AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0736190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 97 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGAAATATGAAGTTTAAA+TGG 0.149590 5_1:-52259829 None:intergenic
CTCCAGCTGGAGTCAGATTT+TGG 0.184824 5_1:+52256316 Msa0736190:CDS
GTCCTAATTTGCAGAATAAA+TGG 0.187586 5_1:+52259090 Msa0736190:intron
ACTTATATATGTTATGAAAT+TGG 0.220164 5_1:-52255451 None:intergenic
TATGAGAAATTTGTGTATTT+AGG 0.278442 5_1:+52256350 Msa0736190:CDS
ACCCGTACAGTCTTCTTCTT+AGG 0.292295 5_1:-52259149 None:intergenic
AATTGGATAACAAAGTTAAA+TGG 0.292488 5_1:+52256237 Msa0736190:CDS
AATTCTTGGTTTAGGTGTAT+TGG 0.299299 5_1:+52259521 Msa0736190:three_prime_UTR
TTCAGCATCAATTCCAAATC+TGG 0.309760 5_1:-52258902 None:intergenic
TTTGGGCAGGCATTGGGCAT+TGG 0.319761 5_1:+52258423 Msa0736190:intron
TCTACAGTGGACTCCAGATT+TGG 0.325328 5_1:+52258889 Msa0736190:intron
TGTAAGCTGGCTAGGTCTAA+TGG 0.330328 5_1:+52258209 Msa0736190:CDS
TTGGGCAGGCATTGGGCATT+GGG 0.331705 5_1:+52258424 Msa0736190:intron
GTTCGAAAATTGCTGTTAAG+AGG 0.349529 5_1:-52259364 None:intergenic
TTTCCATCGACAGACTTCTT+TGG 0.350193 5_1:+52259268 Msa0736190:intron
TATAGTAGTGCATGTGGATT+AGG 0.356282 5_1:+52259666 Msa0736190:three_prime_UTR
TATAGTAGTGCATGTGGATT+AGG 0.356282 5_1:+52259728 Msa0736190:three_prime_UTR
GAAGACGCTGTGGCAGACTA+TGG 0.357365 5_1:+52256284 Msa0736190:CDS
TTCTGCTGTGCCTGCTGTGA+TGG 0.363537 5_1:+52259322 Msa0736190:CDS
TGTGTCTTTGGGCAGGCATT+GGG 0.368874 5_1:+52258417 Msa0736190:intron
CCAGGCTCAGCAGAAGAATT+AGG 0.378579 5_1:-52259296 None:intergenic
TAAATCAGAATCCATCCTTA+AGG 0.383863 5_1:+52258964 Msa0736190:CDS
GTAAGCTGGCTAGGTCTAAT+GGG 0.391663 5_1:+52258210 Msa0736190:CDS
AATTTGTGTATTTAGGCATT+GGG 0.396459 5_1:+52256357 Msa0736190:CDS
GAAGTCTGTGGCATTGGAAT+TGG 0.398089 5_1:+52256220 Msa0736190:CDS
TCCTAATTTGCAGAATAAAT+GGG 0.404078 5_1:+52259091 Msa0736190:intron
AGGAAATATGAAGTTTAAAT+GGG 0.410225 5_1:-52259828 None:intergenic
CAAAACTCAACCACTCCTTC+CGG 0.418932 5_1:-52259468 None:intergenic
AAATTTGTGTATTTAGGCAT+TGG 0.429814 5_1:+52256356 Msa0736190:CDS
GGGAAGCCCGTCAGCTAGCT+AGG 0.436166 5_1:+52258288 Msa0736190:CDS
TTGCTCGTGAATGTGATTCA+TGG 0.439097 5_1:+52259551 Msa0736190:three_prime_UTR
CAAGATGAAGTCTGTGGCAT+TGG 0.445821 5_1:+52256214 Msa0736190:CDS
CTTGTCTGCTGCAGCTTAAG+TGG 0.445957 5_1:+52258180 Msa0736190:intron
AGAGGCAGCAACAAATTCTC+AGG 0.446494 5_1:+52255562 Msa0736190:five_prime_UTR
GGCTAGGTCTAATGGGTTTG+AGG 0.450577 5_1:+52258217 Msa0736190:CDS
GAGAGATCAGAGACGGTTAG+AGG 0.451933 5_1:+52255544 Msa0736190:five_prime_UTR
CCTAATTCTTCTGCTGAGCC+TGG 0.453232 5_1:+52259296 Msa0736190:CDS
CTAGGTCTAATGCAGGGACT+CGG 0.461461 5_1:+52258306 Msa0736190:CDS
ACAAACAAAGTTCCTCTACT+TGG 0.463453 5_1:-52255520 None:intergenic
AGCCAAAATCTGACTCCAGC+TGG 0.478708 5_1:-52256318 None:intergenic
TGGTGTATATACTATATAGT+GGG 0.494321 5_1:+52259571 Msa0736190:three_prime_UTR
ATGGTGTATATACTATATAG+TGG 0.495581 5_1:+52259570 Msa0736190:three_prime_UTR
GAGCAAAATCAACATCCTTA+AGG 0.503039 5_1:-52258979 None:intergenic
GGGAAGCTTGTAAGCTGGCT+AGG 0.506798 5_1:+52258201 Msa0736190:CDS
GCAGGCACAGCAGAAGTACC+AGG 0.511520 5_1:-52259314 None:intergenic
TAGGTCTAATGCAGGGACTC+GGG 0.513819 5_1:+52258307 Msa0736190:CDS
TAACATATATAAGTTCCTCT+TGG 0.523535 5_1:+52255460 Msa0736190:five_prime_UTR
TACTGCGAATGCAGGTATCA+AGG 0.530740 5_1:+52259394 Msa0736190:CDS
CATTGGGGAACGTGAACTAA+AGG 0.533880 5_1:+52256373 Msa0736190:CDS
CAGATGGTTACTGCGAATGC+AGG 0.537426 5_1:+52259386 Msa0736190:CDS
ATCCTAAGAAGAAGACTGTA+CGG 0.543822 5_1:+52259147 Msa0736190:CDS
GAGTAGCTAGCCGGAAGGAG+TGG 0.546791 5_1:+52259458 Msa0736190:three_prime_UTR
