AlfalfaGEDB | Msa0384760

M. sativa cultivar zhongmu-4 / Msa0384760

KEGG: K16670
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF07526.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0384760	XP_013462164.1	96.652	448	10	3	1	448	1	443	0.0	885

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0384760	sp\|Q1PFD1\|BLH11_ARATH	60.377	265	96	6	173	431	19	280	7.65e-105	316

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0384760	A0A072V2D2	96.652	448	10	3	1	448	1	443	0.0	885

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TFs/TRs:

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Gene ID	Type	Classification
Msa0384760	TF	HB-BELL

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0384760	Msa0479180	0.804909	1.722382e-49	-8.615850e-47
Msa0384760	Msa0479210	0.823956	1.047351e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0384760	MtrunA17_Chr3g0141931	96.652	448	10	3	1	448	1	443	0.0	885
Msa0384760	MtrunA17_Chr1g0155681	50.903	277	105	8	173	432	173	435	6.95e-78	254
Msa0384760	MtrunA17_Chr3g0141941	45.833	288	120	7	163	432	155	424	1.20e-70	232
Msa0384760	MtrunA17_Chr4g0025521	45.936	283	135	6	170	442	217	491	1.43e-66	226
Msa0384760	MtrunA17_Chr5g0403251	43.000	300	150	9	136	427	312	598	4.01e-65	223
Msa0384760	MtrunA17_Chr7g0267481	46.154	286	121	6	168	432	169	442	7.31e-65	220
Msa0384760	MtrunA17_Chr1g0177751	45.418	251	129	4	169	414	160	407	2.18e-64	219
Msa0384760	MtrunA17_Chr8g0373801	44.322	273	135	6	170	431	388	654	3.76e-63	218
Msa0384760	MtrunA17_Chr4g0008591	41.697	271	127	5	167	431	193	438	1.85e-61	210
Msa0384760	MtrunA17_Chr4g0028701	37.681	345	163	7	140	432	205	549	1.97e-61	211

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0384760	AT1G75430.1	60.377	265	96	6	173	431	19	280	7.78e-106	316
Msa0384760	AT1G75410.1	49.265	272	126	4	162	429	159	422	1.13e-77	251
Msa0384760	AT1G75410.2	49.265	272	126	4	162	429	159	422	1.13e-77	251
Msa0384760	AT1G19700.1	49.291	282	114	6	164	429	159	427	4.94e-77	250
Msa0384760	AT1G19700.3	49.291	282	114	6	164	429	159	427	4.94e-77	250
Msa0384760	AT1G19700.5	49.291	282	114	6	164	429	159	427	4.94e-77	250
Msa0384760	AT1G19700.4	49.291	282	114	6	164	429	159	427	4.94e-77	250
Msa0384760	AT1G19700.2	49.291	282	114	6	164	429	159	427	4.94e-77	250
Msa0384760	AT2G16400.1	47.059	289	129	7	148	433	98	365	8.77e-77	248
Msa0384760	AT4G34610.2	48.175	274	119	7	170	437	140	396	3.05e-70	232

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Find 79 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGTTAGCTGGCGGGT+TGG	0.557706	3_1:+88063875	None:intergenic
AAAGCAGAGTTGAGGATTCA+TGG	0.417721	3_1:-88065452	Msa0384760:CDS
AAATTGGTTCATTAATGCAA+GGG	0.576729	3_1:-88063964	Msa0384760:CDS
AACAATATTGGACTAACACA+AGG	0.620037	3_1:-88065989	Msa0384760:CDS
AACCAAGAACTACTACTAGG+TGG	0.711092	3_1:-88066076	Msa0384760:CDS
AACCCAAGTTACTTCATGCC+TGG	0.516099	3_1:-88065812	Msa0384760:CDS
AAGGGAAGATGTCACTGTGG+AGG	0.641601	3_1:-88063847	Msa0384760:CDS
AAGTTTGGAGACCTATTAGA+GGG	0.581283	3_1:-88064420	Msa0384760:CDS
AATAATCACCAGACACAACA+AGG	0.630483	3_1:-88065911	Msa0384760:CDS
AATGCATATCATGAATCACT+TGG	0.609154	3_1:-88066022	Msa0384760:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGCATATCATGAATCACT+TGG	-	chr3_1:88063819-88063838	Msa0384760:three_prime_UTR	30.0%
AACAATATTGGACTAACACA+AGG	-	chr3_1:88063852-88063871	Msa0384760:CDS	30.0%
TAAGACCTTGATTTAAGGAA+CGG	+	chr3_1:88064004-88064023	None:intergenic	30.0%
GATCATAAGACCTTGATTTA+AGG	+	chr3_1:88064009-88064028	None:intergenic	30.0%
CATAAGAAGTAGAAGTAGAA+CGG	+	chr3_1:88064193-88064212	None:intergenic	30.0%
AATAATAAGCACTTGTGAGA+CGG	+	chr3_1:88064830-88064849	None:intergenic	30.0%
GTGCTTATTATTGAGAAACA+TGG	-	chr3_1:88064838-88064857	Msa0384760:intron	30.0%
ATACTACTTCTTCCATTTGA+TGG	+	chr3_1:88065149-88065168	None:intergenic	30.0%
TGAGAAAAGAAAACTGTTTC+AGG	-	chr3_1:88065287-88065306	Msa0384760:intron	30.0%
ATTAACTTCTCAGAGTCATT+AGG	+	chr3_1:88065606-88065625	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	88063725	88066138	88063725	ID=Msa0384760;Name=Msa0384760
chr3_1	mRNA	88063725	88066138	88063725	ID=Msa0384760-mRNA-1;Parent=Msa0384760;Name=Msa0384760-mRNA-1;_AED=0.33;_eAED=0.33;_QI=1\|1\|0.75\|1\|1\|1\|4\|117\|448
chr3_1	exon	88063725	88063991	88063725	ID=Msa0384760-mRNA-1:exon:23690;Parent=Msa0384760-mRNA-1
chr3_1	exon	88064204	88064264	88064204	ID=Msa0384760-mRNA-1:exon:23689;Parent=Msa0384760-mRNA-1
chr3_1	exon	88064361	88064743	88064361	ID=Msa0384760-mRNA-1:exon:23688;Parent=Msa0384760-mRNA-1
chr3_1	exon	88065385	88066138	88065385	ID=Msa0384760-mRNA-1:exon:23687;Parent=Msa0384760-mRNA-1
chr3_1	five_prime_UTR	88066138	88066138	88066138	ID=Msa0384760-mRNA-1:five_prime_utr;Parent=Msa0384760-mRNA-1
chr3_1	CDS	88065385	88066137	88065385	ID=Msa0384760-mRNA-1:cds;Parent=Msa0384760-mRNA-1
chr3_1	CDS	88064361	88064743	88064361	ID=Msa0384760-mRNA-1:cds;Parent=Msa0384760-mRNA-1
chr3_1	CDS	88064204	88064264	88064204	ID=Msa0384760-mRNA-1:cds;Parent=Msa0384760-mRNA-1
chr3_1	CDS	88063842	88063991	88063842	ID=Msa0384760-mRNA-1:cds;Parent=Msa0384760-mRNA-1
chr3_1	three_prime_UTR	88063725	88063841	88063725	ID=Msa0384760-mRNA-1:three_prime_utr;Parent=Msa0384760-mRNA-1

