Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393770 | XP_003598217.2 | 95.146 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 2.30e-64 | 200 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393770 | sp|Q9S7A3|MOC2A_ARATH | 65.263 | 95 | 30 | 1 | 12 | 103 | 2 | 96 | 1.02e-36 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393770 | G7IYH1 | 95.146 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.10e-64 | 200 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0234430 | Msa0393770 | 0.809605 | 1.742626e-50 | -8.615850e-47 |
| Msa0393770 | Msa0736190 | 0.803345 | 3.643734e-49 | -8.615850e-47 |
| Msa0393770 | Msa0906990 | 0.812317 | 4.505380e-51 | -8.615850e-47 |
| Msa0393770 | Msa0997310 | 0.800006 | 1.764704e-48 | -8.615850e-47 |
| Msa0393770 | Msa1014540 | 0.800608 | 1.330635e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393770 | MtrunA17_Chr3g0079311 | 95.146 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 2.11e-68 | 200 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393770 | AT4G10100.3 | 65.263 | 95 | 30 | 1 | 12 | 103 | 2 | 96 | 1.04e-37 | 122 |
| Msa0393770 | AT4G10100.1 | 65.263 | 95 | 30 | 1 | 12 | 103 | 2 | 96 | 1.04e-37 | 122 |
| Msa0393770 | AT4G10100.2 | 65.263 | 95 | 30 | 1 | 12 | 103 | 2 | 96 | 1.04e-37 | 122 |
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAGTTTGGAAGAAATAAA+AGG | 0.205219 | 3_2:+7913335 | Msa0393770:CDS |
| TTTAATTAACCACCACTTAT+TGG | 0.220244 | 3_2:-7913440 | None:intergenic |
| AATAAAAGGGTGCATGGTTT+TGG | 0.285137 | 3_2:+7913349 | Msa0393770:CDS |
| TCTTGCTCAATTTCCAAGTT+TGG | 0.287548 | 3_2:+7913322 | Msa0393770:CDS |
| GCACGAGCGCGGGATCTTAC+CGG | 0.315061 | 3_2:+7913236 | Msa0393770:CDS |
| GGAAGGAAATGTTAGTGTTT+GGG | 0.409388 | 3_2:+7913151 | Msa0393770:CDS |
| GTGCCATTGGAGGTGTCATC+TGG | 0.429078 | 3_2:+7913269 | Msa0393770:CDS |
| TGGAAGGAAATGTTAGTGTT+TGG | 0.429275 | 3_2:+7913150 | Msa0393770:CDS |
| CTCATCCTTGTCTTTAACAA+TGG | 0.440598 | 3_2:-7913402 | None:intergenic |
| CGGCTTGAGTGAGGTGCCAT+TGG | 0.445031 | 3_2:+7913256 | Msa0393770:CDS |
| CAAGTTTGGAAGAAATAAAA+GGG | 0.452506 | 3_2:+7913336 | Msa0393770:CDS |
| ACCACTTATTGGAGGTATGA+TGG | 0.495954 | 3_2:-7913429 | None:intergenic |
| GGAAGAAATAAAAGGGTGCA+TGG | 0.517635 | 3_2:+7913343 | Msa0393770:CDS |
| CTTGAGTGAGGTGCCATTGG+AGG | 0.521790 | 3_2:+7913259 | Msa0393770:CDS |
| TGTTAAAGACAAGGATGAGT+TGG | 0.525125 | 3_2:+7913406 | Msa0393770:CDS |
| CAATGGCACCTCACTCAAGC+CGG | 0.552411 | 3_2:-7913255 | None:intergenic |
| AATTAACCACCACTTATTGG+AGG | 0.552937 | 3_2:-7913437 | None:intergenic |
| GGAAATGTTAGTGTTTGGGA+TGG | 0.578585 | 3_2:+7913155 | Msa0393770:CDS |
| GCCATCATACCTCCAATAAG+TGG | 0.578606 | 3_2:+7913428 | Msa0393770:CDS |
| TTTACGACATATATGATGGA+AGG | 0.601290 | 3_2:+7913134 | None:intergenic |
| GGATCTTACCGGCTTGAGTG+AGG | 0.604277 | 3_2:+7913247 | Msa0393770:CDS |
| CTTCCAGATGACACCTCCAA+TGG | 0.605197 | 3_2:-7913272 | None:intergenic |
| TTCAACCATTGTTAAAGACA+AGG | 0.623881 | 3_2:+7913397 | Msa0393770:CDS |