CACAGAACAATAACACTCAC+TGG 0.547496 5_1:-52259638 None:intergenic
TTGTCTGCTGCAGCTTAAGT+GGG 0.549453 5_1:+52258181 Msa0736190:intron
ATGGATCTGATGACTCCAGC+TGG 0.553415 5_1:+52256303 Msa0736190:CDS
CAGCTAGCTAGGTCTAATGC+AGG 0.557805 5_1:+52258299 Msa0736190:CDS
GATGGGAAAGAAGTATAGAG+AGG 0.558505 5_1:-52255342 None:intergenic
AAAAGCACAAAAGCTGAAGA+GGG 0.560388 5_1:+52258268 Msa0736190:CDS
GTTAAGAGGTTCACAACAGC+AGG 0.566554 5_1:-52259350 None:intergenic
CCTAATTTGCAGAATAAATG+GGG 0.566683 5_1:+52259092 Msa0736190:intron
TTTGTACATAGCAGCAGTGG+TGG 0.567461 5_1:-52259776 None:intergenic
CATCATGAGTCCATTTGTGA+AGG 0.572977 5_1:-52259003 None:intergenic
AAAATCAACATCCTTAAGGA+TGG 0.578151 5_1:-52258975 None:intergenic
TGAAGGATAGAAAGTGAAGG+TGG 0.579969 5_1:+52259432 Msa0736190:exon
GCAGGCACATCCATCACAGC+AGG 0.580554 5_1:-52259332 None:intergenic
CAGTGAGTGTTATTGTTCTG+TGG 0.584724 5_1:+52259639 Msa0736190:three_prime_UTR
AAAGATGCAGTAGATATACA+TGG 0.585581 5_1:+52258923 Msa0736190:CDS
GAATGAGTGTTATTGTTCTG+TGG 0.587586 5_1:+52259701 Msa0736190:three_prime_UTR
AAGATGCTTGTTATCAGAGA+GGG 0.590205 5_1:+52255422 Msa0736190:five_prime_UTR
TGCTCTGCACTTCCAAGTAG+AGG 0.593164 5_1:+52255508 Msa0736190:five_prime_UTR
CATTGGGAAACGTGAACCAA+AGG 0.594101 5_1:+52258440 Msa0736190:CDS
ATCATGAGTCCATTTGTGAA+GGG 0.594860 5_1:-52259002 None:intergenic
TCCTAAGAAGAAGACTGTAC+GGG 0.599111 5_1:+52259148 Msa0736190:CDS
TAAGTGGGAAGCTTGTAAGC+TGG 0.605300 5_1:+52258196 Msa0736190:CDS
AGGTGGAAAGAGTAGCTAGC+CGG 0.605811 5_1:+52259449 Msa0736190:three_prime_UTR
ATGGACTCATGATGCACTGA+GGG 0.605994 5_1:+52259012 Msa0736190:CDS
GTTTGTCGAGAGATCAGAGA+CGG 0.606401 5_1:+52255537 Msa0736190:five_prime_UTR
ATTTGTGTATTTAGGCATTG+GGG 0.609636 5_1:+52256358 Msa0736190:CDS
TGCAGTAGATATACATGGGG+AGG 0.612105 5_1:+52258928 Msa0736190:CDS
GGTTAATGAAAACATCAGAA+AGG 0.612698 5_1:+52259169 Msa0736190:CDS
AGGTCTAATGCAGGGACTCG+GGG 0.616145 5_1:+52258308 Msa0736190:CDS
GTCTCTGCTTGAAGACGCTG+TGG 0.621674 5_1:+52256274 Msa0736190:CDS
TCATGAGTCCATTTGTGAAG+GGG 0.624080 5_1:-52259001 None:intergenic
GGTGTCTATAGTAGTGCATG+TGG 0.631425 5_1:+52259660 Msa0736190:three_prime_UTR
GGTGTCTATAGTAGTGCATG+TGG 0.631425 5_1:+52259722 Msa0736190:three_prime_UTR
CATTAGACCTAGCTAGCTGA+CGG 0.635381 5_1:-52258295 None:intergenic
AAGATGCAGTAGATATACAT+GGG 0.637221 5_1:+52258924 Msa0736190:CDS
ATTAGACCTAGCTAGCTGAC+GGG 0.642091 5_1:-52258294 None:intergenic
GGTGTATATACTATATAGTG+GGG 0.649578 5_1:+52259572 Msa0736190:three_prime_UTR
AGCTAGCTAGGTCTAATGCA+GGG 0.656329 5_1:+52258300 Msa0736190:CDS
CAAGATGCTTGTTATCAGAG+AGG 0.661100 5_1:+52255421 Msa0736190:five_prime_UTR
ACTCTAGAAAGTTCGCCAAG+AGG 0.661447 5_1:-52255475 None:intergenic
GGAAAGAGTAGCTAGCCGGA+AGG 0.663905 5_1:+52259453 Msa0736190:three_prime_UTR
CTAATTTGCAGAATAAATGG+GGG 0.682270 5_1:+52259093 Msa0736190:intron
AGATCGCAAGATGAAGTCTG+TGG 0.683681 5_1:+52256208 Msa0736190:CDS
AGATGCAGTAGATATACATG+GGG 0.716090 5_1:+52258925 Msa0736190:CDS
AATGGACTCATGATGCACTG+AGG 0.724301 5_1:+52259011 Msa0736190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATAGAAAAAATAATAAA+AGG - chr5_1:52255296-52255315 None:intergenic 10.0%
!!! TTATTTTATTTTTTACCTTT+GGG - chr5_1:52258352-52258371 None:intergenic 10.0%
!!! TTTATTTTATTTTTTACCTT+TGG - chr5_1:52258353-52258372 None:intergenic 10.0%
!! ACTTATATATGTTATGAAAT+TGG - chr5_1:52255454-52255473 None:intergenic 15.0%
!!! TTTACTTTTTATACATGAAT+TGG + chr5_1:52256439-52256458 Msa0736190:intron 15.0%
!! AATTGTTATAGATTAATTAG+TGG + chr5_1:52257619-52257638 Msa0736190:intron 15.0%
