335440:CDS 35.0%
! ACTGCAAATTTTTCTTCCTG+TGG - tig0000405:12343-12362 None:intergenic 35.0%
!! TTTCTAGTTTCTACACAGTC+AGG + tig0000405:12448-12467 Msa1335440:intron 35.0%
GATTCTACAAAGTGCAAGTT+AGG - tig0000405:12541-12560 None:intergenic 35.0%
GCCAAATCAATTTGAACTTC+TGG - tig0000405:12580-12599 None:intergenic 35.0%
CAACAAAGGTAAGCTATCTT+TGG + tig0000405:12604-12623 Msa1335440:intron 35.0%
! ATTGTAGCATTGGAAAGTAG+TGG + tig0000405:12684-12703 Msa1335440:intron 35.0%
!! GGTTGTGAGACTTTGATTTT+AGG - tig0000405:12729-12748 None:intergenic 35.0%
TCACAACCATCATTGATATG+AGG + tig0000405:12741-12760 Msa1335440:intron 35.0%
CACAACCATCATTGATATGA+GGG + tig0000405:12742-12761 Msa1335440:intron 35.0%
CATAACCCTCATATCAATGA+TGG - tig0000405:12750-12769 None:intergenic 35.0%
ACACAGCATTTAAACTCATG+AGG - tig0000405:12848-12867 None:intergenic 35.0%
! ACACGTGGATTAATGTTCAA+CGG - tig0000405:12972-12991 None:intergenic 35.0%
TTGATACGTCCATTAAGTGA+GGG - tig0000405:13411-13430 None:intergenic 35.0%
ATTGATACGTCCATTAAGTG+AGG - tig0000405:13412-13431 None:intergenic 35.0%
ACAAACAAAATCAATGCCTG+CGG - tig0000405:13441-13460 None:intergenic 35.0%
!!! ACTTTTTCAAGTGAAATGGG+TGG - tig0000405:13480-13499 None:intergenic 35.0%
TCTTCTTTCGAACCACACTA+TGG + tig0000405:10765-10784 Msa1335440:exon 40.0%
! TCATCGTGTTTTTCTTCTGC+AGG + tig0000405:10854-10873 Msa1335440:intron 40.0%
!! GTAATCAACGGTTTGCTGAT+TGG - tig0000405:10886-10905 None:intergenic 40.0%
ATCAGTGATTCGTTAGGATC+TGG + tig0000405:10994-11013 Msa1335440:intron 40.0%
!! TTTGTGAAGAGGCATCTTAC+TGG + tig0000405:11083-11102 Msa1335440:CDS 40.0%
GTAAACAGCTACAATTGGTG+TGG + tig0000405:11234-11253 Msa1335440:intron 40.0%
CATAAACCGAACAGGGAATT+TGG - tig0000405:11429-11448 None:intergenic 40.0%
TGCAACACACCACGTAATAT+TGG - tig0000405:11695-11714 None:intergenic 40.0%
!! TAATAAGGGTGGCTGTTTCT+TGG + tig0000405:11780-11799 Msa1335440:intron 40.0%
TTATAATAGCACATTGCCCG+TGG - tig0000405:12062-12081 None:intergenic 40.0%
!! ACAAGGTTGATGTGAAGAAC+AGG + tig0000405:12291-12310 Msa1335440:CDS 40.0%
TTCCTAGCAAGGTAAAGGAA+TGG - tig0000405:12407-12426 None:intergenic 40.0%
CTTTGTAGAATCTCCTGCAT+TGG + tig0000405:12549-12568 Msa1335440:CDS 40.0%
!! TTGATTTGGCTGCACAACAA+AGG + tig0000405:12590-12609 Msa1335440:CDS 40.0%
! ATTTCTGGGGGCATATCATT+AGG + tig0000405:12921-12940 Msa1335440:intron 40.0%
AGGGATGATGAAAAAACACG+TGG - tig0000405:12987-13006 None:intergenic 40.0%
GTACTGCATATTCCTAAACG+AGG - tig0000405:13028-13047 None:intergenic 40.0%
ATCATCATCGTCATCAGGAA+GGG - tig0000405:13219-13238 None:intergenic 40.0%
AATGCATCATCATCGTCATC+AGG - tig0000405:13224-13243 None:intergenic 40.0%
! CGATGATGATGCATTTGAGT+AGG + tig0000405:13229-13248 Msa1335440:CDS 40.0%
! TTGAAAGCGTGAAACTGACG+CGG - tig0000405:10671-10690 None:intergenic 45.0%
! ACTGAGAGCATACCATAGTG+TGG - tig0000405:10780-10799 None:intergenic 45.0%
CTTGAAGCTCGGGTAATCAA+CGG - tig0000405:10898-10917 None:intergenic 45.0%
ACGATGACAAGCTTGAAGCT+CGG - tig0000405:10909-10928 None:intergenic 45.0%
