AlfalfaGEDB | Msa0386960

M. sativa cultivar zhongmu-4 / Msa0386960

KEGG: -
GO: GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0005694 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006342 GO:0006355 GO:0006996 GO:0007275 GO:0008150 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016043 GO:0016458 GO:0017053 GO:0019219 GO:0019222 GO:0022607 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031399 GO:0031400 GO:0031497 GO:0031507 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033043 GO:0033044 GO:0035064 GO:0035065 GO:0035067 GO:0040029 GO:0042393 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044085 GO:0044212 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070828 GO:0071103 GO:0071840 GO:0080090 GO:0090568 GO:0097159 GO:0140030 GO:0140034 GO:0140110 GO:1901363 GO:1901983 GO:1901984 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000756 GO:2000757 GO:2001141 GO:2001251
PFAM: PF01426.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0386960	XP_003603985.1	61.780	191	44	3	217	378	28	218	1.56e-68	225
Msa0386960	XP_003603985.1	98.104	211	2	1	1	209	1	211	7.78e-152	437

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0386960	sp\|Q9FEN9\|SHL_ARATH	69.668	211	59	3	1	209	1	208	1.58e-107	318
Msa0386960	sp\|Q9FEN9\|SHL_ARATH	49.020	204	72	5	203	375	10	212	2.95e-55	184

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0386960	G7J7R0	61.780	191	44	3	217	378	28	218	7.46e-69	225
Msa0386960	G7J7R0	98.104	211	2	1	1	209	1	211	3.71e-152	437

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TFs/TRs:

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Gene ID	Type	Classification
Msa0386960	TR	PHD

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0386960	Msa1412220	0.870587	1.303777e-66	-8.615850e-47
Msa0386960	Msa1460200	0.848699	5.189565e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0386960	MtrunA17_Chr3g0145031	98.104	211	2	1	1	209	1	211	7.15e-156	437
Msa0386960	MtrunA17_Chr3g0145031	61.780	191	44	3	217	378	28	218	1.44e-72	225
Msa0386960	MtrunA17_Chr3g0145041	78.125	192	17	2	212	378	5	196	3.66e-100	295
Msa0386960	MtrunA17_Chr3g0145041	68.912	193	54	2	19	209	1	189	6.28e-94	279
Msa0386960	MtrunA17_Chr2g0316511	66.509	212	65	4	1	209	1	209	1.81e-95	284
Msa0386960	MtrunA17_Chr2g0316511	45.550	191	75	4	217	378	26	216	9.96e-47	159
Msa0386960	MtrunA17_Chr2g0324381	50.000	192	86	6	1	188	10	195	1.05e-57	186
Msa0386960	MtrunA17_Chr2g0324381	42.515	167	65	5	218	354	34	199	1.69e-33	123
Msa0386960	MtrunA17_Chr5g0447451	44.961	129	59	6	74	190	15	143	4.48e-27	104
Msa0386960	MtrunA17_Chr2g0324361	51.000	100	43	4	27	124	12	107	4.54e-25	98.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0386960	AT4G39100.1	69.668	211	59	3	1	209	1	208	1.61e-108	318
Msa0386960	AT4G39100.1	49.020	204	72	5	203	375	10	212	3.00e-56	184
Msa0386960	AT4G22140.2	65.877	211	66	4	2	209	10	217	1.18e-100	297
Msa0386960	AT4G22140.2	46.597	191	73	4	217	378	34	224	8.94e-52	172
Msa0386960	AT4G22140.1	65.877	211	66	4	2	209	10	217	2.04e-100	297
Msa0386960	AT4G22140.1	45.652	184	71	4	217	371	34	217	1.66e-47	161
Msa0386960	AT4G22140.3	65.877	211	66	4	2	209	10	217	2.04e-100	297
Msa0386960	AT4G22140.3	45.652	184	71	4	217	371	34	217	1.66e-47	161
Msa0386960	AT4G39100.2	75.758	165	36	2	1	163	1	163	8.10e-91	270
Msa0386960	AT4G39100.2	58.333	132	47	3	203	326	10	141	1.87e-44	151

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Find 121 sgRNAs with CRISPR-Local