!!! TAAGAAAAGTGTATAAATAA+AGG + chr5_1:52257758-52257777 Msa0736190:intron 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG + chr5_1:52257759-52257778 Msa0736190:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + chr5_1:52257823-52257842 Msa0736190:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG + chr5_1:52257824-52257843 Msa0736190:intron 15.0%
!!! TATTTTATTTTTTACCTTTG+GGG - chr5_1:52258351-52258370 None:intergenic 15.0%
!! AATAAACAAACTACTATATA+TGG + chr5_1:52258367-52258386 Msa0736190:intron 15.0%
!! TTGAATTATTTGATAGAAAA+TGG + chr5_1:52258768-52258787 Msa0736190:intron 15.0%
!!! ATAAGTTTTAAAAATACAGA+TGG + chr5_1:52259201-52259220 Msa0736190:intron 15.0%
!!! TAAGTTTTAAAAATACAGAT+GGG + chr5_1:52259202-52259221 Msa0736190:intron 15.0%
!! AAACAAATAAAAGAAAAACG+AGG - chr5_1:52255973-52255992 None:intergenic 20.0%
!! GTAAATATTGAAACAAAAGA+AGG - chr5_1:52256000-52256019 None:intergenic 20.0%
!!! TCGAAAATTATTTTTCGTTT+TGG + chr5_1:52256056-52256075 Msa0736190:intron 20.0%
!! AATTGGATAACAAAGTTAAA+TGG + chr5_1:52256237-52256256 Msa0736190:CDS 20.0%
!! TATGAGAAATTTGTGTATTT+AGG + chr5_1:52256350-52256369 Msa0736190:CDS 20.0%
!!! TAATATCGATTCAAAATTTG+AGG - chr5_1:52256555-52256574 None:intergenic 20.0%
!! TATAAAAAAGAACATAGCTT+GGG + chr5_1:52256745-52256764 Msa0736190:intron 20.0%
!!! AAATGTGTTTAAGATTGAAA+AGG - chr5_1:52256908-52256927 None:intergenic 20.0%
!!! ATTTTGTTATTGGAAAAGAT+TGG - chr5_1:52257124-52257143 None:intergenic 20.0%
!!! TGAACAAGTTATTTTGTTAT+TGG - chr5_1:52257134-52257153 None:intergenic 20.0%
!!! TATTTTCTTCTTCATAACAA+AGG - chr5_1:52257229-52257248 None:intergenic 20.0%
!!! TGGTTCTTAAATTTAGTTTA+TGG - chr5_1:52257313-52257332 None:intergenic 20.0%
!!! AGAAAAGTGTATAAATAAAG+GGG + chr5_1:52257760-52257779 Msa0736190:intron 20.0%
!! AAAGTGGAATATATTGTATT+GGG + chr5_1:52257783-52257802 Msa0736190:intron 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG + chr5_1:52257856-52257875 Msa0736190:intron 20.0%
!! GTTGTATTGAAAATTGAAAT+GGG + chr5_1:52257857-52257876 Msa0736190:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr5_1:52257893-52257912 Msa0736190:intron 20.0%
!!! AATAAAGCAAGTTAGTTTTT+TGG - chr5_1:52258002-52258021 None:intergenic 20.0%
!!! ACTAAAAAAAGATAATGGTT+GGG + chr5_1:52259118-52259137 Msa0736190:CDS 20.0%
!! CAAGAATTTAGAAAAATCAT+AGG - chr5_1:52259509-52259528 None:intergenic 20.0%
!!! CTATGATTTTTCTAAATTCT+TGG + chr5_1:52259507-52259526 Msa0736190:three_prime_UTR 20.0%
!!! TTTTTCTAAATTCTTGGTTT+AGG + chr5_1:52259513-52259532 Msa0736190:three_prime_UTR 20.0%
!!! TGTTCTTAATTTCTTGTTTT+AGG + chr5_1:52259597-52259616 Msa0736190:three_prime_UTR 20.0%
!!! TTTTAGTTGTTAGTAGTGAT+GGG - chr5_1:52255362-52255381 None:intergenic 25.0%
! TAACATATATAAGTTCCTCT+TGG + chr5_1:52255460-52255479 Msa0736190:five_prime_UTR 25.0%
!!! TTTCATCATTGATTCGAATT+AGG - chr5_1:52255843-52255862 None:intergenic 25.0%
!! GAGAATAATTTTACGAATCT+AGG + chr5_1:52255865-52255884 Msa0736190:intron 25.0%
!! TTTTGTGTCAATTAGGTTTT+CGG + chr5_1:52255894-52255913 Msa0736190:intron 25.0%
! CAAAACGAAAAATAATGCAT+CGG - chr5_1:52255948-52255967 None:intergenic 25.0%
! AAATTTGTGTATTTAGGCAT+TGG + chr5_1:52256356-52256375 Msa0736190:CDS 25.0%
! AATTTGTGTATTTAGGCATT+GGG + chr5_1:52256357-52256376 Msa0736190:CDS 25.0%
! ATTGTGTATAATAGCTAGAA+TGG - chr5_1:52256639-52256658 None:intergenic 25.0%
! GTATAAAAAAGAACATAGCT+TGG + chr5_1:52256744-52256763 Msa0736190:intron 25.0%
!! TAATCATTCATTTGTTGCAA+AGG + chr5_1:52256795-52256814 Msa0736190:intron 25.0%
!! TAAAGTACTATGTTAGACTT+TGG - chr5_1:52256998-52257017 None:intergenic 25.0%
!! ATATGACATTTTGTTGCAAT+TGG - chr5_1:52257195-52257214 None:intergenic 25.0%
!!! GTTTATGGTTTTTTTGTTGT+AGG - chr5_1:52257298-52257317 None:intergenic 25.0%