AGCTTCAAGCTTGTCATCGT+CGG + tig0000405:10909-10928 Msa1335440:CDS 45.0%
CAAGATTCGCTTCTACTGCT+GGG + tig0000405:11291-11310 Msa1335440:CDS 45.0%
ACTGCTGGACAAGAGAAGTT+TGG + tig0000405:11316-11335 Msa1335440:CDS 45.0%
! AAGTTTGGTGGTCTCAGAGA+TGG + tig0000405:11331-11350 Msa1335440:CDS 45.0%
ACAAGCACAAACAATGCCGA+AGG - tig0000405:11990-12009 None:intergenic 45.0%
TGTGAAGAACAGGCAAGTGA+AGG + tig0000405:12301-12320 Msa1335440:CDS 45.0%
! CAAAGCAGGTTACTTTCCAC+AGG + tig0000405:12324-12343 Msa1335440:CDS 45.0%
AGCCATTCCTTTACCTTGCT+AGG + tig0000405:12402-12421 Msa1335440:CDS 45.0%
CAAGCTTCCTAGCAAGGTAA+AGG - tig0000405:12412-12431 None:intergenic 45.0%
AACGAGGCGGATAAAAGGAA+GGG - tig0000405:13012-13031 None:intergenic 45.0%
AAACGAGGCGGATAAAAGGA+AGG - tig0000405:13013-13032 None:intergenic 45.0%
! TCCTTTTATCCGCCTCGTTT+AGG + tig0000405:13013-13032 Msa1335440:intron 45.0%
TCCTAAACGAGGCGGATAAA+AGG - tig0000405:13017-13036 None:intergenic 45.0%
CTGCATATTCCTAAACGAGG+CGG - tig0000405:13025-13044 None:intergenic 45.0%
TCATCATCGTCATCAGGAAG+GGG - tig0000405:13218-13237 None:intergenic 45.0%
CATCATCATCGTCATCAGGA+AGG - tig0000405:13220-13239 None:intergenic 45.0%
TGCTATCCTTTGTCTCGAGT+TGG + tig0000405:13263-13282 Msa1335440:three_prime_UTR 45.0%
CGATGACAAGCTTGAAGCTC+GGG - tig0000405:10908-10927 None:intergenic 50.0%
! TGGTGTGGAAGTGCATCCAT+TGG + tig0000405:11249-11268 Msa1335440:CDS 50.0%
GCAAGATTCGCTTCTACTGC+TGG + tig0000405:11290-11309 Msa1335440:CDS 50.0%
TTCTACTGCTGGGATACTGC+TGG + tig0000405:11301-11320 Msa1335440:CDS 50.0%
GCTGGACAAGAGAAGTTTGG+TGG + tig0000405:11319-11338 Msa1335440:CDS 50.0%
TACCTTGCTAGGAAGCTTGC+AGG + tig0000405:12413-12432 Msa1335440:CDS 50.0%
ACCTTGCTAGGAAGCTTGCA+GGG + tig0000405:12414-12433 Msa1335440:CDS 50.0%
GCGGATAAAAGGAAGGGACA+GGG - tig0000405:13006-13025 None:intergenic 50.0%
! GTCATCAGGAAGGGGTTGAT+TGG - tig0000405:13210-13229 None:intergenic 50.0%
ACAAAGGATAGCAGTCGCAC+GGG - tig0000405:13256-13275 None:intergenic 50.0%
GACAAAGGATAGCAGTCGCA+CGG - tig0000405:13257-13276 None:intergenic 50.0%
ACAGCACCAACTCGAGACAA+AGG - tig0000405:13272-13291 None:intergenic 50.0%
ACGTATCAATACTCTGCCGC+AGG + tig0000405:13422-13441 Msa1335440:three_prime_UTR 50.0%
GACGCGGCTCTTCTGTCTTT+CGG - tig0000405:10655-10674 None:intergenic 55.0%
AAGCTTGTCATCGTCGGCGA+TGG + tig0000405:10915-10934 Msa1335440:CDS 55.0%
! CAAAGATCACGGTGCCAGGT+AGG - tig0000405:12123-12142 None:intergenic 55.0%
CCTGGCACCGTGATCTTTGT+CGG + tig0000405:12124-12143 Msa1335440:CDS 55.0%
CAGGCAAGTGAAGGCAAAGC+AGG + tig0000405:12310-12329 Msa1335440:CDS 55.0%
ACCCTGCAAGCTTCCTAGCA+AGG - tig0000405:12418-12437 None:intergenic 55.0%
TGCTAGGAAGCTTGCAGGGT+AGG + tig0000405:12418-12437 Msa1335440:intron 55.0%
ACTTCTGGTGGAGCCAATGC+AGG - tig0000405:12565-12584 None:intergenic 55.0%
GGCGGATAAAAGGAAGGGAC+AGG - tig0000405:13007-13026 None:intergenic 55.0%
! GAAGGGGTTGATTGGCAGCT+TGG - tig0000405:13202-13221 None:intergenic 55.0%
CTTGTCATCGTCGGCGATGG+CGG + tig0000405:10918-10937 Msa1335440:CDS 60.0%
