Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0167500 | Msa0389920 | -0.801389 | 9.215421e-49 | -8.615850e-47 |
| Msa0389920 | Msa0530560 | 0.812999 | 3.195019e-51 | -8.615850e-47 |
| Msa0389920 | Msa1171040 | 0.903889 | 2.173139e-79 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGTCGAACATGGTGCTTT+TGG | 0.302406 | 3_2:-476443 | Msa0389920:CDS |
| TCATCATGTAAGCTCTCTTT+TGG | 0.315060 | 3_2:+476498 | None:intergenic |
| AATGGACATTGAAAGGCATT+TGG | 0.392564 | 3_2:-476328 | Msa0389920:intron |
| GCCTCCCCTTCTCCTTCTCT+TGG | 0.408598 | 3_2:+476285 | None:intergenic |
| ACCATGTTCGACCTCATTCA+TGG | 0.451070 | 3_2:+476451 | None:intergenic |
| CCTTCTCCTTCTCTTGGCTA+TGG | 0.482678 | 3_2:+476291 | None:intergenic |
| CATTGAAAGGCATTTGGTGG+TGG | 0.489006 | 3_2:-476322 | Msa0389920:intron |
| TGAAAGGCATTTGGTGGTGG+AGG | 0.502665 | 3_2:-476319 | Msa0389920:intron |
| CATAGCCAAGAGAAGGAGAA+GGG | 0.530429 | 3_2:-476290 | Msa0389920:CDS |
| AAAGAGAGCTTACATGATGA+AGG | 0.536545 | 3_2:-476495 | Msa0389920:CDS |
| GGCATTTGGTGGTGGAGGCG+CGG | 0.542675 | 3_2:-476314 | Msa0389920:CDS |
| GAAGGAGAAGGGGAGGCGCT+AGG | 0.554494 | 3_2:-476279 | None:intergenic |
| GCGCGGCCATAGCCAAGAGA+AGG | 0.563830 | 3_2:-476297 | Msa0389920:CDS |
| TGCCCAAATCATGATGAAAG+AGG | 0.568034 | 3_2:-476522 | None:intergenic |
| GCCAAGAGAAGGAGAAGGGG+AGG | 0.584905 | 3_2:-476286 | Msa0389920:CDS |
| CCATAGCCAAGAGAAGGAGA+AGG | 0.590233 | 3_2:-476291 | Msa0389920:CDS |
| GGACATTGAAAGGCATTTGG+TGG | 0.610230 | 3_2:-476325 | Msa0389920:intron |
| GCTGCAAAACTCCATGAATG+AGG | 0.615989 | 3_2:-476462 | Msa0389920:CDS |
| TCCATGAATGAGGTCGAACA+TGG | 0.622123 | 3_2:-476452 | Msa0389920:CDS |
| ACATGATGAAGGTTCCAGAT+CGG | 0.634970 | 3_2:-476484 | Msa0389920:CDS |
| ATAGCCAAGAGAAGGAGAAG+GGG | 0.639191 | 3_2:-476289 | Msa0389920:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGATGAAATCATAAAGT+TGG | - | chr3_2:476429-476448 | Msa0389920:intron | 20.0% |
| !!! | TAAAGTTGGTAGTGTTTTAA+TGG | - | chr3_2:476443-476462 | Msa0389920:CDS | 25.0% |
| !!! | GTGTTTTAATGGACATTGAA+AGG | - | chr3_2:476454-476473 | Msa0389920:CDS | 30.0% |
| ! | TCATCATGTAAGCTCTCTTT+TGG | + | chr3_2:476294-476313 | None:intergenic | 35.0% |
| AAAGAGAGCTTACATGATGA+AGG | - | chr3_2:476294-476313 | Msa0389920:CDS | 35.0% | |
| !! | AATGGACATTGAAAGGCATT+TGG | - | chr3_2:476461-476480 | Msa0389920:CDS | 35.0% |