!! AAACTTTTTGAATTATGCCT+CGG + chr5_1:52257560-52257579 Msa0736190:intron 25.0%
! ATTGAATGAAAAATGAACAC+AGG + chr5_1:52257584-52257603 Msa0736190:intron 25.0%
! GAAAGTGGAATATATTGTAT+TGG + chr5_1:52257782-52257801 Msa0736190:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + chr5_1:52257892-52257911 Msa0736190:intron 25.0%
! TGTGATTGATTAGAATGTTA+AGG + chr5_1:52258071-52258090 Msa0736190:intron 25.0%
!! TTGTTCTAATTTTGTGTCTT+TGG + chr5_1:52258405-52258424 Msa0736190:intron 25.0%
!! TGTTCTAATTTTGTGTCTTT+GGG + chr5_1:52258406-52258425 Msa0736190:intron 25.0%
!!! TTATTTTTACCTTGCTCAAA+AGG - chr5_1:52258573-52258592 None:intergenic 25.0%
! ATATGAAAATTACATGAACC+TGG + chr5_1:52258628-52258647 Msa0736190:intron 25.0%
!! GTAGTTTACTATTAGTTTGA+TGG + chr5_1:52258036-52258055 Msa0736190:intron 25.0%
! GATTAAAATGCTATCTATGA+TGG + chr5_1:52258790-52258809 Msa0736190:intron 25.0%
! AAACAAACTATCTATCTATG+AGG + chr5_1:52258822-52258841 Msa0736190:intron 25.0%
! AACAAACTATCTATCTATGA+GGG + chr5_1:52258823-52258842 Msa0736190:intron 25.0%
!! TATCTGTTCTGATTTTTACA+TGG + chr5_1:52258863-52258882 Msa0736190:intron 25.0%
! AGAAAAACACTATAATGAGA+AGG - chr5_1:52259055-52259074 None:intergenic 25.0%
!! TCCTAATTTGCAGAATAAAT+GGG + chr5_1:52259091-52259110 Msa0736190:intron 25.0%
!! GACTAAAAAAAGATAATGGT+TGG + chr5_1:52259117-52259136 Msa0736190:CDS 25.0%
! ATGGTGTATATACTATATAG+TGG + chr5_1:52259570-52259589 Msa0736190:three_prime_UTR 25.0%
! TGGTGTATATACTATATAGT+GGG + chr5_1:52259571-52259590 Msa0736190:three_prime_UTR 25.0%
!! GTTTAAATGGGAAAAGTTTT+TGG - chr5_1:52259819-52259838 None:intergenic 25.0%
!!! GTTTTAGTTGTTAGTAGTGA+TGG - chr5_1:52255363-52255382 None:intergenic 30.0%
! GTTTCTGTTTTGTGTCAATT+AGG + chr5_1:52255887-52255906 Msa0736190:intron 30.0%
!! CGATGCATTATTTTTCGTTT+TGG + chr5_1:52255946-52255965 Msa0736190:intron 30.0%
! ATTACAGTGTATGCTTTGTT+AGG + chr5_1:52256180-52256199 Msa0736190:intron 30.0%
! ATTTGTGTATTTAGGCATTG+GGG + chr5_1:52256358-52256377 Msa0736190:CDS 30.0%
! ATAGCTAGAATGGATTCATT+TGG - chr5_1:52256629-52256648 None:intergenic 30.0%
!!! TCGCAGCAACATTTTTTTAT+AGG - chr5_1:52256715-52256734 None:intergenic 30.0%
GCAACAAATGAATGATTAAC+AGG - chr5_1:52256793-52256812 None:intergenic 30.0%
CTACAGTGAAGAAATGAAAT+CGG - chr5_1:52256863-52256882 None:intergenic 30.0%
CTAACATAGTACTTTAACAG+AGG + chr5_1:52257002-52257021 Msa0736190:intron 30.0%
TTCTTCTTCTTCTTCTTTGT+TGG - chr5_1:52257333-52257352 None:intergenic 30.0%
!! TCATTTTTCATTCAATACCG+AGG - chr5_1:52257580-52257599 None:intergenic 30.0%
CAAAGTAGGTCAATTAAACT+GGG - chr5_1:52257723-52257742 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG + chr5_1:52257767-52257786 Msa0736190:intron 30.0%
! TTGAAATGGGTCAGTTATTT+TGG + chr5_1:52257870-52257889 Msa0736190:intron 30.0%
!! TGAAATGGGTCAGTTATTTT+GGG + chr5_1:52257871-52257890 Msa0736190:intron 30.0%
!!! AAGTTAGTTTTTTGGCAAGT+TGG - chr5_1:52257994-52258013 None:intergenic 30.0%
TTGTGATGATTAGAAGGTTA+AGG + chr5_1:52258727-52258746 Msa0736190:intron 30.0%
! TTTTACATGGTATTCTACAG+TGG + chr5_1:52258876-52258895 Msa0736190:intron 30.0%
AAAGATGCAGTAGATATACA+TGG + chr5_1:52258923-52258942 Msa0736190:CDS 30.0%
AAGATGCAGTAGATATACAT+GGG + chr5_1:52258924-52258943 Msa0736190:CDS 30.0%
TAAATCAGAATCCATCCTTA+AGG + chr5_1:52258964-52258983 Msa0736190:CDS 30.0%
AAAATCAACATCCTTAAGGA+TGG - chr5_1:52258978-52258997 None:intergenic 30.0%
ACACTATAATGAGAAGGAAA+AGG - chr5_1:52259049-52259068 None:intergenic 30.0%
! GTCCTAATTTGCAGAATAAA+TGG + chr5_1:52259090-52259109 Msa0736190:intron 30.0%
! CCTAATTTGCAGAATAAATG+GGG + chr5_1:52259092-52259111 Msa0736190:intron 30.0%