Find 687 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGGTTATGGCAATT+TGG	0.283943	3_1:+90802064	None:intergenic
AAAATGCACTGTTCACAGTT+TGG	0.374580	3_1:-90794995	Msa0386960:CDS
AAACACATCCTCCACTACCT+AGG	0.542451	3_1:+90792288	None:intergenic
AAAGAAGTGGTCAAGTCTTT+CGG	0.363321	3_1:+90799108	None:intergenic
AACAAATCTGTCACAATCAA+AGG	0.523901	3_1:-90801884	Msa0386960:intron
AACAACTTTCACAAAAGAAG+TGG	0.593717	3_1:+90799095	None:intergenic
AACACAACACAACACAACCA+TGG	0.662889	3_1:-90801960	Msa0386960:exon
AACACATCCTCCACTACCTA+GGG	0.569275	3_1:+90792289	None:intergenic
AAGGACCCGATGAGGATATA+TGG	0.371664	3_1:+90792228	None:intergenic
AAGGGTCGGCGTCATTTCCA+TGG	0.570085	3_1:-90795078	Msa0386960:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAAATGCTTTGTCTTTGAG+TGG	+	chr3_1:90792204-90792223	None:intergenic	30.0%
AAAAGAAGGTTATGGCAATT+TGG	+	chr3_1:90792296-90792315	None:intergenic	30.0%
AACAAATCTGTCACAATCAA+AGG	-	chr3_1:90792473-90792492	Msa0386960:intron	30.0%
CAATCATAACATAATCCAAG+GGG	+	chr3_1:90793486-90793505	None:intergenic	30.0%
TTCAACAATGGATCTTTATC+TGG	-	chr3_1:90793594-90793613	Msa0386960:intron	30.0%
ACCAAGTACCAAAATATCAT+AGG	+	chr3_1:90793941-90793960	None:intergenic	30.0%
AATTCTTAATAGCAGCTCAT+AGG	-	chr3_1:90793983-90794002	Msa0386960:intron	30.0%
TAGATATGTGTATGCTTGTT+TGG	-	chr3_1:90794175-90794194	Msa0386960:intron	30.0%
CATGGCATATGATATGATAT+AGG	-	chr3_1:90794262-90794281	Msa0386960:intron	30.0%
TCCACTTGGATAGATATTTA+CGG	+	chr3_1:90794653-90794672	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	90792125	90802254	90792125	ID=Msa0386960;Name=Msa0386960
chr3_1	mRNA	90792125	90802254	90792125	ID=Msa0386960-mRNA-1;Parent=Msa0386960;Name=Msa0386960-mRNA-1;_AED=0.19;_eAED=0.20;_QI=291\|0.44\|0.4\|1\|0.44\|0.5\|10\|312\|378
chr3_1	exon	90792125	90792463	90792125	ID=Msa0386960-mRNA-1:exon:25215;Parent=Msa0386960-mRNA-1
chr3_1	exon	90793285	90793420	90793285	ID=Msa0386960-mRNA-1:exon:25214;Parent=Msa0386960-mRNA-1
chr3_1	exon	90794895	90795037	90794895	ID=Msa0386960-mRNA-1:exon:25213;Parent=Msa0386960-mRNA-1
chr3_1	exon	90795044	90795228	90795044	ID=Msa0386960-mRNA-1:exon:25212;Parent=Msa0386960-mRNA-1
chr3_1	exon	90798031	90798052	90798031	ID=Msa0386960-mRNA-1:exon:25211;Parent=Msa0386960-mRNA-1
chr3_1	exon	90799033	90799168	90799033	ID=Msa0386960-mRNA-1:exon:25210;Parent=Msa0386960-mRNA-1
chr3_1	exon	90800650	90800718	90800650	ID=Msa0386960-mRNA-1:exon:25209;Parent=Msa0386960-mRNA-1
chr3_1	exon	90801459	90801601	90801459	ID=Msa0386960-mRNA-1:exon:25208;Parent=Msa0386960-mRNA-1
chr3_1	exon	90801608	90801804	90801608	ID=Msa0386960-mRNA-1:exon:25207;Parent=Msa0386960-mRNA-1
chr3_1	exon	90801885	90802254	90801885	ID=Msa0386960-mRNA-1:exon:25206;Parent=Msa0386960-mRNA-1
chr3_1	five_prime_UTR	90801964	90802254	90801964	ID=Msa0386960-mRNA-1:five_prime_utr;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90801885	90801963	90801885	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90801608	90801804	90801608	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90801459	90801601	90801459	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90800650	90800718	90800650	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90799033	90799168	90799033	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90798031	90798052	90798031	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90795044	90795228	90795044	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90794895	90795037	90794895	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90793285	90793420	90793285	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	CDS	90792437	90792463	90792437	ID=Msa0386960-mRNA-1:cds;Parent=Msa0386960-mRNA-1
chr3_1	three_prime_UTR	90792125	90792436	90792125	ID=Msa0386960-mRNA-1:three_prime_utr;Parent=Msa0386960-mRNA-1