| ATCATACCTCCAATAAGTGG+TGG | 0.650420 | 3_2:+7913431 | Msa0393770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCTTTTTTGGTTTGAGTT+TGG | - | chr3_2:7913184-7913203 | None:intergenic | 25.0% |
| ! | AACCAAAAAAGAAAGTTCTT+TGG | + | chr3_2:7913190-7913209 | Msa0393770:CDS | 25.0% |
| ! | CAAGTTTGGAAGAAATAAAA+GGG | + | chr3_2:7913336-7913355 | Msa0393770:CDS | 25.0% |
| !!! | CACCAAAGAACTTTCTTTTT+TGG | - | chr3_2:7913195-7913214 | None:intergenic | 30.0% |
| !! | CCTTTTATTTCTTCCAAACT+TGG | - | chr3_2:7913338-7913357 | None:intergenic | 30.0% |
| CCAAGTTTGGAAGAAATAAA+AGG | + | chr3_2:7913335-7913354 | Msa0393770:CDS | 30.0% | |
| TTCAACCATTGTTAAAGACA+AGG | + | chr3_2:7913397-7913416 | Msa0393770:CDS | 30.0% | |
| TGGAAGGAAATGTTAGTGTT+TGG | + | chr3_2:7913150-7913169 | Msa0393770:CDS | 35.0% | |
| GGAAGGAAATGTTAGTGTTT+GGG | + | chr3_2:7913151-7913170 | Msa0393770:CDS | 35.0% | |
| TCTTGCTCAATTTCCAAGTT+TGG | + | chr3_2:7913322-7913341 | Msa0393770:CDS | 35.0% | |
| !! | AATAAAAGGGTGCATGGTTT+TGG | + | chr3_2:7913349-7913368 | Msa0393770:CDS | 35.0% |
| CTCATCCTTGTCTTTAACAA+TGG | - | chr3_2:7913405-7913424 | None:intergenic | 35.0% | |
| TGTTAAAGACAAGGATGAGT+TGG | + | chr3_2:7913406-7913425 | Msa0393770:CDS | 35.0% | |
| GGAAATGTTAGTGTTTGGGA+TGG | + | chr3_2:7913155-7913174 | Msa0393770:CDS | 40.0% | |
| ! | GGAAGAAATAAAAGGGTGCA+TGG | + | chr3_2:7913343-7913362 | Msa0393770:CDS | 40.0% |
| !!! | CATGGTTTTGGCTTTGAATG+AGG | + | chr3_2:7913361-7913380 | Msa0393770:CDS | 40.0% |
| ! | ACCACTTATTGGAGGTATGA+TGG | - | chr3_2:7913432-7913451 | None:intergenic | 40.0% |
| ATCATACCTCCAATAAGTGG+TGG | + | chr3_2:7913431-7913450 | Msa0393770:CDS | 40.0% | |
| GCCATCATACCTCCAATAAG+TGG | + | chr3_2:7913428-7913447 | Msa0393770:CDS | 45.0% | |
| !! | TACTGTTTTTCGCACGAGCG+CGG | + | chr3_2:7913225-7913244 | Msa0393770:CDS | 50.0% |
| CTTCCAGATGACACCTCCAA+TGG | - | chr3_2:7913275-7913294 | None:intergenic | 50.0% | |
| !! | ACTGTTTTTCGCACGAGCGC+GGG | + | chr3_2:7913226-7913245 | Msa0393770:CDS | 55.0% |
| !! | GGATCTTACCGGCTTGAGTG+AGG | + | chr3_2:7913247-7913266 | Msa0393770:CDS | 55.0% |
| CAATGGCACCTCACTCAAGC+CGG | - | chr3_2:7913258-7913277 | None:intergenic | 55.0% | |
| !! | CTTGAGTGAGGTGCCATTGG+AGG | + | chr3_2:7913259-7913278 | Msa0393770:CDS | 55.0% |
| !! | GTGCCATTGGAGGTGTCATC+TGG | + | chr3_2:7913269-7913288 | Msa0393770:CDS | 55.0% |
| ! | CGGCTTGAGTGAGGTGCCAT+TGG | + | chr3_2:7913256-7913275 | Msa0393770:CDS | 60.0% |
| GCACGAGCGCGGGATCTTAC+CGG | + | chr3_2:7913236-7913255 | Msa0393770:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 7913146 | 7913457 | 7913146 | ID=Msa0393770;Name=Msa0393770 |
| chr3_2 | mRNA | 7913146 | 7913457 | 7913146 | ID=Msa0393770-mRNA-1;Parent=Msa0393770;Name=Msa0393770-mRNA-1;_AED=0.32;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|103 |
| chr3_2 | exon | 7913146 | 7913457 | 7913146 | ID=Msa0393770-mRNA-1:exon:2082;Parent=Msa0393770-mRNA-1 |
| chr3_2 | CDS | 7913146 | 7913457 | 7913146 | ID=Msa0393770-mRNA-1:cds;Parent=Msa0393770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