! CTAATTTGCAGAATAAATGG+GGG + chr5_1:52259093-52259112 Msa0736190:intron 30.0%
GGGAGACTAAAAAAAGATAA+TGG + chr5_1:52259113-52259132 Msa0736190:CDS 30.0%
! AAAAAGATAATGGTTGGGTT+AGG + chr5_1:52259123-52259142 Msa0736190:CDS 30.0%
GGTTAATGAAAACATCAGAA+AGG + chr5_1:52259169-52259188 Msa0736190:CDS 30.0%
TTTAAAAATACAGATGGGAC+TGG + chr5_1:52259207-52259226 Msa0736190:intron 30.0%
!! TTTTGAAGGATAGAAAGTGA+AGG + chr5_1:52259429-52259448 Msa0736190:CDS 30.0%
AATTCTTGGTTTAGGTGTAT+TGG + chr5_1:52259521-52259540 Msa0736190:three_prime_UTR 30.0%
GGTGTATATACTATATAGTG+GGG + chr5_1:52259572-52259591 Msa0736190:three_prime_UTR 30.0%
!! AAGATGCTTGTTATCAGAGA+GGG + chr5_1:52255422-52255441 Msa0736190:five_prime_UTR 35.0%
ACAAACAAAGTTCCTCTACT+TGG - chr5_1:52255523-52255542 None:intergenic 35.0%
TCCAAGAATCTCACAATCAA+TGG + chr5_1:52255599-52255618 Msa0736190:intron 35.0%
! ATCGCGATTACGAATTGTTT+TGG + chr5_1:52255800-52255819 Msa0736190:intron 35.0%
CGGATATACACTAATTGCAT+CGG - chr5_1:52255928-52255947 None:intergenic 35.0%
! ATGGATTCATTTGGTGATAG+AGG - chr5_1:52256620-52256639 None:intergenic 35.0%
! TTTTCATTATCTCCCAAACC+TGG + chr5_1:52256661-52256680 Msa0736190:intron 35.0%
! TTTTCTCTATCTCCAGGTTT+GGG - chr5_1:52256676-52256695 None:intergenic 35.0%
! TTGGGTTGTTTGAGTAAGTT+AGG + chr5_1:52256763-52256782 Msa0736190:intron 35.0%
! TTGAAAAGGAAAAGCACACA+TGG - chr5_1:52256894-52256913 None:intergenic 35.0%
CAAAGCTGGATATTTGTTCT+AGG - chr5_1:52257042-52257061 None:intergenic 35.0%
AGGAGGATATTTGTTCTATG+TGG - chr5_1:52257070-52257089 None:intergenic 35.0%
AGATTGGAAGAGATAAGGAT+CGG - chr5_1:52257108-52257127 None:intergenic 35.0%
GGAAAAGATTGGAAGAGATA+AGG - chr5_1:52257113-52257132 None:intergenic 35.0%
! GACATTTTGTTGCAATTGGA+GGG - chr5_1:52257191-52257210 None:intergenic 35.0%
! TGACATTTTGTTGCAATTGG+AGG - chr5_1:52257192-52257211 None:intergenic 35.0%
CCAAAGTAGGTCAATTAAAC+TGG - chr5_1:52257724-52257743 None:intergenic 35.0%
CCAGTTTAATTGACCTACTT+TGG + chr5_1:52257721-52257740 Msa0736190:intron 35.0%
TCTGTGTGAAAAACCAAAGT+AGG - chr5_1:52257737-52257756 None:intergenic 35.0%
AGAAAATGGGTCAGTTATTC+CGG + chr5_1:52257906-52257925 Msa0736190:intron 35.0%
! AAAAAGCACAAAAGCTGAAG+AGG + chr5_1:52258267-52258286 Msa0736190:CDS 35.0%
! AAAAGCACAAAAGCTGAAGA+GGG + chr5_1:52258268-52258287 Msa0736190:CDS 35.0%
AATCATTGAAAAAGCCCCAA+AGG + chr5_1:52258334-52258353 Msa0736190:CDS 35.0%
AGAAGAGAGCAAATTACCTT+TGG - chr5_1:52258459-52258478 None:intergenic 35.0%
! TTATTCATGCCTTTTGAGCA+AGG + chr5_1:52258561-52258580 Msa0736190:intron 35.0%
! TGCTGTTTGTGATGATTAGA+AGG + chr5_1:52258721-52258740 Msa0736190:intron 35.0%
TTCAGCATCAATTCCAAATC+TGG - chr5_1:52258905-52258924 None:intergenic 35.0%
AGATGCAGTAGATATACATG+GGG + chr5_1:52258925-52258944 Msa0736190:CDS 35.0%
GAGCAAAATCAACATCCTTA+AGG - chr5_1:52258982-52259001 None:intergenic 35.0%
ATCATGAGTCCATTTGTGAA+GGG - chr5_1:52259005-52259024 None:intergenic 35.0%
CCCCATTTATTCTGCAAATT+AGG - chr5_1:52259095-52259114 None:intergenic 35.0%
ATCCTAAGAAGAAGACTGTA+CGG + chr5_1:52259147-52259166 Msa0736190:CDS 35.0%
! GTTCGAAAATTGCTGTTAAG+AGG - chr5_1:52259367-52259386 None:intergenic 35.0%
! AACAGCAATTTTCGAACAGA+TGG + chr5_1:52259370-52259389 Msa0736190:CDS 35.0%
! GGAAATTGTGACTCTTTTGA+AGG + chr5_1:52259415-52259434 Msa0736190:CDS 35.0%
TATAGTAGTGCATGTGGATT+AGG + chr5_1:52259666-52259685 Msa0736190:three_prime_UTR 35.0%
! GAATGAGTGTTATTGTTCTG+TGG + chr5_1:52259701-52259720 Msa0736190:three_prime_UTR 35.0%
TATAGTAGTGCATGTGGATT+AGG + chr5_1:52259666-52259685 Msa0736190:three_prime_UTR 35.0%
! AATTTTGTACATAGCAGCAG+TGG - chr5_1:52259782-52259801 None:intergenic 35.0%