| ACATGATGAAGGTTCCAGAT+CGG | - | chr3_2:476305-476324 | Msa0389920:CDS | 40.0% | |
| GCTGCAAAACTCCATGAATG+AGG | - | chr3_2:476327-476346 | Msa0389920:intron | 45.0% | |
| TCCATGAATGAGGTCGAACA+TGG | - | chr3_2:476337-476356 | Msa0389920:intron | 45.0% | |
| ACCATGTTCGACCTCATTCA+TGG | + | chr3_2:476341-476360 | None:intergenic | 45.0% | |
| !! | GGACATTGAAAGGCATTTGG+TGG | - | chr3_2:476464-476483 | Msa0389920:CDS | 45.0% |
| !! | CATTGAAAGGCATTTGGTGG+TGG | - | chr3_2:476467-476486 | Msa0389920:CDS | 45.0% |
| CATAGCCAAGAGAAGGAGAA+GGG | - | chr3_2:476499-476518 | Msa0389920:CDS | 45.0% | |
| ATAGCCAAGAGAAGGAGAAG+GGG | - | chr3_2:476500-476519 | Msa0389920:CDS | 45.0% | |
| ! | ATGGAGTTTTGCAGCCGATC+TGG | + | chr3_2:476322-476341 | None:intergenic | 50.0% |
| ! | GAGGTCGAACATGGTGCTTT+TGG | - | chr3_2:476346-476365 | Msa0389920:intron | 50.0% |
| ! | GTCGAACATGGTGCTTTTGG+AGG | - | chr3_2:476349-476368 | Msa0389920:intron | 50.0% |
| !! | GAACATGGTGCTTTTGGAGG+AGG | - | chr3_2:476352-476371 | Msa0389920:intron | 50.0% |
| !! | TGAAAGGCATTTGGTGGTGG+AGG | - | chr3_2:476470-476489 | Msa0389920:CDS | 50.0% |
| CCTTCTCCTTCTCTTGGCTA+TGG | + | chr3_2:476501-476520 | None:intergenic | 50.0% | |
| CCATAGCCAAGAGAAGGAGA+AGG | - | chr3_2:476498-476517 | Msa0389920:CDS | 50.0% | |
| GCCAAGAGAAGGAGAAGGGG+AGG | - | chr3_2:476503-476522 | Msa0389920:CDS | 60.0% | |
| GCCTCCCCTTCTCCTTCTCT+TGG | + | chr3_2:476507-476526 | None:intergenic | 60.0% | |
| !! | GGCATTTGGTGGTGGAGGCG+CGG | - | chr3_2:476475-476494 | Msa0389920:CDS | 65.0% |
| GCGCGGCCATAGCCAAGAGA+AGG | - | chr3_2:476492-476511 | Msa0389920:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 476280 | 476531 | 476280 | ID=Msa0389920;Name=Msa0389920 |
| chr3_2 | mRNA | 476280 | 476531 | 476280 | ID=Msa0389920-mRNA-1;Parent=Msa0389920;Name=Msa0389920-mRNA-1;_AED=0.17;_eAED=0.55;_QI=0|0|0|1|1|1|2|0|49 |
| chr3_2 | exon | 476438 | 476531 | 476438 | ID=Msa0389920-mRNA-1:exon:107;Parent=Msa0389920-mRNA-1 |
| chr3_2 | exon | 476280 | 476335 | 476280 | ID=Msa0389920-mRNA-1:exon:106;Parent=Msa0389920-mRNA-1 |
| chr3_2 | CDS | 476438 | 476531 | 476438 | ID=Msa0389920-mRNA-1:cds;Parent=Msa0389920-mRNA-1 |
| chr3_2 | CDS | 476280 | 476335 | 476280 | ID=Msa0389920-mRNA-1:cds;Parent=Msa0389920-mRNA-1 |
| Gene Sequence |
| Protein sequence |