CCGACAAAGATCACGGTGCC+AGG - tig0000405:12127-12146 None:intergenic 60.0%
! ATCGTCGGCGATGGCGGCAC+TGG + tig0000405:10924-10943 Msa1335440:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0000405 gene 10622 13560 10622 ID=Msa1335440;Name=Msa1335440
tig0000405 mRNA 10622 13560 10622 ID=Msa1335440-mRNA-1;Parent=Msa1335440;Name=Msa1335440-mRNA-1;_AED=0.32;_eAED=0.32;_QI=162|1|1|1|0.87|0.77|9|286|221
tig0000405 exon 10622 10786 10622 ID=Msa1335440-mRNA-1:exon:163;Parent=Msa1335440-mRNA-1
tig0000405 exon 10876 10945 10876 ID=Msa1335440-mRNA-1:exon:164;Parent=Msa1335440-mRNA-1
tig0000405 exon 11072 11128 11072 ID=Msa1335440-mRNA-1:exon:165;Parent=Msa1335440-mRNA-1
tig0000405 exon 11242 11359 11242 ID=Msa1335440-mRNA-1:exon:166;Parent=Msa1335440-mRNA-1
tig0000405 exon 12056 12145 12056 ID=Msa1335440-mRNA-1:exon:167;Parent=Msa1335440-mRNA-1
tig0000405 exon 12256 12435 12256 ID=Msa1335440-mRNA-1:exon:168;Parent=Msa1335440-mRNA-1
tig0000405 exon 12534 12611 12534 ID=Msa1335440-mRNA-1:exon:169;Parent=Msa1335440-mRNA-1
tig0000405 exon 13181 13491 13181 ID=Msa1335440-mRNA-1:exon:170;Parent=Msa1335440-mRNA-1
tig0000405 exon 13516 13560 13516 ID=Msa1335440-mRNA-1:exon:171;Parent=Msa1335440-mRNA-1
tig0000405 five_prime_UTR 10622 10783 10622 ID=Msa1335440-mRNA-1:five_prime_utr;Parent=Msa1335440-mRNA-1
tig0000405 CDS 10784 10786 10784 ID=Msa1335440-mRNA-1:cds;Parent=Msa1335440-mRNA-1
tig0000405 CDS 10876 10945 10876 ID=Msa1335440-mRNA-1:cds;Parent=Msa1335440-mRNA-1
tig0000405 CDS 11072 11128 11072 ID=Msa1335440-mRNA-1:cds;Parent=Msa1335440-mRNA-1
tig0000405 CDS 11242 11359 11242 ID=Msa1335440-mRNA-1:cds;Parent=Msa1335440-mRNA-1
tig0000405 CDS 12056 12145 12056 ID=Msa1335440-mRNA-1:cds;Parent=Msa1335440-mRNA-1
tig0000405 CDS 12256 12435 12256 ID=Msa1335440-mRNA-1:cds;Parent=Msa1335440-mRNA-1
tig0000405 CDS 12534 12611 12534 ID=Msa1335440-mRNA-1:cds;Parent=Msa1335440-mRNA-1
tig0000405 CDS 13181 13250 13181 ID=Msa1335440-mRNA-1:cds;Parent=Msa1335440-mRNA-1
tig0000405 three_prime_UTR 13251 13491 13251 ID=Msa1335440-mRNA-1:three_prime_utr;Parent=Msa1335440-mRNA-1
tig0000405 three_prime_UTR 13516 13560 13516 ID=Msa1335440-mRNA-1:three_prime_utr;Parent=Msa1335440-mRNA-1
Gene Sequence

>Msa1335440

ATGGCTTTGCCCAATCAGCAAACCGTTGATTACCCGAGCTTCAAGCTTGTCATCGTCGGCGATGGCGGCACTGGAAAGACAACATTTGTGAAGAGGCATCTTACTGGAGAATTTGAGAAGAAATATGAACCTACAATTGGTGTGGAAGTGCATCCATTGGATTTCTTCACAAACTGCGGCAAGATTCGCTTCTACTGCTGGGATACTGCTGGACAAGAGAAGTTTGGTGGTCTCAGAGATGGATACTATATCCACGGGCAATGTGCTATTATAATGTTTGATGTTACTGCCCGTCTAACATACAAAAATGTTCCTACCTGGCACCGTGATCTTTGTCGGGTCTGTGAAAACATCCCAATTGTTCTTTGCGGTAACAAGGTTGATGTGAAGAACAGGCAAGTGAAGGCAAAGCAGGTTACTTTCCACAGGAAGAAAAATTTGCAGTACTATGAGATTTCAGCTAAGAGCAACTATAACTTTGAGAAGCCATTCCTTTACCTTGCTAGGAAGCTTGCAGGTGACCCTAACTTGCACTTTGTAGAATCTCCTGCATTGGCTCCACCAGAAGTTCAAATTGATTTGGCTGCACAACAAAGACATGAGAATGAGCTTCTCCAAGCTGCCAATCAACCCCTTCCTGATGACGATGATGATGCATTTGAGTAG
Protein sequence

>Msa1335440

MALPNQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQIDLAAQQRHENELLQAANQPLPDDDDDAFE*