! GATGGGAAAGAAGTATAGAG+AGG - chr5_1:52255345-52255364 None:intergenic 40.0%
!! CAAGATGCTTGTTATCAGAG+AGG + chr5_1:52255421-52255440 Msa0736190:five_prime_UTR 40.0%
GATTGTGAGATTCTTGGAGA+AGG - chr5_1:52255597-52255616 None:intergenic 40.0%
! GCCATTGATTGTGAGATTCT+TGG - chr5_1:52255603-52255622 None:intergenic 40.0%
! TGTGTCAATTAGGTTTTCGG+TGG + chr5_1:52255897-52255916 Msa0736190:intron 40.0%
! CTTTTCTCTATCTCCAGGTT+TGG - chr5_1:52256677-52256696 None:intergenic 40.0%
! TTGAGCTTTTCTCTATCTCC+AGG - chr5_1:52256682-52256701 None:intergenic 40.0%
GAAGAAGAAGAAGATGCCAA+AGG + chr5_1:52257341-52257360 Msa0736190:intron 40.0%
AAGATGCCAAAGGTAGAGAT+AGG + chr5_1:52257351-52257370 Msa0736190:intron 40.0%
AGAAGAAGAAGAAGCTACAC+TGG + chr5_1:52257402-52257421 Msa0736190:intron 40.0%
!!! CACTGGTTTTCAACGTTGTA+TGG + chr5_1:52257419-52257438 Msa0736190:intron 40.0%
TCAACGTTGTATGGTGTGAA+TGG + chr5_1:52257428-52257447 Msa0736190:intron 40.0%
GTAGGTCAATTAAACTGGGA+CGG - chr5_1:52257719-52257738 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG + chr5_1:52257907-52257926 Msa0736190:intron 40.0%
! CTAATTTTGTGTCTTTGGGC+AGG + chr5_1:52258410-52258429 Msa0736190:intron 40.0%
! ATTTTGCTCCCCTTCACAAA+TGG + chr5_1:52258993-52259012 Msa0736190:CDS 40.0%
TCATGAGTCCATTTGTGAAG+GGG - chr5_1:52259004-52259023 None:intergenic 40.0%
CATCATGAGTCCATTTGTGA+AGG - chr5_1:52259006-52259025 None:intergenic 40.0%
TCCTAAGAAGAAGACTGTAC+GGG + chr5_1:52259148-52259167 Msa0736190:CDS 40.0%
TTTCCATCGACAGACTTCTT+TGG + chr5_1:52259268-52259287 Msa0736190:intron 40.0%
TGAAGGATAGAAAGTGAAGG+TGG + chr5_1:52259432-52259451 Msa0736190:exon 40.0%
TTGCTCGTGAATGTGATTCA+TGG + chr5_1:52259551-52259570 Msa0736190:three_prime_UTR 40.0%
CACAGAACAATAACACTCAC+TGG - chr5_1:52259641-52259660 None:intergenic 40.0%
! CAGTGAGTGTTATTGTTCTG+TGG + chr5_1:52259639-52259658 Msa0736190:three_prime_UTR 40.0%
! ACTCTAGAAAGTTCGCCAAG+AGG - chr5_1:52255478-52255497 None:intergenic 45.0%
GTTTGTCGAGAGATCAGAGA+CGG + chr5_1:52255537-52255556 Msa0736190:five_prime_UTR 45.0%
AGAGGCAGCAACAAATTCTC+AGG + chr5_1:52255562-52255581 Msa0736190:five_prime_UTR 45.0%
AGATCGCAAGATGAAGTCTG+TGG + chr5_1:52256208-52256227 Msa0736190:CDS 45.0%
CAAGATGAAGTCTGTGGCAT+TGG + chr5_1:52256214-52256233 Msa0736190:CDS 45.0%
! GAAGTCTGTGGCATTGGAAT+TGG + chr5_1:52256220-52256239 Msa0736190:CDS 45.0%
CATTGGGGAACGTGAACTAA+AGG + chr5_1:52256373-52256392 Msa0736190:CDS 45.0%
TCTATGTGGTGACACAAAGC+TGG - chr5_1:52257056-52257075 None:intergenic 45.0%
ATGCCAAAGGTAGAGATAGG+TGG + chr5_1:52257354-52257373 Msa0736190:intron 45.0%
ACGTTGTATGGTGTGAATGG+AGG + chr5_1:52257431-52257450 Msa0736190:intron 45.0%
CGTTGTATGGTGTGAATGGA+GGG + chr5_1:52257432-52257451 Msa0736190:intron 45.0%
ATGGTGTGAATGGAGGGAAA+TGG + chr5_1:52257438-52257457 Msa0736190:intron 45.0%
GTCAATTAAACTGGGACGGA+GGG - chr5_1:52257715-52257734 None:intergenic 45.0%
TATTAATACTCCCTCCGTCC+CGG - chr5_1:52257928-52257947 None:intergenic 45.0%
TTGTCTGCTGCAGCTTAAGT+GGG + chr5_1:52258181-52258200 Msa0736190:intron 45.0%
TAAGTGGGAAGCTTGTAAGC+TGG + chr5_1:52258196-52258215 Msa0736190:CDS 45.0%
TGTAAGCTGGCTAGGTCTAA+TGG + chr5_1:52258209-52258228 Msa0736190:CDS 45.0%
GTAAGCTGGCTAGGTCTAAT+GGG + chr5_1:52258210-52258229 Msa0736190:CDS 45.0%
ATTAGACCTAGCTAGCTGAC+GGG - chr5_1:52258297-52258316 None:intergenic 45.0%
CATTAGACCTAGCTAGCTGA+CGG - chr5_1:52258298-52258317 None:intergenic 45.0%
AGCTAGCTAGGTCTAATGCA+GGG + chr5_1:52258300-52258319 Msa0736190:CDS 45.0%
CATTGGGAAACGTGAACCAA+AGG + chr5_1:52258440-52258459 Msa0736190:CDS 45.0%
ACCTGGCTACATGAACTTGA+AGG + chr5_1:52258645-52258664 Msa0736190:intron 45.0%