Gene Sequence

>Msa0386960

ATGGCTAAACCAAAAGCCCCAAAGCGAAACCTAGAGTCATACTCAGTCAAACATATGAACAAATCTGTCACAATCAAAGCTGGGGATTGTGTGCTTATGCGTCCATCTGATCCATCCAAACCCTCTTACGTGGCAAAGATCGAAGGGATCGAAGCTGATTCACGAGGTGCTAACGTTAAAGTTAACGTCCGGTGGTATTACCGGCCGGAGGAGTCAATTGGTGGCCGGAGACAGTTTCACGGTTCAAAGGAGCTTTTTCTGTCAGATCATTTTGATAGTGCTGATACAATTGAAGGAAAGTGTATTGTTCATGGTTTCAAGAGCTACACTAAGCTTGATGCTGTTGGTAATGATGATTTCTTCTGTCGATTTGAGTACAATTCTGCTACTGGTGCTTTCAATCCTGATAGAGTTGCAGTGTATTGTAAATGTGAGATGCCCTACAACCCTGATGATCTGATGGTTCAGTGTGAGGGCTGCAGTGACTGGTTTCATCCTGCTTGTATAGACATGACTGTGGAGGAAGCCGAAAGACTTGACCACTTCTTTTGTGAAAGTTGTTCCGTTGAAGGTCAAAAACAGCTGCAAAATTCTCATTCTGCTACAAGACTCGCAGATACAAAGATTCTCTATCTATCCCCATCGGAGGGTGATTGTGTACGCATGCGTTCCTCTAAACCGTGGATACCAACATATGTGGCTATGATCGAAGAGATGAAAGCTGATTCAAGAGACGTTCGTGTCCGTTGGTACTACTGGCCGGAGGAGACAAAGAAGGGTCGGCGTCATTTCCATGGCTCAAAGGAGCTTTTTCTATCTGATCATTTAGATAGTGTTGATACAATCGAAGGAAAATGCACTGTTCACAGTTTGGAGAACTACATGAAGCTTGATGTTGTTGGTGATGATGATTTCTTCTGTCGATTGAACTACAATTCCGCTACTGGTGCTCTCACTCCTGATATAGTTCAAGTGTTTCATCCTGCTTGTATAGACATGACGGTGGAAGAAGCCGAAAGAATTGACAACTTCATTTGTGAAAGTTGTTCTGCCGAAGTTCAAAAAAAGTTGCAAGATTCTCATTCTGCTGCAAGACTCCCAAATACAAAGGTGGATACTAAACGCCGTCAAAGATGA

Protein sequence

>Msa0386960

MAKPKAPKRNLESYSVKHMNKSVTIKAGDCVLMRPSDPSKPSYVAKIEGIEADSRGANVKVNVRWYYRPEESIGGRRQFHGSKELFLSDHFDSADTIEGKCIVHGFKSYTKLDAVGNDDFFCRFEYNSATGAFNPDRVAVYCKCEMPYNPDDLMVQCEGCSDWFHPACIDMTVEEAERLDHFFCESCSVEGQKQLQNSHSATRLADTKILYLSPSEGDCVRMRSSKPWIPTYVAMIEEMKADSRDVRVRWYYWPEETKKGRRHFHGSKELFLSDHLDSVDTIEGKCTVHSLENYMKLDVVGDDDFFCRLNYNSATGALTPDIVQVFHPACIDMTVEEAERIDNFICESCSAEVQKKLQDSHSAARLPNTKVDTKRRQR*