Gene Sequence

>Msa0384760

ATGATGTCAAACTCCATGACCCATCAAAATCAATTTGAGAACCAAGAACTACTACTAGGTGGTGGTTATGTTTCATCTTCTTTGAGAAACAACAATGCATATCATGAATCACTTGGAACATTTTCTAACAATATTGGACTAACACAAGGTTCTGAGATGAGTCACACAAGACATTTGATGGATCTACTTGGTGCAGCAAACGAGAATAATCACCAGACACAACAAGGTCTATCCCTTTCTTTAGGCTCTCACATGCTTGTTGATGAATACAGAAACCGTTCCTTAAATCAAGGTCTTATGATCAACCCAAGTTACTTCATGCCTGGTCAAGATCAGACACGTGAGCCTTGTAATCAACCTGTGGTGGAGAATCTAACAAGTGATTATTTTTTCAATGGTGGAAATGGAGGTGGAACTTTTGCTTCAGGTTCTAATAATAATAATAATAATAACTCATTATTGAACCGTTCTACTTCTACTTCTTATGGAAGTGAGAGTTTTGGTTCTGTTATTGGGAACTCTAGATACCTTAAACCAGTACAGTCACTTCTTGAAGATCTTGTTGATGTTGGTGGAAATGTGATTGATAGAATGAATGAAAAGTATGCTGAGAAGTTGTTTCATGGGAGTAGAACAGGTGCTAGAACACTCTCCTCTGACCTCAAAGCAGAGTTGAGGATTCATGGACATTTATTGGCTGACAAACATCAACATCAAGTCAATATTGCAAAGCTCATTTCATTGTTGGATGAGGTTGAGGGTAGATATGAGAAGTACTACCATCAAATGGAAGAAGTAGTATCATCTTTTGAAATGATAGCAGGTTTGGGAGCTGCAAAGTGTTACACTGCTTTGGCACTTCAGGCAATGTCTAGGCATTTTTGTAGCTTAAGAGATGCCATAATGTCCCAAATAAATGCTGAGAAAAGAAAACTGTTTCAGGATGTACCTAAAATTAATAGTGGATTGTCTCAACTGAGCTTATTTGAGAGAGATAATAATAGACAGAATAGAATGTCTCTCCAACAACTTGGTGTCATTCAAAACCAACGACAAGTTTGGAGACCTATTAGAGGGTTACCTGAAACATCTGTAGCAATTCTTCGTGCTTGGCTCTTTGAGCACTTTCTTCATCCCTATCCTAATGACTCTGAGAAGTTAATCCTGGCTTCCCAAACAGGTTTAACCAAAAACCAAGTGTCAAATTGGTTCATTAATGCAAGGGTACGGCTATGGAAACCAATGATAGAAGAAATGTACAAAGAAGAGTTTGGAGACTCTTCCGAAGACTCCAACCCGCCAGCTAACAATTTTATGTCAAGGGAAGATGTCACTGTGGAGGATTAG

Protein sequence

>Msa0384760

MMSNSMTHQNQFENQELLLGGGYVSSSLRNNNAYHESLGTFSNNIGLTQGSEMSHTRHLMDLLGAANENNHQTQQGLSLSLGSHMLVDEYRNRSLNQGLMINPSYFMPGQDQTREPCNQPVVENLTSDYFFNGGNGGGTFASGSNNNNNNNSLLNRSTSTSYGSESFGSVIGNSRYLKPVQSLLEDLVDVGGNVIDRMNEKYAEKLFHGSRTGARTLSSDLKAELRIHGHLLADKHQHQVNIAKLISLLDEVEGRYEKYYHQMEEVVSSFEMIAGLGAAKCYTALALQAMSRHFCSLRDAIMSQINAEKRKLFQDVPKINSGLSQLSLFERDNNRQNRMSLQQLGVIQNQRQVWRPIRGLPETSVAILRAWLFEHFLHPYPNDSEKLILASQTGLTKNQVSNWFINARVRLWKPMIEEMYKEEFGDSSEDSNPPANNFMSREDVTVED*