CCTGGCTACATGAACTTGAA+GGG + chr5_1:52258646-52258665 Msa0736190:intron 45.0%
! TCTACAGTGGACTCCAGATT+TGG + chr5_1:52258889-52258908 Msa0736190:intron 45.0%
TGCAGTAGATATACATGGGG+AGG + chr5_1:52258928-52258947 Msa0736190:CDS 45.0%
! AATGGACTCATGATGCACTG+AGG + chr5_1:52259011-52259030 Msa0736190:CDS 45.0%
ATGGACTCATGATGCACTGA+GGG + chr5_1:52259012-52259031 Msa0736190:CDS 45.0%
ACCCGTACAGTCTTCTTCTT+AGG - chr5_1:52259152-52259171 None:intergenic 45.0%
! AATACAGATGGGACTGGTAC+TGG + chr5_1:52259213-52259232 Msa0736190:intron 45.0%
CATCCAAAGAAGTCTGTCGA+TGG - chr5_1:52259274-52259293 None:intergenic 45.0%
GTTAAGAGGTTCACAACAGC+AGG - chr5_1:52259353-52259372 None:intergenic 45.0%
TACTGCGAATGCAGGTATCA+AGG + chr5_1:52259394-52259413 Msa0736190:CDS 45.0%
CAAAACTCAACCACTCCTTC+CGG - chr5_1:52259471-52259490 None:intergenic 45.0%
GGTGTCTATAGTAGTGCATG+TGG + chr5_1:52259660-52259679 Msa0736190:three_prime_UTR 45.0%
GGTGTCTATAGTAGTGCATG+TGG + chr5_1:52259660-52259679 Msa0736190:three_prime_UTR 45.0%
TTTGTACATAGCAGCAGTGG+TGG - chr5_1:52259779-52259798 None:intergenic 45.0%
TGCTCTGCACTTCCAAGTAG+AGG + chr5_1:52255508-52255527 Msa0736190:five_prime_UTR 50.0%
GAGAGATCAGAGACGGTTAG+AGG + chr5_1:52255544-52255563 Msa0736190:five_prime_UTR 50.0%
ATGGATCTGATGACTCCAGC+TGG + chr5_1:52256303-52256322 Msa0736190:CDS 50.0%
! CTCCAGCTGGAGTCAGATTT+TGG + chr5_1:52256316-52256335 Msa0736190:CDS 50.0%
AGCCAAAATCTGACTCCAGC+TGG - chr5_1:52256321-52256340 None:intergenic 50.0%
! ATCGGCTGGGTTGACAAAGA+AGG - chr5_1:52257090-52257109 None:intergenic 50.0%
GGAAGAGATAAGGATCGGCT+GGG - chr5_1:52257103-52257122 None:intergenic 50.0%
TGGAAGAGATAAGGATCGGC+TGG - chr5_1:52257104-52257123 None:intergenic 50.0%
CCACCACCTATCTCTACCTT+TGG - chr5_1:52257360-52257379 None:intergenic 50.0%
CCAAAGGTAGAGATAGGTGG+TGG + chr5_1:52257357-52257376 Msa0736190:intron 50.0%
! AAGGTAGAGATAGGTGGTGG+AGG + chr5_1:52257360-52257379 Msa0736190:intron 50.0%
GGTCAATTAAACTGGGACGG+AGG - chr5_1:52257716-52257735 None:intergenic 50.0%
ATGGGTCAGTTATTCCGGGA+CGG + chr5_1:52257911-52257930 Msa0736190:intron 50.0%
CACTTAAGCTGCAGCAGACA+AGG - chr5_1:52258182-52258201 None:intergenic 50.0%
CTTGTCTGCTGCAGCTTAAG+TGG + chr5_1:52258180-52258199 Msa0736190:intron 50.0%
!! GGCTAGGTCTAATGGGTTTG+AGG + chr5_1:52258217-52258236 Msa0736190:CDS 50.0%
CAGCTAGCTAGGTCTAATGC+AGG + chr5_1:52258299-52258318 Msa0736190:CDS 50.0%
CTAGGTCTAATGCAGGGACT+CGG + chr5_1:52258306-52258325 Msa0736190:CDS 50.0%
TAGGTCTAATGCAGGGACTC+GGG + chr5_1:52258307-52258326 Msa0736190:CDS 50.0%
TTGTGTCTTTGGGCAGGCAT+TGG + chr5_1:52258416-52258435 Msa0736190:intron 50.0%
TGTGTCTTTGGGCAGGCATT+GGG + chr5_1:52258417-52258436 Msa0736190:intron 50.0%
! CCCTTCAAGTTCATGTAGCC+AGG - chr5_1:52258649-52258668 None:intergenic 50.0%
CCAGGCTCAGCAGAAGAATT+AGG - chr5_1:52259299-52259318 None:intergenic 50.0%
CCTAATTCTTCTGCTGAGCC+TGG + chr5_1:52259296-52259315 Msa0736190:CDS 50.0%
CAGATGGTTACTGCGAATGC+AGG + chr5_1:52259386-52259405 Msa0736190:CDS 50.0%
AGGTGGAAAGAGTAGCTAGC+CGG + chr5_1:52259449-52259468 Msa0736190:three_prime_UTR 50.0%
GTCTCTGCTTGAAGACGCTG+TGG + chr5_1:52256274-52256293 Msa0736190:CDS 55.0%
GAAGACGCTGTGGCAGACTA+TGG + chr5_1:52256284-52256303 Msa0736190:CDS 55.0%
! GGCTGGGTTGACAAAGAAGG+AGG - chr5_1:52257087-52257106 None:intergenic 55.0%
!! GTAGAGATAGGTGGTGGAGG+AGG + chr5_1:52257363-52257382 Msa0736190:intron 55.0%
GTCAGTTATTCCGGGACGGA+GGG + chr5_1:52257915-52257934 Msa0736190:intron 55.0%
GGGAAGCTTGTAAGCTGGCT+AGG + chr5_1:52258201-52258220 Msa0736190:CDS 55.0%
AGGTCTAATGCAGGGACTCG+GGG + chr5_1:52258308-52258327 Msa0736190:CDS 55.0%
!! TTTGGGCAGGCATTGGGCAT+TGG + chr5_1:52258423-52258442 Msa0736190:intron 55.0%
!! TTGGGCAGGCATTGGGCATT+GGG + chr5_1:52258424-52258443 Msa0736190:intron 55.0%
TTCTGCTGTGCCTGCTGTGA+TGG + chr5_1:52259322-52259341 Msa0736190:CDS 55.0%
GGAAAGAGTAGCTAGCCGGA+AGG + chr5_1:52259453-52259472 Msa0736190:three_prime_UTR 55.0%
!! GAGATAGGTGGTGGAGGAGG+AGG + chr5_1:52257366-52257385 Msa0736190:intron 60.0%
GGTCAGTTATTCCGGGACGG+AGG + chr5_1:52257914-52257933 Msa0736190:intron 60.0%
!! GCAGGCACAGCAGAAGTACC+AGG - chr5_1:52259317-52259336 None:intergenic 60.0%
GCAGGCACATCCATCACAGC+AGG - chr5_1:52259335-52259354 None:intergenic 60.0%
GAGTAGCTAGCCGGAAGGAG+TGG + chr5_1:52259458-52259477 Msa0736190:three_prime_UTR 60.0%
GGGAAGCCCGTCAGCTAGCT+AGG + chr5_1:52258288-52258307 Msa0736190:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 52255287 52259849 52255287 ID=Msa0736190;Name=Msa0736190
chr5_1 mRNA 52255287 52259849 52255287 ID=Msa0736190-mRNA-1;Parent=Msa0736190;Name=Msa0736190-mRNA-1;_AED=0.29;_eAED=0.29;_QI=298|1|1|1|0.83|0.85|7|401|258
chr5_1 exon 52255287 52255583 52255287 ID=Msa0736190-mRNA-1:exon:14736;Parent=Msa0736190-mRNA-1
chr5_1 exon 52256202 52256394 52256202 ID=Msa0736190-mRNA-1:exon:14737;Parent=Msa0736190-mRNA-1
chr5_1 exon 52258194 52258355 52258194 ID=Msa0736190-mRNA-1:exon:14738;Parent=Msa0736190-mRNA-1
chr5_1 exon 52258432 52258461 52258432 ID=Msa0736190-mRNA-1:exon:14739;Parent=Msa0736190-mRNA-1
chr5_1 exon 52258896 52259033 52258896 ID=Msa0736190-mRNA-1:exon:14740;Parent=Msa0736190-mRNA-1
chr5_1 exon 52259104 52259190 52259104 ID=Msa0736190-mRNA-1:exon:14741;Parent=Msa0736190-mRNA-1
chr5_1 exon 52259281 52259849 52259281 ID=Msa0736190-mRNA-1:exon:14742;Parent=Msa0736190-mRNA-1
chr5_1 five_prime_UTR 52255287 52255583 52255287 ID=Msa0736190-mRNA-1:five_prime_utr;Parent=Msa0736190-mRNA-1
chr5_1 five_prime_UTR 52256202 52256202 52256202 ID=Msa0736190-mRNA-1:five_prime_utr;Parent=Msa0736190-mRNA-1
chr5_1 CDS 52256203 52256394 52256203 ID=Msa0736190-mRNA-1:cds;Parent=Msa0736190-mRNA-1
chr5_1 CDS 52258194 52258355 52258194 ID=Msa0736190-mRNA-1:cds;Parent=Msa0736190-mRNA-1
chr5_1 CDS 52258432 52258461 52258432 ID=Msa0736190-mRNA-1:cds;Parent=Msa0736190-mRNA-1
chr5_1 CDS 52258896 52259033 52258896 ID=Msa0736190-mRNA-1:cds;Parent=Msa0736190-mRNA-1
chr5_1 CDS 52259104 52259190 52259104 ID=Msa0736190-mRNA-1:cds;Parent=Msa0736190-mRNA-1
chr5_1 CDS 52259281 52259448 52259281 ID=Msa0736190-mRNA-1:cds;Parent=Msa0736190-mRNA-1
chr5_1 three_prime_UTR 52259449 52259849 52259449 ID=Msa0736190-mRNA-1:three_prime_utr;Parent=Msa0736190-mRNA-1
Gene Sequence

>Msa0736190

ATGAAAGATCGCAAGATGAAGTCTGTGGCATTGGAATTGGATAACAAAGTTAAATGGAGCACGCTTAGAGAGTCTCTGCTTGAAGACGCTGTGGCAGACTATGGATCTGATGACTCCAGCTGGAGTCAGATTTTGGCTGATGAGAAGTATGAGAAATTTGTGTATTTAGGCATTGGGGAACGTGAACTAAAGCTTAAGTGGGAAGCTTGTAAGCTGGCTAGGTCTAATGGGTTTGAGGATGAAAGTAGCGAAGAAGCTGAGAAGAAAAAAGCACAAAAGCTGAAGAGGGAAGCCCGTCAGCTAGCTAGGTCTAATGCAGGGACTCGGGGTGTAATCATTGAAAAAGCCCCAAAGGCATTGGGCATTGGGAAACGTGAACCAAAGTGGACTCCAGATTTGGAATTGATGCTGAAAGATGCAGTAGATATACATGGGGAGGATCATGCTGATTTTAAATCAGAATCCATCCTTAAGGATGTTGATTTTGCTCCCCTTCACAAATGGACTCATGATGCACTGAGGAATAAATGGGGGAGACTAAAAAAAGATAATGGTTGGGTTAGGAATCCTAAGAAGAAGACTGTACGGGTTAATGAAAACATCAGAAAGACTTCTTTGGATGTGCCTAATTCTTCTGCTGAGCCTGGTACTTCTGCTGTGCCTGCTGTGATGGATGTGCCTGCTGTTGTGAACCTCTTAACAGCAATTTTCGAACAGATGGTTACTGCGAATGCAGGTATCAAGGAAATTGTGACTCTTTTGAAGGATAGAAAGTGA
Protein sequence

>Msa0736190

MKDRKMKSVALELDNKVKWSTLRESLLEDAVADYGSDDSSWSQILADEKYEKFVYLGIGERELKLKWEACKLARSNGFEDESSEEAEKKKAQKLKREARQLARSNAGTRGVIIEKAPKALGIGKREPKWTPDLELMLKDAVDIHGEDHADFKSESILKDVDFAPLHKWTHDALRNKWGRLKKDNGWVRNPKKKTVRVNENIRKTSLDVPNSSAEPGTSAVPAVMDVPAVVNLLTAIFEQMVTANAGIKEIVTLLKDRK*